187 results about "Sulfation" patented technology

The invention discloses a preparation method and application of chondroitin sulfate sodium, and belongs to the technical field of chondroitin sulfate. The preparation method of the sodium chondroitin sulfate comprises the following steps: dispersing sodium chondroitin which is not sulfated by using an organic solvent, adding a sulfonating reagent for sulfonation reaction, terminating the reaction, centrifuging, and collecting precipitate; and decoloring and drying the collected precipitate to obtain the chondroitin sulfate sodium. The average molecular weight of the final product obtained by the method is controllable, the 6-site overall sulfonation rate is up to 90% or above, the 6-site independent sulfonation rate is up to 60%-85%, and the quality of products among batches is stable. The sodium chondroitin sulfate based on the characteristics is more suitable for being absorbed by a human body, high in bioavailability and higher in safety and effectiveness; the method can be realized without a complex extraction and purification process, and is rich in source, uniform in structure, stable in quality, mild in reaction, reasonable and controllable in production cycle and suitable for large-scale commercial production and application.

The invention provides a sulfonator for preparing fatty alcohol polyoxyethylene ether sodium sulfate and a production process of the sulfonator, relates to a novel falling film sulfonator, and belongs to the technical field of manufacturing of reaction devices for producing anionic surfactants by sulfating gas SO3. The invention provides a novel falling film sulfonator capable of being used for producing fatty alcohol polyoxyethylene ether sodium sulfate with ultralow dioxane content, the sulfonator improves the structure of a reaction tube, and a separation layer is formed between SO3 diluted gas and an organic material liquid film by introducing a balance wind concept, so that the content of the dioxane in the organic material liquid film is reduced, and the content of the dioxane in the organic material liquid film is reduced. According to the falling-film sulfonation tube, the sharp temperature interval at the upper part of the falling-film reactor tends to be mild, and the sulfonation / sulfation reaction rate in the length of the whole falling-film sulfonation tube tends to be balanced, so that the output of dioxane is reduced, the product quality is improved, the subsequent post-treatment of AES products is avoided, and the production energy consumption is reduced; the device is easy to operate and has certain practicability.

A lubricating composition includes an oil of lubricating viscosity, a phenolic antioxidant, a succinimide dispersant which is at least substantially free of boron, a detergent comprising an alkaline earth metal salicylate and a sulfurized organic compound.

The invention discloses a novel biosynthesis path for producing 3 '-phosphoadenosine-5'-phosphosulfuric acid and application, and belongs to the technical field of bioengineering. According to the invention, through recombinant expression and purification of ribose kinase, ribose phosphate pyrophosphate kinase, ribose phosphate transferase, adenine thiotransferase, adenosine phosphate sulfate kinase and polyphosphate kinase 2, a six-enzyme cascade reaction taking ribose and adenosine as substrates in vitro is constructed, the molar conversion rate reaches 22.85%, and the escherichia coli and bacillus subtilis PAPS cell factory can be used for preparing the recombinant ribose phosphate pyrophosphate kinase, the ribose phosphate transferase, the adenine thiotransferase, the adenosine phosphate sulfate kinase and the polyphosphate kinase 2. The yield of PAPS in a 5-L fermentation tank reaches 7.60 g / L and 5.03 g / L respectively. The PAPS synthesis route provided by the invention provides an extensible, economical and efficient solution for the production of sulfated compounds.

The invention relates to the technical field of fluorite flotation reagents, in particular to a preparation method of a fluorite flotation collecting agent. The specific preparation method comprises the following steps: carrying out sulfonation reaction on sulfuric acid and benzene to obtain sulfonation reaction liquid; the method comprises the following steps: by taking oleic acid or acid oil as a raw material, adding sulfonation reaction liquid at 10-20 DEG C, and carrying out stirring reaction; adding alkali liquor to carry out neutralization reaction, controlling the temperature to be less than or equal to 80 DEG C, and adjusting the pH value to be 7-8; and a water phase is separated and removed, and a mixture containing oleate, sulfated oleate, hydroxyl oleate and stearic acid benzene sulfonate is obtained and serves as the fluorite flotation collecting agent. By controlling the proportion of sulfuric acid to benzene and adding oleic acid, on the basis of reducing product benzene residues, the collecting capacity is improved, good selectivity is achieved, and the problem of aromatic hydrocarbon residues existing in a flotation collecting agent prepared from aromatic hydrocarbons such as benzene as raw materials is solved.

