16results about How to "Efficient editing" patented technology

The invention discloses a rice endogenous RNA (Ribonucleic Acid) efficient gene editing system based on a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) / Cas13b (Clustered Regularly Interspaced Short Palindromic Repeats) system and application, the rice endogenous RNA efficient gene editing system comprises a Cas13b protein subjected to sequence optimization, and the nucleotide sequence of the Cas13b protein is shown as SEQ ID NO: 1 or The system can efficiently edit a plurality of rice endogenous mRNA and long-chain non-coding RNA at the same time, has a lower incidental effect, and provides a powerful tool for rice functional genomics research and crop improvement. The CRISPR / Cas13b can target a plurality of genes at the same time, has a lower incidental effect while realizing efficient knockout, avoids the influence caused by an off-target effect to a great extent, and improves the accuracy of CRISPR / Cas13b in rice RNA editing application.

The present application relates to a promoter pAtEF1 alpha for improving gene editing efficiency of dicotyledon plants and application thereof, and belongs to the field of plant genetic engineering.The pAtEF1 alpha promoter can be used for expression of adenine base editing protein, cytosine base editing protein, Cas nuclease and gRNA.The present application further provides an expression cassette group containing the pAtEF1 alpha promoter and a recombinant vector containing the expression cassette group.The pAtEF1 alpha promoter can be widely applied to the fields of gene editing and base editing of dicotyledon plants, and has wide application in genetic improvement of dicotyledon crops such as tomato and biosynthesis of high-value metabolites.

This application presents a gRNA probe library, its preparation and design methods, a method for targeting genes, and a nucleic acid imaging method. This application combines gRNA probes containing modified groups with nucleases, enabling dynamic observation of DNA and RNA by binding to target genomic sequences or RNA transcripts in living cells. By utilizing fluorescently labeled crRNAs, tracrRNAs, and sgRNAs, this application achieves real-time imaging of repetitive and non-repetitive sites, establishing a powerful gene imaging toolkit. Furthermore, the gRNA probe library prepared in this application will be applied to CRISPR systems for gene editing, transcriptional regulation, gene targeting, and delivery.

The application discloses a plant gene editing vector and a method and application of editing genes, and particularly provides a nucleotide sequence for encoding a CasWM protein after codon optimization, and a nucleic acid construct and a kit for gene editing. The optimized gene editing vector is introduced into plant cells, and high gene editing efficiency can be obtained, so that the plant traits can be improved, and new plant varieties can be cultivated.

The application discloses a Cas12a protein mutant, a base editor containing the same and application. The Cas12a protein mutant is based on dLbCas12a protein and is subjected to any one or a combination of at least two of the following mutations: D156R, G532R and K538R combination mutation, G532R and K595R combination mutation or G532R, K538V and Y542R combination mutation. The application is based on dLbCas12a and is reformed to obtain a Cas12a protein mutant with a widened PAM recognition range, and a high-efficiency base editor capable of recognizing a widened PAM range is constructed. The base editor can realize high-efficiency C>T or A>G editing on a classic PAM and a non-classic PAM at a cell and embryo level, can realize simultaneous multi-site high-efficiency C>T or A>G editing at a cell and embryo level, and successfully enriches an existing base editing toolbox.

The invention provides sgRNA for targeted knockout of an LPA gene, a recombinant expression vector and a method for knocking out the LPA gene, and relates to the technical field of gene engineering. According to the sgRNA for targeted knockout of the LPA gene, the nucleotide sequence of the sgRNA is selected from any one of the sequences as shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 9, SEQ ID No. 11, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 14 and SEQ ID No. 15. According to the present invention, by using the characteristics of the CRISPR / Cas9 gene editing system, aiming at all exons and introns of the LPA gene, all the targets capable of gene knockout are designed, and the editing efficiency and the expression level are evaluated, such that the new target is provided for the new clinical drug in the future.

The invention relates to the technical field of gene engineering, in particular to establishment and application of a plant virus mediated ABE base editing system in cotton. According to the invention, CLCrV is used as a delivery medium, nCas9-TadA7.10-OE cotton is used as a transformation receptor, and an ABE base editing system based on CLCrV mediation is constructed. A gene expression quantity result shows that nCas9 is stably genetically expressed in different transgenic lines. Different constructed CLCrV-ABEs virus vectors are inoculated to nC as9-TadA7.10-OE cotton, mutation detection is carried out, a deep sequencing result shows that CLCrV-AtU6-26: sgGhPEBP-ABE can effectively cause conversion from an A basic group to a G basic group of a GhPEBP gene target site, and the editing efficiency is 6.18%-29.26%. Meanwhile, off-target detection is carried out in a plant subjected to base editing of GhPEBP, and the result shows that no off-target phenomenon is detected on the predicted potential off-target site, so that the CLCrV-mediated ABE base editing system has certain gene editing specificity. The establishment of the plant virus-mediated ABE base editing system in cotton provides a favorable technical tool for screening efficient sgRNAs in cotton and designing and breeding cotton molecules.

The invention discloses a page generating and updating method, and belongs to the technical field of Internet. According to the scheme, firstly, a page template file is designed, semantic tags are used as placeholders in the page template file, a corresponding HTML code template is defined for each semantic tag, and a semantic tag plug-in of a rich text editor is developed; creating an editing interface according to the page template file, creating a rich text editor for each semantic tag in the template file, and editing content corresponding to each semantic tag through the rich text editor and the semantic tag plug-in thereof; then monitoring the change of the content of the rich text editor, and binding the HTML code needing to be replaced with the semantic tag to form an HTML code snippet list; and finally, the template engine generates or updates the page according to the HTML code snippet list. By means of the template file and the rich text editor plug-in, flexible editing and efficient management of page content are achieved, and the operation efficiency is remarkably improved.

