52 results about "Isochlorogenic acid" patented technology

The invention provides a folium ilicis cornutae extract as well as a preparation method and application thereof. The core component of the holly leaf extract comprises polyphenol compounds, the total polyphenol content is 10-500mg / g, and the polyphenol components comprise neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, isoquercitrin, isochlorogenic acid B, isochlorogenic acid C and isochlorogenic acid A according to a ratio of (95-100): (300-450): (70-109): (120-256): (20-46): (21-156): (24-43): (27-61). The preparation method of the Chinese holly leaf extract comprises the whole processes of raw material pretreatment, multi-solvent synergistic extraction, multi-stage double-effect filtration and impurity removal, deep concentration and drying, parameters of all links are accurately controlled, the method is high in extraction rate, and the obtained Chinese holly leaf extract can effectively reduce the uric acid level and prevent and treat hyperuricemia.

The invention provides a honeysuckle MYB gene family transcription factor 13476. A honeysuckle HQT2 gene, an HQT2 gene promoter and a transcription factor 13476 are cloned respectively, and a yeast one-hybrid experiment proves that 13476 can directly interact with the HQT2 promoter. Furthermore, the regulation of the 13476 protein on the transcriptional activity of the HQT2 gene is analyzed by using a dual luciferase system in tobacco, and the 13476 is proved to have an inhibiting effect on the promoter of the HQT2. Tobacco transient transformation results show that the content of chlorogenic acid in plant cells can be increased by 30-40% through heterologous transient expression of the LjHQT2 gene, and the content of chlorogenic acid reaches the level of 1.59 mg / g. The RNAi method is adopted, molecules aiming at Lj13476 are transiently expressed in honeysuckle, the content of chlorogenic acid can be increased by 10%-20%, the content of isochlorogenic acid A can be increased by 5%-30%, and the content of isochlorogenic acid C can be increased by 7%-20%. The interaction mechanism of the honeysuckle HQT2 gene and the transcription factor 13476 is studied, a certain basis is provided for the regulation mechanism of chlorogenic acid, and the HQT2 gene and the transcription factor 13476 are of great significance for breeding high-quality honeysuckle in the future.

This invention belongs to the field of traditional Chinese medicine preparation analysis, specifically disclosing a one-test-multiple-evaluation method for determining the content of seven components in Jinteng Qingbi granules and its application. Using sinomenine as an internal standard, a one-test-multiple-evaluation method is used to establish relative correction factors between sinomenine and gallic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, and isochlorogenic acid C in Jinteng Qingbi granules. The contents of gallic acid, neochlorogenic acid, sinomenine, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, and isochlorogenic acid C in Jinteng Qingbi granules are calculated using these correction factors. This invention's method is highly practical, simple to operate, cost-effective, and provides accurate and reliable test results, overcoming the shortcomings of existing methods for quality testing of Jinteng Qingbi granules and providing a new approach for quality control and intrinsic quality evaluation of Jinteng Qingbi granules.

The application relates to the field of traditional Chinese medicine quality control, and particularly discloses a quality control method for Shuanghuanglian inhalation solution. The method can simultaneously determine the fingerprint spectrum and ingredient content of the Shuanghuanglian inhalation solution. The fingerprint spectrum detection method disclosed by the application adopts UPLC determination, has 17 characteristic peaks, and attributes 8 characteristic peaks. The contents of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C and forsythoside are simultaneously determined. Compared with the prior art, the method for determining the fingerprint spectrum and content of the Shuanghuanglian inhalation solution is improved, and the quality of the Shuanghuanglian inhalation solution can be comprehensively monitored conveniently, quickly and accurately.

The invention relates to a characteristic marker combination for identifying honeysuckle tea varieties and an identification method thereof, and belongs to the technical field of variety tea identification. The characteristic marker combination comprises chlorogenic acid, swertioside, secoxyloganin, galuteolin, isochlorogenic acid A and isochlorogenic acid C. The invention further discloses a kit for detecting the secoxyloganin. The contents of the characteristic markers are simultaneously detected through a high performance liquid chromatography (HPLC) or liquid chromatography-mass spectrometry (LC-MS) technology, and different honeysuckle tea varieties can be rapidly and accurately identified by combining multivariate statistical analysis (such as principal component analysis or clustering analysis). The identification method provided by the invention has the characteristics of strong specificity, high stability and high sensitivity, is suitable for large-scale sample analysis, and can be widely applied to quality control, authenticity identification and market supervision of honeysuckle tea varieties.

