52results about How to "Speed ​​up the breeding process" patented technology

The invention relates to a molecular marker which is located at the 47023718th basic group from the 5tail end on a No.16 chromosome of a pig reference genome Sscrofa11.1 and is related to a pig internal farrowing litter weight standard deviation. The invention describes a primer pair and a kit for detecting the molecular marker, and application of the primer pair and the kit in identifying the size of the standard deviation of the litter weight in the pig, screening pig varieties or genetic breeding of the pig. The pig internal litter weight standard deviation of the AA genotype individual is 2.79 kg, the pig internal litter weight standard deviation of the GG genotype individual is 4.38 kg, and the AA genotype individual is obviously smaller than the GG type individual, namely, the pig resilience of the AA genotype individual is higher than that of the GG individual. The AA type individuals are selected, and the pigs with small standard deviation of litter weight and high restoring force in the pigs can be obtained. According to the method, the problem of determining the pig resilience in actual production is effectively solved, the breeding cost is reduced, the pig internal litter weight standard deviation is effectively reduced, so that the pig resilience is improved, the method is high in accuracy and low in detection cost, automatic detection can be realized, and the method has very high practical application value in the aspect of pig breeding.

The invention relates to the technical field of rice molecule-assisted breeding, in particular to a molecular marker of major QTL qCST3 for regulating and controlling rice salt tolerance and application of the molecular marker, major QTL is located on a rice chromosome 3 and named as qCST3, and the physical position is 30.48 Mb to 32.90 Mb; the SNP marker closely linked with the SNP marker is located on the 33887916 site of the chromosome 3, and when the position is C, the rice is indicated to be salt-tolerant rice; and when T is equal to T, indicating that the rice is salt-sensitive rice. The novel major QTL and the linked molecular marker thereof provided by the invention can be used for quickly judging the salt tolerance level of rice, so that the rice variety screening and breeding process is accelerated.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to the tolerance character of white spot syndrome virus (WSSV) of procambarus clarkia, the molecular marker is located in the third intron region of a TRIM gene, is specifically located at 585bp of a DNA (Deoxyribose Nucleic Acid) sequence as shown in SEQ ID NO.1, and is Tgt; the T / C heterozygous genotype is obviously related to the WSSV tolerance character of the procambarus clarkia. The invention provides a favorable marker for breeding of disease-resistant molecules of procambarus clarkii, and can accelerate the breeding process of disease-resistant varieties of procambarus clarkii.

The invention relates to the field of molecular biology and genetic breeding, and provides application of an SNP (Single Nucleotide Polymorphism) marker related to a body height character. The SNP marker is located at the 136651710 bp position of the No.3 chromosome of sheep, and is Cgt; and T base mutation (rs403130818) is carried out. Experiments show that the SNP marker developed by the invention has significant influence on the sheep body height character (Plt; 0.05), and the allele T has more excellent body height character, so that the SNP molecular marker in the MCRS1 gene can be applied to molecular marker breeding of the sheep body height character. The molecular marker provided by the invention is not limited by the age, sex and the like of the sheep, can be used for breeding excellent body height sheep varieties, and even can be accurately screened when the sheep are newly born, so that the breeding process of the excellent body height sheep varieties is greatly accelerated.

The invention discloses a method for cultivating a heat-resistant broad-spectrum durable rice blast-resistant rice breeding material based on polygene polymerization. According to the method, a high-heat-resistance and high-dominance indica-japonica hybrid indica rice restorer line is selected as a recurrent parent, a breeding intermediate material for polymerizing complementary broad-spectrum rice blast resistance genes Pigm and Pi-jx is used as a donor parent, and polymerization of the heat-resistance and broad-spectrum lasting rice blast resistance genes Pigm and Pi-jx is realized through a method of combining molecular breeding with conventional breeding. Meanwhile, by selecting representative strains to carry out artificial inoculation identification of seedling blast and panicle blast, natural induction identification of a rice blast retransmission area and heat resistance identification, and by combining main agronomic trait evaluation in the whole growth period, a heat-resistant broad-spectrum durable rice blast-resistant breeding material is obtained. The breeding material bred by using the method disclosed by the invention has relatively strong heat resistance and broad-spectrum and lasting rice blast resistance, and the breeding process of heat-resistant and rice blast-resistant rice varieties is accelerated.

