100 results about "Heterosis" patented technology

The invention relates to the field of biotechnology and plant breeding, and particularly provides a method for fixing rice heterosis by using a DMP gene. Aiming at the problems of low maturing rate and complex operation of the existing apomixis system, the haploid induction functions of the rice OsDMP8 and OsDMP13 genes are verified for the first time, the OsPAIR1, OsREC8, OsOSD1 and OsDMP genes are synchronously knocked out through a CRISPR / Cas9 technology, a'MiMe-OsDMP 'four-gene editing system is constructed, and a new thought is provided for construction of the rice apomixis system. A new solution is provided for fixing heterosis of rice apomixis, the efficiency and quality of agricultural production can be greatly improved, and the method has high economic value and wide application prospects.

The present invention relates to the fields of biotechnology and plant breeding, specifically providing a method for fixing heterosis in rice using the DMP gene. To address the low fruit set rate and complex operation issues of existing apomictic reproduction systems, the present invention verifies for the first time the haploid-inducing function of the rice genes OsDMP8 and OsDMP13. Furthermore, the CRISPR / Cas9 technology is used to simultaneously knock out the OsPAIR1, OsREC8, OsOSD1, and OsDMP genes, constructing a "MiMe-OsDMP" four-gene editing system. This system provides new ideas for constructing apomictic reproduction systems in rice and offers a new solution for fixing heterosis in rice apomictic reproduction. This system can significantly improve the efficiency and quality of agricultural production, and has significant economic value and broad application prospects.

The invention relates to the field of plant breeding, and particularly provides a method for fixing rice heterosis by using an OsZFPP gene. The method comprises the following steps: firstly, constructing an OsZFPP1 gene or OsZFPP2 gene expression cassette driven by an OsECA1 promoter; then, an expression box of rice with three target points of OsPAIR1, OsREC8 and OsOSD1 being knocked out of CRISPR / Cas9 is constructed; integrating the expression cassette into the same vector to transform hybrid rice; screening three-gene homozygous mutation and OsZFPP positive plants, and identifying diploid cloned offspring through flow cytometry and genome sequencing. According to the method, the rice OsZFPP gene is combined with MiMe to provide a novel method for fixing the heterosis of the rice through apomixis, the heterosis of the rice can be successfully fixed through the method, an apomixis system with the high maturing rate can be obtained, and a novel solution is provided for fixing the heterosis of the rice through the apomixis.

The invention discloses a corn crossbreeding intelligent matching system and method based on artificial intelligence, and the method comprises the steps: collecting molecular marker genotype data and phenotype data of candidate parents, calculating the genetic distance between the parents, and constructing a genetic distance matrix; analyzing the phenotypic complementarity degree of the parent pair, and calculating a phenotypic complementarity index; constructing a heterosis prediction model based on genetic distance and phenotype complementarity, and predicting the yield heterosis value of the candidate hybrid combination; evaluating the environmental adaptability and resistance comprehensive score of the candidate combination; and adopting a Pareto multi-objective optimization strategy to screen an optimal matching scheme. According to the method, molecular marker data and phenotype data are integrated, a heterosis prediction model considering a genetic distance nonlinear effect is constructed, collaborative optimization of multiple breeding targets such as yield, resistance and environmental adaptability is achieved, the prediction accuracy is improved by about 20% compared with that of a traditional method, and the method is suitable for large-scale popularization and application. And an intelligent and precise decision support tool is provided for corn crossbreeding.

Provided is a gene ZmFIE2 for regulating maize kernel development, an encoded protein thereof, an InDel molecular marker, and a use thereof, aiming to solve the current technical problems of low maize haploid induction rate, long breeding cycle, and low maize yield. An InDel molecular marker for detecting ZmFIE2 mutants was screened out, and a primer for InDel molecular marker was designed, the InDel molecular marker and the primer thereof can be applied to the breeding of maize kernel size varieties and maize haploid or diploid lines. From the epigenetic level, it was found that ZmFIE2 regulates the development of maize embryo and endosperm, affects the accumulation of kernel storage materials, and affects the cell cycle process of endosperm cells, which has great value in improving maize yield. By using multiple created ZmFIE2 allele mutants, research has found that the loss of ZmFIE2 function can directly determine the division of endosperm cells and apomixis, which will provide new gene resources and new ways to achieve high-frequency haploid induction and heterosis fixation in maize.

