36 results about "Isochlorogenic acid" patented technology

This invention belongs to the field of traditional Chinese medicine preparation analysis, specifically disclosing a one-test-multiple-evaluation method for determining the content of seven components in Jinteng Qingbi granules and its application. Using sinomenine as an internal standard, a one-test-multiple-evaluation method is used to establish relative correction factors between sinomenine and gallic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, and isochlorogenic acid C in Jinteng Qingbi granules. The contents of gallic acid, neochlorogenic acid, sinomenine, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, and isochlorogenic acid C in Jinteng Qingbi granules are calculated using these correction factors. This invention's method is highly practical, simple to operate, cost-effective, and provides accurate and reliable test results, overcoming the shortcomings of existing methods for quality testing of Jinteng Qingbi granules and providing a new approach for quality control and intrinsic quality evaluation of Jinteng Qingbi granules.

The application relates to the field of traditional Chinese medicine quality control, and particularly discloses a quality control method for Shuanghuanglian inhalation solution. The method can simultaneously determine the fingerprint spectrum and ingredient content of the Shuanghuanglian inhalation solution. The fingerprint spectrum detection method disclosed by the application adopts UPLC determination, has 17 characteristic peaks, and attributes 8 characteristic peaks. The contents of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C and forsythoside are simultaneously determined. Compared with the prior art, the method for determining the fingerprint spectrum and content of the Shuanghuanglian inhalation solution is improved, and the quality of the Shuanghuanglian inhalation solution can be comprehensively monitored conveniently, quickly and accurately.

The invention belongs to the technical field of medicinal material quality control, and discloses a honeysuckle flower QAMS (quantitative analysis of multi-components by single marker) quality control method, which comprises the following steps: preparing a single reference solution and a mixed reference solution of chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A and isochlorogenic acid C, and preparing a test solution; after high performance liquid chromatography detection, the relative correction factors of other components in the honeysuckle are calculated by taking chlorogenic acid as an internal reference substance through chromatographic peak positioning, and the contents of the components in the honeysuckle are obtained by utilizing the relative correction components, so that the synchronous determination of the multiple components in the honeysuckle is realized. The method has the advantages of cost saving, simplicity in operation, high detection accuracy and good stability, has great significance in quality control and clinical curative effect guarantee of the honeysuckle medicinal material, and provides a basis for establishing a quality evaluation standard of the honeysuckle medicinal material.

The invention discloses a method for simultaneously preparing high-content isochlorogenic acid A and high-content isochlorogenic acid C. The method comprises the following steps: dissolving a stevia rebaudiana polyphenol product in purified water, and adjusting the pH value to obtain a treating fluid 1; adsorbing the treating fluid 1 through macroporous adsorption resin, jacking the treating fluid 1 through purified water, and mixing effluent liquid during adsorption and purified water jacking to obtain effluent liquid 1; concentrating the effluent 1 by using a reverse osmosis membrane, adjusting the pH value to obtain a treating fluid 2, and extracting the treating fluid 2 by using an ethyl acetate saturated aqueous solution to obtain a raffinate phase I and an extract phase I; adjusting the pH value of the raffinate phase I, and extracting with an ethyl acetate-n-butyl alcohol saturated aqueous solution to obtain a raffinate phase II and an extract phase II; after drying the extract phase I, sequentially crystallizing and recrystallizing to obtain high-purity isochlorogenic acid C; and drying the extract phase II, and sequentially crystallizing, pulping and purifying to obtain the high-purity isochlorogenic acid A. The method comprises the following steps: removing impurities by adopting macroporous adsorption resin, extracting by adopting a solvent step by step, carrying out crude separation on the isochlorogenic acid A and the isochlorogenic acid C, and carrying out subsequent crystallization and refining to obtain the high-content isochlorogenic acid A and the high-content isochlorogenic acid C.

