16 results about "Forsythoside A" patented technology

The invention relates to the technical field of synthetic biology and microbial engineering, in particular to recombinant escherichia coli for de novo synthesis of forsythiaside A. The invention provides recombinant escherichia coli for de novo synthesis of forsythiaside A. A preparation method of the recombinant escherichia coli comprises a step of improving an escherichia coli starting strain, and the escherichia coli starting strain is escherichia coli MG1655. In the fermentation process of the recombinant escherichia coli, effective accumulation of forsythiaside A in fermentation liquor can be achieved, and a foundation can be laid for subsequent construction of high-yield forsythiaside A engineering bacteria.

The invention relates to the technical field of traditional Chinese medicine compositions, and provides a traditional Chinese medicine composition for removing acnes, which is prepared from the following components in parts by weight: 1-2 parts of honeysuckle stem extract, 0.3-0.8 part of fructus forsythiae extract, 2-4 parts of radix scutellariae extract, 0.5-3.5 parts of cortex magnoliae officinalis extract and 1-5 parts of astragalus complanatus extract. The preparation method of the fructus forsythiae extract comprises the following steps: crushing fructus forsythiae, performing steam explosion treatment to obtain pretreated fructus forsythiae, adding water, adding biological compound enzyme, performing enzymolysis, filtering and performing enzyme deactivation to obtain filtrate; enabling the filtrate to pass through macroporous resin, washing with water, carrying out first elution to obtain a solution I, and carrying out second elution to obtain a solution II; performing post-treatment on the solution I to obtain forsythiaside; performing post-treatment on the solution II to obtain forsythin; the biological compound enzyme comprises alpha-galactosidase, lipase and xylanase. Through the technical scheme, the problem of low extraction rate of forsythin and forsythiaside in fructus forsythiae in the prior art is solved.

The invention discloses an establishment method and an application method of a fingerprint spectrum of a honeysuckle and fructus forsythiae mixture, and belongs to the technical field of detection of effective components of traditional Chinese medicine, and the establishment method comprises the following steps: S1, determining chromatographic conditions; s2, preparing a test solution and a mixed reference solution; s3, collecting chromatograms of the test sample and the mixed reference substance; and S4, matching common peaks, calculating the relative retention time and the relative peak area of each common peak, comparing with the chromatogram of the reference substance, identifying the common peaks, and establishing the fingerprint spectrum of the honeysuckle and fructus forsythiae mixture. According to the establishment method of the fingerprint spectrum of the honeysuckle and fructus forsythiae mixture, reference and basis are provided for quality control of the honeysuckle and fructus forsythiae mixture, and the establishment method has the advantages of being simple, convenient, stable, high in precision, good in reproducibility and the like. The fingerprint spectrum has 11 effectively separated common peaks, identifies the characteristic peaks of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, liquiritin and forsythiaside A, can reflect the condition of the effective components of the honeysuckle and fructus forsythiae mixture, and effectively characterizes the quality of the honeysuckle and fructus forsythiae mixture.

The invention discloses an application of forsythiaside A in preparation of an inhibitor for treating methicillin-resistant staphylococcus aureus Stp1. Firstly, an Stp1 protein is purified for an Stp1 phosphatase activity experiment, and it is found that the protein activity is reduced along with increase of the drug concentration, and the IC50 value is 2.673 micrograms / mL. Then, the forsythiaside A with different concentrations and the USA300 strain are co-cultured, and it is found that the forsythiaside A cannot inhibit growth of the USA300 strain. In addition, in-vitro experiments show that forsythiaside A shows a good cell protection effect, and staphylococcus aureus mediated cell damage is effectively relieved; in-vivo experiments show that the forsythiaside A relieves infection caused by methicillin-resistant staphylococcus aureus, and lesions of main organs (liver, kidney and lung) of mice are improved. Therefore, the forsythiaside A does not cause the generation of bacterial drug resistance by targeting the staphylococcus aureus Stp1, and has important significance in the aspect of treating infection caused by methicillin-resistant staphylococcus aureus.

The invention belongs to the technical field of medicines, and particularly relates to forsythiaside glucosamine, a preparation method thereof and application of the forsythiaside glucosamine in treatment of osteoarthritis. The invention relates to design and synthesis of forsythiaside glucosamine, and researches on the treatment effect of forsythiaside glucosamine on an osteoarthritis mouse model. In the structural general formula of the compound, R1 is an amino group, a phthaloyl amino group or an allyloxy amido group, R2, R3 and R4 groups represent a hydroxyl group or an alkanoyloxy group, and the configurations of different head positions are alpha and beta. Wherein the alkanoyloxy group is a C1-C6 alkanoyloxy group or a deuterated alkanoyloxy group. The synthesized forsythiaside glucosamine is used as a twin drug, and shows better cartilage generation promoting activity and anti-inflammatory activity compared with combined use of forsythiaside and glucosamine on an osteoarthritis mouse model. In addition, under the same administration dosage, the activity of promoting cartilage generation and the like is obviously superior to that of forsythiaside acetylglucosamine in the prior art.