The invention relates to a male gamete killer gene S19g-A1 of a rice interspecific hybrid sterile S19 seat and application of the male gamete killer gene S19g-A1. The invention provides a rice hybrid sterility related protein S19g-A1. The amino acid sequence of the rice hybrid sterility related protein S19g-A1 is as shown in SEQ ID NO. 2. According to the invention, a key gene S19g-A1 of a rice interspecific hybrid sterile S19 site is researched and identified, function knockout is carried out on African rice S19g-A1, and pollen fertility of a mutant hybrid F1 obtained by carrying out distant hybridization on an obtained African rice s19g-a1 mutant returns to normal. Based on a gene editing technology, the invention develops a method for rapidly creating a hybrid affinity line capable of overcoming sterility of African rice hybrid, effectively eliminates S19 locus mediated interspecific hybrid reproductive disorder, breaks through the bottleneck of rice interspecific hybrid advantage utilization, provides target gene resources for distant hybrid advantage utilization and crop yield improvement, and has a wide application prospect. Good application prospects are realized.

The invention relates to a GhDR gene capable of simultaneously regulating and controlling red leaf, dwarf and cold resistance characters of cotton as well as an encoding protein and application of the GhDR gene, and relates to the technical field of plant genetic engineering, the nucleotide sequence of the GhDR gene is shown as SEQ ID NO.1, and the amino acid sequence of the encoding protein is shown as SEQ ID NO.2. According to the invention, phenotypes of high anthocyanin accumulation content in stems and leaves, dwarf plant height and low temperature resistance in a seedling stage of a cotton plant over-expressed with the GhDR gene are found, which indicates that the GhDR gene has the functions of simultaneously regulating and controlling the cotton plant height, anthocyanin accumulation and low temperature resistance in the seedling stage; the GhDR gene and the encoding protein thereof are used for regulating and controlling anthocyanin accumulation, plant height dwarfing and low-temperature stress resistance functions, so that the plant height of cotton is improved, and the cotton is suitable for mechanized production; creating cotton marker characters, and carrying out cotton heterosis utilization hybrid identification; and gene resources and improvement strategies are provided for the application in the aspects of cultivating low-temperature-resistant varieties, prolonging the growth period of the cotton by timely early sowing and the like.

The invention discloses a KASP molecular marker for identifying a Chinese cabbage nuclear male sterility gene and application of the KASP molecular marker. The invention belongs to the field of molecular biology, and particularly relates to a KASP molecular marker for identifying a Chinese cabbage nuclear male sterility gene and application of the KASP molecular marker. The method for identifying or assisting in identifying the Chinese cabbage fertility comprises the steps that the genotype of an SNP site in a Chinese cabbage genome to be detected is detected, the Chinese cabbage fertility is identified or assisting in identifying according to the genotype, the SNP site is an SNP site on a Chinese cabbage A07 chromosome, the nucleotide type of the SNP site is C or T, and the SNP site is the 1935th nucleotide of SEQ ID No: 4 in a sequence table. The SNP locus related marker can be used for Chinese cabbage male sterility molecular marker assisted breeding, and a new gene resource is provided for Chinese cabbage heterosis utilization.

The invention discloses a seed production method of a high-yield laying duck auto-sexing commercial line. The accuracy of the method is close to 100% and is obviously higher than that of artificial anal turning for male and female identification; the speed is high, and the production efficiency can be improved by 5-10 times; the labor intensity is low, and fatigue of hands, eyes and the like is avoided; the technical requirement is low; and disease transmission is avoided. According to the seed production method provided by the invention, the low heritability character heterosis is fully utilized, the egg laying performance is high, and the egg yield of 66 weeks old is close to 300; eggshells are green shells and accord with consumption preferences of Chinese people; the feather color of the adult female duck is light brown yellow, slaughtering and processing are facilitated, and the value of the obsolete duck is improved; the mode is suitable for large-scale popularization and application.

The invention discloses a corn genome breeding 45K chip and application thereof, the chip covers 44, 637 SNP loci and 27 InDel loci, and the SNP loci are uniformly distributed on 10 chromosomes and part of scaffold of a corn genome and have rich polymorphism. Compared with an existing corn chip, the SNP marker of the 45K chip is derived from re-sequencing results of 507 corn varieties, has better variety representativeness, and contains 925 key functional gene loci for close planting and high yield and 96 corn variety authenticity identification loci; the method can be more effectively used for functional gene identification, genetic diversity analysis, heterosis group division, whole genome selection analysis and variety authenticity identification.

