70 results about "Ostrinia" patented technology

The subject invention relates in part to stacking Cry1Be toxins along with Cry1Fa toxins to prevent insects from developing resistance towards either toxin by itself. As discussed in more detail herein, the subject pair of proteins is a particularly advantageous combination, as no other pair of proteins is known to provide high levels of control and non-cross-resistant activity against both Spodoptera frugiperda (FAW) and Ostrinia nubilalis (ECB) insects. This dual, non-cross-resistant activity is also advantageous because it can reduce the number of proteins / genes needed to target these insects with multiple, non-cross-resistant proteins. This can reduce or eliminate the need for refuge acreage. Accordingly, the subject invention also relates generally to using four genes to provide three proteins for non-cross-resistant control of a first insect, and three proteins for non-cross-resistant control of a second insect. In preferred embodiments, the targeted insects are FAW and ECB.

The invention belongs to the field of pesticide, which mainly relates to an ultralow-volume solvent containing pleocidin or spinetoram. The ultralow-volume solvent is an active component I or an active ingredient compounded by the active component I or an active component II, and the balance is solvent and accessory ingredient, wherein the active component I is pleocidin or spinetoram; and the active component II is insecticide of the other type of action mechanism. The ultralow volume solvent is simple to process and has the advantages of water conservation, high work efficiency, long efficacy continuing period, small possibility of generating resistance to drug for a target, small environment pollution, synergy and the like. Ultralow volume spraying, low-volume spraying or low-volume electrostatic spraying is applied to effectively prevent and control various pests, such as Cnaphalocrocis medinalis, Chilo suppressalis, rice planthopper, Ostrinia nubilalis, plutella xylostella, cabbage caterpillar, Spodoptera litura, Spodoptera exigua, aphids, Lesser green leaf hopper, Icerya purchase, aleyrodid, Thrip, stinkbug, acridid, flea beetle, leafminer, looper and the like.

The invention relates to preparation methods and applications of beauveria bassiana(Bals.-Criv.)Vuill and a bactericide thereof. Bassiana(Bals.-Criv.)Vuill especially has the effect of controlling locust pests. The accession number of bassiana(Bals.-Criv.)Vuill TRCC16003 provided by the invention in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.7998. The strain has strong spore producing capacity, has strong insecticidal activity toward three-year-old nymphs of locusts and has LT50 (lethal time of 50%) of 4.13 days. The population quantity of the locusts can be effectively controlled by utilizing the strain. Besides, bassiana(Bals.-Criv.)Vuill has good control effects on other pests such as pine moths, ostrinia nubilalis and grubs and is worthy of popularization and application.

The invention relates to a preparation method and application of Beauveria bassiana, which especially has an effect on control of locust pests. The Beauveria bassiana (Bals. -Criv.)Vuill TRCC16001 provided by the invention has a registered number of CGMCC No.7997 in China General Microbiological Culture Collection Center. With a strong spore production ability, the strain has strong insecticidal activity on pygmy grasshoppers 3rd instar locust nymphae, and the lethal time of 50% LT50 is 3.04 days. The strain can be employed to effectively control the population quantity of pygmy grasshoppers. In addition, locust Metarhizium anisopliae also has very good prevention effect on other pests Dendrolimus punctatus, ostrinia nubilalis, grubs and the like, thus being worthy of popularization and application.

The invention relates to a dwarfing and pest-killing corn plant core-removing agent, which comprises the following raw material medicaments in percentage by weight: diethyl aminoethyl hexanoate 0.05-2.5, ethephon or paclobutrazol 0.1-3, chlorpyrifos 0.3-3, imidacloprid 0.01-0.09, pirimor 0.1-2.5 and auxiliary the balance. The medicament can complete pest prevention and control and growth regulation by one time of core-removing medicament application, wherein in one time of core-removing medicament application, various corn pests such as ostrinia nubilalis and aphid are prevented and controlled; the average corn plant height is reduced by 20 to 50 centimeters; the prevention and control rate of the ostrinia nubilalis and core-eating worms is over 80 percent; the prevention and control rateof the aphid is over 95 percent; and the corn yield is increased by more than 20 to 50 percent. The medicament is simple convenient in application, labor-saving, medicament-saving and safe, prevents harm caused by the ostrinia nubilalis and aphid in a whole period of growth of corns and completely solves the problem of pest prevention and control in a post-period of corns, thereby increasing cornyield and harvest, reducing environmental pollution and being worthy to be promoted and used.

