31 results about "Intestinal type" patented technology

Objective methods for detecting and diagnosing intestinal-type gastric cancers are described herein. Also described is method for predicting the presence or absence of lymph node metastasis (i.e., identifying the metastatic phenotype). In one embodiment, the diagnostic method involves the scoring of gene expression profiles that discriminate between lymph node positive tumors and lymph node negative tumors. The predictive score calculated acts as diagnostic indicator that can objectively indicate whether a sample tissue has the metastatic phenotype. The present invention further provides methods of diagnosing intestinal-type gastric cancer in a subject, methods of screening for therapeutic agents useful in the treatment of intestinal-type gastric cancer, methods of treating intestinal-type gastric cancer and method of vaccinating a subject against intestinal-type gastric cancer.

The invention discloses a detection method for a human intestinal flora. The method comprises the following steps: firstly, collecting a large number of human excrement samples, processing each excrement sample to obtain intestinal flora information, and then constructing an intestinal pattern and a flora diversity distribution trend chart according to the intestinal flora information of each sample; and determining the intestinal pattern of a to-be-detected sample according to intestinal flora information of the to-be-detected sample and the intestinal pattern constructed in a system, and comparing the intestinal flora information of the to-be-detected sample with the flora diversity distribution trend chart in the system to obtain a detection result of the to-be-detected sample, therebyrealizing detection of the intestinal flora. According to the invention, the detection of the human intestinal flora can be realized, so help and reference are provided for further applying the detection of the human intestinal flora to obesity detection and research. The invention further provides a detection device for the human intestinal flora and a storage medium.

The invention discloses a construction method of a Prevo intestinal type in-vitro simulation model. The fresh feces of the Prevo-intestinal healthy volunteers are diluted with PBS into a fecal suspension by using an automatic intestinal flora in-vitro simulation model apparatus, and the fecal suspension is inoculated into a modified VI medium, and fermentation culture is carried out under conditions of 37 DEG C, pH of 5.2-5.8, magnetic stirring speed of 150 rpm, and supplemented addition with a medium at a rate of 330 ml/12 h to obtain the Prevo intestinal type in-vitro simulation model. The method finds an important nutritional condition for simulating the Prevo intestinal type in-vitro, explores the physical and chemical conditions in the continuous fermentation process, and can increasethe proportion of Prevo bacteria in a broth to 40% to 80%, and the correlation coefficient between the microbial population in the broth and an original stool sample is about 80%, so that the Prevo intestinal type can be stably simulated in vitro.

Methods and compositions for detecting tumor origin are disclosed. In certain embodiments, the origin is determined by detecting expression levels of pan-epithelial membrane mucin (MUC1), intestinal-type secretory mucin (MUC2), cytokeratin 17 (CK17), or a combination thereof. Also disclosed are methods for aiding in the determination of an appropriate course of treatment for a subject with a tumor, or for predicting a therapeutic outcome of a subject, based on the expression levels of MUC1, MUC2, CK17, or a combination thereof. Kits for use in each of these methods are also provided.

The invention discloses a hair ball removing nutrient solution and a preparation method thereof. The hair ball removing nutrient solution is prepared from the following components: plantain seed shell, fried semen raphani, fructus cannabis, cortex magnoliae officinalis, plantain seed shell cellulose and black fungus. Pet cats are used to lick their own hair to clean themselves, but at the same time, the pet cats eat a lot of dead hair which is accumulated in the stomach and intestine, and if the pet cats do not spit out in time, intestinal tracts may be blocked. Therefore, a formula is provided, the plantain seed shell is used as a monarch material to relax bowel, absorb water in small intestines to form pasty blocks, improve the lubricating conditions of excrement and intestines and promote the defecation speed; fried semen raphani, fructus cannabis and cortex magnoliae officinalis are used as ministerial drugs to promote digestion and relieve stasis; the semen plantaginis cellulose and the black fungus are also known as intestinal tract cleaners, and the black fungus contains insoluble dietary fibers to help the monarch drug to discharge hair smoothly, so that the situation that the stomach is hurt and discomfort are caused due to the fact that partial hair discharge products in the market vomit from the mouth to discharge hair after the catnip is orally taken is avoided, and the effects of relaxing bowel, promoting digestion, relieving indigestion and discharging hair are achieved by combining all the raw materials.

The invention provides application of propylene glycol to medicine for preventing intestinal type radiation disease and radiation enteritis. According to the application disclosed by the invention, the propylene glycol is firstly applied to the field of radiation protection research, and the research proves that compared with former prevention application, the propylene glycol has an obvious experimental therapeutic effect on acute radiation injury of intestinal tracts and has curative effects on intraperitoneal injection and oral administration; moreover, a radiation protection mechanism of the propylene glycol is possibly related with removal of hydroxyl radicals through the propylene glycol and alleviation of intestinal cell DNA injuries. At present, the phenomenon that gastrointestinaltissue injuries or the radiation enteritis occurs when the propylene glycol is applied to prevention of ionizing radiation or tumor radiotherapy is not reported yet.