The application discloses a kind of based on molecular marker clustering analysis's cotton hybrid breeding combination screening method, the screening method includes: step S1: using multi-traits related molecular marker to the gene typing experiment of test cotton variety, data collection and arrangement are carried out;Step S2: excellent allele frequency calculation and feature matrix construction;Step S3: data standardization and principal component analysis dimension reduction;Step S4: optimal cluster number selection and genetic clustering;Step S5: each population character analysis;Step S6: heterosis potential score and combination screening.The technical problem that present cotton breeding parent selection lacks multi-traits comprehensive quantitative evaluation is solved, and the data of breeding decision-making and intelligentization are realized.

The application belongs to the technical field of biological information, and particularly relates to a method for locating ancestral haplotype genes for deep hybrid lines. In the application, the ancestral genotype of a sample to be analyzed is obtained, and then a genomic segment significantly associated with a phenotype is obtained. Then, whole genome analysis is performed. Compared with the prior art, the method for locating ancestral haplotype genes for deep hybrid lines can effectively reduce false positives, and can also be used for heterosis analysis, capture of dominant and epistatic effects between genetic markers and the like.

Provided are a maize SNP chip and the use thereof. The SNP chip comprises a combination of immobilized probes respectively targeting markers SNP1 to SNP5061, which combination of probes comprise probes as shown in SEQ ID NOs: 1 to 5061. The SNP chip contains SNP loci specific to maize, and has a higher genome coverage rate and lower locus deletion rate. Furthermore, the addition of some loci related to important agronomic traits of maize and division of maize heterotic groups makes the chip very suitable for analysis of germplasm resource fingerprints, division of heterotic groups, and molecular-marker-assisted selection in molecular breeding.

The invention discloses a method for creating a new bolting-resistant radish germplasm by using a microspore culture technology, and belongs to the technical field of radish breeding. The method comprises the following steps: hybridizing a radish material R-16 and a bolting-resistant radish material LR-75, carrying out microspore culture, embryoid induction and regeneration on hybrid offspring plants to obtain double haplobionts, carrying out selfing, and carrying out character evaluation and screening on selfing offspring plants to obtain bolting-resistant double haplobionts with stable characters. Experimental results show that by selecting the parent material and optimizing the hormone concentration of the induction culture medium, the embryo emergence rate of the radish material is remarkably increased, and the homozygous bolting-resistant radish germplasm can be efficiently and quickly obtained. According to the method, a rich material foundation is laid for heterosis breeding of the bolting-resistant radishes, the breeding procedure is simplified, the breeding cost is reduced, the breeding process of new varieties of the bolting-resistant radishes is accelerated, and the method has great economic significance and wide application prospects.

The invention relates to the technical field of molecular plant breeding, and particularly discloses a corn DH breeding chip and application, the genetic typing of the corn DH breeding chip comprises 11,001 SNP sites, 36 InDel sites and 4 transgenic event markers; the physical positions of the 11,001 SNP loci and the 36 InDel loci are determined based on the comparison of the whole genome sequence of the corn reference genome B73v4; wherein 11,001 SNP loci are as shown in a table 1; 36 InDel sites are shown in a table 2, and information of 4 transgenic event markers is shown in a table 3. The corn DH breeding liquid chip can be used for performing DH line identification, DH heterosis group division and herbicide resistance, insect resistance and stress resistance gene identification on corn DH.

The invention provides a breed selection method for selecting chicken combining ability based on a family heterosis rate, and belongs to the technical field of breeding, the breed selection method comprises the following steps: constructing a pure line subculture group, a progeny determination group and a combining ability determination group by using a commercial line variety with more than two lines; constructing a family heterosis rate by using the average value of the sum of the descendant measured average weight of the combining ability measuring paternal line and the descendant measured average weight of the combining ability measuring maternal line and the average weight of the combining ability measuring group, and selecting seeds according to the family heterosis rate. The invention provides a determination scheme for determining the combining ability of the pure-line individuals, creates a method for quantifying the combining ability and evaluation indexes, establishes a new method for improving the combining ability of the pure-line chicken flocks through breeding, eliminates the influence of the self weight difference of the pure-line individuals on a combining ability determination result, and enables the combining ability of the pure-line groups to be compared in the same dimension.

