474 results about "Hplc fingerprint" patented technology

A novel botanical extract from the plant Tripterygium Wilfordii Hook. F. containing bioactive components triptriolide, tripdiolide, tripchloride and tiptolide for treating inflammatory and autoimmune diseases is prepared and the bioactive components are quantified. A HPLC fingerprinting technology is also developed to produce a characteristic fingerprint for the botanical extract.

The invention discloses a method for screening traditional Chinese medicine effect related ingredients and a model building method. The method comprises the steps as follows: taking the researched traditional Chinese medicines as objects for preparing multiple sets of sample solutions containing chemical components with different concentrations; selecting part of the chemical components for HPLC (High Performance Liquid Chromatography) analysis, and investigating linear relations between the peak areas and the concentrations; selecting the multiple sets of sample solutions in the linear range, and determining the respective HPLC fingerprint spectrums and the medicine effect indexes of the sample solutions; and obtaining relations between the peak areas and the medicine effect indexes of the ingredients by adopting a linear regression analysis method in the linear relation range, and confirming the actions of the medicine effect related ingredients on the medicine effect indexes according to the positive value and the negative value of the standard regression coefficients and/or the t check results of the ingredients so as to screen out the traditional Chinese medicine effect related ingredients. The method is simple, convenient, easy to implement, reliable and good in commonality, can embody the characteristics of comprehensive actions of the multiple ingredients on the medicine effect indexes, is suitable for screening the traditional Chinese medicine effect related ingredients, and provides a technical data support for discovering and screening traditional Chinese medicine active ingredients.

The invention relates to the technical field of compound traditional Chinese preparations, and in particular relates to a quality control method of liuwei wuling tablets. The quality control method is characterized in that 20 different proportion groups are formed by randomly sampling six medicinal materials of the liuwei wuling tablets; a corresponding HPLC fingerprint spectrum is established, and in-vitro anti-hepatic fibrosis activity is measured; and an SPSS software is utilized to analyze, so that a pectrum-effect relationship of in-vitro anti-hepatic fibrosis effect of the liuwei wuling tablets is established. The relatively perfect quality control method is established on the basis of pharmacodynamic substances of the liuwei wuling tablets. Compared with a conventional single-index content measuring method, the quality control method is more comprehensive, scientific and standard, and has the advantages that the content of multiple components is simultaneously measured, so that not only the analysis time is shortened, but also the loss of a solvent is saved.

The invention provides a HPLC fingerprint spectrum measurement method for standard double-harmonizing decoction, belonging to the technical field of traditional Chinese medicine. The method comprises the following steps: (1) preparing a test sample solution; (2) preparing a mixed reference solution; (3) preparing a single medicine negative reference solution; (4) precisely absorbing the test sample solution, the mixed reference solution and the single medicine negative reference solution respectively and injecting into a high performance liquid chromatograph and measuring to obtain the liquid chromatogram of the test sample solution, the mixed reference solution and the single medicine negative reference solution; and (5) performing data matching on the liquid chromatogram of the test sample solution, the mixed reference solution and the single medicine negative reference solution by use of the traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system formulated by the Chinese committee of pharmacopeia to obtain a standard fingerprint spectrum. According to the invention, the quality of standard granules of double-harmonizing decoction is effectively controlled, the curative effect of the drug is guaranteed, and more formal quality control is realized on the classical famous prescription.

The present invention provides a method for authenticating dendrobium officinale by using a HPLC fingerprint. The method comprises the following steps: (1) establishing a HPLC fingerprint of dendrobium officinale: a, precisely weighing powder of a dendrobium officinale medicinal material, adding water or a monohydric alcohol to carry out extraction and concentration, extracting by an organic solvent, carrying out treatments of recovering, concentrating, volume metering, filtering to obtain a tested sample solution; b, adopting a high performance liquid chromatography method to analyze the tested sample solution to obtain the fingerprint of the dendrobium officinale medicinal material; (2) establishing a standard fingerprint of the dendrobium officinale: carrying out analysis and comparison for 20 batches of the dendrobium officinale medicinal materials to obtain the standard fingerprint of the dendrobium officinale and the similarity of the dendrobium officinale medicinal material, wherein the similarity is more than 0.8; (3) adopting the same steps to detect the fingerprint of the dendrobium officinale counterfeit/substitute, carrying out comparison for the fingerprint of the dendrobium officinale counterfeit/substitute and the standard fingerprint of the dendrobium officinale, calculating the similarity, wherein the similarity is less than 0.6. According to the present invention, the standard fingerprint and the similarity can be adopted to effectively monitor the authenticity of the dendrobium officinale and the medicinal material quality.

