23 results about "Host gene" patented technology

The invention relates to the technical field of biology, and discloses sgRNA for knocking out a TNIP2 gene, the nucleotide sequence is shown as SEQ ID NO: 1, blocking of the sgRNA to TNIP2 expression is verified through sanger sequencing and Western Blotting experiments, a TNIP2 knockout cell line and a control cell have no obvious morphological and growth difference, and the sgRNA can be used for knockout of the TNIP2 gene. The sgRNA can inhibit the replication ability of the H1 subtype influenza virus by regulating and controlling the expression of a host gene TNIP2, in addition, the invention further discloses application of the sgRNA, a cell strain with the TNIP2 gene knocked out and a construction method of the cell strain.

The invention discloses application of Med26 gene / protein as a target spot in preparation of a medicine for preventing and / or treating BCG infection, and belongs to the technical field of biological medicine. Aiming at the technical problems that the host defense defect is caused by excessive death of macrophages when a host is infected with BCG, the research on the mechanism of regulating and controlling the macrophages to resist BCG infection by host genes is not perfect and the like at present, a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) screening technology is utilized to discover the key effect of the Med26 gene for negatively regulating and controlling the survival of the macrophages in the BCG infection in a BCG infection model for the first time, so that the BCG infection resistance of the host is improved. And it is verified that the survival rate of the macrophage after BCG infection can be significantly improved through targeted inhibition of Med26 gene expression, and the BCG removing capacity of the macrophage is enhanced. The discovery of the invention provides a new target for research and development of drugs for preventing and / or treating BCG infection.

The invention relates to an infectious arenavirus particle that is genetically engineered to contain a genome with the ability to amplify and express its genetic information in infected cells but incapable of producing further infectious progeny particles in normal, non-genetically engineered cells.One or more of the four arenavirus open reading frames glycoprotein (GP), nucleoprotein (NP), matrix protein Z, and RNA-dependent RNA polymerase L are removed or mutated to prevent replication in normal cells, but still allow gene expression in arenavirus vector-infected cells, and foreign genes encoding an antigen or other protein of interest or nucleic acids that regulate host gene expression are expressed under the control of arenavirus promoters, internal ribosome entry sites, or under the control of regulatory elements that can be read by the viral RNA-dependent RNA polymerase, cellular RNA polymerase I, RNA polymerase II, or RNA polymerase III. The modified arenaviruses are useful as vaccines and therapeutic agents for a variety of diseases.

The invention discloses a targeted nutrient source mining method based on beef cattle intestinal type-host gene interaction, particularly relates to the field of biological information data processing, and is used for solving the problems that existing nutrient source development lacks molecular mechanism verification and is poor in targeting. The method comprises the following steps: firstly, dividing nutrition-associated intestinal types based on microbiome and transcriptome data, and constructing a co-expression network to screen host co-expression specific genes driven by the specific intestinal types; then, establishing a flora metabolite ligand set and host protein receptor model, executing molecular conformation search and Gibbs free energy calculation, and screening high-activity targeting effect factors based on physical affinity; and finally, traversal matching and quantitative screening are carried out on the natural raw material liquid chromatography-mass spectrometry data by using the targeted effect factors, and a targeted nutrition source recommendation list aiming at the specific intestine type is generated. According to the method, multi-omics correlation analysis and molecular thermodynamics verification are fused, and an accurate development closed loop from micromechanism analysis to macroscopic raw material matching is constructed.

Provided are a regulatory element capable of regulating gene expression, and a use. The sequence of the regulatory element is any one or more of sequences shown in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, and SEQ ID NO: 6. By means of innovative bidirectional symmetrical design, the regulatory element can function in two directions at the same time. The regulatory element can be applied to lentiviral vectors, can prevent enhancers / promoters carried by the vectors from affecting downstream genes and prevent uncontrolled and unintended expression caused by enhancers / promoters of host genes at insertion sites, and thus can improve the safety of vector applications.

