572 results about "Heterosis" patented technology

The invention discloses a gene for controlling male reproduction and development of rice and application of the gene. The gene has a nucleotide sequence represented by SEQ ID NO.1, and a protein encoded by the gene has an amino acid sequence represented by SEQ ID NO.2. By targeting a LOC_Os08g20730 gene design target by virtue of a CRISPR / Cas9 gene editing system, a receptor is a middle flower 11, and a targeted T0 generation phenotype are shown in the figures 1, 2, 3 and 4 in the description; and sequencing analysis shows that anther development abnormality occurs in a homozygously mutated plant or a biallelically mutated plant and pollen does not exist. The analysis of a homozygously mutated T1 generation shows that the separation proportion of fertile plants to sterile plants is 3 to 1. The integration shows that the gene is capable of regulating and controlling the reproduction and development of rice, once the gene in the rice is damaged, the anther development abnormality occurs, and non-pollen sterility occurs. According to the obtained cell nucleus gene for controlling the reproduction and development of the rice, an intelligent sterile line can be created by virtue of a molecular measure, and excellent parents in the production are improved into excellent sterile lines, so that the heterosis utilization is expanded.

Artificial cultivation method of apis cerana queen, first preparing artifical stylobate of apis mellifera, and choosing a group of apis mellifera queen as feeding group for larva of apis cerana queen; putting the artifical stylobate of apis mellifera into a selected feeding group of apis mellifera group, taking the artifical stylobate out after cleaned by worker bee of the apis mellifera, transfering larva of worker bee in mother apis cerana group that is in need of cultivating queen to the artifical stylobate; at last putting the artifical stylobate into generation box of the selected apis mellifera feeding group for feeding larva of apis cerana queen, after 11 to 12 days the apis cerana queen stylobate is mature, taking out the apis cerana queen stylobate before the virgin queen going out the room, introducing the virgin queen to apis cerana mating group for following mating, so as to become a spawning queen. The superiority of the invention is: obtaining large amount of mature apis cerana queen stylobate or virgin queen for one time; because of adequent apis mellifera bee milk, bigger and stronger virgin queen can be cultivated; apis mellifera and apis cerana of different species can generates nutritious heterosis which provides important meaning for apis cerana species cultivation.

The first breeding process of black-shin yellow hybrid chicken adopts pure-blood black-shin yellow chicken or synthetic black-shin yellow chicken as male parent and pure or synthetic three-yellow chicken or recessive white-feather chicken as female parent in producing the hybrid chicken. The second breeding process adopts pure-blood black-shin yellow chicken or synthetic black-shin yellow chicken as male parent and the F1 generation hybrid produced in the first process as female parent in producing the F2 generation hybrid chicken. The hybrid chicken has the outer appearance of black-shin yellow chicken as well as powerful disease resistance, fast growth speed, high egg yield, high feed converting rate and other heterosis.

The invention discloses a method for planting a mixed mangrove forest in groups. According to the method, local mangrove species are planted with row as unit; the direction of the row is parallel to the coastline; one local mangrove species is planted in each row; kandelia candel hypocotyls are inserted between the rows; the local mangrove species comprise Bruguiera gymnorrhiza, kandelia candel, Rhizophora stylosa, avicennia marina and Tung blossoms; and the mangrove species are 2-3 years old and the mangrove species in one row closest to the seat are 3 years old. The method is beneficial for bringing colony heterosis and boundary advantages of each mangrove species into full play, and has the advantages that topographies and spaces are sufficiently utilized and forest tree growth is promoted. The method has the advantages as follows: as more mature mangroves are planted in near-sea sections, the method is beneficial for protecting the kandelia candel hypocotyls and plants in the back rows and reducing impacts of tides on the mixed forest, the surrival rate of afforestation can be increased, the use of building materials is reduced, the secondary pollution is avoided and the cost is reduced.

