35 results about "Exosporium" patented technology

Herein a Bacillus exosporium antigen delivery (BEAD) system that provides a means to introduce recombinant proteins or small molecules into the exosporium of members of the B. cereus family of bacteria, i.e. B. anthracis, B. cereus, and B. thuringiensis, is disclosed. The system results in the surface display of recombinant proteins or small molecules such that they can stimulate an immune response. In addition, methods of making and using the system are described.

The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins, formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth and for protecting plants from pathogens by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plant roots.

The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins and formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth, for protecting plants from pathogens, and for enhancing stress resistance in a plant by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plants.

The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins, formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth and for protecting plants from pathogens by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plant roots.

Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifiations of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

Herein a Bacillus exosporium antigen delivery (BEAD) system that provides a means to introduce recombinant proteins or small molecules into the exosporium of members of the B. cereus family of bacteria, i.e. B. anthracis, B. cereus, and B. thuringiensis, is disclosed. The system results in the surface display of recombinant proteins or small molecules such that they can stimulate an immune response. In addition, methods of making and using the system are described.

The present invention relates to a method for expressing a target protein on an exosporium forming the outermost surface of bacterial spores. More particularly, the present invention relates to a method for expressing a target protein on the surface of cells and spores using an exosporium as a matrix for surface expression, and methods for the production of a protein array, the production of antibodies, the separation of a certain substance from a mixture, bioconversion, and the improvement of a target protein, which are characterized by using the cells or spores having the target protein that was expressed on the surface by the above expression method. The method for expressing the target protein on the surface of the spore outer membrane of the gene carriers according to the present invention has effects in that a variety of the target proteins can be expressed and the level of surface expression of the target protein is increased compared to the existing technology, and also the structural stability of the gene carriers having the target protein expressed on their surface, the viability of the host, and the rapidity of the screening method, are greatly increased.

The present invention relates to a composition comprising a) recombinant exosporium-producing Bacillus cells that express a fusion protein comprising: (i) at least one plant growth stimulating protein or peptide and (ii) a targeting sequence that localizes the fusion protein to the exosporium of the Bacillus cells; and b) at least one further biological control agent selected from particular microorganisms disclosed herein and / or a mutant of a specific strain of a microorganism disclosed herein having all identifying characteristics of the respective strain, and / or at least one metabolite produced by the respective strain, and / or at least one metabolite produced by the respective strain that exhibits activity against insects, mites, nematodes and / or phytopathogens in a synergistically effective amount. Furthermore, the present invention relates to the use of this composition as well as a method for enhancing plant growth, promoting plant health, and / or reducing overall damage of plants and plant parts.

Fusion proteins, recombinant Bacillus cereus family members that express fusion proteins, and exosporium fragments derived from spores of the recombinant Bacillus cereus family members are provided. Compositions comprising the spores or exosporium fragments are also provided. Methods involving the use of spores of recombinant Bacillus cereus family members and exosporium fragments derived from spores of a recombinant Bacillus cereus family member in the fields of animal health and aquaculture are provided. In particular, methods are for provided for using such spores or exosporium fragments for protecting an animal or an aquatic organism from a pathogen. Methods are also provided for using exosporium fragments for producing an immunogenic response in an aquatic animal. Products for use in protecting animals from pathogens are also provided, including adhesive patches, wound dressings, insert trays for livestock footbaths, hoof bandages, feed, feed additives, and insect foggers.

The present invention relates to a composition comprising a) recombinant exosporium-producing Bacillus cells that express a fusion protein comprising: (i) at least one plant growth stimulating protein or peptide and (ii) a targeting sequence that localizes the fusion protein to the exosporium of the Bacillus cells; and b) at least one further biological control agent selected from particular microorganisms disclosed herein and / or a mutant of a specific strain of a microorganism disclosed herein having all identifying characteristics of the respective strain, and / or at least one metabolite produced by the respective strain, and / or at least one metabolite produced by the respective strain that exhibits activity against insects, mites, nematodes and / or phytopathogens in a synergistically effective amount. Furthermore, the present invention relates to the use of this composition as well as a method for enhancing plant growth, promoting plant health, and / or reducing overall damage of plants and plant parts.

