63 results about "Heterosis" patented technology

The invention discloses a corn crossbreeding intelligent matching system and method based on artificial intelligence, and the method comprises the steps: collecting molecular marker genotype data and phenotype data of candidate parents, calculating the genetic distance between the parents, and constructing a genetic distance matrix; analyzing the phenotypic complementarity degree of the parent pair, and calculating a phenotypic complementarity index; constructing a heterosis prediction model based on genetic distance and phenotype complementarity, and predicting the yield heterosis value of the candidate hybrid combination; evaluating the environmental adaptability and resistance comprehensive score of the candidate combination; and adopting a Pareto multi-objective optimization strategy to screen an optimal matching scheme. According to the method, molecular marker data and phenotype data are integrated, a heterosis prediction model considering a genetic distance nonlinear effect is constructed, collaborative optimization of multiple breeding targets such as yield, resistance and environmental adaptability is achieved, the prediction accuracy is improved by about 20% compared with that of a traditional method, and the method is suitable for large-scale popularization and application. And an intelligent and precise decision support tool is provided for corn crossbreeding.

The invention relates to a male gamete killer gene S19g-A1 of a rice interspecific hybrid sterile S19 seat and application of the male gamete killer gene S19g-A1. The invention provides a rice hybrid sterility related protein S19g-A1. The amino acid sequence of the rice hybrid sterility related protein S19g-A1 is as shown in SEQ ID NO. 2. According to the invention, a key gene S19g-A1 of a rice interspecific hybrid sterile S19 site is researched and identified, function knockout is carried out on African rice S19g-A1, and pollen fertility of a mutant hybrid F1 obtained by carrying out distant hybridization on an obtained African rice s19g-a1 mutant returns to normal. Based on a gene editing technology, the invention develops a method for rapidly creating a hybrid affinity line capable of overcoming sterility of African rice hybrid, effectively eliminates S19 locus mediated interspecific hybrid reproductive disorder, breaks through the bottleneck of rice interspecific hybrid advantage utilization, provides target gene resources for distant hybrid advantage utilization and crop yield improvement, and has a wide application prospect. Good application prospects are realized.

The invention discloses a KASP molecular marker for identifying a Chinese cabbage nuclear male sterility gene and application of the KASP molecular marker. The invention belongs to the field of molecular biology, and particularly relates to a KASP molecular marker for identifying a Chinese cabbage nuclear male sterility gene and application of the KASP molecular marker. The method for identifying or assisting in identifying the Chinese cabbage fertility comprises the steps that the genotype of an SNP site in a Chinese cabbage genome to be detected is detected, the Chinese cabbage fertility is identified or assisting in identifying according to the genotype, the SNP site is an SNP site on a Chinese cabbage A07 chromosome, the nucleotide type of the SNP site is C or T, and the SNP site is the 1935th nucleotide of SEQ ID No: 4 in a sequence table. The SNP locus related marker can be used for Chinese cabbage male sterility molecular marker assisted breeding, and a new gene resource is provided for Chinese cabbage heterosis utilization.

The invention discloses a corn genome breeding 45K chip and application thereof, the chip covers 44, 637 SNP loci and 27 InDel loci, and the SNP loci are uniformly distributed on 10 chromosomes and part of scaffold of a corn genome and have rich polymorphism. Compared with an existing corn chip, the SNP marker of the 45K chip is derived from re-sequencing results of 507 corn varieties, has better variety representativeness, and contains 925 key functional gene loci for close planting and high yield and 96 corn variety authenticity identification loci; the method can be more effectively used for functional gene identification, genetic diversity analysis, heterosis group division, whole genome selection analysis and variety authenticity identification.

The application discloses a kind of based on molecular marker clustering analysis's cotton hybrid breeding combination screening method, the screening method includes: step S1: using multi-traits related molecular marker to the gene typing experiment of test cotton variety, data collection and arrangement are carried out;Step S2: excellent allele frequency calculation and feature matrix construction;Step S3: data standardization and principal component analysis dimension reduction;Step S4: optimal cluster number selection and genetic clustering;Step S5: each population character analysis;Step S6: heterosis potential score and combination screening.The technical problem that present cotton breeding parent selection lacks multi-traits comprehensive quantitative evaluation is solved, and the data of breeding decision-making and intelligentization are realized.

