117 results about "Sulfation" patented technology

The invention discloses a novel biosynthesis path for producing 3 '-phosphoadenosine-5'-phosphosulfuric acid and application, and belongs to the technical field of bioengineering. According to the invention, through recombinant expression and purification of ribose kinase, ribose phosphate pyrophosphate kinase, ribose phosphate transferase, adenine thiotransferase, adenosine phosphate sulfate kinase and polyphosphate kinase 2, a six-enzyme cascade reaction taking ribose and adenosine as substrates in vitro is constructed, the molar conversion rate reaches 22.85%, and the escherichia coli and bacillus subtilis PAPS cell factory can be used for preparing the recombinant ribose phosphate pyrophosphate kinase, the ribose phosphate transferase, the adenine thiotransferase, the adenosine phosphate sulfate kinase and the polyphosphate kinase 2. The yield of PAPS in a 5-L fermentation tank reaches 7.60 g / L and 5.03 g / L respectively. The PAPS synthesis route provided by the invention provides an extensible, economical and efficient solution for the production of sulfated compounds.

Biomaterials disclosed herein can comprise a hydrogel comprising PEG, gelatin, and a glycosaminoglycan with sulfated moiety; and chondrogenic, osteogenic, and immunomodulatory cytokines; wherein the biomaterial is capable of potentiating bone regeneration in a compromised wound while reducing inflammatory response. The glycosaminoglycan with sulfated moiety can comprise heparin, heparan sulfate, keratin sulfate, chondroitin sulfate, dermatan sulfate, and / or similar materials. The biomaterial can further comprise mesenchymal stem cells (MSCs), a crosslinking initiator, microparticles and nanoparticles, and or other materials. The biomaterial can be injectable into a wound, or the biomaterial can be loaded in, or further comprise a porous scaffold providing mechanical support for other components of the biomaterial, such that it can be implanted into a wound.

The invention discloses a functional lipid compound for nano-liposome wrapping and a preparation method, and relates to the technical field of functional lipid compounds, the functional lipid compound comprises (a) a polyphenol-lipid covalent conjugate which is composed of a polyphenol active unit selected from curcumin, dihydroquercetin or derivatives thereof, and (b) a lipid covalent conjugate which is composed of a polyphenols active unit selected from curcumin, dihydroquercetin or derivatives thereof and a polyphenols active unit selected from curcumin, dihydroquercetin or derivatives thereof; the conjugate is covalently linked to a phosphatidyl ethanolamine or cholesterol skeleton through a cleavable linking group, and the conjugate can be integrated into a nano-liposome membrane and has the functions of structural support and biological activity; (b) a microenvironment-responsive functional phospholipid, which is a phospholipid derivative having a functional group responsive to an inflammatory microenvironment specific stimulus modified on a hydrophilic head group or a hydrophobic chain of a phospholipid molecule, the specific stimulus being selected from local pH < = 6.5, reactive oxygen species (ROS) concentration > = 10 [mu] M or matrix metalloproteinase MMP-2 / 9 overexpression; and (c) a bionic exosome membrane lipid component, wherein the bionic exosome membrane lipid component comprises sphingosine-based glycolipid or sulfated cholesterol separated from the plant-derived exosome.

This invention provides an antiviral sulfated mulberry leaf oligosaccharide, its preparation method, animal feed, and applications, belonging to the field of oligosaccharide technology. The invention involves microwave degradation and H2O2-Vc degradation of mulberry leaf polysaccharides, followed by sulfation modification of the degradation products to obtain sulfated mulberry leaf oligosaccharides. Experiments have shown that adding sulfated mulberry leaf oligosaccharides to feed for aquaculture can promote the growth of largemouth bass, improve feed utilization, antioxidant capacity, lipid metabolism, immune function, and enhance resistance to viral infections.