The invention provides an insect-resistant gene Cry1Ab-Material. The synthesis of the gene is as follows: according to the amino acid sequence of the N-terminal of the original Bacillus thuringiensis (Bt) protein (Cry1Ab), the preferred codons of monocotyledon (corn) is used to perform human reformation and synthetize a new Cry1Ab DNA sequence; and the prokaryotic expression vector and plant expression vector are constructed, and transformation is performed in the host cells. Vitro tests prove that the transformed and synthetized Bt gene toxic protein has obvious insecticidal effect on Ostrinia nubilalis. The expression of the insect-resistant gene Cry1Ab-Ma in monocotyledon is stable and efficient, thus the gene can be used to produce insect-resistant transgenic plant.

The invention discloses a method for synthesizing tetradecene alcohol acetic ester in ostrinia nubilalis sex pheromone, which takes ethyltriphenylphosphonium bromide and 1, 12-codlemone as starting materials. The method comprises the following steps: the 1, 12-codlemone is subjected to a unilateral transesterification reaction to obtain 12-hydroxy dodecanol acetic ester, and then subjected to an oxidation reaction to obtain 12-oxo dodecanol acetic ester which is in a Wittig reaction with the ethyltriphenylphosphonium bromide to obtain (Z / E)-12 tetradecenoic alcohol acetic ester of high cis-trans ratio in Asian ostrinia nubilalis sex pheromone. The invention has the advantages of simple and reasonable synthesis route, convenient and safe operation, high usage ratio of raw materials, short synthesis time and the like.

The invention belongs to a synergistic insecticidal composition, a preparation method and an application thereof. The synergistic insecticidal composition is a mixture comprising a compound (A) and a compound (B). The insecticidal composition can be used for preventing and treating various pests, such as armyworms (subtropical armyworms, fall armyworms, yellowstriped armyworms and western yellowstriped armyworms), cotton bollworms, tomato pinworms, tomato fruit borer, sphinges, garden webworms, potato moths, cabbage moths, cabbage loopers, nocturnal insects, asparagus caterpillars, codling moths, peach fruitborers, oriental fruit moths, rose leaf roller stripes, codling olethreutids, plaque screen leafminer, apple leaf miners, rice stem borers, yellow rice borers, pink rice borers, rice leaf rollers, cabbage worms, ostrinia nubilalis, ostrinia furnacalis, melon worms, diaphania indica, pickleworms, oriental tobacco budworms and the like. The preventing effect is higher than that of using the compound (A) or the compound (B) singly.

The invention relates to a glycosyl substituted genipin derivative (I) as well as a preparation method and an application as pesticides. The inhibition rate of a part of compounds in formulae (Ia andIb) to tobacco mosaic viruses exceeds that of commercial variety virazole, and is approximate to that of ningnanmycin. In addition, a part of the compounds in the formulae (Ia and Ib) have excellent sterilization activity on 14 plant pathogens, and fungus bacteriosis of multiple crops can be effectively prevented and treated. A part of the compounds in the formulae (Ia and Ib) have excellent insecticide activity on 4 common lepidoptera pests (plutella xylostella, oriental armyworm, ostrinia nubilalis and cotton bollworm), and lepidoptera pests of multiple crops can be effectively prevented andtreated. Specific synthesis steps and bioactivity are shown in the specification.

The invention relates to a Bt protein in-vitro insect resistance identification method which comprises the following steps: connecting a Bt gene to a protocaryon expression vector pET-28B; transferring the protocaryon expression vector containing the Bt gene into colibacillus BL21; inducing to express the Bt protein; extracting the induction-expressed Bt protein; mixing the Bt protein into an Ostrinia nubilalis larva artificial feed, and feeding Ostrinia nubilalis larvae; and after several days, making statistics on the mortality and growth conditions of the Ostrinia nubilalis larvae. The method is simple and quick to operate, is not restricted by time and seasons, has the advantage of low economic cost, and can be used for quickly carrying out Bt protein insect resistance identification.

The invention discloses a trifluoromethylpicolinamide compound containing a chiral thioether structure and an application thereof. The general formula of the structure of the trifluoromethylpicolinamide compound is shown as I in the specification. The range of substituents in the formula is as defined in the claims. The compound (I) has good insecticidal activity and plant virus resisting activity, can be used for preventing and treating plutella xylostella, armyworm, ostrinia nubilalis and other pests, and can also be used for preventing and treating TMV and other plant virus diseases.