The application belongs to the technical field of biological information, and particularly relates to a method for locating ancestral haplotype genes for deep hybrid lines. In the application, the ancestral genotype of a sample to be analyzed is obtained, and then a genomic segment significantly associated with a phenotype is obtained. Then, whole genome analysis is performed. Compared with the prior art, the method for locating ancestral haplotype genes for deep hybrid lines can effectively reduce false positives, and can also be used for heterosis analysis, capture of dominant and epistatic effects between genetic markers and the like.

The invention relates to the technical field of molecular plant breeding, and particularly discloses a corn DH breeding chip and application, the genetic typing of the corn DH breeding chip comprises 11,001 SNP sites, 36 InDel sites and 4 transgenic event markers; the physical positions of the 11,001 SNP loci and the 36 InDel loci are determined based on the comparison of the whole genome sequence of the corn reference genome B73v4; wherein 11,001 SNP loci are as shown in a table 1; 36 InDel sites are shown in a table 2, and information of 4 transgenic event markers is shown in a table 3. The corn DH breeding liquid chip can be used for performing DH line identification, DH heterosis group division and herbicide resistance, insect resistance and stress resistance gene identification on corn DH.

The application provides a rape seedling stage hetero-plant positioning method and a hetero-plant removing method, wherein the rape seedling stage hetero-plant positioning method comprises the following steps: obtaining an image containing a plurality of seedling stage plants in a plot; identifying all the seedling stage plants in the image to obtain plant pictures, and configuring index information for each plant picture; extracting features of each plant picture based on high-dimensional features of a preset dimension to obtain a feature vector; calculating the Euclidean distance between any two feature vectors to determine the outlier index of each feature vector; presetting an outlier index threshold to screen plant pictures corresponding to feature vectors whose outlier indexes exceed the preset outlier index threshold; and determining the position information of the screened plant pictures according to the index information to obtain the position information of the hetero-plant. The application is used to solve the problem that in the prior art, a large amount of variety and feature data is required in the database, and it is still difficult to achieve high-precision hetero-plant identification.

The invention provides a breeding method of a triploid flowering Chinese cabbage variety, which comprises the following steps of: (1) hybridizing cabbage type rape serving as a female parent and flowering Chinese cabbage serving as a male parent to obtain a triploid hybrid F1 generation; (2) performing phenotype identification on the triploid hybrid F1 generation, and screening combinations which are early in bolting, synchronous in main stem and lateral branch development, obvious in subgenome heterosis and resistant to clubroot; (3) performing a multi-point planting test on the excellent triploid hybrid F1 to verify the yield and disease resistance of the flowering Chinese cabbage; and (4) breeding a special triploid flowering Chinese cabbage variety for seed production. The triploid flowering Chinese cabbage cultivated by the method has the following outstanding advantages of high yield, high economic value, strong disease resistance, multi-round picking and long market supply period. The method provided by the invention solves the problems of lack of special varieties of traditional brassica campestris and low yield of brassica campestris of a dual-purpose variety of brassica campestris, provides a new technical route for germplasm innovation of brassicaceous vegetables, and has important popularization and application values.

The invention provides a molecular marker linked with a major QTL (Quantitative Trait Loci) of the total grain number of a single rice plant and application of the molecular marker. The molecular marker is TGN5. A new major QTL TGN5 for controlling the total grain number of a single plant of the super rice Y900 is identified, is positioned in a section of about 1.46 Mb of a long arm of a chromosome 5, and just falls in a hot spot region Chr5: 17.9-27.3 Mb range of indica-japonica genetic differentiation of parents; the TGN5 is reduced to an interval of about 251.39 Kb by constructing a local genetic linkage map, and 13.3% of phenotypic variation is explained to the maximum extent; the loci are verified in segregation populations of the super rice Y1 and Y2 at the same time, and the single plant total grain number heterosis of the three-stage super rice can be differentially influenced. The molecular marker provided by the invention can be applied to regulation and control of the total grain number of a single rice plant.