The invention discloses an acid circulation process in a process of sulfating and decomposing rare earth minerals, which comprises the following steps: 1) mixing the sulfated and decomposed rare earth minerals with slurry mixing liquid to obtain primary leaching residues A1 and an acid solution B containing rare earth; 2) mixing the rare earth-containing acidic solution B with an amine-containing extractant I to obtain a sulfuric acid-loaded organic solution C and a rare earth-containing raffinate D; 3) mixing the raffinate D with an acidic phosphorus type extractant II to obtain a rare earth-loaded organic solution X and raffinate E containing dilute sulphuric acid; 4) mixing the organic solution C loaded with the sulfuric acid with water to obtain an organic solution Y containing the extracting agent I and a sulfuric acid strip liquor Z; and (5) returning the raffinate E containing dilute sulphuric acid to the step (1), mixing the raffinate E with the primary leaching residue A1, and repeating the steps (1)-(5) for n-1 times in total. According to the method, recycling of sulfuric acid is achieved, and the use amount of water in the leaching process is reduced.

The application relates to the technical field of surfactants, in particular to a pentadecyl phenol alkoxyl ether sodium sulfate surfactant and a preparation method thereof. The preparation method of the pentadecyl phenol alkoxyl ether sodium sulfate surfactant comprises the following steps: after pretreatment, cashew nut shell liquid is selectively hydrogenated to obtain pentadecyl phenol with a specific composition; then, alkoxylization reaction is carried out under the condition of an alkaline catalyst to obtain pentadecyl phenol polyalkoxyl ether; and the pentadecyl phenol polyalkoxyl ether is sulfated and neutralized to obtain the pentadecyl phenol alkoxyl ether sodium sulfate surfactant. The surfactant improves the problem that the activity of the surfactant is reduced when the saturation of the pentadecyl phenol is too high or too low; ensures that the final product has good fluidity at low temperature, guarantees the continuity of production, effectively reduces the occurrence of over-sulfation; and simultaneously improves the production efficiency and reduces the cost.

The invention relates to a microbial growth activation accelerant based on rare earth elements as well as a preparation method and application thereof, and belongs to the field of resources and environments. The microbial growth activation accelerant based on the rare earth elements is prepared from the following components: 1 to 150g of a rare earth compound, 0.2 to 2g of pantothenic acid, 0.2 to 2g of nicotinic acid, 0.02 to 0.1 g of nicotinamide and 1000g of deionized water, the rare earth compound is one or combination of more of chlorides of lanthanum, cerium, praseodymium, neodymium, promethium, samarium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium, lutetium, scandium and yttrium, nitric acid compounds and sulfuric acid compounds. A large number of cheap light rare earth elements are used as raw materials of the microbial accelerator, a new utilization approach is provided for the light rare earth elements, the light rare earth elements are converted into high-added-value products, pollution of the light rare earth elements to the environment is reduced, and meanwhile new elements are introduced into the field of microbial accelerators. More importantly, the preparation method of the microbial growth activation accelerant based on the rare earth elements provided by the invention is simple in production process and convenient for large-scale production.

The application belongs to the technical field of chondroitin sulfate preparation, and particularly relates to application and application method of liquid sulfur dioxide in preparation of chondroitin sulfate or chondroitin sulfate salt. In the application, liquid sulfur dioxide is used as a solvent to perform sulfonation modification treatment on unsulfated chondroitin, on the one hand, the use of traditional high-boiling organic reagents is avoided, the difficulty of three-waste treatment is reduced, and the process is more green and environmentally friendly; on the other hand, the post-treatment operation of the sulfonation reaction is simpler, and sulfur dioxide can be removed from the system by only temperature control or pressure adjustment, so that solvent residues possibly existing in the final product are avoided, and the product is safer. In addition, by adjusting the key conditions of the sulfonation reaction, the proportion of chondroitin sulfate with 6 independent sulfation in the disaccharide unit of the sulfonation product is 70% to 90%, the reaction is stable and reliable, and the sulfonation rate is high.

The invention belongs to the technical field of metal battery negative electrode materials, and particularly discloses a sulfur-doped hard carbon material and a preparation method and application thereof. The preparation method of the sulfur-doped hard carbon material comprises the following steps: dissolving chitosan in methanesulfonic acid, and uniformly mixing; adding into diethyl ether until white powder is hydrophobically dissolved out; then removing the residual solvent in the white powder to obtain sulfated chitosan; and finally calcining the sulfated chitosan to obtain the product. A sulfur oxyacid radical molecular template method is adopted, a template is effectively anchored on a biomass chain through a chemical reaction between the template and biomass, in the subsequent high-temperature pyrolysis process, the molecular template is decomposed and self-sacrifice, sub-nano pores are reserved in situ, and effective heteroatom doping is further carried out on the surfaces of the pores. When the composite material is applied to a sodium ion battery as a negative electrode, the occurrence of electrolyte-carbon negative electrode interface side reaction can be effectively inhibited, reversible adsorption and desorption of sodium ions in a carbon matrix are promoted, and extremely high first-circle coulombic efficiency can be shown and can reach 100%.