This invention relates to the fields of molecular markers and animal genetic breeding technology, specifically to a molecular marker located on chromosome 4 of pigs that is associated with body size and carcass traits, and its application. Using the F7 generation of a chimeric family as the research object, this invention employs whole-genome resequencing and GWAS analysis to study and identify the molecular markers located on chromosome 4 of pigs associated with body size and carcass traits. By optimizing the dominant alleles of these molecular markers, the frequency of dominant alleles can be increased generation by generation, enabling rapid and accurate selection of body size and / or carcass traits, accelerating the progress of pig genetic improvement, and thus effectively improving the economic benefits of pig breeding.

The invention relates to the technical field of biology, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker related to soybean mosaic virus resistance, an amplification primer and application of the SNP molecular marker in soybean breeding. The SNP molecular marker is located at the 910489bp site of a No.7 chromosome of a soybean genome, and the polymorphism of the SNP molecular marker is C / A. The invention provides application of the SNP molecular marker related to the soybean mosaic virus resistance in identification or auxiliary identification of the soybean mosaic virus resistance. By providing the accurate, simple, convenient and low-cost SNP molecular marker and the detection method thereof, early-stage, rapid and efficient molecular-assisted selection of soybean mosaic virus resistance characters is realized, so that the breeding process of a new variety of disease-resistant soybeans can be remarkably accelerated, and the SNP molecular marker has important breeding application value.

The invention discloses a molecular marker closely linked with a major QTL (Quantitative Trait Loci) of the row number of corncobs and application of the molecular marker. A molecular marker closely linked with a major QTL locus qPsa1 with significantly increased ear row number in the process of domesticating teosinte into corn is located at the 270911250th site of a first chromosome of a corn V4 genome, and the genotype of the molecular marker is CC, TT or CT. The molecular marker PSA1KASP can be used for selecting excellent allelic variation of qPsa1 in'redomestication 'or'de novo domestication' corn new varieties or strains, so that the workload of breeding and screening can be greatly reduced, the breeding period is shortened, and the breeding process of corn with a large number of rows per ear is accelerated.

The invention provides a molecular marker, a detection reagent and a method for evaluating growth traits and / or egg laying interval traits of meat pigeons. The SNP locus and the haplotype molecular marker which are remarkably related to the body size character, the body weight character and / or the egg laying interval character of the meat pigeon are screened from the first intron of the ASIP gene of the meat pigeon, and can be applied to early auxiliary selection of meat pigeon breeding. The invention further provides a detection reagent and a detection method for detecting the SNP loci and the haplotype molecular markers, the body size character, the body weight character and / or the egg laying interval character of the meat pigeons can be quickly, effectively and accurately predicted, so that the production cost is saved, the breeding process is accelerated, and the detection reagent and the detection method have high economic application value and scientific research value.

The present application relates to the technical field of plant genetic engineering, and in particular to a circular RNA PacircCNGC of Polygonatum cyrtonema and application thereof.The cDNA nucleotide sequence of the circular RNA PacircCNGC is shown in SEQ ID NO.1 or SEQ ID NO.2.The present application finds the role of the circular RNA PacircCNGC of Polygonatum cyrtonema in plant drought resistance and salt resistance, provides a theoretical basis and gene resource for resistance breeding of Polygonatum cyrtonema, provides a powerful tool for transgenic research of Polygonatum cyrtonema, provides a valuable circular RNA for molecular breeding of Polygonatum cyrtonema, and has important significance for revealing the molecular mechanism of bamboo resistance, breaking through the limitations of conventional breeding, and accelerating the breeding process of bamboo.

The application of broccoli genes BoSULTR1;1 and BoSULTR1;2 in improving plant stress resistance belongs to the technical field of plant genetic engineering. In order to solve the technical problem of how to improve the stress resistance of broccoli plants, the application provides the broccoli genes BoSULTR1;1 and BoSULTR1;2, and the application uses an expression vector or a recombinant bacterium overexpressing BoSULTR1;1 and BoSULTR1;2 genes to prepare a transgenic broccoli plant. The stress resistance related detection results prove that the BoSULTR1;1 and BoSULTR1;2 genes have the ability to improve the resistance of the transgenic broccoli plant to cotton bollworm, pathogenic bacteria and drought. The transgenic material obtained by the application has important theoretical significance and practical value for accelerating the breeding process of stress-resistant plants and improving the breeding efficiency.

