97 results about "Adhesion process" patented technology

The invention relates to the technical field of biomedical materials, and discloses an active composite membrane with an asymmetric double-layer structure as well as a preparation method and application of the active composite membrane. The active composite membrane with the asymmetric double-layer structure is prepared from the following preparation raw materials in percentage by mass: 95 to 98 percent of chitosan; 0.5%-2% of a plant polyphenol cross-linking agent; and 1%-4% of magnesium-based degradable inorganic filler. The active composite membrane has an integrated double-layer structure without interface bonding, the compact layer can effectively block soft tissues, and the porous layer is beneficial to cell adhesion and nutrition delivery; in a wet state, the material is excellent in mechanical property, controllable in degradation and high in cell safety, can effectively inhibit bacteria and promote osteoblast proliferation and differentiation, and can meet the performance requirements of bone defect repair on the material; the preparation method adopts the steps of solvent volatilization, freeze drying and precooled alkaline alcohol solution washing for preparation, the whole preparation process is carried out in a water phase, the conditions are mild, the steps are simple, complex equipment is not needed, and extremely high clinical transformation and industrialization potential is shown.

The invention discloses an organic-inorganic multi-layer composite coating material with functions of promoting cell adhesion, resisting infection and promoting osteogenesis and a preparation method of the organic-inorganic multi-layer composite coating material. The method specifically comprises the following steps: 1) carrying out primary sand blasting treatment on a titanium / titanium alloy sample, then removing an oxide layer on the surface of the titanium / titanium alloy sample by using an ultrasonic oil removal and glow discharge cleaning process, and constructing a copper / zinc alloy coating on the surface of the sample by combining an electrochemical deposition process; 2) carrying out micro-nano processing on the coating by using a secondary sand blasting process to introduce a topological morphology; (3) depositing a polydopamine PDA coating on the surface by using an in-situ auto-polymerization process, and simultaneously dipping to complete the chelation of Fe < 3 + > and PDA; 4) further utilizing an ultrasonic impregnation process to realize conversion from PDA-Fe < 3 + > chelation to PDA-Fe < 3 + >-salvianolic acid b chelation so as to introduce a salvianolic acid b layer, and washing, air-drying and sterilizing to obtain the material. The material has multiple cell adhesion promotion, infection resistance and multiple osteogenesis promotion functions, and can realize innovation of coating technologies in the fields of orthopedics, dentistry and the like.

The invention relates to the technical field of preparation of polycaprolactone porous membranes, in particular to a polycaprolactone porous membrane and a preparation method and application thereof.The polycaprolactone porous membrane is of a porous structure with macropores and small pores coexisting, the pore walls of the macropores and the small pores are all composed of polycaprolactone particles, and the polycaprolactone porous membrane is provided with pores with the pore walls composed of micron-sized particles. The polycaprolactone porous membrane is prepared from polycaprolactone, a composite hierarchical pore structure containing small pores in macropores is formed, a three-dimensional pore network internally communicated with one another is formed, the specific surface area is increased by the porous structure, the number of adhered cells is further increased, the interconnected porous structure allows cells to migrate into the polycaprolactone porous membrane, and meanwhile, the porous structure is convenient for conveying substances.

This invention discloses an interlocking bone tissue repair scaffold, assembled from several notched links connected in an interlocking manner. The notches in one link allow passage through the notches in another link, but not through the remaining main body of the other link. The links are 3D printed from calcium phosphate ceramic powder. The porosity of the final assembled chain can be flexibly controlled by adjusting the shape, size, thickness, and combination of links of different sizes, allowing for precise customization according to specific application scenarios. This scaffold structure is flexible and adjustable, adaptable to different implantation areas, significantly expanding the product's applicability. After implantation, it creates a microenvironment more closely aligned with human tissue growth patterns, optimizing local tissue compatibility and providing excellent conditions for cell adhesion, retention, and proliferation, effectively improving the scaffold's biocompatibility and clinical application efficacy.