The application discloses a construction method of a microbial-metabolite-host regulation network of summer South China calf based on Lactobacillus reuteri, and belongs to the technical field of microorganisms. The construction method comprises the following steps: collecting rumen, fecal sample and whole blood sample of the summer South China calf; obtaining microbial layer data through 16S rRNA sequencing, obtaining metabolite layer data through non-target metabolomics, and obtaining host gene / pathway layer data through whole blood transcriptomics; performing correlation analysis on multi-omics data to obtain a microbial-metabolite-host regulation correlation network. The regulation network significantly improves the growth performance of the summer South China calf, relieves diarrhea and enhances the digestive absorption function by improving the abundance of gastrointestinal beneficial bacteria, promoting the secretion of short-chain fatty acids and activating the host immune antioxidant pathway. The application realizes the precise mechanized application of probiotics by clarifying the regulation correlation among the three, and provides a mechanism-application integrated scheme for healthy breeding of calves.

The application discloses application of a host PRKCSH gene as a target point in prevention and treatment of various RNA virus infections. Experiments prove that knocking out the PRKCSH gene of a pig can significantly inhibit adhesion and invasion of Japanese B encephalitis virus to a host cell, and can also significantly inhibit replication of a classical swine fever virus, a porcine transmissible gastroenteritis virus, a Senecavirus, a porcine epidemic diarrhea virus and a porcine reproductive and respiratory syndrome virus in the host cell. In addition, knocking out the PRKCSH gene of a human can significantly inhibit replication of the Japanese B encephalitis virus in the host cell. Therefore, specific targeted editing of the PRKCSH gene or interference with protein expression of the PRKCSH gene can inhibit infection of various RNA viruses to host cells.

The invention relates to a biosynthesis gene cluster of a tropolone compound, namely isatropolones, and a bacterial strain capable of realizing directional high yield. The gene cluster comprises an isaF gene, an isaJ gene and an isaS gene; the sequence of the isaF gene is as shown in SEQ ID NO.1, the sequence of the isaJ gene is as shown in SEQ ID NO.3, and the sequence of the isaS gene is as shown in SEQ ID NO.5. The strain capable of realizing the directional high yield is (1) a strain capable of realizing the directional high yield of isatropolone A, namely a strain which takes streptomyces sp.CGMCC (China General Microbiological Culture Collection Center) No.15540 as a host and is used for blocking an isaJ gene of the host or blocking an isaS gene of the host and is also used for realizing the overexpression of isaF at the same time, and the strain capable of realizing the directional high yield of the isatropolone A is a strain capable of realizing the directional high yield of the isatropolone A. And (2) the strain of the oriented high-yield isatropolone C is a strain which takes streptomyces sp.CGMCC (China General Microbiological Culture Collection Center) No.15540 as a host and is used for overexpressing the genes of isaF and isaS, or a strain which is used for overexpressing the genes of isaJ and isaS, or a strain which is used for overexpressing the genes of isaF, isaJ and isaS, or a strain which is used for overexpressing the genes of isaF, isaJ and isaS.

The invention relates to the technical field of biomedicine, in particular to a biomarker combination for screening lung cancer patients and application of the biomarker combination. The biomarker combination provided by the invention is based on microbiome characteristics (16S rRNA spectrum) in bronchoalveolar lavage fluid, is subjected to combined analysis with host gene mutation characteristics (EGFR / G719X + S768I), can integrate multi-dimensional biological information, has higher judgment precision in the aspect of lung cancer detection, also has region and crowd pertinence, and can be used for detecting lung cancer by aiming at the characteristics of high-risk crowds. Through specific risk factors and molecular characteristics of high-risk groups (such as coal dust related gene mutation and microbial disorder, and significant difference between a lung cancer driving gene mutation spectrum and a lung microbial community structure of the high-risk groups and those of common groups), specific detection of environment exposed groups in a high-risk area (such as a coal dust area) is realized; regional detection precision and crowd adaptability are remarkably improved, and especially the early detection effect of lung cancer is good.

