Preparation method and application of HPV (human papillomavirus) rapid detection reagent

By combining magnetic nanoparticles and platinum nanoparticle catalytic materials with the CRISPR-Cas13a system, the problems of low pre-treatment efficiency and cumbersome detection steps caused by the complex sample matrix in HPV virus detection are solved, and efficient and portable viral RNA rapid detection is achieved, which is suitable for POCT applications.

CN120776060APending Publication Date: 2025-10-14JINGZHOU FIRST PEOPLES HOSPITAL
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Patent Information

Application Number
CN202510966959.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-07-14
Publication Date
2025-10-14

AI Technical Summary

Technical Problem

Existing HPV virus detection technology has low pre-treatment efficiency when faced with complex sample matrices, and the differences in amplification and detection incubation temperatures lead to cumbersome steps. In addition, the functions and flow dynamics of microfluidic chips are limited, making it difficult to achieve portable and efficient detection.

Method used

Magnetic nanoparticles combined with platinum nanoparticle catalytic materials are used to quickly separate HPV viral RNA through magnetic separation technology. The catalytic activity of platinum nanoparticles is used to drive the flow of fluid in the microfluidic chip. Combined with the CRISPR-Cas13a system, signal amplification and detection are achieved, and rapid detection is performed using a microfluidic disc chip.

Benefits of technology

It achieves efficient enrichment of low-copy viruses in complex samples, improves detection sensitivity and accuracy, simplifies operating procedures, reduces costs, and improves portability, making it suitable for POCT testing.

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Abstract

The invention provides a preparation method and application of a rapid HPV (human papillomavirus) detection reagent. The method comprises the following steps: 1, preparing a solution A; 2, magnetic stirring; 3, preparing platinum nanoparticles; 4, preparing a solution C; 5, preparing core-shell nanoparticles; and 6, preparing a rapid detection reagent, wherein the rapid detection reagent is used as an RNA trigger signal amplification reaction element in a fluorescent probe system and is applied to HPV vaccine curative effect evaluation. According to the method, RNA extraction and amplification in a complex sample are achieved through a Pt-coated Fe3O4-MOFs-RNA rapid detection reagent, efficient enrichment of low-copy viruses is achieved, meanwhile, a detection fluorescence signal is generated through efficient trans-cleavage activity of Cas13a, the performance of an HPV virus POCT detection technology is improved, the problem that a micro-fluidic chip is lack of liquid flowing power is solved, and the detection accuracy of the HPV virus POCT detection technology is improved. And an innovative idea is provided for application of CRISPR-based analysis in clinical diagnosis.
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