The invention belongs to the technical field of medicine technology, particularly relates to application of isochlorogenic acid C in medicine for treating inflammation, and provides novel application of isochlorogenic acid C in preparation of anti-inflammation medicine, and particularly has a good inhibition effect on abnormal behaviors such as invasion of synovial fibroblasts and secretion of inflammatory factors. Isochlorogenic acid C is provided as an effective medicine component for treating arthritis, and the main action mechanism is to inhibit invasion of RA-FLS and secretion of IL-6 so as to relieve clinical symptoms; the innovation of the invention lies in that: the invention provides treatment of inflammation based on isochlorogenic acid C, and through application of a specific dosage, joint inflammation of patients can be effectively relieved and joint functions can be improved.

The invention discloses a method for continuously preparing neochlorogenic acid through a two-phase system, and belongs to the technical field of plant byproduct utilization. The method comprises the following steps: dissolving a raw material containing isochlorogenic acid in ethyl acetate, adding a proper amount of water, adding alkali to adjust the pH value to 7-11, and carrying out a first hydrolysis reaction in a water-ethyl acetate two-phase system reaction solution; after the first hydrolysis reaction is finished, adjusting the pH value of a reaction liquid water layer to 2-6, and separating the water layer from an ethyl acetate layer; adding water into the ethyl acetate layer, and continuing to repeat the hydrolysis reaction; collecting and combining water layers separated each time, performing macroporous adsorption resin purification and methanol desorption on the water layers, collecting desorption liquid, concentrating and spraying to obtain the neochlorogenic acid. The total content of chlorogenic acid in the prepared product can reach 75% and above, and the content of neochlorogenic acid 5-CQA can reach 50% and above. The method has the advantages of simple process, easiness in operation and the like, and is suitable for large-scale industrial production.

The invention belongs to the technical field of medicinal material quality control, and discloses a honeysuckle flower QAMS (quantitative analysis of multi-components by single marker) quality control method, which comprises the following steps: preparing a single reference solution and a mixed reference solution of chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A and isochlorogenic acid C, and preparing a test solution; after high performance liquid chromatography detection, the relative correction factors of other components in the honeysuckle are calculated by taking chlorogenic acid as an internal reference substance through chromatographic peak positioning, and the contents of the components in the honeysuckle are obtained by utilizing the relative correction components, so that the synchronous determination of the multiple components in the honeysuckle is realized. The method has the advantages of cost saving, simplicity in operation, high detection accuracy and good stability, has great significance in quality control and clinical curative effect guarantee of the honeysuckle medicinal material, and provides a basis for establishing a quality evaluation standard of the honeysuckle medicinal material.

The present invention proposes a method for simultaneously determining the contents of six active ingredients of the Zhuang medicine Sophora flavescens by HPLC, which belongs to the technical field of traditional Chinese medicine analysis. The method comprises the following steps: S1. Preparation of standard stock solution; S2. Preparation of standard mixed solution; S3. Preparation of test solution; S4. Chromatographic conditions; S5. Determination. The present invention compares and analyzes the content determination results of 10 batches of samples and finds that there are differences in the average contents of the six components of neochlorogenic acid, chlorogenic acid, caffeic acid, cryptochlorogenic acid, isochlorogenic acid A, and isochlorogenic acid C in different batches of Sophora flavescens medicinal materials. The present invention establishes a method for simultaneously determining the contents of the six components of neochlorogenic acid, chlorogenic acid, caffeic acid, cryptochlorogenic acid, isochlorogenic acid A, and isochlorogenic acid C in the Zhuang medicine Sophora flavescens, which will provide a theoretical basis for the quality control and standardized planting of Sophora flavescens.