The invention relates to the technical field of agricultural biology, in particular to a related protein TaABCG7-8 for regulating anther and pollen development as well as a gene and application of the related protein TaABCG7-8. The amino acid sequence of the related protein for regulating and controlling plant anther and pollen development is shown as SEQ ID NO: 1. A wheat photo-thermo-sensitive male sterile line BS366 is used as an experimental material, the related gene TaABCG7-8 for regulating and controlling anther and pollen development is obtained and is subjected to gene silencing in wheat, and the result shows that anther cracking is abnormal and pollen development is abnormal. The related protein for regulating anther and pollen development has very important theoretical and practical significance on wheat reproductive development and promotion of a wheat heterosis utilization process.

The invention discloses a training group efficient construction method for waxy corn whole genome selective breeding. The method comprises the following steps: widely collecting common inbred lines or DH lines or inbred lines or DH lines subjected to genetic improvement in waxy corn breeding; carrying out phylogenetic tree, population structure and pedigree analysis on the collected waxy corn inbred line or DH line; dividing the inbred line or the DH line into a plurality of heterosis groups based on a phylogenetic tree, pedigree information and a group structure analysis result; respectively selecting a plurality of representative inbred lines from the heterosis groups; preparing a plurality of hybrids from a plurality of representative inbred lines or DH lines by adopting a sparse part dicolumn hybridization design method among different heterosis groups; performing multi-environment planting identification on the plurality of hybrids to obtain a plurality of character data; predicting phenotypes of all possible hybrids assembled by a plurality of representative inbred lines; the general combining ability effect of each representative inbred line or DH line is estimated through the predicted phenotype of the hybrid, and parent inbred line materials with high general combining ability are screened out. The method solves the problem that the predictability of newly introduced germplasm or germplasm with relatively far genetic relationship is reduced due to the fact that a training group used in the existing waxy corn whole genome selective breeding only contains a hybrid combination of a few backbone inbred lines and lacks representativeness.

The invention relates to the technical field of breeding, in particular to a method for breeding a sterile line of japonica rice with wide affinity, high exposure and early blossoming. According to the method, through combination of multi-parent hybridization and multiple cross with marker-assisted selection and phenotype tracking, accurate polymerization of multiple characters such as wide affinity, high exposure and early blossoming is realized, the breeding efficiency is high, the bred 'Ning46A' is high in seed production suitability, can be matched with an indica-japonica restorer line, fully utilizes the heterosis between subspecies, and has important production and application values.

The invention belongs to the technical field of livestock breeding, and particularly relates to a rapid breeding method for a new variety of medium-sized golden Luhua commercial chickens. The breeding method comprises the following steps: hybridizing a first female parent and a first male parent to obtain an F1-generation group; selecting F1-generation female individuals as backcross female parents, and carrying out grading hybridization on the backcross female parents and the first male parents or chickens of the same species to obtain F2-generation groups; and selecting the F2-generation male individual as a terminal male parent, and carrying out three-way hybridization on the terminal male parent and the second female parent to obtain commercial-generation chickens. According to the breeding method, through the combination of grading hybridization and three-way hybridization, the excellent quality characters are consolidated firstly, then the three-way hybridization is used for stimulating the heterosis, the bred commercial chickens have the advantages of being high in growth speed, moderate in body type, delicious in meat quality and the like, and the breeding method is clear in breeding path and high in commercial value. In addition, the breeding method effectively solves the technical problem that the existing chicken breeds are difficult to consider a plurality of excellent characters such as excellent meat quality and rapid growth.

The application relates to the field of plant breeding, and specifically provides a method for fixing hybrid vigor of rice by using an OsMPF gene. An expression cassette of an OsECA1 promoter driven OsMPF gene is first constructed; an expression cassette of three target CRISPR / Cas9 knockout of rice OsPAIR1, OsREC8 and OsOSD1 is then constructed; the above expression cassettes are integrated into the same vector to transform hybrid rice; three-gene homozygous mutant and OsMPF positive plants are screened, and diploid clone offspring are identified through flow cytometry and genome sequencing. The method provides a new method for fixing hybrid vigor of apomixis of rice by combining the rice OsMPF gene with MiMe, the method can successfully fix the hybrid vigor of rice, can obtain an apomixis system with high seed setting rate, and provides a new solution for fixing hybrid vigor of rice apomixis.

