36 results about "Glutenin" patented technology

The invention discloses a bionic lignin-based saline-alkali soil improvement material and a preparation method thereof, and belongs to the technical field of high polymer materials and soil improvement. According to the saline-alkali soil improving material, lignin or a derivative thereof serves as a framework, glutenin charge distribution and a dynamic disulfide bond sodium ion response function are simulated, and the sequence and the proportion of chain segments of sulfonic acid groups and quaternary ammonium salt groups are accurately regulated and controlled through step-by-step activation and alternate copolymerization in an aqueous solution polymerization reaction system; positive and negative charges form more uniform sequence distribution, so that the amphipathy of the lignin compound is realized; then, sulfydryl is introduced through click chemistry, linear regulation and control of material viscosity and water solubility are achieved through the chain transfer characteristic of multi-sulfydryl functional groups, and the requirements of different saline-alkali soil application scenes are met. The limitation that a traditional lignin product is single in function is broken through, efficient utilization of lignin is achieved, and the intelligent saline-alkali soil improvement material integrating high stability and pH and Na < + > multi-environment response is developed.

The invention relates to the technical field of cell culture, in particular to an oriented porous bionic gelatin scaffold for controlling cell orientation arrangement, a preparation method and application thereof, and the oriented porous bionic gelatin scaffold can be used for cell culture meat production. The preparation method comprises the following steps: mixing and solidifying gelatin and glutamine transaminase, directionally freezing, freeze-drying, sterilizing, inoculating and culturing cells and the like. Parameters and culture medium components in each step are also explained. Compared with the prior art, a regular directional porous structure is formed through a hydrogel scaffold and directional freezing, directional arrangement and growth of fish muscle satellite cells are guided, the cell activity is high, and the proliferation and differentiation effects are good; the gelatin material is adopted, so that the cost is low, the biocompatibility is good, and the gelatin material is superior to collagen and wheat glutelin; the preparation process is simple, does not need complex equipment, is suitable for large-scale production, and promotes the development of cell culture meat.

The application discloses a desensitization wheat gluten peptide and a preparation method and application thereof, and belongs to the field of food deep processing. The desensitization wheat gluten peptide is obtained by modifying and screening wheat gluten by means of joint methods including enzymolysis, ultrasonic, ultrafiltration segmentation and microwave. The wheat gluten peptide is fine and has uniform granularity, color and flavor, and can be directly used as a functional food, and has wide application value in the field of food processing industry and snacks. The application constructs a compound nutrient powder by using the wheat gluten peptide and starch, and the processing and sensory characteristics, nutritional property and antioxidant property of the compound nutrient powder are improved, and the allergenicity is reduced, so that the compound nutrient powder meets the international gluten-free (<10ppm) food standard and can be applied to food industry low-gluten cereal food base. The combined processing of various food processing shows a good synergistic effect, improves the comprehensive performance of the food system, and provides technical guidance and a good production model for preparing gluten-free food with good color, fragrance and flavor and health.

The invention discloses an application of a TaSnRK1alpha gene in regulating and controlling the high-molecular-weight glutelin content of wheat. The high-molecular-weight glutelin content of the wheat is increased by increasing the TaSnRK1alpha gene in the wheat. In addition, the invention also discloses a TaSnRK1 alpha gene overexpression vector, the vector is obtained by introducing a pWMB110 recombinant vector containing a TaSnRK1 alpha-FLAG expression cassette driven by a Ubi promoter, the efficient expression of the TaSnRK1 alpha gene in wheat can be realized by utilizing the overexpression vector, and the high molecular weight glutelin content in mature grains of the TaSnRK1 alpha gene is obviously improved compared with that of a wild type. New germplasm resources and efficient technical path support are provided for breeding of the high-gluten wheat, the breeding period is shortened, and the method has important agricultural application value.

The invention discloses a method for preparing functional food with high gamma-aminobutyric acid content by fermenting glutenin bean sprout juice zymolyte and coix seeds through monascus. The method comprises the following steps: dissolving vital gluten in 65% ethanol according to a ratio of 1: 30, stirring and extracting at 35 DEG C for 2 hours, standing and separating precipitate and supernate, continuously extracting the precipitate with 65% ethanol, and centrifuging after extraction to obtain the precipitate, namely glutenin. Soaking the mung beans at 25-35 DEG C to germinate the mung beans, and juicing the mung beans when the mung beans grow to 5-10 cm; mixing the mung bean sprout juice and glutenin, performing enzymolysis reaction, drying and crushing. Mixing coix seeds and glutenin, and sterilizing to obtain a solid culture medium to be fermented; spores on the monascus purpureus slant culture medium are dissolved out with sterile water and inoculated to a solid culture medium, and the solid culture medium is placed in a constant-temperature incubator to be fermented after being evenly mixed. And drying the fermented culture medium at 60 DEG C until the weight is constant, and crushing. The content of gamma-aminobutyric acid in the food with high gamma-aminobutyric acid content is higher than 2.5 mg / g.

