128 results about "Hplc fingerprint" patented technology

The invention relates to the field of establishment of fingerprints of traditional Chinese medicinal materials, in particular to an establishment method and application of a salvia miltiorrhiza polysaccharide HPLC (High Performance Liquid Chromatography) fingerprint. The establishment method comprises the following steps: preparation of a test solution: taking a salvia miltiorrhiza polysaccharide hydrolysis solution, carrying out derivatization in a reaction bottle, and passing through a 0.1-0.3 [mu] m microporous filter membrane; preparing a reference substance solution, namely weighing mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose reference substances, and preparing into a mixed reference substance solution; performing HPLC (high performance liquid chromatography) determination; and establishing the salvia miltiorrhiza polysaccharide HPLC fingerprint spectrum. According to the method, the difference of monosaccharide composition and content in salvia miltiorrhiza medicinal materials from different producing areas and the spectrum-effect relationship between antioxidant activity and anticoagulant activity are clarified, and a certain reference basis is provided for quality control and evaluation of the salvia miltiorrhiza medicinal materials from the aspect of polysaccharide.

The invention provides a construction method of a fingerprint spectrum of Xinbao pills. The construction method comprises the following steps: 1) preparing a test solution and a mixed reference solution; and 2) constructing the fingerprint spectrum of the Xinbao pill: respectively taking the test solution and the mixed reference substance solution, and performing high performance liquid chromatography detection to obtain the fingerprint spectrum of the Xinbao pill. The invention belongs to the technical field of traditional Chinese medicine preparation detection, and provides a construction method of a fingerprint spectrum of a Xinbao pill, the method is simple and easy to implement, has the advantages of good precision, stability and repeatability and the like, can realize detection of multiple components of the Xinbao pill, and well reflects the overall quality condition of the Xinbao pill; meanwhile, the established HPLC fingerprint spectrum of the Xinbao pill is rich in detection components, totally 21 common characteristic peaks are determined and cover anti-heart-failure active components in ginseng, pseudo-ginseng, venenum bufonis and flos daturae medicinal materials in the Xinbao pill, reference can be provided for comprehensively controlling the internal quality of the Xinbao pill, and the clinical curative effect of the product is further ensured.

The invention belongs to the technical field of quality control of traditional Chinese medicines, and particularly relates to an establishment method of an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of a kidney-tonifying and liver-nourishing mixture and a multi-index component content determination method. The establishment method of the fingerprint spectrum comprises the following steps: mixing the kidney-tonifying and liver-nourishing mixture and a methanol aqueous solution, carrying out ultrasonic treatment, carrying out HPLC detection on an obtained test solution and a mixed reference substance solution, and introducing an obtained chromatogram into fingerprint spectrum software for treatment, so as to obtain the HPLC fingerprint spectrum of the kidney-tonifying and liver-nourishing mixture. The pretreatment method disclosed by the invention is simple, the characteristic components are completely reserved, and the high performance liquid chromatography is adopted, so that the precision is high, the reproducibility is good, and the stability is good; according to the fingerprint spectrum obtained by the invention, 15 common peaks are determined, the quality of the kidney-tonifying and liver-nourishing mixture can be effectively monitored, and a guarantee is provided for comprehensive and effective control of the quality of the kidney-tonifying and liver-nourishing mixture.

The invention belongs to the technical field of processing and quality control of traditional Chinese medicine decoction pieces, and particularly relates to a processing technology of simmered polygala tenuifolia decoction pieces, an HPLC fingerprint spectrum of the simmered polygala tenuifolia decoction pieces, a construction method of the HPLC fingerprint spectrum and an identification method of the HPLC fingerprint spectrum. The processing technology of braised polygala tenuifolia is standardized, parameterization of the processing technology is achieved, and the adopted electromagnetic braised medicine machine is safe, stable in performance, environmentally friendly, controllable in parameter, good in batch-to-batch quality consistency of decoction pieces and suitable for industrial production. The method solves the problems of fragility of containers, poor safety, high labor intensity, uneven quality and the like in the traditional braising method, is green and environment-friendly, meets the GMP (Good Manufacturing Practice) requirements, and can improve the production efficiency by more than 20%. The invention further provides the HPLC fingerprint spectrum, a construction method of the HPLC fingerprint spectrum and an HPLC fingerprint spectrum identification method. The simmered polygala tenuifolia decoction pieces can be scientifically identified through the fingerprint spectrum.

