1282results about How to "Speed ​​up the breeding process" patented technology

The invention discloses a method for obtaining a temperature-sensitive sterile line by performing site-specific mutagenesis on P / TMS12-1 through a CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 system. The method comprises the following steps: cloning and controlling a Pei'ai 64S temperature-sensitive sterile major gene P / TMS12-1 fragment; designing a target sequence according to the P / TMS12-1 sequence; constructing a pU3-gRNA carrier of the target-containing sequence fragment; constructing a pCRISPR / Cas9 carrier; obtaining a positive transgenic seedling by utilizing the pCRISPR / Cas9 carrier containing the target sequence fragment; screening a mutant plant from the positive transgenic seeding; performing subculture planting on the mutant plant to obtain the temperature-sensitive sterile line without transgenic components. According to the method disclosed by the invention, the CRISPR / Cas9 system is utilized to completely inactivate P / TMS12-1 non-coding RNA, and the temperature-sensitive sterile line without transgenic components is artificially cultivated. The method disclosed by the invention has the advantages of being strong in purposiveness, small in genome damages and capable of avoiding transgenic interference.

The invention discloses a method for cultivating a low-cadmium-accumulation indica rice variety. The method includes the steps of cloning part of the CDS sequence of OsNramp5 of an indica rice acceptor material; using a CRISPR / Cas9 system to select a target sequence according to an exon sequence; building pCRISPR / Cas9 recombinant vectors; leading the pCRISPR / Cas9 recombinant vectors into rice calluses to obtain transgene seedlings; screening transgene positive plants; obtaining mutant plants; and conducting seed propagation of the mutant plants, and separating afunction mutants containing no transgenic ingredients from plant progenies. By means of the method, the cadmium absorption major gene OsNramp5 of indica rice is directionally knocked out through the CRISPR / Cas9 technology, and the indica rice material containing no transgenic ingredients, having no significantly variant comprehensive agronomic characters and achieving a low cadmium content in rice is directionally cultivated. The method has the advantages that targeting is efficient, the breeding period is short, cost is low and practicality is high.

The invention discloses a primer set for detecting functional genes of wheat on the basis of a KASP [competitive allele specific PCR (polymerase chain reaction)] technology and application of the set primer. The primer set comprises totally 14 groups of KASP primers. The KASP primers are respectively designed for the 14 functional genes of the wheat, and particular nucleotide sequences of the KASP primers are sequences 1-42 in sequence tables. The primer set and the application have the advantages that the functional genes of different wheat varieties can be quickly detected by the aid of the KASP primer set, methods for detecting the functional genes of the wheat are simple, convenient and speedy, and detection results are accurate and reliable; the primer set has an important theoretical significance and important economic value in assistant selection of the wheat varieties by the aid of molecular markers.

The invention discloses a core SNP sites combination maizeSNP384 for building of a maize DNA fingerprint database and molecular identification of varieties, and an application of the core SNP sites combination. The invention provides applications of 384 SNP sites in any one of the following conditions: (1) building of the maize DNA fingerprint database; (2) detecting of the authenticity of maize varieties; (3) genetic analysis of corn germplasm resources; and (4) molecular breeding of maize, wherein the physical positions of the 384 SNP sites are determined by comparison on the basis of a whole genome sequence of the maize variety B73; the version number of the whole genome sequence of the maize variety B73 is B73 RefGen V1; and the 384 SNP sites are MG001-MG384. An experiment proves that the 384 SNP sites can be applied to building of the maize variety DNA fingerprint database, identification of the variety authenticity, dividing of germplasm resource groups, and other related researches.

The invention discloses a construction method of superior strains of rapid-growing crassostrea gigas. The construction method is characterized by comprising the following steps: A. building a breeding base population, using the shell height and the total weight as a seed selection goal, and keeping and collecting parent shellfish according to high-selective intensity as the basic parent shellfish population; B. by adopting the population continuous progeny selection, selecting a certain number of gonadal matured individuals from the preceding generation of parent shellfish population to form a succeeding generation parent shellfish population, conducting artificial insemination by adopting a post-mortem method; at the pre-maturation period, keeping and collecting the individuals with high shellfish height and high total weight according to the high selective intensity as the parent shellfish for breeding; C, conducting the quantitative character detection and the genetic parameter estimation on the succeeding generation parent shellfish population in step B; D, repeating steps B and C while performing the genetic diversity analysis on three generations of the continuous progeny selection systems, and conducting the evaluation of the breeding effect. With the adoption of the construction method of the superior strains of the rapid-growing crassostrea gigas, the breeding progress is largely speeded up, the labor cost of breeding is reduced, the breeding effect is obvious, and the economic benefit of breeding the crassostrea gigas is improved.

