83 results about "Hplc fingerprint" patented technology

The invention discloses establishment and application of a white kidney bean HPLC (High Performance Liquid Chromatography) fingerprint spectrum. The HPLC fingerprint spectrum of 114 batches of white kidney bean methanol extracts from different geographical sources is established, 9 common peaks are identified, and the spectrum-effect relationship between the 9 characteristic peaks of the HPLC fingerprint spectrum and the alpha-amylase inhibition rate is established through grey correlation analysis and partial least squares regression analysis. The invention finds that the myricetin component is highly correlated with the activity index of the alpha-amylase inhibitor and is possibly a key component of the hypoglycemic activity of the white kidney beans, and provides methodological reference for evaluating the hypoglycemic activity and quality control of the white kidney beans and also provides reference for development and utilization of the white kidney beans.

The invention discloses a heart-protecting granule fingerprint spectrum construction and component quantitative detection method based on HPLC (High Performance Liquid Chromatography), and belongs to the technical field of pharmaceutical analysis. The method comprises the following steps: preparing heart-protecting granules into a test solution by adopting a high performance liquid chromatography (HPLC) technology, affiliating and identifying chromatographic peaks in the test solution by taking chromatograms of a single medicinal material decoction piece solution and a standard solution as a contrast, and constructing an HPLC fingerprint spectrum containing 12 common characteristic peaks. And a foundation is laid for the establishment of a comprehensive quality control and quality evaluation method of the heart-protecting granules. The method disclosed by the invention can also be used for quantitatively detecting important components, namely naringin and salvianolic acid B, in the heart-protecting granules, has the advantages of high precision, good reproducibility, good stability, high recovery rate, good durability and accurate determination result, and provides technical support for high-precision quality monitoring and evaluation of the heart-protecting granules.

The application discloses an evaluation method for distinguishing different quality of momordica grosvenori of different origins. The application adopts high performance liquid chromatography to establish the fingerprint of momordica grosvenori, carries out gradient elution by taking water-acetonitrile as a mobile phase, and detects the wavelength of 203 nm to obtain the HPLC fingerprint of momordica grosvenori; the obtained chromatogram is introduced into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system to generate a control fingerprint, different origins of momordica grosvenori are distinguished through similarity comparison and / or chemical pattern recognition analysis; and chemical markers with VIP>1 such as 5-hydroxymethyl furfural, momordica grosvenori flavonoid, momordica grosvenori saponin V and momordica grosvenori saponin IIIA2 are screened. The application can effectively distinguish two main origins of green skin fruit and red hair fruit, overcomes the defects that traditional trait identification is subjective and it is difficult to distinguish similar appearance samples, and provides an objective, accurate and stable technical means for origin identification, quality consistency evaluation and authenticity identification of momordica grosvenori.

The present invention discloses a method for establishing an HPLC fingerprint of Huang'e Capsules, comprising the following steps: (1) preparing a mixed solution of a test sample solution and a reference sample solution; and (2) establishing a fingerprint and identifying common peaks. The present invention performs qualitative and quantitative analysis on multiple active ingredients of multiple medicinal materials, and can more comprehensively reflect the quality information of Huang'e Capsules, ensuring uniform and stable product quality. According to the changing trend and degree of the relative peak area of ​​the reference substance, the key process control links are determined, and corresponding key point quality control standards are formulated. The method can be used for monitoring the production process of Huang'e Capsules, providing a certain theoretical basis and support for the establishment of a full-process quality control system, and ensuring the safety and effectiveness of clinical medication. The present invention also discloses an HPLC fingerprint of Huang'e Capsules and its application.

