327results about How to "Speed ​​up the breeding process" patented technology

The invention relates to the technical field of molecular markers, and discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to the litter size character of pigs and application of the SNP molecular marker. The SNP molecular marker is located at a 34977540bp site of a chromosome 2 of an international pig reference genome Sscrofa11.1 version, and the nucleotide sequence of the site is G or A and is located in an exon region of an LUZP2 gene. The invention also discloses an application of the SNP molecular marker in breeding of the litter size character of the boar, and provides a method for breeding or auxiliary breeding of the boar with high / low litter size based on the marker. The invention also provides a specific primer for identifying the SNP site. By applying the technologies, accurate prediction and efficient screening of the litter size of the large white pigs can be achieved, the breeding efficiency of excellent breeding pigs is remarkably improved, and the population improvement process is accelerated. The invention also provides important technical support for sustainable development of pig raising industry, also provides theoretical basis for research in related fields, and is expected to promote further development of pig genetic improvement work.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker site related to wheat cold resistance and application of the SNP molecular marker site. The molecular site is located at the 101st basic group from the 5'tail end of a sequence as shown in SEQ ID NO.1, and the polymorphism is C / T. The molecular marker related to wheat cold resistance in the wintering period provided by the invention can be used for detecting whether a wheat variety or strain contains a cold-resistant site or not, and has very high value for cold-resistant breeding of wheat, and meanwhile, the developed KASP molecular marker can be used for greatly accelerating the breeding process of the wheat cold-resistant variety.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to the testis size character of Hu sheep as well as a screening method and application of the SNP molecular marker, and belongs to the fields of genetic breeding and molecular biology. The marker is located in an intron region of a Hu sheep chromosome 3 LRRIQ1 gene and is remarkably related to the Hu sheep testis size character, and the accuracy and reliability of the SNP molecular marker are ensured through a scientific and rigorous screening method; according to the marker, Hu sheep individuals related to testis size characters can be rapidly and accurately screened out, the breeding efficiency and accuracy are improved, the breeding process is accelerated, the breeding cost is reduced, and the economic benefits are improved; the genetic diversity is protected, the accuracy of seminal fluid quality evaluation is improved, a powerful tool is provided for Hu sheep breeding work, and important basic data is also provided for researching a genetic mechanism of testis development and functions; the method can also be used as an auxiliary diagnosis tool to assist in identifying potential genetic defects or disease risks and promote progress and development of the breeding technology.

The invention discloses a peanut high-oil germplasm resource numerical prediction data model and a construction method and application thereof, and relates to the cross technical field of bioinformatics and peanut breeding. Obtaining peanut germplasm resources and / or peanut germplasm resource combinations with high genetic potential in high oil content characters on the basis of a pre-constructed peanut high oil germplasm resource numerical prediction data model, and further breeding high oil content improved varieties of peanuts. According to the method, early prediction of the oil content character of the peanuts can be realized, and excellent peanut germplasm with high oil content can be screened in the early planting stage of the peanuts, so that the peanut breeding period is shortened, the peanut breeding intensity is improved, and the breeding process of the high-oil-content germplasm of the peanuts is accelerated. The method has dual important values of scientific research and application.

The invention relates to the technical field of biology, and discloses application of a substance for inhibiting expression of an OsPP18 gene to improvement of rice blast resistance, and the OsPP18 gene is a gene for coding OsPP18 protein. An inoculation experiment shows that compared with receptor rice, the resistance of an OsPP18 knockout rice strain to rice blast is enhanced, and the resistance of an OsPP18 overexpression rice strain to rice blast is weakened, so that the OsPP18 is a gene related to plant disease resistance. The method disclosed by the invention is simple to operate and low in cost, greatly accelerates the breeding process, and has a wide application prospect.