Biomaterials disclosed herein can comprise a hydrogel comprising PEG, gelatin, and a glycosaminoglycan with sulfated moiety; and chondrogenic, osteogenic, and immunomodulatory cytokines; wherein the biomaterial is capable of potentiating bone regeneration in a compromised wound while reducing inflammatory response. The glycosaminoglycan with sulfated moiety can comprise heparin, heparan sulfate, keratin sulfate, chondroitin sulfate, dermatan sulfate, and / or similar materials. The biomaterial can further comprise mesenchymal stem cells (MSCs), a crosslinking initiator, microparticles and nanoparticles, and or other materials. The biomaterial can be injectable into a wound, or the biomaterial can be loaded in, or further comprise a porous scaffold providing mechanical support for other components of the biomaterial, such that it can be implanted into a wound.

The invention discloses a preparation method and application of chondroitin sulfate sodium, and belongs to the technical field of chondroitin sulfate. The preparation method of the sodium chondroitin sulfate comprises the following steps: dispersing sodium chondroitin which is not sulfated by using an organic solvent, adding a sulfonating reagent for sulfonation reaction, terminating the reaction, centrifuging, and collecting precipitate; and decoloring and drying the collected precipitate to obtain the chondroitin sulfate sodium. The average molecular weight of the final product obtained by the method is controllable, the 6-site overall sulfonation rate is up to 90% or above, the 6-site independent sulfonation rate is up to 60%-85%, the quality of products among batches is stable, and sodium chondroitin sulfate based on the characteristics is more suitable for human body absorption, high in bioavailability and higher in safety and effectiveness; moreover, the method can realize abundant sources, uniform structure, stable quality, mild reaction and reasonable and controllable production cycle without complex extraction and purification processes, and is suitable for large-scale commercial production and application.

The invention discloses a polyampholyte chitosan with high polymerization degree and water solubility and a preparation method thereof, and belongs to the field of natural polymer material preparation. The polyampholyte chitosan is prepared by mixing aminosulfonic acid and one or more of glycerol, urea, lactic acid or choline chloride as a low eutectic solvent, and then adding chitosan for modification, and contains a polyampholyte of cationic groups and anionic groups. The chitosan sulfate product prepared by the invention has a small molecular weight loss, controllable modification sites, and can obtain a polyampholyte chitosan with high polymerization degree; the sulfation reaction has high efficiency, few side reactions, high product purity, good product water solubility, and extremely high application value.

The invention relates to heparinase III with high activity on 6-sulfated heparin and application of the heparinase III. The amino acid sequence of the heparinase III (BeHepIII) is as shown in SEQ ID NO. 2, and the nucleotide sequence of the coding gene is as shown in SEQ ID NO. 1. The invention discovers and discloses a novel heparinase III (BeHepIII) for the first time, the heparinase III is derived from Bacteroides eggerthii, has a higher recombinant expression level in escherichia coli, has very high degradation activity on heparin containing a 6-site sulfated structure, and particularly has the enzyme activity which is 93.5 times of that of commercialized heparinase FhHepIII at present, and the heparinase III can be used for degrading heparin containing 6-site sulfated structure. Therefore, the heparinase III (BeHepIII) provided by the invention has a wide application potential in heparin degradation. In addition, by using heparinase III (BeHepIII), directional excision of a reducing end of heparin oligosaccharide synthesized by a chemical enzyme method is realized, and complete chemical enzyme method synthesis of a pentasaccharide structure with anticoagulant factor Xa activity is successfully realized.

The invention belongs to the technical field of biological medicines, and relates to application of a PAPSS1 agonist and an endogenous protein sulfation modification up-regulating agent in preparation and screening of medicines for treating ischemic diseases. According to the application, the association between PAPSS1 up-regulation / excitation and histone sulfation modification mediated by PAPSS1 up-regulation / excitation and ischemic diseases is disclosed for the first time, and a new prevention and treatment thought is provided for ischemic cerebral apoplexy and other ischemic diseases.

