33 results about "Cell behaviour" patented technology

The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. In some preferred aspects, the assays are designed to investigate the affects of compounds on cells, such as cytotoxicity assays. In other preferred aspects, the assays are designed to investigate the compounds that effect IgE-mediated responses of cells to antigens.

The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. The methods can be used to test the effects of compounds on cells, such as in cytotoxicity assays. Methods of cytotoxicity profiling of compounds are also provided.

The invention relates to the technical field of surface modification of biomedical polymer materials, and in particular, relates to a method for preparing a multi-scale porous structure on the surface of polyether-ether-ketone, wherein the method comprises the steps: putting polyether-ether-ketone in an ultrasonic field, carrying out sulfonation treatment, and forming the multi-scale porous structure on the surface of polyether-ether-ketone. 100-2000 nm small-scale holes are generated through sulfonation, and cell behaviors can be regulated and controlled; and large-scale holes (50-250 [mu]m) generated by ultrasonic cavitation allow tissue and blood vessels to grow in, and the combination of the small-scale holes and the large-scale holes allows the tissue and the blood vessels to grow in and also can regulate and control cell behaviors.

This disclosure provides for a three-dimensional (3D) microenvironment presenting defined physical or mechanical cues that regulate cellular behavior and use of the matrix. The disclosure also provides for devices and methods for screening for optimal combinations of physical and mechanical cues in order to create a microenvironment that can regulate specific cellular behavior such as cell growth, proliferation, migration or differentiation.

The invention provides a nerve regulating and controlling method and a nerve regulating and controlling device, and relates to the technical field of medical equipment. The nerve regulating and controlling device is used for overcoming the defects of the nerve regulating and controlling device in the prior art, and comprises a brain slice fixing device, an electric stimulation device and a collecting device, wherein an in-vitro live brain slice is fixed in the brain slice fixing device; the brain slice fixing device is filled with circulating cerebrospinal fluid meeting the preset temperature,and a cerebrospinal fluid loop is formed in the brain slice fixing device; the in-vitro live brain slice is obtained by transfecting a brain area interested in a receptor and according to a preset mode; the electric stimulation device is connected with the brain slice fixing device and is used for applying stimulation to the in-vitro live brain slice in the brain slice fixing device; and the collecting device is used for showing the ethological and morphological change of a lentivirus transfection marked cell or the change of ions in an organelle and a cytoplasma of a cell in real time in theprocess of applying stimulation to the in-vitro live brain slice by the electric stimulation device.

The present disclosure relates to the technical field of surface modification of biomedical polymer materials, in particular to a method for preparing a multi-scale porous structure on the surface of polyetheretherketone, comprising: placing polyetheretherketone in an ultrasonic field for sulfonation treatment, The polyetheretherketone surface forms a multi-scale porous structure. Sulfonation produces small-scale pores of 100-2000 nm, which can regulate cell behavior; ultrasonic cavitation produces large-scale pores (50-250 μm), which allow tissue and blood vessel ingrowth. Cell behavior is regulated.

The invention discloses a photopolymerizable gel with color self-feedback hardness distribution and a preparation method thereof. The gel is used for cell supports, and the preparation steps of the gel are as follows: preparing a prepolymerization solution; pouring the prepolymerization solution into two The glass slides placed in parallel were irradiated with 365 nm ultraviolet light to polymerize; during the process, 254 nm and 365 nm ultraviolet light could be used to regulate the mechanical properties of the gel to obtain cell scaffolds with regionalized distribution of mechanical properties. The hardness and distribution of the modified cell scaffold will be displayed in the form of color, and the hardness distribution on the scaffold can be read in situ by taking pictures. This method is applied to a variety of cell culture systems to control the properties of the microenvironment in real time and guide the behavior of cells on it in situ. Without opening the chip and without additional detection equipment, the mechanical parameters of the regulated cell scaffold and the relationship between cell behavior and scaffold properties can be directly obtained through color analysis, which has great application potential.

Described is a three-dimensional (3D) microenvironment presenting defined biochemical and physical cues that regulate cellular behavior and use of the microenvironment. A composition to form the 3D microenvironment is provided by combining one or more natural or synthetic polymeric materials and substrate proteins recombinantly or chemically functionalized with a variety of bioactive peptides such as extracellular matrix-derived or growth factor-derived peptides. Also described are devices and methods for screening for optimal combinations of the bioactive motifs in order to create an extracellular microenvironment that can regulate specific cellular behavior such as cell growth, proliferation, migration or differentiation.

The invention relates to the field of biochips and in particular relates to a micro-fluidic chip for cell culture as well as a preparation method and application of the cell culture. The micro-fluidic chip comprises a cell culture layer (1), a substrate layer (2) and a clamping layer (3); materials required for preparation of the micro-fluidic chip are conventional slides in a laboratory and PDMS (Polydimethylsiloxane); and the micro-fluidic chip is prepared by using the five steps of arranging the slides, preparing a PDMS preforming solution, molding, forming and packaging. The micro-fluidic chip is simple to manufacture and low in manufacturing cost; and the micro-fluidic chip can be manufactured without the assistance of expensive instruments. The microfluidic chip provided by the invention can be used for realizing multiple modes of cell culture, accurately controlling the parameters of cells in a microenvironment by a smart configuration design and a microfluidic control technology and investigating the influence of the cell microenvironment on cell behaviors.

The invention discloses a three-dimensional graphene foam / natural polysaccharide-based hydrogel composite scaffold and a preparation method thereof. The composite scaffold includes: three-dimensional graphene foam, and a natural polysaccharide-based hydrogel combined with the three-dimensional graphene foam. The three-dimensional graphene foam / natural polysaccharide-based hydrogel composite scaffold of the present invention has both the advantages of graphene and hydrogel, and the natural polysaccharide-based hydrogel also significantly improves the toughness of the three-dimensional graphene foam, and makes the composite scaffold It has good electrical conductivity, and when it is applied to cell culture, it can make cells adhere and spread, and can regulate cell behavior through chemically bonded biological factors.

The invention discloses a concentration gradient generation device for gel 3D cell culture, which includes a multi-stage mixing and concentration gradient generation module, and a gel chamber with a multi-stage concentration gradient range, which can simultaneously load multiple gel samples , and through the application of segmental concentration gradients, the detection and evaluation of cell behavior in gels in different concentration gradient ranges is carried out at the same time; the establishment of multi-level concentration gradient ranges can simultaneously detect cells in different concentrations on the same fluid chip. span samples; the fluid chip provided by the present invention has the advantages of small size, low cost, and good integration performance with microgels, and has broad applications in in vitro tissue reconstruction, cell physiology, pathology, and drug screening, biomedicine, and environmental monitoring. Application prospect; at the same time, the invention also discloses its preparation method and application method.