The invention discloses establishment and application of a white kidney bean HPLC (High Performance Liquid Chromatography) fingerprint spectrum. The HPLC fingerprint spectrum of 114 batches of white kidney bean methanol extracts from different geographical sources is established, 9 common peaks are identified, and the spectrum-effect relationship between the 9 characteristic peaks of the HPLC fingerprint spectrum and the alpha-amylase inhibition rate is established through grey correlation analysis and partial least squares regression analysis. The invention finds that the myricetin component is highly correlated with the activity index of the alpha-amylase inhibitor and is possibly a key component of the hypoglycemic activity of the white kidney beans, and provides methodological reference for evaluating the hypoglycemic activity and quality control of the white kidney beans and also provides reference for development and utilization of the white kidney beans.

The present invention discloses a method for constructing a fingerprint spectrum of Cordyceps sinensis and its application. The method includes preparation of a test solution, preparation of a reference solution, preparation of a reference solution and high performance liquid chromatography detection, and the formulation of a fingerprint spectrum of Cordyceps sinensis sterol components. The liquid chromatography conditions of the present invention can effectively separate and detect the sterol components in Cordyceps sinensis, and the separation effect is good. This is the first HPLC fingerprint spectrum detection and identification method for sterol components in Cordyceps sinensis; the method provides favorable data support for the authenticity identification and / or quality control of Cordyceps sinensis and its products.

The invention discloses a dysphagia pill fingerprint spectrum establishment method and an established fingerprint spectrum, belongs to the technical field of analysis and detection of traditional Chinese medicine preparations, and provides a dysphagia pill fingerprint spectrum establishment method which comprises the following steps: mixing a dysphagia pill test sample with petroleum ether, performing Soxhlet extraction, mixing medicine residues with a methanol aqueous solution, performing heating reflux, performing column chromatography, and performing freeze drying to obtain a dysphagia pill fingerprint spectrum. Taking filtrate to obtain a test solution; mixing ellagic acid, liquiritin, ammonium glycyrrhizinate and sesamin reference substances with methanol to obtain a mixed reference substance solution; sucking the test solution and the reference solution, injecting into a liquid chromatograph, and collecting a chromatogram; introducing the chromatogram of the test sample into a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system, selecting common chromatographic peaks as common peaks, generating a dysphagia pill contrast fingerprint spectrum, and calculating the relative retention time and the relative peak area of each common peak to obtain the HPLC fingerprint spectrum of the dysphagia pill. The quality of the dysphagia pill can be comprehensively evaluated by using the map, and the quality stability, consistency and controllability of the dysphagia pill are ensured.

The invention discloses a traditional Chinese medicine formula for treating anaphylactoid purpura, and relates to the technical field of traditional Chinese medicine prescriptions, and the traditional Chinese medicine formula comprises the following raw materials in parts by weight: 80-120 parts of folium isatidis, 80-120 parts of herba cepbalanoplosis segeti, 80-120 parts of cornu bubali, 80-120 parts of hairyvein agrimony, 80-120 parts of carbonized sanguisorba root, 80-120 parts of rehmannia, 80-120 parts of moutan bark, 80-120 parts of pseudo-ginseng, 80-120 parts of radix paeoniae alba, 80-120 parts of astragalus membranaceus, 80-120 parts of selaginella tamariscina and 80-120 parts of bighead atractylodes rhizome. Through the multi-target synergistic effect of removing heat to cool blood, astringing to stop bleeding and tonifying qi to strengthen superficies, the curative effect of anaphylactoid purpura is remarkably improved, and the traditional Chinese medicine composition is free of hormone dependence risk and especially suitable for long-term use by children and chronic patients; the dissolution rate of active ingredients is optimized by adopting a step-by-step extraction process, and the quality is controllable in combination with an HPLC fingerprint spectrum; the pharmaceutical composition is expanded to immune thrombocytopenia and other indications, and has safety, stability and clinical application flexibility.

