39 results about "Cell behaviour" patented technology

Provided herein are systems, devices, and methods for processing, analyzing, and classifying biological data sets and generation of cell profiles. The data sets may include multi-omic data. Some embodiments may include the use of machine learning in training a classifier of raw multi-omic data and incorporating system biology knowledge to understand cellular behavior and cell status at the biomolecular level.

The invention belongs to the technical field of biomedicine, and particularly relates to hydrogel for evaluating corneal endothelium seed cell functions and a preparation method and application thereof.The hydrogel is of a three-dimensional cross-linked network structure formed by acrylamide in the presence of N, N '-methylene bisacrylamide through free radical polymerization; meanwhile, an extracellular matrix component is fixed on the surface of the three-dimensional cross-linked network structure, and the hydrogel is obtained. According to the hydrogel prepared by the method, the problems of mismatching of mechanical properties, large batch difference and the like of a traditional material are solved, and a more reliable standardized tool is provided for HCE cell behavior research. The breakthrough technology solves the problem that a traditional material cannot give consideration to both physical support and function evaluation.

The application discloses an animal cell adaptive tracking method and system based on deep learning, and belongs to the technical field of cell image analysis; a YOLOv10 deep learning model is combined with a Btrack adaptive tracking algorithm, animal cell samples are first dyed and microimage collection is carried out, a cell detection model is trained after image preprocessing, data labeling and augmentation, the model is converted into an ONNX format to realize efficient inference; then, Kalman filtering prediction and three-round Hungarian algorithm matching are carried out to complete cell adaptive tracking, the problems of cell occlusion, division, overlap and loss of tracking can be effectively handled, and stable and continuous cell motion trajectories are generated; the application can be deployed without GPU, has strong generalization ability, high detection and tracking precision, is suitable for scenes such as microecological research, clinical sample testing, drug effect evaluation and cell behavior analysis, and can significantly improve the automation level and stability of animal cell detection, counting and dynamic tracking.

The application relates to a preparation method and application of a Janus structure photothermal-piezoelectric hydrogel dressing for infected wound repair, and the preparation method comprises the following steps: phenylboronic acid grafted methyl acrylamide gelatin and dopamine grafted hyaluronic acid are respectively dissolved in deionized water, MXene@CeO2 heterojunction is uniformly ultrasonically dispersed, after mixing, sodium hydroxide is used to adjust the pH to be slightly alkaline, and a Janus structure inner layer is formed; phenylboronic acid grafted gelatin and ortho-nitrobenzyl alcohol grafted hyaluronic acid are respectively dissolved in deionized water, BaTiO3 is uniformly ultrasonically dispersed, and a Janus structure outer layer is formed after mixing. The Janus structure photothermal-piezoelectric hydrogel dressing prepared by the application has the characteristics of space-time release to adapt to the wound repair process, in addition, has excellent photothermal conversion performance, kills bacteria on the infected wound through photothermal heating, effectively simulates physiological electrical signals through piezoelectric effect, regulates cell behavior, and can be used for infected wound repair.

The application discloses a kind of corneal near physiological biomechanics intelligent simulation method and device, it is related to biomechanics and biomechanics experimental device technical field, the present application includes mutually connected force load cavity, force loading component, sensor monitoring feedback component etc..The present application can realize to corneal repair material, corneal cell, corneal cell-material compound loading individual and compound flow shear force, static pressure, dynamic pressure, static tension and dynamic tension, gas-liquid dynamic culture is simultaneously carried out, and through pressure and flow sensor, the stress size of loading is monitored in real time, reach accurate intelligent comprehensive simulation cornea in body environment.The scheme provided by the present application can be used for the mechanical property detection of corneal tissue and material, obtains the material attribute under near physiological state;It can also be used for the mechanical loading of cell, to observe the cell behavior and functional change after near physiological mechanical loading.

