51 results about "Hplc fingerprint" patented technology

The application discloses an evaluation method for distinguishing different quality of momordica grosvenori of different origins. The application adopts high performance liquid chromatography to establish the fingerprint of momordica grosvenori, carries out gradient elution by taking water-acetonitrile as a mobile phase, and detects the wavelength of 203 nm to obtain the HPLC fingerprint of momordica grosvenori; the obtained chromatogram is introduced into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system to generate a control fingerprint, different origins of momordica grosvenori are distinguished through similarity comparison and / or chemical pattern recognition analysis; and chemical markers with VIP>1 such as 5-hydroxymethyl furfural, momordica grosvenori flavonoid, momordica grosvenori saponin V and momordica grosvenori saponin IIIA2 are screened. The application can effectively distinguish two main origins of green skin fruit and red hair fruit, overcomes the defects that traditional trait identification is subjective and it is difficult to distinguish similar appearance samples, and provides an objective, accurate and stable technical means for origin identification, quality consistency evaluation and authenticity identification of momordica grosvenori.

The invention relates to and provides a fingerprint spectrum of musk-chuan NaoLiantong granules and a detection method for quantitative analysis of multiple components by single marker. The fingerprint spectrum detection method has the characteristics of simplicity and convenience in operation and good stability and reproducibility. 13 common peaks exist in an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of 15 batches of musk-chuan naoeutong granules, and 7 components in the fingerprint spectrum, namely geniposidic acid, chlorogenic acid, cowherb seed flavonoid glycoside, ferulic acid, senkyunolide I, salvianolic acid B and tanshinone IIA, are identified. The similarity of the 15 batches of the musk and chuan naoliantong granules is 0.961-1. According to the fingerprint spectrum detection method, 13 representative common characteristic peaks are determined so as to comprehensively reflect the internal quality characteristics of the musk-chuan naoliantong preparation.

The application provides a method for establishing HPLC fingerprint of Changnyle capsule (I), which is characterized in that 16 active components including gastrodin, danshensu, quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiopicroside, 3'-hydroxy puerarin, puerarin, 3'-methoxy puerarin, puerarin apioside, paeoniflorin, daidzin, rosmarinic acid, shikonin, isoquercitrin, ononin, daidzein, salvianolic acid B and calycosin are used as reference substances, HPLC is adopted, methanol-acetonitrile-0.5vol% phosphoric acid aqueous solution is used as a mobile phase, different batches of samples are detected, and finally the fingerprint of Changnyle capsule (I) is obtained. The fingerprint can comprehensively represent the main components in Changnyle capsule (I), thereby being beneficial to the quality control of Changnyle capsule (I).

The invention relates to the technical field of pharmaceutical analysis, in particular to an HPLC (High Performance Liquid Chromatography) fingerprint spectrum construction method and application of a twelve-ingredient numbness pain liniment. The construction method comprises the following steps: preparing a test solution; preparing a reference substance solution; and HPLC determination. The HPLC fingerprint spectrum construction method of the twelve-ingredient numbness pain liniment is provided for the first time, and the method has the advantages of being high in precision, good in reproducibility, high in stability and good in durability. Meanwhile, the method disclosed by the invention can be used for detecting the contents of three components including polydatin, stilbene glucoside and emodin in the twelve-ingredient numbness pain liniment at one time. Technical support is provided for comprehensively improving the product quality standard of the twelve-ingredient arthralgia pain liniment and optimizing the preparation process, identifying true and false and carrying out follow-up material basic research of the twelve-ingredient arthralgia pain liniment.

The application provides a HPLC-based method for constructing a fingerprint spectrum of Tianyang Qianggu Yin and application thereof, and belongs to the technical field of traditional Chinese medicine detection.The method provided by the application comprises the steps of preparing a test sample solution, preparing a mixed control sample solution, HPLC determination, fingerprint spectrum construction and content determination.The application first establishes a HPLC fingerprint spectrum suitable for water-soluble extracts of Tianyang Qianggu Yin compound, and simultaneously realizes accurate content determination of six components, i.e., neotamanoxin, naringin, alpinumisine A, alpinumisine B, alpinumisine C and icariin.The application has strong specificity, high precision, good repeatability and excellent accuracy.The application provides scientific and controllable technical support for the production quality consistency and efficacy stability of Tianyang Qianggu Yin, and can be used for quality control and batch consistency evaluation of the compound preparation.