The present invention relates to a method for expressing a target protein on the surface of a microorganism using Bacillus anthracis exosporium protein. More particularly, to an expression vector constructed such that it comprises bclA gene encoding Bacillus anthracis exosporium protein BclA or fragments thereof as a cell surface anchoring motif and the target protein can be expressed on the surface of a cell in a form fused with BclA or a fragment thereof when the gene encoding the target protein is expressed in a host cell, as well as, a method for expressing a target protein on the surface of a microorganism using the vector. The expression vector according to the present invention is capable of effectively expressing a target protein or a peptide on the cell surface using BclA, Bacillus anthracis exosporium protein as a cell surface anchoring motif, and since a target protein can be stably expressed on the cell surface in large amounts by culturing a microorganism transformed with the expression vector, thus making it possible to effectively use for the various purposes of recombinant live vaccines, whole cells absorbents, whole cell bioconversion and the like.

Fusion proteins, recombinant Bacillus cereus family members that express fusion proteins, and exosporium fragments derived from spores of the recombinant Bacillus cereus family members are provided. Compositions comprising the spores or exosporium fragments are also provided. Methods involving the use of spores of recombinant Bacillus cereus family members and exosporium fragments derived from spores of a recombinant Bacillus cereus family member in the fields of animal health and aquaculture are provided. In particular, methods are for provided for using such spores or exosporium fragments for protecting an animal or an aquatic organism from a pathogen. Methods are also provided for using exosporium fragments for producing an immunogenic response in an aquatic animal. Products for use inprotecting animals from pathogens are also provided, including adhesive patches, wound dressings, insert trays for livestock footbaths, hoof bandages, feed, feed additives, and insect foggers.

The present invention relates to a composition comprising a) recombinant exosporium-producing Bacillus cells that express a fusion protein comprising: (i) at least one plant growth stimulating protein or peptide and (ii) a targeting sequence that localizes the fusion protein to the exosporium of the Bacillus cells; and b) at least one particular insecticide disclosed herein in a synergistically effective amount. Furthermore, the present invention relates to the use of this composition as well as a method for enhancing plant growth, promoting plant health, and / or reducing overall damage of plants and plant parts.

The present invention relates to a composition comprising a) recombinant exosporium-producing Bacillus cells that express a fusion protein comprising: (i) at least one plant growth stimulating protein or peptide and (ii) a targeting sequence that localizes the fusion protein to the exosporium of the Bacillus cells; and b) at least one particular fungicide disclosed herein in a synergistically effective amount. Furthermore, the present invention relates to the use of this composition as well as a method for enhancing plant growth, promoting plant health, and / or reducing overall damage of plants and plant parts.

The present invention relates to a composition comprising a) recombinant exosporium-producing Baciillus cells that express a fusion protein comprising: (i) at least one plant growth stimulating protein or peptide and (ii) a targeting sequence that localizes the fusion protein to the exosporium of the Baciillus cells; and b) at least one particular fungicide disclosed herein in a synergistically effective amount. Furthermore, the present invention relates to the use of this composition as well as a method for enhancing plant growth, promoting plant health, and / or reducing overall damage of plants and plant parts.

The invention belongs to the field of the preparation of a biological material, and relates to a preparation method and an application of a bacterial spore functional microsphere. By virtue of a novel biochemical method, bacterial spores are processed so as to prepare a functional microsphere which is high in dispersity, and a nano material is immobilized on the surfaces of the processed pores; the functional microsphere can be applied to various fields. The preparation method is characterized by comprising the following steps: collecting bacterial spores which are cultivated for 3-10 days and are at 108-1011cfu in concentration; carrying out centrifugal washing and ultrasonic treatment; processing the bacterial pores with a trypsin liquid and a spore coating removal processing fluid, so as to finally obtain a bacterial spore microsphere that spore exosporium and spore coating are removed and surface is smooth; the microsphere is used for loading metal nanoparticles. The functional microsphere prepared by the invention is more stable in mechanical rigid structure, the loaded nano material is not easy to peel off, and the functional microsphere, as a carrier, is more stable and reliable in performance. The invention also discloses an application of the prepared spore microsphere in an animal immunization detection material.

