33 results about "Cell behaviour" patented technology

The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. The methods can be used to test the effects of compounds on cells, such as in cytotoxicity assays. Methods of cytotoxicity profiling of compounds are also provided.

This disclosure provides for a three-dimensional (3D) microenvironment presenting defined physical or mechanical cues that regulate cellular behavior and use of the matrix. The disclosure also provides for devices and methods for screening for optimal combinations of physical and mechanical cues in order to create a microenvironment that can regulate specific cellular behavior such as cell growth, proliferation, migration or differentiation.

The present disclosure relates to the technical field of surface modification of biomedical polymer materials, in particular to a method for preparing a multi-scale porous structure on the surface of polyetheretherketone, comprising: placing polyetheretherketone in an ultrasonic field for sulfonation treatment, The polyetheretherketone surface forms a multi-scale porous structure. Sulfonation produces small-scale pores of 100-2000 nm, which can regulate cell behavior; ultrasonic cavitation produces large-scale pores (50-250 μm), which allow tissue and blood vessel ingrowth. Cell behavior is regulated.

The invention discloses a photopolymerizable gel with color self-feedback hardness distribution and a preparation method thereof. The gel is used for cell supports, and the preparation steps of the gel are as follows: preparing a prepolymerization solution; pouring the prepolymerization solution into two The glass slides placed in parallel were irradiated with 365 nm ultraviolet light to polymerize; during the process, 254 nm and 365 nm ultraviolet light could be used to regulate the mechanical properties of the gel to obtain cell scaffolds with regionalized distribution of mechanical properties. The hardness and distribution of the modified cell scaffold will be displayed in the form of color, and the hardness distribution on the scaffold can be read in situ by taking pictures. This method is applied to a variety of cell culture systems to control the properties of the microenvironment in real time and guide the behavior of cells on it in situ. Without opening the chip and without additional detection equipment, the mechanical parameters of the regulated cell scaffold and the relationship between cell behavior and scaffold properties can be directly obtained through color analysis, which has great application potential.

The invention discloses a three-dimensional graphene foam / natural polysaccharide-based hydrogel composite scaffold and a preparation method thereof. The composite scaffold includes: three-dimensional graphene foam, and a natural polysaccharide-based hydrogel combined with the three-dimensional graphene foam. The three-dimensional graphene foam / natural polysaccharide-based hydrogel composite scaffold of the present invention has both the advantages of graphene and hydrogel, and the natural polysaccharide-based hydrogel also significantly improves the toughness of the three-dimensional graphene foam, and makes the composite scaffold It has good electrical conductivity, and when it is applied to cell culture, it can make cells adhere and spread, and can regulate cell behavior through chemically bonded biological factors.

The invention discloses a concentration gradient generation device for gel 3D cell culture, which includes a multi-stage mixing and concentration gradient generation module, and a gel chamber with a multi-stage concentration gradient range, which can simultaneously load multiple gel samples , and through the application of segmental concentration gradients, the detection and evaluation of cell behavior in gels in different concentration gradient ranges is carried out at the same time; the establishment of multi-level concentration gradient ranges can simultaneously detect cells in different concentrations on the same fluid chip. span samples; the fluid chip provided by the present invention has the advantages of small size, low cost, and good integration performance with microgels, and has broad applications in in vitro tissue reconstruction, cell physiology, pathology, and drug screening, biomedicine, and environmental monitoring. Application prospect; at the same time, the invention also discloses its preparation method and application method.