1626 results about "Spore structure" patented technology

Methods are disclosed for sterilizing tissue to reduce the level of one or more active biological contaminants or pathogens therein, such as viruses, bacteria, (including inter- and intracellular bacteria, such as mycoplasmas, ureaplasmas, nanobacteria, chlamydia, rickettsias), yeasts, molds, fungi, spores, prions or similar agents responsible, alone or in combination, for TSEs and / or single or multicellular parasites. The methods involve sterilizing one or more tissues with irradiation.

A device for use with an ultrasonic transducer to lyse components of a fluid sample comprises a cartridge having a lysing chamber, an inlet port in fluid communication with the lysing chamber, and an outlet port for exit of the sample from the lysing chamber. The inlet and outlet ports are positioned to permit flow of the sample through the lysing chamber, and the chamber contains at least one solid phase for capturing the sample components to be lysed as the sample flows through the chamber. The lysing chamber is defined by at least one wall having an external surface for contacting the transducer to effect the transfer of ultrasonic energy to the chamber.

A device for lysing components (e.g., cells, spores, or microorganisms) of a fluid sample comprises a cartridge having a lysing chamber for receiving the sample and having at least one solid phase in the lysing chamber for capturing the sample components to be lysed. An ultrasonic transducer is coupled to a wall of the lysing chamber to transfer ultrasonic energy to the captured sample components.

Compositions including a non-pathogenic lactic acid-producing bacteria, such as a Bacillus species, spores or an extracellular product of B. coagulans, formulated for oral administration to the intestinal tract for inhibiting bacterial gastrointestinal infections are described. Methods and systems using the compositions for treating gastrointestinal infections, particularly sudden infant death syndrome (SIDS) are also disclosed.

A method and apparatus is described for neutralizing airborne pathogens and chemical toxins in ventilated air, and in heating or air conditioning systems. The pathogen-chemical toxin neutralization system is effective against a wide spectrum of pathogens and toxins, it incorporates commercially available components, and it can be readily integrated into commercial HVAC systems where it decontaminates large volumes of ventilated air in real time without any chemical reagents. The system has a flow-through reaction chamber (101) that contains a UV light source (106) that emits short intense flashes of broad-spectrum UV light, a source aqueous hydrogen peroxide that can be a reservoir or a hydrogen peroxide generator (106), and optionally a source of ozone. The interaction of UV light and hydrogen peroxide generates hydroxyl radicals that neutralize pathogens and chemical toxins as they pass through the reaction chamber (101) in real time. The pathogens that can be neutralized by this system include bacteria, viruses, spores, fungi and parasites.

The present invention discloses compositions derived from an isolated Bacillus species, spores, or an extracellular product of Bacillus coagulans comprising a supernatant or filtrate of a culture of said Bacillus coagulans strain, suitable for topical application to the skin or mucosal membranes of a mammal, which are utilized to inhibit the growth of bacterium, yeast, fungi, virus, and combinations thereof. The present invention also discloses methods of treatment and therapeutic systems for inhibiting the growth of bacterium, yeast, fungi, virus, and combinations thereof, by topical application of therapeutic compositions which are comprised, in part, of isolated Bacillus species, spores, or an extracellular product of Bacillus coagulans comprising a supernatant or filtrate of a culture of said Bacillus coagulans strain. In addition, the present invention also discloses compositions, methods of treatment, and therapeutic systems for inhibiting the growth of bacterium, yeast, fungi, virus, and combinations thereof, comprising an extracellular product of Pseudomonas lindbergii comprising a supernatant or filtrate of a culture of said Pseudomonas lindbergii strain.

This invention relates to a method, process and apparatus for disinfecting and sterilizing all types of surfaces contaminated with microorganisms and toxic substances to render both inactive. Furthermore, this invention relates to both a method and apparatus for disinfecting and / or sterilizing breathable air and then using this air to protect a confined space from external contamination. The apparatus consists of a new ultra-violet (NUV) source that is more effective than mercury based 254 nm light for destroying DNA of virus, bacteria, spores and cists. It is most effective in breaking chemical bonds in toxic gases and Biotoxins that are useful to terrorists. It is combined with other apparatus that remove particulates and byproducts sometimes produced by the NUV source and maintains positive pressure of the confined space so as to prevent the influx of air from outside the protected zone.

The subject invention provides novel and efficacious systems and methods for particle, chemical, and / or biocompound sensing. In one embodiment, the system of the invention comprises a sensing device that includes a membrane containing at least one nanochannel that spans all or substantially all of the thickness of the membrane. The nanochannel(s) of the invention can be functionalized to enhance target analyte detection and quantification. In one embodiment, the nanochannel is conically shaped and includes a molecular recognition agent for a target analyte. In certain operations, the sensing systems of the invention quantitatively and qualitatively detect biochemical / biomedical species and biomacromolecules, such as proteins, DNA, cells, spores and viruses, with a high degree of sensitivity and specificity.