This invention discloses a method for extracting chlorogenic acids from stevia waste and its applications, including: extracting chlorogenic acids from stevia powder using an alkaline extraction method; and determining whether the chlorogenic acids contain chlorogenic acid, isochlorogenic acid A, and cryptochlorogenic acid. Using stevia waste as raw material, this invention involves solvent extraction, centrifugal filtration, reflux extraction, and chromatographic separation to obtain a fat-soluble extract. This extract is then dissolved and filtered with ethanol, evaporated and concentrated, and freeze-dried to obtain the chlorogenic acid product. This invention extracts and develops chlorogenic acids from stevia waste, solving the problems of environmental pollution and resource waste. The extracted chlorogenic acids contain chlorogenic acid, cryptochlorogenic acid, and isochlorogenic acid A, and have low sugar content. They possess antioxidant, anti-inflammatory, and blood pressure-lowering functions, and can be widely used in food, pharmaceuticals, and health products, demonstrating high application value and realizing the comprehensive utilization of stevia.

The invention belongs to the technical field of traditional Chinese medicine component detection and analysis, and particularly relates to a method for detecting various active components in lonicerae flos by quantitative analysis of multi-components by single marker. According to the QAMS detection method for the various active ingredients in the lonicera confusa, based on a simple, convenient and rapid high performance liquid chromatography technology, QAMS of the active ingredients such as flavonoids, flavonoid glycosides, iridoid glycosides and organic acids in the lonicera confusa can be achieved, and qualitative and quantitative detection of the various active ingredients is included. The detection target objects comprise 14 active components, namely neochlorogenic acid, cumaric acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, swertiamarin, secologanin, rutin, quercitrin, galuteolin, ferulic acid, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C. The invention further provides a method for detecting the content of the isochlorogenic acid. Tests prove that the method disclosed by the invention meets methodological requirements, has the characteristics of simplicity and convenience in operation, rapidness in detection, high sensitivity, accuracy in determination and strong applicability, is beneficial to accurately evaluating the quality of the lonicerae flos and provides a reliable basis for safe application of the lonicerae flos.

The present application relates to the technical field of pharmaceutical compositions, and provides a composition for improving immune function, a preparation and a preparation method and application thereof, comprising: 25-40% of chlorogenic acid, 5-12% of luteolin, 10-20% of isochlorogenic acid A, 3-8% of caffeic acid, 0.5-1% of a targeting ligand, 2-5% of a microecological component, and 5-8% of a nano-carrier, and application of the composition in preparation of medicines, health products and functional foods for improving immune function, and a preparation method of the above-mentioned medicine preparation, comprising the following steps: preparing a core particle; coating the core particle with a sustained-release enteric coating to obtain a microcapsule particle; filling the microcapsule particle into a capsule shell to obtain an enteric capsule; coating the enteric capsule with a stomach-dissolving coating liquid to obtain a capsule preparation; and achieving the effect of improving the whole gastrointestinal immune function by rapidly targeting anti-inflammation in the stomach and continuously targeting regulation in the intestinal tract, and greatly improving the action efficiency and immune regulation effect of the preparation.

The present application relates to a kind of methods for screening anti-inflammatory, antioxidant active compounds from natural Chinese herbal medicine wormwood based on spectrum-effect relationship, the method establishes the method for the common characteristic peak of the fingerprint chromatogram of wormwood extract ultra-high performance liquid chromatography.By grey correlation analysis, Pearson bivariate correlation analysis method and partial least square regression analysis etc. chemometrics method establishes the spectrum-effect relationship of wormwood anti-inflammatory, antioxidant activity, to screen the anti-inflammatory, antioxidant active compounds in wormwood.The analysis result shows that compound chlorogenic acid, isochlorogenic acid A, isochlorogenic acid C is the main anti-inflammatory, antioxidant active compound in wormwood extract.The present application provides scientific and effective method for the research of the medicinal material basis of wormwood and quality control, and provides reference for the development and utilization of wormwood.

The application relates to the field of biological medicine, and discloses self-assembled nanoparticles and a preparation method and application thereof. The self-assembled nanoparticles are formed by self-assembly of isoeuropetin and isochlorogenic acid C through non-covalent bond interaction to form supramolecules, and the particle size of the self-assembled nanoparticles is 50-200 nm, and the Zeta potential is-14.8 mV to-35.2 mV. Based on the self-assembly characteristics of active small molecules of traditional Chinese medicine, it is found and confirmed that EUP and IAC can be self-assembled to form stable nano drugs (EUP-IACs) through various technical means. The nano drugs significantly enhance the inhibiting effect on MRSA USA 300 through the synergistic effect of EUP and IAC, and the application provides a new idea and strategy for optimizing the preparation of traditional Chinese medicine carrier-free self-assembled nano and expanding the application of the traditional Chinese medicine in the antibacterial field.