The invention relates to a molecular marker which is located at the 47023718th basic group from the 5tail end on a No.16 chromosome of a pig reference genome Sscrofa11.1 and is related to a pig internal farrowing litter weight standard deviation. The invention describes a primer pair and a kit for detecting the molecular marker, and application of the primer pair and the kit in identifying the size of the standard deviation of the litter weight in the pig, screening pig varieties or genetic breeding of the pig. The pig internal litter weight standard deviation of the AA genotype individual is 2.79 kg, the pig internal litter weight standard deviation of the GG genotype individual is 4.38 kg, and the AA genotype individual is obviously smaller than the GG type individual, namely, the pig resilience of the AA genotype individual is higher than that of the GG individual. The AA type individuals are selected, and the pigs with small standard deviation of litter weight and high restoring force in the pigs can be obtained. According to the method, the problem of determining the pig resilience in actual production is effectively solved, the breeding cost is reduced, the pig internal litter weight standard deviation is effectively reduced, so that the pig resilience is improved, the method is high in accuracy and low in detection cost, automatic detection can be realized, and the method has very high practical application value in the aspect of pig breeding.

The invention discloses an application of a oryza longistaminata OlARF9 gene in improving hypoxia stress resistance of rice, and belongs to the field of bioengineering. According to the invention, overexpression is carried out on the oryza longistaminata OlARF9 gene in rice by adopting an overexpression technology, and according to phenotypic changes of transgenic plants, overexpression of the oryza longistaminata OlARF9 gene in the rice can significantly improve the ability of the rice to resist hypoxia stress, so that the oryza longistaminata OlARF9 gene can be applied to improve the resistance of the rice to abiotic stress and improve the resistance of the oryza longistaminata OlARF9 gene in the rice. Comprising the following steps: overexpressing the oryza longistaminata OlARF9 gene in rice to obtain a transgenic plant, wherein the resistance of the obtained transgenic plant to hypoxia stress is obviously enhanced. The method has application prospects in the aspects of improving and enhancing rice stress resistance, accelerating the stress-resistant molecular breeding process and the like.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to lactation traits of buffalo and application of the SNP molecular marker, the SNP molecular marker is located at the 51st basic group of Chr1166691407, the SNP molecular marker is G or T, and the nucleotide sequence of the Chr1166691407 is shown as SEQ ID NO.1. The invention further discloses a preparation method of the SNP molecular marker related to the lactation traits of buffalo and application of the SNP molecular marker related to the lactation traits of buffalo. The SNP molecular marker which is most related to the peak milk yield of the buffalo is obtained by means of DNA extraction and sequencing, genome comparison, whole genome association analysis and the like, and the peak milk yield of the GG type buffalo is superior to that of buffalo with other genotypes by researching the remarkable association between the genotype of the SNP molecular marker and the peak milk yield of the buffalo; the method is applied to auxiliary breeding of buffalo strains or varieties with the peak milk yield characteristic, so that the breeding period is shortened, the breeding efficiency is improved, and the buffalo produced by the bred buffalo with the peak milk yield characteristic can be greatly improved.

The invention belongs to the technical field of beef cattle molecular marker-assisted selection, and particularly relates to application of an SNP molecular marker related to the inclined length character of a beef cattle 6-month-old body. The SNP molecular marker is located at a basic group of a 71085551 site of a No.3 chromosome in a beef cattle genome GCF002263795.1 AR-UCD1.2 genomic.fna, the SNP site of the molecular marker has G / A polymorphism, and A is an unfavorable allelic variation of the oblique length character of the beef cattle at the age of 6 months. By detecting the base type of the SNP site of the molecular marker, the molecular marker is used for detecting or assisting in detecting the oblique length character of the beef cattle at the age of 6 months, genetic breeding of the oblique length character of the beef cattle at the age of 6 months is improved, and the breeding process of beef cattle varieties is accelerated.

The invention discloses SNP (Single Nucleotide Polymorphism) molecular markers of a Penaeus vannamei HIF-1alpha gene. The SNP molecular markers are respectively positioned at basic groups of the 67th site, the 119th site, the 133rd site, the 172nd site, the 340th site, the 409th site, the 437th site, the 474th site, the 577th site, the 647th site and the 749th site of a nucleotide sequence shown in a sequence 1 in a sequence table. The molecular marker is used as a functional marker of the nitrite-resistant character of the penaeus vannamei for breeding, specifically, genome DNA of muscular tissue of the penaeus vannamei to be detected is extracted, then the obtained genome DNA is used as a template for PCR amplification and purification of a PCR amplification product, then the obtained PCR amplification product is sequenced, and the content of the nitrite-resistant character of the penaeus vannamei is determined. The genotype of the molecular marker is determined, so that dominant genotype individuals are selected for breeding the penaeus vannamei boone, and a penaeus vannamei boone strain with good nitrite-resistant character can be obtained.