The invention belongs to the field of fruit tree and forest tree breeding and specifically relates to a high-scion and head-changing grafting method of carya illinoensis. The high-scion and head-changing grafting method of carya illinoensis comprises the following steps of: before sprouting of carya illinoensis in spring, keeping the trunk by about 1 m away from the ground surface and sawing off the part thereabove to form a stumping cut, and then downwards cutting open at the (symmetrical) edges of the two sides of the stumping cut by using a grafting knife, thereby forming two cuts which are 3cm long and have width determined according to the thickness of the scion, and then grafting; next, bounding with a plastic film, binding the graft with a kraft bag, wherein the diameter of the kraft bag is determined by the thickness of the stock, sawdust is filled in the kraft bag to be higher than the scion, and the humidity of the sawdust is 70%; and then tightening the upper opening of the kraft bag; and finally, covering the kraft bag with a plastic bag; one month later, removing the plastic bag is removed and tearing off the kraft bag. With the method, the high-scion grafting survival rate of carya illinoensis is higher than 98%; therefore, the high-scion grafting survival rate of carya illinoensis is effectively realized.

The invention relates to the field of assistant breading in biotechnologies, in particular to an SSR marker linked with a pepper male sterility restoring gene Rf as well as an application of the SSR marker to selection of pepper male sterility breeding materials. The molecular marker closely linked with the pepper male sterility restoring gene is a pep43 marker or a pep20 marker. The development of the SSR molecular marker can accelerate the breeding process of the pepper male sterility, improve the breeding effect and provide a theoretical basis for classification and breeding of three systems of pepper CMS (cytoplasmic male sterility); an independently developed SSR primer and a conventional laboratory PCR (polymerase chain reaction) technology are adopted, and plants with restoring genes are selected through detection of pepper plants. The method is used for detecting genetic materials, is convenient and good in repeatability and can perform detection all the year round. According to a kit, the primer and related reagents for detection are integrally assembled in the kit, operations are convenient to implement, and detection results are stable and reliable.

The present invention provides a SNP marker associated with pig growth rate, and application thereof, wherein the SNP marker is located on the 201 bp position of the pig LXR[alpha] gene exon 5, and the growth rate of the pig with the base A at the position is significantly higher than the growth rate of the pig with the base C at the position. According to the present invention, the polymerase chain reaction (PCR) and the sequencing technology are adopted to screen the SNP marker associated with pig growth rate, the restriction fragment length polymorphism (RFLP) method is adopted to detect the genotype of the pig gene to be detected, and the breeding of the predominant variety of the pig with the rapid growth is performed according to the genotype so as to accelerate the pig breeding process.

The invention relates to the field of genetic engineering, in particular to protein ErNAC7 related to drought and salt resistance of plants and a coding gene and application thereof. The protein has an amino acid sequence shown as SEQ ID NO.1 and a gene sequence shown as SEQ ID NO.2. The protein related to the drought and salt resistance and the coding gene thereof have important theoretical and actual meanings for improving and enhancing the stress resistance of tobacco, improving yield, accelerating the breeding process of stress-resisting molecules and effectively saving water resources.