The present invention relates to the use of optionally sulfated or sulfonated gallic acid esters or amides or optionally sulfated hydrogenated gallic esters or amides as surfactants, to surfactant compositions comprising such compounds, to sulfonated and optionally also sulfated gallic acid esters or amides, to sulfated hydrogenated gallic esters or amides and to methods for preparing such compounds.

The invention relates to the technical field of sodium chondroitin sulfate, in particular to sodium chondroitin sulfate with a high cartilage repair effect and a preparation method thereof.The sodium chondroitin sulfate initial feed liquid is provided, the pH value of the sodium chondroitin sulfate initial feed liquid is adjusted to a preset acidic pH value, and at the preset reaction temperature, the pH value and the reaction temperature are maintained for reaction time, so that the chondroitin sulfate with the high cartilage repair effect is obtained. The method comprises the following steps: adding a sulfated disaccharide unit CS-0 into a feed liquid to precipitate out a non-sulfated disaccharide unit CS-0 in the feed liquid, carrying out solid-liquid separation on the feed liquid, and removing the CS-0 precipitate to obtain a supernatant; pumping the supernatant into a gradient membrane separation system under controlled transmembrane pressure difference and feed liquid temperature, and separating the supernatant into three component flows with different molecular weight intervals; the concentration of the three component flows is monitored, the volume flow of each component flow is dynamically adjusted according to the preset molecular weight and mass distribution proportion, the chondroitin sulfate sodium product with the fixed molecular weight distribution is obtained, and the biological activity and the application value of the chondroitin sulfate sodium are improved.

This invention discloses a method for preparing dodecanoic acid lactam, comprising an oximation reaction of cyclododecanone, a rearrangement reaction of cyclododecanone oxime, and extraction and purification. The oximation reaction of this invention uses a cobalt-based MOF catalyst, which has high catalytic activity and can significantly improve the conversion rate of the cyclododecanone oximation reaction, thereby improving the overall reaction efficiency, reducing the formation of by-products, and thus improving the purity of the target product. In the rearrangement reaction of cyclododecanone oxime, zirconium oxide sulfate is used as a catalyst. This catalyst has good catalytic performance under high temperature conditions and can effectively promote the rearrangement reaction. Simultaneously, the ionic liquid is used as a solvent, which not only has good solubility but is also environmentally friendly and easy to recycle, reducing production costs and environmental impact.

This invention belongs to the field of surfactant preparation technology, specifically relating to a method for preparing a sulfate ester salt type surfactant. The method includes the following steps: olefins and concentrated sulfuric acid are separately introduced into a microchannel reactor equipped with a catalyst section and mixed and reacted under ultrasonic conditions to obtain an intermediate product; an aqueous solution of NaOH or KOH is added to the intermediate product, the temperature is raised to 90-105℃, and the mixture is stirred and refluxed for 1-2 hours to obtain the sulfate ester salt type surfactant. This invention combines a microchannel reactor with fine chemical synthesis and successfully synthesizes secondary alkyl sulfates. By accelerating the reaction with a catalyst and ultrasound, increasing the phase contact area, and controlling the reaction temperature at a low level, the degree of olefin sulfation is greatly improved, thereby increasing the yield of secondary alkyl sulfates. The method is safe, environmentally friendly, easy to operate, simple in process, and easy to control. It is expected that production can be scaled up to industrial scale through array arrangement.