The invention discloses a molecular marker influencing the early weight of sheep as well as a primer and application of the molecular marker. According to the molecular marker capable of influencing the early weight of the sheep, two complete linkage sites of NC056056.1: g.26656G > A and NC056056.1: g.26473C > T of NR4A1 genes of the sheep, and three genotypes of GGCC, AGCT and AATT are shared. The weaning weight of the AGCT type sheep at the age of 45 days and the average daily gain from newborn to weaning are obviously higher than those of GGCC type sheep. The invention relates to a method for screening an early-stage rapid growth sheep variety (line), which comprises the following steps: detecting the G / A polymorphism of a NR4A1NC056056.1: g.26656 site and the C / T polymorphism of a NC056056.1: g.26473 site of sheep, and selecting AGCT type individuals for breeding. The polymorphic site can be used for early auxiliary selection of sheep growth traits, the selection accuracy is improved, and the breeding process is accelerated.

The invention discloses a canopy morphology 3D feature analysis method based on a lightweight unmanned aerial vehicle and point cloud singular value decomposition, and belongs to the field of plant morphology analysis. The method comprises the following steps: carrying out high-precision three-dimensional reconstruction on a soybean canopy structure by adopting an unmanned aerial vehicle to carry an RGB lens and combining with an SfM-MVS algorithm, introducing a singular value decomposition (SVD) method for a point cloud coordinate matrix based on a label method, and establishing nine canopy morphological 3D features under multiple scales as new explainable features of a crop plot level. Results show that the global canopy 3D features have significant correlation (plt; plt) with the yield, lodging, canopy height and vegetation index of soybeans; = 0.05). More detailed structure information of the canopy surface can be extracted based on 3D feature extraction of a local range, and characterization of differences of more growth periods of soybeans is facilitated.

The invention belongs to the technical field of wheat disease resistance breeding, and discloses a molecular marker for detecting wheat powdery mildew resistance QTL and application of the molecular marker. On the basis of a three-year group powdery mildew adult-plant stage resistance identification result, the powdery mildew resistance QTL is detected through a complete interval mapping method (ICIM) of IciMapping v4.2, two major QTLs, namely Qpm.histwrc-1AS and Qpm.histwrc-2BS-1, are detected in total, and the resistance contribution rates are 19.4%-34.5% and 19.7%-21.3%. Related molecular markers are developed through Bainong dwarf 58 sequencing data and can be applied to wheat disease-resistant molecular breeding, and a foundation is laid for exploring an Edinburgh-B disease-resistant gene and a resistance genetic mechanism.

The invention belongs to the field of molecular genetic breeding, and particularly relates to a KASP molecular marker method related to peanut aspergillus flavus seed kernel infection resistance and application of the KASP molecular marker method. Through whole genome association analysis, the SNP locus is located at the 133198521th bp position of the peanut B06 chromosome (the reference genome is peanut), the nucleotide sequence polymorphism is C / A (the antisense strand is G / T), and the SNP locus is named as qARIB06. Based on the SNP site, KASP molecular marker primer sequences (SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3) are designed and developed, and the KASP molecular marker primer sequences are used for rapidly and accurately identifying the genotype of a peanut sample.

The invention discloses an OsUBC46 protein related to plant disease resistance and yield increase as well as a related biological material and application of the OsUBC46 protein. The OsUBC46 protein can be specifically a protein of A1), A2) or A3) as follows: A1) a protein of which the amino acid sequence is SEQ ID No.2 in a sequence table; a2) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues of the protein of A1), has 75% or more of identity with the protein of A1) and has the activity of regulating and controlling plant disease resistance and yield increase; and A3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1) or A2). The OsUBC46 protein and related biological materials thereof can be used for regulating and controlling the disease resistance and yield increase of plants.

