Use of juglone in preparation of medicines treating brain tumor
A technology of jugone and brain tumor, which is applied in the field of jugone in the preparation of drugs for the treatment of brain tumors, can solve the problems of unclear specific mechanism of anti-brain tumor effect and the like
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Embodiment 1
[0022] Example 1 In vitro killing test
[0023] The experiment used U87 glioblastoma cell line (obtainable through commercial channels). When it grew to 80% confluence, the culture medium in the bottle was aspirated, washed gently with PBS solution twice, and fully digested with 0.25% trypsin to obtain Place the cell suspension in a 15ml centrifuge tube and centrifuge at 800 rpm for 5 minutes, discard the supernatant, add 5ml of complete medium to resuspend the cells, draw a drop of the cell suspension, place it on a cell counting plate, and add trypan blue staining solution for counting under a microscope , the number of unstained cells should not be less than 95%. According to the counting results, the cell suspension was diluted to 2×10 with complete medium in proportion. 4 Inoculate into a 96-well plate at a concentration of 1 / ml, add 150ul of cell suspension to each well, add complete medium to the zero well without cells, and add 250ul of PBS to the remaining wells to ...
Embodiment 2
[0024] Embodiment 2 Animal experiment
[0025] After weighing 20g female nude mice at the age of 6 weeks, intraperitoneally inject diluted chloral hydrate solution at a rate of 100 mg / kg. After the mice are anesthetized successfully, they are fixed in a stereotaxic injection apparatus for the brain. After the scalp is disinfected, a median incision of about 5mm long, expose the sagittal suture, coronal suture and bregma, take 2.3mm lateral to the midline, 0.1mm in front of the coronal suture as the puncture point, drill a micro drill hole, extract 5ul cell suspension with a 10ul micro sampler, vertically Insert the needle at a depth of 3.3mm and retreat 0.5mm. Under the control of the multi-channel micro-injection pump, inject the cell suspension at a speed of 1ul / min. After the injection is completed, keep the needle for 5 minutes, slowly pull out the micro-injector, and seal it with bone wax After the puncture point and the operation field were disinfected again, the opera...
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