The invention discloses a method for analyzing a pharmacodynamic material basis and metabolites of Longqing capsules. According to the method, an UPLC-Q-Exactive-Orbitrap MS technology is adopted, under the same chromatographic condition and mass spectrum condition, the pharmacodynamic material basis and metabolites of the Longqing capsules are measured and analyzed, 128 compounds are analyzed from the Longqing capsules, 144 exogenous compounds including 61 prototype components and 83 metabolites are analyzed from plasma, urine and excrement of rats in total, and the pharmacodynamic material basis and the metabolites of the Longqing capsules are analyzed to form the Longqing capsules with the pharmacodynamic material basis and the metabolites of the Longqing capsules with the pharmacodynamic material basis and the metabolites of the Longqing capsules with the pharmacodynamic material basis and the metabolites of the Longqing capsules. The main metabolic pathways comprise oxidation, reduction, hydrolysis, methylation, sulfation, glucuronization, composite reactions of various reaction types and the like. The invention provides a scientific basis for quality control and clinical reasonable medication of Longqing capsule medicines.

The present invention relates to a method of purifying whole HCV particles, the method comprising the steps of a) providing a cell culture supernatant comprising virus particles, b) purification and / or concentration of the cell culture supernatant, c) purification and / or concentration of the product of above step b) using steric exclusion chromatography (SXC) at alkaline pH in the range of 8-10, d) purification and / or concentration of the product of the above step c) using sulphated cellulose membrane absorbers (SCMA), e) obtaining whole virus particles.

Biomaterials disclosed herein can comprise a hydrogel comprising PEG, gelatin, and a glycosaminoglycan with sulfated moiety; and chondrogenic, osteogenic, and immunomodulatory cytokines; wherein the biomaterial is capable of potentiating bone regeneration in a compromised wound while reducing inflammatory response. The glycosaminoglycan with sulfated moiety can comprise heparin, heparan sulfate, keratin sulfate, chondroitin sulfate, dermatan sulfate, and / or similar materials. The biomaterial can further comprise mesenchymal stem cells (MSCs), a crosslinking initiator, microparticles and nanoparticles, and / or other materials. The biomaterial can be injectable into a wound, or the biomaterial can be loaded in, or further comprise a porous scaffold providing mechanical support for other components of the biomaterial, such that it can be implanted into a wound.

A water treatment system includes a water stream configured to provide raw water to be treated, a coagulant source configured to hold one or more sulfated coagulants, and one or more photoreactors configured to activate the one or more sulfated coagulants to produce an activated sulfated coagulant and configured to add the activated sulfated coagulant to the water stream within a determined period. Methods of water treatment are also disclosed.

The invention relates to the technical field of surfactants, in particular to a pentadecyl phenol alkoxy ether sodium sulfate surfactant and a preparation method thereof. The invention relates to a preparation method of a pentadecyl phenol alkoxy ether sodium sulfate surfactant. The preparation method comprises the following steps: pretreating cashew nut shell liquid, and selectively hydrogenating to obtain pentadecyl phenol with specific composition; carrying out an alkoxylation reaction in the presence of a basic catalyst to obtain pentadecyl phenol polyalkoxy ether; and sulfating the pentadecyl phenol polyalkoxy ether, and neutralizing to obtain the pentadecyl phenol alkoxy ether sodium sulfate surfactant. According to the surfactant, the problem that the activity of the surfactant is reduced due to too high or too low saturation of pentadecyl phenol is solved; it is ensured that the final product has good fluidity at low temperature, production continuity is guaranteed, and oversulfation is effectively reduced; meanwhile, the production efficiency is improved, and the cost is reduced.

The application discloses a lithium extraction method and equipment, and aims at solving the technical problems of long process and serious environmental pollution in the sulfuric acid method. The method comprises the following steps: through mixing a first material with a sulfate ion-based acidifying agent to perform a sulfation reaction, the first material is converted into a second material containing lithium sulfate, the first material contains a lithium-based compound, and the lithium sulfate is obtained from the lithium-based compound through the sulfation reaction; after or at the same time of converting the first material into the second material containing lithium sulfate by mixing the first material with the sulfate ion-based acidifying agent to perform the sulfation reaction, the second material is calcined to fully perform the sulfation reaction and remove the sulfate ion-based acidifying agent which is not completely reacted; the lithium sulfate in the second material is gasified and volatilized from the second solid phase material by heating the second material, and the solid phase material remaining after the lithium sulfate in the second material is gasified and volatilized is a third material.

