The invention discloses health wine for females. The health wine is prepared from the following raw materials in parts by weight: 120 to 150 parts of grapes of Hutai No.8, 20 to 40 parts of blueberries, 10 to 20 parts of colla corii asini powder, 20 to 30 parts of red dates, 5 to 10 parts of rose flower petals, 5 to 10 parts of leonurus, 5 to 8 parts of lavender, 20 to 30 parts of brown sugar, 10 to 15 parts of lycium barbarum, 5 to 10 parts of codonopsis pilosula, 5 to 10 parts of angelica sinensis, 1 to 3 parts of peanuts and 1 to 3 parts of walnuts. Furthermore, the invention further provides a preparation method of the health wine for females. The health wine f has the effects of benefiting qi, nourishing blood, dispersing liver, promoting blood circulation, promoting blood circulation to remove blood stasis and regulating menstruation and qi, has a function of supplementing plant protein and is particularly suitable for health care of adult females; if a climacteric woman drinks the wine for a long time, the effect of delaying the climacterium is achieved, and discomforts caused by the climacterium can be reduced; besides, a process for preparing the health wine for females is simple; raw materials are all daily medicines; and therefore, the health wine is low in cost and good in effect and does not have side effects.