299 results about "Adhesion process" patented technology

The invention discloses recombinant human XVII type collagen as well as a preparation method and application thereof. The recombinant human XVII type collagen is obtained by repeating an amino acid sequence from natural human XVII type collagen. The amino acid sequence of the recombinant human XVII type collagen is derived from natural human XVII type collagen, and the recombinant human XVII type collagen does not generate adverse immune response when being used for a human body. The recombinant human XVII type collagen can be secreted and expressed in a commercial yeast expression system, the expression quantity is greater than 10g / L, the preparation method is simple, and the high-yield recombinant human XVII type collagen can be obtained at low cost; in addition, the cell adhesion promoting activity of the recombinant human XVII type collagen is obviously superior to that of human collagen.

The invention provides a catechol functionalized gelatin microsphere and application of the catechol functionalized gelatin microsphere in preparation of a product for inhibiting formation of subcutaneous effusion and promoting tissue repair, and relates to the technical field of biological materials.According to the designed and prepared catechol functionalized gelatin microsphere, catechol groups are introduced into the gelatin microsphere, and the catechol functionalized gelatin microsphere is prepared; good liquid absorption performance and biodegradability are realized. Cell experiment results show that the catechol functionalized gelatin microspheres have super multiple interfaces and can promote cell adhesion and proliferation, and the loose structure of the catechol functionalized gelatin microspheres can promote cell migration. In-vivo rabbit chest wall defect repair experiment results show that the catechol functionalized gelatin microspheres not only can inhibit formation of subcutaneous effusion, but also can collect autologous cells in situ and promote proliferation of the autologous cells and secretion of extracellular matrixes (such as collagen). The results show that the catechol functionalized gelatin microsphere material has clinical application potentials of inhibiting formation of subcutaneous effusion and promoting healing of subcutaneous wounds.

Provided is a method for biosynthesis of a human structural material type IV collagen. Provided herein is a recombinant collagen, comprising sequences as shown in SEQ ID NO: 1 and the like. The recombinant collagen herein has good cell adhesion activity. The method herein relates to using the genetic engineering technology to produce the recombinant collagen, and overcomes defects in the prior art.

The invention discloses a collagen-based composite material and a preparation method thereof. The invention belongs to the technical field of composite materials. The collagen-based composite material comprises the following components in parts by weight: 50-60 parts of collagen; 10 to 20 parts of nano cellulose; 20 to 30 parts of a reinforcing component; 1-3 parts of a cross-linking agent; and 0.5-2 parts of a functional additive. By adding the reinforcing component, the mechanical strength of the collagen-based composite material is remarkably improved, and meanwhile, the non-toxic cross-linking agent and the natural reinforcing component are adopted, so that the material has good biocompatibility and does not generate toxic or adverse reaction on cells and tissues; according to the present invention, the porous three-dimensional network structure is provided, the pore size is 50-300 [mu] m, the porosity is more than or equal to 80%, the cell adhesion, the proliferation and the nutrient substance transmission are easily achieved, and the method is suitable for tissue engineering and wound repair; according to the collagen-based composite material disclosed by the invention, through reasonable raw material formula design, the collagen, the nano cellulose and the natural polysaccharide are compounded, the advantages of all the components are fully exerted, and the comprehensive performance of the composite material is further improved.

The invention provides a methacrylic anhydride modified sclera extracellular matrix repair material and a curing method thereof, and belongs to the technical field of medical repair materials. The methacrylic anhydride modified sclera extracellular matrix repair material provided by the invention is prepared from the following raw materials: a methacrylic anhydride modified sclera extracellular matrix, methacrylated gelatin, a photoinitiator and a solvent. The sclera extracellular matrix modified by methacrylic anhydride is adopted, methacrylic anhydride contains methacrylic acid, double bonds can be introduced, the sclera extracellular matrix has the photo-initiation polymerization capacity, and in-situ gelling can be carried out at sclera and cornea damage positions under the action of a photoinitiator; the sclera extracellular matrix has good biocompatibility and histocompatibility, so that sclera matrix cells and corneal epithelial cells are adhered and proliferated, and the sutureless repair is realized; the methacrylated gelatin can provide a photo-crosslinking gelatin network under the action of a photoinitiator, so that cell adhesion and proliferation can be promoted, and repair is realized.