The invention relates to a high-yield corn planting method which is simple, easy to popularize and capable of increasing yield and income. According to the high-yield corn planting method, corn is sown in 2-3 ridges, wherein one ridge is spared; one row of the corn is sown in each ridge, wherein the row spacing ranges from 13 centimeters to 15 centimeters. According to the high-yield corn planting method, the corn yield per unit area is increased through a technological innovation to exceed the yield per unit area of ridge culture corn in the world by 20-30%; since super-density-tolerance corn hybrid seeds are used, the plant type is compact, leaves charge upward, stems are tough, and roots are flourishing; corn plants are about 230-250cm high and are planted densely and reasonably; the two ridges are used for sowing, one of the ridges is spared of sowing, and the spared ridge enhances ventilation, photopermeability and photosynthesis; the marginal advantages are given full play to; colony heterosis is given play to, so that the corn yield is increased.

The invention relates to a method for breeding and feeding a cross breeding pig. The adopted technical scheme is as follows: the method comprises the steps of: (1) selecting a breed or a strain with large number and strong adaptability in the local area as a female parent and selecting the breed or the strain with high growth speed and good carcass quality as a male parent; (2) carrying out cross combination by utilizing two-way crossing and two-way rotational crossing; (3) selecting backup sows in a brood of piglets when the piglets are 28-30 days old; (4) feeding and managing nonpregnant sows and pregnant sows; and (5) strictly preventing epidemic diseases. By utilizing the method provided by the invention, the bred breeding pigs show better heteroses and good piglets with high yield, high survival and high protein can be provided for peasant culturists.

The invention provides a method of crossbreed seed producing which performs rhizocarpous regeneration by use of the cotton sterile male line and restorer line, and the steps are that: a. first year, in the region where the cotton is able to live through the winter, the cotton male sterile line and the restorer line are respectively planted, the restorer line is used as the male parent to crossbreed with the sterile line to produce the F1 crossbreed cotton seeds so as to being used for the large-area production; b. after gaining the seeds, the sterile line and the restorer line plant live through the winter in natural environment; c. next year, the sterile line and the restorer line plant through the winter are pruned and trained, subsequently, the restorer line is used as the male parent to crossbreed with the sterile line during the flowering period, again the F1 crossbreed cotton seeds are produced so as to being used for the large-area production; d. the two steps of b and c are repeated for 1 to 3 times. The invention promises one year planting and continuously gaining the crossbreed seeds for many years, the invention provides labor and time saving for crossbreed seed production with low cost; moreover, the advantages of the crossbreed can be kept for a long time and the planting output and the quality of the crossbreed cotton can be assured, which is convenient for the commercialized application and has extremely important significance in the popularization of the utilization technology of the cotton crossbreed advantages.

The invention relates to a method for cultivating cross rice. Wherein, it comprises that (1), selecting the light-temperature sensitive sterile system with low-temperature sterile or short-light low-temperature sterile; (2), selecting the sterile masculinity as gene system; (3), crossing sterile system S with sterile masculinity system S', to obtain stable sterile masculinity S / S' F1; (4), screening and recovering system R; (5), using S / S' F1 as mother type to be crossed with recover system R, to find the needed cross combination, to produce cross rice. The invention can improve the match property of sterile system and reduce produce cost.

The invention belongs to the field of heterosis application and in particular relates to a method for producing rice hybrid Guangliangyou 206. According to the invention, a new combination of three-line early rice is produced by hybridizing three-line male sterile line Guang 8S with restorer line D208, wherein the female parent Guang 8S is quoted from high-yield, high-quality and high-resistance three-line male sterile line selected and bred by teacher Yang Zhenyu; male parent restorer line D208 is introduced. The hybrid has the characteristics of high and stable yield, strong disease resistance and high biological yield.

A method for breeding blue crab families comprises the following steps: S1, collecting blue crabs bred in different populations of China to construct base groups, and conducting genetic diversity comprehensive evaluation, S2, respectively selecting parent crabs from the different populations, establishing the families according to the complete diallel cross mode to grow larvae and breed in a standardizing mode, and calculating hybrid heterosis rates, S3, utilizing the crabs with outstanding hybrid heterosis to mate and combine, constructing the families through indoor artificial directional mating to grow the larvae and breed in the standardizing mode, and twice screening stocking families, S3, over-wintering mated female crabs of parent groups bred indoors of the stocking families, S4, selecting the parent crabs with high egg-laying rates from each family in following spring to grow the larvae and breed in the standardizing mode, and utilizing the breeding method to continue to screen the families. By means of the method for breeding the blue crab families, the growth rate and the survival rate of the blue crabs are used as indexes, blue crab families breeding is conducted, family economic characters are comprehensively evaluated, according to breeding results, blue crab norms are improved in the harvesting process, the breeding survival rate is improved, so that breeding benefits are greatly increased, and deviation caused by single character breeding is avoided.