A method includes generating a first node of a directed tree. The first node digitally represents a first cell population of a cell line. The method further includes receiving an indication from a user of a protocol to be performed on the first cell population that will generate a second cell population of the cell line. The method further includes generating a second node of the directed tree after the user has performed the protocol on the first cell population to generate the second cell population. The second node is automatically assigned as (1) a child node of the first node and (2) digitally represents the second cell population. The first node is connected to the second node in the directed tree via an edge. The method further includes causing, data to be stored at the edge. The data is a transformation of the protocol used to generate the second cell population. This structured cell associated data can be used to train AI algorithms to predict how protocol steps affect cell behavior (cell morphology, viability, fitness, cell-cell interactions, protein / compound production, expression profiles among other things).

Provided herein are systems, devices, and methods for processing, analyzing, and classifying biological data sets and generation of cell profiles. The data sets may include multi-omic data. Some embodiments may include the use of machine learning in training a classifier of raw multi-omic data and incorporating system biology knowledge to understand cellular behavior and cell status at the biomolecular level.

The invention relates to a stimulus-responsive CRISPR-Cas12a (clustered regularly interspaced short palindromic repeats-associated 12a) system and application thereof, and belongs to the technical field of biology. The CRISPR-Cas12a (clustered regularly interspaced short palindromic repeats-CRISPR-Cas12a) system with the stimulation response comprises a DNA (deoxyribonucleic acid) tetrahedron-stimulation response factor aptamer compound and a CRISPR-Cas12a reaction system, the DNA tetrahedron-stimulus response factor aptamer compound is formed by hybridizing a DNA tetrahedron and a stimulus response factor aptamer; the DNA tetrahedron is formed by self-assembly of TH1, TH2, TH3 and a target chain TS of a CRISPR-Cas12a reaction system, and the stimuli response factor aptamer and the TS are hybridized so that the stimuli response factor and the stimuli response factor aptamer are combined to expose the TS under the action of the stimuli response factor, and then the CRISPR-Cas12a reaction system is activated. The H1 / H3 treated by the CRISPR-Cas12a with stimulation response can effectively inhibit the phosphorylation level of c-Met and the phosphorylation level of downstream protein of the c-Met, and inhibit the proliferation, migration and invasion ability of cells. The invention realizes specific control of membrane receptor dimerization and corresponding cell behaviors in a customizable manner.

The invention relates to medicine, biology, veterinary, pharmacology diagnostics, agriculture, ecology, meterology, seismology, construction biotechnology, biomanufacturing and provided herein are products and methods for managing cells behavior, memory of cells and erasure of cell memory. The present invention describes products and methods that, unlike the known ones, make it possible by treating them with an RNase, a DNase, an antibody that binds to an RNA and / or DNA and / or a molecule with nuclease activity to control the properties of cells and organisms without the use of mutagens and / or the special introduction of genes and / or use of specific gene tools and / or changing its environmental conditions.

The invention relates to medicine, biology, veterinary, pharmacology diagnostics, agriculture, ecology, meteorology, seismology, construction biotechnology, biomanufacturing and provided herein are products and methods for managing cells behavior, memory of cells and erasure of cell memory. The present invention describes products and methods that, unlike the known ones, make it possible to control the properties of cells and organisms without the use of mutagens and / or the special introduction of genes and / or use of specific gene tools and / or changing its environmental conditions.

The invention discloses a composition for promoting tissue regeneration and injury repair and application thereof. Experiments find that macrophages are more easily polarized to M2 under mechanical stimulation, meanwhile, molecules CCM3 on adhesion spots are reduced, further molecular dynamics simulation finds that combination of CCM3 protein and paxilin protein is reduced and combination of FAK protein and paxilin protein is increased under certain mechanical stimulation, and meanwhile, cytoskeleton rearrangement is obvious. The invention clarifies the molecular mechanism of the influence of mechanical stimulation on cell behavior, and thus can be used for developing a novel regeneration treatment method.