A pesticidally active isolate of the fungus Beauveria bassiana, which is monosporic and which may be used in agriculture as a pesticide for the effective control of pests. The biomass of the inventive isolate contains an unusually high proportion of spores. The invention also provides methods for the preparation of the isolate and methods of formulating and using same for the effective control of crop infesting pests.

Current chemotherapeutic approaches for cancer are in part limited by the inability of drugs to destroy neoplastic cells within poorly vascularized compartments of tumors. We have here systematically assessed anaerobic bacteria for their capacity to grow expansively within avascutar compartments of transplanted tumors. Among 26 different strains tested, one (Clostridium novyi) appeared particularly promising. We created a strain of C. novyi devoid of its lethal toxin (C. novyi-NT) and showed that intravenously injected C. novyi-NT spores germinated within the avascular regions of tumors in mice and destroyed surrounding viable tumor cells. When C. novyi-NT spores were administered together with conventional chemotherapeutic drugs, extensive hemorrhagic necrosis of tumors often developed within 24 hours, resulting in significant and prolonged anti-tumor effects. This strategy, called combination bacteriolytic therapy (COBALT), has the potential to add a valuablle dimension to the treatment of cancer.

A biosensor system is provided for detecting biological species such as bacteria, proteins, viruses, spores and DNA or RNA. The biosensor system may also be able to distinguish between Gram positive and Gram negative bacteria. In some embodiments, the analyte solution flows past a detector surface to a filter, which traps the analyte. The analyte is then washed back past the detector surface, thus increasing the number of analyte species that become attached to the surface. The detector may include an optical microresonator that is optically coupled via waveguides to a light source and an optical detector. One of the waveguides may be provided with a wavelength selective reflector to increase the amount of probe light coupled into the microresonator or to increase the fraction of the signal light detected by the optical detector.

The present invention provides a composition, comprising: greater than about 0.1% by weight hydrogen peroxide; an aromatic acid component; surfactant; optionally, a solvent; and a carrier. The composition of the invention is useful as a disinfecting composition for killing microorganisms such as bacterium (including Mycobacterium), spores and fungi. The composition provides a pathogenic bacteria kill rate of 99.9% in about 30 seconds when bacteria are exposed to the composition and is effective in providing a Mycobacterium kill of 106 with two minutes or less. Moreover, the compositions of the invention are generally more resistant to catalase deactivation than, for example, an aqueous solution of hydrogen peroxide. The concentration of hydrogen peroxide within the composition may range from about 1% by weight to about 7% by weight and the concentration of aromatic acid component may range from about 0.1% by weight to about 5% by weight. The invention also provides a method for disinfection of a substrate utilizing the composition. The composition of the invention may be used in the foregoing method on a medical instrument, such as an endoscope or the like. Applying the compositions to a substrate may be accomplished in any of a variety of application methods such as by roll coating, dipping, spraying, or rotational tumbling. The composition may be applied to the substrate for a period of time ranging from about 30 seconds to about ten minutes. In this aspect, the invention can further comprise drying the substrate after removing the composition.

A swab comprising gelatine or collagen has been found to have a remarkably high recovery of microorganisms. Furthermore, the samples, such as microorganisms, spores, nucleotides and other biologically or biochemically relevant compounds can be fully recovered from the collagen- or gelatine-comprising swab. The invention thus provides a method and swab which has a high recovery of a target from a sample and furthermore a second high recovery when transferring from the swab to a medium for analysis.

Germicidal compositions with enhanced activity towards killing microbiological spores and vetgetative cells comprising certain quaternary ammonium compounds (QACs), phenolic compounds, monohydric alcohols, hydrogen peroxide, iodine, triclocarban, triclosan or combinations thereof with one or more spore coat opening agents. The invention also provides for the application of the germicidal compositions to animate and inanimate surfaces to help kill germs and protect against the risk of infection from bacteria, molds, yeasts, fungi, viruses, and microbiological spores.