The present invention discloses an application of a rice THION22 small peptide protein in breaking the reproductive isolation between indica and japonica subspecies of rice, belonging to the field of biotechnology. The present invention breaks the pre-zygotic reproductive isolation of the indica and japonica subspecies of rice by applying the rice THION22 small peptide protein in vitro during the indica-japonica hybridization process. The application includes the rice THION22 small peptide protein promoting the growth of japonica rice pollen tubes in the pistil of the indica rice variety, and the rice THION22 small peptide promoting the acquisition of indica-japonica hybrid F1 hybrid seeds. The present invention effectively promotes the hybridization of japonica rice pollen with some indica rice varieties, promotes the growth of japonica rice pollen tubes, produces more F1 hybrid seeds, improves the efficiency of indica-japonica hybridization breeding, and has important application value for rice hybridization breeding and heterosis practice.

The invention relates to the field of agricultural breeding, and discloses a breeding method of an ultra-short corn variety, which comprises the following steps: S0, mutagenizing a corn inbred line B73 through EMS; the method comprises the following steps: S1, selecting a corn inbred line with a dwarf character as a dwarf donor; s2, selecting a corn backbone inbred line with high yield, high quality and strong disease resistance as an improved receptor; s3, carrying out artificial hybridization on the low-source donor and the improved receptor; s4, carrying out selfing on the generation S0; s5, stabilizing the agronomic yield character; and S6, screening out the ultra-short corn inbred line with the plant height stabilized at 80-100 cm. F1, improving male and female parents with heterosis according to the operation method, and hybridizing and assembling the male and female parents. According to the method, the maize inbred line B73 is subjected to mutagenesis treatment through EMS, then multi-generation inbreeding and screening are carried out, the inbred line is obtained, and compared with a traditional natural variation breeding method, the genetic stability and variation probability of the dwarf character are improved.

The application provides a rape seedling stage hetero-plant positioning method and a hetero-plant removing method, wherein the rape seedling stage hetero-plant positioning method comprises the following steps: obtaining an image containing a plurality of seedling stage plants in a plot; identifying all the seedling stage plants in the image to obtain plant pictures, and configuring index information for each plant picture; extracting features of each plant picture based on high-dimensional features of a preset dimension to obtain a feature vector; calculating the Euclidean distance between any two feature vectors to determine the outlier index of each feature vector; presetting an outlier index threshold to screen plant pictures corresponding to feature vectors whose outlier indexes exceed the preset outlier index threshold; and determining the position information of the screened plant pictures according to the index information to obtain the position information of the hetero-plant. The application is used to solve the problem that in the prior art, a large amount of variety and feature data is required in the database, and it is still difficult to achieve high-precision hetero-plant identification.

The invention discloses a method for improving whole genome selection predictability and breeding application, and belongs to the field of rice molecular breeding. The method comprises the following specific steps: 1) performing gene sequencing and variation detection on a rice parent to obtain SNP variation information; 2) performing heterosis group division based on SNP variation of the whole genome; 3) constructing a training group and planting; 4) constructing a genome selection model and predicting the yield of the hybrid combination; according to the method, a hybrid rice yield whole genome selection model is finally constructed based on group division, training group matching and high-quality reference genome assembling of core indica rice varieties; according to the method, excellent hybrid rice is matched according to the hybrid rice yield whole genome selection model; the genome prediction model provided by the invention can be used for improving the selection efficiency of excellent hybrid combinations and accelerating the breeding process, and has important application value.

The invention relates to the technical field of biological breeding, in particular to a method for predicting corn panicle weight heterosis by using the number of heterosis effect sites, which comprises the following steps: (1) obtaining the number of significant forward panicle weight heterosis effect sites in each hybrid by using a corn hybrid population and molecular markers; (2) respectively constructing a significant forward panicle weight heterosis effect site number prediction model and a panicle weight heterosis prediction model by using the corn hybrid population; (3) predicting the number of the significant forward panicle weight heterosis effect sites of the hybrid seeds to be detected by using the constructed significant forward panicle weight heterosis effect site number prediction model; and (4) performing panicle weight heterosis prediction on the to-be-detected hybrid seeds by using the predicted number of the significant positive panicle weight heterosis effect sites of the to-be-detected hybrid seeds and the established panicle weight heterosis prediction model.