The invention provides construction and application of an intermolecular structural domain tension probe, the probe comprises a first structure, a second structure and a fluorescent protein pair eCFP and eYFP, the first structure and the second structure are respectively connected to two ends of the fluorescent protein pair, and the eCFP and the eYFP are connected through a short peptide; the first structure is a structural domain or protein capable of being combined with a cytoskeleton connecting protein, and the second structure is a structural domain or protein capable of being combined with a cytoskeleton; the cytoskeleton connecting protein is selected from talin, SYNE4, SYNE3, SYNE2, Desmoplakin and the like; the cytoskeleton is a microfilament, a microtubule or an intermediate filament. The intermolecular structural domain tension probe can be used for detecting intracellular mechanical signals, the limitation of a traditional tension probe is overcome, the adverse effect of function enhancement caused by gene cloning is avoided, a new opinion is provided for a mechanical transduction molecular mechanism, and the application has the advantages that the application range is wide, and the application prospect is wide. And a more accurate experimental tool is provided for the research of cell mechanics biology, and the effect of a mechanical signal in cell behavior regulation and control is disclosed.

The invention relates to a voltage-gated anion channel protein designed from de novo and application of the voltage-gated anion channel protein. The voltage-gated anion channel protein (dVGACs) designed from the beginning has a funnel-shaped pentamer structure, each monomer in the pentamer is composed of three transmembrane helixes, and the amino acid sequence of each monomer comprises SEQ ID No. 1 or a mutant thereof, at least one amino acid of the 43rd, 47th, 51th and 55th sites of the amino acid sequence of the mutant is mutated into arginine or aspartic acid. The dVGACs provided by the invention have selectivity to chloride ions, have voltage-responsive conformational change, and cause opening of a central pore channel at a membrane potential of about + 40 mV or + 20 mV and above. Due to the properties, the biosensor has a wide application prospect in the aspects of neurological disease treatment, cell behavior regulation and control, biosensor development and the like.

The invention relates to medicine, biology, veterinary, pharmacology diagnostics, agriculture, ecology, meteorology, seismology, construction biotechnology, biomanufacturing and provided herein are products and methods for managing cells behavior, memory of cells and erasure of cell memory. The present invention describes products and methods that, unlike the known ones, make it possible to control the properties of cells and organisms without the use of mutagens and / or the special introduction of genes and / or use of specific gene tools and / or changing its environmental conditions.

The application discloses a bone implant with a lampshade-expanding locking and self-energy-supplying coating and a preparation method thereof, and belongs to the medical implant field. The bone implant is designed according to the contraction structure of a lampshade, is in an elongated form below a martensite phase transition temperature, and is in an open lampshade form above an austenite phase transition temperature. The bone implant realizes self-adaptive expansion and surface locking in a marrow cavity by using the super-elasticity and shape memory effect of a memory alloy, and provides instant three-dimensional mechanical stability beyond traditional bone plates and intramedullary nails. The surface of the bone implant is coated with a functional gradient coating, which can actively capture biochemical energy and mechanical energy in the body, and intelligently and time-sequentially regulate growth factor release and cell behavior according to the biochemical energy and the mechanical energy, so that the continuous glucose metabolism and physiological activities in the body are directly converted into electric energy and control signals for driving bone healing, and energy self-provision and logic autonomy are realized.

The present application provides a computer-assisted method for distinguishing cell activity behaviors in a culture medium, which relates to the field of cell image processing technology and includes the following steps: obtaining a target microscopic image of a target cell at the current moment, and extracting the static features of the target cell in the target microscopic image; if the recognition confidence is lower than a first threshold, retrieving a historical image sequence of the area where the target cell is located; screening at least N candidate images in the historical image sequence, and obtaining temporal dynamic features of the target cell based on the candidate images. The temporal dynamic features include the motion trajectory, motion speed, morphological changes and periodic changes related to the activity behavior of the target cell within a certain time period; combining the temporal dynamic features with the static features to perform activity behavior matching to obtain the activity behavior of the target cell. The present application significantly improves the robustness and accuracy of cell behavior recognition in complex culture environments through the fusion of static and dynamic features.

