1725results about How to "Short detection cycle" patented technology

The disclosed electrochemical screening and early diagnostic device for malignant tumor comprises: an immune detection chip (1) connected to a time-resolution multichannel potentiostat (2) that connects to a data process and display system (3) by an interface. Wherein, the chip (1) comprises eight working electrodes fixed different antigen films and Ag wire (a), Ag/AgCl reference electrode (b), a carbon-pair electrode (c), and an insulation film (d). This invention needs low cost, has intelligence and fit to fast detection.

The invention provides a method for enriching target gene fragments. The method comprises the following steps: (1) obtaining a partial or whole-length DNA (deoxyribonucleic acid) sample library containing a target gene; (2) obtaining a DNA probe library capable of hybridizing with the target gene; (3) hybridizing the DNA probe library and the DNA sample library; (4) separating a hybrid product obtained in the step (3), and releasing the target gene fragments subjected to hybridizing and enriching. According to the enrichment method, the invention further provides a method for detecting the gene structure mutation of the target gene. The method for detecting the gene structure mutation of the target gene comprises the following steps: (1) enriching the target gene fragments according to the method; (2) detecting the structure mutation of the target gene. The target gene fragments enriched by using the method can be used for next-generation sequencing technique to detect the gene structure mutation and comprise single-alkali base mutation, mRNA (Messenger RNA) absence or increase, mRNA structure transversion and mRNA splicing change.

The invention provides a double-channel real-time bioaerosol monitoring method and a device thereof. The method is characterized by comprising the following steps: (1) concentrating atmospheric aerosol particles; (2) carrying out double-channel ultraviolet induced fluorescence detection; (3) data-processing; (4) cleaning the surface of an enrichment board of the particles and regenerating; and (5) repeatedly carrying out a new round of loop detection. The device comprises an aerosol-particles concentration unit, a double-channel ultraviolet induced fluorescence detection unit, a particle enrichment board regeneration mechanism, a rotary table and a control system. The invention can measure the density and the change of bio-particles such as bacteria, spores, viruses and the like in an ambient atmosphere in real time so as to achieve an early warning function on bioaerosol content in the atmosphere.

The invention discloses a method for determining the content of zinc in the zinc concentrate through energy-dispersive X-ray fluorescence spectrometry. The method comprises the following steps: 1, preparing a plurality of zinc-iron working liquids, converting the mass concentrations of Zn in the operating liquids to Zn mass percentage concentrations, detecting the Zn mass percentage concentrations with an energy-dispersive X-ray fluorescence spectrometer as a detector, recording the fluorescence intensity during each time injection of a sample, and making a working curve; and 2, processing the zinc concentrate as a sample to prepare a sample solution, injecting the sample solution under detection conditions for making the working curve, recording the fluorescence intensity displayed on the detector, reading out the Zn mass percentage concentration corresponded to the fluorescence intensity according to the determined working curve, and calculating according to a specific formula to obtain the mass percentage concentration of simple substance zinc in the sample. The method utilizes a solution process to eliminate or basically eliminate the matrix effect, so the zinc content can be accurately determined; and the method has the advantages of simple operation, short detection period, good detection result accuracy and high precision.

The invention discloses an inner detection system for submarine pipelines and a detection method thereof. The inner detection system comprises an electronic sealed cabin arranged in a supporting frame. The supporting frame is provided with a supporting roller array, an odometer wheel, a pressure sensor, a temperature sensor and a sound sensor, wherein the supporting roller array, the odometer wheel, the pressure sensor, the temperature sensor and the sound sensor are distributed in an annular mode. The detection method includes: the inner detection system is connected to an upper computer to download signals collected by the sensors, and filtering and noise reduction processing is performed on the signals; traveling mileage of the inner detection system and included angles of the axis of the inner detection system and a local geomagnetic field and local gravitational acceleration are calculated at any time t, and a direction vector of the axis of the inner detection system is obtained through the included angles; the position of the inner detection system at any time is obtained; and whether leakage exists is judged through comparison of processed sound signals, pressure signals and temperature signals, and leakage rate is estimated. The inner detection system for submarine pipelines and the detection method of the inner detection system achieve detection of little leakage, improve detection accuracy, reduce detection difficulty and cost, and shorten detection period.

The invention relates to a construction steel structure weld ultrasonic phased array detection technology. The technology employs an ultrasonic phased array detection instrument (1) and an ultrasonic phased array probe (2) to detect the weld defect (7) in a weldment's weld (6), and consists of the steps of: 1. instrument setting; 2. instrument calibration; and 3. scanning detection. Specifically the phased array detection instrument comprises a host, the probe, software, a scanning device and accessories. According to the detection technology provided by the invention, ultrasonic phased array is employed to scan the weld only 1 to 2 times so as to finish detection, and the detection result and image are stored. The technology can effectively shorten the detection cycle, can provide beneficial reference basis for ensuring safety assessment of construction steel structure, reduces the occurrence of safety accidents, and provides reference for design to reduce conservative design, enriches construction steel structure weld nondestructive detection methods, improves work efficiency and quality, and escorts construction steel structure welding quality.

