37results about How to "Speed ​​up the breeding process" patented technology

The invention relates to the field of molecular biology and genetic breeding, and provides application of an SNP (Single Nucleotide Polymorphism) marker related to a body height character. The SNP marker is located at the 136651710 bp position of the No.3 chromosome of sheep, and is Cgt; and T base mutation (rs403130818) is carried out. Experiments show that the SNP marker developed by the invention has significant influence on the sheep body height character (Plt; 0.05), and the allele T has more excellent body height character, so that the SNP molecular marker in the MCRS1 gene can be applied to molecular marker breeding of the sheep body height character. The molecular marker provided by the invention is not limited by the age, sex and the like of the sheep, can be used for breeding excellent body height sheep varieties, and even can be accurately screened when the sheep are newly born, so that the breeding process of the excellent body height sheep varieties is greatly accelerated.

The invention discloses a method for cultivating a heat-resistant broad-spectrum durable rice blast-resistant rice breeding material based on polygene polymerization. According to the method, a high-heat-resistance and high-dominance indica-japonica hybrid indica rice restorer line is selected as a recurrent parent, a breeding intermediate material for polymerizing complementary broad-spectrum rice blast resistance genes Pigm and Pi-jx is used as a donor parent, and polymerization of the heat-resistance and broad-spectrum lasting rice blast resistance genes Pigm and Pi-jx is realized through a method of combining molecular breeding with conventional breeding. Meanwhile, by selecting representative strains to carry out artificial inoculation identification of seedling blast and panicle blast, natural induction identification of a rice blast retransmission area and heat resistance identification, and by combining main agronomic trait evaluation in the whole growth period, a heat-resistant broad-spectrum durable rice blast-resistant breeding material is obtained. The breeding material bred by using the method disclosed by the invention has relatively strong heat resistance and broad-spectrum and lasting rice blast resistance, and the breeding process of heat-resistant and rice blast-resistant rice varieties is accelerated.

This invention relates to the fields of molecular markers and animal genetic breeding technology, specifically to a molecular marker located on chromosome 4 of pigs that is associated with body size and carcass traits, and its application. Using the F7 generation of a chimeric family as the research object, this invention employs whole-genome resequencing and GWAS analysis to study and identify the molecular markers located on chromosome 4 of pigs associated with body size and carcass traits. By optimizing the dominant alleles of these molecular markers, the frequency of dominant alleles can be increased generation by generation, enabling rapid and accurate selection of body size and / or carcass traits, accelerating the progress of pig genetic improvement, and thus effectively improving the economic benefits of pig breeding.

The invention relates to the technical field of biology, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker related to soybean mosaic virus resistance, an amplification primer and application of the SNP molecular marker in soybean breeding. The SNP molecular marker is located at the 910489bp site of a No.7 chromosome of a soybean genome, and the polymorphism of the SNP molecular marker is C / A. The invention provides application of the SNP molecular marker related to the soybean mosaic virus resistance in identification or auxiliary identification of the soybean mosaic virus resistance. By providing the accurate, simple, convenient and low-cost SNP molecular marker and the detection method thereof, early-stage, rapid and efficient molecular-assisted selection of soybean mosaic virus resistance characters is realized, so that the breeding process of a new variety of disease-resistant soybeans can be remarkably accelerated, and the SNP molecular marker has important breeding application value.

The invention provides a molecular marker, a detection reagent and a method for evaluating growth traits and / or egg laying interval traits of meat pigeons. The SNP locus and the haplotype molecular marker which are remarkably related to the body size character, the body weight character and / or the egg laying interval character of the meat pigeon are screened from the first intron of the ASIP gene of the meat pigeon, and can be applied to early auxiliary selection of meat pigeon breeding. The invention further provides a detection reagent and a detection method for detecting the SNP loci and the haplotype molecular markers, the body size character, the body weight character and / or the egg laying interval character of the meat pigeons can be quickly, effectively and accurately predicted, so that the production cost is saved, the breeding process is accelerated, and the detection reagent and the detection method have high economic application value and scientific research value.

