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Method for determining contents of seven components in lonicera and forsythia powder by adopting dual-wavelength quantitative analysis of multi-components by single marker
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A measurement method and dual-wavelength technology, applied in measuring devices, material separation, and analysis of materials, can solve problems such as inability to comprehensively evaluate and single components
Pending Publication Date: 2021-07-06
SHANDONG ACAD OF CHINESE MEDICINE
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[0004] Aiming at the problems existing in the prior art that there is only a single quality control index component for detecting Yinqiao powder, and it is impossible to comprehensively evaluate honeysuckle and forsythia as the monarch drug ingredients in the prescription, the present invention provides a dual-wavelength one-measurement-multiple-evaluation The method for determining the content of 7 components in Yinqiao powder by using the method of one test and multiple evaluations, using forsythiaside A as the internal reference, and establishing the relationship between this component and forsythin, arctiin, neochlorogenic acid, chlorogenic acid, and isochlorogenic acid Relative correction factors of chlorogenic acid A and isochlorogenic acid C to obtain the content of 7 components in Yinqiao powder
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Embodiment 1
[0036] (1) Preparation of reference solution: Accurately weigh neochlorogenic acid, chlorogenic acid, forsythiaside A, isochlorogenic acid A, isochlorogenic acid C, forsythin, and arctiin reference substances, and use 50% The chromatographic methanol is configured as a mixed reference solution with a concentration of 7.7, 96, 296, 65, 15.2, 27.5, and 113 μg / ml;
[0037] (2) Preparation of the test solution: Accurately weigh 0.5g of Yinqiaosan, dilute to volume with 50% methanolaqueous solution, weigh, ultrasonicate for 30 minutes, let cool, make up for weight loss, filter, and take the filtrate to pass through 0.45μm organic micropores Filter the sample solution to be tested, and the concentration is 0.02g / ml; prepare negative samples lacking honeysuckle, forsythia, arctiin single medicinal material, and negative samples lacking honeysuckle and Arctium two medicinal materials respectively in the same way;
[0067] Take 10 batches of Yinqiao powder samples, prepare the test solution according to the method of step (2) of embodiment 1, inject samples according to the chromatographic conditions of step (3) of the embodiment, and record at 237nm, forsythiaside A, forsythin, Peak area of arctiin, peak area of forsythiaside A, neochlorogenic acid, chlorogenic acid (C), isochlorogenic acid A, and isochlorogenic acid C at 327nm.
[0068] Using the relative correction factor in Example 1, the external standard one-point method (ESM) and the one-measurement-multiple-evaluation method (QAMS) were used to calculate the content of each component of Yinqiao powder. The relative deviation of the results of the two methods of ESM and QAMS was -0.35 % to 0.11%, all less than 3% (see Table 6 and Table 7).
[0071] Table 7 Comparison of ESM and QAMS calculation results at 327nm
[0072] .
[00...
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Abstract
The invention discloses a method for determining the contents of seven components in lonicera and forsythiapowder by adopting dual-wavelength quantitative analysis of multi-components by a single marker, which comprises the following steps of: respectively taking neochlorogenic acid, chlorogenic acid, forsythiaside A, isochlorogenic acid A, isochlorogenic acid C, forsythin and arctiin as reference substances and forsythiaside A as an internal reference substance, calculating the relative correction factors of arctiin and forsythin at 237 nm, calculating the relative correction factors of neochlorogenic acid, chlorogenic acid, isochlorogenic acid A and isochlorogenic acid C at 327 nm, taking a forsythiaside A reference substance solution and a test solution, injecting the solutions into a high performance liquid chromatograph, and calculating the contents of seven components including forsythiaside A, forsythin, arctiin, neochlorogenic acid, chlorogenic acid, isochlorogenic acid A and isochlorogenic acid C in the lonicera and forsythiapowder according to the relative correction factors. According to the invention, the same test solution is adopted to determine the contents of seven components under two absorption wavelengths, so that the detection efficiency is improved, and the detection cost is reduced.
Description
technical field [0001] The invention belongs to the technical field of quality control and analysis of traditional Chinese medicines, and in particular relates to a method for measuring the contents of seven components in Yinqiao Powder by using a dual-wavelength one-measurement and multiple-evaluation method. Background technique [0002] The quality of traditional Chinese medicine is the premise to ensure the effectiveness and safety of clinical medication, the key factor to ensure the efficacy of traditional Chinese medicine, and the basic guarantee for the modernization and scientificization of traditional Chinese medicine. Therefore, improving the quality control standard of traditional Chinese medicine is particularly important for improving the quality of traditional Chinese medicine. Traditional Chinese medicine compound prescriptions are holistic and systematic in clinical application, but using a single component to evaluate the quality of traditional Chinese medici...
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