The invention discloses a method for simultaneously preparing high-content isochlorogenic acid A and high-content isochlorogenic acid C. The method comprises the following steps: dissolving a stevia rebaudiana polyphenol product in purified water, and adjusting the pH value to obtain a treating fluid 1; adsorbing the treating fluid 1 through macroporous adsorption resin, jacking the treating fluid 1 through purified water, and mixing effluent liquid during adsorption and purified water jacking to obtain effluent liquid 1; concentrating the effluent 1 by using a reverse osmosis membrane, adjusting the pH value to obtain a treating fluid 2, and extracting the treating fluid 2 by using an ethyl acetate saturated aqueous solution to obtain a raffinate phase I and an extract phase I; adjusting the pH value of the raffinate phase I, and extracting with an ethyl acetate-n-butyl alcohol saturated aqueous solution to obtain a raffinate phase II and an extract phase II; after drying the extract phase I, sequentially crystallizing and recrystallizing to obtain high-purity isochlorogenic acid C; and drying the extract phase II, and sequentially crystallizing, pulping and purifying to obtain the high-purity isochlorogenic acid A. The method comprises the following steps: removing impurities by adopting macroporous adsorption resin, extracting by adopting a solvent step by step, carrying out crude separation on the isochlorogenic acid A and the isochlorogenic acid C, and carrying out subsequent crystallization and refining to obtain the high-content isochlorogenic acid A and the high-content isochlorogenic acid C.

The present invention belongs to the technical field of analytical chemistry, and specifically relates to a quality detection method for a traditional Chinese medicine composition for treating gynecological blood diseases. The quality detection method includes a thin layer chromatography qualitative detection method and a content detection method. The thin layer chromatography qualitative detection method uses isochlorogenic acid A and isochlorogenic acid B as reference substances, and is used in conjunction with Yunnan Guiyina Balsamifera reference medicinal materials to identify the sample. The method is used to analyze the sample, and the obtained thin layer chromatography separation effect is good, the spots are round and clear, and the development rate is fast. The result is easy to judge, and the sample can be quickly and accurately identified. The content detection method of the present invention has a relatively appropriate peak time and good separation. After methodological investigation, it was found that the specificity, stability, repeatability, intermediate precision, accuracy, etc. of the two methods all meet the requirements. The quality detection method provided by the present invention can be used to comprehensively control the quality of the traditional Chinese medicine composition, and further ensure the stability and controllability of the efficacy of the traditional Chinese medicine composition.

This invention discloses a method for extracting chlorogenic acids from stevia waste and its applications, including: extracting chlorogenic acids from stevia powder using an alkaline extraction method; and determining whether the chlorogenic acids contain chlorogenic acid, isochlorogenic acid A, and cryptochlorogenic acid. Using stevia waste as raw material, this invention involves solvent extraction, centrifugal filtration, reflux extraction, and chromatographic separation to obtain a fat-soluble extract. This extract is then dissolved and filtered with ethanol, evaporated and concentrated, and freeze-dried to obtain the chlorogenic acid product. This invention extracts and develops chlorogenic acids from stevia waste, solving the problems of environmental pollution and resource waste. The extracted chlorogenic acids contain chlorogenic acid, cryptochlorogenic acid, and isochlorogenic acid A, and have low sugar content. They possess antioxidant, anti-inflammatory, and blood pressure-lowering functions, and can be widely used in food, pharmaceuticals, and health products, demonstrating high application value and realizing the comprehensive utilization of stevia.

The invention belongs to the technical field of traditional Chinese medicine component detection and analysis, and particularly relates to a method for detecting various active components in lonicerae flos by quantitative analysis of multi-components by single marker. According to the QAMS detection method for the various active ingredients in the lonicera confusa, based on a simple, convenient and rapid high performance liquid chromatography technology, QAMS of the active ingredients such as flavonoids, flavonoid glycosides, iridoid glycosides and organic acids in the lonicera confusa can be achieved, and qualitative and quantitative detection of the various active ingredients is included. The detection target objects comprise 14 active components, namely neochlorogenic acid, cumaric acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, swertiamarin, secologanin, rutin, quercitrin, galuteolin, ferulic acid, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C. The invention further provides a method for detecting the content of the isochlorogenic acid. Tests prove that the method disclosed by the invention meets methodological requirements, has the characteristics of simplicity and convenience in operation, rapidness in detection, high sensitivity, accuracy in determination and strong applicability, is beneficial to accurately evaluating the quality of the lonicerae flos and provides a reliable basis for safe application of the lonicerae flos.

