12results about How to "Speed ​​up the breeding process" patented technology

This invention relates to the fields of molecular markers and animal genetic breeding technology, specifically to a molecular marker located on chromosome 4 of pigs that is associated with body size and carcass traits, and its application. Using the F7 generation of a chimeric family as the research object, this invention employs whole-genome resequencing and GWAS analysis to study and identify the molecular markers located on chromosome 4 of pigs associated with body size and carcass traits. By optimizing the dominant alleles of these molecular markers, the frequency of dominant alleles can be increased generation by generation, enabling rapid and accurate selection of body size and / or carcass traits, accelerating the progress of pig genetic improvement, and thus effectively improving the economic benefits of pig breeding.

This invention relates to the fields of molecular biology and genetic breeding, and particularly to the dCAPS marker associated with the length of adventitious roots in wheat under waterlogging stress and its application. This dCAPS molecular marker is based on the 234,677,747th base of wheat chromosome 6B; the SNP base difference is either C or T. This molecular marker is accurate and efficient to detect, convenient and stable to amplify, and can be used for marker-assisted selection to improve the efficiency and accuracy of identifying the waterlogging stress resistance of wheat plants.

The application discloses a parent-offspring identification primer group and method of Prunus. The parent-offspring identification primer group of Prunus disclosed by the application is composed of 30 single-stranded DNAs shown in sequence 1 to sequence 30 in a sequence list. The primer group and method of the application can identify the genetic relationship of Prunus armeniaca, Prunus dulcis and their offspring, establish a parent-offspring identification system, and are simple to operate. The primer group and method of the application can quickly obtain genetic information of Prunus parents and offspring and determine the source of the offspring, can early determine and retain hybrid offspring between Prunus armeniaca and Prunus dulcis at the seedling stage, which is beneficial to quickly determine the true outcrossing seedling at the seedling stage, thereby removing hybrid offspring caused by natural pollination and eliminating the hybrid offspring at the seedling stage, so as to save land resources, reduce the identification cost in the later period, speed up the breeding process, and also provide a reference for parent-offspring identification of other fruit plants.

This invention discloses a rice blast resistance gene. Pilx SNP molecular markers, primer sets, and applications, wherein the SNP molecular markers are... Pilx The SNP molecular marker Pilx The polymorphic site is located on rice chromosome 12. Pilx The 18066332nd base of the gene is either T or C. This invention relates to a rice blast resistance gene. Pilx SNP molecular markers were used, and PARMS technology was applied to the reaction of rice materials. Pilx Genetic testing can rapidly and accurately detect rice blast resistance genes in different germplasm resources such as indica and japonica rice. Pilx .

This invention discloses SSR marker primers specific to the filamentous petals of gerberas and their applications. This invention utilizes SSR molecular markers to effectively identify different filamentous petal traits in gerberas, resulting in an SSR marker primer that can rapidly and accurately identify the petal traits of gerbera plants with different filamentous petals and the F1 generation of interspecific hybrids. The SSR marker primers provided by this invention can effectively distinguish between filamentous and non-filamentous petals in gerberas and exhibit significant polymorphism, with an accuracy greater than 65%. Furthermore, compared with traditional morphological trait observation methods, the identification method provided by this invention has better repeatability, saves time, and has high specificity. It can replace traditional morphological identification methods, effectively improving the molecular-level breeding process of gerberas and reducing the identification cycle of traditional morphological trait observation. It also allows for early screening to obtain gerbera offspring with superior filamentous varieties, which is of great significance for gerbera breeding.

This invention discloses a SNP locus associated with wheat salt tolerance and its application in predicting wheat salt tolerance, belonging to the field of molecular marker breeding technology. The SNP locus of this invention is located at position 1655 of the CDS region of the TaHKT1;1-B gene. When the base at this locus is G, the corresponding wheat is salt-tolerant; when the base at this locus is A, the corresponding wheat is not salt-tolerant. Based on this SNP locus, a method for predicting wheat salt tolerance has been developed, which can predict the maximum relative root length of wheat after salt stress, saving costs, improving selection efficiency, and thus accelerating the breeding process. This provides new potential opportunities for efficient screening of superior alleles and the breeding of salt-tolerant and drought-resistant wheat varieties.