The invention discloses a primary amino radical tester, which is obtained by improving a paradigm amino acid tester, is mainly used for measuring the primary amino radical content of proteins, and is popularized and application to the measurement of the primary amino radical content of various organic compounds. The tester mainly comprises a reaction part, a gas measuring part, a gas washing part and a recovering part, and has the characteristics of rapidness, accuracy, cleanliness and the like.

The invention relates to a method for detecting a release situation of nicotine in buccal tobacco products, which is characterized by comprising the steps of simulating the release situation of the nicotine in the buccal tobacco products in the human mouth, instantly quantifying the release quantity and the release speed and then detecting the release speed and the release degree of the nicotine in the buccal tobacco products. The release and the collection of the nicotine are realized through a release and collection device in vitro; by means of the device, the release process of the nicotine in the buccal tobacco products in the human mouth can be simulated and nicotine release solutions can be the instantly collected, wherein the release process is a process that the nicotine is continuously extracted from the buccal tobacco products by utilizing artificial saliva at a constant temperature; and the detection of the nicotine content in the release solution is realized through a high performance liquid chromatography. The method has the advantages: the release speed and the release degree of the nicotine in various buccal tobacco products can be rapidly and accurately detected with good repeatability, high sensitivity and short detection period and is suitable for the rapid analysis of mass samples for different industries and different purposes.

The invention discloses a method for detecting an acid value of edible fat based on near infrared spectrum analysis. The invention relates to a method for detecting an acid value of edible fat by utilizing near infrared spectrum analysis technology, which aims to solve the problems that in the practical production, the prior laboratory detection and test method can only perform intermittent operation, fail to realize accurate and quick on-line detection and the like. The method for detecting the acid value of the edible fat by utilizing the near infrared spectrum analysis technology is realized through the following steps of: 1, establishment of a calibration set sample spectrum; 2, pretreatment of spectrum data; 3, determination of essential data; 4, establishment of a calibration model; 5, verification of the calibration model; and 6, analysis of a sample to be tested. The method can effectively eliminate personal error, shorten detection period, and realize the on-line detection and control of the acid value in the process of processing the fat.

The invention pertains to the field of trace chemical substance detection, relates to a method and system for detecting whether special chemical substance is contained in the detected object. The method includes delivering sample of the detected object to a probe comprising nano-scale surface structure in order to make the sample in contact with the nano-scale surface structure; irradiating the nano-scale surface structure with a laser beam; scattering the laser beam by the sample and the nano-scale surface structure in order to generate a scattered light; obtaining the spectrum of the scattered light; analyzing the spectrum signal by a spectrum analyzer in order to detect whether special chemical substance is contained in the detected object. The invention provides simple and non-invasive detection method, which is convenient for carrying and easy to operate. The method and the system can be applied in fields of counter-terrorism, criminal investigation, medical diagnosis, disease prevention, industrial process monitoring, environment cleaning and monitoring, counterfeit goods detection, product identification, detection of methanol and ethanol content in alcohol, quality control of food and medicament or the like.

The invention discloses a bridge strain detection sensor based on a wireless sensing network interface. A data collecting module of the sensor is connected with a weak signal conditioning amplifier module, the weak signal conditioning amplifier module is connected with a data processing module, the data processing module is connected with a wireless transmission module, the wireless transmitting module is connected with a wireless receiving module, and finally the wireless receiving module is connected with a PC terminal; an ATmega 128L single chip in the data processing module is provided with an eight-channel tens-place ADC switching port, so that the strain detection sensor can detect the strain signals of eight channels, thus a CC 2420 radio frequency chip of the wireless transmitting module is used for carrying out wireless transmitting. And the signals are received by the wireless receiving module and then transmitted to the PC terminal by an SPI bus. The invention can detect the bearing capacity of the bridge and verify and develop the structural design of the bridge, and has the characteristics of no wiring, high detection efficiency, real-time detection, etc.

A detection method simultaneously detects residue of multiple synthetic antibacterial agents in aquatic products by a high performance liquid chromatography. The synthetic antibacterial agents mainly comprise sulfadiazine SD, sulfamerazine SMR, sulfadimidine SDD, sulfadimethoxine SDM, furazolidone FZD, oxolinic acid OXA, nalidixic acid NAA and the like. The multiple synthetic antibacterial agents in an aquatic product sample are extracted by acetonitrile, degreased by normal hexane, concentrated, purified by a solid phase extraction column (alumina neutral) or a C18 solid phase extraction column, analyzed by a liquid chromatograph, detected by an ultraviolet detector, and quantified by an external standard method. The method can quickly, sensitively and accurately detect multiple target compounds to greatly reduce detection cost and shorten detection time.