The invention belongs to the technical field of poultry genetic breeding, and particularly relates to a hybrid seed production method of high uniformity yellow chicken three-line combination suitablefor chilled marketing. According to the method, a slow-feathering, yellow-feathering and green-shank chicken line is adopted as a first male parent, a fast-feathering, yellow-feathering, yellow-shank,high-yield and high-quality chicken line is adopted as a first female parent, and hybridization is conducted to obtain an F1 female parent; a local chicken line which is fast-feathering, and yellow-feathering, jute-feathering, sexual precocity and big cockscomb in appearance with the weight within + / -10% of the average weight is selected as a terminal sire, and hybridization is conducted betweenthe terminal sire and the F1 female parent to obtain commercial generation with high uniformity. According to the method, by means of regular and molecular breeding, selections of physical conformation, sexual precocity, weight uniformity and slaughter traits and comprehensive selection of meat quality traits are focused on and carried out, the bred yellow chicken commercial generation is high inuniformity and survival rate, and significant in heterosis; and the chicken has big cockscomb, green shank, fine pores and firm skin.

The invention relates to a method for cultivating hybridized and backcrossed Argopecten product fries. The method is characterized by comprising the following steps of: selecting excellent individuals that only a female area grows in a hybridized first filial generation family or group of Argopecten irradians and Argopecten purpuratus; synchronously maturing the selected excellent individuals with excellent individuals of the Argopecten purpuratus; inducing the hybridized first filial generation individuals to ovulate by using a conventional shade drying and temperature stimulation method; simultaneously, stimulating the Argopecten purpuratus to spawn and discharge seminal fluid; filtering through 500-mesh bolting cloth to obtain the seminal fluid of the Argopecten purpuratus; fertilizing the seminal fluid of the Argopecten purpuratus with the hybridized first filial generation eggs; carrying out larva cultivation and fry intermediate cultivation according to a conventional method after hatching; and thus obtaining the hybridized and backcrossed Argopecten product fries having obvious heterosis. The hybridized and backcrossed marine Argopecten fries cultivated by using the method provided by the invention simultaneously have the characteristics of the Argopecten irradians and the Argopecten purpuratus; compared with the Argopecten purpuratus, the hybridized and backcrossed Argopecten product fries have obvious advantages on growth, individual size and temperature tolerance, therefore, the hybridized and backcrossed Argopecten product fries can be cultivated in sea areas in the north, such as Qingdao, Dalian and the like; and, compared with the Argopecten irradians, the yield at the same year is increased by more than 50%.

The invention relates to a breeding method of hybrid cabbage, in particular to a simplified breeding method for preparing hybrid cabbage by using cytoplasmic male sterile lines, which belongs to the technical field of plant culture. The invention directly adopts the cabbage varieties of Zhonggan No.22 and Hugan No.2 prepared by cytoplasmic male sterile lines sold in the market as maternal plants, and adopts cabbage inbred lines (with the same ball shape as the maternal plants, the same type of leaf ball mature performance as the maternal plants and stable botanical characters) as pollen suppliers for preparing good coenospecies. The invention has the advantages that when the advantages of using the cytoplasmic male sterile lines for preparing the cabbage coenospecies are maintained, good cabbage inbred lines do not need to be used as recurrent parents for fifth to seventh generation backcrossing and transformation with male sterilant materials, the labor is saved, the cost is saved, the breeding time is greatly shortened, and in addition, the hybrid vigour parts of the original varieties can be maintained into the new hybrid varieties.