The invention discloses a composite type artificial algal reef comprising a mature algal reef and a seedling reef. The mature algal reef comprises a solid base. The upper surface of the solid base is embedded with a metal frame, wherein the shape of the metal frame is identical to that of the upper surface of the solid base and the size of the metal frame is identical to that of the upper surface of the solid base. A conch net bag is arranged in the metal frame. The center of the upper surface is embedded with a metal stand column. A steel ring is welded to the upper end of the stand column. A plurality of seedling ropes are fixed between the metal frame and the steel ring. The seedling reef is fixed to the side face of the solid base of the mature algal reef, and algal seedlings are attached to the seedling reef. By the adoption of the composite type artificial algal reef, the attaching effect of alga is guaranteed, the repair period of an algae field is shortened, the success rate of repair and reconstruction of the algae field is improved, the adhesive rate of the seedlings is increased, and the seedling reef is firm, durable, small in size, light, and convenient to carry; the net bag made of waste conches is placed in the metal frame, and a biophile reef can be composed through diffusion and attachment of spores of fronds on the seedling ropes; resource utilization of the waste conches is achieved, and composite type artificial algal reef has the advantages of being economical and easy and convenient to use.

Compositions, methods and business applications of using new and recycled cardboard infused with a plurality of saprophytic (including endophytic) and mycorrhizal fungi matched with seeds of plants (including trees, vegetables, herbs and grasses) whereby the cardboard can be sprouted by end-users to start ecosystems. Such containers may have carbon-credit value for companies and consumers when planted and grown as a carbon sink or carbon offset for the photosynthetic and mycelial sequestration of carbon dioxide. The relative weight of the Life Box's added seeds and spores does not significantly affect the total weight of the infused cardboard, thus not increasing transportation costs.

The invention relates to enterococcus faecium for feeding as well as a freeze-drying fungicide and applications thereof, and discloses enterococcus faecium LAB12 CGMCC (China General Microbiological Culture Collection Center) No.4847 which is grampositive cocci, has no spores, grows well on an MRS agar plate, forms a round bacterial colony with the diameter of 0.5-1mm within 48 hours and is used for feeding, and the bacterial colony is round, smooth and upheaved, and is a shape of grey white dewdrop; the enterococcus faecium grows in a facultative anaerobic condition; the growth temperature range is 10 DEG C-45 DEG C; the optimum growth temperature is 30 DEG C-40 DEG C; and the growth pH value is 4-10, and the optimum pH value is 6.0. The freeze-drying fungicide formed by the bacterial strain is nontoxic and harmless, is gastric juice resistant, is cholate resistant, has a high inhibitory effect for multiple harmful bacteria, has a long quality guarantee period, can be widely applied to birds and livestock breeding to strengthen the animal disease-resistant capability, and is expected to be the substitution of antibiotics for feeding.

Compositions including an isolated Bacillus species, spores or an extracellular product of B. coagulans, suitable for topical application, for inhibiting growth of yeast, fungus, bacteria or Herpes simplex virus are disclosed. Methods of inhibiting growth of yeast, fungus, bacteria or Herpes simplex virus by topical application of compositions that include an isolated Bacillus species, spores or an extracellular product of a B. coagulans strain are disclosed.

The present invention relates to a stable antimicrobial and cleaning compositions including an amine antimicrobial agent; a borate salt; and spores (bacterial or fungal), vegetative bacteria, fungi, or enzyme, and to methods of using the composition. The composition can also include a polyol.

Compositions including an isolated Bacillus species, spores or an extracellular product of B. coagulans, suitable for topical application, for inhibiting growth of yeast, fungus, bacteria or Herpes simplex virus are disclosed. Methods of inhibiting growth of yeast, fungus, bacteria or Herpes simplex virus by topical application of compositions that include an isolated Bacillus species, spores or an extracellular product of a B. coagulans strain are disclosed.

The present invention discloses a composition and method of providing ionic forms or compounds of any combination of three metals to produce a product that can be used as a antimicrobial as defined by the U.S. Environmental Protection Agency (EPA) such as a hard surface disinfectant and as a foliar spray or water treatment for the control of various diseases in garden row, field and tree crops, on hard surface areas such as equipment from infected fields or in hospitals, homes, etc. as well as against a wide range of human, plant and animal diseases. Additionally, the composition of the present invention is able to be used to coat and otherwise treat and disinfect food products such as but not limited to fish and shellfish, meat, milk, poultry, eggs and irrigated crops (both food and nonfood crops) as well as non food products as defined in the EPA exemption from tolerance for copper and copper products. The composition may include inerts such as surfactants, detergents and buffers to adjust the pH. The composition remains soluble in water and is useful efficacious against the spread of bacteria, viruses and fungi and other pathogens within a source of water such as a reservoir or pool. Alternatively, the composition may be in aerosolized, misted, vaporized, fogged, humidified forms to produce micronized particles which are able to remain in suspension in the air for long periods of time in order to act on air-borne fungal spores and / or pathogens.