The invention relates to a technical method for high-efficient breeding of wheat, which comprises low-temperature vernalization, south breeding and generation adding, shuttle breeding, multi-point combined identification and the like. In particular, the technical method comprises the following steps of: treating the seeds obtained after the strong-winterness wheat blossoms for 15 to 20 days by hydrogen peroxide, washing the seeds by pure water, sprouting the seeds at the room temperature, putting the seedlings growing to 1 to 2 centimeters in an artificial weather room, performing the green-body vernalization of the seedlings for 45 and 50 days at a temperature of between 3 and 5 DEG C and a relative humidity of 70 percent and under a light intensity of 15001x; hardening the seedlings of the winter wheat undergoing the vernalization treatment at the normal temperature for 1 to 2 days, and transporting the seedlings to Yuanmou in Yunnan province; and preparing a rice seedling bed, watering and soaking the rice seedling bed, discharging visible water before transplanting, and transplanting the seedlings in mud at dusk. The disease resistance, stress resistance, adaptability and yieldstability selections of breeding materials are carried out by shuttle breeding and multi-point combined identification and the like. The technical method for breeding provides rich reserve of raw materials for the rapid improvement of the wheat variety, and also lays a sold foundation for demonstration and popularization of a new variety in large areas.

The invention discloses a method for improving the culturing efficiency of japonica rice anther. The method comprises the steps that firstly, culture medium mother solutions of the japonica rice anther are prepared according to formulas of the culture medium mother solutions of the japonica rice anther, then the various mother solutions are utilized to prepare an induction culture medium, a differentiation culture medium and a rooting culture medium all of which are used in different periods, and the culture media in the different periods are correspondingly applied to induction, green plantlet differentiation and rooting of calluses of the japonica rice anther respectively. By the adoption of the method, the percentage of seeding emergency of culturing of the japonica rice anther can be improved, the rice breeding progress is quickened, and the seed selection efficiency of good variety of rice is improved.

The invention relates to the field of gene engineering, in particular to a plant drought-resistance and salt-tolerance associated protein TaNAC, an encoding gene thereof and application thereof. The amino acid sequence of the protein is shown as SEQ ID NO.1 and the gene sequence is shown as SEQ ID NO.2. The plant drought-resistance and salt-tolerance associated protein and the encoding gene thereof of the invention has very important theoretical and practical significance for improving and enhancing the stress resistance of tobacco, increasing the yield, promoting the breeding process of stress resistant molecules and effectively saving water resources.

The invention belongs to the technical field of rape molecular marker preparation, and particularly relates to a molecular marker closely linked with the oil content QTL of cabbage type rape. The novel molecular marker OilA5 which is closely linked with the oil content QTL and can be used independently or / and used in combination is obtained through screening, and the nucleotide sequence of the molecular marker is shown in the sequence table SEQ ID NO:5 or 6. A primer pair OilA5-A and a primer pair OilA5-G for detecting the molecular marker OilA5 are constructed, and the nucleotide sequences of the primer pairs are shown in the sequence tables SEQ ID NO:1-4. The molecular marker can be applied to molecular marker auxiliary selection in cabbage type rape oil content character improvement and fine mapping and map-based cloning of oil content character sites.

The invention relates to detection primers for molecular markers in close linkage with a major QTL (Quantitative Trait Locus) of wheat ear length and an application of the detection primers. The detection primers for the molecular markers include an upstream primer, having a nucleotide sequence as shown in SEQ ID NO.1, of the molecular marker XWMC112 and a downstream primer, having a nucleotide sequence as shown in SEQ ID NO.2, of the molecular marker XWMC112, and an upstream primer, having a nucleotide sequence as shown in SEQ ID NO.3, of the molecular marker XCFD53 and a downstream primer, having a nucleotide sequence as shown in SEQ ID NO.4, of the molecular marker XCFD53. The molecular marker XWMC112 and the molecular marker XCFD53 are studied and found for the first time, and can be used for quickly determining whether a wheat variety or strain has the QTL of the ear length, and thus quickly screening out the wheat variety or strain having the QTL for increasing the ear length, and therefore, the breeding progress of a high-yield wheat variety can be accelerated.