The application discloses a kind of kidney Yishen and turbidity releasing mixture HPLC fingerprint quality control method, including the preparation of test solution, HPLC chromatographic conditions and the establishment of fingerprint.The application carries out computer similarity evaluation to the chromatographic peak of different batches of kidney Yishen and turbidity releasing mixture, obtains the common peak of different batches of drugs, and generates HPLC control fingerprint, by comparing with the common peak and similarity in HPLC control fingerprint, the quality of kidney Yishen and turbidity releasing mixture can be comprehensively and quickly evaluated, and the chromatographic peak of each single medicine in prescription is attributed to confirm, can trace the material basis of preparation from raw material source, ensure preparation quality.Further quantitative analysis is carried out to 5 chemical components of kidney Yishen and turbidity releasing mixture, to ensure the stability and uniformity of preparation quality.

The application discloses a method for establishing HPLC fingerprint spectrum of Shengteng Qingbi granules, which comprises preparation of a test sample solution, determination of HPLC chromatographic conditions and production of an HPLC standard fingerprint spectrum. The application also discloses the HPLC standard fingerprint spectrum of Shengteng Qingbi granules obtained by the method, which has 35 common peaks. The quality detection method is simple in operation, high in stability and good in reproducibility, and the obtained spectrum has many characteristic peaks. Through comparison of the common peaks of the standard fingerprint spectrum, the quality of Shengteng Qingbi granules can be comprehensively evaluated and controlled, which is beneficial to accurate evaluation of the internal quality of the preparation and ensures the safety and effectiveness of clinical medication.

The invention relates to the technical field of traditional Chinese medicine analysis, in particular to a construction method of a wine-fried radix berberidis fingerprint spectrum. According to the method, an ellagic acid solution is used as a contrast solution, and HPLC determination is performed on multiple batches of wine-fried scenery test sample solutions, so that the wine-fried scenery fingerprint spectrum is obtained. According to the method, specific chemical components in the wine-fried scenery are detected, so that the fingerprint spectrum is constructed, and the quality control of the wine-fried scenery is better realized. Meanwhile, by establishing the HPLC fingerprint spectrums of 14 batches of wine-fried scenery, the difference conditions of the fingerprint spectrums of different batches of wine-fried scenery are compared, and reference is provided for establishment and quality control of wine-fried scenery quality standards. The wine-roasted landscape fingerprint spectrum constructed by the construction method disclosed by the invention is used as a quality evaluation standard, has the advantages of accuracy and reliability, and can provide reference and basis for wine-roasted landscape research.

The invention discloses a rhizoma belamcandae oral liquid for clearing pestilence and strengthening body resistance and a preparation method of the rhizoma belamcandae oral liquid, and the rhizoma belamcandae oral liquid for clearing pestilence and strengthening body resistance is prepared from the following raw material medicines: rhizoma belamcandae, honeysuckle flower, radix isatidis, folium isatidis, dandelion, herba eupatorii, radix bupleuri, radix astragali seu hedysari, glossy privet fruit and herba artemisiae scopariae. On the basis of the formula of the rhizoma belamcandae antiviral injection, astragalus membranaceus and glossy privet fruit are innovatively added to enhance the effect of supporting the healthy energy, a compound which simultaneously clears away heat and toxic materials and tonifies lung and qi is formed, and by adopting a modern extraction and inclusion technology and combining with a multi-wavelength HPLC fingerprint overall quality control method, the stable content of pharmacodynamic substance groups is ensured; the oral liquid preparation which is safe, convenient to take and suitable for preventing and treating respiratory virus infection is obtained.

The application relates to the technical field of pharmaceutical analysis, and discloses a construction method of almond HPLC (High Performance Liquid Chromatography) fingerprint, which comprises the following steps: step 1, preparing a control reference solution by using tryptophan control; step 2, mixing almond powder and a methanol aqueous solution, and sequentially performing extraction, cooling and filtration treatment to obtain a test solution; and step 3, performing HPLC detection on the test solution and the control reference solution to obtain an almond HPLC fingerprint composed of sample common characteristic peaks. The construction method of the almond HPLC fingerprint provided by the application firstly constructs an almond HPLC fingerprint containing 11 common characteristic peaks of tryptophan, linoleic acid and oleic acid, the fingerprint chromatographic peak is good in shape, stable, excellent in repeatability and high in precision, and the method provides a new technical scheme and technical idea for identification, quality control and quality evaluation of almond and decoction pieces.

