19 results about "Anticoagulant activity" patented technology

This invention relates to the field of biomedicine, and particularly to a hirudin gene, expression vector, engineered bacteria, recombinant protein, and its applications and production methods, all possessing anticoagulant, thrombolytic, and antioxidant effects. The nucleotide sequence of the hirudin gene is shown in SEQ ID NO: 1. The amino acid sequence of the recombinant protein is shown in SEQ ID NO: 2. The recombinant protein of the hirudin gene provided by this invention, possessing anticoagulant, thrombolytic, and antioxidant effects, exhibits a high concentration of recombinant hirudin, significantly increasing the yield of recombinant hirudin. The recombinant hirudin also demonstrates strong in vitro anticoagulant and thrombolytic activity, indicating broad market prospects.

The invention belongs to the technical field of anticoagulant drugs, and particularly relates to lumbricus protein with anticoagulant activity as well as a preparation method and application of the lumbricus protein. The lumbricus protein provided by the invention contains at least one of a c71616 protein with an amino acid sequence as shown in SEQ ID No. 1, a c128686 protein with an amino acid sequence as shown in SEQ ID No. 2, a c58213 protein with an amino acid sequence as shown in SEQ ID No. 3 and a c118923 protein with an amino acid sequence as shown in SEQ ID No. 4. The protein can effectively inhibit thrombin activity and interfere formation of fibrin so as to act on a key link of a blood coagulation process. The protein can be obtained through recombinant expression and purification, and has high expression efficiency and stability, so that the protein can be used as an active component to be applied to development of anticoagulant drugs, surface anticoagulant coatings and other products.

The preparation method comprises the following specific steps: S1, weighing L-borneol crystal substances, D-borneol crystal substances and Baijiu, and mixing the L-borneol crystal substances, the D-borneol crystal substances and the Baijiu; s2, putting the mixture into a pressure cooker filled with distilled water, heating the interior of the pressure cooker to a boiling state of 100-105 DEG C by using big fire, and continuously cooking for 4-8 hours by using small fire; and S3, carrying out cooling crystallization treatment to obtain the racemic borneol.

This application discloses a complex polysaccharide, its preparation method, and its applications. It discloses both a novel use of sulfated kudzu polysaccharide as a raw material for preparing drugs with anticoagulant effects and a complex polysaccharide comprising sulfated polysaccharide and polysaccharide fragments; wherein the sulfated polysaccharide includes sulfated kudzu polysaccharide and / or sulfated Bletilla striata polysaccharide; and the polysaccharide fragments include glycosaminoglycan fragments. This application modifies the structure of natural polysaccharides to improve their structural properties, preparing and screening complex polysaccharides with anticoagulant activity. The components in the complex polysaccharide work synergistically, significantly enhancing the anticoagulant effect and broadening the application fields of polysaccharides, primarily for the development of anticoagulant drugs and anticoagulant medical devices.

This invention discloses an earthworm protein peptide with in vitro thrombin-inhibiting activity and its preparation method, belonging to the field of functional food technology. The method first pre-treats earthworm raw materials to obtain earthworm protein; then, it uses pepsin and alkaline protease for stepwise enzymatic hydrolysis under varying temperature and pH, and ultrafiltration to collect short peptide solutions with a molecular weight below 3 kDa; the short peptide solution is specifically captured using an affinity chromatography material coupled with bovine thrombin, followed by elution, nanofiltration desalting concentration, and spray drying to obtain the final product. This invention fully releases the peptides through a dual enzymatic hydrolysis process and utilizes thrombin affinity chromatography to directionally enrich peptides with specific inhibitory activity, effectively solving the problems of low content and poor targeting of active ingredients in earthworm protein peptides in existing technologies, and significantly improving the purity and in vitro anticoagulant activity of the product.

