16 results about "Intestinal type" patented technology

The invention discloses an intestinal typing method based on a flora transplantation receptor and application of the intestinal typing method in prediction of the treatment effect of an immune checkpoint inhibitor, and the intestinal typing method comprises the following steps: carrying out DNA sequencing on an excrement sample from the flora transplantation receptor to obtain the proportion of the relative abundance value of a characteristic flora in a total flora; when the proportion of the relative abundance value of the characteristic flora 1) in the total flora is the highest, the intestinal type is judged as an ERumin intestinal type; when the ratio of the relative abundance value of the characteristic flora 2) is the highest, the intestinal type is determined to be an EEsch intestinal type; when the ratio of the relative abundance value of the characteristic flora 3) is the highest, the intestinal type is determined to be the EBact intestinal type; when the proportion of the relative abundance value of the characteristic flora 4) is the highest, the intestinal type is judged as an EClos intestinal type; and when the relative abundance value proportion of the characteristic flora 5) is the highest, determining that the intestinal type is the EPrev intestinal type. The treatment effect of the flora transplantation receptor on the immune checkpoint inhibitor can be predicted according to the intestinal type typing, and it is verified that when the intestinal type typing is an ERumin intestinal type or an EBact intestinal type, the predicted treatment effect of the immune checkpoint inhibitor is good.

The invention relates to a method for screening and identifying metabolites and florae with a human immune regulation effect, which comprises the following steps: (1) establishing immune system humanized mice with multiple intestinal types, and analyzing the change of the florae, the metabolites and the immune system through intestinal flora sequencing, serum metabolome sequencing, flow cytometry and transcriptome sequencing; (2) determining the correlation and sequence between flora related metabolites and the change of the immune system through bioinformatics analysis; and (3) confirming the regulation and control relationship between the candidate metabolites and the immune subgroups in the step (2) through biological experiments. By means of the screening method, the chemical small molecule metabolites for regulating and controlling the human immune cells in the normal physiological state can be screened on a large scale, and the chemical small molecule metabolites can be developed into immunotherapy drugs or used as cell therapy drug culture system components. The screened propionic acid can promote cytotoxicity of T cells and CAR-T and secretion of related cytokines in the aspect of function regulation of the T cells, and the metabolite 2-hydroxybutyric acid is highly positively correlated with the Treg cells and can promote generation and functions of the Treg cells.

The application discloses a domesticated total Mucor and application thereof. The application provides the domesticated total Mucor, which is preserved in the China General Microbiological Culture Collection Center on September 19, 2022, and the preservation number is CGMCC NO. 40320. The application research finds that the domesticated total Mucor can grow in an anaerobic environment at 36-38 DEG C, can be used for preventing and / or treating radioactive diseases, expands the application field of the total Mucor, and provides theoretical guidance for clinical application. Experimental data show that the domesticated total Mucor can improve the inflammation level, integrity and function of the colon and small intestine of a mouse with intestinal type radiation injury.

The application discloses a kind of non-small cell lung cancer prognosis immunity and nutrition state related intestinal microorganism marker and its application, intestinal microorganism marker specifically includes: Eubacterium hallii, Akkermansia, Lachnospira pectinosus, Lachnospira viviparous, Paraclostridium, Haemophilus influenzae.The relative abundance of intestinal flora is used with patient PNI index, and the correlation of the diversity of intestinal flora, intestinal type and specific strain and PNI is established.The relative abundance of 6 specific strains of intestinal flora is used with patient PNI index to construct the evaluation model of nutrition immunity, by using this model, the relative abundance information of 6 specific strains of new individual is input, and the result of nutrition immunity evaluation is output, without invasive examination, can repeatedly detect, and improve the nutrition immunity state of patient by the intervention of specific intestinal flora.This provides a new way for personalized medicine, helps doctor to formulate more accurate treatment strategy.

The application discloses a microorganism marker composition of an allergic population based on enterotype typing and application thereof in preparation of a reagent or kit for identifying allergic diseases. The application also discloses a kit for identifying allergic diseases. The application further discloses a method for constructing an allergic prediction model based on enterotype typing and application thereof in construction of a system for judging allergic risks of samples. The application first proposes an allergic prediction model based on enterotype stratification, classifies samples through enterotype typing, and then predicts allergic risks of the samples by using corresponding machine learning models. The application can detect allergic risks of children by a non-invasive and non-intrusive method, and only needs feces of the children. The application provides a new technical basis for future research on allergic diseases of children and a new non-invasive and non-intrusive method for detection of allergic diseases of children.