The invention discloses a botanical seedling culturing machine. A control system and a water pump, an air compressor, an air conditioner and a pre-heater which are electrically connected with the control system are arranged in a primary unit segment and an external ventilator and an internal gas circulator which are electrically connected with the control system arranged in the primary unit segment are arranged in an auxiliary unit segment, at least one group of culturing chests of drawers (5) and sliding rails (18) for drawer; each group of culturing chests of drawers (5) are provided with operation shelves (13), working inter-layers (14) and movable meshes (16), and movable working discs (15); ventilation and fertilizer feed holes are arranged at the bottom of the movable working disc (15), a light strip module (28), a spraying pipeline and a nozzle (29), a high-pressure air pipe (30), and the reflux tank (32) of an irrigation tank (31) are arranged in the rippled grooves downwards of a plurality of notches on the working inter-layer (14). The invention is a plastic potted tray botanical seedling culturing machine without foam, polystyrene, and polypropylene capable of high-quality industrialized, intensified and entire-year seedling culturing seedling production of scale.

The invention provides a molecular marker, and particularly relates to cloning of a pig ECI1 gene segment and application as the molecular marker. A nucleotide sequence of the molecular marker is shown in SEQ ID NO.1; a C157-T157 base mutation is formed at the 157th bit, resulting in Bsm I-RFLP (restricted fragment length polymorphisms).

The invention provides a lysinecorn SSR (simple sequence repeat) molecular marker auxiliary selecting and breeding method. By combining a backcross transforming method with a molecular marking method, the method successfully constructs the lysinecorn isogenic gene systems QZ58, Q478 and QC72. After the SSR molecular marker auxiliary selecting and breeding method is used the mono-clones which contain the O2 gene can be detected by SSR molecular marker in the O2 gene after one generation is backcrossed, and the second generation can be backcrossed without selfing, so that the breeding is short in period, low in cost, and high in efficiency. Aiming at different breeding target requirements, the method is used for performing the gene type detection to the backcross offspring seedling leaves; and the method is not influenced by the environment conditions and combines with south propagating and generation adding, so that the target genes are transferred into a good material within a shorter time.

The invention discloses 384 SNP loci and application thereof in soybean variety resource identification. In the application disclosed by the invention, the physical locations of the 384 SNP loci are determined on the basis of whole genome sequence alignment of the soybean variety of Williams 82, and the version number of a whole genome sequence of the soybean variety of Williams 82 is Glyma.Wm82.al(Gmax1.01); the 384 SNP loci are SMK001-SMK384. An SNP chip based on the 384 SNP loci can be applied to identifying a soybean variety resource; the application of the combination of the 384 SNP loci can greatly improve the detection accuracy, shorten the detection time and improve the detection level, which provides a strong technical support for soybean variety popularization and seed market testing, and accelerates the seed selection process of an excellent new soybean variety.

The invention discloses a method for building a high-efficiency regeneration system of superior corn self-bred line agriculture line 531, belonging to the field of plant genetic engineering and transgenosis breeding. The invention takes an agriculture line 531 rataria as an explant, induces in a callus induction medium and produces an II-type embryonic callus; the II-type embryonic callus is subjected to embryoid induction under light in an embryoid induction medium to produce a green embryoid; then, the green embryoid is transported to a regeneration medium and is cultured into a regeneration plant under light; root induction is carried out in a rooting medium, and acclimatization is carried out in Hogland nutrient solution to ensure that a new thick root grows on the root of the regeneration plant; the root is transplanted to nutritional soil for rejuvenation culture; and finally, the root is transplanted to a land for growing field crops to normally grow and seed. The regeneration technology is suitable for the superior corn self-bred line agriculture line 531 with high application value, can ensure that the superior quality of agriculture line 531 corn can be inherited in the corn transgenosis breeding process, and has an important meaning for functional genome group research.

The invention discloses a cutting propagation method of eustoma grandiflorum. The cutting propagation method includes: day temperature of a female parent cultivation greenhouse is kept at 20-30 DEG C, night temperature is kept at 13-18 DEG C, relative air humidity during the day is kept to be 50-75%, and relative air humidity during the night is kept to be 70-85%; and sunshine time in a year is less than 13 hours. When a lateral bud of a female parent plant has eight leaves and one heart, a bigslip is cut out, cutting is performed on the bigslip after the bigslip is dipped in rooting powder, shading coefficient of an outer shading net of a cutting greenhouse is 30%, inner relative air humidity is kept to be 80-95% through high pressure mist, temperature is at 20-25 DEG C, CO2 concentration in the cutting greenhouse is kept to be 600-800ppm through CO2 fertilization, and a cutting seedling is transplanted to a flower cutting production greenhouse after the cutting seedling takes root. The cutting propagation method of eustoma grandiflorum reduces root disease rate and leaf disease rate substantially, shortens seed breeding stage, and promotes the eustoma grandiflorum pigslip to take root. Rooting time of eustoma grandiflorum is 23-25 days, rooting rate of eustoma grandiflorum is above 92%, and planting percent of eustoma grandiflorum is above 87%. The cutting propagation method of eustoma grandiflorum is suitable for commercial and large-scale propagation of eustoma grandiflorum.