The invention relates to application of forsythiaside in treating cerebral arterial thrombosis, and belongs to the technical field of biological medicine. The phillygenin is a small molecule compound extracted from traditional Chinese medicine fructus forsythiae, can be transported into the brain through LILRA5 protein on the surface of peripheral blood mononuclear cells, acts on astrocytes, inhibits combination of PTP1B and FGFR1, enhances phosphorylation of FGFR1, and activates a downstream protective signal channel, so that the neuroprotective effect is achieved. Experiments show that forsythiaside can significantly reduce the volume of cerebral infarction and improve neurological impairment. The invention provides new candidate molecules and mechanism targets for the drug treatment of cerebral arterial thrombosis.

This invention belongs to the pharmaceutical field, specifically relating to a composition for enhancing innate immunity and exerting anti-infective effects, and its uses. Specifically, this invention selects ALOX15 enzyme activity inhibitors, including baicalin; compounds that promote ALOX15 transcriptional activity, including aconitine; and compounds that inhibit ALOX15 protein degradation, including forsythoside A / E / I, and combines them in a multi-strategy scientific formulation. Experimental studies have shown that the composition containing ALOX15 enzyme activity inhibitors, ALOX15 transcriptional activity promoters, and ALOX15 protein degradation inhibitors can effectively enhance the body's innate immunity, increase the synthesis and secretion of IFN-β, and exert anti-pathogen infection effects. The composition of this invention can also inhibit the production of pathogen-induced inflammatory factors and has a therapeutic effect on pathogen-induced secondary inflammatory symptoms, showing great promise for clinical application.

An injectable hydrogel for pet endometritis is provided, belonging to the technical field of veterinary drug preparation. The injectable hydrogel includes the following raw materials: forsythiaside A, chitosan, sodium β-glycerophosphate, sodium hyaluronate, acetic acid, and water. By combining the technical advantages of thermosensitive gel with injectable gel, forsythiaside A is used in combination with the chitosan to obtain an injectable hydrogel with excellent antibacterial properties. Encapsulating forsythiaside A with the chitosan not only enhances an antibacterial effect of forsythiaside A, but also prolongs a release time of the drug and can effectively shorten a duration required for repairing inflammatory damages, thereby reducing the probability of secondary infection in the uterine cavity.

The invention belongs to the technical field of medicine. The invention discloses an application of forsythiaside A in preparation of a medicine for treating bacterial infectious pneumonia. The method comprises the following steps: firstly, establishing a model of mice bacterial infectious pneumonia caused by tracheal intubation instillation of Pseudomonas aeruginosa in vivo, and applying forsythiaside A to treat, so that the infection symptom of the model mice can be obviously relieved, and the lung inflammatory response can be effectively relieved. The method comprises the following steps: firstly, establishing a model of pulmonary alveolar macrophages infected with Pseudomonas aeruginosa, Klebsiella pneumoniae or Acinetobacter baumannii in vitro, and then, establishing a model of pulmonary alveolar macrophages infected with Pseudomonas aeruginosa, Klebsiella pneumoniae or Acinetobacter baumannii in vitro, so that after forsythiaside A is added, the damage of the macrophages can be effectively alleviated, and the number of infectious bacteria in cytoplasm can be reduced. Experimental results show that forsythiaside A can be effectively used for treating bacterial infectious pneumonia.

The invention discloses a method for synchronously and quantitatively detecting 14 components in fructus forsythiae based on ultra-high performance liquid chromatography, and belongs to the technical field of quality detection of traditional Chinese medicinal materials. Dissolving the fructus forsythiae powder in a methanol aqueous solution, and extracting to obtain a to-be-detected sample; the method comprises the following steps: respectively preparing a standard solution and a mixed standard solution by taking chlorogenic acid, forsythiaside I, forsythiaside B, rutin, forsythiaside A, forsythiaside H, tectoridin, pinoresinol-beta-D-glucoside, naringin, forsythin, arctiin, quercetin, kaempferol and forsythiaside as standard substances; the method comprises the following steps: acquiring a standard curve corresponding to each standard substance, a peak position and a chromatographic result of a to-be-detected sample solution through ultra-high performance liquid chromatography, and substituting the chromatographic result into a standard curve equation of the standard substance; and calculating the contents of chlorogenic acid, forsythiaside I, forsythiaside B, rutin, forsythiaside A, forsythiaside H, tectoridin, pinoresinol-beta-D-glucoside, naringin, forsythin, arctiin, quercetin, kaempferol and forsythiaside in the sample to be detected.

