Nerve tissue engineering tube type bracket and method for making same

A nerve tissue and engineering technology, applied in the field of biomedical materials and tissue engineering, can solve the problems of unable to maintain the structure, difficult to preset cells, unsuitable for small-bore catheters, etc., to achieve the effect of promoting nerve regeneration

Inactive Publication Date: 2005-03-16
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent application 00810000.4 provides a nerve conduit made of polyester material. The lumen is filled with collagen hydrochloric acid solution and freeze-dried to obtain a collagen content with fine fibrils. However, this method is difficult to obtain a multi-channel structure with axial penetration ; Chinese patent application 02113103.1 puts chitosan fibers into chitosan tubes to prepare pure chitosan nerve guides; Chinese patent application 02105864.4 places folded and curled porosity with concave-convex surfaces in hollow tubes made of polyester materials Polyester films are used to increase the surface area of ​​the cavity to make multi-channel nerve regeneration catheters. However, the contents of the above-mentioned catheters are in a free state, which cannot maintain a stable str

Method used

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  • Nerve tissue engineering tube type bracket and method for making same
  • Nerve tissue engineering tube type bracket and method for making same
  • Nerve tissue engineering tube type bracket and method for making same

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preparation example Construction

[0022] The preparation method provided by the invention is as follows:

[0023] (1) Immerse the polytetrafluoroethylene-coated stainless steel rod mandrel (diameter 1-5mm) in 2-4% (w / v) chitosan solution (prepared with 0.2mol / L acetic acid solution), pull it out, Freeze, then immerse, pull out, and freeze, repeat at least 3 times, then freeze at -20°C to -196°C for 12 to 48 hours, and then freeze and dry in a freeze dryer. Place the dried chitosan tube with mandrel in 2% (w / v) sodium hydroxide solution for 10 to 30 minutes, take it out, rinse it with deionized water to neutrality, and then soak it in 10-30 minutes in phosphate buffer solution with pH=7.2-7.4. Absorb the moisture on the surface of the chitosan tube with filter paper and let it dry naturally. The stainless steel rod mandrel is taken out to finally obtain a semi-permeable chitosan round tube with a wall thickness of 0.4-1.0 mm.

[0024] (2) The preparation of multi-channel matrix can be as attached figure 1 T...

Embodiment 1

[0027] (1) Immerse the stainless steel rod mandrel coated with polytetrafluoroethylene with a diameter of 1mm in 2% (w / v) chitosan solution (prepared with 0.2mol / L acetic acid solution), pull it out, freeze it, and then immerse and pull it out Take out, freeze, repeat 3 times, then freeze at -20 ℃ for 12 hours, and then freeze-dry it in a freeze dryer. The dried chitosan tube with mandrel is placed in 2% (w / v) sodium hydroxide solution and soaked for 10 minutes, taken out, rinsed with deionized water to neutrality, then immersed in pH = 7.2 to 7.4 in phosphate buffered saline for 10 minutes. Absorb the moisture on the surface of the chitosan tube with filter paper and let it dry naturally. The stainless steel rod mandrel is taken out to finally obtain a semi-permeable chitosan round tube with a wall thickness of 0.4mm.

[0028] (2) as shown in the accompanying drawing, the chitosan tube 6 that length is 1cm is placed in special mold ( figure 1 ) between the two steel needle...

Embodiment 2

[0030] (1) Immerse a stainless steel rod mandrel coated with polytetrafluoroethylene with a diameter of 5mm in 4% (w / v) chitosan solution (prepared with 0.2mol / L acetic acid solution), pull it out, freeze it, and then immerse and pull it out Take out, freeze, repeat 5 times, then freeze at -196°C for 48 hours, and then freeze-dry in a freeze dryer. The dried chitosan tube with mandrel is placed in 2% (w / v) sodium hydroxide solution and soaked for 30 minutes, taken out, rinsed to neutral with deionized water, then immersed in pH = 7.2 to 7.4 in phosphate buffered saline for 30 minutes. Absorb the moisture on the surface of the chitosan tube with filter paper and let it dry naturally. The stainless steel rod mandrel is taken out to finally obtain a semi-permeable chitosan round tube with a wall thickness of 1.0 mm.

[0031] (2) as shown in the accompanying drawing, the chitosan tube 6 that length is 10cm is placed in special mold ( figure 1 ) between the two steel needle fixi...

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Abstract

Disclosed is a nervous tissue engineering tubular supporting stand and method for making same, wherein the nervous tissue engineering tubular supporting stand comprises a chitosan pipe wall and biological source filling base material with axial multiple passages, and the method consists of preparing semipermeable chitosan hollow pipe with 1-5mm of inside diameter, pouring large biological molecule solvent such as chitosan, collagen or gelatine, and utilizing special-purpose die arrangement and lyophilization technology. The obtained multiple-pass nerve channel with bionic construction is beneficial for cell adhesion, migration and leading of neuraxon directional growth, and suitable of the renovation and regeneration of nerve damages.

Description

technical field [0001] The invention relates to a tubular support for neural tissue engineering and a preparation method thereof, in particular to a nerve conduit with an axial multi-channel specific microstructure matrix, a preparation method and a special mold thereof, belonging to the technical fields of biomedical materials and tissue engineering. Background technique [0002] Peripheral nerve injuries are very common clinically. Once the nerve is damaged, the sensory and motor functions of the corresponding parts will be affected or even lost. The current clinically effective treatment is autologous nerve transplantation. However, the source of autologous nerves is limited, and the function of the donor site is impaired. People try to use skeletal muscle and vein instead of autologous nerve graft, with little success. With the development of medicine, biology and tissue engineering, people consider and try to use artificial nerve guide instead of autologous nerve tran...

Claims

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Application Information

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IPC IPC(8): A61F2/04A61L27/00A61L27/20A61L27/24
Inventor 敖强王爱军张秀芳公衍道赵南明
Owner TSINGHUA UNIV
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