The invention relates to the technical field of biological breeding, in particular to a method for predicting corn panicle weight heterosis by using a heterosis effect, which comprises the following steps: 1) identifying corn panicle weight heterosis genetic loci by using a corn hybrid population genome molecular marker; 2) integrating the heterosis site genetic effect with significant statistics into a site heterosis effect; 3) calculating the sum of the heterosis effects of the significant forward sites in each hybrid; 4) constructing a model for predicting the total heterosis effect of the significant positive sites of the hybrid seeds; 5) performing linear fitting on the sum of the obvious positive site heterosis effects of the hybrid seeds and the panicle weight heterosis, and establishing an incidence relation; (6) predicting the sum of heterosis effects of the forward sites of the to-be-detected hybrids; and (7) predicting the panicle weight heterosis of the to-be-tested hybrid seeds through the established fitting relation by utilizing the predicted sum of the heterosis effects of the forward sites.

The present invention discloses a low-cost rapeseed hybrid seed production method, which relates to the field of utilizing hybrid vigor in Brassica napus. The method comprises: (1) hybridizing an Ogura CMS sterile line as the female parent with a plant containing a sulfonylurea herbicide resistance locus to obtain an Ogura CMS sterile line containing the sulfonylurea herbicide resistance locus; (2) planting the Ogura CMS sterile line containing the sulfonylurea herbicide resistance locus as the female parent and an Ogura CMS restorer line as the male parent in a 5:1 ratio in a seed production field, employing conventional field management methods, and harvesting the Brassica napus seeds when mature; and (3) planting the harvested seeds in a conventional field and spraying the sulfonylurea herbicide at the seedling stage to obtain Brassica napus hybrid seeds of higher purity. The present invention mixes the improved Ogura CMS sterile line with the female sterile restorer line in a seed production field to obtain the first generation of offspring, and sprays herbicides at the seedling stage to obtain rapeseed hybrids with higher purity. This method can greatly reduce the cost of seed production. The present invention also provides an effective method for the reproduction of the restorer line 9538R.

The invention provides a breeding method of a triploid flowering Chinese cabbage variety, which comprises the following steps of: (1) hybridizing cabbage type rape serving as a female parent and flowering Chinese cabbage serving as a male parent to obtain a triploid hybrid F1 generation; (2) performing phenotype identification on the triploid hybrid F1 generation, and screening combinations which are early in bolting, synchronous in main stem and lateral branch development, obvious in subgenome heterosis and resistant to clubroot; (3) performing a multi-point planting test on the excellent triploid hybrid F1 to verify the yield and disease resistance of the flowering Chinese cabbage; and (4) breeding a special triploid flowering Chinese cabbage variety for seed production. The triploid flowering Chinese cabbage cultivated by the method has the following outstanding advantages of high yield, high economic value, strong disease resistance, multi-round picking and long market supply period. The method provided by the invention solves the problems of lack of special varieties of traditional brassica campestris and low yield of brassica campestris of a dual-purpose variety of brassica campestris, provides a new technical route for germplasm innovation of brassicaceous vegetables, and has important popularization and application values.

The invention provides a molecular marker linked with a major QTL (Quantitative Trait Loci) of the total grain number of a single rice plant and application of the molecular marker. The molecular marker is TGN5. A new major QTL TGN5 for controlling the total grain number of a single plant of the super rice Y900 is identified, is positioned in a section of about 1.46 Mb of a long arm of a chromosome 5, and just falls in a hot spot region Chr5: 17.9-27.3 Mb range of indica-japonica genetic differentiation of parents; the TGN5 is reduced to an interval of about 251.39 Kb by constructing a local genetic linkage map, and 13.3% of phenotypic variation is explained to the maximum extent; the loci are verified in segregation populations of the super rice Y1 and Y2 at the same time, and the single plant total grain number heterosis of the three-stage super rice can be differentially influenced. The molecular marker provided by the invention can be applied to regulation and control of the total grain number of a single rice plant.