The application discloses an intestinal type scoring model for undifferentiated spondyloarthropathy and a construction method and application thereof, and belongs to the technical field of medicines.The 16s rRNA intestinal flora amplicon of 100 uSpA subjects is detected by using high-throughput second-generation sequencing.The intestinal type classification is explored, the intestinal type scoring is constructed, and the clinical characteristics of different intestinal types are researched.The research mainly includes the following four parts:(1) collecting the stool samples and peripheral blood of the uSpA subjects, and detecting the peripheral lymphocytes and cytokines by using flow cytometry (FCM) and CBA method;(2) performing intestinal flora 16s sequencing by using the lllumina high-throughput sequencing technology;(3) analyzing the 16s sequencing data by using the QIIME2 platform;(4) describing the variability of the microbiome data by using the Dirichlet multinomial mixture unsupervised clustering, classifying the intestinal flora of the uSpA, and mining the characteristics of the intestinal flora of the subjects.

Disclosed is use of Cordyceps sinensis MBF1 in the preparation of a health product or drug for preventing and / or treating radiation injury, particularly in the preparation of a health product or drug for preventing and / or treating intestinal radiation injury and / or hematopoietic radiation injury caused by ionizing radiation. Cordyceps sinensis MBF1 can significantly ameliorate symptoms caused by intestinal radiation injury, such as shortened colon length, small intestinal tissue injury, and elevated small intestinal inflammation level, ameliorate symptoms caused by hematopoietic radiation injury, such as reduced white blood cell count, reduced spleen index, and / or reduced thymus index, and can be used as a health product or drug for preventing and treating radiation injury, particularly intestinal radiation injury and / or hematopoietic radiation injury, thereby addressing the current lack of effective health products or drugs for the clinical treatment of intestinal radiation injury and / or hematopoietic radiation injury. In addition, the present disclosure provides a new idea and strategy for developing an anti-radiation injury health product or drug and demonstrates important scientific significance and clinical application value.

The invention discloses a gastric cancer peritoneal metastasis intra-operative prediction method, which comprises the following steps of: establishing a column diagram intra-operative prediction model, and assisting an operation decision through the prediction model. The model prediction factors of the column plot intra-operative prediction model comprise Lauren typing, clinical T stage, peritoneal nodule distribution area and the ratio of neutrophils to lymphocytes. According to the prediction model in the column chart operation, the scale score range of the column chart is 0-100, including a Lauren type, including an intestinal type, a mixed type and a diffuse type, a clinical T stage, including T1-3, T4a and T4b, a peritoneal nodule distribution region, including Area a, Area b and Area c, a ratio of neutrophil to lymphocyte, including less than 2.88 or greater than or equal to 2.88, a total score, the score range is 0-400, and the peritoneal metastasis probability range is 0.1-0.95.

The application discloses application of cyclosporin in treatment of intestinal radiation injury. The application discloses a new use of cyclosporin in prevention and treatment of intestinal radiation injury. Researches show that cyclosporin can effectively prolong the survival time of a mouse model of intestinal radiation injury, reduce apoptosis of intestinal crypt cells, and increase the number of intestinal crypt stem cells after radiation, and both intraperitoneal injection and oral administration have the prevention and treatment effects, indicating that the cyclosporin has great potential market and application prospect in prevention and / or treatment of intestinal radiation sickness and gastrointestinal radiation injury caused by tumor radiotherapy.

The invention relates to a method for screening and identifying metabolites and florae with a human immune regulation effect, which comprises the following steps: (1) establishing immune system humanized mice with multiple intestinal types, and analyzing the change of the florae, the metabolites and the immune system through intestinal flora sequencing, serum metabolome sequencing, flow cytometry and transcriptome sequencing; (2) determining the correlation and sequence between flora related metabolites and the change of the immune system through bioinformatics analysis; and (3) confirming the regulation and control relationship between the candidate metabolites and the immune subgroups in the step (2) through biological experiments. By means of the screening method, the chemical small molecule metabolites for regulating and controlling the human immune cells in the normal physiological state can be screened on a large scale, and the chemical small molecule metabolites can be developed into immunotherapy drugs or used as cell therapy drug culture system components. The screened propionic acid can promote cytotoxicity of T cells and CAR-T and secretion of related cytokines in the aspect of function regulation of the T cells, and the metabolite 2-hydroxybutyric acid is highly positively correlated with the Treg cells and can promote generation and functions of the Treg cells.