The present invention relates to a fingerprint construction method and detection method of an Mahuang Dingchuan Decoction composition. The fingerprint construction method of the present invention comprises the following steps: taking a reference substance and an Mahuang Dingchuan Decoction composition, and preparing a reference substance solution and a test solution respectively; using high performance liquid chromatography to detect the reference substance solution and the test solution, and obtaining corresponding detection patterns and reference patterns; importing the detection patterns into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system for analysis, and obtaining a fingerprint of the Mahuang Dingchuan Decoction composition with identification. The present invention constructs a fingerprint of the Mahuang Dingchuan Decoction composition, which has rich chromatographic peaks and contains effective ingredients belonging to multiple medicinal materials such as ephedra, bitter almond, scutellaria, white mulberry bark, ophiopogon, platycodon, coltsfoot flower, radish seed, ginkgo, etc., which can more comprehensively reflect the overall chemical composition of the Mahuang Dingchuan Decoction composition, provide an effective means for the overall quality control and evaluation of the Mahuang Dingchuan Decoction preparation, can better monitor and evaluate the quality of this product, and has a high application value.

The invention relates to and provides a fingerprint spectrum of musk-chuan NaoLiantong granules and a detection method for quantitative analysis of multiple components by single marker. The fingerprint spectrum detection method has the characteristics of simplicity and convenience in operation and good stability and reproducibility. 13 common peaks exist in an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of 15 batches of musk-chuan naoeutong granules, and 7 components in the fingerprint spectrum, namely geniposidic acid, chlorogenic acid, cowherb seed flavonoid glycoside, ferulic acid, senkyunolide I, salvianolic acid B and tanshinone IIA, are identified. The similarity of the 15 batches of the musk and chuan naoliantong granules is 0.961-1. According to the fingerprint spectrum detection method, 13 representative common characteristic peaks are determined so as to comprehensively reflect the internal quality characteristics of the musk-chuan naoliantong preparation.

The application provides a method for establishing HPLC fingerprint of Changnyle capsule (I), which is characterized in that 16 active components including gastrodin, danshensu, quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiopicroside, 3'-hydroxy puerarin, puerarin, 3'-methoxy puerarin, puerarin apioside, paeoniflorin, daidzin, rosmarinic acid, shikonin, isoquercitrin, ononin, daidzein, salvianolic acid B and calycosin are used as reference substances, HPLC is adopted, methanol-acetonitrile-0.5vol% phosphoric acid aqueous solution is used as a mobile phase, different batches of samples are detected, and finally the fingerprint of Changnyle capsule (I) is obtained. The fingerprint can comprehensively represent the main components in Changnyle capsule (I), thereby being beneficial to the quality control of Changnyle capsule (I).

The present invention provides a method for establishing and detecting a fingerprint of a traditional Chinese medicine composition, and specifically relates to a method for establishing and detecting an HPLC fingerprint of a traditional Chinese medicine composition whose raw materials include spinach seeds and gardenia. The method comprises the following steps: preparing a sample of the traditional Chinese medicine composition, accurately weighing it, adding an extraction solvent, ultrasonically treating it, cooling it, centrifuging it, and then filtering it with suction; taking the filtrate and spinning it to obtain a residue; adding a solvent to dissolve the residue, fixing the volume, shaking it well, filtering it, and taking a subsequent filtrate to obtain a sample solution; preparing a reference solution: taking six reference substances, including genipin gentiobioside, gardeniaside, magnolamine, and spinosin, to prepare a mixed reference solution; and using HPLC to perform analysis and determination to complete the construction of a fingerprint. The method has strong specificity, high precision, good stability, and strong repeatability, and provides richer information for the intrinsic quality control of traditional Chinese medicine compositions whose raw materials include spinach seeds and gardenia.

The invention relates to the technical field of pharmaceutical analysis, in particular to an HPLC (High Performance Liquid Chromatography) fingerprint spectrum construction method and application of a twelve-ingredient numbness pain liniment. The construction method comprises the following steps: preparing a test solution; preparing a reference substance solution; and HPLC determination. The HPLC fingerprint spectrum construction method of the twelve-ingredient numbness pain liniment is provided for the first time, and the method has the advantages of being high in precision, good in reproducibility, high in stability and good in durability. Meanwhile, the method disclosed by the invention can be used for detecting the contents of three components including polydatin, stilbene glucoside and emodin in the twelve-ingredient numbness pain liniment at one time. Technical support is provided for comprehensively improving the product quality standard of the twelve-ingredient arthralgia pain liniment and optimizing the preparation process, identifying true and false and carrying out follow-up material basic research of the twelve-ingredient arthralgia pain liniment.