The invention discloses an automatic drying device for peanut breeding, and relates to the technical field of agricultural machinery, the automatic drying device comprises a box body, the interior of the box body is of a cavity structure, and the top of the box body is connected with a top cover through a hinge; the drying bin is arranged in the box body, a bin door on the front side of the drying bin can be opened, a sliding groove is formed in the bottom of the front side of the drying bin, a telescopic rod is arranged in the sliding groove in a sliding mode, the rear side of the drying bin is in lap joint with the platform, and a certain margin exists between the rear side of the drying bin and the platform on the left side; a transmission rod is arranged below the platform on the left side of the drying bin, one end of the transmission rod is connected with a motor, the other end of the transmission rod is connected with a first gear, the middle of the transmission rod is further sleeved with a cam, and the cam collides with the drying bin while rotating along with the transmission rod so that the drying bin can shake left and right. According to the automatic drying device, powerful support is provided for peanut breeding work through the efficient and accurate drying effect and the automatic operation process.

The invention discloses an SNP (Single Nucleotide Polymorphism) site for inheriting and improving meat quality traits of long-tailed chicken on Dam, application of the SNP site, a primer for detection, a method for detecting the meat quality traits of long-tailed chicken on Dam by utilizing the site, and a genetic breeding method. The SNP molecular marker is a variation site g.96619544Cgt of a 12th exon of a GBE1 gene on a chicken chromosome 1; the gene is T (rs313212342), the reference genome is GRCg6a, and the transcript is ENSGALT00000091071. The leg muscle fatty acid content of the CT genotype of the site is obviously higher than that of the TT genotype (Plt; 0.05), especially in key indexes of polyunsaturated fatty acids (PUFAs) such as alpha-linolenic acid and the like, and the CT genotype is a dominant genotype of indexes such as carcass weight, abdominal fat weight, leg muscle weight, full eviscerating weight and the like.

This invention discloses a KASP molecular marker related to the dilution value of wheat starch gelatinization characteristics and its application, relating to the field of molecular biology. The SNP site corresponds to the physical location 244495255 bp on chromosome 7A of the Chinese spring wheat genome (IWGSC RefSeq v1.0), named qRVA-Peak7A.1 SNP, corresponding to the 101st base from the 5' end of the sequence shown in SEQ ID NO: 4. When this site is homozygous for AA, the corresponding genotype is A; when this site is homozygous for GG, the corresponding genotype is B. The application is to screen or assist in screening wheat with larger dilution values ​​under normal water and fertilizer and / or drought stress and / or low nitrogen stress conditions. The wheat dilution value is such that wheat homozygous for genotype B is greater than or can be greater than wheat homozygous for genotype A. The KASP marker developed in this invention is related to the dilution value of wheat starch gelatinization characteristics and can be used to assist in the selection of wheat germplasm with different starch gelatinization characteristic dilution values ​​under normal water and fertilizer and / or drought stress and / or low nitrogen stress conditions.

The invention belongs to the field of fish genetic breeding, and particularly relates to a method for preparing sterile triploid fish with a tyr gene knocked out, which comprises the following steps: S1, knocking out wild type female diploid red crucian carp and male autotetraploid crucian carp high mutants through the tyr gene, and screening out the female diploid red crucian carp high mutant and the male autotetraploid crucian carp high mutant; and S2, hybridizing the female diploid red crucian carp high mutant and the male autotetraploid crucian carp high mutant to prepare the tyr gene knockout sterile triploid crucian carp homozygous mutant on a large scale. By utilizing the method, the triploid fishes with the accurately improved characters are prepared on a large scale, and the problem of biological safety is solved due to the natural sterility characteristics of the triploid fishes, so that the method has important practical value and ecological significance for genetic breeding and sustainable development in aquaculture.