The present application relates to the technical field of plant genetic engineering, and in particular to a circular RNA PacircCNGC of Polygonatum cyrtonema and application thereof.The cDNA nucleotide sequence of the circular RNA PacircCNGC is shown in SEQ ID NO.1 or SEQ ID NO.2.The present application finds the role of the circular RNA PacircCNGC of Polygonatum cyrtonema in plant drought resistance and salt resistance, provides a theoretical basis and gene resource for resistance breeding of Polygonatum cyrtonema, provides a powerful tool for transgenic research of Polygonatum cyrtonema, provides a valuable circular RNA for molecular breeding of Polygonatum cyrtonema, and has important significance for revealing the molecular mechanism of bamboo resistance, breaking through the limitations of conventional breeding, and accelerating the breeding process of bamboo.

The application of broccoli genes BoSULTR1;1 and BoSULTR1;2 in improving plant stress resistance belongs to the technical field of plant genetic engineering. In order to solve the technical problem of how to improve the stress resistance of broccoli plants, the application provides the broccoli genes BoSULTR1;1 and BoSULTR1;2, and the application uses an expression vector or a recombinant bacterium overexpressing BoSULTR1;1 and BoSULTR1;2 genes to prepare a transgenic broccoli plant. The stress resistance related detection results prove that the BoSULTR1;1 and BoSULTR1;2 genes have the ability to improve the resistance of the transgenic broccoli plant to cotton bollworm, pathogenic bacteria and drought. The transgenic material obtained by the application has important theoretical significance and practical value for accelerating the breeding process of stress-resistant plants and improving the breeding efficiency.

The invention belongs to the field of fish genetic breeding, and particularly relates to a method for preparing sterile triploid fish with a tyr gene knocked out, which comprises the following steps: S1, knocking out wild type female diploid red crucian carp and male autotetraploid crucian carp high mutants through the tyr gene, and screening out the female diploid red crucian carp high mutant and the male autotetraploid crucian carp high mutant; and S2, hybridizing the female diploid red crucian carp high mutant and the male autotetraploid crucian carp high mutant to prepare the tyr gene knockout sterile triploid crucian carp homozygous mutant on a large scale. By utilizing the method, the triploid fishes with the accurately improved characters are prepared on a large scale, and the problem of biological safety is solved due to the natural sterility characteristics of the triploid fishes, so that the method has important practical value and ecological significance for genetic breeding and sustainable development in aquaculture.

The invention discloses an SNP molecular marker related to grass carp feed utilization rate and application thereof, and belongs to the technical field of aquatic animal molecular markers and aquatic genetic breeding. According to the invention, an SNP (Single Nucleotide Polymorphism) site obviously related to the feed utilization rate of the grass carp is screened out, the SNP molecular marker is positioned at the 51653399 site on the chromosome 2 of the grass carp, the polymorphic site of the SNP molecular marker is G / T, and when the genotype of the site is GG, the feed utilization rate of the grass carp is relatively high. The invention also discloses application of the SNP molecular marker, the amplification primer or the kit in breeding of grass carp with high feed utilization rate. The SNP molecular marker provided by the invention can be used for early breeding of grass carp with high feed utilization rate, greatly reduces the breeding workload, can significantly shorten the breeding time, accelerates the breeding process, improves the breeding efficiency, and reduces the breeding cost.

The invention relates to the technical field of biology, in particular to a KASP molecular marker for tobacco drought resistance characters and application of the KASP molecular marker. The molecular marker c22-1 and the molecular marker c22-4 which are closely linked with the tobacco drought resistance character are finally obtained by carrying out a first round of screening on the tobacco drought resistance character gene based on sequencing data and then carrying out a second round of large-scale screening on the tobacco drought resistance character gene by utilizing a KASP technology. The molecular marker is used for breeding the drought-resistant tobacco variety, the detection result stability is good, the repeatability is high, the detection result consistency is good, a more accurate scientific basis can be provided for subsequent gene function research and breeding work, and the breeding process of the drought-resistant tobacco variety is accelerated.

