2 results about "Hpv genotyping" patented technology

The invention relates to a method, uses, a system, and a kit for detecting a human papillomavirus (HPV) genotype associated with the development of cervical, oral, or anogenital cancer in a subject and for determining the HPV genotype or genotypes thus detected in the same. Additionally, the present invention relates to uses of direct and / or reverse amplicons or primers generated by multiplex real-time PCR comprising a molecular tag for identifying a marker of human papillomavirus genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and / or 68.

This invention discloses a rapid on-site HPV genotyping method based on one-pot RPA-Cas12a, belonging to the field of molecular biology diagnostic technology. This invention utilizes the automated target screening tool AutoCORDS to design specific crRNAs and RPA primers for different HPV subtypes. Combined with RPA amplification and CRISPR-Cas12a, it achieves highly sensitive, highly specific, efficient, and convenient HPV genotyping detection. This method requires no complex instruments and has advantages such as simple operation, fast analysis speed, and independence from expensive equipment. It can accurately detect genotypes of 14 high-risk HPV types and is suitable for screening and diagnosis of HPV infection in resource-scarce areas.