The invention discloses a method for extracting and identifying a high molecular weight glutenin subunit (HMW-GS) in wheat grains in an early stage, which comprises the following steps: taking the wheat grains 15 days after flowering and before a dough stage, and extracting by using 80-160 [mu] l of an extraction buffer solution (40% of isopropanol, 200mM of Tris-HCl, pH 8.0, 0.75% of DTT and 0.5% of 4-VP). The extraction and identification method is suitable for early identification of high molecular weight glutenin subunit types in wheat grains, and is also suitable for identification of glutenin subunit types in harvested seeds. The seeds 15 days after flowering and before the grain dough stage are high in moisture content, low in grain hardness and easy to grind, early sampling accelerates the extraction and detection efficiency of the wheat grain high-molecular-weight glutenin subunit, and accelerates the wheat quality breeding process.

The invention provides a preparation method of a mixed biomass carbon coated silicon-based negative electrode material. Biomass carbon is mainly derived from prolamin, glutenin and high-activity yeast. Under the catalysis of high-activity yeast, prolamin and glutenin meet water to form a net-shaped structure, and the flexibility of the carbonized porous negative electrode material can be adjusted through different addition proportions, so that the flexible porous nitrogen-doped carbon-coated silicon-based negative electrode material can be obtained, the problem of volume expansion in the electrochemical process of a silicon negative electrode is relieved, and meanwhile, the conductivity is improved; the excellent electrochemical performance is shown. The porous silicon-based negative electrode material with controllable flexibility is obtained through drying and high-temperature annealing, the method is simple, effective and low in cost, and the prepared composite silicon-based negative electrode material is excellent in performance and suitable for large-scale production.

The invention relates to the technical field of food production, in particular to an automatic wheat gluten production system and a production method thereof.The production method comprises the following steps that wheat flour and water are put into a box; the main shaft rotates to drive the spiral blade to rotate, wheat flour is stirred into dough, and the dough gathers into a working cavity in the box body; acquiring the working state of the part, close to the working cavity, of the spiral blade; judging whether the current working state of the helical blade needs to adjust the volume of the working cavity and the spindle speed; when the volume of the working cavity and the rotating speed of the spindle need to be adjusted, the volume of the working cavity in the box and the rotating speed of the spindle are adjusted based on parts, close to the working cavity, of the spiral blades. According to the application, the volume of the area where the dough is located and the output power of the motor are adjusted in real time by implementing a mode of obtaining the working state of the spiral blade, and the stirring intensity is controlled based on the state of the dough, so that the denaturation probability of glutenin and gliadin during production is effectively reduced, and the product quality is improved.

The invention provides low-GI functional noodles based on a biological enzymolysis-microwave puffing technology and a preparation method of the low-GI functional noodles, and belongs to the technical field of food processing. The low-GI fermented tea functional noodles comprise the following raw materials in percentage by mass: 60%-80% of high gluten wheat flour, 10%-25% of resistant starch, 5%-15% of functional tea powder, 3%-8% of active wheat glutelin and 0.5%-1% of table salt. The functional tea powder is porous particles which are prepared from fermented tea through enzymolysis for regulating and controlling the viscoelastic state and microwave vacuum puffing and have a honeycomb-shaped open-type micropore structure. According to the invention, a'structured remodeling 'strategy is creatively adopted, so that the defects that a gluten network is damaged by traditional tea powder as a compact hard filler and the thermal stability of a liquid extract is poor are overcome. The functional tea powder is added, so that the noodles disclosed by the invention have excellent boiling fastness on one hand, and have excellent and stable low-GI characteristics on the other hand.