The invention belongs to the field of traditional Chinese medicine processing, and discloses a preparation method of an excrement inoculum substitute in a preparation process of rulvis glycyrrhizae praeparata. The preparation method comprises the following steps of (1) preparing an excrement inoculation liquid, namely, adding 0.1 M of sterilized and anaerobic treated PBS (Phosphate Buffer Solution) with the pH value of 7.0 into excrement, carrying out uniform vortex mixing, passing through a filtering membrane, and taking filtrate to obtain the excrement inoculation liquid; (2) determining the enterotypes of the intestinal microecology of the excrement inoculation liquid; (3) inoculation, namely, adding the excrement inoculation liquid into a fermentation culture medium in a fermentation tank, performing anaerobic fermentation, and performing in-vitro culture of enteric microorganisms to obtain fermentation liquid containing the enteric microorganisms; (4) continuously fermenting for 10-12 days, and collecting fermentation liquid when florae in the fermentation tank are stable; and (5) wrapping licorice powder with a semipermeable membrane (with the aperture of 2-5 microns), immersing the licorice powder in the bacterial liquid, fermenting at 37 DEG C for 3-14 days, taking out and drying. According to the method, the excrement is selected to be subjected to appropriate enlarged culture according to different enterotypes, the excrement inoculum which is consistent and controllable in quality is produced, and a feasible and operable production scheme is provided for standardized production of the pulvis glycyrrhizae praeparatus.

The invention provides a simulation method of chronic atrophic gastritis lesion. The simulation method comprises the following steps: (1) adopting a metamorphic lesion stage as a simulation object; (2) adopting a simulation form of spasmolysis polypeptide expression metaplasia; and (3) conditionally knocking out a GRIM-19 gene from gastric mucosa wall cells. Initialized response after gastric mucosa damage is successfully simulated, IM and even gastric cancer can be developed under continuous stimulation of chronic inflammation, simulation of pathology formation provides a basis for research on early prevention and control of intestinal gastric cancer and effective restraint of precancerous lesions of gastric cancer, a research basis can be provided for screening and development of drugs for preventing and treating CAG, and an important experimental tool is provided for anti-inflammatory and anti-cancer drug tests. The method has the characteristics of high simulation degree, stability, durability, specific phenotype and high efficiency.

The invention relates to a crystal form of an intestinal type-2B sodium phosphate synergistic transportprotein inhibitor and a preparation method thereof, in particular to a crystal form II of N<1>-methyl-N<1>-(2-morpholine ethyl)-N<3>-(3-(4-phenylethyl phenylcarbamyl)-4,5,6,7-tetrahydrobenzo[b]thiophene-2-yl)isophthalamide sulfate (a compound shown in formula (I)) and a preparation method. The crystal form II of the compound in formula (I) obtained in the invention has good chemical stability and crystal form stability and can be well applied to the clinical treatment. (Shown in the description).

The invention discloses an intestinal microecology differential bacteria classification processing method and an intestinal health assessment method, and the method comprises the following steps: S10, collecting a sample set A which at least comprises n samples composed of an unhealthy group sample DG and a control group sample CG; step S11, converting the digital feature of each sample from absolute abundance to relative abundance; step S12, dividing all samples in the sample set A into k groups of intestinal types according to the similarity of absolute abundance, marking an intestinal type set obtained after classification as AB, then separating an unhealthy group sample DG and a control group sample CG in the intestinal type set AB, marking the unhealthy group sample set as DG *, and marking the control group sample set as CG *; and S13, calculating the relative abundance difference between the unhealthy group sample set DG * and the control group sample set CG *, and sorting the relative abundance difference to obtain difference characteristics DF corresponding to the k groups of intestinal types. The invention not only can effectively extract high-dimensional sample features with a large abundance change range, but also is higher in anti-interference performance.

This invention is intended to provide a method capable of diagnosis of pancreatic tumor type at an early stage. More specifically, this invention relates to an examination method for determining pancreatic tumor type comprising detecting the degree of methylation in a 5′-untranslated region or a region comprising a 5′-untranslated region and a translated region of a gene encoding a mucin core protein in a pancreatic fluid sample from a subject, and identifying the pancreatic tumor as being of pancreatic tumor type selected from the group consisting of pancreatic ductal adenocarcinoma, an intraductal papillary mucinous neoplasm of the gastric type, an intraductal papillary mucinous neoplasm of the intestinal type, and an intraductal papillary mucinous neoplasm of the pancreatobiliary type using the degree of methylation as an index value.

The present invention relates to thienocycloalkyl or thienoheterocyclic derivatives, a preparation method thereof and use thereof in medicine. In particular, the present invention relates to thienocycloalkyl or thienoheterocyclic derivatives shown by the general formula (I), a preparation method thereof and a pharmaceutical composition containing the derivatives, and the use thereof as a therapeutic agent, especially as the intestinal type 2B sodium phosphate cotransporter (Npt2b) inhibitor and use thereof in the preparation of drugs for the treatment and/or prevention of hyperphosphatemia, wherein the definitions of the substituents in the general formula (I) are the same as defined in the description.

The invention provides a pharmaceutical composition for preventing and treating intestinal escherichia coli infection of yak calves. The pharmaceutical composition is prepared from the following components: artemisinin, antibiotics, trace element selenium, ferrous sulfate, shell powder and mannan oligosaccharide. The invention further provides a preparation method of the pharmaceutical composition and application of the pharmaceutical composition in preventing and treating intestinal colibacillosis of yak calves. The pharmaceutical composition can be used for improving the drug sensitivity of drug-resistant escherichia coli and the antibacterial effect of fluoroquinolone-or beta-lactam antibiotics and can be used for treating hemorrhagic diarrhea of yak calves caused by enterotoxigenic escherichia coli infection, and the prepared dosage form is convenient for clinical use; and meanwhile, the pharmaceutical composition can further promote the body immune function and production performance of the yak calves, enhance the resistance of the yak calves to pathogenic bacteria escherichia coli and improve the immunity of yak groups. According to the preparation method, material selection is convenient, cost is relatively low, and large-scale production is easy to form.