The invention relates to the technical field of medical instruments, in particular to a multi-layer ultra-light hernia patch which comprises a large-mesh electrostatic spinning tissue ingrowth layer, a mesh woven base material layer and an anti-adhesion layer which are sequentially connected in a stacked mode. Wherein after the large-mesh electrostatic spinning tissue ingrowth layer is implanted into a human body, a cell microenvironment can be better simulated, cell adhesion, differentiation and migration are facilitated, uniform distribution of newborn cells in the fiber direction of an electrospinning membrane is promoted, granulation tissues uniformly ingrow in a defect part, and the electrospinning fiber membrane and the tissues are cooperatively repaired; secondly, the middle layer can provide supporting performance and mechanical performance of the patch in the early stage for the patch, the defects of the electrospinning fiber membrane in the supporting performance and the mechanical performance are overcome, the long-term implantation amount of the patch after later degradation is greatly reduced, and the foreign body sensation is obviously reduced; the anti-adhesion layer also adopts the electrostatic spinning technology, and the anti-adhesion effect is achieved by selecting materials and regulating and controlling the porosity, the aperture and the fiber diameter of the electrospun fiber membrane.

The invention discloses an intelligent integrated system for manufacturing a biological tissue engineering scaffold, relates to the technical field of biomedicine, and is used for improving the biocompatibility, mechanical strength and cell adhesion capacity of a biological scaffold. The plasma generator comprises a microwave generator, a precise electrode and an efficient gas flowing unit; the integrated manufacturing module comprises a 3D printing unit, a plasma enhanced chemical vapor deposition (PECVD) unit and a cooperative control unit; the monitoring and control system comprises an intelligent temperature and humidity sensor, a gas component monitor and an automatic control unit; the data management and optimization module comprises a biological safety evaluation unit, a personalized design and simulation unit, a material selection and management unit, a degradable material processing unit and a dynamic feedback and adjustment unit. According to the invention, low-temperature plasma treatment, 3D printing and PECVD technologies are fused, and a highly intelligent and integrated manufacturing platform is constructed.

The present invention belongs to the technical field of optimized coding gene, and discloses a recombinant human type I collagen, a preparation method thereof and an application thereof, and its amino acid sequence is shown as SEQ ID No.1. The recombinant type I collagen provided by the present invention has a high expression level, has better cell migration promotion, cell proliferation promotion and cell adhesion promotion properties, has high safety, and can be widely applied to fields such as medicine, medical devices, biological materials, tissue engineering, cosmetics and the like.

Provided is a method for biosynthesis of a human structural material type XVII collagen. The collagen comprises the amino acid sequence shown in SEQ ID NO: 2 or a variant amino acid sequence after mutation of the amino acid sequence. The variant amino acid sequence retains the function of the amino acid sequence as shown in SEQ ID NO: 2. The collagen can promote cell adhesion and has a triple helix structure.

The invention discloses a biconvex crystalline lens organ relying on stiffness-controllable hydrogel external directional induction as well as a construction method and application thereof. A crystalline lens organ model provided by the invention is jointly realized by virtue of stiffness-controllable hydrogel and a composite growth factor; and the pluripotent stem cells are rapidly and directionally induced into crystalline lens organs with high quality in vitro. According to the technical scheme, the method comprises the following steps: (1) directionally inducing and differentiating the pluripotent stem cells into primary ectoderm cell clusters; (2) preparing hydrogel with specific rigidity and carrying out surface cell adhesion treatment; (3) planting the primary ectoderm cells on the cleaned polyacrylamide hydrogel and inducing the primary ectoderm cells into a neural ectoderm cell cluster; and (4) inducing the neural ectoderm cells to directionally differentiate into mature crystalline lens organs. The three-dimensional lens organ-like model obtained by the method has a sphere-like biconvex morphology with a top surface and a bottom surface, is transparent and has structures such as capsule membranes, lens epithelial cells and lens fibrocytes, and the bottom surface is also wrapped by a complete lens capsule membrane. Compared with the prior art, the method realizes the formation of a complete capsule film on the bottom surface and a sphere-like form. The obtained crystalline lens organ can be used for research on crystalline lens embryonic development, research on cataract pathogenesis and research, development and screening of cataract drugs.