This invention discloses a fucose polymer, its preparation method, and its applications. Using natural fucoidan sulfate ester as a template, a fucose polymer with well-defined and homogeneous structures is prepared via an olefinic metathesis ring-opening polymerization reaction. The structure involves different glycosidic bond linkages, degrees of polymerization, sugar chain lengths, and sulfate sites. The amphiphilic fucose polymer self-assembles into spherical nanoparticles, exhibiting inhibitory activity against type I herpes simplex virus infection. The sulfated fucoidan polymer with a degree of polymerization of 20 shows the best antiviral effect. This invention achieves efficient and controllable synthesis of fucoidan oligosaccharide polymers and has the potential value as a novel anti-herpes simplex virus drug, which can be applied in the preparation of anti-herpes simplex virus drugs.

This invention discloses a flotation separation method for removing talc from molybdenite using sodium alginate sulfate. The method involves preparing a raw slurry from talc-type molybdenite, controlling the pH of the slurry to be 6.0–10.0. Sodium alginate sulfate is added to the slurry and stirred. Then, an anisopolar collector is added and stirred to condition the slurry. A frother is then added to obtain a mixed slurry. Finally, the mixed slurry is subjected to aerated flotation. The collected frothy product is the molybdenum concentrate, and the product in the tank is the talc-containing tailings. This invention, by adding sodium alginate sulfate (SAS), which has high electronegativity and strong chelating ability, as a specific inhibitor to the flotation system, blocks the contact between the collector and talc. Simultaneously, its strong electrostatic repulsion effectively prevents non-specific adsorption on the negatively charged molybdenite surface. This invention significantly improves the grade and recovery rate of molybdenum concentrate and has extremely high industrial promotion and engineering application value.

There is provided a process for synthesizing a sulfated glycoarchaeal of Formula (I) where R1 is SO3M with M being an alkali metal, and n is 0 or 1; comprising: reacting a compound of Formula (A) with a sulfur trioxide reagent to form an intermediate compound; salification of the intermediate compound to form the compound of Formula (I).

The invention belongs to the technical field of plant polysaccharide, and discloses modified acanthopanax polysaccharide as well as a preparation method and application thereof. Acanthopanax senticosus is taken as a raw material, acanthopanax senticosus polysaccharide ASPS is extracted through a microwave-enzyme synergistic extraction method, ASPS-I, ASPS-II and ASPS-III are obtained through separation and purification of a DEAE-650M cellulose chromatographic column, and selenized acanthopanax senticosus polysaccharide or sulfated acanthopanax senticosus polysaccharide is obtained through chemical modification. The chemical modification process is simple and convenient, high in safety and few in by-products, organic solvents are not used in the whole process, and the green chemistry principle is met. The eleutherococcus senticosus selenized polysaccharides SeASPS-I, SeASPS-II and SeASPS-III disclosed by the invention, or the eleutherococcus senticosus sulfated polysaccharides sASPS-I, sASPS-II and sASPS-III disclosed by the invention can be used for effectively improving the motion state of BR5270 nematodes.

The invention relates to a polycarboxylic acid dispersing agent and a preparation method thereof, and the polycarboxylic acid dispersing agent is prepared from the following raw materials in parts by weight: 28-32 parts of sodium methylallyl sulfonate; 170 to 190 parts of polyether monomer; 8-12 parts of a modified amide monomer; 16 to 18 parts of unsaturated acid monomer; 1.8 to 2.2 parts of an oxidizing agent; 1.8 to 2.2 parts of a reducing agent; 2.2 to 2.8 parts of a chain transfer agent; and water; the polyether monomer is prepared from the following raw materials: phosphate ester, ethylene oxide and epoxypropane; the modified amide monomer is obtained by modifying N-hydroxymethyl acrylamide with a sulfating agent. According to the invention, the modified amide monomer containing a strong polar sulfate group and the polyether monomer with a specific EO / PO chain segment ratio are copolymerized, so that the synergistic effect of'reinforced anchoring 'and'steric hindrance' is realized, the defects of unfirm anchoring and insufficient steric hindrance of a traditional dispersant are overcome, the adsorption stability of the dispersant on the surface of a pigment is obviously enhanced, and the dispersion is more stable. Therefore, the viscosity of the coating is obviously reduced, and the fluidity of the coating is improved.