The invention belongs to the technical field of quality detection of traditional Chinese medicines, and particularly relates to an HPLC (high performance liquid chromatography) fingerprint spectrum detection method of a Qingyanli tablet, which comprises the step of detecting the Qingyanli tablet by adopting a high performance liquid chromatography, and the chromatographic conditions are as follows: an acetic acid aqueous solution with the mass concentration of 1% is used as a mobile phase A, acetonitrile is used as a mobile phase B, gradient elution is performed, and the HPLC fingerprint spectrum detection method is used for detecting the Qingyanli tablet. According to the method disclosed by the invention, each chromatographic peak is well separated, the characteristic peaks are more, the peak shape is good, the separation degree is better, the detection can be more accurate, the method has better detection stability, consistency and controllability, and the quality of the throat clearing tablets can be more comprehensively and reliably detected.

The invention belongs to the field of preparation analysis, and discloses a method for establishing an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of detoxification and hematogenesis granules. According to the invention, a method of combining the HPLC fingerprint spectrum and the chemical mode of the detoxification and hematogenesis granules is established, the contents of the deacetylasperuloside, the rosmarinic acid, the scutellarin, the luteolin, the apigenin and the kaempferol in the detoxification and hematogenesis granules are detected at the same time, and a comprehensive quality evaluation model of the detoxification and hematogenesis granules with different batch numbers is established, so that the analysis result is comprehensive and objective; the method is simple, convenient, reliable, good in repeatability, accurate, stable and high in sensitivity, the multi-component content determination method provides scientific support for quality supervision of the Zhuang medicine preparation detoxification and hematopoiesis granules, and the method can be used for comprehensive quality evaluation and control of the detoxification and hematopoiesis granules. Therefore, the production stability of product quality and the safety and effectiveness of clinical medication are ensured.

The invention discloses a heart-protecting granule fingerprint spectrum construction and component quantitative detection method based on HPLC (High Performance Liquid Chromatography), and belongs to the technical field of pharmaceutical analysis. The method comprises the following steps: preparing heart-protecting granules into a test solution by adopting a high performance liquid chromatography (HPLC) technology, affiliating and identifying chromatographic peaks in the test solution by taking chromatograms of a single medicinal material decoction piece solution and a standard solution as a contrast, and constructing an HPLC fingerprint spectrum containing 12 common characteristic peaks. And a foundation is laid for the establishment of a comprehensive quality control and quality evaluation method of the heart-protecting granules. The method disclosed by the invention can also be used for quantitatively detecting important components, namely naringin and salvianolic acid B, in the heart-protecting granules, has the advantages of high precision, good reproducibility, good stability, high recovery rate, good durability and accurate determination result, and provides technical support for high-precision quality monitoring and evaluation of the heart-protecting granules.

The invention discloses a compound wintercreeper capsule high performance liquid chromatography fingerprint spectrum analysis method and application thereof, and belongs to the technical field of chemical analysis. A mixed solution of protocatechuic acid, calycosin glucoside, formononin, calycosin and formononetin is used as a standard solution; performing high performance liquid chromatography analysis by taking a methanol extracting solution of the compound wintercreeper capsule as a test solution, and establishing an HPLC fingerprint spectrum of the compound wintercreeper capsule; wherein in the high performance liquid chromatography analysis, an acetonitrile-0. 2% phosphoric acid solution is used as a mobile phase, the elution mode is gradient elution, and the detection wavelength is 254 nm. The method provided by the invention is good in repeatability and high in durability, and can be used for controlling the quality of the compound wintercreeper capsule.