The preparation method comprises the following steps: mixing vital gluten and 65% ethanol according to a mass ratio of 1: 30, and stirring and extracting in a water bath kettle at 35 DEG C for 3 hours to dissolve alcohol-soluble protein in the vital gluten in the ethanol; after standing, centrifuging for 5 minutes by using a centrifugal machine under the condition of 3,500 r / min, taking the precipitate, repeating the steps for dissolving, centrifuging the solution for 5 minutes, and taking the precipitate, namely the glutenin aggregate. Dissolving the glutenin aggregate at 30 DEG C for 120 minutes by using 0.1% sodium hydroxide alkali liquor, standing at 50 DEG C for 12 hours, and regulating the pH value to be neutral to generate precipitate; and centrifuging for 5 minutes to obtain a precipitate which is alkali-soluble glutenin. Amylose and alkali-soluble glutenin are mixed according to the mass ratio of 1: 8-1: 10, drinking water accounting for 3%-5% of the starch amount is added, mixing is conducted for 30-50 min at the temperature of 30-35 DEG C, and the disulfide bond content of the alkali-soluble glutenin is reduced by 80% or above.

The invention discloses a TaMYB44 gene overexpression vector and application of the TaMYB44 gene overexpression vector, the vector takes pWMB110 as a skeleton vector, and comprises an expression cassette for regulating TaMYB44-FLAG expression by a Ubi promoter. The wheat vector with the function of overexpressing the TaMYB44 gene is constructed, and the TaMYB44 gene in the wheat is overexpressed through genetic transformation, so that the content of high-molecular-weight glutelin in the wheat can be remarkably reduced, a material is provided for wheat quality breeding, and the wheat vector can be used for creating a weak gluten wheat germplasm material.

The invention discloses a molecular marker and a primer group for detecting high-molecular-weight glutenin subunits of wheat and application of the molecular marker and the primer group, and belongs to the technical field of molecular marker-assisted breeding. The nucleotide sequence of the molecular marker is as shown in SEQ ID NO.3, and T / A mutation exists at the 21st base of the molecular marker; the wheat high-molecular-weight glutenin subunit of which the mutation site is T is Ax1, and the wheat high-molecular-weight glutenin subunit of which the mutation site is A is Ax2 *. The specific KASP molecular marker is developed on the basis of variation sites of Ax1 and Ax2 * subunit gene coding regions in a wheat genome, the Ax1 and Ax2 * subunit types of the wheat high-molecular-weight glutenin can be detected by utilizing the molecular marker, the identification result is accurate and reliable, the repeatability is good, the identification process of the high-molecular-weight glutenin subunit in wheat offspring is greatly simplified, and the application prospect is wide. And the identification efficiency and accuracy are improved.

The invention belongs to the field of battery materials, and discloses a nickel positive electrode material and a preparation method and application thereof.The preparation method comprises the steps that a polycrystalline precursor of NixM1-x (OH) 2, a lithium source, glutenin and gliadin are ground and mixed, and mixed powder is obtained; mixing the solvent and the mixed powder to obtain slurry, and drying to obtain a mixture; and pre-sintering the mixture in an oxygen atmosphere, and then heating and roasting for a certain time to obtain solid particles, namely the nickel-based positive electrode material. By adding glutenin and gliadin and combining with a roasting process, primary crystal grains of a polycrystalline precursor of NixM1-x (OH) 2 continuously grow in the roasting process, so that the prepared nickel-based positive electrode material is of a submicron single-crystal structure, the specific surface area of the positive electrode material is reduced, the possibility of side reaction is reduced, and the service life of the positive electrode material is prolonged. The structural stability of the positive electrode material can be further improved, the cycle performance of the battery is finally improved, and the volume energy density of the battery is increased.

Provided herein is a protein composition that is free of wheat gluten, comprising one or more isolated, enriched, or purified gluten-like proteins from non-wheat sources, wherein the gluten-free protein composition forms a gluten-like network in food products containing the gluten-free protein composition. The gluten-free protein composition may serve as a gluten replacement by, for example, promoting a volume rise of at least 100% during proofing and baking, a density of 0.75 g / cm3 or lower, and / or a moisture of at least 25% after baking in a gluten-free bread. Also provided herein are food products containing the gluten-free protein composition, and methods of producing the gluten-free protein composition and the food products.

The invention relates to a dry-extruded composition comprising plant proteins excluding hydrolyzed wheat gluten, preferably legume proteins, preferably pea proteins, as well as hydrolyzed wheat gluten protein, a method for producing same and the use thereof.

