This invention relates to a homogeneous photocatalytic
chemiluminescence detection
system for detecting HPV
vaccine antigen content, its preparation method, and its application. The
system utilizes self-made carboxyl-modified donor microspheres and
receptor microspheres, coupled with HPV type-specific
monoclonal antibodies via EDC / NHS activation, achieving one-step homogeneous detection. By optimizing the
microsphere size, a 200 nm
microsphere system was found to exhibit higher
luminescence signal, a wider detection range, and better sensitivity when detecting HPV16 / 18 VLPs. This method is simple to operate, requires no plate washing, and possesses good specificity, accuracy, and precision. It is suitable for the
in vitro relative
potency and identification detection of bivalent
HPV vaccines, and can replace traditional ELISA methods, reducing detection costs and safety risks. It is also suitable for large-scale applications in vaccine
quality control.