The application relates to the fields of molecular biology and genetic breeding, and provides an application of a SNP molecular marker related to a body height trait of sheep. The SNP molecular marker is located at 4864599 bp of a sheep chromosome 16, and is a G>A base mutation (rs404408103). Experiments show that the SNP molecular marker developed in the application has a significant influence on the body height trait of sheep P <0.01>, and the allele A has a more excellent body height trait, so ERGIC1 The SNP molecular marker in the gene can be applied to sheep body height trait molecular marker breeding. The molecular marker provided by the application is not limited by the age and gender of the sheep, can be used for breeding excellent body height sheep varieties, and can be accurately screened even after birth, so that the breeding process of the excellent body height sheep varieties is greatly accelerated.

This invention discloses a method for delivering a VIGS vector targeting the flower buds inside the bulb of Lycoris radiata, a method for verifying gene function, and their applications, belonging to the field of biotechnology. The method includes: making 4-6 holes around the root plate of a dormant Lycoris radiata bulb, and 2-4 holes in the central area of ​​the bulb base, reaching the meristematic region of the flower bud; immersing the treated bulb in VIGS infection solution up to the wounds on the root plate; and performing vacuum treatment to ensure effective penetration of the infection solution into the internal flower bud tissue. This invention successfully delivered a silencing vector targeting the LrP5CS1 gene to the flower bud, specifically reducing its expression and obtaining a stable early-flowering phenotype in Lycoris radiata, thus verifying the gene function. Therefore, this invention establishes an efficient delivery platform that can be used for rapid screening and verification of other key genes regulating the flowering period and ornamental traits of Lycoris radiata, providing a novel technical means for the functional study of genes related to the flowering phenotype of Lycoris radiata.

This invention provides molecular markers for SNPs (Special Nominal Nuclei) of the logarithmic rib trait in Meihuaxing pigs and their applications. The specific base sequences of the SNP molecular markers are shown in SEQ ID NO. 23-33. These molecular markers are mainly used for screening and / or identification of superior breeds. The advantages of this invention are that, in actual breeding, retaining individuals carrying superior genotypes helps improve breeding efficiency and selection accuracy. Simultaneously, it can increase the gene frequency of the superior economic trait of multiple ribs in the population, allowing for targeted improvement of the rib logarithmic trait, thereby enhancing the carcass performance and economic benefits of the entire population.

The application discloses a molecular marker closely linked to relative root length of wheat and application thereof, and belongs to the technical field of molecular marker breeding. The molecular marker is located at the 530103940th position of chromosome 7 in a Chinese spring wheat reference genome sequence RefSeq v2.1, when the base at the position is G, the relative root length of the wheat is relatively long; when the base at the position is C, the relative root length of the wheat is relatively short. By applying the molecular marker developed in the application, the relative root length of the wheat can be predicted, cost is saved, selection efficiency is greatly improved, the breeding process can be accelerated, and new possibilities are provided for efficient screening of excellent alleles of a TaHKT2; 7-D gene and cultivation of salt-tolerant and drought-resistant wheat varieties.

The application relates to the technical field of genetic engineering, and discloses application of a CsC3'H gene in increasing contents of citrus naringin, hesperidin and / or jaceosidin, a CDS sequence of the CsC3'H gene is shown as SEQ ID NO. 1, and a protein sequence coded by the CsC3'H gene is shown as SEQ ID NO. 2. It is found for the first time that the CsC3'H gene is positively correlated with the contents of citrus naringin, hesperidin and / or jaceosidin, and the highest content of citrus naringin, hesperidin and / or jaceosidin in a citrus transiently transformed with a CsC3'H gene overexpression vector can be 69.38% higher than that in a citrus transformed with a blank vector; therefore, the CsC3'H gene can be used as a candidate gene for breeding of a new citrus variety with high contents of citrus naringin, hesperidin and / or jaceosidin, and has important significance for cultivating a functional new citrus variety by using a genetic engineering method, accelerating a breeding process, reducing a breeding workload and improving comprehensive utilization of citrus.

This invention relates to the field of plant breeding technology, and particularly to SNP loci related to the oil content of Camellia oleifera seed kernels in Hainan and their applications. The SNP loci include one or more of Chr27_83344296, Chr43_56056639, or Chr15_65268299; Chr27_83344296 is located at position 83344296 on chromosome 27, with a polymorphism of G / T; Chr43_56056639 is located at position 56056639 on chromosome 43, with a polymorphism of C / T; and Chr15_65268299 is located at position 65268299 on chromosome 15, with a polymorphism of G / A. By detecting the polymorphism of these SNP loci, the oil content of Camellia oleifera seed kernels in Hainan can be detected at the seedling stage, significantly reducing production costs and improving selection efficiency, thus possessing significant application value.