33 results about "Cell behaviour" patented technology

The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. In some preferred aspects, the assays are designed to investigate the affects of compounds on cells, such as cytotoxicity assays. In other preferred aspects, the assays are designed to investigate the compounds that effect IgE-mediated responses of cells to antigens.

Surfaces, kits, and methods for the modulation of cell behavior in vitro by patterned nanoscale topography. The invention is particularly useful for providing means to affect and control the growth and differentiation of human embryonic stem cells.

The invention relates to the technical field of surface modification of biomedical polymer materials, and in particular, relates to a method for preparing a multi-scale porous structure on the surface of polyether-ether-ketone, wherein the method comprises the steps: putting polyether-ether-ketone in an ultrasonic field, carrying out sulfonation treatment, and forming the multi-scale porous structure on the surface of polyether-ether-ketone. 100-2000 nm small-scale holes are generated through sulfonation, and cell behaviors can be regulated and controlled; and large-scale holes (50-250 [mu]m) generated by ultrasonic cavitation allow tissue and blood vessels to grow in, and the combination of the small-scale holes and the large-scale holes allows the tissue and the blood vessels to grow in and also can regulate and control cell behaviors.

This disclosure provides for a three-dimensional (3D) microenvironment presenting defined physical or mechanical cues that regulate cellular behavior and use of the matrix. The disclosure also provides for devices and methods for screening for optimal combinations of physical and mechanical cues in order to create a microenvironment that can regulate specific cellular behavior such as cell growth, proliferation, migration or differentiation.

The invention relates to an orientation material based on magnetic nanofibers and a preparation method and an application of the orientation material based on the magnetic nanofibers, a magnetic nanomaterial and a nanofiber material are combined, a mixed solution is cross-linked in a magnetic field and is induced by the magnetic field to form orientation gel, and the orientation material based on the magnetic nanofibers is obtained. The magnetic field is utilized to pretreat the magnetic nanofibers in the gel forming matrix to form the preset orientation structure, then the gel forming matrix is crosslinked and cured to fix the preset orientation structure, the prepared orientation material can be used for studying the relationship between material properties and cell behaviors under the nanometer-micrometer-macroscopic scale, and the regulation and control mechanism of the material on cell behaviors is disclosed.

The invention discloses a concentration gradient generation device for gel 3D cell culture. The device comprises multi-stage mixing and concentration gradient generation modules and gel chambers of multi-stage concentration gradient ranges, wherein a plurality of gel samples can be carried simultaneously; through application of sectional concentration gradients, detection and evaluation on cell behaviors in gel within different concentration gradient ranges can be performed simultaneously; and through setup of the multi-stage concentration gradient ranges, samples of different concentration spans can be simultaneously detected on a same fluid chip. The fluid chip provided by the invention has the advantages of being small in volume, low in cost, good in integration with a trace amount of gel, and the like, and has wide application prospects in aspects such as cell physiology, pathology, drug screening, biomedicines and environmental monitoring. Meanwhile, the invention further discloses a preparation method and an application method of the concentration gradient generation device.

A method for measuring surface-induced cellular behavior that includes one or more lithographically patterned, functionalizable structures on a substrate, for example gold islands or grooved quartz, in contact with a fluid and in registry with at least one living cell for a plurality of times. The structures' shape, height, pitch and ordering are controlled by the lithographic process, such that the physical cues imparted to the cell by topography can be tuned independently of the chemical biofunctionality which is subsequently imparted via surface chemistry. Cellular behavior data, such as adhesion, migration, differentiation, division, secretion, apoptosis and necrosis, is measured using imaging sensors in relation to the surface topography and surface chemistry for a plurality of times.

This disclosure provides for a three-dimensional (3D) microenvironment presenting defined physical or mechanical cues that regulate cellular behavior and use of the matrix. The disclosure also provides for devices and methods for screening for optimal combinations of physical and mechanical cues in order to create a microenvironment that can regulate specific cellular behavior such as cell growth, proliferation, migration or differentiation.

The invention discloses a cell communication analysis method and system. The method comprises cell communication prediction and ligand-target gene regulation prediction. Cell communication predictioncomprises analysis of expression abundance of a ligand-receptor pair, analysis of the number of ligand-receptor pairs, analysis of the number of significantly enriched ligand-receptor pairs and construction of a cell interaction network diagram. The ligand-target gene regulation and control prediction comprises ligand activity analysis and ligand-target gene regulation and control potential analysis; the cell communication analysis method mainly comprises the following steps: describing an incidence relation among cells, constructing a cell communication network by utilizing CellphoneDB software based on a single-cell gene expression quantity matrix, and analyzing ligand activity and the regulation potential of a ligand on a target gene by utilizing NicheNet software based on a ligand-target gene expression relation. Various graphical results are displayed, so that the incidence relation between cells is convenient to research; a new basic scientific research idea can be provided for revealing a certain cell behavior and deeply analyzing a cell communication result.