The invention discloses an active bone defect repair material and a preparation method thereof. The bone defect repair material is prepared by compounding collagen extracted from an animal source, a calcined bone matrix and bone morphogenetic protein composite microspheres and molding through a photocuring 3D printing technology. From the perspective of bionics, collagen is used as a framework material, a calcined bone matrix with low immunogenicity is used as a hard material to enhance mechanical support of the material, and bone morphogenetic protein composite microspheres have a synergistic effect, so that undifferentiated mesenchymal stem cells are guided to develop towards a specific direction in a long-acting manner. After the active bone defect repair material is implanted into a bone defect part, a microenvironment beneficial to cell adhesion and proliferation is provided for cells, and meanwhile, bone morphogenetic protein is slowly released and can act on the bone defect part for a long time, so that the differentiation and maturation process of osteoblasts is effectively promoted, and bone repair of the bone defect part is promoted.

A collagen membrane with an effect of inducing ordered remodeling of tissues, a preparation method therefor, and use thereof, relating to the technical field of surgical supply materials. The preparation method for the collagen membrane comprises the following steps: subjecting a fiber membrane to a heat pressing treatment to obtain the collagen membrane with the effect of inducing ordered remodeling of tissues. The fiber membrane is formed by cross-stacked fibers with a diameter of 0.5-15 μm. The fibers comprise collagen and poly(L-lactide-co-caprolactone). The mass ratio of the collagen to the poly(L-lactide-co-caprolactone) is 1:(1.5-2.5). By combining the collagen, which has good biocompatibility and an effect of promoting tissue repair, with PLCL, which is biodegradable and features excellent mechanical properties and good cell adhesion, the collagen membrane not only has an extracellular matrix-like structure, but also exhibits good structural stability, facilitating the regulation of cell behaviors and tissue regeneration, and effectively promoting the growth, adhesion, and proliferation of host cells, thereby inducing ordered remodeling of tissues.

The invention provides a preparation method of a double-layer guided tissue regeneration membrane, the double-layer guided tissue regeneration membrane and application of the double-layer guided tissue regeneration membrane, and relates to the field of biomedical materials. The preparation method comprises the following steps: carrying out enzymolysis treatment on pretreated fish skin by combining an acid method with a restrictive enzymolysis method to obtain telopeptide-removed collagen; carrying out electrostatic spinning on the blended spinning solution of the telopeptide-removed collagen and the polylactic acid to form a loose layer, and carrying out electrostatic spinning on the loose layer by adopting the polylactic acid spinning solution to form a compact layer, so as to obtain a double-layer composite membrane; and crosslinking the double-layer composite membrane to obtain the double-layer guided tissue regeneration membrane. The double-layer guided tissue regeneration membrane has low immunogenicity, good mechanical properties and excellent histocompatibility, the compact layer can play a barrier role and prevent fibroblasts from occupying an osteogenic space, the loose surface can promote cell adhesion and growth and accelerate bone tissue repair, and the interface of the double-layer structure is tightly combined.

The invention belongs to the technical field of orthopedic implants, and particularly relates to a porous bone grafting-free window type interbody fusion cage and a preparation method thereof. The multi-hole type bone grafting window-free interbody fusion cage comprises a frame body which comprises four side faces and two end faces, and the four side faces are of hollow structures; the space defined by the frame body is filled with the porous structure body; the meshing teeth are arranged on the upper surface and the lower surface of the frame body; and the clamping structure is arranged at one end part of the frame body. According to the porous bone grafting-free window type interbody fusion cage, due to the design of the bone grafting-free window, the complexity of the use process is reduced, the operation is convenient, and the possibility that the fusion cage is polluted is reduced; the porous structure body with the bionic bone trabecula can be printed through 3D printing, and pore channels for bone tissue to grow in and a surface beneficial to cell adhesion can be effectively provided; the hydroxyapatite coating improves the biological activity of the surface of the base material and enhances the interface integration of the interbody fusion cage and the upper and lower end plates of the vertebral body.