The present invention relates to a process for the preparation of a compound of formula (I), a stereoisomer, a salt, a hydrate or a solvate thereof, (II) comprising the step of sulfinylation of a compound of formula (IV) to produce compound of formula (II); (IV) wherein: R1 and R2 are as defined in the claims, wherein said sulfinylation step is performed in the presence of a solvent selected from the group comprising benzene, toluene, ethylbenzene, xylene, chlorobenzene, bromobenzene, fluorobenzene, anisole, nitrobenzene, tetralin, dichlorobenzene, trimethylbenzene, ethylacetate, propylacetate, butylacetate, propanol, butanol, 2- methyltetrahydrofuran, diethylether, methyltertbutylether, cyclopentyl methyl ether, dichloromethane, tetrachloromethane, pentane, hexane, heptane, cyclohexane, decaline and mixtures thereof.

The invention discloses a functional lipid compound for nano-liposome wrapping and a preparation method, and relates to the technical field of functional lipid compounds, the functional lipid compound comprises (a) a polyphenol-lipid covalent conjugate which is composed of a polyphenol active unit selected from curcumin, dihydroquercetin or derivatives thereof, and (b) a lipid covalent conjugate which is composed of a polyphenols active unit selected from curcumin, dihydroquercetin or derivatives thereof and a polyphenols active unit selected from curcumin, dihydroquercetin or derivatives thereof; the conjugate is covalently linked to a phosphatidyl ethanolamine or cholesterol skeleton through a cleavable linking group, and the conjugate can be integrated into a nano-liposome membrane and has the functions of structural support and biological activity; (b) a microenvironment-responsive functional phospholipid, which is a phospholipid derivative having a functional group responsive to an inflammatory microenvironment specific stimulus modified on a hydrophilic head group or a hydrophobic chain of a phospholipid molecule, the specific stimulus being selected from local pH < = 6.5, reactive oxygen species (ROS) concentration > = 10 [mu] M or matrix metalloproteinase MMP-2 / 9 overexpression; and (c) a bionic exosome membrane lipid component, wherein the bionic exosome membrane lipid component comprises sphingosine-based glycolipid or sulfated cholesterol separated from the plant-derived exosome.

This invention provides an antiviral sulfated mulberry leaf oligosaccharide, its preparation method, animal feed, and applications, belonging to the field of oligosaccharide technology. The invention involves microwave degradation and H2O2-Vc degradation of mulberry leaf polysaccharides, followed by sulfation modification of the degradation products to obtain sulfated mulberry leaf oligosaccharides. Experiments have shown that adding sulfated mulberry leaf oligosaccharides to feed for aquaculture can promote the growth of largemouth bass, improve feed utilization, antioxidant capacity, lipid metabolism, immune function, and enhance resistance to viral infections.

The present invention relates to sulfonated and sulfated compounds obtainable from aryl aliphatic ketones, to a process for producing such compounds and to the use of these compounds as surfactants alone or in combination with other surfactants.

The invention discloses an acid circulation process in a process of sulfating and decomposing rare earth minerals, which comprises the following steps: 1) mixing the sulfated and decomposed rare earth minerals with slurry mixing liquid to obtain primary leaching residues A1 and an acid solution B containing rare earth; 2) mixing the rare earth-containing acidic solution B with an amine-containing extractant I to obtain a sulfuric acid-loaded organic solution C and a rare earth-containing raffinate D; 3) mixing the raffinate D with an acidic phosphorus type extractant II to obtain a rare earth-loaded organic solution X and raffinate E containing dilute sulphuric acid; 4) mixing the organic solution C loaded with the sulfuric acid with water to obtain an organic solution Y containing the extracting agent I and a sulfuric acid strip liquor Z; and (5) returning the raffinate E containing dilute sulphuric acid to the step (1), mixing the raffinate E with the primary leaching residue A1, and repeating the steps (1)-(5) for n-1 times in total. According to the method, recycling of sulfuric acid is achieved, and the use amount of water in the leaching process is reduced.