The present invention provides a Chinese medicinal material polygonum capitatum. Said invention provides its HPLC fingerprint chromatogram map, it contains 13 characteristic peaks. Its chromatographic condition is as flows: chromatographic column: Hypersil ODS chromatographic column (4.6mmX150mm, 5micrometers); column temperature: 25deg.C; mobile phase: acetonitrile (A)-0.4% phosphoric acid (B) solution, binary gradient elution, weight percentage is 0-30%: 100-70%; flowing rate: 0.8ml.min-1; detection wavelength: 310nm; and sample size: 20 microliters. Besides, said invention also provides its extract and its quality control method.

The invention discloses a quality control method of Polygonum multiflorum through the fingerprint thereof. The fingerprint is HPLC (high-performance liquid chromatography) fingerprint obtained through HPLC analysis of Polygonum multiflorum. The fingerprint can be used for completely monitoring the quality of Polygonum multiflorum to ensure the stable and uniform quality of Polygonum multiflorum and related pharmaceutical preparations containing Polygonum multiflorum. The invention further discloses the establishment method and the application of the fingerprint of Polygonum multiflorum.

The invention relates to a control method of the fingerprint spectrum quality of cordyceps sinensis powder raw material in botanical drug for strengthening vital qi and removing blood stasis, comprising the steps that: (1) cordyceps sinensis powder is extracted: 0.100g of cordyceps sinensis powder is taken, purified water is added, the ultrasonic extraction, the filtration and the sample injection are carried out; (2) the gradient elution with mobile phase is carried out: octadecyl silane bonded silica gel is taken as a filler, water and acetonitrile are taken as mobile phase to carry out the gradient elution for 0 to 30min and 0 to 7 percent B; (3) a standard fingerprint spectrum is established: the HPLC standard fingerprint spectrum of the cordyceps sinensis powder is determined, and 3 characteristic peaks are selected; (4) the quality control of the fingerprint spectrum is carried out: the relative retention time of No.2 peak uridine, No.3 peak guanosine and No.4 peak adenosine are 0.44 plus or minus 0.03, 0.68 plus or minus 0.03 and 1.00 respectively; the HPLC fingerprint spectrum of the sample is compared with the contrast fingerprint spectrum. The similarity calculated by the 5 common peaks is not less than 0.9, (5) the preparation of the cordyceps sinensis powder raw material is carried out; the control method has good repetitivity and can fully reflect the basic characteristics of nucleoside ingredients of the cordyceps sinensis powder.

The invention relates to an HPLC fingerprint spectrum of Saussurea involucrate and a method for establishing a DNA fingerprint spectrum of the Saussurea involucrate. The invention is characterized in that the HPLC fingerprint spectrum comprises fingerprint spectrums of two extraction positions with different polarities of ethyl acetate and n-butyl alcohol, and the fingerprint spectrum of the extraction position of the ethyl acetate has 20 common peaks, wherein 7 peaks refer to scopoletin, rutoside, isoquercitrin, xylostein, quercetrin, homoplantaginin and hispidulin respectively; the fingerprint spectrum of the extraction position of the n-butyl alcohol has 11 common peaks, wherein 3 peaks refer to bergapten, scopoletin and rutoside; and the DNA fingerprint spectrum consists of an RAPD molecular fingerprint spectrum and an ISSR molecular fingerprint spectrum. The quality and the reality and falsity of the Saussurea involucrate can be universally and effectively detected in the aspects of chemistry and biology by combination of HPLC fingerprint and DNA fingerprint.