The invention provides a method for detecting HPLC (high performance liquid chromatography) characteristic chromatograms of senecio scandens medicinal materials, decoction pieces, standard decoction and formula granules, which comprises the following steps: pre-treating raw materials of a test sample to obtain liquid to be detected; the pretreatment mode comprises solvent dissolution and extraction; measuring the liquid to be measured by adopting HPLC (High Performance Liquid Chromatography) to obtain HPLC characteristic chromatograms of the senecio scandens medicinal material, decoction pieces, standard decoction and formula granules; the preparation method comprises the following steps: preparing a reference substance solution, namely dissolving neochlorogenic acid, protocatechuic aldehyde, chlorogenic acid, caffeic acid, cryptochlorogenic acid, p-coumaric acid, chlorogenic acid methyl ester, rutin, hyperoside, isoquercitrin, chlorogenic acid ethyl ester, isorhamnetin-3-O-galactoside, quercitrin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, quercetin and kaempferol by adopting a solvent; obtaining a reference substance solution of the reference substance; the HPLC conditions are as follows: a chromatographic column is a phenyl column; a mobile phase A is an acetonitrile solution, a mobile phase B is a 0.3% phosphoric acid aqueous solution, and gradient elution is carried out; according to the method, a high performance liquid chromatography method is adopted, and an acetonitrile-0. 3% phosphoric acid solution is selected as a mobile phase for gradient elution; the method comprises the following steps: taking neochlorogenic acid, protocatechuic aldehyde, chlorogenic acid, caffeic acid, cryptochlorogenic acid, p-coumaric acid, chlorogenic acid methyl ester, rutin, hyperoside, isoquercitrin, chlorogenic acid ethyl ester, isorhamnetin-3-O-galactoside, quercitrin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, quercetin and kaempferol as reference substances; the HPLC characteristic chromatogram method of the senecio scandens medicinal material, the decoction pieces, the standard decoction and the formula granules is established, and more scientific technical means are provided for controlling the medicinal quality of the senecio scandens medicinal material, the decoction pieces, the standard decoction and the formula granules.

The present disclosure discloses a method for constructing a chicory poria complex fingerprint, including (1) preparing a sample solution of a chicory poria complex, (2) preparing a mixed control solution using chlorogenic acid, 6'-O-β-D-glucosylgentiopicroside, swerthiamarin, gentiopicroside, swelloside, 3-O-caffeoylshikimic acid, neoastilbin, astilbin, rutin, isoastilbin, isochlorogenic acid C, quercitrin, kaempferol-3-O-rutinoside, and narcissine, and (3) injecting the sample solution and the mixed control solution into a high-performance liquid chromatograph, respectively, and performing a similarity analysis on the resulting chromatograms to obtain a chicory poria complex fingerprint. The present disclosure also provides a chicory poria complex fingerprint constructed by the construction method and its application to identifying and / or evaluating the quality of chicory poria complexes. The disclosed chicory poria compound fingerprint can comprehensively, objectively, and accurately detect and evaluate the quality of chicory poria compound, thereby ensuring safe and effective medication for patients.

The application belongs to the field of traditional Chinese medicine quality analysis and control, and discloses a determination method of ingredients content of scabiosa comosa, which takes isochlorogenic acid A as an internal reference, calculates the relative correction factors of chlorogenic acid, caffeic acid, jinyingzhi, 11(13)-dehydroxy axillary ivy chrysanthemum, isochlorogenic acid C, 2,3,4,5-tetra caffeoyl-D-glucaric acid, 2-deoxy-4-tablet-Tianrenjuling, scabioside lactone ketone and terlene lactone, quickly and accurately detects the content of 10 kinds of ingredients of scabiosa comosa, and solves the problems of many required control products, difficulty in obtaining and high price in the determination of scabiosa comosa content, and high detection cost.