The application relates to the technical field of surfactants, in particular to a pentadecyl phenol alkoxyl ether sodium sulfate surfactant and a preparation method thereof. The preparation method of the pentadecyl phenol alkoxyl ether sodium sulfate surfactant comprises the following steps: after pretreatment, cashew nut shell liquid is selectively hydrogenated to obtain pentadecyl phenol with a specific composition; then, alkoxylization reaction is carried out under the condition of an alkaline catalyst to obtain pentadecyl phenol polyalkoxyl ether; and the pentadecyl phenol polyalkoxyl ether is sulfated and neutralized to obtain the pentadecyl phenol alkoxyl ether sodium sulfate surfactant. The surfactant improves the problem that the activity of the surfactant is reduced when the saturation of the pentadecyl phenol is too high or too low; ensures that the final product has good fluidity at low temperature, guarantees the continuity of production, effectively reduces the occurrence of over-sulfation; and simultaneously improves the production efficiency and reduces the cost.

The present invention discloses a Sepia esculenta ink polysaccharide for improving high-glucose damage of endothelial cells, and a preparation method and application thereof. The Sepia esculenta ink polysaccharide has a polysaccharide backbone composed of fucose, galactosamine, mannose, and N-acetylglucosamine, and has a glucuronic acid branch chain at the C-3 position of mannose. It is an aminopolysaccharide with a weight-average molecular weight of 10-16 kDa and a sulfation degree of 8%-15%. Through experimental verification, compared with similar compounds, the Sepia esculenta ink polysaccharide can effectively improve the state of endothelial cells and maintain the normal filtration function of vascular endothelium. At the same time, the risk of bleeding side effects of the Sepia esculenta ink polysaccharide is significantly lower than that of similar polysaccharides, and it has higher safety in use. Furthermore, the Sepia esculenta ink polysaccharide can be used to develop adjuvant therapeutic drugs for late-stage diabetic lesions, filling the gap in such products on the current market.

The invention relates to a microbial growth activation accelerant based on rare earth elements as well as a preparation method and application thereof, and belongs to the field of resources and environments. The microbial growth activation accelerant based on the rare earth elements is prepared from the following components: 1 to 150g of a rare earth compound, 0.2 to 2g of pantothenic acid, 0.2 to 2g of nicotinic acid, 0.02 to 0.1 g of nicotinamide and 1000g of deionized water, the rare earth compound is one or combination of more of chlorides of lanthanum, cerium, praseodymium, neodymium, promethium, samarium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium, lutetium, scandium and yttrium, nitric acid compounds and sulfuric acid compounds. A large number of cheap light rare earth elements are used as raw materials of the microbial accelerator, a new utilization approach is provided for the light rare earth elements, the light rare earth elements are converted into high-added-value products, pollution of the light rare earth elements to the environment is reduced, and meanwhile new elements are introduced into the field of microbial accelerators. More importantly, the preparation method of the microbial growth activation accelerant based on the rare earth elements provided by the invention is simple in production process and convenient for large-scale production.

The application belongs to the technical field of chondroitin sulfate preparation, and particularly relates to application and application method of liquid sulfur dioxide in preparation of chondroitin sulfate or chondroitin sulfate salt. In the application, liquid sulfur dioxide is used as a solvent to perform sulfonation modification treatment on unsulfated chondroitin, on the one hand, the use of traditional high-boiling organic reagents is avoided, the difficulty of three-waste treatment is reduced, and the process is more green and environmentally friendly; on the other hand, the post-treatment operation of the sulfonation reaction is simpler, and sulfur dioxide can be removed from the system by only temperature control or pressure adjustment, so that solvent residues possibly existing in the final product are avoided, and the product is safer. In addition, by adjusting the key conditions of the sulfonation reaction, the proportion of chondroitin sulfate with 6 independent sulfation in the disaccharide unit of the sulfonation product is 70% to 90%, the reaction is stable and reliable, and the sulfonation rate is high.

The invention discloses a method for producing sterols and sulfated derivatives thereof through fermentation and improving sulfation degree, and belongs to the field of bioengineering. The yeast engineering bacteria for fermentation production of sterols and sulfated derivatives thereof are constructed by taking yeast as an original strain and knocking out and introducing genes. The engineered yeast can efficiently produce 7-dehydrocholesterol and cholesterol, especially cholesterol sulfate, the shake flask fermentation yield of the engineered yeast is close to 249.0 mg / L, and the fermentation yield of a 5L fermentation tank can reach 544.7 mg / L. By highly expressing key rate-limiting enzyme in a metabolic pathway, enhancing precursor supply and enhancing cofactor PAPS supply, the yield and sulfation degree of the cholesterol sulfate are improved, and the pichia pastoris is utilized to efficiently generate the cholesterol sulfate, so that the method has the characteristics of short period, environmental protection and resource conservation. The yeast engineering bacterium for high-yield production of cholesterol sulfate has a wide application prospect, and provides important reference for production of other sulfated products.

