14 results about "Hpv types" patented technology

Methods for treating human papillomavirus (HPV) type 16- or HPV type 18-associated high-grade squamous intraepithelial lesions (HSIL) of the cervix are described

This invention relates to a homogeneous photocatalytic chemiluminescence detection system for detecting HPV vaccine antigen content, its preparation method, and its application. The system utilizes self-made carboxyl-modified donor microspheres and receptor microspheres, coupled with HPV type-specific monoclonal antibodies via EDC / NHS activation, achieving one-step homogeneous detection. By optimizing the microsphere size, a 200 nm microsphere system was found to exhibit higher luminescence signal, a wider detection range, and better sensitivity when detecting HPV16 / 18 VLPs. This method is simple to operate, requires no plate washing, and possesses good specificity, accuracy, and precision. It is suitable for the in vitro relative potency and identification detection of bivalent HPV vaccines, and can replace traditional ELISA methods, reducing detection costs and safety risks. It is also suitable for large-scale applications in vaccine quality control.

This invention belongs to the field of biological detection technology, and particularly relates to a reproductive tract HPV-mRNA-E6 / E7 detection system and method based on nanopore sequencing. The system includes a sample pretreatment module, an RNA extraction and purification module, a reverse transcription module, a nanopore sequencing module, and a bioinformatics analysis module. The method includes the following steps: (1) reproductive tract sample collection and pretreatment; (2) extraction and purification of HPV-mRNA in the sample; (3) reverse transcription of the purified HPV-mRNA into cDNA; (4) nanopore sequencing of the cDNA to obtain raw sequencing data; (5) filtering, comparing, and annotating the sequencing data through the bioinformatics analysis module to achieve HPV type identification and quantitative analysis of E6 / E7 gene expression levels. This invention utilizes the advantages of nanopore sequencing, such as long read length and real-time detection, combined with the specificity of E6 / E7 mRNA as a biomarker of active infection. It can directly detect latent and active HPV infection, improve detection sensitivity, accurately distinguish infection types, and shorten the detection cycle. It enables rapid, accurate, and high-throughput detection of active HPV infection in the genital tract, providing reliable technical support for early screening, disease monitoring, and prognostic assessment of HPV-related diseases such as cervical cancer.

The present disclosure provides, inter alia, a vaccine composition comprising a squalene nanoemulsion (SNE) adjuvant and at least one type of virus-like particle (VLP) of human papilloma virus (HPV) selected from the following HPV types: 6, 11, 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 55, 56, 58, 59, 66, 68, 73, and 82.

The application discloses a quality control product for human papillomavirus molecular detection and a preparation method thereof, and belongs to the technical field of biological products. After synthesizing 25 types of HPV full genomes of high-risk types (HPV16, HPV18, HPV26, HPV31, HPV33, HPV35, HPV39, HPV45, HPV51, HPV52, HPV53, HPV56, HPV58, HPV59, HPV66, HPV68, HPV73, HPV82) and low-risk types (HPV6, HPV11, HPV42, HPV43, HPV44, HPV81, HPV83), the HPV full genomes are connected into a pcDNA3.1(+) skeleton vector system, the HPV full genomes are integrated into the genome of a human ovarian cancer cell line ovcar3 respectively, and 25 strains of human ovarian cancer cell lines stably integrating the HPV full genomes are obtained. The new quality control product or kit of various types of simulated clinical samples such as genomic DNA, cell precipitate and cell suspension can be prepared and is suitable for various detection platforms. The human cell line quality control product stably integrating the full-length genomes of the 25 HPV types can more accurately determine the viral load and control the whole process of HPV detection.

The present method allows for detection of at least nine HPV types in a real-time manner in one reaction vessel by analyzing signals measured at three temperatures using at least three different types of fluorescent labels. In particular, the method of the present invention can detect at least nine target nucleic acids in a real-time manner without performing a melting analysis.

The application discloses application of primers and a combination of probes in preparation of a kit for detecting HPV, wherein the nucleotide sequence of the primers is shown as SEQ ID NO: 15-42; and the nucleotide sequence of the probes is shown as SEQ ID NO: 1-14. The application overcomes problems of long detection time, high false negative rate and high operation technical threshold in traditional cervical cancer screening methods, provides primers and probes for detecting 14 high-risk HPV types, and provides a corresponding detection kit, which not only simplifies the operation process, reduces the cost and technical complexity, but also greatly shortens the time of cervical cancer screening, has high specificity, is suitable for large-scale preliminary screening of cervical cancer in clinical application, and has popularization and application value.

The present disclosure provides, among other things, a pharmaceutical composition that includes a lipid nanoparticle adjuvant and an anti-human papillomavirus (HPV) comprising HPV virus-like particles (VLPs) of at least one type of human papillomavirus (HPV) selected from the group consisting of HPV types: 6, 11, 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 55, 56, 58, 59, 66, 68, 73, and 82.

