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Application of atractylenolide IV to preparation of CIK cell cryopreservation solution

A technique of atractylidene and cryopreservation solution, which is used in CIK cell cryopreservation solution, and atractylodes lactone IV is used in the field of preparing CIK cell cryopreservation solution, which can solve the problem of not finding atractylodes lactone and so on.

Active Publication Date: 2018-01-16
SHANDONG XINRUI BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The applicant found that each Atractylodes lactone has different activities. After searching, no report was found that Atractylodes lactone IV was used to prepare CIK cell cryopreservation solution

Method used

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  • Application of atractylenolide IV to preparation of CIK cell cryopreservation solution
  • Application of atractylenolide IV to preparation of CIK cell cryopreservation solution
  • Application of atractylenolide IV to preparation of CIK cell cryopreservation solution

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Embodiment Construction

[0019] The substantive content of the present invention will be specifically introduced below in conjunction with the embodiments. Due to space reasons, the description of the experimental process cannot be done in great detail. All the parts not described in detail in the experiment are routine operations well known to those skilled in the art.

[0020] 1. Experimental materials

[0021] Atractylodes IV, Atractylodes I and Atractylodes III are self-made, and the purity is greater than 98%.

[0022] 2. Experimental method

[0023] 1. Isolation of peripheral blood mononuclear cells (PBMCs)

[0024] Peripheral anticoagulated whole blood was collected from healthy volunteers, added to Ficoll-Plaque lymphocyte separation medium and subjected to density gradient centrifugation (2500r / min, 25min), the buffy coat was extracted, and washed 3 times with PBS.

[0025] 2. Cultivation and expansion of CIK cells

[0026] Suspend PBMC in RPMI 1640 complete culture medium (containing 10% ...

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Abstract

The invention discloses application of atractylenolide IV to preparation of a CIK cell cryopreservation solution. The CIK cell cryopreservation solution comprises a serum-free medium, serum, dimethylsulfoxide and atractylenolide IV, wherein the serum-free medium is RPMI 1640; the serum is fetal calf serum or autoserum of CIK cell donors; preferably, the autoserum of CIK cell donors, the serum-free medium, the serum and the dimethyl sulfoxide are mixed according to a volume ratio of 5 to 4 to 1. The application of the atractylenolide IV to the preparation of the CIK cell cryopreservation solution shows that the atractylenolide IV is capable of increasing the cryopreservation recovery rate of the CIK cells without influencing the killing activity of the CIK cells on the tumor cells, and canbe prepared into the CIK cell cryopreservation solution for cryopreservation of the CIK cells so as to ensure that enough CIK cells are infused back to patients after the patients receive radiotherapy and chemotherapy.

Description

technical field [0001] The invention belongs to the field of cell cryopreservation and relates to a CIK cell cryopreservation solution, in particular to the use of atractyloid IV for preparing the CIK cell cryopreservation solution. Background technique [0002] Cryopreservation is the most commonly used means of cell storage, and most tumor cells still maintain their original characteristics after cryopreservation and recovery. For heterogeneous cells artificially induced and cultured such as CIK, the freezing time and system will have a certain impact on their immunophenotype and killing activity (reference: Effects of different freezing times on the immune phenotype and killing activity of CIK cells , Modern Oncology Medicine, 2016; Experimental study on resuscitation and culture of CIK cells from peripheral blood stem cells cryopreserved in different ways, Modern Diagnosis and Therapy, 2014; Effect of cryopreservation on cytokine-induced killer cell immunophenotype and i...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 王莉胡芳关凯
Owner SHANDONG XINRUI BIOTECH CO LTD
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