The invention discloses an SNP molecular marker related to grass carp feed utilization rate and application thereof, and belongs to the technical field of aquatic animal molecular markers and aquatic genetic breeding. According to the invention, an SNP (Single Nucleotide Polymorphism) site obviously related to the feed utilization rate of the grass carp is screened out, the SNP molecular marker is positioned at the 51653399 site on the chromosome 2 of the grass carp, the polymorphic site of the SNP molecular marker is G / T, and when the genotype of the site is GG, the feed utilization rate of the grass carp is relatively high. The invention also discloses application of the SNP molecular marker, the amplification primer or the kit in breeding of grass carp with high feed utilization rate. The SNP molecular marker provided by the invention can be used for early breeding of grass carp with high feed utilization rate, greatly reduces the breeding workload, can significantly shorten the breeding time, accelerates the breeding process, improves the breeding efficiency, and reduces the breeding cost.

The invention relates to the technical field of biology, in particular to a KASP molecular marker for tobacco drought resistance characters and application of the KASP molecular marker. The molecular marker c22-1 and the molecular marker c22-4 which are closely linked with the tobacco drought resistance character are finally obtained by carrying out a first round of screening on the tobacco drought resistance character gene based on sequencing data and then carrying out a second round of large-scale screening on the tobacco drought resistance character gene by utilizing a KASP technology. The molecular marker is used for breeding the drought-resistant tobacco variety, the detection result stability is good, the repeatability is high, the detection result consistency is good, a more accurate scientific basis can be provided for subsequent gene function research and breeding work, and the breeding process of the drought-resistant tobacco variety is accelerated.

The application discloses a haplotype related to cotton fiber strength and a functional KASP molecular marker and detection application thereof, and relates to the technical field of plant molecular breeding and genetic markers. The haplotype is located in a 5,980,849-5,982,917 bp segment of a cotton reference genome D08 chromosome, is composed of 7 SNP sites, and can be divided into two types of Hap1 and Hap2. The KASP molecular marker is located at 5,982,917 bp of the cotton reference genome D08 chromosome, and the base is A / T. The haplotype represented by the molecular marker is significantly related to the cotton fiber strength, the KASP detection primer developed based on the haplotype has the advantages of accurate detection, simple operation, high throughput and low cost, and can be used for screening of early-stage materials in cotton breeding, significantly improves the selection efficiency of the fiber strength, and speeds up the breeding process of new cotton varieties with high quality.

The invention discloses a Camel hoof valve gene ZbBCAT2 and application thereof to improvement of salt tolerance of plants. The CDS sequence of the Camel hoof valve ZbBCAT2 gene is as shown in SEQ ID NO.1, and the coded amino acid sequence is as shown in SEQ ID NO.2. The invention further discloses a preparation method of the Camel hoof valve gene ZbBCAT2. The invention also discloses an application of the Camel hoof valve gene ZbBCAT2 in improving the salt tolerance of plants. The overexpression of the Camel hoof valve ZbBCAT2 gene plays a negative regulation role in the salt tolerance. The Camel hoof valve ZbBCAT2 gene provided by the invention can be used as a candidate gene for breeding the salt-resistant new variety of the plant, and has important significance for cultivating the salt-resistant new variety of the plant by adopting a genetic engineering method, accelerating the breeding process and reducing the breeding workload.

This invention relates to the fields of molecular biology and genetic breeding, and particularly to the dCAPS marker associated with the length of adventitious roots in wheat under waterlogging stress and its application. This dCAPS molecular marker is based on the 234,677,747th base of wheat chromosome 6B; the SNP base difference is either C or T. This molecular marker is accurate and efficient to detect, convenient and stable to amplify, and can be used for marker-assisted selection to improve the efficiency and accuracy of identifying the waterlogging stress resistance of wheat plants.

The invention belongs to the technical field of molecular biology, and particularly relates to a wheat stripe rust resistance gene TaPBL16-1B functional molecular marker and application thereof. The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1, and the molecular marker is located at the 226361575 to 226360891 sites of a wheat 1B chromosome and directly indicates a wheat stripe rust resistance gene TaPBL16-1B. Wheat containing the molecular marker has the stripe rust resistance character, and wheat without the molecular marker does not have the stripe rust resistance character. Whether the stripe rust resistant wheat variety is resistant to stripe rust or not can be accurately and efficiently identified by utilizing the molecular marker and the amplification primer thereof, and the molecular marker can be applied to wheat molecular marker-assisted breeding, so that the breeding selection efficiency is greatly improved.