This invention relates to the field of biological detection, and more particularly to primer combinations, reagent kits, and their applications. The invention provides primer combinations including: primer set I, primer set II, primer set III, primer set IV, primer set V, primer set VI, primer set VII, primer set VIII, primer set IX, primer set X, primer set XI, primer set XII, primer set XIII, primer set XIV, primer set XV, primer set XVI, primer set XVII, primer set XVIII, primer set XIX, primer set XX, primer set XXI, primer set XXII, and primer set XXIII. This invention also provides a reagent kit for rapid HPV detection. This kit can rapidly detect the above 23 HPV types within 20 minutes. The detection limit of the kit is up to 10. 3 The accuracy and specificity are good, with a per-copy / mL ratio.

The invention belongs to the technical field of HPV (human papillomavirus) detection, and particularly relates to a primer probe group and a kit for specific recognition and rapid detection of fourteen high-risk HPVs based on an RPA (recombinase polymerase amplification) isothermal amplification method, and application of the primer probe group and the kit. The primer probe group comprises a primer group and a probe group, wherein the primer group comprises a peripheral upstream primer with a nucleotide sequence as shown in SEQ ID NO: 1, a peripheral downstream primer with a nucleotide sequence as shown in SEQ ID NO: 2 and a peripheral downstream primer with a nucleotide sequence as shown in SEQ ID NO: 3; the nucleotide sequence of the peripheral downstream primer is as shown in SEQ ID NO: 2. The primer probe set and the kit have high sensitivity and high specificity, the detection sensitivity of the primer probe set and the kit to HPV high-risk viruses can reach 100 copies / mL, and the performance is superior to that of existing detection products in the market; the design of the specific probe can accurately detect fourteen high-risk HPV types, and does not generate cross reaction with low-risk HPV types or other viruses. The whole detection process is carried out at room temperature, laboratory operation is not needed, the detection time is about 30 minutes, and the detection can be completed in one detection rod.

The invention provides a fusion gene and an HPV nucleic acid vaccine. A fusion gene is obtained through research and optimization, and the fusion gene is obtained by sequentially connecting an L1 gene of an HPV 16 type, an E5 gene, an E6 gene and an E7 gene of the HPV 16 type and an E5 gene, an E6 gene and an E7 gene of an HPV 52 type. The inventor finds that an HPV vaccine prepared by taking the fusion gene as an antigen component can effectively promote cellular immune response and formation of long-term protective memory, promote hair growth center response and formation of humoral immune memory, remarkably improve the proportion of NKT cells, successfully activate systemic anti-tumor cell immune response of mice, and improve the anti-tumor effect of the mice. The anti-tumor immune effect is greatly improved. The traditional Chinese medicine composition has the dual effects of prevention and treatment, has the prevention capability, can effectively inhibit tumor growth and has the tumor treatment capability. The HPV vaccine also has the advantages of high safety, good stability and the like, and has a wide application prospect.

The invention provides a multiple RPA-CRISPR detection system aiming at human papilloma virus typing, the multiple RPA-CRISPR detection system comprises an RPA isothermal amplification module, a CRISPR multiple detection module and a signal output module, the RPA isothermal amplification module comprises RPA amplification primers targeting eight HPV subtypes 16, 18, 11, 31, 33, 45, 52 and 58 and two freeze-drying balls, and one-tube multiple amplification is carried out on a sample; the CRISPR multiple detection module comprises a multiple microcavity device, a detection system pre-embedded in the multiple microcavity device and a constant-temperature heating pad; and the signal output module is a lateral flow chromatography test strip. The invention also provides an application of the multiple RPA-CRISPR detection system and a detection method of the multiple RPA-CRISPR detection system. According to the invention, a plurality of reaction steps and reagents are integrated by virtue of the micro-fluidic chip, so that POC detection of the eight high-risk HPVs is realized.

The application provides a primer composition for detecting different types of human papilloma virus and application thereof, and relates to the technical field of nucleic acid determination or detection methods.The primer composition provided by the application comprises a first primer and a second primer for detecting HPV type 16, a third primer and a fourth primer for detecting HPV type 18, and a fifth primer and a sixth primer for detecting HPV high-risk types.The primer composition provided by the application can realize multiplex detection of human papilloma virus, has the characteristics of good specificity, high sensitivity and good repeatability, and can avoid the false positive problem existing in conventional primers.Based on the primer composition, the application further combines an immunochromatography test strip detection technology to realize rapid and efficient detection of human papilloma virus, and the operation is simple and relatively portable, and the application can be used for home self-detection.

The invention belongs to the technical field of biological medicine, and particularly discloses a human papilloma virus (HPV) 16 type E7 / E6E7 mRNA therapeutic vaccine and application thereof. HPV 16 type E7 mutant protein or E6E7 fusion protein and specific 5 'UTR, signal peptide, 3' UTR and poly (A) tail are inserted into modified pVAX-1 plasmid through a seamless cloning method to form an mRNA expression vector for expressing HPV E7 protein and / or E6E7 fusion protein, mRNA is synthesized after in-vitro transcription, and the mRNA vaccine is formed after liposome (LNP) encapsulation. The vaccine is administered through intramuscular injection or intratumor injection; or vaccine and chemical medicine are combined for administration. The vaccine provided by the invention can inhibit the growth of tumors, prolong the survival time of tumor-bearing mice, enhance cellular immune response and improve the tumor microenvironment, and shows huge potential for treating HPV-related cancers.