Rice thousand kernel weight gene TGW6 guided RNA target sequence and application thereof

Abstract

The invention belongs to the field of plant biotechnology, and in particular relates to rice thousand kernel weight gene TGW6 guided RNA target sequences and application thereof. The oligonucleotide sequences of the rice thousand kernel weight gene TGW6 guided RNA target sequences are as shown in SEQ ID NO.1-6. The group of the target sequences is applicable to the editing of rice genome, and has the advantages of being convenient, efficient and the like. When the rice thousand kernel weight gene TGW6 guided RNA target sequences provided by the invention are used for editing the rice genome, the mutation frequency of rice thousand kernel weight gene tgw6 is 90% or above, including more than 50% of fragment-deleted homozygous mutants and around 40% of diallelic heterozygous mutants. The invention provides an important reference for the rapid establishment of high-quality new rice germplasm having an important application value on rice quality and like production, and is expected to provide a safe and efficient new way for the innovation of rice germplasm resources; and the invention has important theoretical and practical significance.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a rice thousand-grain weight gene TGW6 guide RNA target sequence and its application. Background technique [0002] Rice (Oryza sativa L.) is one of the most important food crops in the world, feeding more than half of the world's population and the main source of food for nearly half of my country's population. With the continuous growth of population, people's demand for food is also increasing. The effective use of heterosis has played an important role in promoting the increase of rice yield. However, the rice yield per unit area has not been greatly improved, and with the reduction of cultivated land, the planting area of ​​rice has been declining. It is urgent to use new Genetic improvement strategies to greatly increase rice yield. In recent years, with several genes controlling grain size (GS3, SW5 and GW8), grain width and grain weight (GW2, GW5), grain l...

AI Technical Summary

Problems solved by technology

At present, the CRISPR / Cas9 system has successfully achieved targeted genome editing in plants such as Arabidopsis thaliana, tobacco, sweet orange, rice, wheat, sorghum, corn, and bryophytes, but it is important for the yield and quality of rice breeding. There are few reports on the targeted editing of key genes such as , fertility, etc., and there is even a lack of evaluation of the breeding value of related mutants

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