The present application relates to the technical field of pharmaceutical compositions, and provides a composition for improving immune function, a preparation and a preparation method and application thereof, comprising: 25-40% of chlorogenic acid, 5-12% of luteolin, 10-20% of isochlorogenic acid A, 3-8% of caffeic acid, 0.5-1% of a targeting ligand, 2-5% of a microecological component, and 5-8% of a nano-carrier, and application of the composition in preparation of medicines, health products and functional foods for improving immune function, and a preparation method of the above-mentioned medicine preparation, comprising the following steps: preparing a core particle; coating the core particle with a sustained-release enteric coating to obtain a microcapsule particle; filling the microcapsule particle into a capsule shell to obtain an enteric capsule; coating the enteric capsule with a stomach-dissolving coating liquid to obtain a capsule preparation; and achieving the effect of improving the whole gastrointestinal immune function by rapidly targeting anti-inflammation in the stomach and continuously targeting regulation in the intestinal tract, and greatly improving the action efficiency and immune regulation effect of the preparation.

The present invention discloses a method for simultaneously detecting six components of a Qingkailing preparation, the method comprising the following steps: preparing a test solution and a reference solution; adding the test solution and the reference solution to a thin layer plate respectively; using ethyl acetate, acetone, formic acid, and water in a volume ratio of (22-28):(12-18):(1-5):(2-7) as a developing agent to fully react with the thin layer plate obtained in the previous step; and coloring the thin layer plate after the reaction under 250-260 nm ultraviolet light. The method can simultaneously qualitatively detect six effective components of traditional Chinese medicine, namely, geniposide, chlorogenic acid, baicalin, luteolin, isochlorogenic acid A, and isochlorogenic acid C. The operation is simple and fast, the color development results are clear and easy to interpret, and the method has good specificity and reproducibility, thereby achieving quality control of compound traditional Chinese medicine preparations, standardizing drug production, improving drug safety, and improving production process stability and controllability. The method has broad application prospects in the identification and quality control of components of compound traditional Chinese medicine preparations.

The present application relates to a kind of methods for screening anti-inflammatory, antioxidant active compounds from natural Chinese herbal medicine wormwood based on spectrum-effect relationship, the method establishes the method for the common characteristic peak of the fingerprint chromatogram of wormwood extract ultra-high performance liquid chromatography.By grey correlation analysis, Pearson bivariate correlation analysis method and partial least square regression analysis etc. chemometrics method establishes the spectrum-effect relationship of wormwood anti-inflammatory, antioxidant activity, to screen the anti-inflammatory, antioxidant active compounds in wormwood.The analysis result shows that compound chlorogenic acid, isochlorogenic acid A, isochlorogenic acid C is the main anti-inflammatory, antioxidant active compound in wormwood extract.The present application provides scientific and effective method for the research of the medicinal material basis of wormwood and quality control, and provides reference for the development and utilization of wormwood.

The application relates to the field of biological medicine, and discloses self-assembled nanoparticles and a preparation method and application thereof. The self-assembled nanoparticles are formed by self-assembly of isoeuropetin and isochlorogenic acid C through non-covalent bond interaction to form supramolecules, and the particle size of the self-assembled nanoparticles is 50-200 nm, and the Zeta potential is-14.8 mV to-35.2 mV. Based on the self-assembly characteristics of active small molecules of traditional Chinese medicine, it is found and confirmed that EUP and IAC can be self-assembled to form stable nano drugs (EUP-IACs) through various technical means. The nano drugs significantly enhance the inhibiting effect on MRSA USA 300 through the synergistic effect of EUP and IAC, and the application provides a new idea and strategy for optimizing the preparation of traditional Chinese medicine carrier-free self-assembled nano and expanding the application of the traditional Chinese medicine in the antibacterial field.