The application provides a HPLC-based method for constructing a fingerprint spectrum of Tianyang Qianggu Yin and application thereof, and belongs to the technical field of traditional Chinese medicine detection.The method provided by the application comprises the steps of preparing a test sample solution, preparing a mixed control sample solution, HPLC determination, fingerprint spectrum construction and content determination.The application first establishes a HPLC fingerprint spectrum suitable for water-soluble extracts of Tianyang Qianggu Yin compound, and simultaneously realizes accurate content determination of six components, i.e., neotamanoxin, naringin, alpinumisine A, alpinumisine B, alpinumisine C and icariin.The application has strong specificity, high precision, good repeatability and excellent accuracy.The application provides scientific and controllable technical support for the production quality consistency and efficacy stability of Tianyang Qianggu Yin, and can be used for quality control and batch consistency evaluation of the compound preparation.

The application discloses a kind of kidney Yishen and turbidity releasing mixture HPLC fingerprint quality control method, including the preparation of test solution, HPLC chromatographic conditions and the establishment of fingerprint.The application carries out computer similarity evaluation to the chromatographic peak of different batches of kidney Yishen and turbidity releasing mixture, obtains the common peak of different batches of drugs, and generates HPLC control fingerprint, by comparing with the common peak and similarity in HPLC control fingerprint, the quality of kidney Yishen and turbidity releasing mixture can be comprehensively and quickly evaluated, and the chromatographic peak of each single medicine in prescription is attributed to confirm, can trace the material basis of preparation from raw material source, ensure preparation quality.Further quantitative analysis is carried out to 5 chemical components of kidney Yishen and turbidity releasing mixture, to ensure the stability and uniformity of preparation quality.

The present invention discloses a high performance liquid chromatography fingerprint analysis method for compound Fufangteng capsule and its application, which belongs to the field of chemical analysis technology. A mixed solution of protocatechuic acid, calycosin glucoside, thorn formononetin, calycosin, and formononetin is used as a standard solution, and a methanol extract of compound Fufangteng capsule is used as a test solution for high performance liquid chromatography analysis to establish a compound Fufangteng capsule HPLC fingerprint; wherein, the high performance liquid chromatography analysis uses acetonitrile 0.2% phosphoric acid solution as the mobile phase, the elution mode is gradient elution, and the detection wavelength is 254nm. The method provided by the present invention has good repeatability and high durability, and can be used for compound Fufangteng capsule quality control.

The invention belongs to the technical field of medicine detection, and particularly relates to a quality evaluation method for phlegm and fluid retention pills based on HPLC fingerprint spectrum and multi-index component content determination. The method comprises the following steps: determining the contents of nine active components in the phlegm-drinking pill and constructing an HPLC fingerprint spectrum of the phlegm-drinking pill based on a high performance liquid chromatography-high resolution mass spectrometry technology; wherein the nine active ingredients are sinapine, chlorogenic acid, cryptochlorogenic acid, 3, 6 '-dimustard acyl sucrose, rosmarinic acid, apiose isoliquiritin, isoliquiritin, glycyrrhizic acid and atractylenolide I. The invention further discloses a preparation method of the pharmaceutical composition. According to the method, the fingerprint spectrum and multi-index determination are integrated, a reproducible technical framework is provided for modern quality evaluation of the traditional Chinese medicine compound preparation with lower instrument cost and higher universality, and the industry is promoted to be transformed from'single component control 'to'overall quality management and control'.

