The invention relates to the technical field of urinary tract
infection detection, and discloses a phage-based rapid and accurate urinary tract
infection detection method, which comprises the following steps: S1, constructing a
CRISPR / Cas9
system; s2, constructing a homologous recombinant
plasmid; s3, constructing three plasmids BL21; s4, constructing, screening, verifying and reporting a phage T4:: Nluc; according to the detection method, a
CRISPR / Cas9
system is combined with a
homologous recombination technology, so that the efficiency of
bacteriophage gene editing is remarkably improved, based on the report
bacteriophage T4:: Nluc, rapid, sensitive, specific, accurate, simple and convenient detection on clinical urinary tract infection
escherichia coli is realized, and the method can be used for detecting an
escherichia coli urinary tract infection sample which is not pretreated, and has a wide application prospect. The kit shows excellent detection timeliness and specificity, is a powerful tool for identifying clinical urinary tract infection
escherichia coli, and provides a new technical path for timely and accurate diagnosis and
effective treatment of clinical urinary tract infection.