19 results about "Cell behaviour" patented technology

Provided herein are systems, devices, and methods for processing, analyzing, and classifying biological data sets and generation of cell profiles. The data sets may include multi-omic data. Some embodiments may include the use of machine learning in training a classifier of raw multi-omic data and incorporating system biology knowledge to understand cellular behavior and cell status at the biomolecular level.

The application discloses an animal cell adaptive tracking method and system based on deep learning, and belongs to the technical field of cell image analysis; a YOLOv10 deep learning model is combined with a Btrack adaptive tracking algorithm, animal cell samples are first dyed and microimage collection is carried out, a cell detection model is trained after image preprocessing, data labeling and augmentation, the model is converted into an ONNX format to realize efficient inference; then, Kalman filtering prediction and three-round Hungarian algorithm matching are carried out to complete cell adaptive tracking, the problems of cell occlusion, division, overlap and loss of tracking can be effectively handled, and stable and continuous cell motion trajectories are generated; the application can be deployed without GPU, has strong generalization ability, high detection and tracking precision, is suitable for scenes such as microecological research, clinical sample testing, drug effect evaluation and cell behavior analysis, and can significantly improve the automation level and stability of animal cell detection, counting and dynamic tracking.

The application relates to a preparation method and application of a Janus structure photothermal-piezoelectric hydrogel dressing for infected wound repair, and the preparation method comprises the following steps: phenylboronic acid grafted methyl acrylamide gelatin and dopamine grafted hyaluronic acid are respectively dissolved in deionized water, MXene@CeO2 heterojunction is uniformly ultrasonically dispersed, after mixing, sodium hydroxide is used to adjust the pH to be slightly alkaline, and a Janus structure inner layer is formed; phenylboronic acid grafted gelatin and ortho-nitrobenzyl alcohol grafted hyaluronic acid are respectively dissolved in deionized water, BaTiO3 is uniformly ultrasonically dispersed, and a Janus structure outer layer is formed after mixing. The Janus structure photothermal-piezoelectric hydrogel dressing prepared by the application has the characteristics of space-time release to adapt to the wound repair process, in addition, has excellent photothermal conversion performance, kills bacteria on the infected wound through photothermal heating, effectively simulates physiological electrical signals through piezoelectric effect, regulates cell behavior, and can be used for infected wound repair.

The invention relates to medicine, biology, veterinary, pharmacology diagnostics, agriculture, ecology, meterology, seismology, construction biotechnology, biomanufacturing and provided herein are products and methods for managing cells behavior, memory of cells and erasure of cell memory. The present invention describes products and methods that, unlike the known ones, make it possible by treating them with an RNase, a DNase, an antibody that binds to an RNA and / or DNA and / or a molecule with nuclease activity to control the properties of cells and organisms without the use of mutagens and / or the special introduction of genes and / or use of specific gene tools and / or changing its environmental conditions.

The invention relates to medicine, biology, veterinary, pharmacology diagnostics, agriculture, ecology, meteorology, seismology, construction biotechnology, biomanufacturing and provided herein are products and methods for managing cells behavior, memory of cells and erasure of cell memory. The present invention describes products and methods that, unlike the known ones, make it possible to control the properties of cells and organisms without the use of mutagens and / or the special introduction of genes and / or use of specific gene tools and / or changing its environmental conditions.

The invention relates to medicine, biology, veterinary, pharmacology diagnostics, agriculture, ecology, meteorology, seismology, construction biotechnology, biomanufacturing and provided herein are products and methods for managing cells behavior, memory of cells and erasure of cell memory. The present invention describes products and methods that, unlike the known ones, make it possible to control the properties of cells and organisms without the use of mutagens and / or the special introduction of genes and / or use of specific gene tools and / or changing its environmental conditions.

The application discloses a bone implant with a lampshade-expanding locking and self-energy-supplying coating and a preparation method thereof, and belongs to the medical implant field. The bone implant is designed according to the contraction structure of a lampshade, is in an elongated form below a martensite phase transition temperature, and is in an open lampshade form above an austenite phase transition temperature. The bone implant realizes self-adaptive expansion and surface locking in a marrow cavity by using the super-elasticity and shape memory effect of a memory alloy, and provides instant three-dimensional mechanical stability beyond traditional bone plates and intramedullary nails. The surface of the bone implant is coated with a functional gradient coating, which can actively capture biochemical energy and mechanical energy in the body, and intelligently and time-sequentially regulate growth factor release and cell behavior according to the biochemical energy and the mechanical energy, so that the continuous glucose metabolism and physiological activities in the body are directly converted into electric energy and control signals for driving bone healing, and energy self-provision and logic autonomy are realized.