The present invention relates to the technical field of traditional Chinese medicine quality detection, and specifically relates to a method for detecting the quality of processed traditional Chinese medicine based on spectral analysis, including: obtaining a reference value of characteristic wave numbers according to the HPLC fingerprint of a traditional Chinese medicine sample, selecting the processed traditional Chinese medicine sample and placing it at the root node, taking the reference value of the first randomly selected characteristic wave number as the splitting value, otherwise setting the splitting value as a random number between the maximum value and the minimum value of the sample, and establishing a second isolation forest; in the second isolation forest, encoding the traditional Chinese medicine samples in the right subtree and the left subtree of the isolation tree as the first value and the second value respectively, calculating the probability that the target characteristic wave number is qualified according to the final possibility, the difference value of the tree depth between the traditional Chinese medicine sample in the isolation tree and the target characteristic wave number, and the encoding information, and calculating the anomaly score of the traditional Chinese medicine sample according to the probability; determining the quality of the traditional Chinese medicine sample to be tested according to the anomaly score of the reference sample of the traditional Chinese medicine sample to be tested. Using the present invention can greatly improve the accuracy of medicinal material quality detection.

The present invention discloses a multi-component quality control method for Tianfoshen oral liquid, which comprises (1) preparing a test solution: (2) preparing a mixed reference solution; determining the HPLC fingerprint of the Tianfoshen oral liquid and calibrating the effective components. The present invention screens out the best mobile phase composition, detection wavelength, chromatographic column, column temperature and other analytical conditions through a large number of experiments. It is shown through multiple experimental verifications that the detection method provided by the present invention can simultaneously detect multiple active components such as ginsenosides and asparagus saponins with similar polarity, so that the quality of the Tianfoshen oral liquid can be comprehensively, objectively and accurately detected and evaluated, which is of great significance for ensuring clinical efficacy.

The application discloses an evaluation method for distinguishing different quality of momordica grosvenori of different origins. The application adopts high performance liquid chromatography to establish the fingerprint of momordica grosvenori, carries out gradient elution by taking water-acetonitrile as a mobile phase, and detects the wavelength of 203 nm to obtain the HPLC fingerprint of momordica grosvenori; the obtained chromatogram is introduced into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system to generate a control fingerprint, different origins of momordica grosvenori are distinguished through similarity comparison and / or chemical pattern recognition analysis; and chemical markers with VIP>1 such as 5-hydroxymethyl furfural, momordica grosvenori flavonoid, momordica grosvenori saponin V and momordica grosvenori saponin IIIA2 are screened. The application can effectively distinguish two main origins of green skin fruit and red hair fruit, overcomes the defects that traditional trait identification is subjective and it is difficult to distinguish similar appearance samples, and provides an objective, accurate and stable technical means for origin identification, quality consistency evaluation and authenticity identification of momordica grosvenori.

The present invention discloses a method for establishing an HPLC fingerprint of Huang'e Capsules, comprising the following steps: (1) preparing a mixed solution of a test sample solution and a reference sample solution; and (2) establishing a fingerprint and identifying common peaks. The present invention performs qualitative and quantitative analysis on multiple active ingredients of multiple medicinal materials, and can more comprehensively reflect the quality information of Huang'e Capsules, ensuring uniform and stable product quality. According to the changing trend and degree of the relative peak area of ​​the reference substance, the key process control links are determined, and corresponding key point quality control standards are formulated. The method can be used for monitoring the production process of Huang'e Capsules, providing a certain theoretical basis and support for the establishment of a full-process quality control system, and ensuring the safety and effectiveness of clinical medication. The present invention also discloses an HPLC fingerprint of Huang'e Capsules and its application.

