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Method for cryopreserving NK cells

A technology of NK cells and cryopreservation, applied in the biological field, can solve the problem that the protective effect of NK cells cryopreservation has not been reported yet.

Active Publication Date: 2019-06-14
内蒙古蒙科干细胞基因医学研究有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

5-Glutinen-3-ol (5-Glutinen-3-ol, CAS#:545-24-4) is the first substance isolated from wheat grains, and its protective effect on NK cells has not been reported yet

Method used

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  • Method for cryopreserving NK cells
  • Method for cryopreserving NK cells
  • Method for cryopreserving NK cells

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Embodiment Construction

[0021] The following describes the substantive content of the present invention in conjunction with the accompanying drawings and embodiments, but does not limit the protection scope of the present invention.

[0022] 1. Experimental materials

[0023] Lymphocyte separation medium was purchased from Beijing Suolaibao Technology Co., Ltd.; rhIL-2 was purchased from Xiamen Tebao Bioengineering Co., Ltd.; human AB plasma was purchased from Shanghai Huiying Biotechnology Co., Ltd.; SCGM serum-free medium was purchased from Shanghai Micro Branch Biotechnology Co., Ltd.; DMSO was purchased from Aladdin; FITC-Anti-CD56, PE-Anti-CD3, APC-Anti-CD107a were purchased from BD Company of the United States; Glutinen-3-ol, CAS#:545-24-4) purity ≥ 98%; CCK-8 reagent was purchased from Biyuntian; 0.4% trypan blue solution was purchased from Beijing Suolaibao Technology Co., Ltd.; MKN-45 human gastric cancer Cells were purchased from Shanghai Kanglang Biotechnology Co., Ltd.

[0024] 2. Exper...

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Abstract

The invention discloses a method for cryopreserving NK cells. The invention finds that 5-glutenin-3-alcohol has a cryopreserving effect to NK cells, is capable of increasing -80 DEG C cryopreserving recovery rate of NK cells and maintaining killing activity and lethality to tumor cells of NK cells after -80 DEG C cryopreserving recovery and does not change phenotype of NK cells. Thus, 5-glutenin-3-alcohol can be prepared into a cryopreserving protective agent for NK cells.

Description

technical field [0001] The invention belongs to the biological field and relates to cryopreservation of immune cells, in particular to a method for cryopreservation of NK cells. Background technique [0002] As an emerging tumor treatment method, tumor cell immunotherapy technology has the characteristics of broad spectrum of tumor killing recognition, high anticancer lethality, and less toxic and side effects. Natural killer cells (NK) can not be limited by MHC molecules through the interaction between their own surface activating receptors and tumor cell surface ligands, and then recognize tumor cells with down-regulated or missing expression of MHC-I molecules and exert their killing function. Since NK cells occupy a small proportion in peripheral blood, accounting for 5% to 15% of lymphocytes, and the function of NK cells in tumor patients is missing to varying degrees, it is difficult to produce good anti-tumor effects, and it is necessary to increase NK cells through i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 石学兵杨浩
Owner 内蒙古蒙科干细胞基因医学研究有限公司
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