Beauveria bassiana and application thereof

A technology of Beauveria bassiana and Beauveria bassiana, applied in the fields of application, fungi, biocides, etc.

Active Publication Date: 2014-10-08
JIANGXI TIANREN ECOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The strain was stored in the General Microorganism Center (CGMCC) of th...

Method used

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  • Beauveria bassiana and application thereof
  • Beauveria bassiana and application thereof
  • Beauveria bassiana and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Strain Identification of Beauveria bassiana

[0021] Molecular systematic identification

[0022] DNA extraction method: Quartz sand grinding CTAB extraction method (Scott et al., 2000).

[0023] Genetic markers: the translation elongation factor (tef 1-alpha) gene (tef) was selected, and the amplification primers: EF1T, EF2T (Bischoff et al., 2006).

[0024] PCR amplification reaction conditions: first heat at 94 degrees Celsius for 3 minutes to denature the template DNA, and then enter the following temperature cycles: denaturation at 94 degrees Celsius for 30 seconds, annealing at 50 degrees Celsius for 30 seconds, extension at 72 degrees Celsius for 45 seconds, a total of 35 cycles, and finally at 72 degrees Celsius Celsius extension for 5 minutes (Wang, 2012).

[0025] PCR product detection and sequencing: PCR products and 100bp DNA ladder (MBI Fermentas) were electrophoresed in 0.5×TBE electrophoresis buffer at 80V for 20 minutes on 2.0% agarose gel, and then pl...

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Abstract

The invention relates to a preparation method and application of Beauveria bassiana, which especially has an effect on control of locust pests. The Beauveria bassiana (Bals. -Criv.)Vuill TRCC16001 provided by the invention has a registered number of CGMCC No.7997 in China General Microbiological Culture Collection Center. With a strong spore production ability, the strain has strong insecticidal activity on pygmy grasshoppers 3rd instar locust nymphae, and the lethal time of 50% LT50 is 3.04 days. The strain can be employed to effectively control the population quantity of pygmy grasshoppers. In addition, locust Metarhizium anisopliae also has very good prevention effect on other pests Dendrolimus punctatus, ostrinia nubilalis, grubs and the like, thus being worthy of popularization and application.

Description

technical field [0001] Preparation method and application of a strain of Beauveria bassiana Background technique [0002] Locusts are insects of the family Acrididae, order Orthoptera. There are more than 10,000 species worldwide. Distributed in tropical and temperate grasslands and deserts all over the world. The mouthparts are hard, the front wings are narrow and tough, the hind wings are broad and soft, good at flying, and the hind limbs are well developed and good at jumping. Locusts are an important part of the agricultural, forestry, and animal husbandry ecosystems. Many harmful locust species can cause varying degrees of damage to agriculture, forestry, and animal husbandry. There are more than 10,000 species of locusts in the world, and about 300 species of locusts can cause harm to agriculture, forestry, and animal husbandry. Locusts can occur in the world except Antarctica and Eurasia north of latitude 550. [0003] Grassland locusts have been controlled by che...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
Inventor 王根豪梁小文陈晓燕周立峰李源刘丽君李肖宇马忠岩朱湘盛
Owner JIANGXI TIANREN ECOLOGY
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