3 results about "Cross hybridization" patented technology

The invention relates to a preparation method of a space transcriptome chip and the space transcriptome chip, and the preparation method comprises the following steps: synthesizing a target analysis unit by adopting a ligase-based connection synthesis method or an amplification enzyme-based amplification synthesis method, the probe sequence on the microsphere comprises a cleavable primer, an amplification primer, a bar code fragment region, a molecular tag and an mRNA capture region; the target analysis unit is randomly laid on a micropore array chip, the micropore array chip comprises a preset number of micropore arrays, and each micropore array comprises a plurality of micropores arranged according to a preset arrangement mode; and carrying out a cross-hybridization experiment on the microspheres on the micropore array chip by using the fluorescent sequences complementary with the bar code fragments on the microspheres to obtain sequence information and distribution information of each microsphere. The probe on a single analysis unit is modified in a large-scale chemical synthesis mode, operation is easy, the requirement for instruments is low, the treatment flux is high, and the preparation speed is high.