The lightweight melt-blown hot-melt nonwoven fabric containing hydrophobic nanosilica according to the manufacturing method of the present invention performs an adhesive function when interposed between adherends such as fabric, even if a smaller amount of adhesive resin is applied than a conventional hot-melt film, it has the effect of saving material costs and energy by about 10 to 50% while ensuring uniformity of adhesive strength for each section, excellent peel strength, and good breathability. As a result, fabric products using the melt-blown hot-melt nonwoven fabric can prevent overflow during the adhesion process and achieve lightness and a soft texture.

The utility model discloses a differential adherent cell separation device which comprises a separation box body and an upper box cover, culture cavities and a one-way conducting assembly are arranged in the separation box body, the one-way conducting assembly is arranged between the culture cavities, the side face of the separation box body extends outwards to form a supporting part, the side edge of the supporting part protrudes upwards to form a baffle ring part, and the baffle ring part is connected with the upper box cover. A limiting slot is formed among the baffle ring part, the supporting part and the separating box body, and the four sides of the upper box cover extend downwards to form side ring parts. According to the utility model, the one-way conduction between the culture cavities is realized through the one-way conduction assembly, the communication state between the culture cavities can be controlled at a proper stage according to the actual condition of cell wall attachment, the cells are ensured to flow according to the expected direction and mode, and the accuracy and purity of cell separation are effectively improved; the upper box cover and the separation box body are tightly and stably connected, the upper box cover is prevented from loosening or displacing in the using process, and the overall sealing performance and stability of the device are guaranteed.

A biomimetic intervertebral fusion device with a piezoelectric multifunctional coating belongs to the field of implantable medical devices. It includes an upper piezoelectric multifunctional coating, a fusion device body, and a lower piezoelectric multifunctional coating. A bone graft window is located in the middle of the biomimetic intervertebral fusion device. The piezoelectric multifunctional coating is made of electrospun piezoelectric PVDF / HA fibers, which are conformally adhered to the upper and lower surfaces of the fusion device body through a polydopamine adhesive layer. The upper and lower surfaces of the fusion device body have a biomimetic curved surface configuration to match the morphology of the vertebral endplates, increasing the contact area and reducing stress concentration at the fusion interface. While maintaining an elastic modulus similar to natural bone and good radiation permeability, this fusion device, after implantation, can create a microenvironment conducive to cell adhesion and proliferation. Under the influence of cell traction, ultrasonic stimulation, and the physiological load of the human lumbar spine, it generates electrical stimulation signals, promoting bone fusion, improving the stability of the fusion interface, and reducing the risk of postoperative loosening and subsidence of the fusion device.

The invention relates to a preparation method of an exosome-loaded photocuring hydrogel scaffold capable of promoting bone repair and a product thereof, and relates to the technical field of biological materials and tissue engineering. According to the main thought, exosome is loaded in gelatin hydroxyapatite microspheres, then the microspheres are evenly dispersed in a three-dimensional continuous network formed by light-cured hydrogel, and a microsphere matrix and a light-cured hydrogel material are the same or different. The invention has the advantages that: the photocuring hydrogel provides macroscopic physical support and a cell adhesion skeleton; and the dispersed exosome loaded hydroxyapatite microspheres are used as an independent repository, so that protection and controllable release of active substances are realized, and meanwhile, the mechanical strength of the hydrogel is enhanced. And the structure is accurate and controllable, the composite slurry can be molded into any complex two-dimensional or three-dimensional structures through a mold or a biological 3D printing technology by utilizing the photocuring characteristic, and the personalized repair requirement is met.

The invention discloses a medical-grade PEEK composite material for 3D printing and a preparation method of the medical-grade PEEK composite material, relates to the technical field of 3D printing, and solves the problem of low biological activity of an existing PEEK material. The medical-grade PEEK composite material is prepared from the following raw materials in parts by weight: 80-95 parts of a polyether-ether-ketone matrix, 5-15 parts of a bioactive filler, 0.5-5 parts of an interface modifier and 0.1-3 parts of a heat conduction and nucleation enhancer, the bioactive filler comprises nano-hydroxyapatite and bioactive glass in a weight ratio of (1-5): (5-1). The natural bone environment can be effectively simulated, the biological activity of the composite material is improved, and cell adhesion and proliferation are promoted, so that the cell proliferation rate is increased, and the antibacterial effect and the mechanical property of the composite material can be effectively improved.