The present invention relates to a method for expressing a target protein on an exosporium forming the outermost surface of bacterial spores. More particularly, the present invention relates to a method for expressing a target protein on the surface of cells and spores using an exosporium as a matrix for surface expression, and methods for the production of a protein array, the production of antibodies, the separation of a certain substance from a mixture, bioconversion, and the improvement of a target protein, which are characterized by using the cells or spores having the target protein that was expressed on the surface by the above expression method. The method for expressing the target protein on the surface of the spore outer membrane of the gene carriers according to the present invention has effects in that a variety of the target proteins can be expressed and the level of surface expression of the target protein is increased compared to the existing technology, and also the structural stability of the gene carriers having the target protein expressed on their surface, the viability of the host, and the rapidity of the screening method, are greatly increased.

The present invention relates to a composition comprising a) recombinant exosporium-producing Bacillus cells that express a fusion protein comprising: (i) at least one plant growth stimulating protein or peptide and (ii) a targeting sequence that localizes the fusion protein to the exosporium of the Bacillus cells; and b) at least one further biological control agent selected from particular microorganisms disclosed herein and / or a mutant of a specific strain of a microorganism disclosed herein having all identifying characteristics of the respective strain, and / or at least one metabolite produced by the respective strain, and / or at least one metabolite produced by the respective strain that exhibits activity against insects, mites, nematodes and / or phytopathogens in a synergistically effective amount. Furthermore, the present invention relates to the use of this composition as well as a method for enhancing plant growth, promoting plant health, and / or reducing overall damage of plants and plant parts.

The present invention relates to a composition comprising a) recombinant exosporium-producing Bacillus cells that express a fusion protein comprising: (i) at least one plant growth stimulating protein or peptide and (ii) a targeting sequence that localizes the fusion protein to the exosporium of the Bacillus cells; and b) at least one particular insecticide disclosed herein in a synergistically effective amount. Furthermore, the present invention relates to the use of this composition as well as a method for enhancing plant growth, promoting plant health, and / or reducing overall damage of plants and plant parts.

A process for extracting the spore oil from the wall-broken spore of glossy ganoderma includes such steps as proportionally mixing said wall-broken spore powder with sesban powder and water, stirring, squeezing for shaping, drying, supercritical CO2 and edible alcohol extracting, vacuum separating to release CO2 gas, and vacuum drying.

The present invention relates to a composition comprising a) recombinant exosporium-producing Bacillus cells that express a fusion protein comprising: (i) at least one plant growth stimulating protein or peptide and (ii) a targeting sequence that localizes the fusion protein to the exosporium of the Bacillus cells; and b) tioxazafen in a synergistically effective amount. Furthermore, the present invention relates to the use of this composition as well as a method for enhancing plant growth, promoting plant health, and / or reducing overall damage of plants and plant parts.

The present invention relates to a composition comprising a) recombinant exosporium-producing Bacillus cells that express a fusion protein comprising: (i) at least one plant growth stimulating protein or peptide and (ii) a targeting sequence that localizes the fusion protein to the exosporium of the Bacillus cells; and b) at least one further biological control agent selected from particular microorganisms disclosed herein and / or a mutant of a specific strain of a microorganism disclosed herein having all identifying characteristics of the respective strain, and / or at least one metabolite produced by the respective strain, and / or at least one metabolite produced by the respective strain that exhibits activity against insects, mites, nematodes and / or phytopathogens in a synergistically effective amount. Furthermore, the present invention relates to the use of this composition as well as a method for enhancing plant growth, promoting plant health, and / or reducing overall damage of plants and plant parts.