The invention relates to the technical field of crop breeding, and particularly discloses a wheat translocation chromosome and a molecular marker and application thereof, and the translocation chromosome comprises a 1AS. 1AL-1DLDx5 + Dy10 translocation chromosome formed by replacing a 475.8-598.6 Mb segment of a long arm end of a 1A chromosome with a 376.6-498.6 Mb segment of a wheat 1D chromosome long arm end. According to the invention, a 1AS.1AL-1DLDx5 + Dy10 translocation chromosome containing a Dx5 + Dy10 high-quality high-molecular-weight glutenin subunit gene is created, and a matched molecular marker is developed, so that the problems that in existing wheat breeding, excellent genes are lacked, specific translocation line identification depends on a time-consuming and labor-consuming cytological method, and high-throughput application is difficult are solved; according to the method, a 1AS.1 1AL-1DLDx5 + Dy10 translocation chromosome carrier and a non-carrier are rapidly and accurately distinguished, traditional cytological identification is replaced, molecular marker-assisted breeding is supported, and the breeding process of a new variety of high-quality wheat is accelerated.

The invention discloses a CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 (CRISPR associated protein 9) gene editing vector targeting a wheat TaSnRK1 alpha gene and application of the CRISPR / Cas9 gene editing vector. PBUE414 is used as a skeleton vector of the vector, and the vector comprises a Cas9 expression cassette and an sgRNA (single guide ribonucleic acid) expression cassette; the Cas9 expression cassette is an expression cassette for regulating and controlling Cas9 expression by a Ubi promoter, and the sgRNA expression cassette sequentially comprises the following elements from upstream to downstream: a TaU3 promoter, sgRNA1 of TaSnRK1 alpha, a terminator OsU3t, a TaU3 promoter, sgRNA2 of TaSnRK1 alpha and a terminator TaU3t. The vector is suitable for reducing the high-molecular-weight glutelin content of wheat mature grains through a gene knockout technology, and a new technical means and germplasm resources are provided for wheat quality improvement.

The invention belongs to the technical field of food manufacturing, and particularly relates to alum-free high-gluten vermicelli and a preparation technology thereof.The preparation technology comprises the steps that potato starch is taken and mixed with water, then the mixture is stirred and gelatinized in a boiling water bath, and thick starchy sauce is prepared; adding oxidized potato starch, modified glutenin and water into the starchy sauce paste, and kneading into soft dough; extruding the soft dough into vermicelli; the vermicelli falls into boiling water, the vermicelli is fished out after 10-15 s, then the vermicelli is immediately transferred into a cold water pool, the vermicelli is bundled after being cooled, the vermicelli is placed in an environment with the temperature of 0-4 DEG C and the humidity of 60-80% for 12-16 h, then the vermicelli is frozen at the temperature of-18 DEG C, unfrozen and dried, and the alum-free high-gluten vermicelli is obtained. According to the invention, the oxidized potato starch and the modified glutenin are combined, the gluten power of the vermicelli is improved and the breakage rate of the vermicelli is reduced through the modified glutenin, and meanwhile, the gelatinization effect of the starch is improved and the gelatinization inhibition effect of the modified glutenin on starch particles is weakened through slight oxidation of the potato starch.

The present application belongs to the technical field of skin care products, and particularly relates to a PDRN anti-aging composition, a preparation method thereof and application. The present application uses hydrolyzed sponges to open the absorption channels of the stratum corneum, and uses the specific proteins, polypeptides in hydrolyzed royal jelly protein and wheat glutelin and PDRN to form a stable molecular structure through van der Waals force, so as to enhance the stability of PDRN and promote the activity of PDRN. The high-energy phosphate bond of sodium mannose phosphate can enhance the activity of mitochondria, provide more energy for the penetration and absorption of PDRN and the exertion of efficacy, and tetrahydro methyl pyrimidine carboxylic acid is used to relieve the irritation of hydrolyzed sponges. Therefore, the PDRN anti-aging composition provided by the present application has a synergistic effect between the components. The data of the examples show that the PDRN anti-aging composition provided by the present application has good penetration and anti-aging effect.

The invention provides a method for cultivating wheat suitable for processing cakes, which comprises the following steps of: hybridizing wheat materials deleted by different high-molecular-weight glutenin and wheat materials deleted by different alcohol-soluble proteins, carrying out molecular marker-assisted selection on offspring to obtain a series of new deletion bodies deleted by the high-molecular-weight glutenin and the alcohol-soluble proteins, and carrying out high-molecular-weight glutenin and alcohol-soluble protein co-deletion. Through cake processing quality determination, the gluten protein deletion body with excellent cake processing quality is screened out.