The invention relates to a breeding and cultivating method for a plant, in particular relates to a breeding and cultivating method for loose purple cauliflowers, and belongs to the technical field of plant cultivation. The breeding and cultivating method comprises the steps of hybridizing a loose purple cauliflower breeding material with a semi-compact purple cauliflower breeding material to obtain seeds F1, and selfing single strains with high comprehensive agronomic characters after the seeds F1 are sowed to obtain seeds F2; performing microspore culture on single loose purple cauliflowers through flower buds suitable for the microspore culture from the mononuclear edging stage to the double-core early stage to grow a large number of normal regenerated plants, selfing excellent DH plants of the loose purple cauliflowers to obtain a loose purple cauliflower excellent DH strain 03-12 serving as a recurrent parent which is hybridized with a cauliflower cytoplasmic male sterility source material CMS05-6, performing five generations of backcross transformation and selection to form a cytoplasmic male sterile line CMS08-2, and hybridizing the DH strain serving as a parent with the excellent cytoplasmic male sterile line CMS08-2.

The invention discloses a rice ALS (acetolactate synthase) mutant type protein, a mutant type gene and applications of the rice ALS mutant type protein and the mutant type gene. The amino acid sequence of the ALS mutant type protein has following mutation: the 628-poisiton amino acid corresponding to the amino acid sequence of rice ALS has the mutation. The invention further discloses a breeding method of creating herbicide-resistant rice by gene editing. The CRISPR / Cas9 gene editing technology is used for editing the ALS gene for the first time, a T-DNA-knockout new material with the stably inherited herbicide resistant characteristic can be obtained in the T2 generation through offspring screening, and basic agronomic traits of the new material have no obvious change. Compared with breeding based on chemical mutagenesis, hybrid transform breeding and the like, the orderly improved molecular breeding technology based on gene editing has the advantages of being rapid, accurate, efficient and the like, and by means of combination with gene function marked genotype selection, breeding efficiency can be greatly increased and breeding progress is substantially accelerated.

The invention provides a mutant gene of a rice starch branching enzyme SBE3 gene. The mutant gene has a basic group mutation of T--C in a position corresponding to the 105th position of the 16th exon of the rice starch branching enzyme SBE3 gene. The invention further provides a method for screening rice variety with high resistant starch content. The basic group mutation of the mutant gene can be served as a molecular marker to effectively detect Jiangtangdao 1 with high resistant starch content and derived varieties (lines) of Jiangtangdao 1, so that the selection efficiency of the new rice variety with high resistant starch content is greatly improved and the rice variety with high resistant starch content is obtained.

The invention discloses a tissue-culture rapid propagation method for pyrus betulaefolia bunge. The tissue-culture rapid propagation method for the pyrus betulaefolia bunge comprises disinfecting on explants, inducing buds, propagating test tube seedlings, inducing calluses, inducing rooting and transplanting exercising seedlings. During the process of inducing rooting, the calluses are firstly induced and then the rooting is induced; obtained rooting test tube seedlings are performed seedling exercising in a triangular flask, then transferred to perlite to domesticate the exercising seedling and finally transplanted. Compared with the traditional method, the tissue-culture rapid propagation method for the pyrus betulaefolia bunge has the advantages of being high in rooting rate and survival rate of transplanting, high in propagation rate, low in cost, convenient to popularize, good in genetic stability and suitable for the pyrus betulaefolia bunge.

