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Detection primers for molecular markers in close linkage with major QTL of wheat ear length and application of detection primers

A technology of molecular markers and detection primers, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc. Effects of Breeding Cycles

Inactive Publication Date: 2014-06-25
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] But currently there are few closely linked molecular markers for panicle length that can be used in practical molecular breeding

Method used

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  • Detection primers for molecular markers in close linkage with major QTL of wheat ear length and application of detection primers
  • Detection primers for molecular markers in close linkage with major QTL of wheat ear length and application of detection primers
  • Detection primers for molecular markers in close linkage with major QTL of wheat ear length and application of detection primers

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] A method for obtaining molecular markers closely linked to wheat ear length major QTLs, comprising the following steps:

[0053] (i) F 1 , F 1 Haploid was induced by crossing wheat and maize, and the DH population of 168 families was obtained through chromosome doubling.

[0054] (ii) Refer to the DNA extraction method provided by Triticarte Pty.Ltd (http: / / www.triticarte.com.au) to extract the DNA of each strain, and use simple repeat sequence markers (SSR markers) and simple repeats based on expressed sequence tags Sequence markers (EST-SSR markers), ISSR markers and high molecular weight glutenin (HMW-GS) subunits were used for genotype analysis of the strains to obtain the genotype data of the DH population.

[0055] Among them, the analysis of SSR markers, EST-SSR markers and ISSR markers is to amplify the DNA of the screened strains by PCR, and the amplified products are detected by 8% polyacrylamide gel electrophoresis. State primers, polymorphic primers betwe...

Embodiment 2

[0079] Shannong 19 and Shannong 20 were purchased from Shandong Shengfeng Seed Industry Technology Co., Ltd.; Shannong 8355 was purchased from Zibo Hefeng Seed Co., Ltd.; Huapei 3 and Yumai 57 were purchased from Henan Academy of Agricultural Sciences; Shannong 62008 and Shannong Nong 08-29 was purchased from the Quality Breeding Laboratory of the Grain Quality Testing Center of the Ministry of Agriculture, Shandong Agricultural University;

[0080] The application of a molecular marker tightly linked to the main effect QTL of wheat ear length in detecting the length of wheat ear, the steps are as follows:

[0081] (1) Extract the genomic DNA of the wheat to be tested, take 3-4 pieces of 10-day-old wheat leaves, put them in a 2mL centrifuge tube, put them in a container filled with liquid nitrogen, freeze them and grind them thoroughly, add 900μL to preheat at 65°C DNA extraction working solution, put in a water bath at 65°C for 1 hour, shake carefully several times during the...

Embodiment 3

[0092] Molecular markers closely linked to main QTL for wheat panicle length in backcross BC of crosses between Thipotinia elongatum and common wheat 3 f 2 Applications in groups:

[0093] A backcross BC was constructed by crossing E. elongatum as the donor parent and common wheat Shannong 20 as the recipient parent, and then using Shannong 20 as the recurrent parent for backcrossing. 3 f 2 population; the screening and verification of closely linked molecular markers for wheat panicle length major QTL was carried out. Specific steps are as follows:

[0094] (i) The obtained backcross BC of Echinopsis elongatum, Shannong 20 and 3 f 2 The population was planted in the field at the experimental station of Shandong Agricultural University, and the leaves of 300 offspring material plants were randomly selected to extract the DNA of each strain using the DNA extraction method provided by the above-mentioned Triticarte Pty.Ltd (http: / / www.triticarte.com.au) .

[0095] (ii) Pe...

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Abstract

The invention relates to detection primers for molecular markers in close linkage with a major QTL (Quantitative Trait Locus) of wheat ear length and an application of the detection primers. The detection primers for the molecular markers include an upstream primer, having a nucleotide sequence as shown in SEQ ID NO.1, of the molecular marker XWMC112 and a downstream primer, having a nucleotide sequence as shown in SEQ ID NO.2, of the molecular marker XWMC112, and an upstream primer, having a nucleotide sequence as shown in SEQ ID NO.3, of the molecular marker XCFD53 and a downstream primer, having a nucleotide sequence as shown in SEQ ID NO.4, of the molecular marker XCFD53. The molecular marker XWMC112 and the molecular marker XCFD53 are studied and found for the first time, and can be used for quickly determining whether a wheat variety or strain has the QTL of the ear length, and thus quickly screening out the wheat variety or strain having the QTL for increasing the ear length, and therefore, the breeding progress of a high-yield wheat variety can be accelerated.

Description

technical field [0001] The invention discloses a molecular marker detection primer closely linked with main effect QTL of wheat ear length and its application, and relates to the technical field of wheat molecular biotechnology and molecular marker assisted breeding application. Background technique [0002] Wheat is one of the most important food crops in the world, and its yield affects the development and stability of the national economy. Therefore, continuously increasing wheat yield has always been an important research direction of wheat. There are many factors affecting yield traits. In addition to the three factors of yield, ear length is also one of the important factors of wheat crop yield and one of the important traits of breeding objectives. Traditional breeding methods have the disadvantages of long cycle time, high cost, high cost, and small results in the selection of yield-related traits. With the development of molecular markers and biotechnology, molecula...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 田纪春邓志英崔勇李继发
Owner SHANDONG AGRICULTURAL UNIVERSITY
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