The application provides a method for establishing an HPLC fingerprint of Juanbi decoction, and belongs to the technical field of chemical analysis. The method comprises the following steps: S1. preparing a material reference of Juanbi decoction; S2. preparing a single-ingredient medicinal slice sample; S3. preparing a negative sample; S4. preparing a standard solution; and S5. performing chromatographic analysis. The method can help screen out a pharmacodynamic material basis of JBD for resisting RA, and the research provides a solid research basis for establishing a quality evaluation standard for component screening of JBD for resisting RA and development of JBD products.

The present application relates to a kind of fingerprints of radix tetrapanacis and its application in medicinal material quality control, the radix tetrapanacis HPLC fingerprint spectrum established in the present application overcomes the shortcomings of single detection index in prior art, can not reflect the internal quality of medicinal materials, the provided radix tetrapanacis HPLC fingerprint spectrum establishment method is high in precision, good stability and repeatability, by comparing the presence or absence of common peaks in the obtained fingerprint spectrum, the quality of radix tetrapanacis can be effectively characterized, and it has important significance for ensuring clinical efficacy.

The invention discloses a compound lithospermum ointment based on Yunnan lithospermum erythrorhizon and a preparation process thereof, and relates to the technical field of traditional Chinese medicine external preparations, in terms of raw materials, the parameter range of Yunnan lithospermum erythrorhizon and the like is determined, and the quality stability of the raw materials is guaranteed; the extraction process adopts a 15-20 DEG C low-temperature light-shielding oxygen-isolation cold soaking combined pipeline type ultrasonic-assisted extraction process, core parameters are set, the extraction rate of alkannin is increased, and heat-sensitive active ingredients are reserved to the maximum extent; the compound compatibility strictly follows the principle of monarch, minister, assistant and guide, the weight part range of each functional component is defined, and multi-target synergistic interaction is realized; in the aspect of product safety, related parameters such as attributes of raw materials of the whole formula, pH value of the finished product and irritation are strictly limited; an exclusive HPLC fingerprint spectrum quality control system is established, and accurate regulation and control of functional components of a finished product and whole-process quality tracing are achieved; a complete process for preparing a traditional Chinese medicine dyeing product from radix arnebiae decoction dregs is developed, and high-valued cyclic utilization of solid wastes of traditional Chinese medicinal materials is realized.

The invention relates to the technical field of drug detection, in particular to a construction method of a tetrastigma planicaule HPLC fingerprint spectrum. Comprising the following steps: (1) preparation of a test solution: taking a tetrastigma planicaule reference medicinal material, adding methanol, performing ultrasonic treatment, filtering, evaporating filtrate to dryness, adding water into residues for dissolving, extracting with ethyl acetate, combining ethyl acetate solutions, performing vacuum concentration until the solution is dry, fixing the volume with methanol, and filtering to obtain the test solution; (2) taking a resveratrol reference substance, and adding methanol to prepare a resveratrol reference substance solution; and (3) sucking the test solution and the resveratrol reference substance solution, injecting into a liquid chromatograph, and determining. The HPLC fingerprint spectrum established by the invention not only provides comprehensive and scientific technical support for quality evaluation of the tetrastigma planicaule, but also lays a foundation for pharmacological activity research and clinical application development of the tetrastigma planicaule. The method is easy and convenient to operate and good in reproducibility, has wide popularization value and can provide important reference for improvement of a traditional Chinese medicine quality control system.

The present application relates to the construction method of Gengnining HPLC fingerprint and its application, which can comprehensively evaluate and control the quality of Gengnining, is beneficial to stabilize the product quality, ensure the stability and uniformity of its inherent quality, ensure the safety and effectiveness of clinical medication, and the technical solution thereof is that the construction method of Gengnining HPLC fingerprint comprises the following steps: 1) preparation of reference solution; 2) preparation of medicinal material solution; 3) preparation of test sample solution; 4) determination of HPLC chromatographic conditions; 5) calculation and determination of the relative retention time and the ratio of relative peak area of 9 main characteristic peaks with large peak area; the method is simple, fast, reproducible, stable, and through the comparison of characteristic peaks in the standard fingerprint, the quality of Gengnining can be comprehensively evaluated and controlled, which is beneficial to stabilize the product quality, ensure the stability and uniformity of its inherent quality, ensure the safety and effectiveness of clinical medication, and is an innovation in the quality detection method of Gengnining product.