The present invention utilizes fungal spore mass or hyphal fragments in burlap bags or sacks filled with biodegradable materials. The fungi may include saprophytic fungi, including gourmet and medicinal mushrooms, mycorrhizal fungi, entomopathogenic fungi, parasitic fungi and fungi imperfecti. The fungi function as keystone species, delivering benefits to both the microsphere and biosphere. Such fungal delivery systems are useful for purposes including ecological rehabilitation and restoration, preservation and improvement of habitats, bioremediation of toxic wastes and polluted sites, filtration of agricultural, mine and urban runoff, improvement of agricultural yields and control of biological organisms.

Compositions including an isolated Bacillus species, spores or an extracellular product of B. coagulans, suitable for topical application, for inhibiting growth of yeast, fungus, bacteria or Herpes simplex virus are disclosed. Methods of inhibiting growth of yeast, fungus, bacteria or Herpes simplex virus by topical application of compositions that include an isolated Bacillus species, spores or an extracellular product of a B. coagulans strain are disclosed.

The present invention relates to a stable solid cleaning composition including a borate salt and spores (bacterial or fungal), vegetative bacteria, or fungi, and to methods of using the composition.

The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins, formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth and for protecting plants from pathogens by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plant roots.

The invention relates to a biological agent against crucifer club root and application thereof, belonging to the technical field of bio-pesticide. The strain for production is Bacillus subtilis XF-1, whose preserving number is CGMCC NO.2357. The stain has the characteristics as follows: (1) the primary colony on the LB culture substrate is white and round having a wet surface; the later colony is light yellow having uneven edge with dry and crimple surface; observed from the microscope, the strain is short-bar shaped and movable with spore, peritricha and dimension of 0.7 - 0.8 * 2.0-2.4 mum; (2) the strain is Gram-positive and aerobic and makes use of glycogen, sugar, citrate, gelatin hydrolysate, starch and casein, but does not make use of cellulose, tyrosine and catalase positive; (3) the stain has the function of sterilization, disease prevention, and yield improvement. The embodiment of the invention is as follows: using the test tube of Bacillus subtilis XF-1 stain, shake cultivation, and culture solution for fermentation to prepare biological agent, then applying the biological agent to the rhizosphere soil of crucifer crops, thereby having good effect in preventing and treating, and simple production.

The present invention relates to electrochemically treated water, obtainable by means of a method that is characterized by the following steps: a) electrolyzing water and b) reducing the concentrations of the oxidants created in step a). The electrochemically treated water obtained in this manner is characterized by a disinfecting effect on bacteria, bacterial spores, fungi, fungal spores, viruses, prions, or mixtures thereof. The invention further relates to water, characterized in that the same has a disinfecting effect on bacteria, bacterial spores, fungi, fungal spores, viruses, algae, prions, or mixtures thereof, that it is substantially free of disinfection agents with the exception of the oxidants related to the disinfection agents, and that it has a total concentration of oxidants of less than 20 ppm.

The present invention discloses a preparation method and application of Bacillus coagulans and a spore preparation cultured in high density, and belongs to the microbe technical field. A Bacillus coagulans strain is classified and named Bacillus coagulans JSSW-LA-07 and is preserved as CGMCC No.2602 in China General Microbiological Culture Collection Center. The Bacillus coagulans strain JSSW-LA-07 can produce spore under an aerobic condition and produce a small amount of L-lactic acid in the metabolism during a course of fermentation. The Bacillus coagulans strain can produce L-lactic acid with a higher purity under an anaerobic condition. The strain does not produce substances which can be harmful to human and cultured animals, has the characteristics of innocuity, on residue, no side effect and the like, has a great significance for production of green and safe livestock, fowls and marine products and improvement of cultivation ecological environment, and can be widely applied in the cultivation production of high yield, high quality, high efficiency, synusiologic and safe livestock, fowls and marine products, and the regulation and control of the ecological environment of cultivation.

This invention relates to a method of sterilizing air that contains airborne pathogenic agents or microorganisms such as spores, bacteria, viruses, yeasts and moulds The method consists in damaging and braking up the contaminant molecular structure by oxidation and ionization and chemical bond rupture. Through this process the microorganisms or the contaminants are inactivated through structural damages to the cellular membrane and / or the nucleus membrane and / or to the DNA structure. This is accomplished by creating through the air to be treated, various electronic current having a plurality of specific controlled average kinetic energies matching the desired chemical bonds to be affected and / or ruptured. The air interaction with such various currents, having specific energies, sufficient intensities and exposition time is accomplished through several stages to completely sterilize the air or gas. The air or gas is then processed to remove undesirable by-products that may be present so that it can be used directly or stored.