The application discloses a method for establishing HPLC fingerprint spectrum of Shengteng Qingbi granules, which comprises preparation of a test sample solution, determination of HPLC chromatographic conditions and production of an HPLC standard fingerprint spectrum. The application also discloses the HPLC standard fingerprint spectrum of Shengteng Qingbi granules obtained by the method, which has 35 common peaks. The quality detection method is simple in operation, high in stability and good in reproducibility, and the obtained spectrum has many characteristic peaks. Through comparison of the common peaks of the standard fingerprint spectrum, the quality of Shengteng Qingbi granules can be comprehensively evaluated and controlled, which is beneficial to accurate evaluation of the internal quality of the preparation and ensures the safety and effectiveness of clinical medication.

The invention relates to the technical field of traditional Chinese medicine analysis, in particular to a construction method of a wine-fried radix berberidis fingerprint spectrum. According to the method, an ellagic acid solution is used as a contrast solution, and HPLC determination is performed on multiple batches of wine-fried scenery test sample solutions, so that the wine-fried scenery fingerprint spectrum is obtained. According to the method, specific chemical components in the wine-fried scenery are detected, so that the fingerprint spectrum is constructed, and the quality control of the wine-fried scenery is better realized. Meanwhile, by establishing the HPLC fingerprint spectrums of 14 batches of wine-fried scenery, the difference conditions of the fingerprint spectrums of different batches of wine-fried scenery are compared, and reference is provided for establishment and quality control of wine-fried scenery quality standards. The wine-roasted landscape fingerprint spectrum constructed by the construction method disclosed by the invention is used as a quality evaluation standard, has the advantages of accuracy and reliability, and can provide reference and basis for wine-roasted landscape research.

The present invention provides a method for detecting the HPLC fingerprint of Atractylodes macrocephala. The present invention provides an Atractylodes macrocephala medicinal material, which uses the above-mentioned detection method to detect the content of four components: atractylodes lactone I, atractylodes lactone II, atractylodes lactone III, and atractylodes ketone. Each gram of Atractylodes macrocephala medicinal material contains 0.21-0.32 mg of atractylodes lactone I, 0.13-0.25 mg of atractylodes lactone II, 0.1-0.19 mg of atractylodes lactone III, and 5.21-14.08 mg of atractylodes ketone. The present invention also provides an HPLC fingerprint of Atractylodes macrocephala and a method for determining the quality of Atractylodes macrocephala medicinal material. The present invention also provides a method for screening quality-marking components of Atractylodes macrocephala medicinal material. The present invention adopts the HPLC-DAD multi-wavelength switching method to establish the HPLC fingerprint of Atractylodes macrocephala, conducts chemometric research at the same time, and determines the contents of atractylodes lactone I, atractylodes lactone II, atractylodes lactone III and atractylodesone. On the one hand, it can comprehensively evaluate the quality of Atractylodes macrocephala, provide a reference for the quality evaluation and control of Atractylodes macrocephala medicinal materials and decoction pieces, and also lay the foundation for the research on its pharmacological substance basis and related compound research; on the other hand, it can provide a reference for the improvement of the quality standard of Atractylodes macrocephala.

The invention discloses a rhizoma belamcandae oral liquid for clearing pestilence and strengthening body resistance and a preparation method of the rhizoma belamcandae oral liquid, and the rhizoma belamcandae oral liquid for clearing pestilence and strengthening body resistance is prepared from the following raw material medicines: rhizoma belamcandae, honeysuckle flower, radix isatidis, folium isatidis, dandelion, herba eupatorii, radix bupleuri, radix astragali seu hedysari, glossy privet fruit and herba artemisiae scopariae. On the basis of the formula of the rhizoma belamcandae antiviral injection, astragalus membranaceus and glossy privet fruit are innovatively added to enhance the effect of supporting the healthy energy, a compound which simultaneously clears away heat and toxic materials and tonifies lung and qi is formed, and by adopting a modern extraction and inclusion technology and combining with a multi-wavelength HPLC fingerprint overall quality control method, the stable content of pharmacodynamic substance groups is ensured; the oral liquid preparation which is safe, convenient to take and suitable for preventing and treating respiratory virus infection is obtained.