The invention discloses a method for evaluating flavonoid active ingredients of whole-plant corn silage based on HPLC (High Performance Liquid Chromatography). The method comprises the following steps: S1, collecting and treating a sample; S2, extracting total flavonoids of the sample; S3, preparing before establishment of an HPLC fingerprint spectrum of the active ingredients of the sample; according to the method disclosed by the invention, the chemical characteristic spectrum of the flavonoid active ingredients of the whole corn silage is constructed by adopting a fingerprint spectrum technology and by virtue of multi-dimensional analysis characteristics of the fingerprint spectrum technology, so that the category and content distribution of main effective ingredients in raw materials or finished products can be systematically represented; the whole-plant corn silage flavonoid active ingredients are subjected to integral evaluation and systematic quality control, and the method has the advantages of being good in credibility and high in data accuracy.

The application belongs to the technical field of pharmaceutical analysis, and particularly relates to a method for establishing a high performance liquid chromatography (HPLC) fingerprint of Chuanbei Zhisou mixture. The method comprises the following steps: (1) preparing a test sample solution; (2) preparing a control sample solution; (3) preparing a control medicinal material solution; and (4) HPLC determination. The test sample solution, the control sample solution and the control medicinal material solution are injected into a HPLC instrument under optimized chromatographic conditions, and chromatograms are recorded. The HPLC chromatograms of multiple batches of test sample solutions are analyzed to obtain a control fingerprint of the Chuanbei Zhisou mixture, and the relative retention times of all common peaks are calculated. The method has the advantages of high compound separation degree and good repeatability. The fingerprint provided by the method can be used to determine characteristic compounds in the Chuanbei Zhisou mixture, and can effectively characterize and comprehensively monitor the quality of the Chuanbei Zhisou mixture.

The present application relates to a kind of ginseng Yi Yuan granules fingerprint detection method.By this method, the quality of ginseng Yi Yuan granules can be effectively characterized, which is beneficial to the overall monitoring of product quality stability, consistency, controllability.The ginseng Yi Yuan granules fingerprint constructed by the present application has 21 common peaks, and contains effective components belonging to six medicinal materials such as white peony root, white atractylodes, licorice, astragalus, caulis Bambusae and ginseng, effectively characterizing the quality of ginseng Yi Yuan granules, which is beneficial to the overall monitoring of product quality.The present application has the characteristics of convenience, speed, stability, high precision and good reproducibility, and can more comprehensively, objectively and scientifically evaluate the quality of ginseng Yi Yuan granules, thereby ensuring the safety and effectiveness of clinical medication.

This invention proposes an analytical method for evaluating the quality of red yeast rice based on HPLC fingerprinting and multi-component quantitative analysis, belonging to the field of quality control technology for red yeast rice and related products. The method includes: taking a red yeast rice sample, ultrasonically extracting it with 50% methanol, centrifuging, and filtering to prepare a test solution; performing detection using high-performance liquid chromatography; constructing a red yeast rice fingerprint chromatogram based on the detection results, and simultaneously determining the content of six active ingredients: ankaflavin, citrinin, lactone-type mevastatin, lactone-type lovastatin, acid mevastatin, and acid lovastatin; and combining chemical pattern recognition methods to perform discriminant analysis on red yeast rice samples from different sources to evaluate the quality of the red yeast rice. This invention achieves an organic combination of simultaneous quantitative analysis of multiple components in red yeast rice and overall quality evaluation based on fingerprint chromatograms. Methodological validation shows that this method has good precision, repeatability, and stability, and is accurate and reliable, suitable for the quality control of red yeast rice raw materials and health foods and pharmaceuticals containing red yeast rice.

The application discloses a HPLC fingerprint spectrum establishing and content determination method of Zha Dihu, and belongs to the technical field of drug chemical component analysis. The application establishes a HPLC fingerprint spectrum, confirms that six common peaks in test sample solutions of 14 batches are p-hydroxybenzoic acid, vanillic acid, p-hydroxybenzaldehyde, shatavari glycoside, paeoniflorin and p-hydroxycinnamic acid, adopts SPSS 27 and SIMCA14.1 analysis software, carries out cluster analysis, principal component analysis and orthogonal partial least square discriminant analysis on peak areas of the six identified common peaks in the fingerprint spectrum of the Zha Dihu medicinal materials of the 14 batches, and carries out main component content determination. The chemical component composition of the Zha Dihu is determined, the characteristic components are revealed, and the main components are quantitatively analyzed, so as to provide a scientific basis for quality evaluation, efficacy research and subsequent development and utilization of the Zha Dihu.