The invention provides a nucleic acid fragment of helicobacter pylori (HP) which is pathogenic to gastric cancer and application of the nucleic acid fragment of the helicobacter pylori (HP). In the invention, a novel gastric cancer treatment method is explored based on an HP pathogenic mechanism, a nucleic acid fragment (EC-HP-Fs) causing gastric cancer in HP is determined, and the nucleic acid fragment can participate in an HP-induced gastric cancer process through epigenetic regulation of expression of a host gene to promote the gastric cancer process, and can be used as a new target to treat gastric cancer. The invention also provides nucleic acid inhibitors for targeted modulation. The invention provides a new target for clinical treatment and detection of infectious stomach diseases caused by HP or HP.

The present application provides a method and device for determining an enrichment probe library, wherein the method includes: obtaining the genomic sequences corresponding to the microorganisms to be enriched; determining the gene fragments corresponding to the conserved regions in the genomic sequence of each microorganism to be enriched; for each gene fragment corresponding to the microorganism to be enriched, determining the base combination corresponding to the microorganism to be enriched through a sliding window; filtering all the base combinations of the microorganisms to be enriched according to preset conditions; determining the enrichment probe library according to the identification information corresponding to each microorganism to be enriched and the filtered base combinations. The present application generates an enrichment probe library by generating base combinations according to the genomic sequences of the microorganisms to be enriched, and filtering the base combinations corresponding to multiple microorganisms to be enriched, thereby solving the technical problem of designing an enrichment probe library for multiple species or multiple nucleic acid sequences, and producing the technical effect of enriching multiple species or multiple nucleic acid sequences and removing host gene interference.

The invention relates to the technical field of medical diagnosis, in particular to a method for identifying early hormonal femoral head necrosis by using a BMSCs exosome circRNA pedigree and application thereof.The method comprises the steps that firstly, BMSCs are separated from femoral bone marrow of an SONFH patient, after identification of morphology, differentiation capacity and surface markers, BMSCs exosomes are extracted, and the BMSCs exosomes are subjected to transmission electron microscope, nanoparticle tracking analysis and Westernblot verification; the method comprises the following steps: by taking BMSCs as an exosome, enriching circRNA in the exosome, constructing an SONFH patient-derived BMSCs exosome circRNA pedigree containing 664 circRNA through high-throughput sequencing and bioinformatics analysis, screening 111 differentially expressed circRNA, and defining that a host gene is enriched in disease-related signal channels such as Jak-STAT and the like; and finally, by comparing circRNA expression characteristics of an individual to be identified and the lineage, early SONFH identification is realized. According to the invention, the defect of insufficient early diagnosis sensitivity of traditional imaging is overcome, SONFH molecular marker resources are enriched, and the method can be applied to preparation of early SONFH diagnostic reagents and formulation of molecular typing and individualized treatment schemes.

The invention relates to application of an Nr3c1 gene / protein as a target spot in preparation of a medicine for preventing and / or treating BCG infection, and belongs to the technical field of biological medicines. Aiming at the technical problems that the host defense defect is caused by excessive death of macrophages when a host is infected with BCG, the research on the mechanism of regulating and controlling the macrophages to resist BCG infection by host genes is not perfect and the like at present, a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) screening technology is utilized to discover the key function of negatively regulating and controlling the survival of the macrophages in the BCG infection in a BCG infection model for the first time, so that the application of the Nr3c1 gene in the BCG infection is realized. And it is verified that the survival rate of the macrophage after BCG infection can be significantly improved through targeted inhibition of Nr3c1 gene expression, and the BCG removal capacity of the macrophage is enhanced. The discovery of the invention provides a new target for research and development of drugs for preventing and / or treating BCG infection.

The invention relates to application of a Cd302 gene / protein as a target spot in preparation of drugs for preventing and / or treating BCG infection, and belongs to the technical field of biological medicines. Aiming at the technical problems that the host defense defect is caused by excessive death of macrophages when a host is infected with BCG, the research on the mechanism of regulating and controlling the macrophages to resist BCG infection by host genes is not perfect and the like at present, a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) screening technology is utilized to discover the key function of negatively regulating and controlling the survival of the macrophages in the BCG infection in a BCG infection model for the first time, so that the application of the Cd302 gene in the BCG infection is realized. And it is verified that the survival rate of the macrophage after BCG infection can be significantly improved through targeted inhibition of Cd302 gene expression, and the BCG removal capability of the macrophage is enhanced. The discovery of the invention provides a new target for research and development of drugs for preventing and / or treating BCG infection.