The invention discloses a rapid screening method for high-bait-efficiency character haliotis discus hannai. The rapid screening method for the high-bait-efficiency character haliotis discus hannai can be achieved through any one of a mode I and a mode II. Mode I) selecting 12-month-old haliotis discus hannai to perform constant-temperature single-individual breeding, performing hunger stress for 7 days, calculating the specific weight loss rate of each individual after the hunger stress for 7 days, and selecting 50% of individuals with relatively low specific weight loss rate as individuals with relatively high bait efficiency. And II) selecting 12-month-old haliotis discus hannai to carry out constant-temperature single-individual culture, carrying out starvation stress for 10 days, then recovering bait feeding to 28 days, recording the weight change condition of the haliotis discus hannai, calculating the weight gain of each individual in 14-28 days after the bait feeding is recovered, and taking 50% of individuals with higher weight gain as individuals with higher bait efficiency. According to the screening method provided by the invention, the haliotis discus hannai individuals with relatively high bait efficiency characters can be accurately and quickly obtained, the evaluation period is shortened, and the character evaluation cost is greatly reduced.

The invention relates to the technical field of rice molecule-assisted breeding, in particular to a molecular marker of major QTL qCST3 for regulating and controlling rice salt tolerance and application of the molecular marker, major QTL is located on a rice chromosome 3 and named as qCST3, and the physical position is 30.48 Mb to 32.90 Mb; the SNP marker closely linked with the SNP marker is located on the 33887916 site of the chromosome 3, and when the position is C, the rice is indicated to be salt-tolerant rice; and when T is equal to T, indicating that the rice is salt-sensitive rice. The novel major QTL and the linked molecular marker thereof provided by the invention can be used for quickly judging the salt tolerance level of rice, so that the rice variety screening and breeding process is accelerated.

The invention discloses a molecular marker C153 related to growth traits of portunus trituberculatus and application of the molecular marker C153. The nucleotide sequence of the molecular marker C153 is as shown in SEQ ID NO. 1, and the sequences of primers for detecting the molecular marker C153 are as shown in SEQ ID NO. 2 and SEQ ID NO. 3. The molecular marker C153 is an SNP (Single Nucleotide Polymorphism) marker, and the growth speed of an individual with the 115th genotype of the molecular marker C153 being GG is obviously higher than that of a portunus trituberculatus individual with genotypes of AG and AA. The molecular marker C153 provided by the invention can be used for accelerating the breeding speed of the growth traits of the portunus trituberculatus, saving a large amount of manpower and material resources, promoting the process of breeding improved varieties of the portunus trituberculatus, being beneficial to the healthy and sustainable development of the portunus trituberculatus industry and having a wide market application prospect.

The invention belongs to the technical field of crop breeding, and particularly relates to a construction method and application of a wheat rapid generation adding system, the construction method comprises the following steps: S1, taking young ears after flowering of wheat crops, stripping seed immature embryos, disinfecting and sterilizing, then inoculating the seed immature embryos into an induction culture medium, and performing dark culture to germinate the seed immature embryos to obtain seed immature embryos; then culturing under illumination until embryo seedlings grow; s2, transplanting the embryo seedlings into a planting pot, and performing seedling recovery or vernalization treatment; s3, transferring the embryo seedlings into an artificial climate chamber, and culturing until the plants bloom; wherein the temperature and illumination conditions are as follows: in a seedling stage, darkness at 20 DEG C is performed for 6 hours, illumination at 25 DEG C is performed for 18 hours, and the operation is performed alternately From the jointing stage to the mature stage, darkness at 22 DEG C is performed for 6 h, illumination at 28 DEG C is performed for 18 h, and operation is performed alternately. According to the construction method disclosed by the invention, the breeding time and the growth cycle of the wheat crops are greatly shortened, meanwhile, normal development and fruiting of the crops are ensured, and the breeding process of good varieties is greatly improved.

The invention discloses a camellia oleifera SNP (Single Nucleotide Polymorphism) molecular marker combination and application thereof. The camellia oleifera SNP molecular marker combination comprises at least one of 50 SNP molecular markers and 454 SNP molecular markers, and the physical positions of the 50 SNP molecular markers and the 454 SNP molecular markers are determined on the basis of sequence alignment of a camellia oleifera reference genome GCA039905865.1. The camellia oleifera SNP molecular marker combination can be effectively used for molecular marker breeding of camellia oleifera, the camellia oleifera SNP molecular marker combination is used for preparing a first type of whole genome liquid phase chip of camellia oleifera, and the application cost of genetic diversity analysis, QTL positioning, GWAS analysis and the like in scientific research of camellia oleifera is effectively reduced; the problem that no applicable product exists in camellia oleifera molecular auxiliary breeding and whole genome selective breeding is solved, and the process of camellia oleifera basic research and breeding is accelerated.