The invention discloses an MXene (at) SA fiber electrode capable of reducing neural immune response as well as a preparation method and application of the MXene (at) SA fiber electrode. The preparation method comprises the following steps: step 1, concentrating a single-layer MXene dispersion liquid into MXene spinning slurry with the concentration of 90-110 mg / mL; 2, respectively injecting the MXene spinning slurry and the sodium alginate solution into an outer needle and an inner needle of a coaxial needle head device of a wet spinning machine, extruding, and enabling the MXene spinning slurry and the sodium alginate solution to sequentially pass through a 90-degree bent needle head and a polytetrafluoroethylene tube to enter a rotary coagulating bath to form MXene-coated SA fibers; and step 3, washing with an ethanol / aqueous solution, collecting, and drying to obtain the MXene (at) SA fiber electrode. The MXene (at) SA fiber electrode is used as a brain implantation electrode, neuroinflammatory response generated during implantation can be effectively reduced, the problem of tissue-electrode mismatching is avoided, cell behavior regulation is enhanced, and integration of neural electrodes is improved.

The invention discloses a patterned cell culture substrate for cell behavior regulation and a preparation and use method thereof. The preparation method comprises the following steps: preparing the patterned cell culture substrate on the inner surface of a conventional cell culture substrate through a patterning technology; the patterned cell culture substrate has a three-dimensional microgroove structure. The three-dimensional microgroove structure can provide various three-dimensional microenvironments for cell growth, so that the contact guide effect of cells is triggered, the cell behaviors are regulated and controlled, and a good cell behavior regulation and control effect is achieved. By adopting the laser patterning technology, the photoetching technology and the electron beam photoetching technology, the rapid manufacturing of the patterned cell culture base material and the manufacturing of the cell culture base material with high-precision and high-complexity micro-nano scale patterns can be realized. Meanwhile, the problem that the pattern stability is insufficient when the patterned cell culture substrate is prepared by adopting a chemical modification method is avoided.

The present application relates to the field of medical materials, in particular to an artificial ligament and a preparation method thereof.The artificial ligament provided by the present application comprises a gel layer-artificial ligament composite system by preparing a layer of PVA hydrogel on the surface of the woven artificial ligament in situ, which has biological activity and anti-creep performance, and is beneficial to improve the medium and long-term clinical prognosis of the woven artificial ligament.The artificial ligament provided by the present application can also adjust the rigidity, thickness and micro-topology of the gel layer under cyclic loading, thereby producing different biological activity regulation effects, and there is a balance point of rigidity and micro-topology, so that the cell ingrowth induction effect is best.The artificial ligament provided by the present application has simple composition, only relies on biophysical clues to regulate cell behavior, avoids the biological safety problems caused by complex biological chemical components, and can simultaneously realize the biological activity improvement and anti-creep performance improvement of the traditional PET woven artificial ligament.

The invention relates to medicine, biology, veterinary, pharmacology diagnostics, agriculture, ecology, meterology, seismology, construction biotechnology, biomanufacturing and provided herein are products and methods for managing cells behavior, memory of cells and erasure of cell memory. The present invention describes products and methods that, unlike the known ones, make it possible by treating them with an RNase, a DNase, an antibody that binds to an RNA and / or DNA and / or a molecule with nuclease activity to control the properties of cells and organisms without the use of mutagens and / or the special introduction of genes and / or use of specific gene tools and / or changing its environmental conditions.

A 3D perfusion biochip system and method for more accurate modeling of in vivo environmental conditions and cellular behaviors. The biochip includes a cavity for containing culture media and cells. A plurality of series-coupled capillary tubes cross the cavity. In some embodiments, the capillary tubes are arranged in a swirl pattern. In some embodiments, the capillary tubes include micropores. In some embodiments, the biochip includes multiple parallel layers of series-connected capillary tubes, where the capillary tubes of each layer are independently arranged in one of an outside-in swirl pattern, an inside-out swirl pattern, or an S pattern.