The application relates to the technical field of pharmaceutical analysis, and discloses a construction method of almond HPLC (High Performance Liquid Chromatography) fingerprint, which comprises the following steps: step 1, preparing a control reference solution by using tryptophan control; step 2, mixing almond powder and a methanol aqueous solution, and sequentially performing extraction, cooling and filtration treatment to obtain a test solution; and step 3, performing HPLC detection on the test solution and the control reference solution to obtain an almond HPLC fingerprint composed of sample common characteristic peaks. The construction method of the almond HPLC fingerprint provided by the application firstly constructs an almond HPLC fingerprint containing 11 common characteristic peaks of tryptophan, linoleic acid and oleic acid, the fingerprint chromatographic peak is good in shape, stable, excellent in repeatability and high in precision, and the method provides a new technical scheme and technical idea for identification, quality control and quality evaluation of almond and decoction pieces.

The application provides a method for establishing an HPLC fingerprint of Juanbi decoction, and belongs to the technical field of chemical analysis. The method comprises the following steps: S1. preparing a material reference of Juanbi decoction; S2. preparing a single-ingredient medicinal slice sample; S3. preparing a negative sample; S4. preparing a standard solution; and S5. performing chromatographic analysis. The method can help screen out a pharmacodynamic material basis of JBD for resisting RA, and the research provides a solid research basis for establishing a quality evaluation standard for component screening of JBD for resisting RA and development of JBD products.

The present application relates to a kind of fingerprints of radix tetrapanacis and its application in medicinal material quality control, the radix tetrapanacis HPLC fingerprint spectrum established in the present application overcomes the shortcomings of single detection index in prior art, can not reflect the internal quality of medicinal materials, the provided radix tetrapanacis HPLC fingerprint spectrum establishment method is high in precision, good stability and repeatability, by comparing the presence or absence of common peaks in the obtained fingerprint spectrum, the quality of radix tetrapanacis can be effectively characterized, and it has important significance for ensuring clinical efficacy.

The invention discloses a compound lithospermum ointment based on Yunnan lithospermum erythrorhizon and a preparation process thereof, and relates to the technical field of traditional Chinese medicine external preparations, in terms of raw materials, the parameter range of Yunnan lithospermum erythrorhizon and the like is determined, and the quality stability of the raw materials is guaranteed; the extraction process adopts a 15-20 DEG C low-temperature light-shielding oxygen-isolation cold soaking combined pipeline type ultrasonic-assisted extraction process, core parameters are set, the extraction rate of alkannin is increased, and heat-sensitive active ingredients are reserved to the maximum extent; the compound compatibility strictly follows the principle of monarch, minister, assistant and guide, the weight part range of each functional component is defined, and multi-target synergistic interaction is realized; in the aspect of product safety, related parameters such as attributes of raw materials of the whole formula, pH value of the finished product and irritation are strictly limited; an exclusive HPLC fingerprint spectrum quality control system is established, and accurate regulation and control of functional components of a finished product and whole-process quality tracing are achieved; a complete process for preparing a traditional Chinese medicine dyeing product from radix arnebiae decoction dregs is developed, and high-valued cyclic utilization of solid wastes of traditional Chinese medicinal materials is realized.

The invention relates to the technical field of drug detection, in particular to a construction method of a tetrastigma planicaule HPLC fingerprint spectrum. Comprising the following steps: (1) preparation of a test solution: taking a tetrastigma planicaule reference medicinal material, adding methanol, performing ultrasonic treatment, filtering, evaporating filtrate to dryness, adding water into residues for dissolving, extracting with ethyl acetate, combining ethyl acetate solutions, performing vacuum concentration until the solution is dry, fixing the volume with methanol, and filtering to obtain the test solution; (2) taking a resveratrol reference substance, and adding methanol to prepare a resveratrol reference substance solution; and (3) sucking the test solution and the resveratrol reference substance solution, injecting into a liquid chromatograph, and determining. The HPLC fingerprint spectrum established by the invention not only provides comprehensive and scientific technical support for quality evaluation of the tetrastigma planicaule, but also lays a foundation for pharmacological activity research and clinical application development of the tetrastigma planicaule. The method is easy and convenient to operate and good in reproducibility, has wide popularization value and can provide important reference for improvement of a traditional Chinese medicine quality control system.