The invention discloses beauveria bassiana granules. The beauveria bassiana granules are prepared through a method comprising the following steps that a fermentation culture medium is inoculated with beauveria bassiana or beauveria bassiana spores, wherein the fermentation culture medium is prepared through the method that 4 g of glucose, 0.4 g of monopotassium phosphate, 0.2 g of magnesium sulfate, 0.1 g of zinc sulfate, 8 mg of vitamin B1, 1,000 g of corn stalk particles and 1.2 liters of water are added into every one liter of the PD culture medium; fermentation is conducted for 7 days in the environment in which the temperature is 25 DEG C and the absolute humidity is 100%. The beauveria bassiana granules have the effects of being capable of adapting to a low-humidity and high-temperature environment in a field, efficiently infecting ostrinia nubilalis and efficiently killing pests; especially, the beauveria bassiana granules have a very good infection effect on diapause-relieving ostrinia nubilalis larvae, and therefore an important significance on long-term pest resisting in a corn production area is achieved; meanwhile, the beauveria bassiana granules belong to a biological preparation and have the advantages of being environmentally friendly and safe.

The invention relates to pesticide mixture agent for preventing ostrinia nubilalis and the application thereof. The pesticide mixture agent is missible oil mainly prepared by abamectin, high-efficientalpha cypermethrin, emulsifier, methyl alcohol and dimethylbenzene. The emulsifier is the mixture of triphenethyl phenmethyl polyoxyethylene ether, calcium dodecylbenzene sulfonate, benzene and alcohol solvent. By weight percentage, raw materials of the mixture agent are as follows: 1.0-2.0% of abamectin, 3.0-4.0% of alpha cypermethrin, 9-16% of emulsifier, 25-40% of methyl alcohol and 40-60% ofdimethylbenzene. When in use, the pesticide mixture agent is diluted with water at 600-900-fold and sprayed in maize bell-mouth period, tasseling stage and husking period in the fields, and thus can prevent ostrinia nubilalis, effectively reduce the population quantity of the ostrinia nubilalis and obviously reduce wood-boring steams, tassel and spike buds.

The invention discloses a device for attracting and killing farmland lepidoptera pest ostrinia nubilalis larvae by using an attractant in the technical field of attracting and killing devices. The device comprises a base, a telescopic rod is fixedly installed on the upper end face of the base, an attracting and killing box is fixedly installed on the upper end face of the telescopic rod, the telescopic rod is sleeved with a sleeve, and an attracting and killing box is fixedly mounted in the middle of the outer side of the sleeve; and insect inlet hoppers are fixedly installed on the side surfaces of the attractingand killing box, a hanging rod is fixedly installed at the top end of the interior of the attracting and killing box, a trap lamp is fixedly installed on the lower end face of the hanging rod, a conical power grid is fixedly installed at the position, close to the upper end, of the interior of the attracting and killing box, the trap lamp is located in the power grid, a supporting plate is fixedly installed in the middle of the interior of the attracting and killing box, and a protective cover is arranged on the lower end face of the supporting plate. The situation that winged insects fly out upwards can be effectively prevented, common attracting and killing of reptiles and winged insects can be achieved at the same time, the insect killing efficiency is improved, and the use performance of the device is improved.

The invention discloses an application of a metarhizium anisopliae CQMa421 strain in preparation of agents for controlling ostrinia nubilalis. The metarhizium anisopliae CQMa421 strain can infect the ostrinia nubilalis, the fatality rate can be 66.5 percent; if a metarhizium anisopliae CQMa421 strain prepared by adopting a toxicity enhancing method is adopted, the fatality rate is up to 100%, LT50 is shortened by 60 percent, and the effect is very outstanding. According to the metarhizium anisopliae preparation disclosed by the invention, the various components are effectively matched with one another, a synergistic effect is achieved, and when the metarhizium anisopliae preparation is blended with water to spray, the field efficacy of ostrinia nubilalis is over 80 percent. The preparation has high ultraviolet resistance and wetting and dispersing characteristics, the biological activity of the preparation in long-term storage is maintained, and the storage time can be 12 months.

The invention relates to a method for acquiring high-activity Cry1Ai protein mutants and the mutants, and belongs to the biotechnology field. The method for acquiring the high-activity Cry1Ai protein mutants comprises the steps that continuous multi-point mutation is performed on an amino acid sequence in a loop 2 or / and a loop 3 of a Cry protein Domain II and comprises more than 10 continuous amino acid sequence changing. The insecticidal activity of the continuous multi-point mutant ModCry1Aid-loop 2 on cotton bollworm is improved by about 13 times, the activity of the continuous multi-point mutant ModCry1Aid-loop 2 on ostrinia nubilalis is improved by near 9 times compared with a Cry1Ai protein before improvement, and the activity of the continuous multi-point mutant ModCry1Aid-loop 2 and the continuous multi-point mutant ModCry1Aid-loop 3 on rice stem borer is improved by about 5-6 times compared with the Cry1Ai protein before improvement.