This invention discloses a method for promoting somatic embryogenesis and maturation in Cryptomeria japonica, belonging to the field of somatic embryogenesis technology. The method involves using immature seed embryos as explants to induce embryogenic callus; transferring the proliferated embryogenic callus to a somatic embryogenesis induction medium for further culture to obtain somatic embryos; transferring the somatic embryos to a somatic embryogenesis maturation medium for dark culture to obtain mature cotyledonary embryos; transferring the mature cotyledonary embryos to a somatic embryogenesis basal medium for light culture to obtain somatic embryonic seedlings; and hardening off the seedlings before transplanting to obtain regenerated plants. The results of this application show that embryogenic callus can be stably obtained from different families, with high somatic embryogenesis maturation capacity and rate, enabling the cultivation of a large number of high-quality seedlings with stable genetic traits in a short time; avoiding the phenotypic segregation problem caused by gene recombination during seed propagation, and ensuring that offspring plants inherit the superior characteristics of the parents.

This invention belongs to the field of plant genetic engineering, specifically relating to an isolated nucleic acid molecule encoding the Sapindus mukorossi SmHD-ZIP34 protein and its application. The isolated nucleic acid molecule has a nucleotide sequence as shown in SEQ ID NO.1, or encodes an amino acid sequence as shown in SEQ ID NO.2. This invention reveals for the first time the dual function of this isolated nucleic acid molecule in simultaneously regulating flowering time and sex differentiation in plants. Experiments have shown that overexpression of this nucleic acid molecule in Arabidopsis thaliana significantly promotes flowering and pistil elongation, and inhibits stamen development by downregulating the core pathway of stamen development, leading to a reduction in the number of stamens and pollen abortion. The nucleic acid molecule resource provided by this invention offers an effective solution to the problems of long breeding cycles and low fruit set rates due to male flower dominance in economic forest trees such as Sapindus mukorossi, and can be used to cultivate high-yielding new germplasm with early flowering, fewer males, and more females.

The application discloses a parent-offspring identification primer group and method of Prunus. The parent-offspring identification primer group of Prunus disclosed by the application is composed of 30 single-stranded DNAs shown in sequence 1 to sequence 30 in a sequence list. The primer group and method of the application can identify the genetic relationship of Prunus armeniaca, Prunus dulcis and their offspring, establish a parent-offspring identification system, and are simple to operate. The primer group and method of the application can quickly obtain genetic information of Prunus parents and offspring and determine the source of the offspring, can early determine and retain hybrid offspring between Prunus armeniaca and Prunus dulcis at the seedling stage, which is beneficial to quickly determine the true outcrossing seedling at the seedling stage, thereby removing hybrid offspring caused by natural pollination and eliminating the hybrid offspring at the seedling stage, so as to save land resources, reduce the identification cost in the later period, speed up the breeding process, and also provide a reference for parent-offspring identification of other fruit plants.

The application provides a screening method of a dairy cow oocyte donor, comprising the following steps: investigating the correlation between the body condition score, age, parity, postpartum days and milk yield of the donor and the development potential of the dairy cow oocyte; determining the body condition score, age, parity and postpartum days as the screening indexes of the donor; and screening the donor based on the screening criteria of the screening indexes. The screening method can screen the donor with high oocyte development potential, which will help to improve the selection and management strategy of the live oocyte donor cow, and provide a theoretical basis and practical guidance for improving the reproductive efficiency of the dairy cow, promoting the breeding process and improving the economic benefit of the industry.