The present disclosure relates to a three-dimensional micro-environment structure for controlling cell behavior, a three-dimensional surface and an array for controlling cell behavior, and a method for manufacturing the three-dimensional micro-environment structure.

The invention related to a method of imaging a clonal cell line comprising providing a test animal comprising a marker gene, inducing inheritable activation of said marker in at least one cell of said test animal, wherein inheritable activation is induced in fewer than 1 in 27 cells in the tissue of interest, incubating the test animal, and visualising those clonal cells which express the marker gene as a result of the inheritable activation. In particular the invention concerns-methods where the tissue is epidermis, and wherein the visualisation is by confocal microscopy such as wholemount confocal microscopy. The invention also relates to toxicity and carcinogenicity testing using such methods.

The present invention provides a polypeptides capable of modulating tissue transglutarmnase-induced cell behaviour wherein the polypeptide comprises or consists of either (a) the amino acid sequence of a heparin-binding site of a tissue transglutaminase, or a functional fragment, variant, fusion or derivative thereof, or a fusion of said fragment, variant or derivative thereof or (b) an antibody capable of binding to a heparin-binding site of a lissue transglutaminase, or an antigen-binding fragment or derivative thereof. In one embodiment, the heparin-binding site of a tissue transglutaminase comprises or consists of an amino acid sequence of SEQ ID NO: 1, The invention further provides medical uses of the polypeptides of the invention and methods of treatment using the same.

A highly specific and beneficial T cell therapy can be provided by controlled ex vivo induction or amplification to produce antigen-specific immune cells (like T cells). The invention provides T cell manufacturing methods and therapeutic T cell compositions, for example, for treating subjects with cancer and other conditions, diseases and disorders, as well as individual antigen-specific T cell therapies.

The invention provides a nerve regulating and controlling method and a nerve regulating and controlling device, and relates to the technical field of medical equipment. The nerve regulating and controlling device is used for overcoming the defects of the nerve regulating and controlling device in the prior art, and comprises a brain slice fixing device, an electric stimulation device and a collecting device, wherein an in-vitro live brain slice is fixed in the brain slice fixing device; the brain slice fixing device is filled with circulating cerebrospinal fluid meeting the preset temperature,and a cerebrospinal fluid loop is formed in the brain slice fixing device; the in-vitro live brain slice is obtained by transfecting a brain area interested in a receptor and according to a preset mode; the electric stimulation device is connected with the brain slice fixing device and is used for applying stimulation to the in-vitro live brain slice in the brain slice fixing device; and the collecting device is used for showing the ethological and morphological change of a lentivirus transfection marked cell or the change of ions in an organelle and a cytoplasma of a cell in real time in theprocess of applying stimulation to the in-vitro live brain slice by the electric stimulation device.

The invention discloses a method for thermally stimulating cultured cells among cells. Firstly, temperature of a water bath is set to be experimental temperature; the water bath is started for heating; when the temperature of the water bath reaches the final experimental temperature, a cell culture plate / flask holding cells which need to be thermally stimulated and a cell culture plate / flask as blank control are respectively placed at slot positions of 2 base frames; the lower part of the cell culture plate / flask is submerged in water, and the upper part of the cell culture plate / flask is opened and is exposed above a water surface; temperature of the inner surface of the cell culture plate / flask as the blank control is monitored by an infrared temperature monitoring system; heating is performed for temperature reaching time, and then heating stimulation is performed for time required for an experiment; and after completion, the cell culture plate / flask holding the cultured cells is quickly taken out, and thus, the precise and instantaneous thermal stimulation experiment of the cultured cells is completed. The method can very well realize precise and instantaneous thermal stimulation treatment on the cultured cells, and can very well meet the experimental needs like accurately observing the influence of thermal stimulation on cell behaviors and functions.

The invention discloses a three-dimensional annular cytoskeleton in response to radio stimulation and a preparation method and application thereof. The preparation method comprises the following steps: growing graphene on a copper / nickel template by adopting a chemical vapor deposition method to prepare a three-dimensional annular cytoskeleton; or blending a conductive substance and a fiber to form a conductive fiber, and then performing treatment by adopting a template method to prepare the three-dimensional annular cytoskeleton; or printing conductive hydrogel and / or conductive aerogel by adopting a 3D printing method to prepare the three-dimensional annular cytoskeleton. The prepared three-dimensional annular cytoskeleton is accurate and controllable in size and good in conductivity; and meanwhile the three-dimensional annular cytoskeleton inoculated with the mesenchymal stem cells by using radio stimulation is simple and convenient to use, the trouble of wire connection is solved,the problems of intra-tissue flowing and low survival rate of the mesenchymal stem cells and cell transplantation injury are reduced, and cell behaviors can be controlled.