The invention provides a method for extracting isochlorogenic acid from plant processing by-products, and particularly belongs to the technical field of plant by-product utilization. The method comprises the following steps: dissolving the plant processing by-product by adopting a first short-chain alcohol solvent to obtain a first mixed solution; mixing the first mixed solution with a non-polar organic extraction solvent, and extracting to obtain an alcohol-water phase; precipitating the alcohol-water phase by adopting a precipitation reagent to obtain an isochlorogenic acid precipitate; dissolving the isochlorogenic acid precipitate in a second short-chain alcohol solvent, mixing with acid, carrying out double decomposition reaction, and carrying out solid-liquid separation to obtain a first filtrate; adjusting the pH value to 2-4, removing short-chain alcohol in the first filtrate, and carrying out solid-liquid separation to obtain an isochlorogenic acid precipitate crude product; and dissolving the isochlorogenic acid precipitate crude product in a third short-chain alcohol solvent, and recrystallizing to obtain the isochlorogenic acid. The method provided by the invention can realize comprehensive utilization of plant processing byproducts, and is simple in process, easy to operate and suitable for large-scale industrial production.

The invention belongs to the technical field of analysis and detection, and provides a method for identifying astringent phenolic acids in coffee and application of the method, phenolic acid compounds in coffee are qualitatively and quantitatively determined through an LC-MS / MS technology, and astringent compounds with the greatest contribution in a coffee sample are determined according to a taste recognition threshold concentration so as to identify key astringent phenolic acids. On the basis, the interaction mechanism of key astringent phenolic acids with different structures and concentrations and salivary protein models is analyzed through binding constants, binding sites, interaction force and thermodynamic parameters. The astringent taste intensity of phenolic acid is evaluated by turbidity, particle size and Zeta potential, the structure-effect and dose-effect relationship between phenolic acid and astringent taste intensity is established, and an application basis is provided for regulation and control of astringent taste of coffee. Results show that in astringent phenolic acid, the astringent perception threshold value of isochlorogenic acid B is lowest, the Dot value is highest, the astringent ability of isochlorogenic acid B is highest, and the content of isochlorogenic acid B needs to be reduced or the reaction of isochlorogenic acid B and sialoprotein needs to be inhibited when astringent of coffee is regulated.

This invention discloses a method for constructing a fingerprint spectrum of a throat-clearing hard candy and a method for detecting its component content, belonging to the field of component detection technology. In this invention, the throat-clearing hard candy sample solution is analyzed by high-performance liquid chromatography (HPLC) to obtain a fingerprint spectrum of the candy; and the content information of the components is obtained through the external standard method, realizing qualitative and quantitative analysis of the components of the throat-clearing hard candy. Specifically, it involves the simultaneous qualitative and quantitative analysis of the following 10 components: gallic acid, neochlorogenic acid, chlorogenic acid, isorhamnetin-3-O-glucose-7-O-rhamnoside, rutin, luteolin, isochlorogenic acid B, isorhamnetin-3-O-glucose, glycyrrhizic acid, and kaempferol, providing a reliable and quantifiable standard for the quality assessment of throat-clearing hard candy. The chromatogram obtained by the method of this invention has the characteristics of stable baseline, high peak resolution, strong specificity, and good peak symmetry.

The invention discloses a method for extracting isochlorogenic acid C from folium artemisiae argyi. The method comprises the following steps: S1, crushing dried folium artemisiae argyi to 20-80 meshes to obtain folium artemisiae argyi powder; s2, carrying out synergistic wall breaking with a deep eutectic solvent-enzyme composite wall breaking solution system according to a solid-liquid ratio of 1: 10-1: 30 g / mL to obtain a wall-broken mixture; s3, adding a magnetic molecularly imprinted polymer Fe3O4 (at) IsoC-MIP to selectively adsorb the isochlorogenic acid C, and separating by a permanent magnet to obtain magnetic particles loaded with the isochlorogenic acid C; and S4, desorbing with an eluent, concentrating under reduced pressure, and freeze-drying to obtain a high-purity product. According to the extraction method, the low-temperature wall-breaking energy consumption is reduced, the solvent dosage is small, the isochlorogenic acid C with HPLC larger than or equal to 95% and the recovery rate larger than or equal to 92% can be obtained through rapid magnetic separation, magnetic particles can be cycled for 20 times, no toxic residues exist, and green, efficient, low-cost and large-scale extraction is achieved.

