12 results about "Heterosis" patented technology

The application discloses a kind of based on molecular marker clustering analysis's cotton hybrid breeding combination screening method, the screening method includes: step S1: using multi-traits related molecular marker to the gene typing experiment of test cotton variety, data collection and arrangement are carried out;Step S2: excellent allele frequency calculation and feature matrix construction;Step S3: data standardization and principal component analysis dimension reduction;Step S4: optimal cluster number selection and genetic clustering;Step S5: each population character analysis;Step S6: heterosis potential score and combination screening.The technical problem that present cotton breeding parent selection lacks multi-traits comprehensive quantitative evaluation is solved, and the data of breeding decision-making and intelligentization are realized.

The application relates to the field of plant breeding, and specifically provides a method for fixing hybrid vigor of rice by using an OsMPF gene. An expression cassette of an OsECA1 promoter driven OsMPF gene is first constructed; an expression cassette of three target CRISPR / Cas9 knockout of rice OsPAIR1, OsREC8 and OsOSD1 is then constructed; the above expression cassettes are integrated into the same vector to transform hybrid rice; three-gene homozygous mutant and OsMPF positive plants are screened, and diploid clone offspring are identified through flow cytometry and genome sequencing. The method provides a new method for fixing hybrid vigor of apomixis of rice by combining the rice OsMPF gene with MiMe, the method can successfully fix the hybrid vigor of rice, can obtain an apomixis system with high seed setting rate, and provides a new solution for fixing hybrid vigor of rice apomixis.

This invention discloses a recessive nuclear male sterility gene. BoaGMS1 Application in regulating male development in Chinese kale. This invention screens recessive nuclear male sterility mutants from Chinese kale breeding and isolates the Chinese kale male fertility gene using forward genetics. BoaGMS1 Further experiments confirmed that BoaGMS1 A gene mutation resulted in recessive nuclear male sterility in Chinese kale. Meanwhile, targeting... BoaGMS1 Cosegregating molecular markers developed for male-sterile lines can be used for identifying fertility alleles in plants, screening target plants in marker-assisted breeding, and determining seed purity. Furthermore, they can be used in the development of kale. BoaGMS1 Male-sterile lines can be applied to the breeding and production of new hybrid varieties of Chinese kale. Therefore, the male fertility gene for Chinese kale provided by this invention... BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS It plays an important role in the utilization of heterosis and hybrid seed production of Chinese kale and even Brassica oleracea vegetables.

The application discloses a method for breeding and multiplying a flue-cured tobacco cytoplasmic male sterile line MS Guiyan 19, which takes the flue-cured tobacco cytoplasmic male sterile line MSG28 as a cytoplasmic male sterile gene source, takes MSG28 as a female parent, takes a variety Guiyan 19 as a recurrent parent, and is bred into a new cytoplasmic male sterile line MS Guiyan 19 through continuous backcrossing for six generations after hybridization; and the cytoplasmic male sterile line MS Guiyan 19 is bred by taking the sterile line MS Guiyan 19 as the female parent and taking the maintainer line Guiyan 19 as the male parent and is bred and preserved through hybridization. The flue-cured tobacco sterile line MS Guiyan 19 bred by the application has the advantages of stable sterility, excellent agronomic characters, higher yield and output value, good easy-curing and curing properties, good appearance and quality of tobacco leaves, and coordinated chemical components, and provides a new excellent sterile line germplasm for flue-cured tobacco hybridization advantage utilization research work.

The application discloses application of an InDel molecular marker related to body height heterosis on chromosome 10 of a pig, a site of the InDel molecular marker is rs712433118, corresponding to an A>ATT mutation at 341376 bp on chromosome 10 of an international pig reference genome 11.1 version, and genotypes of the InDel molecular marker are A / A, A / ATT or ATT / ATT. By using the InDel molecular marker provided in the application for marker-assisted selection breeding, body height traits of the pig can be accurately selected and breeding process of a three-way crossbred pig is accelerated.

This invention discloses tomatoes SlMKK2 The application of genes in creating male-sterile tomato lines falls under the field of genetic engineering. This invention has found that, compared to wild-type control tomato plants, SlMKK2 The knockout plants showed no obvious abnormalities in vegetative growth or floral organ morphology, but approximately 30% of the pollen grains were aborted, and the in vitro germination rate was significantly reduced. Aborted pollen grains exhibited abnormal morphology such as collapse and wrinkling, and the abortion process began in the late uninucleate stage. This invention verifies... SlMKK2 This gene is involved in the developmental regulation of tomato pollen transition from the uninucleate to binucleate stage. Its deletion can lead to impaired male fertility in tomatoes and can be used to create male-sterile tomato materials. This not only provides a new genetic resource for the creation of recessive male-sterile tomato materials but also offers a new technical approach for utilizing heterosis in tomatoes.