The invention relates to the technical field of biomedicine, in particular to hyaluronic acid hydrogel for simulating magnetic regulation and control of cell adhesion performance of tumor ECM (extracellular matrix). The hydrogel is prepared from the following components in percentage by weight: 0.5 to 10 percent of thiolated polysaccharide, 0.01 to 2.5 percent of 2-hydroxy-4 '-(2-hydroxyethoxy)-2-methyl propiophenone Irgacure2959, 0 to 100 [mu] g / mL of a superparamagnetic iron oxide nanorod SPIONR, 0 to 100 [mu] g / mL of a cell adhesion ligand and the balance of a solvent. According to the hyaluronic acid hydrogel capable of simulating the magnetic regulation and control of the cell adhesion performance of the tumor ECM, provided by the invention, the isotropic or anisotropic presentation of a cell adhesion ligand is regulated and controlled by magnetic arrangement through the magnetic nanorod coating soft hydrogel with a high aspect ratio; the hydrogel combines RGD modified superparamagnetic nanoparticles with a hyaluronic acid matrix, the arrangement of the nanoparticles is accurately regulated and controlled through an external magnetic field, and dynamic simulation of an ECM anisotropic topological structure is realized.

The invention belongs to the technical field of semiconductors, and particularly relates to a simultaneous multi-wafer pre-bonding device which comprises a rack, a membrane material supply assembly, a membrane material separation assembly, a membrane material recovery assembly, a membrane material feeding assembly, a membrane material separation assembly, a membrane material recovery assembly, a membrane material feeding assembly, a membrane material separation assembly, a membrane material recovery assembly and a membrane material pre-bonding assembly, and the membrane material feeding assembly, the membrane material separation assembly and the membrane material recovery assembly are arranged on the upper surface of the rack. A vacuum pressing cavity assembly is arranged on the upper surface of the rack, and a jacking mechanism is arranged on the upper surface of the rack. The second Z-axis driving unit is beneficial for driving the upper cavity to move downwards to be combined with the lower cavity to form a closed cavity, so that the closed cavity can be vacuumized by a molecular pump, micro bubbles cannot be generated in the bonding process of a wafer and a lower substrate in the pre-packaging bonding process, the yield can be improved, and the production cost can be reduced. Through the arrangement of the five-axis pressure applying mechanism, the air bag pressing mechanism and the jig heating and cooling unit above the air bag pressing mechanism can be conveniently pushed to move downwards, in addition, CDA gas can be inflated into an air bag in the air bag pressing mechanism through a proportional valve, and therefore an upper pressing plate is made to press a wafer.

The invention discloses a shape memory alloy bone scaffold imitating a Haversian system as well as a 4D printing preparation method and application of the shape memory alloy bone scaffold. According to the 4D printing preparation method, the stress state and function requirements of the bone scaffold are fully considered, a bone scaffold porous structure with an imitated Haversian system is designed by adopting a function bionic method, and the bone scaffold is prepared by adopting a 4D printing technology. The inner layer imitated cancellous bone porous structure designed in the preparation method not only meets the requirement that the inner layer has good elasticity and toughness, but also facilitates cell adhesion to guide cell growth and maintains blood supply; the designed artificial cortical bone porous structure with the Haversian system on the outer layer meets the requirement that the outer layer has good compression resistance and bending resistance, and the Haversian system is more beneficial to ingrowth of blood vessels and nerves and maintains skeleton metabolism and repair. The shape memory alloy bone scaffold simulating the Haversian system, disclosed by the invention, has a wide application prospect in the field of bone implants.