The application discloses a method for evaluating the quality of Qingxin Chenxiang Baba Pills, which comprises the following steps: (1) establishing a fingerprint of each QCBP test solution and calibrating common peaks; (2) evaluating and analyzing the multi-dimensional quality differences of several QCBP test samples; (3) if there are quality differences, screening out key factors causing greater differences between the QCBP test samples from the common peaks of the calibrated QCBP test solution; and (4) determining the contents of each component causing the quality differences of the samples. The application is based on the component analysis of HPLC-Q-Exactive-MS technology, the establishment of HPLC fingerprint and the quantitative evaluation of HPLC-MS difference components, and provides a reference for the quality evaluation system of commercially available QCBP.

The invention discloses an HPLC (High Performance Liquid Chromatography) fingerprint spectrum detection method of an acid-clearing tablet, and belongs to the technical field of medicine quality detection. The method comprises the following steps: S1, firstly taking acid-clearing tablet powder, adding a methanol solution as an extraction solvent, weighing, then carrying out ultrasonic treatment and cooling, weighing again, finally complementing the reduced weight with the methanol solution, uniformly shaking, filtering, and taking a subsequent filtrate as a test solution; s2, preparing a reference substance solution; and S3, sucking the test solution and the reference solution, carrying out high performance liquid chromatography determination, and establishing the HPLC fingerprint spectrum of the Xiaoshan tablet. According to the invention, the HPLC fingerprint spectrum of the Xianshu tablets is established, qualitative analysis of the Xianshu tablets is realized, a feasible method is provided for comprehensive quality evaluation of the Xianshu tablets, and a foundation is laid for basic research of subsequent pharmacodynamic substances of the Xianshu tablets; the method for comprehensively evaluating the quality of the acid-clearing tablets through the fingerprint spectrum is simple and convenient to operate, stable and reliable in result and high in repeatability, and can be used for comprehensively evaluating the overall quality of the acid-clearing tablets.

This invention relates to the field of quality control and analytical chemistry technology for traditional Chinese medicine, and discloses a quality evaluation system for Ganoderma lucidum (reishi mushroom) based on HPLC fingerprinting and metrology. This system is a software platform integrating HPLC instrument control, data processing, modeling analysis, and result output. Its system architecture includes: a data interface and acquisition layer; a core data processing and analysis layer; a knowledge base and data management layer; an intelligent decision-making and report generation layer; and a user interaction and application layer. This quality evaluation system for Ganoderma lucidum (reishi mushroom) based on HPLC fingerprinting and metrology designs and constructs an integrated system with hardware and software collaboration and modular functions. The system seamlessly connects the entire process, including sample information management, automatic HPLC data acquisition, fingerprint standardization processing, automatic similarity and chemometric analysis, intelligent screening of characteristic markers, comprehensive evaluation, and report generation, achieving "one-click" automated processing from raw data to evaluation conclusions.

The application discloses a method for constructing a HPLC fingerprint of small-leaf three-point gold, and belongs to the technical field of medicinal material analysis. The method comprises the following technical steps: (1) preparation of a test sample solution; (2) preparation of a mixed control sample solution; (3) high performance liquid chromatography detection: the test sample solution and the mixed control sample solution are injected into a high performance liquid chromatograph for detection, and a chromatogram is recorded; (4) establishment of a fingerprint: the chromatogram is introduced into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, common peaks are matched, a control fingerprint is generated, and the common peaks are identified. The method has the advantages of good precision, repeatability and stability, etc., can comprehensively reflect the overall characteristics of the small-leaf three-point gold, rapidly, comprehensively and accurately evaluates the overall quality of the small-leaf three-point gold, and effectively guarantees the overall quality of medicinal materials.

The application discloses a method for establishing HPLC fingerprint spectrum of Lianhuaqingwen capsules based on chemical identification method, and is characterized in that the method adopts high performance liquid chromatography and specifically comprises the following steps: 1) preparation of reference solution: precisely take new chlorogenic acid, quercitrin, forsythia glycoside, amygdalin, emodin, chrysophanol, chlorogenic acid, forsythia glycoside A, rhein, glycyrrhizic acid reference, and add methanol to prepare a reference solution; 2) preparation of test sample solution: 3) chromatographic conditions: the mobile phase is 0.1% phosphoric acid aqueous solution (A)-acetonitrile (B), gradient elution; the detection wavelength is 207 nm, the column temperature is 30 DEG C, and the flow rate is 1.0 mL·min ‑1 ; 4) determination: precisely take the test sample solution and inject it into a high performance liquid chromatograph, and the HPLC fingerprint spectrum is obtained by HPLC determination. The HPLC fingerprint spectrum established by the application is helpful for further improving the quality evaluation system of Lianhuaqingwen capsules and can provide a reference for the quality control of Lianhuaqingwen capsules.