The invention discloses a diagnostic KASP marker for detecting a wheat stalk rust resistance gene Sr43 and application of the diagnostic KASP marker, and belongs to the technical field of wheat molecular breeding. By utilizing the diagnostic KASP marker disclosed by the invention, the existence condition of the Sr43 gene can be known in advance, the resistance of wheat to the Puccinia graminicola can be predicted, genetic typing is carried out according to the fluorescent type detected by the marker, a strain carrying the wheat Puccinia graminicola resistance gene Sr43 is accurately screened out for wheat breeding, and the molecular marker can directly serve production practice and has a wide application prospect. The breeding efficiency of the wheat Puccinia stelleriana Sr43 gene is improved, the breeding process is accelerated, and the application of the gene in wheat molecular breeding is promoted.

This invention relates to the field of poultry breeding technology, specifically to a breeding method for a high-breeding, fast-growing, dwarf, slow-feathering Guangxi Ma chicken. The method includes: constructing a molecular detection system for the sex-linked dwarf gene GHR and the recessive white-feathered RWC gene; hybridizing Guangxi Ma chicken breeding hens with dwarf, recessive white-feathered breeding roosters to obtain a hybrid F1 generation; self-pollinating the F1 generation to produce the F2 generation; using phenotypic selection combined with molecular detection technology to screen out dwarf, slow-feathering Guangxi Ma chicken individuals in the F2 generation; subsequently selecting breeding roosters and hens to form a core group of dwarf, slow-feathering Guangxi Ma chickens; establishing a Generation 0 family; and through successive generations of selection to develop a new high-breeding, fast-growing, dwarf, slow-feathering Guangxi Ma chicken strain. The dwarf, slow-feathering Guangxi Ma chicken strain bred by this invention inherits the dwarf characteristics of dwarf, recessive white-feathered chickens, has a lower feed intake during the laying period compared to Guangxi Ma chickens, and increases egg production, fertilization rate, and egg quality rate, effectively reducing breeding costs; simultaneously, the use of gene detection technology improves the accuracy and efficiency of breeding.

The invention discloses application of an InDel molecular marker located on a pig chromosome 16 and related to nipple number heterosis, the locus of the InDel molecular marker is rs793470896, and the InDel molecular marker corresponds to GGAGGCACgt at the 74359589 bp position on the chromosome 16 of an international pig reference genome version 11.1; the gene type of the InDel molecular marker is GGAGGCAC / GGAGGCAC, GGAGGCAC / G or G / G. The gene type of the InDel molecular marker is GGAGGCAC / GGAGGCAC. The InDel molecular marker provided by the invention is used for marker-assisted selective breeding, pigs with the genotype of GGAGGCAC / GGAGGCAC in a group are gradually eliminated, and the nipple number of bred sows can be remarkably increased, so that the piglet breeding and lactation capabilities of the sows can be remarkably improved, the survival rate of piglets can be increased, and the production income can be increased.

The invention provides an SNP marker related to an upland cotton plant type and application, and relates to the technical field of molecular marker assisted breeding, the SNP marker is located at the 14762561th basic group of an 18th chromosome D05 of an upland cotton genome, in an upland cotton plant with a compact plant type and a tower type (shorter fruit node), the nucleotide type of the SNP marker is T, and the nucleotide type of the SNP marker is T; in upland cotton plants with loose and cylindrical plant types (long fruit nodes), the nucleotide type of the SNP marker is G. By detecting the genotype of the SNP marker, the plant type (fruit knot length) of the to-be-detected upland cotton germplasm can be quickly and accurately judged, so that the variety with short fruit knot and compact plant type can be quickly and reliably identified on the gene level without being influenced by the environment and the growth and development period, and the breeding accuracy and efficiency of the variety with short fruit knot and compact plant type are improved; the SNP variation site provided by the invention can be used as a target of genetic engineering breeding, and a rapid and effective way is provided for genetic improvement of upland cotton fruit node length traits.

This invention discloses a rice blast resistance gene. Pilx SNP molecular markers, primer sets, and applications, wherein the SNP molecular markers are... Pilx The SNP molecular marker Pilx The polymorphic site is located on rice chromosome 12. Pilx The 18066332nd base of the gene is either T or C. This invention relates to a rice blast resistance gene. Pilx SNP molecular markers were used, and PARMS technology was applied to the reaction of rice materials. Pilx Genetic testing can rapidly and accurately detect rice blast resistance genes in different germplasm resources such as indica and japonica rice. Pilx .