The invention provides a three-line auto-sexing mating method for meat and egg quails, belongs to the technical field of quail breeding, and provides two sets of three-line auto-sexing mating methods for meat and egg quails, which are characterized in that three-line hybridization of Chinese yellow-feather quails, Beijing white-feather quails and Difagke (FM-line) quails is utilized, and two consecutive generations are utilized to realize auto-sexing. After the young quail is hatched, the male quail and the female quail can be distinguished according to the feather color, the male quail and female quail distinguishing means are greatly simplified, the feeding management efficiency is improved, F1 and F2 represent obvious heterosis, the body type of the male quail is obviously increased, the meat production performance is obviously improved, the egg laying performance of the female quail is good, and the method is a high-quality new quail commercial line.

This invention discloses a recessive nuclear male sterility gene. BoaGMS1 Application in regulating male development in Chinese kale. This invention screens recessive nuclear male sterility mutants from Chinese kale breeding and isolates the Chinese kale male fertility gene using forward genetics. BoaGMS1 Further experiments confirmed that BoaGMS1 A gene mutation resulted in recessive nuclear male sterility in Chinese kale. Meanwhile, targeting... BoaGMS1 Cosegregating molecular markers developed for male-sterile lines can be used for identifying fertility alleles in plants, screening target plants in marker-assisted breeding, and determining seed purity. Furthermore, they can be used in the development of kale. BoaGMS1 Male-sterile lines can be applied to the breeding and production of new hybrid varieties of Chinese kale. Therefore, the male fertility gene for Chinese kale provided by this invention... BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS It plays an important role in the utilization of heterosis and hybrid seed production of Chinese kale and even Brassica oleracea vegetables.

The application discloses a tomato RNA methylation modification recognition protein SlECT2 and application thereof, and belongs to the field of plant genetic engineering and molecular breeding. The amino acid sequence of the SlECT2 protein is shown as SEQ ID No. 1. The SlECT2 gene is expressed in tomato plants, and the expression amount in reproductive organs is relatively high, and the expression amount in mature anthers is higher than that in young anthers. In the application, a tomato strain with specific knockout of the SlECT2 gene is obtained by a genetic engineering method, and the knockout mutant has abnormal pollen development at normal temperature, but the plant vegetative growth and female reproductive organs develop normally. The gene can be applied to utilization of tomato heterosis as a functional gene for regulating tomato pollen development, can reduce the hybrid seed production cost of excellent hybrid F1 generation, and has great development and utilization value.

The application discloses a binary pig efficient breeding method based on hybridization advantage and application, relates to the technical field of livestock breeding, and comprises the following steps: acquiring phenotype data of pure line Landrace pigs, pure line Large White pigs and historical hybrid offspring, setting the weight of reproduction traits, growth traits and feed efficiency traits in combination with external environment parameters, and generating comprehensive heterosis evaluation indexes; performing high-density genotyping on candidate Landrace pigs and candidate Large White pigs, constructing a genetic effect prediction model based on the genotyping results and the phenotype data, predicting the hybridization effect of a pairing combination of Landrace pigs and Large White pigs by using the genetic effect prediction model, screening the pairing combination meeting the comprehensive heterosis evaluation indexes according to the hybridization effect, and implementing artificial insemination to obtain first-generation binary pigs; and performing whole life cycle performance determination on the first-generation binary pigs, and calculating the measured comprehensive heterosis evaluation indexes.

The invention belongs to the technical field of agricultural biology, and particularly relates to an InDel molecular marker closely linked with a rice hybrid mortality gene HED2 as well as a primer and application of the InDel molecular marker. The InDel molecular marker comprises a molecular marker C1R2.6 and / or a molecular marker C1R2.9, the base sequence of the molecular marker C1R2.6 is as shown in SEQ ID NO: 1, and the base sequence of the molecular marker C1R2.9 is as shown in SEQ ID NO: 2. According to the invention, a gene HED2 which is located on a long arm of a first chromosome, comes from inter-indica rice subspecies and controls hybrid mortality is located for the first time, and a closely linked InDel marker molecule and a primer for amplifying the InDel molecular marker are obtained. Whether the variation of the hybrid mortality gene HED2 exists or not can be judged only by detecting the characteristics of an amplification band so as to predict the phenotype of the rice after hybridization, and the method can be used for guiding the breeding work of excavating and utilizing the heterosis of the rice.