The invention discloses a patterned cell culture substrate for cell behavior regulation and a preparation and use method thereof. The preparation method comprises the following steps: preparing the patterned cell culture substrate on the inner surface of a conventional cell culture substrate through a patterning technology; the patterned cell culture substrate has a three-dimensional microgroove structure. The three-dimensional microgroove structure can provide various three-dimensional microenvironments for cell growth, so that the contact guide effect of cells is triggered, the cell behaviors are regulated and controlled, and a good cell behavior regulation and control effect is achieved. By adopting the laser patterning technology, the photoetching technology and the electron beam photoetching technology, the rapid manufacturing of the patterned cell culture base material and the manufacturing of the cell culture base material with high-precision and high-complexity micro-nano scale patterns can be realized. Meanwhile, the problem that the pattern stability is insufficient when the patterned cell culture substrate is prepared by adopting a chemical modification method is avoided.

The present application relates to the field of medical materials, in particular to an artificial ligament and a preparation method thereof.The artificial ligament provided by the present application comprises a gel layer-artificial ligament composite system by preparing a layer of PVA hydrogel on the surface of the woven artificial ligament in situ, which has biological activity and anti-creep performance, and is beneficial to improve the medium and long-term clinical prognosis of the woven artificial ligament.The artificial ligament provided by the present application can also adjust the rigidity, thickness and micro-topology of the gel layer under cyclic loading, thereby producing different biological activity regulation effects, and there is a balance point of rigidity and micro-topology, so that the cell ingrowth induction effect is best.The artificial ligament provided by the present application has simple composition, only relies on biophysical clues to regulate cell behavior, avoids the biological safety problems caused by complex biological chemical components, and can simultaneously realize the biological activity improvement and anti-creep performance improvement of the traditional PET woven artificial ligament.

The invention provides gradient bionic hydrogel for osteochondral regeneration as well as a preparation method and application of the gradient bionic hydrogel. According to the invention, through organic-inorganic co-assembly of functionalized HA and CPO, the gradient bionic hydrogel (BiGOH) with a space nano structure and a component gradient is constructed. According to the gel, biomimetic mineralization of CPO under the concentration gradient can be achieved, hydroxyapatite (HAP) nanocrystals are distributed in space, and the length of the hydroxyapatite nanocrystals is gradually increased to 117.6 + / -24.73 nanometers from 23.09 + / -7.35 nanometers. The bionic gradient structure of BiGOH can spatially program recruitment of stem cells and macrophages so as to establish a regeneration-promoting microenvironment. The cell behavior can be spatially regulated and controlled through the component constructed by the bionic non-classical crystallized cluster precursor and the nanostructure gradient, so that a regeneration strategy with a very prospect is provided for repairing osteochondral defects.

The invention belongs to the cross technical field of biotechnology and biomedical engineering, and relates to a method and a device for detecting protein interaction in living cells, in particular to a method and a device for in-situ detection of protein-protein interaction (PPIs) in living cells at a single cell level and real-time correlation analysis of the PPIs and cell behaviors (such as migration and proliferation). The invention firstly provides a micro-fluidic chip system for interaction detection and real-time behavior monitoring of proteins in living cells. The micro-fluidic chip system comprises a micro-fluidic chip and a detection probe group reagent, the micro-fluidic chip comprises a single cell capture layer (100), a nano-film (200), a loading chamber layer (300) and an electrode layer (400). According to the invention, on the same chip platform, integration of single cell capture, molecule delivery, PPI detection, behavior monitoring, mechanism analysis and whole process is realized.

A 3D perfusion bioreactor system and method for more accurate modeling of in vivo environmental conditions and cellular behaviors. In some embodiments, the bioreactor includes a cavity for containing culture media and cells. A plurality of capillary tubes cross the cavity. Artery capillary tubes may transport culture media received from an inlet of the bioreactor, across the cavity, and into a return compartment. Vein capillary tubes may transport culture media from the return compartment, across the cavity, and to an outlet of the bioreactor. Metabolites may diffuse from the capillary tubes into the cavity for cellular consumption, and metabolic waste may diffuse from the cavity into the capillary tubes for removal from the bioreactor. In some embodiments, culture media discharged from the outlet may be processed via a waste treatment device and returned to the inlet.

The invention belongs to the field of material science and medicine, and relates to a preparation method and application of three-dimensional cell culture hydrogel capable of performing molecular-level accurate force stimulation on cells. The preparation method comprises the following steps: 1) respectively dissolving a polypeptide cross-linking agent and force application molecules in a first buffer solution to obtain a polypeptide cross-linking agent mixed solution and a force application molecule mixed solution; 2) dissolving the methacrylated glucan in a second buffer solution to obtain a methacrylated glucan mixed solution; and 3) fully reacting the force application molecule mixed solution with the methacrylated glucan mixed solution, and mixing with the polypeptide cross-linking agent mixed solution to obtain the three-dimensional cell culture hydrogel capable of performing molecular-level accurate force stimulation on cells. The hydrogel is good in biocompatibility and free of obvious cytotoxicity, and force application molecules can stimulate cells for a long time; the photoreaction is quick, the spatial-temporal resolution is realized, the cell behavior can be regulated and controlled in situ, the cell spreading and migration are promoted, and a real in-vivo mechanical environment is provided.