The application discloses a haplotype related to cotton fiber strength and a functional KASP molecular marker and detection application thereof, and relates to the technical field of plant molecular breeding and genetic markers. The haplotype is located in a 5,980,849-5,982,917 bp segment of a cotton reference genome D08 chromosome, is composed of 7 SNP sites, and can be divided into two types of Hap1 and Hap2. The KASP molecular marker is located at 5,982,917 bp of the cotton reference genome D08 chromosome, and the base is A / T. The haplotype represented by the molecular marker is significantly related to the cotton fiber strength, the KASP detection primer developed based on the haplotype has the advantages of accurate detection, simple operation, high throughput and low cost, and can be used for screening of early-stage materials in cotton breeding, significantly improves the selection efficiency of the fiber strength, and speeds up the breeding process of new cotton varieties with high quality.

The invention discloses a Camel hoof valve gene ZbBCAT2 and application thereof to improvement of salt tolerance of plants. The CDS sequence of the Camel hoof valve ZbBCAT2 gene is as shown in SEQ ID NO.1, and the coded amino acid sequence is as shown in SEQ ID NO.2. The invention further discloses a preparation method of the Camel hoof valve gene ZbBCAT2. The invention also discloses an application of the Camel hoof valve gene ZbBCAT2 in improving the salt tolerance of plants. The overexpression of the Camel hoof valve ZbBCAT2 gene plays a negative regulation role in the salt tolerance. The Camel hoof valve ZbBCAT2 gene provided by the invention can be used as a candidate gene for breeding the salt-resistant new variety of the plant, and has important significance for cultivating the salt-resistant new variety of the plant by adopting a genetic engineering method, accelerating the breeding process and reducing the breeding workload.

This invention relates to the fields of molecular biology and genetic breeding, and particularly to the dCAPS marker associated with the length of adventitious roots in wheat under waterlogging stress and its application. This dCAPS molecular marker is based on the 234,677,747th base of wheat chromosome 6B; the SNP base difference is either C or T. This molecular marker is accurate and efficient to detect, convenient and stable to amplify, and can be used for marker-assisted selection to improve the efficiency and accuracy of identifying the waterlogging stress resistance of wheat plants.

This invention discloses a method for promoting somatic embryogenesis and maturation in Cryptomeria japonica, belonging to the field of somatic embryogenesis technology. The method involves using immature seed embryos as explants to induce embryogenic callus; transferring the proliferated embryogenic callus to a somatic embryogenesis induction medium for further culture to obtain somatic embryos; transferring the somatic embryos to a somatic embryogenesis maturation medium for dark culture to obtain mature cotyledonary embryos; transferring the mature cotyledonary embryos to a somatic embryogenesis basal medium for light culture to obtain somatic embryonic seedlings; and hardening off the seedlings before transplanting to obtain regenerated plants. The results of this application show that embryogenic callus can be stably obtained from different families, with high somatic embryogenesis maturation capacity and rate, enabling the cultivation of a large number of high-quality seedlings with stable genetic traits in a short time; avoiding the phenotypic segregation problem caused by gene recombination during seed propagation, and ensuring that offspring plants inherit the superior characteristics of the parents.

This invention belongs to the field of plant genetic engineering, specifically relating to an isolated nucleic acid molecule encoding the Sapindus mukorossi SmHD-ZIP34 protein and its application. The isolated nucleic acid molecule has a nucleotide sequence as shown in SEQ ID NO.1, or encodes an amino acid sequence as shown in SEQ ID NO.2. This invention reveals for the first time the dual function of this isolated nucleic acid molecule in simultaneously regulating flowering time and sex differentiation in plants. Experiments have shown that overexpression of this nucleic acid molecule in Arabidopsis thaliana significantly promotes flowering and pistil elongation, and inhibits stamen development by downregulating the core pathway of stamen development, leading to a reduction in the number of stamens and pollen abortion. The nucleic acid molecule resource provided by this invention offers an effective solution to the problems of long breeding cycles and low fruit set rates due to male flower dominance in economic forest trees such as Sapindus mukorossi, and can be used to cultivate high-yielding new germplasm with early flowering, fewer males, and more females.