The invention discloses a quality evaluation method for heart-fire-clearing agilawood eight-ingredient pills. The quality evaluation method comprises the following steps: (1) establishing a fingerprint spectrum of each QCBP test sample solution and calibrating a common peak; (2) carrying out multi-dimensional quality difference evaluation and analysis on a plurality of QCBP test products; (3) if the quality difference exists, screening out key factors causing relatively large difference among the QCBP test samples from the calibrated common peaks of the QCBP test sample solutions; and (4) determining the content of each component causing the quality difference of the sample. According to the invention, based on HPLC-Q-Exactive-MS (High Performance Liquid Chromatography-Quantitative Exactive-Mass Spectrometry) technical component analysis, HPLC fingerprint spectrum establishment and HPLC-MS differential component quantitative evaluation research, a reference is provided for a quality evaluation system of commercially available QCBP.

The present invention relates to a fingerprint construction method and detection method of an Mahuang Dingchuan Decoction composition. The fingerprint construction method of the present invention comprises the following steps: taking a reference substance and an Mahuang Dingchuan Decoction composition, and preparing a reference substance solution and a test solution respectively; using high performance liquid chromatography to detect the reference substance solution and the test solution, and obtaining corresponding detection patterns and reference patterns; importing the detection patterns into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system for analysis, and obtaining a fingerprint of the Mahuang Dingchuan Decoction composition with identification. The present invention constructs a fingerprint of the Mahuang Dingchuan Decoction composition, which has rich chromatographic peaks and contains effective ingredients belonging to multiple medicinal materials such as ephedra, bitter almond, scutellaria, white mulberry bark, ophiopogon, platycodon, coltsfoot flower, radish seed, ginkgo, etc., which can more comprehensively reflect the overall chemical composition of the Mahuang Dingchuan Decoction composition, provide an effective means for the overall quality control and evaluation of the Mahuang Dingchuan Decoction preparation, can better monitor and evaluate the quality of this product, and has a high application value.

The invention relates to and provides a fingerprint spectrum of musk-chuan NaoLiantong granules and a detection method for quantitative analysis of multiple components by single marker. The fingerprint spectrum detection method has the characteristics of simplicity and convenience in operation and good stability and reproducibility. 13 common peaks exist in an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of 15 batches of musk-chuan naoeutong granules, and 7 components in the fingerprint spectrum, namely geniposidic acid, chlorogenic acid, cowherb seed flavonoid glycoside, ferulic acid, senkyunolide I, salvianolic acid B and tanshinone IIA, are identified. The similarity of the 15 batches of the musk and chuan naoliantong granules is 0.961-1. According to the fingerprint spectrum detection method, 13 representative common characteristic peaks are determined so as to comprehensively reflect the internal quality characteristics of the musk-chuan naoliantong preparation.

The application provides a method for establishing HPLC fingerprint of Changnyle capsule (I), which is characterized in that 16 active components including gastrodin, danshensu, quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiopicroside, 3'-hydroxy puerarin, puerarin, 3'-methoxy puerarin, puerarin apioside, paeoniflorin, daidzin, rosmarinic acid, shikonin, isoquercitrin, ononin, daidzein, salvianolic acid B and calycosin are used as reference substances, HPLC is adopted, methanol-acetonitrile-0.5vol% phosphoric acid aqueous solution is used as a mobile phase, different batches of samples are detected, and finally the fingerprint of Changnyle capsule (I) is obtained. The fingerprint can comprehensively represent the main components in Changnyle capsule (I), thereby being beneficial to the quality control of Changnyle capsule (I).

The present invention provides a method for establishing and detecting a fingerprint of a traditional Chinese medicine composition, and specifically relates to a method for establishing and detecting an HPLC fingerprint of a traditional Chinese medicine composition whose raw materials include spinach seeds and gardenia. The method comprises the following steps: preparing a sample of the traditional Chinese medicine composition, accurately weighing it, adding an extraction solvent, ultrasonically treating it, cooling it, centrifuging it, and then filtering it with suction; taking the filtrate and spinning it to obtain a residue; adding a solvent to dissolve the residue, fixing the volume, shaking it well, filtering it, and taking a subsequent filtrate to obtain a sample solution; preparing a reference solution: taking six reference substances, including genipin gentiobioside, gardeniaside, magnolamine, and spinosin, to prepare a mixed reference solution; and using HPLC to perform analysis and determination to complete the construction of a fingerprint. The method has strong specificity, high precision, good stability, and strong repeatability, and provides richer information for the intrinsic quality control of traditional Chinese medicine compositions whose raw materials include spinach seeds and gardenia.

