9results about How to "Efficient editing" patented technology

The present application relates to a promoter pAtEF1 alpha for improving gene editing efficiency of dicotyledon plants and application thereof, and belongs to the field of plant genetic engineering.The pAtEF1 alpha promoter can be used for expression of adenine base editing protein, cytosine base editing protein, Cas nuclease and gRNA.The present application further provides an expression cassette group containing the pAtEF1 alpha promoter and a recombinant vector containing the expression cassette group.The pAtEF1 alpha promoter can be widely applied to the fields of gene editing and base editing of dicotyledon plants, and has wide application in genetic improvement of dicotyledon crops such as tomato and biosynthesis of high-value metabolites.

This application presents a gRNA probe library, its preparation and design methods, a method for targeting genes, and a nucleic acid imaging method. This application combines gRNA probes containing modified groups with nucleases, enabling dynamic observation of DNA and RNA by binding to target genomic sequences or RNA transcripts in living cells. By utilizing fluorescently labeled crRNAs, tracrRNAs, and sgRNAs, this application achieves real-time imaging of repetitive and non-repetitive sites, establishing a powerful gene imaging toolkit. Furthermore, the gRNA probe library prepared in this application will be applied to CRISPR systems for gene editing, transcriptional regulation, gene targeting, and delivery.

The application discloses a plant gene editing vector and a method and application of editing genes, and particularly provides a nucleotide sequence for encoding a CasWM protein after codon optimization, and a nucleic acid construct and a kit for gene editing. The optimized gene editing vector is introduced into plant cells, and high gene editing efficiency can be obtained, so that the plant traits can be improved, and new plant varieties can be cultivated.

The application discloses a Cas12a protein mutant, a base editor containing the same and application. The Cas12a protein mutant is based on dLbCas12a protein and is subjected to any one or a combination of at least two of the following mutations: D156R, G532R and K538R combination mutation, G532R and K595R combination mutation or G532R, K538V and Y542R combination mutation. The application is based on dLbCas12a and is reformed to obtain a Cas12a protein mutant with a widened PAM recognition range, and a high-efficiency base editor capable of recognizing a widened PAM range is constructed. The base editor can realize high-efficiency C>T or A>G editing on a classic PAM and a non-classic PAM at a cell and embryo level, can realize simultaneous multi-site high-efficiency C>T or A>G editing at a cell and embryo level, and successfully enriches an existing base editing toolbox.

The problem is to provide an information processing apparatus, an information processing method, and a program capable of easily extracting all element groups that match a specific element group from a drawing in fewer man-hours. The solution is that the information processing apparatus (10) includes a control section (11) that accepts selection of a search element group as an element group composed of at least one element used in a drawing from a user, searches for an element group that matches the selected search element group from the drawing, and displays the searched element group on a display section (15) in correspondence with a position on the drawing.

The present invention provides a printing method capable of efficiently editing the print contents of a plurality of labels. The printing method is a method performed by a printing device (100) that performs printing on a label sheet (LS) on which a plurality of labels (L) are arranged, and includes the following steps: a step (S1-S4) of editing a first label among the plurality of labels (L); and a step (S8-S10) of, when a second label operation of starting editing of a second label that is a label next to the first label exists, copying the edited contents of the first label to the second label.

The invention provides a content generation method and device, electronic equipment and a storage medium, and relates to the technical field of content creation and multimedia generation, in particular to the technical field of multi-modal content generation and editing. The method comprises the steps of obtaining a creation instruction input by a user; analyzing the creation instruction to identify a creation intention and creation materials contained in the creation instruction; processing the creation material according to the creation intention to construct a visual node graph; and if an editing instruction of a user to a specified node in the node graph is received, updating the content of the specified node according to the editing instruction, synchronizing the updated content to the specified node, and keeping the graph structure of the node graph unchanged. According to the method and the device, through accurate analysis and efficient processing of the creation instruction, the structured management and consistency maintenance of the creation content are improved, so that the continuity and consistency of the content generated in the creation process are improved, the operation complexity of a user is reduced, and the user experience is further improved.

The present application relates to the technical field of genetic engineering, and more particularly to a super mini gene editor and application thereof. The present application first performs an engineering operation of simplifying the functional domain of a TnpB editor, and systematically develops a new super mini editor of 380-400 AA. The super mini new editor of the present application can efficiently realize precise and efficient editing from mammalian cells to living animals, and has great application value.

The application belongs to the technical field of gene editing, and particularly relates to a recombinant vector, a cell and a Saccharomyces cerevisiae genome editing method based on a TIGR-Tas system. The application provides a recombinant vector, which can realize efficient multiple genome editing in a Saccharomyces cerevisiae cell through the cooperation of a TasR protein and double-spaced guide RNA. The TasR protein has a small molecular weight, a high expression efficiency in a eukaryotic cell and precise DNA cleavage activity. The double-spaced guide RNA uses double-spaced sequences to simultaneously recognize double strands of target DNA, and is completely independent of a PAM sequence, thereby breaking the bottleneck that CRISPR-Cas9 cannot be targeted due to PAM limitation. Experiments prove that the vector can realize efficient editing on multiple yeast endogenous sites (ADE2, CAN1, LYP1) without any PAM screening, has a high editing efficiency, and particularly provides a feasible editing scheme for an AT-rich genome region (such as a yeast promoter region).