The problem is to provide an information processing apparatus, an information processing method, and a program capable of easily extracting all element groups that match a specific element group from a drawing in fewer man-hours. The solution is that the information processing apparatus (10) includes a control section (11) that accepts selection of a search element group as an element group composed of at least one element used in a drawing from a user, searches for an element group that matches the selected search element group from the drawing, and displays the searched element group on a display section (15) in correspondence with a position on the drawing.

The invention provides a video editing processing method. The video editing processing method comprises the following steps: identifying and marking required materials through a specified identification mode; the data formats according to the identification marks are arranged and compiled into record texts; selecting a data format in the compiling record text to execute editing storage; and sorting into a training text according to the existing recorded text, and executing into a finished video through a training text code. According to the video editing processing method, the purpose of artificial intelligence video production can be achieved, and automatic batch generation of one type of videos is realized, so that the video production efficiency is improved, the video production cost is reduced, and the requirement of how to automatically process basic video editing processing work is met.

The present invention provides a printing method capable of efficiently editing the print contents of a plurality of labels. The printing method is a method performed by a printing device (100) that performs printing on a label sheet (LS) on which a plurality of labels (L) are arranged, and includes the following steps: a step (S1-S4) of editing a first label among the plurality of labels (L); and a step (S8-S10) of, when a second label operation of starting editing of a second label that is a label next to the first label exists, copying the edited contents of the first label to the second label.

The present invention discloses a high-fidelity LnCBE system for realizing efficient C / G to T / A editing on the gC motif of DNA. The editing system constructs a backbone vector by engineering the segmentation protein of the DddA domain of the double-stranded DNA cytosine deaminase from Lachnospiraceae bacterium sunii NSJ‑8, which can realize more efficient on-target editing and extremely low off-target editing on DNA compared with the original version of the deaminase. At the same time, a DNA double-stranded deaminase immunosuppressive protein is disclosed, which can effectively inhibit the off-target editing of nuclear DNA in the LnCBE editing system. The LnCBE editing system can be used to prepare a gene editing kit, construct an animal model of mitochondrial DNA pathogenic mutation from C / G to T / A, and provide a new tool for gene editing technology.

The invention provides a content generation method and device, electronic equipment and a storage medium, and relates to the technical field of content creation and multimedia generation, in particular to the technical field of multi-modal content generation and editing. The method comprises the steps of obtaining a creation instruction input by a user; analyzing the creation instruction to identify a creation intention and creation materials contained in the creation instruction; processing the creation material according to the creation intention to construct a visual node graph; and if an editing instruction of a user to a specified node in the node graph is received, updating the content of the specified node according to the editing instruction, synchronizing the updated content to the specified node, and keeping the graph structure of the node graph unchanged. According to the method and the device, through accurate analysis and efficient processing of the creation instruction, the structured management and consistency maintenance of the creation content are improved, so that the continuity and consistency of the content generated in the creation process are improved, the operation complexity of a user is reduced, and the user experience is further improved.

The present application relates to the technical field of genetic engineering, and more particularly to a super mini gene editor and application thereof. The present application first performs an engineering operation of simplifying the functional domain of a TnpB editor, and systematically develops a new super mini editor of 380-400 AA. The super mini new editor of the present application can efficiently realize precise and efficient editing from mammalian cells to living animals, and has great application value.

The application belongs to the technical field of gene editing, and particularly relates to a recombinant vector, a cell and a Saccharomyces cerevisiae genome editing method based on a TIGR-Tas system. The application provides a recombinant vector, which can realize efficient multiple genome editing in a Saccharomyces cerevisiae cell through the cooperation of a TasR protein and double-spaced guide RNA. The TasR protein has a small molecular weight, a high expression efficiency in a eukaryotic cell and precise DNA cleavage activity. The double-spaced guide RNA uses double-spaced sequences to simultaneously recognize double strands of target DNA, and is completely independent of a PAM sequence, thereby breaking the bottleneck that CRISPR-Cas9 cannot be targeted due to PAM limitation. Experiments prove that the vector can realize efficient editing on multiple yeast endogenous sites (ADE2, CAN1, LYP1) without any PAM screening, has a high editing efficiency, and particularly provides a feasible editing scheme for an AT-rich genome region (such as a yeast promoter region).

The invention relates to a genome editing system and method for melilotus albiflora based on a hairy root genetic transformation system, and belongs to the technical field of plant genetic engineering. According to the invention, an editing carrier system of the MaPDS gene is constructed according to two target sites on the MaPDS gene of the sweet clover, and the editing system is CRISPR2.0-MtLAP1-KO-MaPDS; according to the editing system, sweet clover hypocotyl is used as an explant material, hairy roots are obtained through an agrobacterium rhizogenes-mediated hypocotyl explant infection method, and meanwhile, the hairy roots are obtained on the basis of the principle that anthocyanin is over-expressed and accumulated based on an MtLAP1 transcription factor for color development. Positive hairy roots are obtained through visual, rapid and efficient screening, and efficient editing of the MaPDS gene of the red positive hairy roots is achieved. The method provided by the invention can effectively reduce the occurrence of solid roots in the inducing process of the sweet clover hairy roots, prevent agrobacterium pollution, reduce the detection workload and realize efficient gene editing of target genes.