The application discloses a method for breeding and multiplying a flue-cured tobacco cytoplasmic male sterile line MS Guiyan 19, which takes the flue-cured tobacco cytoplasmic male sterile line MSG28 as a cytoplasmic male sterile gene source, takes MSG28 as a female parent, takes a variety Guiyan 19 as a recurrent parent, and is bred into a new cytoplasmic male sterile line MS Guiyan 19 through continuous backcrossing for six generations after hybridization; and the cytoplasmic male sterile line MS Guiyan 19 is bred by taking the sterile line MS Guiyan 19 as the female parent and taking the maintainer line Guiyan 19 as the male parent and is bred and preserved through hybridization. The flue-cured tobacco sterile line MS Guiyan 19 bred by the application has the advantages of stable sterility, excellent agronomic characters, higher yield and output value, good easy-curing and curing properties, good appearance and quality of tobacco leaves, and coordinated chemical components, and provides a new excellent sterile line germplasm for flue-cured tobacco hybridization advantage utilization research work.

The invention discloses application of an SNP (Single Nucleotide Polymorphism) molecular marker located on a pig chromosome 2 and related to nipple number heterosis, the locus of the SNP molecular marker is rs334177868 and corresponds to Agt at 405366bp on a chromosome 2 of an international pig reference genome version 11.1; the genotype of the SNP molecular marker is AA, AG or GG. The SNP molecular marker provided by the invention can be applied to crossbreeding of pigs so as to improve the nipple number heterosis of offspring pigs and realize genetic improvement of the pigs. According to the present invention, by detecting the single nucleotide polymorphism and / or the genotype of the SNP molecular marker, the identification of the pig nipple number character heterosis can be achieved, and by breeding the pig with the genotype of the SNP molecular marker GG, the breeding process of the pig can be accelerated, and the reproductive capacity of the sow can be improved.

The invention belongs to the technical field of plant biotechnology and crop molecular breeding, and particularly relates to an application and a method of a replication gene pair for inducing development of maize spontaneous endosperm and embryos, and the application of the replication gene pair for inducing development of maize spontaneous endosperm and embryos to ZmFIE develops a new approach for apomixis of maize. It is proved that ZmFIE2 (and the synergistic effect of ZmFIE2 and ZmFIE1) is a key factor for maintaining fertilization dependence of corn seed development, corn can be induced to generate autonomously developed endosperm and embryoid structures under the condition that exogenous genes are not introduced through mutation of the gene, and a core element is provided for fixing heterosis. An efficient germplasm innovation material is provided, the zmfie1 and zmfie2 double mutant shows more severe phenotype than a single mutant, endosperm cell differentiation in mutant grains is blocked, intracellular replication is remarkably reduced, mitotic activity is enhanced, and the strong cell cycle change is a characteristic necessary for constructing an efficient haploid induction line.

The invention relates to the technical field of genome information analysis, in particular to a local pig optimal heterosis prediction method based on genome information. According to the method, pure parents with clear genetic backgrounds are accurately identified and screened out by utilizing high-density SNP marker information covering a whole genome, a reference population is constructed, large-scale hybridization propagation is simulated, structured virtual population data is formed, and multi-character phenotype data of filial generations are standardized and weighted, so that the genetic backgrounds of the filial generations are optimized, and the genetic backgrounds of the filial generations are optimized. The method comprises the following steps of: dividing a plurality of characteristic groups such as a production performance type and a reproductive performance type, and analyzing the correlation between phenotypic deviation and genotype in each group, so as to separate and quantify dominant effect caused by heterozygous genotype and recessive effect caused by homozygous genotype, construct explicit and recessive weight parameters, and determine the genetic relationship between the dominant effect and the recessive effect; finally, the non-additive effect parameters are superposed to a traditional additive breeding value model, direct quantitative prediction of heterosis is achieved, and therefore the accuracy and efficiency of hybrid combination matching are greatly improved.