The invention discloses a method for predicting freezing resistance of wheat frozen dough based on a multiplex PCR molecular marker technology, and belongs to the technical field of molecular biology. According to the invention, a specific multiplex PCR system is constructed, and a high-molecular-weight glutenin subunit combination related to dough freezing resistance is synchronously identified. The method provided by the invention comprises the following steps: extracting wheat genome DNA by using an improved CTAB method; and carrying out synchronous amplification and identification on the subunits of the sites Glu-A1 and Glu-D1. According to the invention, efficient and rapid integrated detection of a plurality of high-quality subunits is realized, and reliable technical support is provided for directional breeding of wheat varieties with excellent frozen dough quality.

The invention provides blood-glucose-reducing whole-grain rhizoma polygonati noodles and a preparation method thereof. The blood-glucose-reducing whole-grain rhizoma polygonati noodles comprise the following raw materials: 50-70 parts of whole-grain flour, 20-40 parts of wheat flour, 1-10 parts of blood-glucose-reducing paste and 1-10 parts of a rhizoma polygonati polysaccharide extract, the blood glucose reducing paste is prepared from the following raw materials: 28 to 32 parts of rhizoma polygonati, 13 to 17 parts of radix astragali seu hedysari, 8 to 12 parts of rhizoma dioscoreae, 8 to 10 parts of herba dendrobii, 10 to 14 parts of rhizoma polygonati odorati, 4 to 6 parts of radix puerariae, 8 to 10 parts of semen euryales and 3 to 5 parts of endothelium corneum gigeriae galli. According to the method, liquid nitrogen crushing and high-pressure homogenization are adopted, so that whole wheat crude fibers can be fully crushed, and the roughness caused by different sizes of the fibers is reduced; through low-temperature enzymolysis, sulfur groups of glutenin and gliadin are promoted to be oxidized into disulfide bonds to generate crosslinking, so that the toughness and the tenderness of dough are improved, the problem that soup is easy to muddy in the noodle cooking process is solved, and meanwhile, the natural breaking rate, the cooked breaking rate and the cooking loss rate of the noodles are reduced.

This invention discloses a plant glutenin sorting-related protein, OsGPA17, its encoding gene, and its applications. This invention utilizes the rice glutenin 57H mutant. gpa17 Phenotypic analysis and preliminary localization of the target gene led to the cloning of the protein sorting-related protein OsGPA17. This protein consists of the amino acid sequence shown in SEQ ID NO.1, and the nucleotide sequence of the gene is shown in SEQ ID NO.2 or SEQ ID NO.3. The gluten sorting-related protein of this invention affects the gluten sorting process in rice endosperm. Introducing the encoding gene of this protein into plants with reduced mature protein content yields transgenic plants with normal mature protein content. Therefore, the protein and its encoding gene of this invention can be applied to plant genetic improvement.

The purpose of the present invention is to provide a food product having improved stickiness and meltability in the mouth as compared with food products produced from GA-SX wheat flour.　The problem is solved by altering the allele at a high-molecular-weight glutenin gene Glu-D1 of GA-SX wheat (a wheat in which the enzymatic activity of GBSSI-A1 is not deficient, the enzymatic activities of GBSSI-B1 and GBSSI-D1 are deficient, and the enzymatic activities of any two of SSIIa-A1, SSIIa-B1 and SSIIa-D1 are deficient) to a d-type allele.

A plant-based cooked meat imitation product is prepared by hydrating non-glutelin vegetable proteins, then mixing a plant-based oil and hydrocolloid, active wheat glutelin, and edible acid to form a plant-based dough, forming the plant-based dough into dough segments and cooking to an internal temperature of at least about 180 DEG F, to form a plant-based cooked meat-like product. By selecting specific ingredients in an effective ratio and mixing these ingredients in a specific order, a plant-based cooked meat-like product having an excellent appearance and texture can be prepared.

The invention discloses a Glu-1Ey gene for regulating and controlling wheat protein content, a protein and application of the Glu-1Ey gene. The nucleotide sequence of the Glu-1Ey gene is shown as SEQ ID No. 1. According to the invention, an overexpression vector for transforming the agropyron elongatum Glu-1Ey gene is successfully constructed by using a gene overexpression technology, and a strain for overexpressing the Glu-1Ey gene is obtained by using an agrobacterium-mediated wheat immature embryo genetic transformation method. The protein content analysis of transgenic wheat proves that the high-molecular-weight glutelin y-type subunit gene Glu-1Ey of elytrigia elongata can improve the protein content and glutelin content of wheat. The technical scheme provided by the invention has important significance for obtaining high-quality wheat new germplasm by utilizing the high-molecular-weight glutelin y-type subunit gene Glu-1Ey of elytrigia elongata.