The present invention relates to the fingerprint spectrum of Dapanlongqi medicinal material and the application of multi-component analysis in its quality control. The HPLC fingerprint spectrum of the Dapanlongqi medicinal material is basically consistent with Figure 1, with 14 characteristic fingerprint chromatographic peaks, wherein peak 1 is arbutin, peak 2 is gallic acid, peak 3 is bergenin, peak 5 is catechin, peak 7 is 4-O-galloyl bergenin, peak 9 is 11-O-galloyl bergenin, and peak 12 is catechin gallate.

The invention relates to the technical field of pharmaceutical analysis, in particular to an HPLC (High Performance Liquid Chromatography) fingerprint spectrum construction method and application of a twelve-ingredient numbness pain liniment. The construction method comprises the following steps: preparing a test solution; preparing a reference substance solution; and HPLC determination. The HPLC fingerprint spectrum construction method of the twelve-ingredient numbness pain liniment is provided for the first time, and the method has the advantages of being high in precision, good in reproducibility, high in stability and good in durability. Meanwhile, the method disclosed by the invention can be used for detecting the contents of three components including polydatin, stilbene glucoside and emodin in the twelve-ingredient numbness pain liniment at one time. Technical support is provided for comprehensively improving the product quality standard of the twelve-ingredient arthralgia pain liniment and optimizing the preparation process, identifying true and false and carrying out follow-up material basic research of the twelve-ingredient arthralgia pain liniment.

The invention relates to an HPLC (High Performance Liquid Chromatography) characteristic chromatogram construction method of Tibetan medicine apium graveolens, and belongs to the technical field of detection and analysis. According to the method, a test solution is obtained through a simple and rapid pretreatment method, a high performance liquid chromatograph and a gradient elution mode are adopted, a characteristic spectrum is established, 14 characteristic peaks exist in total, 6 index components of chlorogenic acid, rutin, daidzein, naringin, luteolin and cumin are identified, the relative retention time RSD% of the characteristic peaks is within 3%, and the relative retention time RSD% of the characteristic peaks is within 3%. The wave crest separation of each component is good. According to the method disclosed by the invention, a fingerprint spectrum establishment mode is adopted, so that the medicinal material quality of the apium graveolens can be more effectively ensured, and the blank in the aspect of apium graveolens characteristic spectrum research in the existing standard is filled up.

The application provides a HPLC-based method for constructing a fingerprint spectrum of Tianyang Qianggu Yin and application thereof, and belongs to the technical field of traditional Chinese medicine detection.The method provided by the application comprises the steps of preparing a test sample solution, preparing a mixed control sample solution, HPLC determination, fingerprint spectrum construction and content determination.The application first establishes a HPLC fingerprint spectrum suitable for water-soluble extracts of Tianyang Qianggu Yin compound, and simultaneously realizes accurate content determination of six components, i.e., neotamanoxin, naringin, alpinumisine A, alpinumisine B, alpinumisine C and icariin.The application has strong specificity, high precision, good repeatability and excellent accuracy.The application provides scientific and controllable technical support for the production quality consistency and efficacy stability of Tianyang Qianggu Yin, and can be used for quality control and batch consistency evaluation of the compound preparation.