The invention provides gradient bionic hydrogel for osteochondral regeneration as well as a preparation method and application of the gradient bionic hydrogel. According to the invention, through organic-inorganic co-assembly of functionalized HA and CPO, the gradient bionic hydrogel (BiGOH) with a space nano structure and a component gradient is constructed. According to the gel, biomimetic mineralization of CPO under the concentration gradient can be achieved, hydroxyapatite (HAP) nanocrystals are distributed in space, and the length of the hydroxyapatite nanocrystals is gradually increased to 117.6 + / -24.73 nanometers from 23.09 + / -7.35 nanometers. The bionic gradient structure of BiGOH can spatially program recruitment of stem cells and macrophages so as to establish a regeneration-promoting microenvironment. The cell behavior can be spatially regulated and controlled through the component constructed by the bionic non-classical crystallized cluster precursor and the nanostructure gradient, so that a regeneration strategy with a very prospect is provided for repairing osteochondral defects.

The application relates to the technical field of medical image analysis, in particular to a CAR-T cell culture monitoring system based on image recognition, which comprises a cell region division module, a cell morphology analysis module, a cell behavior dynamic analysis module and a cell population cooperative monitoring module.In the application, cell image gradient intensity analysis is carried out, cell edge detection is refined, and a segmentation threshold is dynamically adjusted, so that cell boundary extraction is ensured to be high in precision, noise interference is avoided, cell shape, texture and geometric features are refined, the recognition degree of morphological features is improved, cell morphology micro changes are accurately captured, cell behavior is dynamically tracked and trend evaluation is carried out based on adjacent frame images, and the dynamic changes such as cell division, aggregation and migration are accurately reflected, over-smoothing in a dynamic process is avoided, cell population behavior monitoring is combined with interaction and corresponding speed calculation, population cooperative effect change trends are comprehensively evaluated, and more refined cell culture environment optimization and clinical research support are provided.

The application belongs to the field of medical modified and natural polymer hydrogel, and provides a viscoelastic hydrogel with adjustable viscosity and application thereof in evaluation of influence of hydrogel viscosity on cell behavior. Through a double crosslinking mechanism of covalent crosslinking and ionic crosslinking, two viscoelastic hydrogels with the same initial modulus but different viscosities are prepared. The covalent crosslinking hydrogel is composed of methacrylated alginate (AlgMA), gelatin (Gel) and lithium phenyl (2,4,6-trimethylbenzoyl) phosphate (LAP), and the ionic crosslinking hydrogel is composed of sodium alginate (Alg), gelatin (Gel) and calcium chloride. The application realizes fine adjustment of the viscosity of the hydrogel without changing the initial modulus by precisely controlling the crosslinking degree, which has an important influence on neural stem cell adhesion and survival. The experimental method provided by the application verifies the key role of the viscosity of the viscoelastic matrix in the regulation of cell behavior.

The invention belongs to the cross technical field of biotechnology and biomedical engineering, and relates to a method and a device for detecting protein interaction in living cells, in particular to a method and a device for in-situ detection of protein-protein interaction (PPIs) in living cells at a single cell level and real-time correlation analysis of the PPIs and cell behaviors (such as migration and proliferation). The invention firstly provides a micro-fluidic chip system for interaction detection and real-time behavior monitoring of proteins in living cells. The micro-fluidic chip system comprises a micro-fluidic chip and a detection probe group reagent, the micro-fluidic chip comprises a single cell capture layer (100), a nano-film (200), a loading chamber layer (300) and an electrode layer (400). According to the invention, on the same chip platform, integration of single cell capture, molecule delivery, PPI detection, behavior monitoring, mechanism analysis and whole process is realized.

A 3D perfusion bioreactor system and method for more accurate modeling of in vivo environmental conditions and cellular behaviors. In some embodiments, the bioreactor includes a cavity for containing culture media and cells. A plurality of capillary tubes cross the cavity. Artery capillary tubes may transport culture media received from an inlet of the bioreactor, across the cavity, and into a return compartment. Vein capillary tubes may transport culture media from the return compartment, across the cavity, and to an outlet of the bioreactor. Metabolites may diffuse from the capillary tubes into the cavity for cellular consumption, and metabolic waste may diffuse from the cavity into the capillary tubes for removal from the bioreactor. In some embodiments, culture media discharged from the outlet may be processed via a waste treatment device and returned to the inlet.