The invention relates to the technical field of environmental governance and functional materials, in particular to a codonopsis pilosula polysaccharide derivative as well as a preparation method and application thereof. The codonopsis pilosula polysaccharide derivative is mainly prepared from codonopsis pilosula polysaccharide through carboxymethylation or sulfation modification reaction. The codonopsis pilosula polysaccharide derivative is prepared on the basis of natural codonopsis pilosula polysaccharide through simple process steps, and the codonopsis pilosula polysaccharide derivative is especially used for adsorbing and enriching divalent lead ions as a biological adsorbent, so that the problems of high toxicity, high cost, complex adsorption material synthesis process and the like in a lead treatment method in the prior art are solved.

Surface‑bound procatalysts for hydrogenolysis of a polyolefin feed include an acidic sulfated support and cationic transition metal complexes M+Ly surface‑bound to at least one surface sulfate site QS − of the acidic sulfated support. The transition metal M is selected from the group consisting of Fe, Ni, Co, Cu, and Zn. The y number of ligands is 1, 2, or 3. Each ligand is a monodentate or multi‑dentate ligand coordinated to the transition metal with a hapticity greater than 1. Methods for hydrogenolysis of a polyolefin feed include contacting the surface‑bound procatalyst with a hydrogen feed to form active catalyst sites comprising hydride complexes M+H each surface‑bound to at least one anionic site of the sulfated support; contacting the active catalyst sites with the polyolefin feed in the presence of hydrogen; and allowing the polyolefin feed to react in the reactor to obtain a hydrogenolyzed product.

The invention discloses a microsphere core-shell structure catalyst and a preparation method and application thereof in carbonyl sulfide hydrolyzing.The preparation method of the microsphere core-shell structure catalyst comprises the following steps that cobalt salt, nickel salt, urea and a first solvent are evenly mixed to form a clear solution, the clear solution is subjected to a hydrothermal reaction at the temperature of 100-150 DEG C, a wet solid product is obtained, and the microsphere core-shell structure catalyst is obtained. And drying the wet solid product to obtain an inner core, uniformly mixing the inner core, cetyltrimethylammonium bromide and a second solvent, adding ammonia water under a stirring condition until the pH is 12-13, dropwise adding tetraethyl orthosilicate, reacting at room temperature under a stirring condition for 12-20 hours after dropwise adding is completed, centrifuging after the reaction is completed to obtain a precipitate, washing the precipitate to be neutral, and drying, so as to obtain the catalyst. The microsphere core-shell structure catalyst is obtained. The microsphere core-shell structure catalyst can effectively prevent sulfur and sulfation on the surface of the catalyst, so that the stability of the catalyst is remarkably improved while the catalytic activity is guaranteed.

The application provides oligosaccharide, a preparation method thereof and application thereof in aquatic feed, and belongs to the technical field of plant polysaccharide. The mulberry polysaccharide is sequentially subjected to pectinase enzymolysis, H2O2-Vc degradation and microwave degradation, the degradation product obtained is subjected to sulfation modification, and sulfated mulberry oligosaccharide is obtained. The sulfated mulberry oligosaccharide has the effects of improving the antioxidant capacity of aquatic animals, improving the antiviral property of aquatic animals, improving the immune capacity of aquatic animals and improving the growth performance of aquatic animals, and can be used as a feed additive in aquatic breeding.

This invention provides a modified fucoidan composite hydrogel, its preparation method, and its application, belonging to the field of hydrogel technology. The preparation method of the composite hydrogel includes the following steps: dissolving fucoidan in an organic solvent, then adding a sulfonating agent to carry out a sulfation reaction; after the reaction, allowing it to stand, precipitate, wash, dry, and grind sequentially to obtain persulfated modified fucoidan; preparing a persulfated modified fucoidan solution, then mixing it evenly with a carboxymethyl chitosan solution, a laver polysaccharide solution, and a hyaluronic acid solution; then adding a polyethylene glycol solution, a 1,4-phenylenediboric acid solution, salmon protein active peptides, and docosahexaenoic acid; finally, adding a crosslinking agent to obtain a mixed solution, and stirring to obtain a composite polysaccharide hydrogel. This composite hydrogel has good mechanical properties and biocompatibility, and can accelerate the healing of chronic wounds in type 2 diabetes.

Provided is an adjuvant for mucosal vaccines comprising a sulfated archaeosome comprising a sulfated glycoarchaeol. The glycoarchaeol may be present as a pharmaceutically acceptable salt. The adjuvant