The application discloses a kind of construction methods of Jujing compound fingerprint, comprising: (1) Jujing compound is made into test solution;(2) using chlorogenic acid, 6'-O-β-D-glucosyl gentiopicroside, swertiamarin, gentiopicroside, swertisin, 3-O-coffeicoyl shikimic acid, astilbin, astilbinin, rutin, isofraxin, isochlorogenic acid C, quercitrin, kaempferol-3-O-rutinoside and zephyranthine preparation mixed reference solution;(3) respectively test solution and mixed reference solution are injected into high performance liquid chromatograph, the similarity analysis is carried out to the obtained chromatogram, and Jujing compound fingerprint is obtained.The application also provides Jujing compound fingerprint obtained by the construction method and its application in identifying and / or evaluating the quality of Jujing compound.The Jujing compound fingerprint of the application can comprehensively, objectively and accurately detect and evaluate the quality of Jujing compound, so as to ensure the safety and effectiveness of patient medication.

The invention discloses a method for improving curcumin embedding capacity of myofibrillar protein, and belongs to the technical field of food processing. The method for improving curcumin embedding capacity of myofibrillar protein comprises the following steps: dissolving myofibrillar protein powder in a low-salt solution, and carrying out ultrasonication to obtain a protein solution; adding an enzyme into the protein solution, then reacting, and deactivating the enzyme to obtain a mixed solution; adjusting the pH value of the mixed solution to 11-13, adding 4, 5-dicaffeoylquinic acid into the mixed solution, and then reacting in a dark place to obtain the isochlorogenic acid C-ultrasonic-assisted enzyme treated myofibrillar protein compound. According to the method, ultrasonic-enzyme and 4, 5-DCQA treatment is combined, so that the structure of the myofibrillar protein is effectively improved, and the curcumin embedding capacity of the myofibrillar protein is remarkably improved.

The invention relates to the technical field of drug therapy, and discloses application of isochlorogenic acid A. Animal experiments and cell experiments prove that the isochlorogenic acid A can reduce the in-vivo inflammation level of cells and POI mice, improve the uterus and ovarian indexes of the POI mice, reverse ovarian follicle injury and improve the curative effect of the POI mice on early-onset ovarian insufficiency. According to the invention, the number of granular cells is increased, the proportion of atresia follicles is reduced, and chronic ovarian injury caused by microenvironment or POI caused by chemotherapy can be reversed under the dosage of 5-10mg / kg. The compound is wide in source, high in safety and excellent in treatment effect.

The present application relates to the technical field of pharmaceutical chemistry, in particular to a preparation method and application of a berberine and isovitexin nanomedicine. The application is used in the preparation of a drug for treating diseases related to inflammation mediated by neutrophils. The berberine and isovitexin or the structural analogues of berberine and isovitexin form nanoparticles in a carrier-free form through self-assembly. The berberine and isovitexin nanomedicine has a synergistic anti-inflammatory and antioxidant effect, can efficiently inhibit key inflammatory mediators, realize high-efficiency anti-inflammatory and antioxidant synergy, and significantly improve the effect of preventing and treating acute inflammation.

The invention discloses a preparation method of high-purity isochlorogenic acid B. The preparation method specifically comprises the following steps: dissolving a stevia rebaudiana polyphenol product, then adjusting the pH value to obtain a to-be-treated liquid I, heating to carry out isomerization reaction, after the reaction is finished, adjusting the pH value, concentrating, and cooling to obtain a to-be-treated liquid II; extracting the to-be-treated liquid II for multiple times to obtain a raffinate phase I, and adjusting the pH value of the raffinate phase I to obtain to-be-treated liquid III; extracting the to-be-treated liquid III for multiple times by adopting a water-saturated ethyl acetate solution to obtain a raffinate phase II; adsorbing the raffinate phase II through non-polar macroporous adsorption resin, sequentially carrying out jacking and desorption treatment, adjusting the pH value of a desorption solution, and drying to obtain a crude product; and carrying out crystallization and recrystallization treatment on the crude product to obtain the high-purity isochlorogenic acid B. According to the method, a stevia rebaudiana polyphenol product is used as a raw material, high-purity isochlorogenic acid B is prepared through isomerization reaction, extraction, resin adsorption and fractional crystallization, waste liquid generated in the process is fully utilized, the resource utilization rate is increased, and the product yield is increased.