The invention relates to a propolis (as a medicinal material) distinguishing method and a preliminary classification method of propolis production areas. First test sample solution is prepared, then control solution is prepared. The measurement method is as follows: 10mul of the reference solution and 10mul of the test sample solution are respectively and accurately extracted and then injected into a high performance liquid chromatograph; the test is performed according to the high performance liquid chromatograph; with the control solution as the reference, the chromatogram is measured and recorded; then a propolis HPLC finger-print map is established. The invention has the advantages of convenient method, stability, high accuracy, good reproduction and easiness in mastering, and also has the advantages that the trueness of the propolis can be mastered from the whole characteristic appearance of the chromatogram, and the propolis distinguishing method can be used as one of the indexes for controlling the propolis quality and distinguishing the trueness of the propolis. Meanwhile the propolis from different production areas also undergoes preliminary classification.

The invention belongs to the field of analysis of traditional Chinese medicinal materials, and particularly discloses an HPLC (High performance liquid chromatography) method for distinguishing different cultivars of uncaria. All chromatographic peaks are separated effectively when the uncaria traditional Chinese medicinal materials are subjected to HPLC detection by optimizing each experimental parameter; the phenomena of mutual interference of the chromatographic peaks, trailing and the like are reduced; mutual interference of the chromatographic peaks of different ingredients in different cultivars of uncaria in HPLC detection is basically avoided, so as to provide basis for authenticating different cultivars of uncaria and constructing a uncaria HPLC fingerprint spectrum.

The invention discloses establishment of an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of rabdosia lophanthide medicinal materials and a fingerprint spectrum of the rabdosia lophanthide medicinal materials, relates to a quality control method of traditional Chinese medicine medicinal materials, and particularly relates to a method for detecting an extracting solution of the rabdosia lophanthide medicinal materials by establishing the HPLC fingerprint spectrum. The method comprises the following steps: preparing a comparison product solution; selecting an extracting solvent and an extracting method; preparing a sample solution; inspecting chromatographic conditions, namely carrying out gradient elution by taking octadecyl bonded silica gel as filler of a chromatographic column, wherein a gradient elution solution is composed of acetonitrile-phosphoric acid with a flowing phase being 0.1%-0.3%, the column temperature is 25-40 DEG C, the ultraviolet detection wavelength is 230-270nm and the time is 100-120min; determining the HPLC fingerprint spectrums of the different types of rabdosia lophanthide medicinal materials; and marking a characteristic peak and selecting a reference peak. By adopting the method disclosed by the invention, the dispute of using rabdosia lophanthide as a medicine base source is solved. Methods including common peak comparison, clustering analysis, characteristic peak analysis and the like of the fingerprint spectrum are comprehensively utilized and are used for identifying the three types of HPLC fingerprint spectrums, so as to realize the base source identification of the rabdosia lophanthide medicinal materials, divide production area classification and provide evidences on quality control and accurate clinical utilization of the rabdosia lophanthide medicinal materials.

The invention discloses a method for establishing an infantile stagnation-removing and cough-relieving oral solution-HPLC (High Performance Liquid Chromatography) fingerprint spectrum. The method comprises the following steps: preparing a tested product solution; determining an HPLC chromatographic condition; making an HPLC standard fingerprint spectrum. The invention also discloses the standard infantile stagnation-removing and cough-relieving oral solution-HPLC fingerprint spectrum obtained by adopting the method. The standard infantile stagnation-removing and cough-relieving oral solution-HPLC fingerprint spectrum has 19 common peaks, the relative retention times tR of the 19 common peaks are sequentially as follows: 0.066, 0.077, 0.088, 0.124, 0.157, 0.166, 0.192, 0.220, 0.229, 0.275, 0.481, 0.837, 0.872, 0.890, 0.962, 1.000, 1.026, 1.061 and 1.203. The method disclosed by the invention has the advantages of easiness and convenience for operation, high stability and good repeatability; the obtained spectrum has the multiple characteristic peaks, can be used for comprehensively evaluating the quality of an infantile stagnation-removing cough-relieving oral solution through the comparison of the common peaks of the standard fingerprint spectrum, is favorable to stabilizing the quality of products and ensuring the safety and the effectiveness of clinical medications.

