Establishment of HPLC (High Performance Liquid Chromatography) fingerprint spectrum of rabdosia lophanthide medicinal materials and fingerprint spectrum of of rabdosia lophanthide medicinal materials

A technology of fingerprints and herbal materials, which is applied in the field of standard fingerprints and the construction of high-performance liquid chromatography fingerprints of Xihuang herbal materials, can solve the problems of complex sources of traditional Chinese medicine Xihuangcao, similar plant shapes and difficult identification, etc. The method is simple, accurate, and has good reproducibility.

Active Publication Date: 2014-05-07
DONGGUAN MATHEMATICAL ENG ACAD OF CHINESE MEDICINE GUANGZHOU UNIV OF CHINESE MEDICINE
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The source of the traditional Chinese medicine Xihuangcao is complicated, the same name is different, the variety is con

Method used

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  • Establishment of HPLC (High Performance Liquid Chromatography) fingerprint spectrum of rabdosia lophanthide medicinal materials and fingerprint spectrum of of rabdosia lophanthide medicinal materials
  • Establishment of HPLC (High Performance Liquid Chromatography) fingerprint spectrum of rabdosia lophanthide medicinal materials and fingerprint spectrum of of rabdosia lophanthide medicinal materials
  • Establishment of HPLC (High Performance Liquid Chromatography) fingerprint spectrum of rabdosia lophanthide medicinal materials and fingerprint spectrum of of rabdosia lophanthide medicinal materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: Detecting the fingerprints of different varieties of Xihuang herbal medicinal materials

[0046] 1. Instruments and reagents

[0047] 1.1 Ultrapure water machine for instrument laboratory (Chongqing Lidi Modern Water Technology Equipment Co., Ltd.); household universal pulverizer FZ-230 (Wenling Muyu Baile Machine Tool Factory); ultrasonic cleaner (ULTRASONIC; 360kw, 250kHz) ; Electric constant temperature water bath DK-98-11A; Electric blast drying oven type 101-1AB; FEJ-200 electronic scale (d=0.1g); 1 / 10,000 electronic balance (Sartorius BP110s, German company Sartorius); 100,000 One-fifth electronic balance (Sartorius CP2250, Germany sartorius company); Japan Shimadzu high performance liquid chromatograph 1 (SIL-20A prominence AUTO SAMPLER, LC-20AT prominence LIQUID CHROMATOGRAPH, DGU-20A5prominence DEGASSER, SPD-M20A prominence DIODE ARRAY DETECTOR , CTO-20A prominence column oven); Shimadzu HPLC UV-1 chromatograph 2 (SIL-20A prominence Auto sampler, SP...

Embodiment 2

[0057] Example 2: Determination and data analysis of fingerprints of 12 batches of Xihuangcao (Kuxihuang) medicinal materials

[0058] 12 batches of Xihuangcao medicinal materials were taken and detected according to the conditions of Example 1, and the HPLC spectra of 12 batches of samples were obtained.

[0059] Through the comparison of 10 batches of HPLC chromatograms, the similarity evaluation is carried out to determine the common peaks of its characteristics: import the chromatograms of 12 batches of Xihuangcao into the "Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System (Chinese Pharmacopoeia Commission 2004A Edition)", select No. 15 peak (rosmarinic acid) as a reference, as figure 2 , 21 common peaks were identified. The comparison spectrum R is automatically generated by using the median method to automatically match, and the similarity of each medicinal material is obtained. The similarity of 12 batches of Xihuangcao. See Table 1.

[0060...

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Abstract

The invention discloses establishment of an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of rabdosia lophanthide medicinal materials and a fingerprint spectrum of the rabdosia lophanthide medicinal materials, relates to a quality control method of traditional Chinese medicine medicinal materials, and particularly relates to a method for detecting an extracting solution of the rabdosia lophanthide medicinal materials by establishing the HPLC fingerprint spectrum. The method comprises the following steps: preparing a comparison product solution; selecting an extracting solvent and an extracting method; preparing a sample solution; inspecting chromatographic conditions, namely carrying out gradient elution by taking octadecyl bonded silica gel as filler of a chromatographic column, wherein a gradient elution solution is composed of acetonitrile-phosphoric acid with a flowing phase being 0.1%-0.3%, the column temperature is 25-40 DEG C, the ultraviolet detection wavelength is 230-270nm and the time is 100-120min; determining the HPLC fingerprint spectrums of the different types of rabdosia lophanthide medicinal materials; and marking a characteristic peak and selecting a reference peak. By adopting the method disclosed by the invention, the dispute of using rabdosia lophanthide as a medicine base source is solved. Methods including common peak comparison, clustering analysis, characteristic peak analysis and the like of the fingerprint spectrum are comprehensively utilized and are used for identifying the three types of HPLC fingerprint spectrums, so as to realize the base source identification of the rabdosia lophanthide medicinal materials, divide production area classification and provide evidences on quality control and accurate clinical utilization of the rabdosia lophanthide medicinal materials.

Description

technical field [0001] The invention relates to a quality control method of traditional Chinese medicinal materials, in particular to a construction method and a standard fingerprint of Xihuang herbal medicinal materials by high-performance liquid chromatography. Background technique [0002] Xihuang prairie plant is the dry aerial part of Isodon lophanthoides (Buch.-Ham.ex D.Don) H.Hara. It is mainly produced in Hunan, Hubei, Sichuan, Yunnan, Jiangxi, Guangdong, Guangxi, Fujian and other provinces and regions south of the Yangtze River. Bitter, sweet, cold in nature, returns to the liver and gallbladder meridian, has the effects of clearing heat and dampness, reducing jaundice, cooling blood and dissipating blood stasis, and is used to treat damp-heat jaundice, damp-heat diarrhea, traumatic pain, acute jaundice hepatitis, acute Cholecystitis, dysentery, enteritis and other diseases. [0003] Xihuangcao is a traditional herbal medicine in South China. As a liver-clearing ...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 赖小平黄松蒋东旭
Owner DONGGUAN MATHEMATICAL ENG ACAD OF CHINESE MEDICINE GUANGZHOU UNIV OF CHINESE MEDICINE
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