The invention belongs to the technical field of biomedical materials, and discloses an anisotropic nanofiber yarn composite scaffold as well as a preparation method and application thereof. The anisotropic nanofiber yarn composite scaffold is formed by compounding methylacryloyl modified gelatin and a nanofiber yarn scaffold prepared through electrostatic spinning. The anisotropic nanofiber yarn composite scaffold not only has more excellent mechanical properties, but also can provide a more suitable microenvironment for cell adhesion and growth, can significantly improve the remodeling efficiency of an extracellular matrix, and has a wide application prospect in the field of heart valve repair.

The utility model belongs to the technical field of orthopedic implants, and particularly relates to a porous bone grafting-free window type interbody fusion cage. The multi-hole type bone grafting window-free interbody fusion cage comprises a frame body which comprises four side faces and two end faces, and the four side faces are of hollow structures; the space defined by the frame body is filled with the porous structure body; the meshing teeth are arranged on the upper surface and the lower surface of the frame body; and the clamping structure is arranged at one end part of the frame body. According to the multi-hole type bone grafting-free window type interbody fusion cage, due to the design of the bone grafting-free window, the complexity of the using process is reduced, the operation is convenient, and the possibility that the fusion cage is polluted is reduced; the porous structure body with the bionic bone trabecula can be printed through 3D printing, and pore channels for bone tissue to grow in and a surface beneficial to cell adhesion can be effectively provided; the hydroxyapatite coating improves the biological activity of the surface of the base material and enhances the interface integration of the interbody fusion cage and the upper and lower end plates of the vertebral body.

The application discloses a high-repetition-frequency laser resonant cavity with a piezoelectric driving function and a pulse locking device. In the resonant cavity, a stacked piezoelectric actuator is subjected to a first adhesion process with a first gasket and a second gasket at both ends, a first organic glass and a second organic glass are subjected to a second adhesion process with the other sides of the first gasket and the second gasket, and a combination based on the stacked piezoelectric actuator is formed; a dielectric film is arranged on the end face of a third plug; a gain optical fiber is inserted and passes through a first plug, the combination based on the stacked piezoelectric actuator and a second plug connected in sequence; the end face of the first plug is butted against a semiconductor saturable absorber mirror, and the end face of the second plug is butted against the dielectric film. The application overcomes the problem of repetition frequency drift in a centimeter-level resonant cavity.

The present invention particularly relates to the field of adhesion of tobacco products. Disclosed are a water-based adhesive for cigarettes, and a preparation method therefor. By adding sodium fluorescein to the adhesive, the adhesive emits bright green fluorescence under ultraviolet light, and the quality detection during production process and anti-counterfeiting function are realized. In addition, sodium bicarbonate and citric acid react to generate carbon dioxide bubbles, thereby enhancing the visualization and controllability of the adhesion process. By means of the improvements, the present invention effectively solves the problems of difficult quality control and poor user experience in the prior art, improves the performance and the application effect of the adhesive, and satisfies the requirements of the modern tobacco industry for efficient, safe and multifunctional adhesives.

The invention discloses a sodium alginate hydrogel medical dressing and a preparation method thereof, and particularly relates to the technical field of medical dressings, the sodium alginate hydrogel medical dressing comprises a material body, a material layer is mounted at the bottom of the material body, and a plurality of material liquid parts are fixed at the bottom of the material layer; the connecting mechanism comprises a connecting layer, a first arc layer and a second arc layer, the connecting layer is fixed to one side of the material body, the first arc layer and the second arc layer are hinged to the two sides of the connecting layer, the first arc layer is matched with the second arc layer, and a soft layer is fixed to one side of the first arc layer; through the arrangement of the connecting mechanism, the wound of a patient can be rapidly covered with the material layer, the operation time in the dressing change process is shortened, the infection risk increased due to the fact that the wound of the patient is exposed on the surface for a long time is avoided, and meanwhile the problem that an existing device is prone to further stimulation on tissue around the wound in the adhesion process is solved; and the pain and discomfort of the patient are relieved, so that the efficiency of the device is greatly improved.