The present invention relates to a method for expressing a target protein on the surface of a microorganism using Bacillus anthracis exosporium protein. More particularly, to an expression vector constructed such that it comprises bclA gene encoding Bacillus anthracis exosporium protein BclA or fragments thereof as a cell surface anchoring motif and the target protein can be expressed on the surface of a cell in a form fused with BclA or a fragment thereof when the gene encoding the target protein is expressed in a host cell, as well as, a method for expressing a target protein on the surface of a microorganism using the vector. The expression vector according to the present invention is capable of effectively expressing a target protein or a peptide on the cell surface using BclA, Bacillus anthracis exosporium protein as a cell surface anchoring motif, and since a target protein can be stably expressed on the cell surface in large amounts by culturing a microorganism transformed with the expression vector, thus making it possible to effectively use for the various purposes of recombinant live vaccines, whole cells absorbents, whole cell bioconversion and the like.

The present invention relates to a composition comprising a) recombinant exosporium-producing Bacillus cells that express a fusion protein comprising: (i) at least one plant growth stimulating protein or peptide and (ii) a targeting sequence that localizes the fusion protein to the exosporium of the Bacillus cells; and b) at least one particular insecticide disclosed herein in a synergistically effective amount. Furthermore, the present invention relates to the use of this composition as well as a method for enhancing plant growth, promoting plant health, and / or reducing overall damage of plants and plant parts.

Signal sequences useful for targeting proteins and peptides to the surface of endospores produced by Brevibacillus, Lysinibacillus, or Viridibacillus family members and methods of using the same are provided. The display of heterologous molecules, such as peptides, polypeptides and other recombinant constructs, on the exosporium of Brevibacillus, Lysinibacillus, or Viridibacillus family members, using particular N-terminal targeting sequences and derivatives of the same, and likewise are provided.

The invention belongs to the field of the preparation of a biological material, and relates to a preparation method and an application of a bacterial spore functional microsphere. By virtue of a novel biochemical method, bacterial spores are processed so as to prepare a functional microsphere which is high in dispersity, and a nano material is immobilized on the surfaces of the processed pores; the functional microsphere can be applied to various fields. The preparation method is characterized by comprising the following steps: collecting bacterial spores which are cultivated for 3-10 days and are at 108-1011cfu in concentration; carrying out centrifugal washing and ultrasonic treatment; processing the bacterial pores with a trypsin liquid and a spore coating removal processing fluid, so as to finally obtain a bacterial spore microsphere that spore exosporium and spore coating are removed and surface is smooth; the microsphere is used for loading metal nanoparticles. The functional microsphere prepared by the invention is more stable in mechanical rigid structure, the loaded nano material is not easy to peel off, and the functional microsphere, as a carrier, is more stable and reliable in performance. The invention also discloses an application of the prepared spore microsphere in an animal immunization detection material.

Signal sequences useful for targeting proteins and peptides to the surface of endospores produced by Brevibacillus, Lysinibacillus, or Viridibacillus family members and methods of using the same are provided. The display of heterologous molecules, such as peptides, polypeptides and other recombinant constructs, on the exosporium of Brevibacillus, Lysinibacillus, or Viridibacillus family members, using particular N-terminal targeting sequences and derivatives of the same, and likewise are provided.

The present invention relates to a fusion protein having a targeting sequence, exosporium protein, or exosporium protein fragment that targets the fusion protein to the exosporium of a recombinant Bacillus cereus family member and a pectinase enzyme, wherein the pectinase is a pectate lyase from Bacillus spp. having any of SEQ ID NOs: 213-217 and 222-226 or a polygalaturonase from Aspergillus niger or certain Bacillus species that can have any of SEQ ID NOs: 210-212, 218-221, and 227. The present invention also provides a recombinant Bacillus cereus family member that expresses such fusion protein and exosporium fragments derived from such recombinant Bacillus cereus family member. Methods of using such recombinant Bacillus cereus family members or exosporium fragments derived therefrom for plant growth promotion are also provided.