The application discloses a kind of kidney Yishen and turbidity releasing mixture HPLC fingerprint quality control method, including the preparation of test solution, HPLC chromatographic conditions and the establishment of fingerprint.The application carries out computer similarity evaluation to the chromatographic peak of different batches of kidney Yishen and turbidity releasing mixture, obtains the common peak of different batches of drugs, and generates HPLC control fingerprint, by comparing with the common peak and similarity in HPLC control fingerprint, the quality of kidney Yishen and turbidity releasing mixture can be comprehensively and quickly evaluated, and the chromatographic peak of each single medicine in prescription is attributed to confirm, can trace the material basis of preparation from raw material source, ensure preparation quality.Further quantitative analysis is carried out to 5 chemical components of kidney Yishen and turbidity releasing mixture, to ensure the stability and uniformity of preparation quality.

The purpose of the present invention is to solve the problem that the traditional Chinese medicine ingredients of the existing Guanxintai pill preparations are complex and the effective ingredients are unclear. Determination method: 1. Grind Guanxintai pills, add methanol solution as the extraction solvent, heat and reflux, drop the methanol solution to the weight before heating and reflux, and shake well to obtain the test solution; 2. Take tannic acid B as the reference substance, add methanol solution to prepare the reference solution; 3. Use acetonitrile as the mobile phase A and a phosphoric acid solution with a volume concentration of 0.1% as the mobile phase B for gradient elution; 4. Take the test solution and the reference solution and inject them into the liquid chromatograph respectively. The present invention optimizes the chromatographic conditions, and the obtained fingerprint spectrum has high separation and good peak shape. The results of determining the fingerprint spectrum of Guanxintai pills are precise and have good repeatability and stability. By comparing the presence or absence of common peaks in the obtained fingerprint spectrum, the quality of Guanxintai pills can be comprehensively evaluated.

The present invention discloses a high performance liquid chromatography fingerprint analysis method for compound Fufangteng capsule and its application, which belongs to the field of chemical analysis technology. A mixed solution of protocatechuic acid, calycosin glucoside, thorn formononetin, calycosin, and formononetin is used as a standard solution, and a methanol extract of compound Fufangteng capsule is used as a test solution for high performance liquid chromatography analysis to establish a compound Fufangteng capsule HPLC fingerprint; wherein, the high performance liquid chromatography analysis uses acetonitrile 0.2% phosphoric acid solution as the mobile phase, the elution mode is gradient elution, and the detection wavelength is 254nm. The method provided by the present invention has good repeatability and high durability, and can be used for compound Fufangteng capsule quality control.

The invention belongs to the technical field of medicine detection, and particularly relates to a quality evaluation method for phlegm and fluid retention pills based on HPLC fingerprint spectrum and multi-index component content determination. The method comprises the following steps: determining the contents of nine active components in the phlegm-drinking pill and constructing an HPLC fingerprint spectrum of the phlegm-drinking pill based on a high performance liquid chromatography-high resolution mass spectrometry technology; wherein the nine active ingredients are sinapine, chlorogenic acid, cryptochlorogenic acid, 3, 6 '-dimustard acyl sucrose, rosmarinic acid, apiose isoliquiritin, isoliquiritin, glycyrrhizic acid and atractylenolide I. The invention further discloses a preparation method of the pharmaceutical composition. According to the method, the fingerprint spectrum and multi-index determination are integrated, a reproducible technical framework is provided for modern quality evaluation of the traditional Chinese medicine compound preparation with lower instrument cost and higher universality, and the industry is promoted to be transformed from'single component control 'to'overall quality management and control'.

The application discloses a method for establishing HPLC fingerprint spectrum of Shengteng Qingbi granules, which comprises preparation of a test sample solution, determination of HPLC chromatographic conditions and production of an HPLC standard fingerprint spectrum. The application also discloses the HPLC standard fingerprint spectrum of Shengteng Qingbi granules obtained by the method, which has 35 common peaks. The quality detection method is simple in operation, high in stability and good in reproducibility, and the obtained spectrum has many characteristic peaks. Through comparison of the common peaks of the standard fingerprint spectrum, the quality of Shengteng Qingbi granules can be comprehensively evaluated and controlled, which is beneficial to accurate evaluation of the internal quality of the preparation and ensures the safety and effectiveness of clinical medication.