This invention relates to a male gamete protection gene S19g-A7 and its application in improving rice hybrid fertility and hybrid rice breeding. This invention identifies a male gamete protection gene S19g-A7 at the S19 locus of interspecific hybrid sterility in rice, whose encoded amino acid sequence is shown in SEQ ID NO.2. Introducing the S19g-A7 gene into Asian rice for functional complementation can break the S19 locus-mediated interspecific hybrid sterility in rice, rapidly creating hybrid compatible lines that overcome interspecific hybrid sterility in Asian and African rice. This provides genetic resources and breeding methods for utilizing distant hybrid vigor in high-quality rice germplasm breeding, and has significant application value.

The application belongs to the technical field of genetic engineering and plant genetic breeding, and particularly relates to application of a new tetraploid rice fertility gene NTFR2. The application uses a gene knockout technology to determine the function of the fertility regulation gene NTFR2 in the new tetraploid rice, and finds that the NTFR2 affects the fertility of the new tetraploid rice. When the function of the NTFR2 gene is lost, the seed setting rate of the mutant is reduced, and the development of the female gametophyte sac exhibits abnormality. Meanwhile, the loss of the function of the NTFR2 gene leads to a significant decrease in the fertility of the new tetraploid rice, but has a smaller influence on the male gametes and other agronomic traits, which indicates that the NTFR2 is a new gene mainly affecting the fertility of the tetraploid rice, and has an important application prospect in the fields of creating a new female sterile line and further promoting the utilization of hybrid vigor of the tetraploid rice.

The present application belongs to the technical field of preparation of excellent commercial seed of economic marine shellfish, and particularly relates to a high-efficiency method for preparing interspecific three-way hybrid of Argopecten genus scallop. The method uses a selected and purified male sterile and female fertile hybrid Argopecten mactrolineatus as a female parent, and a restocked Argopecten purpuratus as a male parent, to produce a "Argopecten mactrolineatus Argopecten purpuratus" three-way hybrid scallop commercial seed with hybrid vigor and outstanding stress resistance. The method can realize large-scale, continuous and efficient hybrid seed production, and has the advantages of simple operation, labor saving, low dependence on personnel, and the like. The hybridization rate is increased from less than 30% in the prior art to more than 90%, and the efficiency of interspecific hybrid seed production and the purity of the seed of the protandrous Argopecten genus scallop are significantly improved.

The application discloses a method for evaluating heterosis based on offspring pedigree record and individual phenotype data, and belongs to the field of animal breeding. Step 1: selecting pure line A male, pure line A female, pure line B male and pure line B female for evaluating heterosis, and recording the individual numbers of pure line A and pure line B, wherein each individual number needs to be an independent number; step 2: carrying out pure line cross experiment and pure line closed breeding experiment, and the two experiments have no sequence; step 3: using the data collected in step 2, calculating the heterosis by a mathematical model when the cross offspring generation and the parent pure line are fed in different environments. The application solves the problem that the traditional heterosis calculation method is not applicable when the cross offspring generation and the parent pure line are not fed in the same environment. The method can calculate the heterosis by feeding the cross offspring generation and the parent pure line respectively and using the offspring pedigree record and individual phenotype data.

The application discloses a hybrid breeding method by means of virtual traits. In order to overcome the uncertainty and blindness of the prior art of directly selecting and matching hybrid combinations according to the parent performance, the application comprises the following steps: S1, establishing a basic database comprising a hybrid first generation and corresponding parent performance; constructing a basic trait set and establishing an operation method set; S2, randomly selecting a basic trait and an operation method from the basic trait set and the operation method set to combine and construct a selected virtual trait; S3, calculating the parameters and test results of the practical trait and the selected virtual trait by using the data in the basic database; S4, comparing the calculated parameters and test results with corresponding threshold values to select a target virtual trait for selection or matching of a hybrid combination. The application avoids the influence of hybrid advantage during the selection of parents and the matching of hybrid combinations, accelerates the selection process of parents, reduces the number of matched hybrid combinations, and accelerates the breeding process.

The application discloses a method for cultivating saline-alkali tolerant hybrid barley seeds based on barley three-line method, takes a barley male sterile line parent 270A as a sterile source, takes a known saline-alkali tolerant variety Yanshima No. 11 as a recurrent parent and a maintainer line, introduces a major saline-alkali QTL site carried by Yanshima No. 11 into the sterile line background through continuous multiple generations of backcrossing, simultaneously fixes the agronomic traits, and finally breeds a stable saline-alkali tolerant male sterile line Yanshima No. 16; then takes Yanshima No. 11 as the maintainer line, plants parent and offspring in a 2:1 ratio in a saline-alkali stress field, implements artificial assisted pollination and dynamic hybrid removal during the heading stage to the flowering stage, and harvests seeds formed by sterile line plants, so that saline-alkali tolerant hybrid barley F1 generation seeds are obtained. The application realizes precise coupling of the saline-alkali tolerance of barley and the three-line matching system for the first time, overcomes the bottleneck of long breeding period and difficult utilization of hybrid vigor in traditional saline-alkali tolerance breeding, and the F1 seeds produced have a yield increase of 23.6% under saline-alkali soil compared with conventional varieties.