The present invention discloses a device and method for generating dynamic chemical signals using a piezoelectric diaphragm pump, aiming to improve the accuracy and efficiency of drug dynamic signal generation in cell biology research; the device is composed of a dynamic chemical signal generation component and a microfluidic mixing chip, wherein the dynamic chemical signal generation component includes a piezoelectric diaphragm pump, a piezoelectric diaphragm pump control module and a piezoelectric diaphragm pump control module rack; the piezoelectric diaphragm pump can achieve precise flow control, and supports multiple pumps in parallel or series to increase flow; the microfluidic mixing chip is provided with multiple liquid inlets, and uniform mixing is achieved through multiple rows of mixing channels; the present invention can fully automatically control the pumping of liquids, reducing manual operations. The overall design of the present invention is compact and portable, suitable for desktop use, and provides convenience for cell experiments. The present invention can effectively generate single or complex dynamic chemical signals, accurately simulate the cell microenvironment, and is of great significance to the study of cell behavior.

The application provides a preparation method of a gradient fiber membrane and the gradient fiber membrane. The preparation method comprises the following steps: electrospinning a high-molecular polymer solution, and then soaking the obtained electrospinning nanofiber membrane in a cation solution to clean the nanofiber membrane, and then soaking the cleaned nanofiber membrane in an anion solution to obtain a primary self-assembled fiber membrane; the liquid level of the cation solution and the anion solution is kept unchanged during the soaking, and the liquid level is continuously increased; the above operation is repeated, and after n times, an n-layer self-assembled gradient fiber membrane is obtained, and n is greater than or equal to 3. The gradient fiber membrane prepared by the application can keep good morphology, porosity and good orientation; the self-assembled layer number is increased, and more bioactive substances can be adsorbed on the nanofiber; the fiber membrane has extremely strong hydrophilicity, extremely high cell activity, good biocompatibility, and the cell behavior can be well controlled by designing a gradient structure of bioactive components on the electrospinning nanofiber, so that the cell migration is promoted.

The invention belongs to the field of biological materials and biomedicine, and discloses a preparation method of an integrin decoy receptor based on molecular imprinting and application of the integrin decoy receptor in bidirectional regulation of integrin signals. From the perspective of design of an integrin bait receptor, the bait receptor with a similar artificial antagonistic molecule molecular function is prepared through a solid-phase template imprinting technology by taking bidirectional regulation (inhibition and excitation) of integrin-related cell behaviors as a target, and the bait receptor is used for regulating and controlling integrin-mediated cell behaviors. The method is crucial for replacing an antagonist to realize bidirectional regulation and control of integrin signals. The bait receptor prepared by the invention can be reversibly combined with integrin targeting polypeptide, so that the polarization of macrophages related to integrin and the differentiation behavior of mesenchymal stem cells are bidirectionally regulated and controlled. The invention proves the feasibility of the molecularly imprinted decoy receptor in dynamic signal regulation and cell operation, and shows a huge prospect of customized therapeutic drugs in tissue repair and other medical fields.

The invention belongs to the field of material science and medicine, and relates to a preparation method and application of three-dimensional cell culture hydrogel capable of performing molecular-level accurate force stimulation on cells. The preparation method comprises the following steps: 1) respectively dissolving a polypeptide cross-linking agent and force application molecules in a first buffer solution to obtain a polypeptide cross-linking agent mixed solution and a force application molecule mixed solution; 2) dissolving the methacrylated glucan in a second buffer solution to obtain a methacrylated glucan mixed solution; and 3) fully reacting the force application molecule mixed solution with the methacrylated glucan mixed solution, and mixing with the polypeptide cross-linking agent mixed solution to obtain the three-dimensional cell culture hydrogel capable of performing molecular-level accurate force stimulation on cells. The hydrogel is good in biocompatibility and free of obvious cytotoxicity, and force application molecules can stimulate cells for a long time; the photoreaction is quick, the spatial-temporal resolution is realized, the cell behavior can be regulated and controlled in situ, the cell spreading and migration are promoted, and a real in-vivo mechanical environment is provided.