The present application belongs to the field of agricultural mechanization equipment, and proposes a greening auxiliary planting device based on different mountainous conditions, which comprises a hand-held device and a walking device. The walking device comprises a front wheel mechanism, a rear wheel mechanism and a bottom bridge. A pair of ground grabbing devices are arranged at the tail of the bottom bridge. An intestinal type vehicle body is arranged above the front wheel mechanism and the rear wheel mechanism. A rotary clamping device is arranged at the front end of the intestinal type vehicle body. The rotary clamping device comprises a plurality of interval distributed lower clamping openings. A tail hoop is arranged at the position close to the rear end of the intestinal type vehicle body. A group of telescopic devices are arranged on both sides of the tail hoop. The telescopic device comprises a fixed cantilever, a movable cantilever, a plurality of upper clamping pieces and a righting clamping piece. The movable cantilever is provided with a control device arranged on the hand-held device for controlling the telescopic device to stretch and retract. The present application has the advantages of reasonable design, good mobility, better mountain passability, being conducive to auxiliary planting and improving work efficiency.

The application relates to the technical field of intestinal typing, and discloses an intestinal typing method based on intestinal microorganism flora structure, which comprises the following steps: acquiring intestinal flora sequencing data; the intestinal flora sequencing data comprises intestinal flora 16s amplicon data and metagenome sequencing data; performing quality control and species annotation analysis on the intestinal flora sequencing data to obtain first relative abundance information of sample intestinal flora; screening according to the first relative abundance information and a chi-square verification method to obtain intestinal flora characteristic data; and inputting the intestinal flora characteristic data into a preset intestinal type prediction model to perform intestinal type prediction. The 16s amplicon and metagenome sequencing data are processed and analyzed to obtain intestinal flora characteristic data, and a preset intestinal type prediction model is used to perform intestinal flora typing to predict the intestinal type. Compared with a clustering method, a machine learning method can improve the efficiency and accuracy of intestinal type identification, can perform intestinal type identification on single and multiple samples, and is not limited to single-source samples.

The invention discloses a computer device, a method and a computer product for transplanting and matching intestinal flora. According to the method, the intestinal flora microecology is modeled in a GNN isograph, the intestinal flora matching method based on a healthy donor and an indication receptor is constructed on the basis of a Transform deep learning model architecture, and an analytic hierarchy process scoring algorithm is performed by combining intestinal bacteria indexes such as intestinal types and intestinal microbiome health indexes (GHMI), so that the intestinal flora microecology can be identified. The analysis accuracy of an intestinal bacteria matching model can be remarkably improved, and the method can be applied to analysis of an intestinal microbiome relationship, intestinal similarity matching, micro-ecological difference analysis in different diseases and the like.

The application discloses a targeted nutrient source mining method based on beef cattle intestinal type-host gene interaction, and particularly relates to the field of biological information data processing, and is used for solving the problems of lack of molecular mechanism verification and poor targeting of existing nutrient source development. First, based on the microbiome and transcriptome data, the nutrient-related intestinal type is divided, and the co-expression network is constructed to screen the host co-expression specific genes driven by the specific intestinal type. Then, the model of the group metabolite ligand set and the host protein receptor is established, the molecular conformation search and the Gibbs free energy calculation are performed, and the high-activity targeted effect factor is screened based on the physical affinity. Finally, the targeted effect factor is used for traversing matching and quantitative screening of natural raw material liquid chromatography-mass spectrometry data, and a targeted nutrient source recommendation list for the specific intestinal type is generated. The application combines multi-omics correlation analysis and molecular thermodynamic verification, and constructs a precise development closed loop from micro mechanism analysis to macro raw material matching.

The invention relates to the technical field of medicines, and particularly discloses application of an antioxidant alpha-lipoic acid in preparation of a medicine for improving gastritis. Chronic atrophic gastritis (CAG) is closely related to intestinal gastric cancer, wherein spasmolysis polypeptide expression metaplasia (SPEM) is a key node of pathological progress. Alpha-lipoic acid (ALA) is used for treating an SPEM mouse model, and the ALA is found to reduce lipid peroxidation and alleviate ferroptosis by activating an NRF2 antioxidant pathway, and significantly relieve SPEM symptoms and excessive generation of proinflammatory cytokines. According to the research, the protective effect of ALA on mouse SPEM is determined, and a new improvement choice is provided for improvement and regulation of SPEM. ALA, as a naturally existing antioxidant and anti-inflammatory agent, has a broad market prospect and social value in new application in protection of SPEM. The invention is expected to open up a new research direction for prevention and treatment of chronic atrophic gastritis.

Disclosed is use of Cordyceps sinensis in the preparation of a health product or drug for preventing and / or treating radiation injury, particularly in the preparation of a health product or drug for preventing and / or treating intestinal radiation injury caused by ionizing radiation. Specifically, Cordyceps sinensis can significantly ameliorate symptoms caused by intestinal radiation injury, such as weight loss, shortened colon length, elevated ROS content in blood and / or small intestine, elevated inflammation levels in blood and / or small intestine, and small intestine tissue injury.