This invention discloses a method for establishing an HPLC fingerprint of a traditional Chinese medicine composition. The method includes the preparation of a reference standard and a test solution, the determination of HPLC chromatographic conditions, and the preparation of an HPLC standard fingerprint. The HPLC standard fingerprint of the traditional Chinese medicine composition obtained by this method is also disclosed. This fingerprint has 20 peaks, with 14 common peaks identified by the reference standard. The method is simple, stable, and reproducible, and the obtained chromatogram has comprehensive characteristic peaks. By comparing the common peaks of the standard fingerprint, the quality of the traditional Chinese medicine composition can be comprehensively evaluated and controlled, which is beneficial for accurately assessing the intrinsic quality of the preparation and ensuring its safety and efficacy.

The invention discloses a method for establishing an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of a heart-soothing and lipid-lowering tablet, which comprises the following steps: preparing a reference substance solution and a test sample solution, determining HPLC chromatographic conditions and preparing an HPLC standard fingerprint spectrum. Meanwhile, the invention discloses an HPLC standard fingerprint spectrum of the heart-soothing and lipid-lowering tablet obtained by the method, the fingerprint spectrum has 15 peaks, and 6 common peaks are identified through a reference substance. The method is simple, convenient and good in stability and reproducibility, obtained spectrum characteristic peaks are comprehensive, the quality of the heart-soothing and lipid-lowering tablets can be comprehensively evaluated and controlled through comparison of standard fingerprint spectrum common peaks, the internal quality of the preparation can be accurately evaluated, and the safety and effectiveness of clinical medication are ensured.

The invention discloses an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of a notopterygium root medicinal material and a quantitative analysis of multi-components by single marker, and aims to solve the problems that the current notopterygium root medicinal material base original chemical characteristics are not obvious, the detection depends on a high-cost reference substance, the pharmacodynamic substances cannot be comprehensively represented and the like. According to the method, a comprehensive fingerprint spectrum is established, the overall chemical component outline of the medicinal material is captured, and two base raw medicinal materials can be distinguished; the quantitative analysis of multiple components is realized by applying a quantitative analysis of multiple components by single marker and taking notopterygium alcohol as an internal standard substance, so that the detection cost is remarkably reduced and the efficiency is improved; in combination with chemical mode recognition analysis such as principal component analysis, hierarchical clustering analysis and orthogonal partial least square discriminant analysis, screening characteristic components influencing quality; and objective weight distribution and quality sorting are carried out by using an entropy weight approximation ideal solution sorting method, the advantages and disadvantages of samples of different producing areas and batches are comprehensively evaluated, the method comprehensively improves the controllability and accuracy of the quality of the medicinal materials, effectively distinguishes original differences, and provides a reliable scientific framework for standardization and sustainable utilization of traditional Chinese medicine resources.

The application discloses a kind of HPLC fingerprint detection methods of traditional Chinese medicine, the traditional Chinese medicine is made of ten traditional Chinese medicinal materials such as radix codonopsis, atractylodes, poria, licorice, angelica, chuanxiong, white peony root, prepared rehmannia, astragalus, cassia, with the effect of tonifying qi and blood, the fingerprint established in the application includes 11 common characteristic peaks, respectively belong to ten medicinal materials, so it can identify all ten medicinal materials in traditional Chinese medicine, and also identifies 5 components therein, which are glycyrrhizin, ammonium glycyrrhizinate, verbascoside, calycosin-7-glucoside and astragaloside A respectively, so the application can more comprehensively control drug quality, solve the phenomenon of fake and inferior drugs, and ensure the safety and effectiveness of clinical use.

The invention discloses an HPLC (High Performance Liquid Chromatography) fingerprint spectrum detection method for hangover-alleviating and liver-protecting tablets, and belongs to the technical field of medicine detection. The method comprises the following steps: firstly preparing a test sample and a mixed reference substance solution, then establishing an HPLC fingerprint map through HPLC detection, further identifying the components through an HPLC-MS / MS method, and identifying that 43 components contain 25 common peaks. The HPLC fingerprint spectrum and the HPLC-MS / MS component analysis method of the hangover alleviating and liver protecting tablets are established, the advantages of high precision, high stability, high repeatability, good recovery rate and the like are achieved, a foundation is laid for efficient and accurate quality control and detection of the hangover alleviating and liver protecting tablets, and the problem that the hangover alleviating and liver protecting tablets lack an effective quality detection and control method is solved.