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Anisotropic micro-trend structure material, preparation method thereof and application of material

An anisotropic and structural material technology, applied in metal material coating process, ion implantation plating, coating, etc., can solve the problems of limited range of microstructure, limited range of gradient change, and practical application limitations, etc., to achieve The effect of good practical application potential

Inactive Publication Date: 2018-12-25
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, the design of the cell migration surface was usually to construct a gradient microstructure array, or a gradient soft and hard substrate. The power of the cell migration behavior caused by this microstructure mainly comes from the gradient change of the surface. The greater the change, The more obvious the force is, and the range of this gradient change is limited, so the range that the microstructure can be constructed is also limited, which greatly limits its practical application.

Method used

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  • Anisotropic micro-trend structure material, preparation method thereof and application of material
  • Anisotropic micro-trend structure material, preparation method thereof and application of material
  • Anisotropic micro-trend structure material, preparation method thereof and application of material

Examples

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Effect test

Embodiment 1

[0066] A kind of anisotropic microtendency structure material, its preparation method is as follows figure 1 shown, including:

[0067] 1. The silicon substrate template containing the microporous structure (the size of the holes on the silicon substrate is 5 microns in depth, 5 microns in diameter, 5 microns in spacing between the holes, and a construction range of 2 cm × 2 cm), using absolute ethanol, Ultrasonic cleaning with acetone twice under low power conditions, each time for 5 minutes, after cleaning, use nitrogen to dry the surface;

[0068] 2. Put the dried surface into an airtight container, drop a few drops of dimethyldichlorosilane into the container to fluorinate the surface to reduce the surface activation energy, and the fluorination time is 30 minutes;

[0069] 3. During the fluorination process of the substrate, mix the PDMS prepolymer and the curing agent according to the mass ratio of 10:1, stir the mixed solution into a vacuum desiccator, pump for 1 hour,...

experiment example 1

[0080] Utilize the anisotropic microtaxis structure material that embodiment 1 makes to study the migration of cell on its surface:

[0081] 1. Transfer the modified anisotropic material to ultraviolet light for 2 hours of irradiation and sterilization, and use it as a substrate for cell migration experiments:

[0082] 2. Use trypsin to digest melanoma cells overgrown in a culture dish and prepare a cell suspension of 10,000 cells per milliliter;

[0083] 3. Put the modified PDMS sample into a small petri dish (diameter 35mm, height 10mm), add 3mL of the above cell suspension to the petri dish and transfer to the living cell workstation;

[0084] 4. Connect the living cell workstation to an inverted fluorescence microscope, set it at 37°C, and inject 5% CO2 gas for 24 hours of living cell culture;

[0085] 5. During the culture process, use the camera system attached to the microscope to take pictures of the observation area, and evaluate the migration of cells on the surface...

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Abstract

The invention relates to an anisotropic micro-trend structure material, a preparation method thereof and an application of the material. The anisotropic micro-trend structure material comprises a substrate, at least one surface of the substrate is provided with a projecting micro-structure, a cell adhesion resistant interface layer is further arranged on the surface of the substrate, and a chromium layer, a gold nanoparticle layer and a cell adhesion promoting layer are sequentially arranged on the same side of the cell adhesion resistant interface layer of each projecting micro-structure. Cell adhesion resistant PEG (polyethylene glycol) molecular chains are modified on a PDMS (polydimethylsiloxane) surface to obtain a cell adhesion resistant interface, the gold nanoparticle layer is deposited on one side of a micro-column in an inclined evaporation mode, and RGD (arginine-glycine-aspartic acid) polypeptides promoting cell adhesion are modified to a gold-plated surface to finally obtain an anisotropic cell adhesion micro-trend structure. The structure has the functions of inducing and changing cell migration and has excellent research significance and application values for drug-stimulation-free cell behaviors, research for migration behavior mechanisms of various cells and regulation of diffusion of tumor cells in an organism.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and in particular relates to an anisotropic microtaxis structure material and its preparation method and application. Background technique [0002] Many studies have shown that when cells are in contact with the surface of materials with micro-nano structures, many strange biological behaviors will occur, mainly because the proteins on the surface of the cell membrane will respond after the micro-nano structures contact with cells, resulting in extreme An important factor in cell migration is the polarization of the cytoskeleton. Constructing a controllable cell migration interface on the material surface is of great significance for the study of cell behavior and the transplantation of biomaterials. [0003] Anisotropic microstructures have been widely studied in the field of micro-nano structures in recent years. Due to their special asymmetry, they have good applications in the fields of...

Claims

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Application Information

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IPC IPC(8): C08J7/12C23C14/20C23C14/35C08L83/04
CPCC08J7/12C08J2383/04C08J2471/02C08J2489/00C23C14/205C23C14/35
Inventor 聂壹峰刘思迪李想韩东
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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