The invention provides a Tongmai particle HPLC fingerprint establishment method and a fingerprint thereof, and belongs to the technical field of fingerprints, and the method comprises the following steps: preparing a test solution, taking 0.1-0.5 g of Tongmai particle fine powder, precisely weighing, cooling, complementing the reduced weight, and filtering with a 0.45 [mu] m filter membrane to obtain the test solution, comprising the following raw materials: 500 parts of radix salviae miltiorrhizae, 500 parts of rhizoma chuanxiong and 500 parts of radix puerariae. Taking puerarin and salvianolic acid B reference substances, and setting a gradient elution program; performing similarity evaluation by adopting a traditional Chinese medicine chromatographic fingerprint similarity evaluation system; through high performance liquid chromatography-fingerprint analysis, synchronous quantification of multiple components is realized, an HPLC fingerprint method covering water-soluble components at the same time is established, synchronous monitoring of multiple characteristic peaks of Tongmai particles is realized, the limitation of traditional single component detection is solved, and the stability of a production process is objectively quantified through similarity evaluation.

The invention relates to construction of a Qingbai Tongbi capsule fingerprint spectrum and application of the Qingbai Tongbi capsule fingerprint spectrum in drug quality control. The HPLC fingerprint spectrum of the Qingbai Tongbi capsule has 24 characteristic fingerprint chromatographic peaks, wherein the peak 4 is catechin, the peak 10 is sinomenine, the peak 12 is gallic acid, the peak 13 is magnoflorine, the peak 14 is paeoniflorin, the peak 17 is liquiritin, the peak 18 is 1, 2, 3, 4, 6-O-pentagalloylglucose, and the peak 24 is ammonium glycyrrhizinate.

The present application relates to the construction method of Gengnining HPLC fingerprint and its application, which can comprehensively evaluate and control the quality of Gengnining, is beneficial to stabilize the product quality, ensure the stability and uniformity of its inherent quality, ensure the safety and effectiveness of clinical medication, and the technical solution thereof is that the construction method of Gengnining HPLC fingerprint comprises the following steps: 1) preparation of reference solution; 2) preparation of medicinal material solution; 3) preparation of test sample solution; 4) determination of HPLC chromatographic conditions; 5) calculation and determination of the relative retention time and the ratio of relative peak area of 9 main characteristic peaks with large peak area; the method is simple, fast, reproducible, stable, and through the comparison of characteristic peaks in the standard fingerprint, the quality of Gengnining can be comprehensively evaluated and controlled, which is beneficial to stabilize the product quality, ensure the stability and uniformity of its inherent quality, ensure the safety and effectiveness of clinical medication, and is an innovation in the quality detection method of Gengnining product.

The present invention belongs to the technical field of Mongolian medicine preparation and quality inspection, and particularly relates to a Mongolian medicine stir-fried Tribulus terrestris formula granule and a method for constructing its fingerprint and a fingerprint. The Mongolian medicine stir-fried Tribulus terrestris formula granule comprises stir-fried Tribulus terrestris fine powder and pharmaceutical excipients; the fingerprint construction method comprises: extracting the stir-fried Tribulus terrestris formula granule, filtering it, measuring it using ultra-high performance liquid chromatography, constructing a fingerprint, and using "double label and one mass spectrometry" to identify compounds on common peaks. The Mongolian medicine stir-fried Tribulus terrestris formula granule prepared by the present invention has a high forming rate, good fluidity, low hygroscopicity, and good stability. The constructed fingerprint has the advantages of good stability, high precision, and good repeatability. It can fully reflect the intrinsic quality of the stir-fried Tribulus terrestris formula granule, has lower operating costs than completely using liquid chromatography to construct a fingerprint, is faster than ordinary HPLC fingerprint analysis, and consumes less mobile phase solution.