The invention discloses an analysis method for automatically processing metagenome high-throughput sequencing data binning to obtain a viral genome, which comprises the following steps: acquiring second-generation sequencing original data, and performing data quality control to obtain Reads to be analyzed; a plurality of Reads are assembled into an overlapping group through overlapping of fragments, statistics is conducted on the overlapping group, and a length distribution diagram is drawn; reconstructing a viral genome based on contig binning, and generating a box after sequence clustering; constructing a three-dimensional matrix of all boxes according to the attributes of the sequence, and predicting and identifying the species category of each box; carrying out quality identification on the virus box bin, and detecting and rejecting virus host genes; counting the abundance of the virus box bin in each sample to generate a virus box bin abundance table; constructing a virus annotation database, completing species annotation, constructing a virus evolutionary tree, and finally generating a report. The invention further discloses an analysis system and application for implementing the analysis method.

The invention discloses a rapid trichina detection system based on RAA-CRISPR / Cas12a, a kit and a use method of the kit. According to the present invention, based on the RAA technology, the isothermal amplification is performed on the ribosome small subunit gene of trichina, the reaction result is visualized by using the specific and visual CRISPR / Cas12a enzyme, and the established RAA-CRISPR / Cas12a diagnostic kit can conveniently, rapidly and efficiently diagnose trichina infection. Meanwhile, the diagnosis effectiveness evaluation result of the kit in mouse muscles, blood and feces shows that the detection kit can complete rapid detection of trichina in a sample within 60 min at 37-39 DEG C, the detection sensitivity can reach 10 fg / mu L, and the kit is suitable for slaughter houses, fields and other places without special equipment. Besides, the kit can detect that except T10, T1-T12 and other trichina genotypes have no cross reaction with toxoplasma gondii and other parasites and host genes, human trichinosis can be prevented, and instant detection of related meat foods can be realized.

The invention belongs to the technical field of bioinformatics, and discloses a conjoint analysis method for pathogen recognition and patient genotype characteristics. The method comprises the following steps: firstly, carrying out metagenome sequencing on a positive clinical sample, obtaining original sequencing data, respectively carrying out quality control treatment and data classification on the original sequencing data, identifying a pathogenic pathogen DNA sequence and a host DNA sequence, then carrying out noise reduction treatment on the pathogenic pathogen DNA sequence, identifying the pathogenic pathogen and drug-resistant genes and virulence factors carried by the pathogenic pathogen, and identifying the drug-resistant genes and virulence factors carried by the pathogenic pathogen. Meanwhile, host genotype analysis is carried out on the basis of host DNA sequence characteristics, and finally composition information of pathogenic pathogens, a list of drug-resistant genes and virulence factors and host genotype variation characteristics are obtained. According to the method, a host gene variation analysis process and a conventional metagenome pathogenic pathogen detection analysis process are integrated, so that comprehensive analysis of host and pathogenic pathogen characteristics is realized, and the accuracy and efficiency of clinical diagnosis and treatment are improved.

The invention relates to an infectious arenavirus particle that is engineered to contain a genome with the ability to amplify and express its genetic information in infected cells but unable to produce further infectious progeny particles in normal, not genetically engineered cells. One or more of the four arenavirus open reading frames glycoprotein (GP), nucleoprotein (NP), matrix protein Z and RNA-dependent RNA polymerase L are removed or mutated to prevent replication in normal cells but still allowing gene expression in arenavirus vector-infected cells, and foreign genes coding for an antigen or other protein of interest or nucleic acids modulating host gene expression are expressed under control of the arenavirus promoters, internal ribosome entry sites or under control of regulatory elements that can be read by the viral RNA-dependent RNA polymerase, cellular RNA polymerase I, RNA polymerase II or RNA polymerase III. The modified arenaviruses are useful as vaccines and therapeutic agents for a variety of diseases.