The invention discloses a method for treating fructus forsythiae leaves and extracting forsythiaside A and xylooligosaccharide at the same time by adopting a steam explosion technology and application of the method. The method comprises the following steps: pre-soaking fragments of the fructus forsythiae leaves, and then putting the fragments into a steam explosion storage tank for explosion, after the treatment, extracting to obtain crude extracts of forsythiaside A and xylooligosaccharide by using an alcohol extraction method and an acidolysis method respectively; according to the method, the fructus forsythiae leaves are treated by utilizing a steam explosion technology, and a strong shearing force is generated through instant pressure relief of high-temperature and high-pressure steam, so that crystal structures of lignocellulose are effectively damaged, glucosidic bond breakage of a hemicellulose main chain is promoted, the polymerization degree of xylan is reduced, and the yield of the xylan is increased. And when the xylooligosaccharide is generated, the loose porous structure formed by explosion is formed, and the surface area is increased, so that the dissolution of forsythiaside A is promoted. By means of the technology, 33.42 mg / g of forsythiaside A and about 33.3 g / kg of xylooligosaccharide can be obtained from fructus forsythiae leaves picked in August after blasting, and compared with unblasted raw materials, the content of forsythiaside A is increased by about 14.61%.

The invention discloses a fingerprint spectrum detection method for yin-nourishing and stomach-clearing granules based on multi-component substances, and belongs to the technical field of quality control of traditional Chinese medicine preparations. According to the method, a high performance liquid chromatography-mass spectrometry technology is adopted, 5-hydroxymethylfurfural, oxymatrine, neomangiferin, mangiferin, forsythiaside A, rutin, tectoridin, naringin, iridoside, naringenin and irisflorentin are taken as reference substances, different batches of test products are detected by optimizing extraction conditions and chromatographic parameters, and the fingerprint spectrum is successfully constructed. The method is simple and easy to implement, high in precision and good in reproducibility, multi-component synchronous identification can be achieved at a time, the detection efficiency is greatly improved, and time and cost are saved; quality monitoring can be accurately, rapidly and simultaneously carried out on a plurality of characteristic components of the Yin-nourishing and stomach-clearing granules, and a powerful guarantee is provided for controlling the quality and ensuring the clinical curative effect of the Yin-nourishing and stomach-clearing granules.

The invention belongs to the field of biological medicines, and particularly relates to a novel application of forsythin in preparation of a medicine for preventing and treating amyotrophy. In-vivo and in-vitro experiments prove that the phillyrin can remarkably improve the level of endogenous prostaglandin E2 by inhibiting the activity or expression of 15-hydroxyprostaglandin dehydrogenase in muscular tissues, so that the expression of muscular atrophy key factors FOXO3a and Atrogin-1 is inhibited, and finally the effects of protecting muscle quality and improving muscle functions are achieved. The invention reveals that forsythin has the functions of inhibiting 15-PGDH and relieving muscle atrophy for the first time, and brand new medical application is provided for forsythin. The invention provides a brand-new therapeutic scheme and pharmaceutical composition for treating sarcopenia by using forsythin as an active component and regulating the 15-PGDH / PGE2 pathway. The invention provides an important candidate compound for developing efficient and safe sarcopenia resisting medicines, and has important practical significance.

The invention relates to the technical field of fructus forsythiae extracts, in particular to a preparation method of a fructus forsythiae extract and application of the fructus forsythiae extract in shampoo, and the preparation method of the fructus forsythiae extract comprises the following steps: S1, pretreatment; s2, performing enzymolysis treatment; s3, microwave extraction; and S4, centrifugal purification. According to the method, the improved emulsion with the vitamin C-citric acid buffer solution as a water phase and the sunflower lecithin and the nonionic emulsifier as the compound emulsifier is used for low-temperature soaking treatment of the fructus forsythiae fruits, a brand-new extraction system is formed, the forsythin and the forsythiaside A in the fructus forsythiae can be effectively extracted, and the loss of the forsythin and the forsythiaside A can be reduced.

The invention belongs to the technical field of medicines, and particularly relates to application of forsythiaside A in preparation of a medicine for treating or preventing heart injury caused by adriamycin. The invention discloses the application of forsythiaside A in preparing the medicine for treating or preventing heart injury caused by adriamycin for the first time, and in-vivo and in-vitro experiments prove that forsythiaside A can perform reverse inhibition on an adriamycin cardiotoxicity mechanism and does not interfere with the killing effect of adriamycin on tumor cells under an effective dose; the problem of lack of adriamycin cardiotoxicity prevention and treatment means at present is solved, and a brand new solution and a new, safe and effective candidate pharmaceutical composition are provided for clinical prevention and treatment of adriamycin cardiotoxicity.

This invention relates to the field of biomedical technology, specifically to the application of forsythoside in the preparation of drugs for the prevention and treatment of infectious bronchitis in chickens. Immunofluorescence and Western blotting results show that forsythoside can significantly inhibit the expression of viral nucleocapsid proteins in CEK cells and reduce the viral load in CEK cells. Furthermore, forsythoside can protect against IBV infection in broiler chickens. Quantitative fluorescence results indicate a significant reduction in viral replication in the tracheal tissue of broilers. Clinical symptoms and histopathological results show that forsythoside can significantly alleviate IBV infection-induced slow weight gain, respiratory distress, and tracheal tissue damage in broilers. This invention demonstrates that forsythoside has low cytotoxicity, can effectively prevent infectious bronchitis in chickens, and has advantages such as being green and safe, leaving no residue, having few toxic side effects, and being unlikely to induce drug resistance.