The invention belongs to the technical field of biology, and particularly relates to a barley grain weight related gene HvTGW1s and application thereof. The invention discloses a barley grain weight related gene HvTGW1s, the base sequence of the barley grain weight related gene HvTGW1s is shown as SEQ ID NO: 1, and the barley grain weight related gene HvTGW1s has the function of increasing the thousand grain weight, grain width and grain length of barley. The invention further discloses the KASP marker associated with the barley yield character and application of the KASP marker in barley yield assisted selective breeding. The invention further discloses a kit for assisting in identifying the barley yield. The method is applied to barley yield assisted selection breeding, the breeding efficiency can be effectively improved, and the breeding process is accelerated.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to the tolerance character of white spot syndrome virus (WSSV) of procambarus clarkia, the molecular marker is located in the third intron region of a TRIM gene, is specifically located at 585bp of a DNA (Deoxyribose Nucleic Acid) sequence as shown in SEQ ID NO.1, and is Tgt; the T / C heterozygous genotype is obviously related to the WSSV tolerance character of the procambarus clarkia. The invention provides a favorable marker for breeding of disease-resistant molecules of procambarus clarkii, and can accelerate the breeding process of disease-resistant varieties of procambarus clarkii.

The invention relates to a primer group of a microsatellite marker HA223 related to growth of belly-expanding hippocampus and application of the primer group, and belongs to the field of molecular biology, and the sequence of the primer group is shown as SEQ ID NO.1-2. The invention also provides an application of the primer group in breeding of growth traits of hippocampus erectus, the primer group is utilized to perform PCR amplification on DNA of a hippocampus erectus individual, an individual which can amplify a specific fragment with the size of 260bp is screened as a basic parent, and a heterozygous individual or a homozygous individual with the fragment is produced and used for breeding of a rapid growth variety, so that the breeding of the growth traits of the hippocampus erectus is facilitated, and the breeding efficiency of the hippocampus erectus is improved. Compared with a conventional breeding method, the method can shorten the breeding period, accelerate the breeding process and improve the breeding efficiency.

The invention discloses a method for accelerating a rice breeding process based on LED illumination, and belongs to the technical field of agriculture. According to the precise regulation and control method based on LED illumination, an LED spectrum combination and photoperiod scheme suitable for different growth stages of rice is designed, in combination with optimization of illumination intensity, uniformity and dynamic spectrum, rapid growth, blooming and fruiting of plants are effectively promoted, the photosynthesis efficiency is improved, high-yield and high-quality rice cultivation is finally achieved, and the method is suitable for large-scale popularization and application. Through dynamic spectrum regulation and control, accurate temperature and humidity adjustment and multi-generation rapid screening processes, the growth cycle of rice is greatly shortened, meanwhile, accurate nutrition supply is combined, plants with target characters are rapidly screened out, the breeding efficiency is improved, and through linkage optimization of illumination, temperature and humidity and nutrition regulation and control and combination of an automatic irrigation technology, the breeding efficiency is improved. Manual intervention and management errors are remarkably reduced, the environment control precision is improved, and the method has high adaptability and expansibility and can be widely applied to phytotron planting of other crops.

The invention belongs to the technical field of molecular biotechnology and molecular markers, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker located on a porcine chromosome 5 and related to a live litter size character and an application of the SNP molecular marker. The SNP site of the SNP molecular marker located on the pig chromosome 5 and related to the live litter size character corresponds to the Tgt at the 89503311bp position on the chromosome 5 of the international pig reference genome version 11.1; g mutation. Whole genome correlation analysis and detection of the molecular marker show that the molecular marker significantly affects the character of the number of live piglets born by pigs. The invention also provides a primer pair and a kit for identifying the molecular marker, an efficient and accurate molecular marker assisted breeding technology can be established by utilizing the molecular marker, the primer pair or the kit, and the molecular marker assisted breeding technology is applied to the character genetic improvement of the live birth number of the boar, so that the live birth number of the boar is increased, the profit of an enterprise is increased, and the core competitiveness is improved.