The invention provides a method for detecting HPLC (high performance liquid chromatography) characteristic chromatograms of senecio scandens medicinal materials, decoction pieces, standard decoction and formula granules, which comprises the following steps: pre-treating raw materials of a test sample to obtain liquid to be detected; the pretreatment mode comprises solvent dissolution and extraction; measuring the liquid to be measured by adopting HPLC (High Performance Liquid Chromatography) to obtain HPLC characteristic chromatograms of the senecio scandens medicinal material, decoction pieces, standard decoction and formula granules; the preparation method comprises the following steps: preparing a reference substance solution, namely dissolving neochlorogenic acid, protocatechuic aldehyde, chlorogenic acid, caffeic acid, cryptochlorogenic acid, p-coumaric acid, chlorogenic acid methyl ester, rutin, hyperoside, isoquercitrin, chlorogenic acid ethyl ester, isorhamnetin-3-O-galactoside, quercitrin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, quercetin and kaempferol by adopting a solvent; obtaining a reference substance solution of the reference substance; the HPLC conditions are as follows: a chromatographic column is a phenyl column; a mobile phase A is an acetonitrile solution, a mobile phase B is a 0.3% phosphoric acid aqueous solution, and gradient elution is carried out; according to the method, a high performance liquid chromatography method is adopted, and an acetonitrile-0. 3% phosphoric acid solution is selected as a mobile phase for gradient elution; the method comprises the following steps: taking neochlorogenic acid, protocatechuic aldehyde, chlorogenic acid, caffeic acid, cryptochlorogenic acid, p-coumaric acid, chlorogenic acid methyl ester, rutin, hyperoside, isoquercitrin, chlorogenic acid ethyl ester, isorhamnetin-3-O-galactoside, quercitrin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, quercetin and kaempferol as reference substances; the HPLC characteristic chromatogram method of the senecio scandens medicinal material, the decoction pieces, the standard decoction and the formula granules is established, and more scientific technical means are provided for controlling the medicinal quality of the senecio scandens medicinal material, the decoction pieces, the standard decoction and the formula granules.

The present disclosure discloses a method for constructing a chicory poria complex fingerprint, including (1) preparing a sample solution of a chicory poria complex, (2) preparing a mixed control solution using chlorogenic acid, 6'-O-β-D-glucosylgentiopicroside, swerthiamarin, gentiopicroside, swelloside, 3-O-caffeoylshikimic acid, neoastilbin, astilbin, rutin, isoastilbin, isochlorogenic acid C, quercitrin, kaempferol-3-O-rutinoside, and narcissine, and (3) injecting the sample solution and the mixed control solution into a high-performance liquid chromatograph, respectively, and performing a similarity analysis on the resulting chromatograms to obtain a chicory poria complex fingerprint. The present disclosure also provides a chicory poria complex fingerprint constructed by the construction method and its application to identifying and / or evaluating the quality of chicory poria complexes. The disclosed chicory poria compound fingerprint can comprehensively, objectively, and accurately detect and evaluate the quality of chicory poria compound, thereby ensuring safe and effective medication for patients.

The application belongs to the field of traditional Chinese medicine quality analysis and control, and discloses a determination method of ingredients content of scabiosa comosa, which takes isochlorogenic acid A as an internal reference, calculates the relative correction factors of chlorogenic acid, caffeic acid, jinyingzhi, 11(13)-dehydroxy axillary ivy chrysanthemum, isochlorogenic acid C, 2,3,4,5-tetra caffeoyl-D-glucaric acid, 2-deoxy-4-tablet-Tianrenjuling, scabioside lactone ketone and terlene lactone, quickly and accurately detects the content of 10 kinds of ingredients of scabiosa comosa, and solves the problems of many required control products, difficulty in obtaining and high price in the determination of scabiosa comosa content, and high detection cost.

The invention provides a method for extracting isochlorogenic acid from plant processing by-products, and particularly belongs to the technical field of plant by-product utilization. The method comprises the following steps: dissolving the plant processing by-product by adopting a first short-chain alcohol solvent to obtain a first mixed solution; mixing the first mixed solution with a non-polar organic extraction solvent, and extracting to obtain an alcohol-water phase; precipitating the alcohol-water phase by adopting a precipitation reagent to obtain an isochlorogenic acid precipitate; dissolving the isochlorogenic acid precipitate in a second short-chain alcohol solvent, mixing with acid, carrying out double decomposition reaction, and carrying out solid-liquid separation to obtain a first filtrate; adjusting the pH value to 2-4, removing short-chain alcohol in the first filtrate, and carrying out solid-liquid separation to obtain an isochlorogenic acid precipitate crude product; and dissolving the isochlorogenic acid precipitate crude product in a third short-chain alcohol solvent, and recrystallizing to obtain the isochlorogenic acid. The method provided by the invention can realize comprehensive utilization of plant processing byproducts, and is simple in process, easy to operate and suitable for large-scale industrial production.

