Content measurement method for Chinese patent medicine prepared from sweet wormwood, honeysuckle and gardenia jasminoides fruit

A determination method and technology of honeysuckle, which can be used in pharmaceutical formulations, antipyretics, drug combinations, etc., can solve problems such as insufficient detection of component content.

Active Publication Date: 2011-11-09
JIANGSU KANION PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the prior art, in the Chinese patent whose publication number is CN 1517124A and application number is 200410000134.X, a kind of geniposide in this Chinese patent medicine (named as "Reduning injection" in the present invention) is disclosed. and chlorogenic acid for content determination, but this method is not comprehensive enough for the detection of the content of main components in Reduning injection. The technical solution provided by the invention is based on the research on the basic components of Reduning injection and the existing content determination method. On the basis of this, in accordance with the "Basic Technical Requirements for Traditional Chinese Medicine Injections" and the relevant provisions of the re-evaluation of Chinese medicine injections, its HPLC fingerprint and content determination method were further optimized, and the HPLC fingerprint and the defined 9 Combining the content determination of each component, a more comprehensive quality control method is established to control the internal quality of Reduning Injection more effectively and quickly, and improve the quality control level.

Method used

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  • Content measurement method for Chinese patent medicine prepared from sweet wormwood, honeysuckle and gardenia jasminoides fruit
  • Content measurement method for Chinese patent medicine prepared from sweet wormwood, honeysuckle and gardenia jasminoides fruit
  • Content measurement method for Chinese patent medicine prepared from sweet wormwood, honeysuckle and gardenia jasminoides fruit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0137] (1) The chromatographic conditions are: Phenomenex C 18 Chromatographic column (4.6 × 250mm, 5 μm); mobile phase A: methanol; mobile phase B: 0.1% phosphoric acid aqueous solution, the system gradient elution procedure is as follows:

[0138]

[0139] (2) Measurement wavelength:

[0140] ① Chlorogenic acid, cryptochlorogenic acid, caffeic acid, geniposide, isochlorogenic acid B, isochlorogenic acid A, and isochlorogenic acid C are determined at a wavelength of 324nm.

[0141] ② The determination wavelength of geniposide and secoxyloganin content is 237nm;

[0142] (3) Preparation of reference solution: accurately weigh neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, geniposide, caffeic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, An appropriate amount of secoxyloganin reference substance, accurately weighed, dissolved in 50% methanol and diluted to make each 1ml contains 0.2831mg of neochlorogenic acid, 0.7528mg of chlor...

Embodiment 2

[0152] (1) The chromatographic condition is: C 18 Chromatographic column; mobile phase A: methanol; mobile phase B: 0.1% phosphoric acid aqueous solution, the system gradient elution procedure is as follows:

[0153]

[0154] (2) Measurement wavelength:

[0155] ① Chlorogenic acid, cryptochlorogenic acid, caffeic acid, geniposide, isochlorogenic acid B, isochlorogenic acid A, and isochlorogenic acid C are measured at a wavelength of 320nm.

[0156] ② The determination wavelength of geniposide and secoxyloganin content is 230nm;

[0157] (3) Preparation of reference solution: accurately weigh neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, geniposide, caffeic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, An appropriate amount of secoxyloganin reference substance, accurately weighed, dissolved in 50% methanol and diluted to make each 1ml contains 0.2752mg of neochlorogenic acid, 0.8264mg of chlorogenic acid, 0.4281mg of cryptochlo...

Embodiment 3

[0167] (1) The chromatographic condition is: C 18 Chromatographic column; mobile phase A: methanol; mobile phase B: 0.1% phosphoric acid aqueous solution, the system gradient elution procedure is as follows:

[0168]

[0169] (2) Measurement wavelength:

[0170] ① Chlorogenic acid, cryptochlorogenic acid, caffeic acid, geniposide, isochlorogenic acid B, isochlorogenic acid A, and isochlorogenic acid C are measured at a wavelength of 330nm.

[0171] ② The wavelength for measuring the content of geniposide and secoxyloganin is 240nm;

[0172](3) Preparation of reference solution: accurately weigh neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, geniposide, caffeic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, An appropriate amount of secoxyloganin reference substance, accurately weighed, dissolved in 50% methanol and diluted to make each 1ml contains 0.2564mg of neochlorogenic acid, 0.8254mg of chlorogenic acid, 0.4465mg of cryptoc...

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Abstract

The invention belongs to the field of Chinese medicine analysis and particularly relates to a content measurement method for Chinese patent medicine prepared from sweet wormwood, honeysuckle and gardenia jasminoides fruit. The method measures the content of nine ingredients including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, geniposide, secoxyloganin, isochlorogenic acid B, isochlorogenic acid A and isochlorogenic acid C in the Chinese patent medicine by reversed-phase high performance liquid chromatography, wherein the chromatographic conditions include: a chromatographic column is C18 column; methanol or acetonitrile is used as a flowing phase A; 0.05-to-1.0 percent phosphoric acid or 0.05-to-1.0 percent acetic acid is used a flowing phase B; the totalpercentage of flowing phases A and B is 100 percent; and gradient elute is performed. The content measurement method has high repeatability; and the method completely reflects the main ingredients and the change of the main ingredient content of the medicine from a qualitative prospect respectively and thus improves the level of the control over the quality of the medicine.

Description

Technical field: [0001] The invention belongs to the field of traditional Chinese medicine analysis, and relates to a method for determining the content of a Chinese patent medicine, in particular to a method for measuring the content of a Chinese patent medicine prepared from artemisia annua, honeysuckle and gardenia. Background technique: [0002] The original medicinal materials of the Chinese patent medicine involved in the present invention are composed of: 6-25 parts by weight of Artemisia annua, 3-15 parts by weight of Honeysuckle, and 3-12 parts by weight of Gardenia. The functions and indications of this Chinese patent medicine are clearing away heat, dispelling wind, and detoxifying. It is used for symptoms such as high fever, slight aversion to wind-cold, head and body pain, cough, and yellow phlegm caused by upper respiratory tract infection (exogenous wind-heat syndrome). The Chinese patent medicine has been approved for marketing by the State Food and Drug Admi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/744A61P11/00A61P29/00G01N30/02G01N30/89
Inventor 萧伟王振中毕宇安宋爱华刘涛章晨峰付小环陈凤龙
Owner JIANGSU KANION PHARMA CO LTD
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