The invention relates to an anisotropic micro-trend structure material, a preparation method thereof and an application of the material. The anisotropic micro-trend structure material comprises a substrate, at least one surface of the substrate is provided with a projecting micro-structure, a cell adhesion resistant interface layer is further arranged on the surface of the substrate, and a chromium layer, a gold nanoparticle layer and a cell adhesion promoting layer are sequentially arranged on the same side of the cell adhesion resistant interface layer of each projecting micro-structure. Cell adhesion resistant PEG (polyethylene glycol) molecular chains are modified on a PDMS (polydimethylsiloxane) surface to obtain a cell adhesion resistant interface, the gold nanoparticle layer is deposited on one side of a micro-column in an inclined evaporation mode, and RGD (arginine-glycine-aspartic acid) polypeptides promoting cell adhesion are modified to a gold-plated surface to finally obtain an anisotropic cell adhesion micro-trend structure. The structure has the functions of inducing and changing cell migration and has excellent research significance and application values for drug-stimulation-free cell behaviors, research for migration behavior mechanisms of various cells and regulation of diffusion of tumor cells in an organism.

The invention provides a quantitative characterization method of cell membrane tension, which combines a cell membrane tension model and a cytoplasm viscoelasticity model, and performs quantitative characterization on the cell membrane tension by using an atomic force microscope. The method specifically comprises the following steps: (1) obtaining single cell force relaxation signal data based on an AFM nanoindentation experiment; (2) constructing a cell power relaxation curve equation; and (3) quantitative calculation of cell membrane tension. The detection means for quantitatively detecting the membrane tension of the living cells, provided by the invention, is helpful for people to have further deeper understanding on cell behaviors, and also provides a new view angle and direction for people to carry out early diagnosis, regulation and treatment on various diseases by regulating the physical characteristics of the cells.

The invention provides materials presenting a biologically active matrix comprising a physiological fibrillar fibronectin network, such as implantable constructs, and their use for modulating cell behaviour and fate, including cell growth, proliferation and / or differentiation, such as for promoting tissue regeneration, for example, bone regeneration or vascularization. Also provided are constructs presenting a biologically active matrix comprising a physiological fibrillar fibronectin network for sustaining growth of stem cells or maintaining stem cells (maintaining stemness).

The invention relates to a single-layer parallel fiber pattern with a cell adhesion contrast characteristic and a preparation method thereof. The fiber pattern comprises a substrate and single-layer parallel fibers fixed on the substrate, wherein two ends of the fibers are fixed on the substrate through an adhesive; and the surface of the substrate is grafted and modified by an anti-cell adhesion grafting reagent. The preparation method comprises the following steps of: (1) after grafting and modifying the substrate by adopting the anti-cell adhesion grafting reagent, preparing the single-layer parallel fibers on the surface of the substrate by virtue of an electrostatic spinning, dry spinning, wet spinning or 3D printing method; and (2) coating the adhesive at the combined parts of the two ends of the single-layer parallel fibers prepared in the step (1) and the substrate, and crosslinking and curing the adhesive in a heating curing or illumination curing manner. The fiber pattern prepared by the invention can effectively eliminate interference of various background adhesions, and an effective material platform is provided for accurately revealing the influence of the characteristics such as diameter, material and density arrangement of the fibers on cell behaviors.

Disclosed are methods for cell transfection and regulating cellular behavior. More particularly, the present disclosure relates to methods of non-viral cell transfection and regulating cellular behavior using mineral coatings that allow for the enhanced transfection of cells. The mineral coatings bind polynucleotides and provide a source of calcium and phosphate ions to enhance transfection. The present disclosure also provides a high throughput platform for screening non-viral transfection of cells. The methods of the present disclosure also provide an advantageous polynucleotide delivery platform because the mineral coatings may be deposited on various medical device materials after being specifically developed using the high throughput screening platform.

The present disclosure relates to the technical field of surface modification of biomedical polymer materials, in particular to a method for preparing a multi-scale porous structure on the surface of polyetheretherketone, comprising: placing polyetheretherketone in an ultrasonic field for sulfonation treatment, The polyetheretherketone surface forms a multi-scale porous structure. Sulfonation produces small-scale pores of 100-2000 nm, which can regulate cell behavior; ultrasonic cavitation produces large-scale pores (50-250 μm), which allow tissue and blood vessel ingrowth. Cell behavior is regulated.

The disclosure provides a device for developing organized tissue strips, such as cardiac tissue strips, for high-throughput assays of functional performance and the methods involved in fabricating, assembling, implementing, utilizing and analyzing data from such assays. The disclosure further provides systems for constructing such devices, systems comprising those devices comprising cells and extracellular matrix material for developing organized tissue strips or comprising the devices and organized tissue strips. The disclosure further provides methods for assaying a property of a tissue strip, such as contractile force.