The invention discloses an UPLC (Ultra Performance Liquid Chromatography) identification method for phenolic acid medicinal materials and formula granules, which is characterized in that the number of characteristic peaks of the phenolic acid medicinal materials and the formula granules is determined by a high performance liquid chromatography and is identified by a reference substance. The method can effectively identify medicinal materials and formula granules containing phenolic acid components. The preparation method comprises the following steps: preparing chlorogenic acid; chlorogenic acid; cryptochlorogenic acid; isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid A; isochlorogenic acid C; rutin; linarin; by analyzing the characteristic chromatograms of the traditional Chinese medicinal materials and formula granules and identifying the traditional Chinese medicinal materials and formula granules containing the components, a traditional Chinese medicinal preparation standard containing phenolic acid components is established, so that the detection and analysis of samples are facilitated, and the detection time and cost are saved.

The invention belongs to the technical field of analysis and detection, and provides a method for identifying astringent phenolic acids in coffee and application of the method, phenolic acid compounds in coffee are qualitatively and quantitatively determined through an LC-MS / MS technology, and astringent compounds with the greatest contribution in a coffee sample are determined according to a taste recognition threshold concentration so as to identify key astringent phenolic acids. On the basis, the interaction mechanism of key astringent phenolic acids with different structures and concentrations and salivary protein models is analyzed through binding constants, binding sites, interaction force and thermodynamic parameters. The astringent taste intensity of phenolic acid is evaluated by turbidity, particle size and Zeta potential, the structure-effect and dose-effect relationship between phenolic acid and astringent taste intensity is established, and an application basis is provided for regulation and control of astringent taste of coffee. Results show that in astringent phenolic acid, the astringent perception threshold value of isochlorogenic acid B is lowest, the Dot value is highest, the astringent ability of isochlorogenic acid B is highest, and the content of isochlorogenic acid B needs to be reduced or the reaction of isochlorogenic acid B and sialoprotein needs to be inhibited when astringent of coffee is regulated.

The invention belongs to the technical field of component detection, and particularly relates to a modeling method and a detection method for near-infrared quantitative analysis of stevia rebaudiana components. According to the method, stevioside, rebaudioside A, chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, isochlorogenic acid A, isochlorogenic acid B or isochlorogenic acid C are extracted through a heating reflux synchronous extraction technology, and compared with ultrasonic extraction, the extraction efficiency of the heating reflux synchronous extraction technology for extracting stevia rebaudiana powder samples with the same mass is higher; the real content of the components in the stevia rebaudiana can be reflected. According to the method, a stable and accurate analysis model is established on the basis of the near infrared spectrum technology, then quantitative analysis on stevioside, rebaudioside A, chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, isochlorogenic acid A, isochlorogenic acid B or isochlorogenic acid C in stevia rebaudiana is achieved, the detection speed is high, and the analysis precision is high. Compared with HPLC (High Performance Liquid Chromatography) detection, the detection method provided by the invention does not need to use a large amount of organic solvents and avoids environmental pollution.

The invention relates to the technical field of sweet potato peel polyphenols, and discloses an application of sweet potato peel polyphenols in improving the structure and functional characteristics of bovine cheese protein. Polyphenol is combined with cheese eggs, and the polyphenol can be selected from one of isoA chlorogenic acid, isoB chlorogenic acid, isoC chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid and chlorogenic acid; after the sweet potato peel polyphenol is combined with the casein, the secondary structure of the casein is obviously changed, the content of alpha-helix is reduced, and the content of beta-fold, beta-corner and random crimp is increased; the microenvironment of tryptophan residues in the tertiary structure is changed, the thermal stability of casein is remarkably enhanced by adding polyphenol, the thermal transformation temperature of the bovine cheese protein is increased, the solubility is increased to about 90% under specific conditions, the emulsifying activity is increased by about 30%, and the oxidation resistance is greatly improved.

The present invention provides a leek extract, which is prepared from fresh leek using enzyme-assisted extraction technology, macroporous adsorption resin purification, and drying. The extract contains chlorogenic acid, cryptochlorogenic acid, rutin, isochlorogenic acid A, isochlorogenic acid B, and isochlorogenic acid C in an amount of not less than 30% w / w, and a mass fraction of total phenolic acids of not less than 55% w / w. The present invention also provides a preparation method and use of the leek extract. The leek extract of the present invention has a high content of polyphenols and good antioxidant activity, providing a theoretical basis for the development and utilization of leek.

