The invention discloses a killing method for identifying gamma delta T cells simply and rapidly. The method comprises the following steps: firstly, healthy volunteer peripheral blood is collected, separation is carried out by utilization of a human lymphocyte separating medium, mononuclear cells are obtained, phosphate antigens HMBPP with 10-30ng / ml and rhIL-2 factors with 500-1000 IU / ml are added, PBMC induction into gamma delta T cells is carried out and amplification culture is carried out; secondly, after culture is carried out for 8-10 days, gamma delta T cells are subjected to detection of destruction experiments, the living cell dye calcein-AM is employed to dye tumor cells, the cell density during dyeing is controlled at 10<5>-10<7> / ml; thirdly, the tumor cells after dyeing and the gamma delta T cells are cultured together for a period of time, the cells are collected, propidium iodide is added for dyeing before detection, finally, a flow cytometer is employed to detect cell death amount, and the cell killing rate is obtained. Through the method that the cell killing efficiency can be detected rapidly through a flow cytometer, limitation conditions of traditional 51Cr radioactive substance pollution and low sensitivity of MTT and LDH are overcome.