The application discloses a kind of bionic lignin-based saline-alkali land improvement materials and preparation method, belong to polymer material and soil improvement technical field.The saline-alkali land improvement material of the present application uses lignin or its derivative as skeleton, simulates the charge distribution of wheat glutelin and the sodium ion response function of dynamic disulfide bond, and through step-by-step activation and alternating copolymerization in aqueous solution polymerization reaction system, the sequence and proportion of sulfonic acid group and quaternary ammonium salt group segment are accurately controlled, so that positive and negative charges form more uniform sequence distribution, realize the amphiphilicity of lignin compound;Then introduce thiol group using click chemistry and realize the linear regulation of material viscosity and water-soluble using the chain transfer characteristics of multi-thiol functional group, to meet the needs of different saline-alkali land application scenarios.The present application breaks through the limitation of traditional lignin product single function, realizes the efficient use of lignin, and develops high stability, pH and Na + The application discloses a kind of bionic lignin-based saline-alkali land improvement materials and preparation method, belong to polymer material and soil improvement technical field.The saline-alkali land improvement material of the present application uses lignin or its derivative as skeleton, simulates the charge distribution of wheat glutelin and the sodium ion response function of dynamic disulfide bond, and through step-by-step activation and alternating copolymerization in aqueous solution polymerization reaction system, the sequence and proportion of sulfonic acid group and quaternary ammonium salt group segment are accurately controlled, so that positive and negative charges form more uniform sequence distribution, realize the amphiphilicity of lignin compound;Then introduce thiol group using click chemistry and realize the linear regulation of material viscosity and water-soluble using the chain transfer characteristics of multi-thiol functional group, to meet the needs of different saline-alkali land application scenarios.The present application breaks through the limitation of traditional lignin product single function, realizes the efficient use of lignin, and develops high stability, pH and Na + Multiple environment response integrated intelligent saline-alkali soil improvement material.

A de-epitoped low molecular weight glutenin subunits (LMW-GS) is provided. Methods of generating same and uses thereof are also provided. A de-epitoped LMW-GS may be beneficial for a subject suffering from gluten sensitivities, such as a celiac disease patient. A de-epitoped LMW-GS may be comprised in food or beverage products such as bread, cake, cookie, noodle, pasta, or pizza.

The application belongs to the technical field of food manufacturing, and particularly relates to a kind of high-strength vermicelli without alum and a preparation process thereof. Potato starch is mixed with water, and then stirred and gelatinized in a boiling water bath to form a thick paste. Oxidized potato starch, modified gluten and water are added to the paste to knead a soft dough. The soft dough is extruded into vermicelli. The vermicelli is taken out after 10-15 seconds in boiling water, and then immediately transferred into a cold water pool for cooling. After cooling, the vermicelli is bundled, and then placed in an environment with a temperature of 0-4 DEG C and a humidity of 60-80% for 12-16 hours. The vermicelli is then frozen at a temperature of -18 DEG C. After thawing, the vermicelli is dried to obtain high-strength vermicelli without alum. In the application, the combination of oxidized potato starch and modified gluten improves the strength of the vermicelli and reduces the breakage rate of the vermicelli. At the same time, the mild oxidation of the potato starch improves the gelatinization effect of the starch and weakens the gelatinization inhibition of the modified gluten on the starch particles.

This invention discloses a method for creating high-molecular-weight glutenin subunit mutants using single-base editing and its applications. The method includes the following steps: constructing a single-base editing vector by designing sgRNA targeting the tyrosine codon of the HMW-GSs gene; achieving wheat genetic transformation using Agrobacterium-mediated transformation; realizing targeted single-base substitution at target sites (such as position 70 of the 1Bx20 gene and position 73 of the 1Dx5 gene, TAC→TGC), precisely mutating tyrosine to cysteine; and determining bread-processing-related quality parameters of the mutants. This invention uses an adenine base editor to perform targeted editing of the tyrosine codon in wheat HMW-GSs, precisely mutating it to cysteine. This invention is the first to achieve precise editing of HMW-GSs tyrosine to cysteine ​​in wheat, providing a directly applicable theoretical basis and technical path for creating high-quality new wheat germplasm and optimizing bread production processes.