The invention discloses a culture method for triploid grape culture by embryo rescue and early identification of ploidy. The invention aims to cultivate triploid grape by embryo rescue and overcome the problem of seed abortion of hybrid embryo between diploid and tetraploid grape cultivars in conventional breeding. After a hybrid seed is obtained, chromosome count is performed by the combination of flow cytometry and wall degradation hypotonic method to identify the ploidy of the hybrid progeny. The invention increases the breeding efficiency of triploid grape, accelerates the breed selection of triploid grape variety and provides a new approach to the culture of seedless grape variety.

The invention provides a herbicide-resistant rape directive breeding method based on acetolactate synthase (ALS) target esterase, which belongs to a plant trait breeding method. The method comprises the following steps of selecting materials needed by production of rape or breeding of the rape, processing seeds with ethylmethane sulfonate (EMS), mutagenizing the seeds to be isolated and propagated to a M2 generation, and directionally screening herbicide-resistant mutant characters in a large group under the selection pressure of the herbicide adopting the ALS as the target esterase. In order to increase the probability for acquiring the mutant characters, the directive screening can be continuously conducted in multiple years, or conducted in multiple places in the same year or conducted in multiple places in multiple years until the needed mutant material is screened out.

The present invention relates to a high-efficient breeding technique of Eurasian raisin grape. After the raisin grape has flowered for 30-50d, the young fruit of the grape is picked. The ovule is taken out in a sterilized state. The embryo is inoculated to an MS culture medium containing different levels of plant growth regulators of gibberellic acid (GA3), indoleacetic acid (IAA) and 6-benzylaminopurine (6-BA) for executing embryo rescue. The average embryo development rate using the breeding technique of the invention is increased from 8.3-11.9% of a patient disclosed in the background art to 84%. The germination rate obtains 75.33% and the seedling rate obtains 74.17%. Furthermore the embryo rescue technique of the raisin grape obtained through the technique of the invention has the advantages of increased seedling rate of offspring, easier obtainment of more filial generation, increased parent selection range of raisin grape cross breeding, accelerated breeding process of new species of the raisin grape and increased breeding efficiency of the raisin grape through increasing the embryo development rate and the germination rate of the raisin grape.

The invention provides a method for screening specific molecular markers of rice fertility restorer genes, which is characterized by applying rice germ plasm resources carrying and not carrying fertility restorer genes and screening the molecular markers, the sterile lines and (or) the restorer lines of which are in specific banding patterns, from the molecular markers closely linked with the rice fertility restorer genes Rf3 and Rf4. With the markers adopted to carry out auxiliary selection on the molecular markers of rice fertility restorer genes, the breeding efficiency of the sterile lines and the restorer lines of indica type rice can be improved.

The invention discloses a screening method for breeding Streptomyces roseosporus strains producing Daptomycin and the obtained strains. The screening method comprises the following steps of: 1) coating bacteria liquid of wild Streptomyces roseosporus or mutant strains thereof subjected to mutagenesis on a solid panel containing streptomycin and / or Daptomycin, and culturing to obtain streptomycin and / or Daptomycin tolerant Streptomyces roseosporus; and 2) respectively selecting a single colony of the streptomycin and / or Daptomycin tolerant Streptomyces roseosporus for shake flask fermentation, detecting the Daptomycin content in fermented materials, and selecting strains with the Daptomycin content higher than that of starting strains. The screening method has the advantages of directivity, intuition, simpleness, quickness, high efficiency, great reduction of workload, and acceleration of strain breeding process. The Daptomycin fermentation unit of the screened Streptomyces roseosporus strains is obviously improved, which can be improved by more than 5 times to the maximum.

The invention discloses a molecular-marker-assisted breeding method of a maize haploid inducing line and a special primer thereof. The primer is composed of a DNA (deoxyribonucleic acid) molecule disclosed as SEQ ID NO.1 and a DNA molecule disclosed as SEQ ID NO.2. The test proves that the molecular-marker-assisted breeding method of a maize haploid inducing line can directly identify a single plant containing two main effect QTLs (quantitative trait loci) qHI-1 and qHI-8, eliminate the other genotypes and quickly obtain the two-locus homozygosis plant line, thereby saving abundant unnecessary examination and haploid selection work, obviously enhancing the haploid inducing line breeding efficiency, accelerating the haploid inducing line breeding progress and increasing the breeding probability of favorable inducing lines.