This invention discloses a method for constructing a fingerprint spectrum of a throat-clearing hard candy and a method for detecting its component content, belonging to the field of component detection technology. In this invention, the throat-clearing hard candy sample solution is analyzed by high-performance liquid chromatography (HPLC) to obtain a fingerprint spectrum of the candy; and the content information of the components is obtained through the external standard method, realizing qualitative and quantitative analysis of the components of the throat-clearing hard candy. Specifically, it involves the simultaneous qualitative and quantitative analysis of the following 10 components: gallic acid, neochlorogenic acid, chlorogenic acid, isorhamnetin-3-O-glucose-7-O-rhamnoside, rutin, luteolin, isochlorogenic acid B, isorhamnetin-3-O-glucose, glycyrrhizic acid, and kaempferol, providing a reliable and quantifiable standard for the quality assessment of throat-clearing hard candy. The chromatogram obtained by the method of this invention has the characteristics of stable baseline, high peak resolution, strong specificity, and good peak symmetry.

The invention discloses a method for extracting isochlorogenic acid C from folium artemisiae argyi. The method comprises the following steps: S1, crushing dried folium artemisiae argyi to 20-80 meshes to obtain folium artemisiae argyi powder; s2, carrying out synergistic wall breaking with a deep eutectic solvent-enzyme composite wall breaking solution system according to a solid-liquid ratio of 1: 10-1: 30 g / mL to obtain a wall-broken mixture; s3, adding a magnetic molecularly imprinted polymer Fe3O4 (at) IsoC-MIP to selectively adsorb the isochlorogenic acid C, and separating by a permanent magnet to obtain magnetic particles loaded with the isochlorogenic acid C; and S4, desorbing with an eluent, concentrating under reduced pressure, and freeze-drying to obtain a high-purity product. According to the extraction method, the low-temperature wall-breaking energy consumption is reduced, the solvent dosage is small, the isochlorogenic acid C with HPLC larger than or equal to 95% and the recovery rate larger than or equal to 92% can be obtained through rapid magnetic separation, magnetic particles can be cycled for 20 times, no toxic residues exist, and green, efficient, low-cost and large-scale extraction is achieved.

The application discloses a kind of construction methods of Jujing compound fingerprint, comprising: (1) Jujing compound is made into test solution;(2) using chlorogenic acid, 6'-O-β-D-glucosyl gentiopicroside, swertiamarin, gentiopicroside, swertisin, 3-O-coffeicoyl shikimic acid, astilbin, astilbinin, rutin, isofraxin, isochlorogenic acid C, quercitrin, kaempferol-3-O-rutinoside and zephyranthine preparation mixed reference solution;(3) respectively test solution and mixed reference solution are injected into high performance liquid chromatograph, the similarity analysis is carried out to the obtained chromatogram, and Jujing compound fingerprint is obtained.The application also provides Jujing compound fingerprint obtained by the construction method and its application in identifying and / or evaluating the quality of Jujing compound.The Jujing compound fingerprint of the application can comprehensively, objectively and accurately detect and evaluate the quality of Jujing compound, so as to ensure the safety and effectiveness of patient medication.

The invention discloses a method for improving curcumin embedding capacity of myofibrillar protein, and belongs to the technical field of food processing. The method for improving curcumin embedding capacity of myofibrillar protein comprises the following steps: dissolving myofibrillar protein powder in a low-salt solution, and carrying out ultrasonication to obtain a protein solution; adding an enzyme into the protein solution, then reacting, and deactivating the enzyme to obtain a mixed solution; adjusting the pH value of the mixed solution to 11-13, adding 4, 5-dicaffeoylquinic acid into the mixed solution, and then reacting in a dark place to obtain the isochlorogenic acid C-ultrasonic-assisted enzyme treated myofibrillar protein compound. According to the method, ultrasonic-enzyme and 4, 5-DCQA treatment is combined, so that the structure of the myofibrillar protein is effectively improved, and the curcumin embedding capacity of the myofibrillar protein is remarkably improved.