The invention discloses a method for modifying hybrid matching strains of Sansui duck. The method includes firstly, selecting breeding female and male ducks; secondly, feeding the breeding female and male ducks; thirdly, establishing and managing a feeding area; fourthly, hybridizing; and fifthly, feeding in the laying stage. By optimized purification and hybridization, genetic stability of fine characters of the Sansui duck such as meat quality and the like is improved, and growth speed and feed reward of the Sansui duck are increased. The strains are established and are propagated, and accordingly the related contradiction between low growth speed and meat quality is overcome. Hybridization experiments are performed, hybrid vigor of the Sansui duck is taken advantage, the strains are selected and bred selectively, and breeding value of the Sansui duck is increased. By strain selection and selective breeding, feeding management condition improvement and the like, fertilizing rate andhatching rate are increased, breeding egg quality is improved while reproduction rate is increased, and disease resistance of Sansui duck is improved. The method is applicable to strain modification for Sansui duck.

The invention provides a method for constructing a mangrove forest on aquaculture pollution mud flat. Avicennia marina seedlings are planted on the low tide zone of the aquaculture sewage pollution mud flat; laguncularia racemosa seedlings and kenaelia candel seedlings are planted on the moderate tide zone to form a mixed forest; rhizophora stylosa seedlings, excoecaria agallocha seedlings and millettia pinnata seedlings are planted on the high tide zone to form a mixed forest; according to the mixed planting method of the rhizophora stylosa seedlings, the excoecaria agallocha seedlings and the millettia pinnata seedlings, a row of rhizophora stylosa, a row of excoecaria agallocha and a row of millettia pinnata are sequentially planted from the sea to the land to serve as an arrangement unit. The method is beneficial to achieving the population heterosis and boundary advantages of various mangrove varieties and promoting growth of the mangrove plants. The mangrove forest is constructed on the aquaculture pollution mud flat, the self-cleaning capacity of an aquaculture system is improved, the aquaculture disease occurrence rate is reduced by 20%, and the output of aquatic products is increased by 10% to 15%; the coastal landscape and ecological environment of the restored mangrove forest wet land attract a lot of birds to inhabit and reproduce, and compared with a traditional afforestation method, the biodiversity and ecological function are remarkably improved.

The invention discloses a method for breeding high-yield high-oil peanut species. In the method, a genetic relationship of the selected high-yield high-oil peanut species is found out by an SSR (Simple Sequence Repeat) molecule marker technology; the species with distant hybrid advantages and larger selection difference is used as a parent for preparing cross combination; the variation frequency is expanded by radiation effect, and the selecting range of a high-yield high-oil gene is expanded; the genetic linkage of an adverse gene is broken down by applying hybridization, and the recombination, polymerization and complementation of high yield, high oil and resistance are realized; an individual plant is directionally selected by using ideal characters as a target in stable generation; and the individual plant is inbred for one generation, a plant system with complete and ideal characters is further selected, and the high-yield high-oil plant system is obtained. The invention has the characteristics of rapidly breeding the high-yield high-oil peanut new species and having simple method and high operability.

The invention belongs to the field of heterosis utilization, in particular relates to a production method of hybrid rice. The hybrid rice of the invention is two-line medium-indica hybrid rice which is crossbred by matching two-line sterile-line Guangzhan 63-4s and a restorer line R96, a female parent Guangzhan 63-4s is a high-yield high-quality high-resistance two-line sterile line, and a male parent R96 is bred by a selective variant in medium indica WH26. The hybrid rice has the advantages of high yield, stable yield, strong disease resistance, high biological yield, and the like.

The invention relates to a cultivation method of the triploid salvia, the steps of the invention are that: not completely ripe good salvia seeds are collected to put into the 1 / 2MS culture medium with colchicine for mutagenesis cultivation; then the seeds are transferred into a normal 1 / 2MS culture medium for cultivation, the root tip of the seedling is fetched to perform the ploidy identification of the chromosome after the preceding process of the chromosome, and the seedling with a chromosome quantity of 2n=4x=32 is the tetraploid salvia; the tetraploid salvia is taken as a female parent and the diploid salvia is taken as a male parent to perform the crossbreed and the obtaining seed is a triploid salvia plant. The nutrient organs such as the root, stem and leaf and so on of the triploid salvia plant obviously become bigger and present remarkable crossbreed advantages, and the root medicinal material output of the triploid salvia is increased by over 30 percent. Moreover, the triploid salvia has flowers, but no seeds, thus decreasing the nutrition consumption and the crossbreed advantages of the triploid salvia can be kept over 10 years through the asexual reproduction, which enhances the disease resistance and stress resistance with strong applicability, thus being applicable to planting in every part of the country. Besides, the crossbreed advantages produced by the novel variety triploid production are able to improve the output of the salvia medicinal material planting in a large scale.

