5 results about "Cross hybridization" patented technology

The present invention relates to a method for the multiplexed detection of nucleic acids in a sample. This is accomplished by using novel sets of primer pairs containing tails which can be selectively captured by complementary tail sequences with negligible cross-hybridization. The invention relates also to reagents, kits and devices used to implement the multiplexed detection method.

The invention relates to a preparation method of a space transcriptome chip and the space transcriptome chip, and the preparation method comprises the following steps: synthesizing a target analysis unit by adopting a ligase-based connection synthesis method or an amplification enzyme-based amplification synthesis method, the probe sequence on the microsphere comprises a cleavable primer, an amplification primer, a bar code fragment region, a molecular tag and an mRNA capture region; the target analysis unit is randomly laid on a micropore array chip, the micropore array chip comprises a preset number of micropore arrays, and each micropore array comprises a plurality of micropores arranged according to a preset arrangement mode; and carrying out a cross-hybridization experiment on the microspheres on the micropore array chip by using the fluorescent sequences complementary with the bar code fragments on the microspheres to obtain sequence information and distribution information of each microsphere. The probe on a single analysis unit is modified in a large-scale chemical synthesis mode, operation is easy, the requirement for instruments is low, the treatment flux is high, and the preparation speed is high.

The present invention discloses an electrochemical sensor for detecting EGFR gene mutation and a preparation method thereof, and belongs to the field of biosensor technology. The present invention solves the problems of complex data processing, high cost, long detection cycle and high invasiveness in the detection method of EGFR mutant genes in the prior art. The present invention designs a pair of stem-loop sequence primers to replace conventional LAMP primers, avoiding nonspecific amplification caused by cross-hybridization between multiple primers; GDY-Au TNPs are used as a sensing platform to improve detection sensitivity, and methylene blue-single-stranded DNA is modified as a reporter gene. When the target DNA is present in the LAMP product, a DNA / crRNA / Cas12a triplet complex is formed, the trans-cutting ability of Cas12a is activated, the reporter gene is cut, the redox mediator on the electrode is changed, and the entire signal change is detected by an electrochemical method. The present invention achieves the purpose of detecting EGFR gene mutations instantly, quickly and at low cost, and provides strong technical support for clinicians to diagnose diseases and evaluate treatment effects.