The invention belongs to the technical field of metal battery negative electrode materials, and particularly discloses a sulfur-doped hard carbon material and a preparation method and application thereof. The preparation method of the sulfur-doped hard carbon material comprises the following steps: dissolving chitosan in methanesulfonic acid, and uniformly mixing; adding into diethyl ether until white powder is hydrophobically dissolved out; then removing the residual solvent in the white powder to obtain sulfated chitosan; and finally calcining the sulfated chitosan to obtain the product. A sulfur oxyacid radical molecular template method is adopted, a template is effectively anchored on a biomass chain through a chemical reaction between the template and biomass, in the subsequent high-temperature pyrolysis process, the molecular template is decomposed and self-sacrifice, sub-nano pores are reserved in situ, and effective heteroatom doping is further carried out on the surfaces of the pores. When the composite material is applied to a sodium ion battery as a negative electrode, the occurrence of electrolyte-carbon negative electrode interface side reaction can be effectively inhibited, reversible adsorption and desorption of sodium ions in a carbon matrix are promoted, and extremely high first-circle coulombic efficiency can be shown and can reach 100%.

The present invention relates to the use of optionally sulfated or sulfonated gallic acid esters or amides or optionally sulfated hydrogenated gallic esters or amides as surfactants, to surfactant compositions comprising such compounds, to sulfonated and optionally also sulfated gallic acid esters or amides, to sulfated hydrogenated gallic esters or amides and to methods for preparing such compounds.

The invention relates to the technical field of sodium chondroitin sulfate, in particular to sodium chondroitin sulfate with a high cartilage repair effect and a preparation method thereof.The sodium chondroitin sulfate initial feed liquid is provided, the pH value of the sodium chondroitin sulfate initial feed liquid is adjusted to a preset acidic pH value, and at the preset reaction temperature, the pH value and the reaction temperature are maintained for reaction time, so that the chondroitin sulfate with the high cartilage repair effect is obtained. The method comprises the following steps: adding a sulfated disaccharide unit CS-0 into a feed liquid to precipitate out a non-sulfated disaccharide unit CS-0 in the feed liquid, carrying out solid-liquid separation on the feed liquid, and removing the CS-0 precipitate to obtain a supernatant; pumping the supernatant into a gradient membrane separation system under controlled transmembrane pressure difference and feed liquid temperature, and separating the supernatant into three component flows with different molecular weight intervals; the concentration of the three component flows is monitored, the volume flow of each component flow is dynamically adjusted according to the preset molecular weight and mass distribution proportion, the chondroitin sulfate sodium product with the fixed molecular weight distribution is obtained, and the biological activity and the application value of the chondroitin sulfate sodium are improved.

The invention relates to the technical field of enzymatic hydrolysis, and particularly to an enzymatic hydrolysis preparation method for low-molecular-weight heparin sodium. The method comprises the following steps: S1.1, pre-treating the sodium heparin and combining it with a protective agent to obtain a heparin complex precipitate; S1.2, performing a first enzymatic hydrolysis on the heparin complex precipitate to obtain a supernatant A and a precipitate; S1.3, adjusting the pH of the precipitate to release highly sulfated fragments to obtain a supernatant B; S1.4, performing a second enzymatic hydrolysis on the supernatant B to obtain a secondary enzymatic hydrolyzate, combining the supernatant A and the secondary enzymatic hydrolyzate, and filtering to obtain a retentate; S1.5, post-treating the retentate to obtain the low-molecular-weight heparin sodium; and using a composite protective agent prepared by covalently coupling citric acid-modified superparamagnetic Fe3O4 nanoparticles with aminocalixarene, accurately regulating the enzymatic hydrolysis path and achieving efficient separation, thereby solving the problems of uneven molecular weight, low yield, and complex purification in traditional processes.

