The invention belongs to the technical field of agricultural
biology, and relates to a rapid propagation method of pipewort seedlings, which comprises the following steps: disinfecting robust pipewort plants with 84
disinfectant, stripping stem tips, carrying out induced differentiation to form adventitious
bud clusters,
cutting the adventitious
bud clusters into small blocks, carrying out subculture multiplication, carrying out strong
seedling culture,
cutting single plants, and carrying out rooting culture to obtain complete plants.
Transplanting to a culture medium after
seedling hardening, spraying sufficient root fixing water, and carrying out water and
fertilizer management and
pest control measures, so as to obtain the
tissue culture seedlings of the eriocaulon chinense. According to the method, the pipewort seedlings are propagated in a
tissue culture mode, the defects of
disease and pest propagation caused by division propagation, low
sowing propagation
germination rate, low efficiency and the like are overcome, stable inheritance of excellent characters of stock plants is kept, the method is not limited by seasons and weather conditions, production can be conducted all the year round, and the method is easy to implement, easy to operate, low in input cost and suitable for large-scale popularization and application. The
seedling forming speed is high, and a technical foundation is laid for effective utilization of the Eriocaulum arvense resources.