The application discloses a parent-offspring identification primer group and method of Prunus. The parent-offspring identification primer group of Prunus disclosed by the application is composed of 30 single-stranded DNAs shown in sequence 1 to sequence 30 in a sequence list. The primer group and method of the application can identify the genetic relationship of Prunus armeniaca, Prunus dulcis and their offspring, establish a parent-offspring identification system, and are simple to operate. The primer group and method of the application can quickly obtain genetic information of Prunus parents and offspring and determine the source of the offspring, can early determine and retain hybrid offspring between Prunus armeniaca and Prunus dulcis at the seedling stage, which is beneficial to quickly determine the true outcrossing seedling at the seedling stage, thereby removing hybrid offspring caused by natural pollination and eliminating the hybrid offspring at the seedling stage, so as to save land resources, reduce the identification cost in the later period, speed up the breeding process, and also provide a reference for parent-offspring identification of other fruit plants.

This invention relates to the field of poultry breeding technology, specifically to a breeding method for a high-breeding, fast-growing, dwarf, slow-feathering Guangxi Ma chicken. The method includes: constructing a molecular detection system for the sex-linked dwarf gene GHR and the recessive white-feathered RWC gene; hybridizing Guangxi Ma chicken breeding hens with dwarf, recessive white-feathered breeding roosters to obtain a hybrid F1 generation; self-pollinating the F1 generation to produce the F2 generation; using phenotypic selection combined with molecular detection technology to screen out dwarf, slow-feathering Guangxi Ma chicken individuals in the F2 generation; subsequently selecting breeding roosters and hens to form a core group of dwarf, slow-feathering Guangxi Ma chickens; establishing a Generation 0 family; and through successive generations of selection to develop a new high-breeding, fast-growing, dwarf, slow-feathering Guangxi Ma chicken strain. The dwarf, slow-feathering Guangxi Ma chicken strain bred by this invention inherits the dwarf characteristics of dwarf, recessive white-feathered chickens, has a lower feed intake during the laying period compared to Guangxi Ma chickens, and increases egg production, fertilization rate, and egg quality rate, effectively reducing breeding costs; simultaneously, the use of gene detection technology improves the accuracy and efficiency of breeding.

The invention discloses application of an InDel molecular marker located on a pig chromosome 16 and related to nipple number heterosis, the locus of the InDel molecular marker is rs793470896, and the InDel molecular marker corresponds to GGAGGCACgt at the 74359589 bp position on the chromosome 16 of an international pig reference genome version 11.1; the gene type of the InDel molecular marker is GGAGGCAC / GGAGGCAC, GGAGGCAC / G or G / G. The gene type of the InDel molecular marker is GGAGGCAC / GGAGGCAC. The InDel molecular marker provided by the invention is used for marker-assisted selective breeding, pigs with the genotype of GGAGGCAC / GGAGGCAC in a group are gradually eliminated, and the nipple number of bred sows can be remarkably increased, so that the piglet breeding and lactation capabilities of the sows can be remarkably improved, the survival rate of piglets can be increased, and the production income can be increased.

This invention discloses a rice blast resistance gene. Pilx SNP molecular markers, primer sets, and applications, wherein the SNP molecular markers are... Pilx The SNP molecular marker Pilx The polymorphic site is located on rice chromosome 12. Pilx The 18066332nd base of the gene is either T or C. This invention relates to a rice blast resistance gene. Pilx SNP molecular markers were used, and PARMS technology was applied to the reaction of rice materials. Pilx Genetic testing can rapidly and accurately detect rice blast resistance genes in different germplasm resources such as indica and japonica rice. Pilx .

This invention discloses SSR marker primers specific to the filamentous petals of gerberas and their applications. This invention utilizes SSR molecular markers to effectively identify different filamentous petal traits in gerberas, resulting in an SSR marker primer that can rapidly and accurately identify the petal traits of gerbera plants with different filamentous petals and the F1 generation of interspecific hybrids. The SSR marker primers provided by this invention can effectively distinguish between filamentous and non-filamentous petals in gerberas and exhibit significant polymorphism, with an accuracy greater than 65%. Furthermore, compared with traditional morphological trait observation methods, the identification method provided by this invention has better repeatability, saves time, and has high specificity. It can replace traditional morphological identification methods, effectively improving the molecular-level breeding process of gerberas and reducing the identification cycle of traditional morphological trait observation. It also allows for early screening to obtain gerbera offspring with superior filamentous varieties, which is of great significance for gerbera breeding.