This invention discloses a method for preparing dodecanoic acid lactam, comprising an oximation reaction of cyclododecanone, a rearrangement reaction of cyclododecanone oxime, and extraction and purification. The oximation reaction of this invention uses a cobalt-based MOF catalyst, which has high catalytic activity and can significantly improve the conversion rate of the cyclododecanone oximation reaction, thereby improving the overall reaction efficiency, reducing the formation of by-products, and thus improving the purity of the target product. In the rearrangement reaction of cyclododecanone oxime, zirconium oxide sulfate is used as a catalyst. This catalyst has good catalytic performance under high temperature conditions and can effectively promote the rearrangement reaction. Simultaneously, the ionic liquid is used as a solvent, which not only has good solubility but is also environmentally friendly and easy to recycle, reducing production costs and environmental impact.

This invention belongs to the field of surfactant preparation technology, specifically relating to a method for preparing a sulfate ester salt type surfactant. The method includes the following steps: olefins and concentrated sulfuric acid are separately introduced into a microchannel reactor equipped with a catalyst section and mixed and reacted under ultrasonic conditions to obtain an intermediate product; an aqueous solution of NaOH or KOH is added to the intermediate product, the temperature is raised to 90-105℃, and the mixture is stirred and refluxed for 1-2 hours to obtain the sulfate ester salt type surfactant. This invention combines a microchannel reactor with fine chemical synthesis and successfully synthesizes secondary alkyl sulfates. By accelerating the reaction with a catalyst and ultrasound, increasing the phase contact area, and controlling the reaction temperature at a low level, the degree of olefin sulfation is greatly improved, thereby increasing the yield of secondary alkyl sulfates. The method is safe, environmentally friendly, easy to operate, simple in process, and easy to control. It is expected that production can be scaled up to industrial scale through array arrangement.

The present disclosure relates to disaccharides with defined sulfation patterns, and oligosaccharide mimetics comprising the disaccharides as repeating units linked in a head to tail fashion. The present disclosure further relates to methods of making the same, and to methods of using the same to mediate cell proliferation, cell differentiation, amyloid plaque formation, anti-coagulation, and neuronal growth.

The invention discloses a method for analyzing a pharmacodynamic material basis and metabolites of Longqing capsules. According to the method, an UPLC-Q-Exactive-Orbitrap MS technology is adopted, under the same chromatographic condition and mass spectrum condition, the pharmacodynamic material basis and metabolites of the Longqing capsules are measured and analyzed, 128 compounds are analyzed from the Longqing capsules, 144 exogenous compounds including 61 prototype components and 83 metabolites are analyzed from plasma, urine and excrement of rats in total, and the pharmacodynamic material basis and the metabolites of the Longqing capsules are analyzed to form the Longqing capsules with the pharmacodynamic material basis and the metabolites of the Longqing capsules with the pharmacodynamic material basis and the metabolites of the Longqing capsules with the pharmacodynamic material basis and the metabolites of the Longqing capsules. The main metabolic pathways comprise oxidation, reduction, hydrolysis, methylation, sulfation, glucuronization, composite reactions of various reaction types and the like. The invention provides a scientific basis for quality control and clinical reasonable medication of Longqing capsule medicines.

The invention discloses the preparation and application of a class of nonionic amphiphilic compounds with a mixed structure, and belongs to the field of fine chemical technology. The compound of the present invention is formed by adding propylene oxide and ethylene oxide to gubetainol or glycerol ether with a dialkyl structure, wherein the addition of propylene oxide and ethylene oxide adopts a mixed mode. The nonionic compound with a mixed structure of the present invention is a transparent liquid with low viscosity, and can be used as an intermediate, in the absence of solvent or in the presence of very little solvent, by a SO3 gas membrane sulfation process to further derive a nonionic anionic composite hydrophilic type surfactant, i.e., an alcohol ether sulfate type surfactant, which is particularly suitable for use as a salt-resistant oil displacement surfactant. The obtained surfactant can significantly change the phase state and properties of the oil / water interface, and is easy to reduce the oil / water interfacial tension to an ultra-low level.

The present invention relates to a method of purifying whole HCV particles, the method comprising the steps of a) providing a cell culture supernatant comprising virus particles, b) purification and / or concentration of the cell culture supernatant, c) purification and / or concentration of the product of above step b) using steric exclusion chromatography (SXC) at alkaline pH in the range of 8-10, d) purification and / or concentration of the product of the above step c) using sulphated cellulose membrane absorbers (SCMA), e) obtaining whole virus particles.