The invention relates to the technical field of agricultural biology, in particular to a related protein TaABCG7-8 for regulating anther and pollen development as well as a gene and application of the related protein TaABCG7-8. The amino acid sequence of the related protein for regulating and controlling plant anther and pollen development is shown as SEQ ID NO: 1. A wheat photo-thermo-sensitive male sterile line BS366 is used as an experimental material, the related gene TaABCG7-8 for regulating and controlling anther and pollen development is obtained and is subjected to gene silencing in wheat, and the result shows that anther cracking is abnormal and pollen development is abnormal. The related protein for regulating anther and pollen development has very important theoretical and practical significance on wheat reproductive development and promotion of a wheat heterosis utilization process.

The invention discloses a training group efficient construction method for waxy corn whole genome selective breeding. The method comprises the following steps: widely collecting common inbred lines or DH lines or inbred lines or DH lines subjected to genetic improvement in waxy corn breeding; carrying out phylogenetic tree, population structure and pedigree analysis on the collected waxy corn inbred line or DH line; dividing the inbred line or the DH line into a plurality of heterosis groups based on a phylogenetic tree, pedigree information and a group structure analysis result; respectively selecting a plurality of representative inbred lines from the heterosis groups; preparing a plurality of hybrids from a plurality of representative inbred lines or DH lines by adopting a sparse part dicolumn hybridization design method among different heterosis groups; performing multi-environment planting identification on the plurality of hybrids to obtain a plurality of character data; predicting phenotypes of all possible hybrids assembled by a plurality of representative inbred lines; the general combining ability effect of each representative inbred line or DH line is estimated through the predicted phenotype of the hybrid, and parent inbred line materials with high general combining ability are screened out. The method solves the problem that the predictability of newly introduced germplasm or germplasm with relatively far genetic relationship is reduced due to the fact that a training group used in the existing waxy corn whole genome selective breeding only contains a hybrid combination of a few backbone inbred lines and lacks representativeness.

The invention relates to the technical field of breeding, in particular to a method for breeding a sterile line of japonica rice with wide affinity, high exposure and early blossoming. According to the method, through combination of multi-parent hybridization and multiple cross with marker-assisted selection and phenotype tracking, accurate polymerization of multiple characters such as wide affinity, high exposure and early blossoming is realized, the breeding efficiency is high, the bred 'Ning46A' is high in seed production suitability, can be matched with an indica-japonica restorer line, fully utilizes the heterosis between subspecies, and has important production and application values.

The present application relates to the field of plant breeding, and specifically provides a method for fixing hybrid vigor of rice by using OsZFPP gene. First, an expression cassette of OsECA1 promoter driven OsZFPP1 gene or OsZFPP2 gene is constructed; then, an expression cassette of three target CRISPR / Cas9 knockout of rice OsPAIR1, OsREC8 and OsOSD1 is constructed; the above expression cassettes are integrated into the same vector to transform hybrid rice; three-gene homozygous mutant and OsZFPP positive plants are screened, and diploid clone offspring is identified by flow cytometry and genome sequencing. The method provides a new method for fixing hybrid vigor of apomixis of rice by combining rice OsZFPP gene with MiMe, which can successfully fix hybrid vigor of rice, can obtain high seed setting rate apomixis system, and provides a new solution for fixing hybrid vigor of rice apomixis.

The invention belongs to the technical field of livestock breeding, and particularly relates to a rapid breeding method for a new variety of medium-sized golden Luhua commercial chickens. The breeding method comprises the following steps: hybridizing a first female parent and a first male parent to obtain an F1-generation group; selecting F1-generation female individuals as backcross female parents, and carrying out grading hybridization on the backcross female parents and the first male parents or chickens of the same species to obtain F2-generation groups; and selecting the F2-generation male individual as a terminal male parent, and carrying out three-way hybridization on the terminal male parent and the second female parent to obtain commercial-generation chickens. According to the breeding method, through the combination of grading hybridization and three-way hybridization, the excellent quality characters are consolidated firstly, then the three-way hybridization is used for stimulating the heterosis, the bred commercial chickens have the advantages of being high in growth speed, moderate in body type, delicious in meat quality and the like, and the breeding method is clear in breeding path and high in commercial value. In addition, the breeding method effectively solves the technical problem that the existing chicken breeds are difficult to consider a plurality of excellent characters such as excellent meat quality and rapid growth.