The utility model discloses a vortex oscillation type reactor for preparing mesenchymal stem cell spheres, which comprises a base, a motor and an oscillation support are mounted on the base, a cam is connected onto an output shaft of the motor, the motor drives the cam and the oscillation support to rotate synchronously, and a container bottle fixing support is mounted on the oscillation support through a spring. The container bottle fixing support is used for installing a replaceable container bottle, the motor is provided with a controller, and the controller is used for controlling the rotating speed, the forward and reverse rotation direction and timing start and stop of the motor. According to the utility model, the cells are inhibited from adhering to the wall in a special vortex oscillation mode, the cells are promoted to naturally gather to form spheres, the direct shear damage of mechanical stirring to the cells is reduced, and the cell activity and the sphere forming efficiency are improved; the rotating speed and the direction of the motor can be controlled, the vortex intensity and the oscillation frequency are accurately regulated and controlled, the structure is simple and compact, operation is convenient and fast, and the device is suitable for small laboratories.

This invention relates to a method and system for seamless holographic pattern imprinting on curved substrates, specifically in the field of holographic pattern imprinting on curved surfaces. The method includes: scanning the curved substrate to obtain a curvature curve of the imprinting surface; dividing the curvature curve of the imprinting surface into a curvature range according to a preset allowable curvature fluctuation range; dividing the curved surface into segmented pressure regions and segmented average curvatures based on the curvature range; searching for the corresponding imprinting pressure in a preset curvature pressure database based on the segmented average curvature; controlling corresponding pneumatic adsorption devices to respectively adhere to the areas of the flexible printing plate corresponding to the segmented pressure regions; then controlling a robotic arm to move the flexible printing plate and adhere it to the curved substrate; and applying the corresponding imprinting pressure to the areas of the flexible printing plate corresponding to the segmented pressure regions after the adhesion process. This invention achieves seamless holographic pattern imprinting on curved substrates.

The invention discloses a biological material for vascular intervention and a preparation method thereof, and relates to the technical field of biological materials.The biological material comprises a stent base body and a functional coating coated on the surface of the stent base body, and the stent base body is made of a degradable metal material; the functional coating is composed of a bioactive substance, a drug sustained-release carrier, a nanoparticle enhancer and an intelligent responsive polymer. The biological material and the preparation method have the remarkable advantages that the micro-nano structure of the scaffold matrix promotes cell adhesion and proliferation, and the mechanical property is good; the functional coating realizes controllable release of the medicine and enhances the stability; the preparation process innovation ensures high quality of the material; a sensor and a protective film are integrated, so that the safety and functionality are improved, and the clinical application prospect is wide.

The present application relates to the use of polysialic acid or its derivatives in anti-foreign body reaction, in particular, to a polysialic acid anti-foreign body reaction material for resisting biological contamination, for regulating macrophage phenotype to reduce inflammatory response, and for resisting foreign body reaction. The polysialic acid material includes a bulk material prepared from polysialic acid and its derivatives, or a coating material for surface modification of the material, which can resist protein adsorption, cell adhesion and adhesion of various microorganisms, can act on immune cells, such as regulating the phenotype of macrophages, thereby reducing acute and chronic inflammatory response, and has excellent anti-foreign body reaction effect as an implant material.