The invention discloses a processing method of honey-fried liquorice, which can effectively overcome the weaknesses that the quality is unstable for the frying time is subjectively controlled when the prepared liquorice pieces are made into the honey-fried liquorice pieces, and comprises the following steps that: prepared liquorice pieces are obtained to be arranged inside refined honey with water to be mixed and moistened until the refined honey is adequately absorbed by the liquorice, the liquorice pieces are arranged inside a frying container to be fried for a given time, when the surface reaches a given temperature, the fried liquorice pieces are taken out to be cooled to the room temperature, and after the scraping is sieved, the fried liquorice pieces are sealed. The method is simple, saves time and is easy to operate. The prepared honey-fried liquorice pieces have uniform color and brightness. The HPLC fingerprint of the honey-fried liquorice pieces which are made with the traditional processing method and the processing method of the invention are compared, the peak area of main chromatographic peak is proved to be larger than that of the liquorice piece which is made with the traditional processing method. The benefit of the honey-fried liquorice pieces which are prepared with the processing method is proved by the pharmacodynatics to be better than that of the honey-fried liquorice pieces which are prepared with the traditional processing method.

The invention provides a method for the detection of folia eriobotryae or drugs containing folia eriobotryae raw material. The detection method adopting HPLC (high performance liquid chromatography) fingerprint for detection includes the steps of: (1) preparing a test solution; (2) preparing a reference solution by taking chlorogenic acid, neochlorogenic acid and cryptochlorogenic acid; (3) conducting precise drawing of the reference solution and the test solution into a liquid chromatograph and then performing elution with 0.4% of phosphoric acid-acetonitrile as a mobile phase. The quality detection method of the folia eriobotryae or the drugs containing the folia eriobotryae raw material has the advantages of being capable of performing content determination and feature mapping and saving inspection costs, and being capable of distinguishing between folia eriobotryae (medicinal materials, decoction pieces or formula granules) and honeyed folia eriobotryae (medicine, decoction piecesor formula granules) and conducting quality control of the whole process of folia eriobotryae or honeyed folia eriobotryae production.

The invention discloses an HPLC fingerprint detection method of a cyclomastopathy eliminating pill preparation. The method comprises the following steps: preparing a test substance solution, preparing a reference substance solution, and determining the correlation between all bulk drugs and a finished product; and determining by using a high performance liquid chromatograph, calculating the relative retention time and the relative peak area of the test substance with the retention time and the peak area of a reference peak as 1 to obtain the HPLC fingerprint of the cyclomastopathy eliminating pill preparation. The HPLC fingerprint of athe cyclomastopathy eliminating pill preparation obtained through the method shows the numbers of characteristic peaks of radix bupleuri, Rhizoma Cyperi, Rheum officinale, Ligusticum wallichii, Rhizoma Curcumae, Chinese angelica, white peony root, Fritillaria thunbergii Miq., Vaccaria segetalis and green tangerine peel below 254nm are 4, 4, 17, 7, 5, 7, 11, 4, 6 and 11 respectively. Experiment verification results show that the fingerprint detection method has the advantages of high sensitivity and precision, good stability and repeatability, high similarity, objective, comprehensive and accurate evaluation of the quality of the cyclomastopathy eliminating pill preparation, and importance for guaranteeing of the clinic drug effect.

The invention provides a method for establishing an HPLC fingerprint spectrum of Fructus Lycii and a standard fingerprint spectrum thereof. The method comprises the following steps of: preparing a test solution, preparing a reference solution, selecting chromatographic conditions, and carrying out HPLC determination to obtain the fingerprint spectrum of Fructus Lycii. The standard fingerprint spectrum of Fructus Lycii can be obtained by comparing HPLC fingerprint spectra of 21 batches of Fructus Lycii materials and determining the common fingerprint characteristics. The method has the advantages of simple operation, good reproducibility, a plurality of characteristic peaks, accurate and reliable result, etc. The standard fingerprint spectrum can be used for effectively monitoring the quality of Fructus Lycii.

The invention discloses a method for building quality control chromatography fingerprint maps of traditional Chinese medicine herbal tea and herbal medicine beverage products. The method comprises the steps of: (1) preprocessing a sample and processing the sample by one method selected from following methods: (1) a freeing, drying-extracting method, (2) a thermal evaporating, drying-extracting method, and (3) a solid-phase extraction method; (2) conducting HPLC (high performance liquid chromatography) fingerprint map analysis. According to the method, the quality control chromatography fingerprint maps of the traditional Chinese medicine herbal tea and herbal medicine beverage products built by the method disclosed by the invention can be used for carrying out macroscopic and fine constituent quality control on the product, and the blank that the Chinese herbal medicine beverage such as the traditional Chinese medicine herbal tea and herbal medicine herbal tea only has requirements on raw material and auxiliary material, sensory indexes and health indexes in quality control, but has no macroscopic quality control indexes of related phytochemical constituents at present is filled.

