The present invention relates to a synthesis method of (7Z,11Z,13E)-hexadecatrienal as main constituent of citrus leaf-miner sex pheromone. 7-Bromo-(4Z,6E)-heptadienal and 4-bromo-1-butenol are used as start raw materials, the 4-bromo-1-butenol is coupled with allylmagnesium bromide to obtain 6-hepten-1-ol and then the 6-hepten-1-ol is brominated and salified with triphenylphosphine to obtain 1-bromo-(1E,3Z,7Z,13)-tetradecatetraene, and then the 1-bromo-(1E,3Z,7Z,13)-tetradecatetraen is subjected to Kumada coupling to obtain (1,7Z,11Z,13E)-hexadecatetraene, and then the (1,7Z,11Z,13E)-hexadecatetraene is subjected to hydroboration-oxidation reaction to obtain (7Z,11Z,13E)-hexadecatrienol, and finally the (7Z,11Z,13E)-hexadecatrienol is oxidized into (7Z,11Z,13E)-hexadecatrienal by pyridinium chlorochromate. The synthesis method of (7Z,11Z,13E)-hexadecatrienal has advantages of cheap and easily obtained raw materials, simple synthesis path, mild reaction conditions, high yield, low cost, convenient and safe operation.