The invention discloses a photopolymerizable gel with color self-feedback hardness distribution and a preparation method thereof. The gel is used for cell supports, and the preparation steps of the gel are as follows: preparing a prepolymerization solution; pouring the prepolymerization solution into two The glass slides placed in parallel were irradiated with 365 nm ultraviolet light to polymerize; during the process, 254 nm and 365 nm ultraviolet light could be used to regulate the mechanical properties of the gel to obtain cell scaffolds with regionalized distribution of mechanical properties. The hardness and distribution of the modified cell scaffold will be displayed in the form of color, and the hardness distribution on the scaffold can be read in situ by taking pictures. This method is applied to a variety of cell culture systems to control the properties of the microenvironment in real time and guide the behavior of cells on it in situ. Without opening the chip and without additional detection equipment, the mechanical parameters of the regulated cell scaffold and the relationship between cell behavior and scaffold properties can be directly obtained through color analysis, which has great application potential.

The invention discloses a composition and a method for generating a linear polymer through light-operated RAFT polymerization in living cells. A monomer, a chain transfer agent, a photoinitiator and living cells are incubated together to achieve uptake of the composition by the cells, and then in-situ synthesis of the polymer in the cells is achieved through illumination. The polymer synthesized in the cells is further extracted, represented, tested and compared with a polymer obtained through in-vitro simulation, and it is found that the polymer synthesized in the cells has high molecular weight and narrow molecular weight distribution and belongs to typical polymer products with controllable polymerization. Therefore, the purpose of introducing the unnatural polymer with a controllable structure into the living cell can be achieved, the composition and the structure in the cell can be further artificially regulated, and even the purpose of controlling the cell behavior and the cell function can be possibly achieved.

Described is a three-dimensional (3D) microenvironment presenting defined biochemical and physical cues that regulate cellular behavior and use of the microenvironment. A composition to form the 3D microenvironment is provided by combining one or more natural or synthetic polymeric materials and substrate proteins recombinantly or chemically functionalized with a variety of bioactive peptides such as extracellular matrix-derived or growth factor-derived peptides. Also described are devices and methods for screening for optimal combinations of the bioactive motifs in order to create an extracellular microenvironment that can regulate specific cellular behavior such as cell growth, proliferation, migration or differentiation.

The invention relates to the field of biochips and in particular relates to a micro-fluidic chip for cell culture as well as a preparation method and application of the cell culture. The micro-fluidic chip comprises a cell culture layer (1), a substrate layer (2) and a clamping layer (3); materials required for preparation of the micro-fluidic chip are conventional slides in a laboratory and PDMS (Polydimethylsiloxane); and the micro-fluidic chip is prepared by using the five steps of arranging the slides, preparing a PDMS preforming solution, molding, forming and packaging. The micro-fluidic chip is simple to manufacture and low in manufacturing cost; and the micro-fluidic chip can be manufactured without the assistance of expensive instruments. The microfluidic chip provided by the invention can be used for realizing multiple modes of cell culture, accurately controlling the parameters of cells in a microenvironment by a smart configuration design and a microfluidic control technology and investigating the influence of the cell microenvironment on cell behaviors.

The invention discloses a three-dimensional graphene foam / natural polysaccharide-based hydrogel composite scaffold and a preparation method thereof. The composite scaffold includes: three-dimensional graphene foam, and a natural polysaccharide-based hydrogel combined with the three-dimensional graphene foam. The three-dimensional graphene foam / natural polysaccharide-based hydrogel composite scaffold of the present invention has both the advantages of graphene and hydrogel, and the natural polysaccharide-based hydrogel also significantly improves the toughness of the three-dimensional graphene foam, and makes the composite scaffold It has good electrical conductivity, and when it is applied to cell culture, it can make cells adhere and spread, and can regulate cell behavior through chemically bonded biological factors.

The invention discloses a concentration gradient generation device for gel 3D cell culture, which includes a multi-stage mixing and concentration gradient generation module, and a gel chamber with a multi-stage concentration gradient range, which can simultaneously load multiple gel samples , and through the application of segmental concentration gradients, the detection and evaluation of cell behavior in gels in different concentration gradient ranges is carried out at the same time; the establishment of multi-level concentration gradient ranges can simultaneously detect cells in different concentrations on the same fluid chip. span samples; the fluid chip provided by the present invention has the advantages of small size, low cost, and good integration performance with microgels, and has broad applications in in vitro tissue reconstruction, cell physiology, pathology, and drug screening, biomedicine, and environmental monitoring. Application prospect; at the same time, the invention also discloses its preparation method and application method.