The invention discloses a collaborative cleaning method and an adhesion device for a camera module image sensor, and provides a collaborative cleaning scheme of first wiping and then adhesion in order to solve the technical problems of tool chippings and cleaning liquid residues caused by automatic wiping in the prior art. The method comprises the following steps: firstly wiping and cleaning the sensing surface of the image sensor (S1), then removing wiping residues by using an adhesion device (S2), and finally judging the cleaning effect through visual inspection (S3). A matched adhesion device comprises a cleaning adhesive tape winding and unwinding system, a soft pressing head and a control system, and efficient removal of wiping residues is achieved by accurately controlling adhesive tape adhesion (the adhesive force is 1-5 N / 25 mm) and stepping replacement (the replacement length each time is larger than or equal to the long edge of the rectangle of the sensing face of the image sensor). According to the method, the technical prejudice of the industry is broken through, the traditional wiping and adhesion processes which are considered to repel each other are organically combined, the cleaning yield of automatic equipment of the camera module is increased to 99% or above, and the key technical problem that residues are generated during wiping is solved.

The invention relates to the technical field of biomedical materials, and discloses an active composite membrane with an asymmetric double-layer structure as well as a preparation method and application of the active composite membrane. The active composite membrane with the asymmetric double-layer structure is prepared from the following preparation raw materials in percentage by mass: 95 to 98 percent of chitosan; 0.5%-2% of a plant polyphenol cross-linking agent; and 1%-4% of magnesium-based degradable inorganic filler. The active composite membrane has an integrated double-layer structure without interface bonding, the compact layer can effectively block soft tissues, and the porous layer is beneficial to cell adhesion and nutrition delivery; in a wet state, the material is excellent in mechanical property, controllable in degradation and high in cell safety, can effectively inhibit bacteria and promote osteoblast proliferation and differentiation, and can meet the performance requirements of bone defect repair on the material; the preparation method adopts the steps of solvent volatilization, freeze drying and precooled alkaline alcohol solution washing for preparation, the whole preparation process is carried out in a water phase, the conditions are mild, the steps are simple, complex equipment is not needed, and extremely high clinical transformation and industrialization potential is shown.

The invention relates to a flexible glue material with PCO resistance and application thereof, and provides a flexible glue type IOL material which can be injected, adapts to minimally invasive surgery and has infinite zooming capability and biological resistance, a precursor of the material can be injected into an intraocular capsular bag through an injector, an implanted wound is extremely small, the precursor is crosslinked into flexible glue in situ in the intraocular capsular bag, and the flexible glue can be used as an intraocular material. The performance of natural crystals of young people is simulated, so that the cataract patient recovers the natural regulation function after the operation. The implanted flexible glue has the characteristics of cell adhesion resistance, protein deposition resistance and the like, PCO is avoided, compared with an existing IOL product, the service life is longer, and meanwhile better visual quality is provided for a patient.

The present invention relates to an adhesion device (1) for culturing microalgae that increases the density of microalgae cultures and their biomass productivity. The device comprises a body (2) with an upper opening that contains a culture medium (3), which occupies half of its volume; a plurality of rotary discs (4) as a supporting surface on which the microalgae cells (5) adhere, promoting their growth to form a biofilm, said plurality of discs (4) being fixed to a horizontal rotation shaft (6), which passes through the body (2), and being equally spaced apart, the location of the rotation shaft (6) inside the body (2) being such that said discs remain semi-submerged in the culture medium (3); gas-liquid diffusers for gas transfer and bubbling in the culture medium (3); at least one moisture sensor and at least one temperature sensor for monitoring the moisture and temperature, respectively, of the gas phase inside the body (2) of the device (1); at least one temperature sensor for monitoring the temperature of the culture medium (3); an upper cover (7) on the upper opening of the body (2), which prevents the culture medium (3) and microalgae cells (5) from being exposed to the outside environment; a lighting board (8) arranged between each pair of discs (4) of the plurality of discs (4), being located on the rotation shaft (6) and arranged in slots (71) in the cover (7) so that said lighting boards (8) are not in contact with the inside of the body (2); and an electric motor to provide slow and accurate rotation of the rotation shaft (6), wherein the body (2) and the cover (7) are made of a transparent or translucent material that allows sunlight or artificial light to pass through.