The invention discloses an insecticidal composition containing beauveria bassiana and spinetoram. The insecticidal composition is characterized by being prepared from an effective component A, i.e., the beauveria bassiana, and an effective component B, i.e., the spinetoram, wherein the quantity of the effective component A, i.e., the beauveria bassiana, is 5 to 40 billion spores per gram; the content of the effective component B, i.e., the spinetoram, is 5 percent to 50 percent; the preferable quantity of the effective component A, i.e., the beauveria bassiana, is 20 to 40 billion spores per gram; the preferable content of the effective component B, i.e., the spinetoram, is 10 percent to 30 percent. The insecticidal composition disclosed by the invention has a remarkable synergetic effect;the dosage of pesticide can be reduced, the residual amount of the pesticide on crops is reduced and the environment pollution is alleviated; the insecticidal composition is safe to human and livestock and has good environmental compatibility, and insect pests do not easily have drug resistance. The insecticidal composition has a function of preventing and controlling lepidoptera pests and especially has a remarkable prevention and control effect on cabbage / plutella xylostella, corn / ostrinia nubilalis and paddy rice / cnaphalocrocis medinalis.

The invention relates to an application of DMNT in preparation of a lepidoptera pest insecticide, which belongs to the technical field of pesticide preparation. DMNT is a natural secondary metaboliteof plants and has no damage to the plants. The prior art shows that DMNT has volatility and can attract natural enemies of pests to prey the pests. According to the invention, the DMNT is found to have a poisoning effect on three lepidoptera pests including plutella xylostella, ostrinia nubilalis and grassland spodoptera litura for the first time, the pupation rate and eclosion rate of the pests can be remarkably reduced, and the propagation quantity of the pests is reduced. The substance is easy to volatilize and metabolize and does not pollute soil and a water source, so that development ofa novel efficient pollution-free natural insecticide by taking the substance as a research object has important significance on prevention and control of crop pests.

The invention discloses an application of a benzamide or acylhydrazone derivative containing a 5-trifluoromethyl-4-pyrazole amide structure in the aspect of insecticidal activity. The general structure formulas are as shown in A and B in the specification, wherein R1 refers to phenyl and 3-chloropyridyl; R2-R5 refer to hydrogen atom, alkyl, hetroatom-containing alkyl, phenyl, benzyl, heterocyclyl and substituted heterocyclyl. The benzamide or acylhydrazone derivative has broad-spectrum insecticidal activity and has excellent preventing and controlling effects on diamondback moth, heliothis armigera, ostrinia nubilalis, armyworm and sanitary pests such as culex pipiens.

The invention provides application of forsythia suspensa extract in prevention and treatment of agricultural pests. The forsythia suspensa extract is an ethanol extract and can be used for preventingand treating agricultural pests such as armyworms, ostrinia nubilalis and grassland spodoptera litura and is environmentally friendly.

The invention provides Bt proteins with an insecticidal activity for ostrinia nubilalis and an application thereof. The proteins come from bacillus thuringiensis and can be at least one of Cry1Bb protein or Cry1Be protein. The several Bt proteins with a high toxicity to ostrinia nubilalis, provided by the invention, provide new gene sources for preparation of anti-ostrinia nubilalis transgenic crops and are capable of effectively delaying the generation of resistance.

The invention belongs to the technical field of biology, and discloses a biological prevention and control method for ostrinia nubilalis. The method comprises the following steps: adding a composite microbial agent into 10-20 times by weight of water, performing uniform stirring, performing uniform mixing with 20-30-mesh fine sand according to a mass ratio of 1:(50-100) to obtain pesticide sand, and spraying 2-3 g of the pesticide sand to per plant of corn for prevention and control. Compared with a conventional chemical method, the biological prevention and control method provided by the invention has the effect persistence and high control effect which can reduce pesticide application frequency, can effectively control pests, delay drug resistance of the pests, ensure safety requirementsof corn production, and protect the ecological environment; and the method is green and environmentally friendly, and has broad market application prospects.

The invention relates to ether compounds with nitrogen-containing 5-member heterocycle, represented by formula (I):The groups are as defined as specification.The compounds of the present invention have broad-spectrum insecticidal activities, and they are very effective to lepidopterous pests, including Ostrinia nubilalis, sugarcane borer, summer fruit tortrix moth, Grapholitha inopinata, Lymantria dispar, Cnaphalocrocis medialis, Pyrausta nubilalis, Heliothis assulta, Grapholitha molesta, Plutella xylostella, Laphygma exigua, Prodenialitura and the like, especially more effective to Plutella xylostella and Laphygma exigua, and can have very good effects at very low doses. And the compounds of present invention have high activities to homopteran pests such as aphid. At the same time, some compounds of present invention have very good fungicidal activities, and can be used for preventing wheat powdery mildew, cucumber downy mildew, vegetable grey mould and the like.