This invention discloses a SNP locus associated with wheat salt tolerance and its application in predicting wheat salt tolerance, belonging to the field of molecular marker breeding technology. The SNP locus of this invention is located at position 1655 of the CDS region of the TaHKT1;1-B gene. When the base at this locus is G, the corresponding wheat is salt-tolerant; when the base at this locus is A, the corresponding wheat is not salt-tolerant. Based on this SNP locus, a method for predicting wheat salt tolerance has been developed, which can predict the maximum relative root length of wheat after salt stress, saving costs, improving selection efficiency, and thus accelerating the breeding process. This provides new potential opportunities for efficient screening of superior alleles and the breeding of salt-tolerant and drought-resistant wheat varieties.

The application relates to the fields of molecular biology and genetic breeding, and provides an application of a SNP molecular marker related to a body height trait of sheep. The SNP molecular marker is located at 4864599 bp of a sheep chromosome 16, and is a G>A base mutation (rs404408103). Experiments show that the SNP molecular marker developed in the application has a significant influence on the body height trait of sheep P <0.01>, and the allele A has a more excellent body height trait, so ERGIC1 The SNP molecular marker in the gene can be applied to sheep body height trait molecular marker breeding. The molecular marker provided by the application is not limited by the age and gender of the sheep, can be used for breeding excellent body height sheep varieties, and can be accurately screened even after birth, so that the breeding process of the excellent body height sheep varieties is greatly accelerated.

The present application relates to a kind of molecular markers Pv_0026128 closely linked with kidney bean pod length gene and its KASP primer sequence, the marker is located at the position of 19213953bp on chromosome 1, the identified material can be amplified using the marker, whether the sample carries the allelic variation of controlling pod length is judged according to its genotype.Use the molecular marker and primer provided by the present application can realize the auxiliary screening of kidney bean pod length trait, high-throughput detection is carried out in short time, compared with traditional phenotype-based selection, selection result is more accurate, it is convenient to quickly screen out kidney bean variety or strain with pod length, improve kidney bean yield.Combined with the breeding method of KASP also greatly improves breeding efficiency, thereby speeds up the breeding process of kidney bean.

This invention discloses a structural variation molecular marker on chromosome 5 of pigs that affects the weight of subcutaneous abdominal fat and its application. The structural variation molecular marker is a DNA fragment inserted after the 66120412 bp site on chromosome 5 corresponding to the International Pig Reference Genome Version 11.1; the nucleotide sequence of the DNA fragment is shown in SEQ ID NO:1. The structural variation molecular marker provided by this invention is significantly correlated with the trait of subcutaneous abdominal fat weight in pigs. By identifying this structural variation molecular marker, the trait of subcutaneous abdominal fat weight in pigs can be identified. Furthermore, by selecting individuals carrying the structural variation molecular marker during the breeding process, the subcutaneous abdominal fat weight of offspring pigs can be reduced, and the lean meat percentage of offspring pigs can be increased.

The invention belongs to the technical field of corn genetic breeding and molecular biology, and particularly relates to an InDel molecular marker closely linked with a major QTL locus qCE1 of a corn female ear clustered / monogenic character and application of the InDel molecular marker. The main effect QTL locus qCE1 is located on a first chromosome of a corn Zm-B73-REFERENCE-NAM-5. 0 reference genome, the marker interval is Chr.0123431188-23431982, and the main effect QTL locus qCE1 corresponds to a 2611-1817 bp region on the upstream of a Gt1 gene. The InDel molecular marker developed on the basis of different haplotypes of qCE1 has a remarkable effect in the aspect of identifying the corn female ear clustered / monogenic character and can be used for molecular marker-assisted breeding related to the corn female ear clustered / monogenic character, and the breeding process is accelerated.