The invention discloses a peony root epidermis chemical component analysis and HPLC fingerprint map quality control detection method, and belongs to the technical field of traditional Chinese medicine quality control. Comprising the following steps: firstly preparing a test sample and a mixed reference substance solution, then establishing an HPLC fingerprint map through HPLC detection, carrying out further chemical component analysis through an HPLC-MS / MS method, identifying 30 characteristic peaks, and finally carrying out quantitative analysis on 22 chemical components with good separation degrees through an HPLC standard curve method. The HPLC fingerprint spectrum and chemical component analysis method of the peony root epidermis is established, simultaneous quantitative detection of 22 components in the peony root epidermis is achieved, the method has the advantages of being high in precision, stability and repeatability, good in recovery rate and the like, a foundation is laid for quality control, development and utilization of the peony root epidermis, and the method is worthy of popularization and application. The problem that peony root epidermis quality standards and component quantitative detection methods are lacked at present is solved.

The invention discloses a method for evaluating flavonoid active ingredients of whole-plant corn silage based on HPLC (High Performance Liquid Chromatography). The method comprises the following steps: S1, collecting and treating a sample; S2, extracting total flavonoids of the sample; S3, preparing before establishment of an HPLC fingerprint spectrum of the active ingredients of the sample; according to the method disclosed by the invention, the chemical characteristic spectrum of the flavonoid active ingredients of the whole corn silage is constructed by adopting a fingerprint spectrum technology and by virtue of multi-dimensional analysis characteristics of the fingerprint spectrum technology, so that the category and content distribution of main effective ingredients in raw materials or finished products can be systematically represented; the whole-plant corn silage flavonoid active ingredients are subjected to integral evaluation and systematic quality control, and the method has the advantages of being good in credibility and high in data accuracy.

The application belongs to the technical field of traditional Chinese medicine detection, and provides a quality evaluation method for asparagus cochinchinensis from different producing areas based on HPLC fingerprint spectrum. The quality evaluation method comprises the following steps: (1) preparing a test sample solution; (2) performing HPLC chromatographic analysis on the test sample solution; (3) preparing test sample solutions of asparagus cochinchinensis samples from different producing areas according to step (1), and performing sample injection under the chromatographic conditions of step (2) to generate different HPLC superimposed spectra of the asparagus cochinchinensis samples and a control fingerprint spectrum; (4) taking the control fingerprint spectrum as a reference, and applying a similarity evaluation system software to derive the similarity of the asparagus cochinchinensis samples from different producing areas and the control fingerprint spectrum. The method can collect asparagus cochinchinensis samples from different producing areas, perform sample extraction and detection, establish HPLC fingerprint spectra of asparagus cochinchinensis from different producing areas, obtain a control fingerprint spectrum, derive the similarity evaluation range of asparagus cochinchinensis from different producing areas, and provide a reference basis for comprehensively evaluating the quality of asparagus cochinchinensis from different producing areas.

The application belongs to the technical field of pharmaceutical analysis, and particularly relates to a method for establishing a high performance liquid chromatography (HPLC) fingerprint of Chuanbei Zhisou mixture. The method comprises the following steps: (1) preparing a test sample solution; (2) preparing a control sample solution; (3) preparing a control medicinal material solution; and (4) HPLC determination. The test sample solution, the control sample solution and the control medicinal material solution are injected into a HPLC instrument under optimized chromatographic conditions, and chromatograms are recorded. The HPLC chromatograms of multiple batches of test sample solutions are analyzed to obtain a control fingerprint of the Chuanbei Zhisou mixture, and the relative retention times of all common peaks are calculated. The method has the advantages of high compound separation degree and good repeatability. The fingerprint provided by the method can be used to determine characteristic compounds in the Chuanbei Zhisou mixture, and can effectively characterize and comprehensively monitor the quality of the Chuanbei Zhisou mixture.