The invention relates to the technical field of plant molecular biology, in particular to a DP1 protein for regulating and controlling female fertility of rice as well as a coding gene and application of the DP1 protein. According to the invention, after the expression of the DP1 gene is destroyed, the rice has genic male sterility, the female setting rate is as low as 0.5%, the female sterility character is stable and is not influenced by environmental conditions, and the male sterility is completely normal; after the DP1 gene is further introduced, the female fertility of the rice can be restored. Therefore, the DP1 protein and the coding gene thereof play an important role in the aspects of utilizing the heterosis of crops and removing male parent seeds in hybrid seed production. The invention provides a new element for research and development of a novel hybrid breeding and seed production technology of rice, and has a wide application prospect.

The invention relates to the field of plant breeding, and particularly provides a method for fixing rice heterosis by using an OsMPF gene. The method comprises the following steps: firstly, constructing an OsMPF gene expression box driven by an OsECA1 promoter; then, an expression box of rice with three target points of OsPAIR1, OsREC8 and OsOSD1 being knocked out of CRISPR / Cas9 is constructed; integrating the expression cassette into the same vector to transform hybrid rice; screening three-gene homozygous mutation and OsMPF positive plants, and identifying diploid cloned offspring through flow cytometry and genome sequencing. According to the method, the rice OsMPF gene is combined with MiMe to provide a novel method for fixing the heterosis of the rice through apomixis, the heterosis of the rice can be successfully fixed through the method, an apomixis system with the high maturing rate can be obtained, and a novel solution is provided for fixing the heterosis of the rice through apomixis.

The invention belongs to the technical field of pig molecular markers, and particularly discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to the character of the number of live piglets born by pigs and application of the SNP molecular marker. Gene typing is carried out by utilizing a gene chip technology, whole genome association analysis is carried out on the basis of SNP molecular markers, and four SNP molecular markers related to the character of the live litter size of the pig are screened out through the analysis. The SNP molecular marker is located in a pig chromosome 2, is annotated to a PPP2R2B gene segment, and can be used for marker-assisted breeding of pig live birth number traits and heterosis utilization thereof.

The invention discloses an InDel marker located on a chromosome 9 of a pig and capable of regulating and controlling the papillary number heterosis of the pig and application of the InDel marker. The nucleotide sequence of the InDel marker is shown as SEQ ID NO: 1 or SEQ ID NO: 2; the site is located between the 79th site and the 80th site from the 5'end of the nucleotide sequence shown in SEQ ID NO: 1, and corresponds to insertion mutation of C between the 11197897 bp and the 11197898 bp on the No.9 chromosome of the international pig reference genome version 11.1. The InDel marker provided by the invention can be applied to crossbreeding of pigs so as to improve the nipple number heterosis of offspring pigs and realize genetic improvement of the pigs, and by breeding the pigs with the InDel marker genotype of T / T, the reproductive capacity of sows can be remarkably improved, and the number of weaned piglets can be increased.

The application relates to a method for creating a fertile tetraploid hybrid between a cultivated rice and an Oryza australiensis genome, which comprises the following steps: using CX35-2X or YZ32-2X as a female parent material, and Oryza australiensis EE as a male parent material to perform hybridization, thereby obtaining first-generation hybrid seedlings; doubling the first-generation hybrid seedlings by colchicine, thereby obtaining hybrid tetraploid seeds. The application utilizes the whole genome transfer and polyploidization, that is, a distant hybrid containing a complete wild rice genome is obtained through distant hybridization, and then a hybrid allopolyploid is obtained through chromosome doubling, so that the excellent genes of the wild rice and the distant hybridization advantage of the polyploid are fully utilized.

The invention discloses application of an InDel molecular marker located on a pig chromosome 16 and related to nipple number heterosis, the locus of the InDel molecular marker is rs793470896, and the InDel molecular marker corresponds to GGAGGCACgt at the 74359589 bp position on the chromosome 16 of an international pig reference genome version 11.1; the gene type of the InDel molecular marker is GGAGGCAC / GGAGGCAC, GGAGGCAC / G or G / G. The gene type of the InDel molecular marker is GGAGGCAC / GGAGGCAC. The InDel molecular marker provided by the invention is used for marker-assisted selective breeding, pigs with the genotype of GGAGGCAC / GGAGGCAC in a group are gradually eliminated, and the nipple number of bred sows can be remarkably increased, so that the piglet breeding and lactation capabilities of the sows can be remarkably improved, the survival rate of piglets can be increased, and the production income can be increased.