The present invention relates to new Zea mays plants which are resistant to both Western Corn Rootworm (WCR) and European Corn Borer (ECB) and do not express an insecticidal protein derived from Bacillus thuringiensis. The invention is also directed to hybrids, progeny and seeds of those plants.

The invention discloses a reagent for preventing and treating Ostrinia nubilalis insect damage, which comprises the following components in parts by weight: 0.5-1 part of 50% fenitrothion missible oil, 0.1-0.3 part of pyrethrin, 0.2-0.4 part of abamectin preparation, 1-3 parts of shallot juice, 5-8 parts of triperygium wilfordii and 2-4 parts of garlic oil. The reagent is simple in proportioning and convenient to use, and can effectively kill Ostrinia nubilaliss. When in use, the components are mixed and diluted with water to 1000-2000 times by mass of the mixture; and the liquor is sprayed on the spear leaf of the ginger seedling at dusk, and the Ostrinia nubilalis larvae can be killed when the liquor flows to the stem.

The invention relates to an insect-resistant fusion gene M2CryAb-VIP3A as well as an expression vector, a product and application of the insect-resistant fusion gene M2CryAb-VIP3A, and aims to solve the technical problem that the existing Bt fusion gene is difficult to give consideration to both biological safety and efficient insect killing. The nucleotide sequence of the insect-resistant fusion gene M2CryAb-VIP3A is as shown in SEQ ID NO. 4, and the corresponding protein sequence of the insect-resistant fusion gene M2CryAb-VIP3A is as shown in SEQ ID NO. 5. The fusion gene does not contain a sensitizer sequence and can be efficiently expressed, the biological safety and the insecticidal effect are both considered, and an expression product of the fusion gene has excellent resistance to pests such as ostrinia nubilalis and spodoptera frugiperda and is wider in insecticidal spectrum. After the M2CryAb-VIP3A gene is introduced into corn, a new crop variety which is resistant to stem borers and also resistant to other lepidoptera pests or coleoptera pests (such as spodoptera exigua and the like) is obtained, and the service life of the resistant variety is effectively prolonged through fusion or polymerization of the gene; the insect-resistant fusion gene expression protein M2CryAb-VIP3A disclosed by the invention has an excellent insecticidal effect, can be applied to preparation of insect-resistant preparations, and is green, environment-friendly and pollution-free.

The invention discloses a breeding method of a waxy corn variety with high resistance against diseases and pests, belonging to the technical field of corn disease-resistant breeding. In the invention, high-yield high-quality waxy corn variety, high-resistance bipolaris maydis variety, high-resistance sphacelotheca reiliana variety, high-resistance ostrinia nubilalis variety and high-resistance puccinia polysora variety are adopted as parents; and through hybridization and backcrossing polymerization and multiple generations of seed waxiness and disease resistance identification and screening, a hybrid waxy corn variety with high resistance against 4 diseases and pests, good waxiness and excellent agricultural and yield traits is obtained.

The invention discloses an insecticidal composition containing beauveria bassiana and a preparation method thereof. The insecticidal composition containing the beauveria bassiana comprises the following raw material components in percentage by mass: 10 to 50 percent of beauveria bassiana spore powder (with the content of 100 billion pores / gram), 0.01 to 5 percent of a beauveria bassiana spore germination promoting substance, 5 to 30 percent of a surfactant, 0.1 to 10 percent of a thickening agent and the balance of an oil-phase carrier. The composition disclosed by the invention is applied toprevention and control of crop insects including cnaphlocrocis medinais, ostrinia nubilalis and the like; and the composition has the advantages of good quick-acting effect, stable prevention and control effect, environment friendliness and the like.

The invention relates to an application of a compound UNC1999 in preparation of an insecticide. The structural formula of the UNC1999 is shown in the specification. The UNC1999 can be used for preparing the insecticide for preventing and treating tobacco clanis bilineata, prodenia litura, cotton bollworms, plutella xylostella, beet armyworms and ostrinia nubilalis. Compared with the prior art, theapplication field of the compound UNC1999 is widened, and the compound UNC1999 is applied to the insecticide for the first time and is used for preventing and treating various agricultural and sanitary pests and particularly has a remarkable prevention and treatment effect on tobacco clanis bilineata, prodenia litura, cotton bollworms, plutella xylostella, beet armyworms and ostrinia nubilalis.