This invention discloses a method for delivering a VIGS vector targeting the flower buds inside the bulb of Lycoris radiata, a method for verifying gene function, and their applications, belonging to the field of biotechnology. The method includes: making 4-6 holes around the root plate of a dormant Lycoris radiata bulb, and 2-4 holes in the central area of ​​the bulb base, reaching the meristematic region of the flower bud; immersing the treated bulb in VIGS infection solution up to the wounds on the root plate; and performing vacuum treatment to ensure effective penetration of the infection solution into the internal flower bud tissue. This invention successfully delivered a silencing vector targeting the LrP5CS1 gene to the flower bud, specifically reducing its expression and obtaining a stable early-flowering phenotype in Lycoris radiata, thus verifying the gene function. Therefore, this invention establishes an efficient delivery platform that can be used for rapid screening and verification of other key genes regulating the flowering period and ornamental traits of Lycoris radiata, providing a novel technical means for the functional study of genes related to the flowering phenotype of Lycoris radiata.

The invention provides an intelligent forest tree breeding method based on multi-modal data and a large model, and relates to the technical field of forest tree genetic breeding. The method comprises the steps of obtaining and integrating genotype data, phenotype data, multi-omics data and environment time sequence data of a target tree species, and performing preprocessing and feature fusion to form a multi-modal data set for analysis; on the basis of the multi-modal data set, key genes or gene modules for regulating and controlling target characters are mined by adopting a genotype-environment interaction algorithm and multi-omics coupling analysis; constructing a dynamic model fusing genotypes, environmental factors and time dimensions, and predicting breeding values of individuals or combinations on a whole genome level based on mined key genes or gene module information; and based on a prediction result, performing multi-objective optimization and intelligent screening at three levels of parents, combinations and offspring, and outputting a breeding decision scheme. According to the method, a complete, efficient and accurate forest tree intelligent breeding solution is formed.

The invention discloses an SNP molecular marker related to grass carp feed utilization rate and application thereof, and belongs to the technical field of aquatic animal molecular markers and aquatic genetic breeding. According to the invention, an SNP (Single Nucleotide Polymorphism) site obviously related to the feed utilization rate of the grass carp is screened out, the SNP molecular marker is positioned at the 51653399 site on the chromosome 2 of the grass carp, the polymorphic site of the SNP molecular marker is G / T, and when the genotype of the site is GG, the feed utilization rate of the grass carp is relatively high. The invention also discloses application of the SNP molecular marker, the amplification primer or the kit in breeding of grass carp with high feed utilization rate. The SNP molecular marker provided by the invention can be used for early breeding of grass carp with high feed utilization rate, greatly reduces the breeding workload, can significantly shorten the breeding time, accelerates the breeding process, improves the breeding efficiency, and reduces the breeding cost.

This invention provides molecular markers for SNPs (Special Nominal Nuclei) of the logarithmic rib trait in Meihuaxing pigs and their applications. The specific base sequences of the SNP molecular markers are shown in SEQ ID NO. 23-33. These molecular markers are mainly used for screening and / or identification of superior breeds. The advantages of this invention are that, in actual breeding, retaining individuals carrying superior genotypes helps improve breeding efficiency and selection accuracy. Simultaneously, it can increase the gene frequency of the superior economic trait of multiple ribs in the population, allowing for targeted improvement of the rib logarithmic trait, thereby enhancing the carcass performance and economic benefits of the entire population.

The application discloses a molecular marker closely linked to relative root length of wheat and application thereof, and belongs to the technical field of molecular marker breeding. The molecular marker is located at the 530103940th position of chromosome 7 in a Chinese spring wheat reference genome sequence RefSeq v2.1, when the base at the position is G, the relative root length of the wheat is relatively long; when the base at the position is C, the relative root length of the wheat is relatively short. By applying the molecular marker developed in the application, the relative root length of the wheat can be predicted, cost is saved, selection efficiency is greatly improved, the breeding process can be accelerated, and new possibilities are provided for efficient screening of excellent alleles of a TaHKT2; 7-D gene and cultivation of salt-tolerant and drought-resistant wheat varieties.