The invention provides an enzyme-residue-free extracellular matrix microsphere as well as a preparation method and application thereof. An elutable sacrificial phase material capable of being subjected to liquid-liquid phase separation with an extracellular matrix is introduced in an extracellular matrix pelletizing process, and transglutaminase is confined in a sacrificial phase, so that the extracellular matrix microsphere is prepared. And the transglutaminase and the extracellular matrix form a spatially separated structure in the balling process. In the gel forming stage, transglutaminase catalyzes protein in an extracellular matrix on a two-phase interface to generate a cross-linking reaction, and microspheres with a three-dimensional network gel structure are formed; after cross-linking is completed, transglutaminase is removed by eluting the sacrificial phase material, and meanwhile, a porous network with a communicated structure is formed in situ in the microsphere, so that the obtained extracellular matrix microsphere still keeps good structural integrity and mechanical property while realizing no residue of enzyme with catalytic activity; the scaffold can provide stable support for cell adhesion, migration and three-dimensional culture, and is applicable to cell delivery, organoid construction, tissue engineering scaffolds and the like.

The utility model provides an embryo biopsy needle which comprises a first pipe section and a second pipe section, the first end of the first pipe section is connected with negative pressure equipment, the second end of the first pipe section is connected with the first end of the second pipe section, the second end of the second pipe section is used for sucking biopsy cells, a filter membrane is arranged in the second pipe section, and the filter membrane is connected with the negative pressure equipment. A plurality of micropores are distributed in the filter membrane, liquid and air can pass through the micropores to block biopsy cells, and the surface of the filter membrane is smooth so as to avoid adhesion of the cells. The filter membrane arranged in the second tube section can be used as a blocking net in the biopsy needle, so that biopsy cells cannot be lost due to the fact that the biopsy cells are sucked into the first tube section; meanwhile, the pressure can be better controlled in the biopsy operation process; in addition, sample pollution and the like are avoided.

The application belongs to the technical field of material preparation, and particularly relates to a marine polysaccharide-based bio-ink for in-situ 3D printing and a preparation method and application thereof. The marine polysaccharide-based bio-ink provided by the application forms a hydrogel with good mechanical properties and good biocompatibility, and can be used for in-situ 3D photocuring bio-printing. The hydrogel has the functions of promoting cell adhesion, proliferation, migration, anti-inflammation, anti-oxidation and promoting endothelial cell vascularization, and can be used for in-situ tissue repair, blood vessel construction, tissue reconstruction and the like, and has a high application prospect.

The invention relates to the technical field of culture dishes, in particular to a thermal response polymer graft modified cell lamella transfer culture dish which comprises a culture bin, a plurality of openings are formed in one side of the culture bin, steps corresponding to the openings are arranged in the culture bin, trays are placed at the upper ends of the steps, and convex rings are arranged on the trays. A cavity of a hollow structure is formed in the tray, a grab handle is arranged on one side of the tray, a soaking circulation piece connected with the cavity is arranged on the tray, the soaking circulation piece comprises a connector arranged on one side of the grab handle, a detachable connecting plate is arranged on one side of the connector, and a water inlet part and a water outlet part are arranged on the connecting plate. According to the invention, warm water or low-temperature liquid can be flexibly switched according to culture or transfer requirements, and the temperature of the tray is rapidly adjusted by virtue of a liquid circulation temperature control principle so as to control the hydrophobic and hydrophilic states of the thermal response polymer coating, so that different requirements of cell attachment growth and detachment transfer are met.

The invention discloses application of a Dow Corning SYLGARD 184 organic silicon elastomer in preparation of a three-dimensional cell culture medium, and belongs to the technical field of biological materials. The matrix is formed by mixing an A-agent prepolymer and a B-agent curing agent of SYLGARD 184 according to the weight ratio of (8-12): 1 and then curing at the temperature of 35-39 DEG C. The method is characterized in that the substrate can support cell attachment without plasma treatment, and surface modification can be carried out through I-type collagen to enhance cell adhesion, so that a unique dual-mode cell microenvironment regulation and control system is formed. The matrix provided by the invention is simple to operate, low in cost and high in batch stability, effectively overcomes the defects of uncertain components, complex hydrogel synthesis treatment and the like of the existing animal-derived matrix, and can be widely applied to three-dimensional cell culture, disease model construction and drug screening platforms.