This invention belongs to the field of tea production fermentation technology, specifically disclosing a *Fungi* strain for improving tea quality and its application, namely *Fungi* strain Fungi37, classified and named... Diaspora masirevicii The strain, with accession number CGMCC No. 42364, is a rare endophytic fungus found in tea plants and is the first reported strain in the field of tea. Experiments have shown that this strain can significantly enhance the brightness and vividness of tea infusion and play a certain regulatory role in the flavor presentation of tea. Metabolomics analysis shows that this bacterium can promote the large-scale conversion and synthesis of active ingredients such as methyl caffeate, methyl gallate, ethyl gallate, hesperidin, and isosulfanol during tea fermentation. Furthermore, in an exogenous sugar-added environment, it can promote the conversion and synthesis of beneficial components such as dihydromyricetin, yarrow aglycone, isochlorogenic acid A, plantainin, malic acid, sorbic acid, and gluconic acid.

The invention provides a method for preparing neochlorogenic acid salt and a method for preparing neochlorogenic acid, and particularly belongs to the technical field of neochlorogenic acid synthesis. The method for preparing the neochlorogenic acid salt comprises the following steps: mixing isochlorogenic acid salt with a short-chain alcohol solvent, and carrying out alcoholysis reaction at the temperature of 40-65 DEG C under an alkaline condition to generate the neochlorogenic acid salt, the isochlorogenic acid salt is 3, 5-dicaffeoylquinic acid salt and / or 4, 5-dicaffeoylquinic acid salt. According to the method disclosed by the invention, the isochlorogenic acid can be used as a raw material to prepare the neochlorogenic acid through alcoholysis, and the raw material for preparing the isochlorogenic acid can be isochlorogenic acid or isochlorogenic acid separated from plant byproducts. The method is simple in process, high in product yield and suitable for large-scale industrial production, low-valued plant byproducts can be utilized in a high-valued mode, isochlorogenic acid can be used as a raw material, and efficient preparation of neochlorogenic acid is achieved.

This invention belongs to the field of traditional Chinese medicine processing and natural product chemistry, specifically relating to a method for the targeted enrichment of chlorogenic acid components in honeysuckle. The method involves placing honeysuckle powder under constant temperature heating conditions for dry heat treatment. Different dry heat treatment conditions are set according to different target concentrators to achieve the targeted enrichment of various chlorogenic acid components, including neochlorogenic acid, chlorogenic acid, isochlorogenic acid B, and isochlorogenic acid C. This method achieves the targeted and efficient enrichment of effective components during the processing of honeysuckle tablets, and the process is simple, convenient to operate, and easy to scale up for industrial production.

The invention belongs to the technical field of biological medicines, and discloses application of isochlorogenic acid B in preparation of a medicine for preventing and treating coronavirus pneumonia. It is known that hnRNPA2B1 can specifically recognize virus genetic materials, and the natural immune response to viruses is started. Experiments prove that the isochlorogenic acid B can be combined with hnRNPA2B1 host protein to mediate the virus infection process, the antiviral immune response is affected, lung tissue damage caused by coronavirus infection is effectively relieved, the virus load in lung tissue is reduced, the effect of preventing and treating coronavirus pneumonia is achieved, and the isochlorogenic acid B is good in safety as a natural product.

This invention discloses a method for determining the quality of *Hemiberlesia lingua* stems and leaves. The method includes fingerprint spectroscopy establishment and similarity analysis, content determination, cluster analysis, principal component analysis, and partial least squares discriminant analysis. The method of this invention involves UPLC fingerprint analysis and similarity analysis of 15 batches of *Hemiberlesia lingua* stems and leaves from different origins. A total of 28 common peaks were found, and 7 of these common peaks were identified using reference standards: peak 4 (neochlorogenic acid), peak 8 (chlorogenic acid), peak 9 (caffeic acid), peak 11 (cryptochlorogenic acid), peak 22 (isoquercetin), peak 24 (astragalin), and peak 28 (isochlorogenic acid C). This invention's method is highly sensitive, specific, stable, and feasible, providing a reference for the quality evaluation and control of *Hemiberlesia lingua* stems and leaves.