The application provides an engineered DNAzyme molecular machine and its application, including one or two groups of chain group one and chain group two, chain group one: i-motif chain modified DNAzyme and its substrate chain, the chain group one is modified on the surface of cancer cells and T cells, the acid microenvironment of tumor cells is used to make the modified i-motif chain contract, and the distance between the cancer cells and the tumor cells is shortened; chain group two: mitochondria-targeting peptide modified DNAzyme and its substrate chain, intracellular mitochondrial aggregation is used to induce apoptosis. The tumor microenvironment responsive control of cell behavior outside and inside cells based on DNAzyme includes cell recognition, intercellular proximity, separation after treatment and mitochondrial aggregation, which not only provides a useful way for regulating intercellular interaction, but also provides a powerful targeted cancer treatment method without killing normal cells.

The application belongs to the technical field of biomedicine, and particularly relates to a hydrogel for evaluating functions of corneal endothelial seed cells, and a preparation method and application thereof. The hydrogel is a three-dimensional cross-linked network structure formed by free radical polymerization of acrylamide in the presence of N,N'-methylene bisacrylamide. Meanwhile, extracellular matrix components are fixed on the surface of the three-dimensional cross-linked network structure, and the hydrogel is obtained. The hydrogel prepared by the application improves the problems of mismatching of mechanical properties of traditional materials, large batch difference and the like, and provides a more reliable standardized tool for HCE cell behavior research. This breakthrough technology solves the problem that traditional materials cannot simultaneously provide physical support and function evaluation.

This invention relates to the field of gene regulation technology, and discloses a method for constructing and applying a light-controlled CRISPRi gene regulation system. The light-controlled CRISPRi gene regulation system includes a biological circuit and a light source device; the biological circuit includes a light control module and a CRISPRi module; the light source device is an electronic device that emits red or green light. This invention introduces red and green light to precisely regulate CRISPRi, making its on / off state spatiotemporally specific. This invention helps to deepen the understanding of light-controlled gene circuits and has important guiding significance for constructing large-scale synthetic biology systems that can target multiple genes. In practical applications, the light-controlled CRISPRi gene regulation system can be integrated into cells, allowing it to turn the expression of specific genes on or off in real time as needed, regulating cellular behavior or metabolic pathways, saving production costs, and improving production efficiency.

The invention discloses a bone implant imitating lantern unfolding locking and a self-energized coating and a preparation method of the bone implant, and belongs to the field of medical implants.The bone implant is designed by imitating a contraction structure of a lantern, is in a slender state at the temperature lower than the martensite phase transformation temperature and is in an open lantern shape at the temperature higher than the austenite phase transformation temperature, and the self-energized coating is in a self-energized state. The superelasticity and the shape memory effect of the memory alloy are utilized, self-adaptive expansion and planar locking are achieved in a medullary cavity, and the instant three-dimensional mechanical stability superior to that of a traditional bone fracture plate and an intramedullary nail is provided. The surface of the bone implant is coated with a functional gradient coating, so that in-vivo biochemical energy and mechanical energy can be actively captured, growth factor release and cell behaviors can be intelligently and sequentially regulated and controlled according to the in-vivo biochemical energy and mechanical energy, and in-vivo continuous glucose metabolism and physiological activities are directly converted into electric energy and control signals for driving bone healing; and energy self-sufficiency and logic independence are realized.

The invention relates to the field of medical materials, in particular to an artificial ligament and a preparation method thereof. According to the artificial ligament provided by the invention, a layer of PVA hydrogel is prepared on the surface of the woven artificial ligament in situ to form a gel layer-artificial ligament composite system, and the system has biological activity and creep resistance and is beneficial to improving medium and long term clinical prognosis of the woven artificial ligament. According to the artificial ligament provided by the invention, the rigidity, the thickness and the microscopic topological structure of the gel layer can be adjusted at the same time through cyclic loading, so that different biological activity regulation and control effects are generated, and a balance point of the rigidity and the microscopic topological structure exists, so that the cell ingrowth induction effect is the best. The artificial ligament provided by the invention is simple in component, cell behaviors are regulated and controlled only through biophysical clues, the problem of biosafety possibly caused by complex biochemical components is avoided, and the bioactivity and creep resistance of a traditional PET woven artificial ligament can be improved at the same time.

The invention relates to medicine, biology, veterinary, pharmacology diagnostics, agriculture, ecology, meteorology, seismology, construction biotechnology, biomanufacturing and provided herein are products and methods for managing cells behavior, memory of cells and erasure of cell memory. The present invention describes products and methods that, unlike the known ones, make it possible to control the properties of cells and organisms without the use of mutagens and / or the special introduction of genes and / or use of specific gene tools and / or changing its environmental conditions.