The present invention provides methods and means for determining parent inbred plant lines with good combining ability, for determining good combinations of parent inbred plant lines capable of yielding hybrid lines with high heterosis, and further for determining the agronomical performance of different plant lines, which can be performed in vitro by determining the electron flow in the mitochondria under control and stress conditions.

The invention relates to the technology of biology, mainly employing SNP (single nucleotide polymorphism) and liquid chip technologies. Muscular tissue samples are taken as research objects, and 127 SNP label loci are obtained after procedure quality control of data. A liquid chip with label upstream and downstream primers is designed and composited. Serial numbers of the 127 SNP loci range from SMS001 to SMS127. By application of 127 SNP labels, detection accuracy is improved, detection time is shortened, and detection level is improved. By the liquid chip with the SNP marked primer sequence,strong technical support and guarantee are provided for cloning of livestock genes with important economic traits, early heterosis prediction, marker assisted selection, discovery of new drugs, disease diagnosis, breeding, new-species generation, research on stress resistance and variety verification.

The invention relates to a method for planting hot pepper, which comprises that using pepper as mother and father to be crossed; using selection method to plant a better parental generation self-cross system; using pepper as mother and using pimento as father to be crossed; using selection method to plant another better parental generation self-cross system; using said two self-cross systems as parents, using hybrid technique to plant the hot pepper. The inventive hot pepper has uniform size and beautiful appearance, while the price is 0.2-0.3yuan higher than other kinds and each unit field can generate 1500kg.

The invention belongs to the technical field of utilizing heterosis of cucumber and discloses a method for breeding a new variety of cucumber. The method comprises the following processes of: selecting parent system, preparing hybrids, producing seeds of the hybrid, breeding inbred lines 9001 and 8705 of the cucumber by a system breading method taking the 9001 as a female parent and the 8705 as amale parent to prepare a hybrid seed as Dongdu No.1; and the process for preparing the hybrid seed comprises the steps of: sowing, field planting, female-inducing processing, pollinating and picking seeds. The cucumber breed bred by the method grows the cucumber by main bine, the node positions of the first female flower are 3.0 to 4.0 node, the spacing node positions of the female flowers are 3.0-5.0 node; the cucumber is long and is in a straight bar shape; the cucumber is 28-30cm long and the stem of the cucumber is 3.0cm, one cucumber is 150-200g weight, the cucumber has dark green hull, white thorn and moderate thorn knurl, the cucumber strip is straight and uniform, the cucumber has beautiful appearance and delicious tastes, can resist downy mildew, angular leaf spot, virus disease,epidemic disease, blight and whitening disease and is suitable for cultivation in spring opens.

The invention discloses a method for breeding colored rice two-line hybrid rice. The method comprises the steps of carrying out hybridizing by taking colored rice +C as a male parent and a two-line sterile line S as a female parent, and carrying out backcrossing with the female parent to obtain BCnF2 generation seeds; selecting male sterile plants, carrying out paired test cross on the male sterile plants and a two-line restoring line R, and selecting and maintaining homozygous sterile line plants with colored seed coats and corresponding hybrid seeds; detecting seed heterosis, and selecting and maintaining hybrid plants and sterile line plants with heterosis to obtain colored rice sterile line seeds; identifying the fertility, yield, quality and resistance of the sterile line to obtain excellent colored rice sterile line plants; and breeding seeds to obtain a colored rice two-line hybrid system. According to the method, the colored rice and the two-line sterile line of the approved hybrid rice are hybridized and backcrossed, and the two-line sterile line plants with the colored seed coats and heterosis are screened through the restoring line, so that the obtained colored rice hybrid rice and the sterile line thereof not only have the colored seed coats, but also have the advantages of high yield, high quality and disease resistance.

The invention discloses a wheat fertility related gene TaMS7 and an application method thereof, and belongs to the field of biotechnology. The wheat fertility related gene TaMS7 is obtained by analyzing a whole genome expression spectrum of wheat anthers in different developmental stages, and plant fertility is regulated by regulating the expression of the gene. The method is used for producing and maintaining a wheat male sterile line and preparing hybrid seeds. The gene and the method have important theoretical and practical significance for establishing efficient wheat hybrid seed production techniques, and studying wheat male sterility mechanism and heterosis.

