The invention discloses a nano-composite material used for tissue repair, which comprises segmented copolymer with electrical activity, nano-hydroxyapatite and normal tissue repair materials; wherein the segmented copolymer comprises aliphatic polyester and ailine pentamer, and the normal tissue repair materials comprises poly (lactide-co-glycolide), polylactide or polycaprolactone. The invention also discloses a preparation method of the nano-composite materials used for tissue repair, which comprises the following steps of: (a) dissolving the normal tissue repair material in a solvent, then adding the nano-hydroxyapatite into the solvent, and uniformly stirring to obtain a substrate material; and (b) dissolving the segmented copolymer into the solvent, then adding to the substrate material and mixing uniformly, and then precipitating and removing the solvent. The nano-composite material used for the tissue repair has the advantages of nontoxicity, biodegradability, processibility, sufficient mechanical strength, favorable electrical activity, and biocompatibility.