This invention provides a dietary fiber-isochlorogenic acid complex, its preparation method, and its applications, relating to the field of food processing technology. The invention employs an ultrasound-assisted eutectic solvent method to extract soluble dietary fiber from the outer leaves of Chinese cabbage. The hydrogen bond donor in the eutectic solvent is lactic acid, and the hydrogen bond acceptor is one of choline chloride, glycine, and alanine. Simultaneously, this invention prepares a soluble dietary fiber-stevia isochlorogenic acid complex from the outer leaves of Chinese cabbage and investigates its protective effect on the intestinal barrier in mice. The results show that the soluble dietary fiber-stevia isochlorogenic acid complex from the outer leaves of Chinese cabbage can protect the integrity of mouse intestinal villi, reduce intestinal barrier permeability, maintain the function of mouse immune organs and the intestinal immune barrier, maintain the integrity of the intestinal mechanical barrier, improve the intestinal flora structure, and enhance humoral immunity, cellular immunity, and non-specific immune function.

The invention belongs to the technical field of tea production and fermentation, and particularly discloses a phlomis spp. Strain capable of improving tea quality and application of the phlomis spp. Strain, the classification name of the phlomis spp. Strain is Diaporthe masrevicii, and the preservation number of the phlomis spp. Strain is CGMCC No.42364. The strain is reported for the first time in the field of tea and belongs to a rare tea tree endophyte. Experiments prove that the strain can significantly improve the brightness and the brilliance degree of tea soup, and has a certain adjusting effect on the taste of the tea soup; metabonomics analysis shows that the strain can promote mass conversion and synthesis of functional components such as methyl caffeate, methyl gallate, ethyl gallate, hesperidin, isoschaftoside and the like in a tea soup fermentation process; the conversion and synthesis of beneficial components such as dihydromyricetin, leucocyanidin, isochlorogenic acid A, plantaginin, malic acid, sorbic acid and gluconic acid can be promoted in an exogenous sugar addition environment.

Disclosed is an industrial method for simultaneously preparingStevia rebaudiana chlorogenic acid and stevioside. The industrial method includes carrying out alcohol extraction on Stevia rebaudiana which is used as a raw material, and then adjusting the feed liquid state to allow chlorogenic acid to be in a free molecular state; carrying out extraction separation by a water-insoluble moderate-polarity organic solvent; enriching the Stevia rebaudiana chlorogenic acid in an organic layer; and enriching the stevioside in a water layer. Compared with a traditional water extraction process, the method has the advantages that chlorogenic acid ingredient in the Stevia rebaudiana can be prevented from being hydrolyzed, such that the contents and effects of effective ingredients in Stevia rebaudiana chlorogenic acid products can be guaranteed; effective separation can be carried out on the premise that the quality and the production efficiency of stevioside products are unaffected, the production efficiency can be improved, and the ratio of isochlorogenic acid to total chlorogenic acid in the resulting products is close to that in the raw material; production water consumption can be reduced, and discharge of sewage and flocculation residues can be decreased; and accordingly, the method is a green production process with high benefits.

The invention discloses a method for jointly separating isochlorogenic acid A and isochlorogenic acid C based on extraction and chromatography. The method comprises the following steps: preparing a stevia rebaudiana polyphenol solution, and adjusting the pH value of the stevia rebaudiana polyphenol solution to obtain a first treatment solution; extracting the first treating fluid by using an ethyl acetate saturated aqueous solution, collecting a raffinate phase and an extract phase, drying the raffinate phase, dissolving the raffinate phase in a methanol solution, and filtering to obtain a second treating fluid; separating the second treatment liquid in a chromatographic column; sequentially eluting by adopting a 92-94% v / v methanol solution, a 85-87% v / v methanol solution, a 78-80% v / v methanol solution and a 65-70% v / v methanol solution, and respectively collecting a first eluant, a second eluant, a third eluant and a fourth eluant; drying the second eluent, and sequentially crystallizing and recrystallizing to obtain high-purity isochlorogenic acid C; and drying the fourth eluent, and sequentially crystallizing and recrystallizing to obtain the high-purity isochlorogenic acid A. According to the method, high-content isochlorogenic acid A and isochlorogenic acid C are recovered from a stevia rebaudiana polyphenol product through extraction, chromatographic separation and crystallization, and the resource utilization rate is increased.