The present invention uses rice intermedium type sterile line as female parent, uses intermedius type restoring line as male parent for cross breeding rice hybrid species. Said invention also includes that uses hsien rice sterile line and intermedius sterile line as female parent and uses rice hsien-keng crossed duplex haploid (DH) population and hsien-keng crossed recombinant inbred line (RIL) population as male parent to make test cross to obtain four hybrid populations of hybrid F1 generation. By detecting character of F1 and using hisen-keng molecule specific probe to detect keng-type index of F1 correspondent to male parent DH and RIL the F1 character-male-type keng-type index correlation can be obtained.

The invention relates to a corn breeding method capable of carrying out cold plasma treatment. The method comprises the following steps: carrying out cold plasma treatment on seeds of a core variety; carrying out generation increment breeding on the seeds subjected to cold plasma treatment, carrying out assistant breeding by molecular marking and isozyme identification, and carrying out single plant selection so as to select a selfing line with target characters and excellent variation; carrying out selfing purification and rejuvenation on the selfing line with excellent variation, and then carrying out hybridized combination to obtain good corn seeds. According to the method, the three technologies including molecular marking, isozyme identification and heterosis utilization are combined after the cold plasma treatment, so that the corn breeding period is further shortened, the breeding speed is accelerated, and a better new corn variety can be obtained by breeding; the corn breeding method and means have advancement.

The invention belongs to the field of rape breeding, in particular to a method for breeding a cabbage rape self-incompatible line. The invention aims at the problem of limiting the large area popularization of self-incompatible hybrids caused by the difficult massive propagation of the cabbage rape self-incompatible line, adopts the methods of auxiliary pollination of NaCl liquid plus honey and system selection to overcome the breeding barrier of the cabbage cole self-incompatible line, adopts the measures of early sowing, thin planting and high fertilizer and the like to culture a large-plant type cabbage rape self-incompatible line with a plurality of branches and adopts the selection methods of quality analysis and compatibility authentication and the like to obtain the protospecies of the cabbage rape self-incompatible line, thus further obtaining the protospecies of the cabbage rape self-incompatible line. The invention provides a simple and effective method for the massive propagation of the cabbage rape self-incompatible line and promotes the utilization of the advantages of the self-incompatible hybrids.

The invention relates to a fast creating method for a rice long-grain japonica type indica-compatible two-line sterile line germplasm and belongs to the technical field of crop genetic breeding. The method relates to creation of the long-grain japonica type indica-compatible rice germplasm. By taking the two-line sterile line as a sterile gene donor, the rice two-line sterile line is bred by combining techniques of anther culture, natural condition and manual climatic box fertility identification and the like, wherein the plant shape is japonica type, the grain is the long-grain japonica, the flowering time is early, the fertility is stable and the comprehensive character is excellent. The sterile line is used for massive assembly of hybrid rice new combination, so that a first generation of strong heterotic indica / japonica hybrid is expected to be screened, thereby laying a foundation for effectively utilizing advantages of rice indica / japonica hybrid.

A protospecies reproduction and origin temp purifying method for temp-sensitive sterile line of paddy rice is disclosed, which features that its selective breeding, protopecies reproduction and origin temp purifying can be conducted in the natural light temp condition. Its advantages are low cost and high safety.

The invention discloses a method for breeding inbred line by using sweet potato special materials. The method comprises a special material selection and isolation method, manpower-assisted inbreeding and inbreeding offspring testcross selection. In the invention, a sweet potato special resource which blooms and fructifies naturally and has wide compatibility is taken as a parent material, a plurality of systems with different genetic backgrounds are established at the same time, the inbreeding is performed strictly and the hybridization is avoided by taking isolation measures; the character of blooming and fructifying naturally and the target economical character are successively selected, so as to breed a inbred line material which has excellent target characters and can bloom and fructify naturally stably in the north area in China, the testcross of the inbred line materials is used for identifying the heterosis and the excellent inbred line is used for crossbreeding.