The invention discloses an organic-inorganic multi-layer composite coating material with functions of promoting cell adhesion, resisting infection and promoting osteogenesis and a preparation method of the organic-inorganic multi-layer composite coating material. The method specifically comprises the following steps: 1) carrying out primary sand blasting treatment on a titanium / titanium alloy sample, then removing an oxide layer on the surface of the titanium / titanium alloy sample by using an ultrasonic oil removal and glow discharge cleaning process, and constructing a copper / zinc alloy coating on the surface of the sample by combining an electrochemical deposition process; 2) carrying out micro-nano processing on the coating by using a secondary sand blasting process to introduce a topological morphology; (3) depositing a polydopamine PDA coating on the surface by using an in-situ auto-polymerization process, and simultaneously dipping to complete the chelation of Fe < 3 + > and PDA; 4) further utilizing an ultrasonic impregnation process to realize conversion from PDA-Fe < 3 + > chelation to PDA-Fe < 3 + >-salvianolic acid b chelation so as to introduce a salvianolic acid b layer, and washing, air-drying and sterilizing to obtain the material. The material has multiple cell adhesion promotion, infection resistance and multiple osteogenesis promotion functions, and can realize innovation of coating technologies in the fields of orthopedics, dentistry and the like.

Provided is a biosynthesis-based preparation method for a structural material of the human body. A polypeptide of the present application contains the structure of a C-terminal region of (repeating unit)n or (repeating unit)n-; the repeating unit contains an amino acid sequence as shown in SEQ ID NO. 1. Recombinant type V humanized collagen prepared in the present application has high activity in promoting cell adhesion, and does not cause an immune response when applied to the human body; moreover, the preparation method is novel, allows for large-scale production of the recombinant type V humanized collagen, and is widely used in the preparation of structural materials of the human body. The application field includes preparation of high-end medical devices, such as biological dressings, human body biomimetic materials, plastic-beauty materials, organoid culture, cardiovascular stents, coatings, tissue injection filling, ophthalmic materials, gynecological biomaterials, nerve repair and regeneration, liver tissue and vessel repair and regeneration, and 3D printing of artificial organ biomaterials.

The invention relates to the technical field of preparation of polycaprolactone porous membranes, in particular to a polycaprolactone porous membrane and a preparation method and application thereof.The polycaprolactone porous membrane is of a porous structure with macropores and small pores coexisting, the pore walls of the macropores and the small pores are all composed of polycaprolactone particles, and the polycaprolactone porous membrane is provided with pores with the pore walls composed of micron-sized particles. The polycaprolactone porous membrane is prepared from polycaprolactone, a composite hierarchical pore structure containing small pores in macropores is formed, a three-dimensional pore network internally communicated with one another is formed, the specific surface area is increased by the porous structure, the number of adhered cells is further increased, the interconnected porous structure allows cells to migrate into the polycaprolactone porous membrane, and meanwhile, the porous structure is convenient for conveying substances.

According to the preparation method of the moisturizing wound protecting membrane, the wound protecting membrane is of a double-layer structure and comprises a polyurethane outer membrane and a composite membrane composed of collagen sponge and an animal intestine membrane, and the preparation method of the moisturizing wound protecting membrane comprises the following steps that S1, the polyurethane outer membrane is prepared; s2, preparing a film layer; s3, preparing a collagen layer; s4, freeze-drying and molding the collagen layer and the membrane layer in a mold to obtain a collagen membrane; and S5, bonding the obtained polyurethane outer membrane with a collagen membrane to prepare the double-layer wound protecting membrane. The wound protecting membrane provided by the invention adopts a design of tightly combining three layers, the acellular matrix middle layer can be gradually degraded to inhibit scar hyperplasia, the polyurethane outer membrane provides a physical barrier and promotes cell adhesion and wound healing, and the three-dimensional porous collagen sponge bracket accelerates vascularization and cell ingrowth, so that the wound protecting membrane has a good wound healing effect. A novel decellularization technology is integrally adopted to retain biological activity, and an environment beneficial to skin growth is constructed through a double-layer structure.

