194 results about "Bacterial spore" patented technology

Methods and apparatus for implementing microfluidic analysis devices are provided. A monolithic elastomer membrane associated with an integrated pneumatic manifold allows the placement and actuation of dense arrays of a variety of fluid control structures, such as structures for isolating, routing, merging, splitting, and storing volumes of fluid. The fluid control structures can be used to implement a pathogen detection and analysis system including integrated immunoaffinity capture and analysis, such as polymerase chain reaction (PCR) and capillary electrophoresis (CE) analysis. An analyte solution can be input into the device and pumped through a series of immunoaffinity capture matrices in microfabricated chambers having antibodies targeted to the various classes of microbiological organisms such as bacteria, viruses and bacterial spores. The immunoaffinity chambers can capture, purify, and concentrate the target for further analysis steps.

The present invention is directed to a method for the disinfection and sterilization of material and surfaces contaminated with one or more members selected from the group consisting of bacteria and bacterial spores, comprising the steps of: (a) providing a biocidal fluid containing a mixture of effective amounts of a germinant and a germicide; and (b) contacting the material and surfaces contaminated with one or more members selected from the group consisting of bacteria and bacterial spores, with the biocidal fluid of step (a) for a time sufficient for disinfecting and sterilizing said material. The invention also provides a sterilizing composition suitable for killing and rendering spores lifeless comprising: (a) an effective amount of a germinating agent; (b) an effective amount of a germicide.

Apparatuses and methods for analyzing particles using an impedance sensor. A flow-through impedance sensor may use two in-line electrodes driven in counter phase. A common sensor electrode may be used to, for example, detect impedance and determine trajectories through the sensor area. The sensor may be used in a wide variety of applications, including but not limited to use with microfluidic devices for determining particle characteristics such as position, velocity, size, and concentration as well as detection of bacterial spores and other biological agents of potential use in warfare and bio-terrorism.

Antibacterial compositions effective against both gram positive and gram negative vegetative bacteria plus harmful gram positive sporeforming bacteria include propionibacterial metabolites in combination with two or more of the following: a lantibiotic; a lytic enzyme; and an organic acid or its salt. Methods of use are provided, as well as food products treated with these antibacterial compositions.

The present invention relates generally to chemical and biological decontamination solutions and methods of using them. The invention is useful for decontaminating a wide range of compounds and organisms. In particular, the systems, methods, solutions, and formulations of the invention can be used to remove and/or neutralize organophosphates and other toxic chemicals, bacteria, bacterial spores, fungi, molds and viruses.

In one aspect, the present invention is directed to a plant or plant part coated with a composition comprising a bacterial spore and a germinative compound. In another aspect the present invention is directed to a method of enhancing the growth of a plant or plant part comprising coating such plant or plant part with such a composition. In yet another aspect, the present invention is directed to a composition comprising a bacterial spore and a germinative compound where such components are maintained in an inactive form. The present invention also relates to use of the compositions in wastewater treatment, environmental remediation, oil recovery, aquaculture systems, and direct fed microbials.

Sterilization methods and apparatus are effective to achieve a 6-log reduction in CFUs of industry standard bacteria and bacterial spores, i.e., B. stearothermophilus and B. subtilis spores, by subjecting sterilizable materials to a chemical additive-containing carbon dioxide sterilant fluid at or near its supercritical pressure and temperature conditions. Most preferably, the chemical additive-containing supercritical carbon dioxide sterilant fluid is agitated during sterilization, e.g., via mechanical agitation or via pressure cycling.

Aerosols that include ceragenin compounds (i.e., ceragenin-containing aerosols), methods for delivering such aerosols, and devices for delivering such aerosols. Ceragenins can be used to kill a broad range of microbes (e.g., bacteria, viruses, fungi, bacterial spores, fungal spores, and the like), yet they are thought to be completely non-toxic to humans and other higher eukaryotes. Aerosols can penetrate into the nooks and crannies of a space and kill microbes that may lurk in a space. In addition, ceragenins have a extended mode of action, which allows them to continue killing microbes in a space for an extended period of time.

A method and an apparatus for detecting and quantifying bacterial spores on a surface. In accordance with the method: a matrix including lanthanide ions is provided on the surface containing the bacterial spores; functionalized aromatic molecules are released from the bacterial spores on the surface; a complex of the lanthanide ion and the aromatic molecule is formed on the surface; the complex of the lanthanide ion and the aromatic molecule is excited to generate a characteristic luminescence of the complex on the surface; and the bacterial spores exhibiting the luminescence of the complex on the surface are detected and quantified.