The invention relates to a finger-print spectrum mutual mode construction method and a quality detection method of chrysanthemum cell-disruption decoction pieces. In the method, with isochlorogenic acid A as a contrast peak, mutual mode standard spectrums and determination indexes are established through HPLC analysis to not less than 10 batches of the samples under following chromatographic conditions: column temperature: 35 DEG C; wavelength: 348 nm; mobile phase: an acetonitrile-0.5% phosphoric acid solution; elution gradient: 0-8 min-24 min-50 min-75 min; and acetonitrile change: 14%-18%-18%-25%-45%. The spectrums of the samples to be detected under the same chromatographic condition are compared with the mutual mode standard spectrums to detect the quality of the samples to be detected. The invention firstly discloses the HPLC finger-print spectrum and the quality detection method aiming to the chrysanthemum cell-disruption decoction pieces. The spectrums comprehensively contain the spectrum information of main active components of the chrysanthemum cell-disruption decoction pieces. The method is strong in specificity, is quick and accurate in detection, and can effectively control the total quality of medicines, cell-disruption powders and cell-disruption decoction pieces.

The invention relates to an establishing method for HPLC fingerprints of cordyceps fungus nucleoside components in different product forms, and standard fingerprints. The employed chromatographic column is Agilent Zorbax Extend C18 (250 mm*4.6 mm, 5 mu m), the column temperature is 25 DEG C, the detection wavelength is 260 nm, the mobile phase is a methanol-water system, and a gradient elution mode is employed for obtaining of the HPLC fingerprints of nucleoside components of cordyceps stromata, mycelia and encarpia. Through comparison of the HPLC fingerprints of the stromata, mycelia and encarpia of the six cordyceps fungus, common fingerprint characteristics of the three-form cordycepses are respectively determined, and the standard fingerprints are obtained, and qualitative determination and quantitive determination are performed on common fingerprint peaks. The method of the invention has the advantages of simplicity, stability, high precision and good repeatability, and helps to accurately reflect quality state of cordyceps fungus from the integrated chromatographic characteristics of different-form cordycepses. The common fingerprint characteristics can be taken as one of the indexes for cordyceps quality control and discrimination.

The invention discloses a detection method for qualified pinellia tubers. According to the method, determination is performed through HPLC (high performance liquid chromatography) fingerprints and the method comprises the operation steps as follows: (1) taking a to-be-detected sample, crushing the sample, performing extraction with water or methanol serving as a solvent, and preparing a test solution; (2) injecting the test solution into a high performance liquid chromatograph to be determined. Chromatographic conditions are as follows: the detection wavelength is in a range of 254-265 nm; a chromatographic column takes octadecylsilane as filler; and a mobile phase is eluted by a methanol-water system. The detection method for the qualified pinellia tubers is high in precision, good and accurate in reproducibility, convenient to operate, low in cost and broad in market application prospect.

The invention refers to a constructing method for HPLC fingerprint diagram of Rhodiola tibetica (Hook.f.et Thomas) Fu medicinal materials and the standard fingerprint diagram. At first, takes the Rhodiola tibetica (Hook.f.et Thomas) Fu powder, adds in alcohol, carries on microwave extraction, the lead acetate liquid is added into the extraction liquid and generates deposit, it is centrifugated, the acquired supernate is used as testing liquid; then, carries on high efficiency liquid phase color spectrum analysis: absorbs the testing sample liquid, the color spectrum pole is hypersil ODS (5m, 2504.0mm), the flow phase is 0.05% phosphoric acid buffer solution-methyl alcohol, the invention uses ladder eluting method; the testing wavelength: 360nm; acquires the fingerprint diagram of HPLC, confirms the fingerprint character, acquires the standard fingerprint diagram of the medicine. The HPLC fingerprint character can monitor the quality of Rhodiola tibetica (Hook.f.et Thomas) Fu, ensures it is stable, even, and controllable.