The invention discloses a pulse vibration high-frequency voltage injection-based six-phase permanent magnet synchronous motor sensorless control system and method, and belongs to the technical field of motor control. A band-pass filter is adopted to extract a high-frequency current signal comprising rotor position information; a low-pass filter is adopted to perform filtering on the extracted high-frequency current signal to obtain rotor error information; and a pulse vibration high-frequency voltage signal of a rotary coordinate system is injected into the motor, a stator winding current is sampled, and the position and the rotary speed of the motor rotor can be estimated from the high-frequency current correspondingly, so that quick, accurate and reliable initial position estimation on the six-phase permanent magnet synchronous motor rotor position can be carried out; and compared with the conventional back electromotive force method-based six-phase permanent magnet synchronous motorrotor position detection method, the method disclosed in the invention has relatively short detection period, simple realization and high practicability.

The invention discloses a detection integrated chip and a detection method. The chip is divided into an upper layer, a middle layer and a lower layer, wherein the upper layer of the chip is connected with the middle layer of the chip, the middle layer of the chip is connected with the lower layer of the chip; the upper layer or middle layer of the chip is provided with a group of through holes used for loading samples; the lower layer of the chip is provided with a group of grooves; and each layer of the chip is provided with micro-channels used for connecting the grooves. By adopting the chip, the pretreatment, quantitative delivery and reaction of the sample solution can be automatically completed once and the contents of the components of the solution can be detected, thus the detection of the multi-index single-reagent method can be satisfied and the chip can be used in the detection of the multi-index two-reagent method and the multi-reagent method. The chip disclosed by the invention can be used in the analysis and detection fields such as biological analysis, medical detection, environmental pollutant detection, and food and drug safety.

The invention relates to a method for detecting coaxiality and verticality of a large container by an optical instrument. The large petrochemical containers have common characteristics such as complicated structure, large geometric dimension and high manufacturing precision requirement. The method for detecting coaxiality and verticality of the large container by the optical instrument comprises the two steps of: (1) establishing a datum axis line by using a micro-alignment telescope, mounting a triangular centre bracket at a measured seal head, and reading an offset value of the bracket centre relative to the datum axis line by a micro-drumwheel; and (2) arranging a verticality measuring scale at the end face of the measured seal head, enabling an auto-collimation image to be completely superposed with a local image with an auto-collimation method when a reflector or a large reflection object in front of the telescope is vertical to the principal optical axis of the telescope, if the auto-collimation image is not superposed with the local image, adjusting the included angle between the large reflection object and the principal optical axis of the telescope to enable the auto-collimation image to be completely superposed with the local image, and computing the value of the verticality by using the scale value of a displacement graticule on the telescope and the read value of the micro-drumwheel. The invention is used for detecting the coaxiality and the verticality of the large container.

The invention discloses a method for screening 62 antibacterial drugs in livestock excrement. The method specifically comprises the following steps: (1) preparing a standard working solution; (2) preprocessing a sample to be detected before analyzing; (3) drawing a substrate matching standard curve; (4) performing mass spectrometric analysis on the sample containing a certain amount of drugs in step (3) so as to obtain standard molecular ions and two characteristic ions of each drug; wherein the two characteristic ions include qualitative ions and quantitative ions; (5) performing qualitative analysis; (6) performing quantitative analysis. The screening method is precise, accurate, and high in sensitivity; the technical support is provided to detect the residues of antibiotics in a livestock farm and the surrounding environment and evaluate the potential risk and the influence of the antibiotics residual in the excrement to the environment. Therefore, the method is high in social benefit.

The invention provides a POE switch testing device and a detection method thereof. The POE switch testing device comprises a temperature and humidity adjusting box; a POE switch system disposed in the temperature and humidity adjusting box; a POE system detection tool module connected with the POE switch system, arranged outside the temperature and humidity adjusting box, and used for detecting the power supply function of the POE switch system; an IP network camera connected with the POE switch system and arranged outside the temperature and humidity adjusting box; a temperature acquisition module connected with the POE switch system and used for detecting the temperature rise state of the POE switch system; and a PC arranged outside the temperature and humidity adjusting box, connected with the POE system detection tool module and used for detecting the data packet transmitting and receiving function of the POE switch system. The POE switch testing device solves the defects and the abuses of a conventional detection system using single AC voltage and power frequency by using different AC voltage and power frequency according to users at different regions.