This invention discloses an interlocking bone tissue repair scaffold, assembled from several notched links connected in an interlocking manner. The notches in one link allow passage through the notches in another link, but not through the remaining main body of the other link. The links are 3D printed from calcium phosphate ceramic powder. The porosity of the final assembled chain can be flexibly controlled by adjusting the shape, size, thickness, and combination of links of different sizes, allowing for precise customization according to specific application scenarios. This scaffold structure is flexible and adjustable, adaptable to different implantation areas, significantly expanding the product's applicability. After implantation, it creates a microenvironment more closely aligned with human tissue growth patterns, optimizing local tissue compatibility and providing excellent conditions for cell adhesion, retention, and proliferation, effectively improving the scaffold's biocompatibility and clinical application efficacy.

The lightweight melt-blown hot-melt nonwoven fabric containing hydrophobic nanosilica according to the manufacturing method of the present invention performs an adhesive function when interposed between adherends such as fabric, even if a smaller amount of adhesive resin is applied than a conventional hot-melt film, it has the effect of saving material costs and energy by about 10 to 50% while ensuring uniformity of adhesive strength for each section, excellent peel strength, and good breathability. As a result, fabric products using the melt-blown hot-melt nonwoven fabric can prevent overflow during the adhesion process and achieve lightness and a soft texture.

The present invention relates to a cell culture substrate comprising a substrate and a layer containing a hydrophilic polymer that covers at least part of the surface of the substrate, where the cell culture substrate has an (A) region and a (B) region, the (A) region has cell adhesion and cell proliferation properties, and the (B) region is adjacent to the (A) region and does not have cell adhesion or cell proliferation properties, wherein: the (A) region comprises a recess formed in the layer containing the hydrophilic polymer; the recess has an inclined surface and a bottom surface; and the angle of inclination of the inclined surface is 40° to 110°.

The utility model discloses a differential adherent cell separation device which comprises a separation box body and an upper box cover, culture cavities and a one-way conducting assembly are arranged in the separation box body, the one-way conducting assembly is arranged between the culture cavities, the side face of the separation box body extends outwards to form a supporting part, the side edge of the supporting part protrudes upwards to form a baffle ring part, and the baffle ring part is connected with the upper box cover. A limiting slot is formed among the baffle ring part, the supporting part and the separating box body, and the four sides of the upper box cover extend downwards to form side ring parts. According to the utility model, the one-way conduction between the culture cavities is realized through the one-way conduction assembly, the communication state between the culture cavities can be controlled at a proper stage according to the actual condition of cell wall attachment, the cells are ensured to flow according to the expected direction and mode, and the accuracy and purity of cell separation are effectively improved; the upper box cover and the separation box body are tightly and stably connected, the upper box cover is prevented from loosening or displacing in the using process, and the overall sealing performance and stability of the device are guaranteed.

A biomimetic intervertebral fusion device with a piezoelectric multifunctional coating belongs to the field of implantable medical devices. It includes an upper piezoelectric multifunctional coating, a fusion device body, and a lower piezoelectric multifunctional coating. A bone graft window is located in the middle of the biomimetic intervertebral fusion device. The piezoelectric multifunctional coating is made of electrospun piezoelectric PVDF / HA fibers, which are conformally adhered to the upper and lower surfaces of the fusion device body through a polydopamine adhesive layer. The upper and lower surfaces of the fusion device body have a biomimetic curved surface configuration to match the morphology of the vertebral endplates, increasing the contact area and reducing stress concentration at the fusion interface. While maintaining an elastic modulus similar to natural bone and good radiation permeability, this fusion device, after implantation, can create a microenvironment conducive to cell adhesion and proliferation. Under the influence of cell traction, ultrasonic stimulation, and the physiological load of the human lumbar spine, it generates electrical stimulation signals, promoting bone fusion, improving the stability of the fusion interface, and reducing the risk of postoperative loosening and subsidence of the fusion device.