A bioreactor (1) for the culture of cells (C) comprising a stack of carriers (7) for cell (C) adherence and liquid medium (M) distribution. The carriers (7) are stacked so as to define levels (6) between adjacent carriers (7) for the flow of the liquid medium (M). Adjacent levels (6) are fluidly interconnected via open spaces (2) so that the liquid medium (M) can flow from one level (6) to an adjacent level (6). The open spaces (2) between a first and an adjacent second level (6′) do not overlap with the one or more open spaces (2) between the second level (6′) and an adjacent third level (6″). One or more of the carriers may also include an area adapted to prevent cell adhesion or growth, thereby allowing for the viewing of cell growth on adjacent carriers from a vantage point external to the bioreactor. Related methods are also disclosed.

This invention provides a biocompatible β This invention relates to a method for composite modification of titanium alloy surfaces, belonging to the technical field of alloy modification methods. The invention includes applications in medical... β This invention involves introducing bioactive particles onto the surface of a titanium alloy substrate, performing friction stir processing (FSP) on the substrate to introduce bioactive particles and construct a gradient grain structure, and then subjecting the FSP-treated substrate to compressed plasma flow (CPF) treatment to achieve uniform distribution of bioactive particles and construct a topology with an average surface roughness of 3-6 μm suitable for cell growth. The invention precisely controls the amount of bioactive particles introduced and constructs a gradient grain structure through friction stir processing (FSP); simultaneously, compressed plasma flow (CPF) technology achieves uniform distribution of bioactive particles and an ideal surface roughness of 3-6 μm on the titanium alloy surface. Ultimately, this significantly improves the cell adhesion, proliferation capacity, and osteogenic properties of the material surface, solving the technical problem of low integration efficiency of traditional titanium alloy implants with human bone tissue.

The invention discloses functional bioactive glass as well as a preparation method and application thereof, and relates to the technical field of regenerative medical materials.The functional bioactive glass provided by the invention takes silicon, calcium, phosphorus and sodium as basic components, a porous structure is constructed by introducing nano zinc oxide (ZnO), and hydrophilicity and dispersity are improved by utilizing short amylose surface grafting. The nano zinc oxide is used as a pore-forming template and is selectively dissolved out in the subsequent steps to form sphere-like pores with the pore diameter of 1-30nm and the porosity of 30-70%, so that physical support is provided for cell adhesion and bone tissue ingrowth. The short amylose is grafted on the surface of the glass through a covalent bond of an amino silane coupling agent, and hydroxyl (-OH) on a molecular chain of the short amylose and water molecules form a hydrogen bond, so that the contact angle of the material is obviously reduced, and particle aggregation is effectively inhibited.

The application provides a multilayer polymer biomimetic membrane for dura mater repair and a preparation method thereof. The biomimetic membrane comprises a three-layer biomimetic nanofiber composite structure: a cell adhesion inner layer on the side facing the brain tissue, an anti-adhesion outer layer on the side facing the skull, and an intermediate reinforcing layer between the cell adhesion inner layer and the anti-adhesion outer layer. The cell adhesion inner layer is a PCL / chitosan / collagen composite fiber layer, the intermediate reinforcing layer is a PCL / hydroxyapatite composite fiber layer, and the anti-adhesion outer layer is a PCL / polylactic acid-hydroxyacetic acid copolymer (PLGA) layer. The method comprises cell adhesion inner layer preparation, intermediate reinforcing layer preparation, anti-adhesion outer layer preparation, and continuous production processing. The application obtains a multilayer artificial dura mater with strong interlayer bonding, high adhesion, and biomimetic mechanical properties.

The present disclosure is directed to scaffold materials patterned with cell adhesion targets that permit the attachment and growth of predetermined cell types onto the scaffold. By selecting specific ligands that are patterned on the scaffold, particular cell types, including those engineered for this specific purpose, will attach and proliferate to create designed tissues and organs.

In cell culture, the microcarriers are brought into contact with cells adhering to the bottom surface of a first culture substrate having a bottom surface treated for cell adhesion.