This invention relates to a method, process and apparatus for disinfecting and sterilizing all types of surfaces contaminated with microorganisms and toxic substances to render both inactive. Furthermore, this invention relates to both a method and apparatus for disinfecting and/or sterilizing breathable air and then using this air to protect a confined space from external contamination. The apparatus consists of a new ultra-violet (NUV) source that is more effective than mercury based 254 nm light for destroying DNA of virus, bacteria, spores and cists. It is most effective in breaking chemical bonds in toxic gases and Biotoxins that are useful to terrorists. It is combined with other apparatus that remove particulates and byproducts sometimes produced by the NUV source and maintains positive pressure of the confined space so as to prevent the influx of air from outside the protected zone.

A method and an apparatus for detecting and quantifying bacterial spores on a surface. In accordance with the method: bacterial spores are transferred from a place of origin to a test surface, the test surface comprises lanthanide ions. Aromatic molecules are released from the bacterial spores; a complex of the lanthanide ions and aromatic molecules is formed on the test surface, the complex is excited to generate a characteristic luminescence on the test surface; the luminescence on the test surface is detected and quantified.

The objective of the invention is to provide an improved method of making healthier juice products inoculated with probiotics, wherein the juice includes but is not limited to apple juice, coconut water, coconut milk, orange juice and/or carrot juice. The present invention relates to a method of pressure treating a juice containing probiotics. Vegetative cells of harmful microorganisms and enzymes are inactivated by applying high pressure while maintaining the activity of the probiotics. The juice preferably is dairy-free. The juice could be any dairy-free juice, such as a fruit juice, a vegetable juice, or their combination. Fruit and/or vegetable juice are prepared by any traditional methods, including but not limited to, washing, extracting by optionally treating with enzyme(s), centrifuging and packing. Probiotics, e.g., probiotics from bacterial spores, such as the spores of Bacillus coagulans and/or Lactobacillus plantarum, stay alive and active in the fruit or vegetable juice after high pressure pasteurization, as well as during the product shelf life.

The viscosity of 2-cyanoacrylate monomer based adhesives is increased by combining the monomers with suitable thickeners according to a certain process. The resulting formulations may be heat sterilized without degrading the viscosity or causing premature polymerization. The effectiveness of the sterilization process is assayed by disposing bacterial spores in the formulation, exposing it to a dry heat sterilization process, transferring it to a sterile aldose solution, transferring and exposing the sample to a nutrient medium which supports germination and growth of viable spores, incubating the samples, and determining the presence or absence of growth.

Provided herein are biologic sample preparation and analysis systems that are rapid, portable, robust detection system for multiplexed detection of bio-threats, and which can be ruggedized to operate in harsh environments. A new method of detection called Combinatorial Probe Analysis (CPA), which provides an exponential increase in detection reliability, has been incorporated into these systems. This type of analysis greatly reduces false positives and false negatives; in addition it is reusable and eliminates special storage requirements for reagents. Specific technical advancements in the optimization of hybridization assays for nucleic acid detection on porous polymer monoliths (PPM) are also disclosed. Performing rapid and complete solubilization of viruses, vegetative bacteria and bacterial spores with an ultra high temperature solubilization protocol is also described. The systems provided herein provides the ability to perform rapid highly multiplexed analysis of a variety of bioagents, including bacteria viruses, and protein biotoxins. The systems and assays described herein are perform completely automated sample preparation and analysis, in a time frame of five minutes or less. The assay is simple in design allowing users in personal protective equipment to easily operate the system. The disclosed systems are robust, simple to use, and address the goals of the first responder community.

Disclosed herein are therapeutic compositions containing non-pathogenic, germination-competent bacterial spores, for the prevention, control, and treatment of gastrointestinal diseases, disorders and conditions and for general nutritional health.

In one aspect, the present invention is directed to a plant or plant part coated with a composition comprising a bacterial spore and a germinative compound. In another aspect the present invention is directed to a method of enhancing the growth of a plant or plant part comprising coating such plant or plant part with such a composition. In yet another aspect, the present invention is directed to a composition comprising a bacterial spore and a germinative compound where such components are maintained in an inactive form. The present invention also relates to use of the compositions in wastewater treatment, environmental remediation, oil recovery, aquaculture systems, and direct fed microbials.