The invention relates to an LRRK1 gene SNP (Single Nucleotide Polymorphism) molecular marker related to a duck slaughter rate and application thereof, and belongs to the technical field of molecular biology. Whole genome association analysis is utilized to discover that an SNP locus related to the growth traits of meat ducks exists on the NC040056.1 chromosome of the melanmen ducks for the first time, the locus obviously influences the 10-week-old dressing percentage of the melanmen ducks, and a TT genotype shows an excellent genotype. Therefore, a pair of upstream and downstream primers is provided for amplifying the flanking sequence of the molecular marker, the molecular marker is subjected to genetic typing through the flanking sequence, and molecular marker-assisted selection is implemented according to the typing result. A reliable way is provided for breeding or cultivating local sheldrake varieties with relatively high growth speed and relatively high dressing percentage, and the breeding process of meat ducks is accelerated.

The present invention discloses a rice Wx mutant protein, a mutant gene and its application, and also discloses a breeding method for creating quality-improved rice with transparent appearance and appropriately reduced amylose content by gene editing. The present invention uses gene editing technology for the first time to Wx By editing the gene with a single base, and through the screening of the offspring, new materials with T-DNA removed, transparent appearance, appropriately reduced amylose content, improved quality, and stable inheritance can be obtained in the T2 generation. Compared with breeding such as chemical mutagenesis and hybridization, gene editing directed improvement molecular breeding technology has the advantages of rapidity, accuracy, and efficiency. Combined with gene function marker genotype selection, or directly using other mature rice varieties as background materials for transformation and obtaining corresponding materials, it will greatly improve breeding efficiency and greatly accelerate the breeding process.

The invention relates to the field of molecular biology and genetic breeding, and provides application of an SNP (Single Nucleotide Polymorphism) marker related to a body height character. The SNP marker is located at the 136651710 bp position of the No.3 chromosome of sheep, and is Cgt; and T base mutation (rs403130818) is carried out. Experiments show that the SNP marker developed by the invention has significant influence on the sheep body height character (Plt; 0.05), and the allele T has more excellent body height character, so that the SNP molecular marker in the MCRS1 gene can be applied to molecular marker breeding of the sheep body height character. The molecular marker provided by the invention is not limited by the age, sex and the like of the sheep, can be used for breeding excellent body height sheep varieties, and even can be accurately screened when the sheep are newly born, so that the breeding process of the excellent body height sheep varieties is greatly accelerated.

The invention discloses a phenotype platform omnibearing self-adaptive acquisition device and method, and the device comprises an assembly line type phenotype platform which comprises a platform frame and a conveying belt, the platform frame is provided with a platform inlet, a platform outlet and a detection area, and the conveying belt penetrates through and is located below the platform frame; the plant feature recognition mechanism is arranged in the detection area and located on the side directly facing the conveying belt; the omni-directional self-adaptive acquisition mechanism is arranged in the detection area and comprises an omni-directional rotary part, an acquisition height adjusting part and a phenotype self-adaptive acquisition part, and the omni-directional rotary part corresponds to the plant feature recognition mechanism and is mounted at the top of the platform frame; the acquisition height adjusting component is respectively connected with the all-directional rotary component and the phenotype self-adaptive acquisition component; and the automatic control mechanism is respectively connected with the assembly line type phenotype platform, the plant feature recognition mechanism and the all-dimensional self-adaptive acquisition mechanism. The invention also discloses a phenotype platform omnibearing adaptive acquisition method.

The invention belongs to the technical field of molecular biotechnology and molecular markers, and discloses a pig nipple number character related SNP molecular marker and application thereof, the site of the SNP molecular marker corresponds to the nucleic acid site at the 103001352bp site of the No.7 chromosome of the international pig reference genome Sscrofa11.1 version sequence, and the site is Ggt; and A basic group mutation. The SNP molecular marker associated with the nipple number character of the large white pig can be applied to genetic improvement of the growth and reproduction character of the large white pig, provides a new genetic marker resource for molecular marker-assisted selection of the nipple number character of the large white pig, can be applied to breeding of sows with high lactation capacity and high piglet survival rate, accelerates the breeding process, and improves the breeding efficiency. The reproductive performance of offspring large white pigs is improved.