The application discloses a targeted nutrient source mining method based on beef cattle intestinal type-host gene interaction, and particularly relates to the field of biological information data processing, and is used for solving the problems of lack of molecular mechanism verification and poor targeting of existing nutrient source development. First, based on the microbiome and transcriptome data, the nutrient-related intestinal type is divided, and the co-expression network is constructed to screen the host co-expression specific genes driven by the specific intestinal type. Then, the model of the group metabolite ligand set and the host protein receptor is established, the molecular conformation search and the Gibbs free energy calculation are performed, and the high-activity targeted effect factor is screened based on the physical affinity. Finally, the targeted effect factor is used for traversing matching and quantitative screening of natural raw material liquid chromatography-mass spectrometry data, and a targeted nutrient source recommendation list for the specific intestinal type is generated. The application combines multi-omics correlation analysis and molecular thermodynamic verification, and constructs a precise development closed loop from micro mechanism analysis to macro raw material matching.

The present invention aims to provide a method for rapidly, simply, and reliably identifying patients with nontuberculous mycobacterial lung disease (pulmonary NTM disease) compared to lung cancer patients, healthy controls, or chronic airway inflammatory diseases other than pulmonary NTM disease, as well as a marker to be used in this method. [Solution] According to the present invention, it is possible to provide a biomarker consisting of the SLC26A4 gene, the SLC26A4 protein, or the SLC26A4-AS1 gene for distinguishing between subjects with pulmonary NTM disease and lung cancer patients or healthy controls or chronic airway inflammatory diseases other than pulmonary NTM disease, a method of identification using the gene or protein, and a kit containing the gene.

Various embodiments are directed to analyzing host gene expression levels and microbial diversity in a biological sample, to determine a likelihood of lower-respiratory tract infection (LRTI) in subjects. Embodiments can include determining a probability value of a subject having LRTI based on differential gene expression of the subject and reference levels of control subjects. Embodiments can also include determining the likelihood of LRTI in subjects based on amicrobial diversity index or abundance levels of microbes that are considered as potential pathogens. Embodiments can also include applying an integrated classifier to gene expression levels, virus abundance levels, and microbial diversity to determine the likelihood of LRTI in subjects.

The invention discloses application of a targeted ABHD6 inhibitor in preparation of a product for resisting porcine reproductive and respiratory syndrome virus and an antiviral product thereof. The inhibitor for targeting ABHD6 is a compound WWL70 for inhibiting the activity of host ABHD6, and / or a small interfering RNA for silencing or down-regulating the expression level of a host ABHD6 gene, the small interfering RNA is selected from SI-ABHD6-2 and / or SI-ABHD6-3, and the antisense strand of SI-ABHD6-2 is as shown in SEQ ID NO: 8; and the antisense strand of the SI-ABHD6-3 is as shown in SEQ ID NO: 10. Experiments prove that the compound WWL70 and the small interfering RNA (SI-ABHD6-2 or SI-ABHD6-3) are respectively used for treating cells and then infecting the cells with the porcine reproductive and respiratory syndrome virus, so that the replication of the porcine reproductive and respiratory syndrome virus can be obviously inhibited, and the effect of resisting the infection of the porcine reproductive and respiratory syndrome virus is achieved.

The present application belongs to the field of genetic resources and breeding, and particularly relates to a screening method of related genes and microorganisms balancing growth traits and disease resistance traits. The present application finds that the microbial population in gill mucus changes significantly during the attack, and finds the microorganisms resisting the infection of pathogenic bacteria by using the finding, and further finds the genes balancing growth traits and the infection of pathogenic bacteria by combining with whole genome association analysis. The present application first discloses the role of gill mucus microorganisms and their associated host genes in balancing growth and disease resistance traits of fish, provides candidate genes for molecular marker assisted breeding of fish, and provides a new idea for improving the disease resistance of fish by microbial regulation.