The invention discloses a whey protein peptide with high anticoagulant activity and a blood fat reducing effect and a preparation method thereof. The preparation method comprises the following steps: firstly carrying out pulsed electric field pretreatment on a whey protein aqueous solution, then hydrolyzing the pretreated whey protein aqueous solution by using bromelain, and freeze-drying to obtain the whey protein peptide. According to the method, bromelain is selected to carry out enzymolysis on the whey protein aqueous solution, and the whey protein aqueous solution is pretreated by using the pulsed electric field before enzymolysis, so that the use of the pulsed electric field is beneficial to promoting the exposure of peptide chain segments with anticoagulation and blood fat reduction functions, and the anticoagulation and blood fat reduction capabilities of the whey protein peptide are remarkably improved. The method breaks through the limitation of the traditional process, and overcomes the problems of low efficiency and limited active peptide release of a single enzymolysis method through the synergistic effect of the pulsed electric field pretreatment and the enzymolysis technology. The non-thermal effect of the pulsed electric field can directionally change the space structure of the whey protein, more enzyme cutting sites are exposed, and meanwhile protein denaturation caused by high-temperature treatment is avoided.

The present disclosure provides compositions and methods related to nucleic acid molecules having therapeutic aptamers. In particular, the present disclosure provides nucleic acids molecules comprising one or more aptamers having anticoagulant activity, as well as corresponding nucleic acid antidotes, for the modulation of blood coagulation in the context of disease and surgical intervention.

This invention provides a sheep milk casein anticoagulant peptide, its preparation method, and its applications. The sheep milk casein inhibitory peptide is VKETMVPK, VLPVPQK, or EMPFPK. This invention addresses the problems of low safety and high cost of existing clinical anticoagulant drugs, and the low utilization rate of high-value sheep milk casein resources. It fills the technological gap in the preparation of sheep milk casein anticoagulant peptides. The provided sheep milk casein inhibitory peptide has high safety, avoiding the drawbacks of existing drug treatments; efficiently taps the value of sheep milk resources, reducing resource waste; fills a technological gap, establishing a systematic preparation system; has clear and efficient anticoagulant activity with a comprehensive mechanism of action; and has wide applications and great market potential.

The present application relates to the field of living extraction technology, in particular to a leech living body collection and anticoagulant activity substance synchronous extraction process, which comprises the following steps: a large number of living leeches are poured into a shunt box and are shunted by a shunt channel and then fall into a row of a plurality of object cavities; a motor drives a transfer cylinder to rotate intermittently to pick up the living leeches, and the transfer cylinder adsorbs the leeches; when the leeches fall into a plurality of collection cavities of a collection box, the leeches are attracted by a blood-containing artificial membrane and bite, and the saliva is secreted by the current stimulation of an electrode sheet; the saliva flows into a liquid collecting box and is filtered by a filter membrane in the liquid collecting box, the filtered saliva is directly introduced into a low-temperature container through a bottom port connecting pipeline of the liquid collecting box for collection, and the leech anticoagulant is obtained by centrifugal impurity removal. The funnel structure of the shunt box is combined with the flow guide sleeve to increase the single processing capacity, the air pressure adsorption and the intermittent rotation mechanism of the transfer cylinder are used to complete individual transfer, and the electrode sheet and the artificial membrane integrated stimulation system are used to achieve directional saliva collection.

This invention discloses a high-yield, dual-effect earthworm protein small molecule peptide and its preparation method, specifically relating to the field of biomedical technology. The preparation method includes: S1, synergistic enzymatic hydrolysis: earthworm protein undergoes autolysis, followed by enzymatic hydrolysis with a complex enzyme of flavor protease and alkaline protease; S2, charge-size bi-stage membrane separation: the hydrolysate is sequentially passed through a 5kDa first-stage ultrafiltration membrane and a 1kDa second-stage ultrafiltration membrane modified with a positive charge; S3, dual-activity-guided purification: using anticoagulant activity and dipeptidyl peptidase-IV inhibitory activity as tracking indicators, the highly active components are collected. The small molecule peptide obtained by this invention has a molecular weight of 400-800 Da, contains novel sequences shown in SEQ ID NO:1 and SEQ ID NO:2, and simultaneously possesses anticoagulant activity and DPP-IV inhibitory activity, with a yield of 17.2-19.1%, which is more than 30% higher than conventional methods. The process of this invention is stable and has good prospects for industrialization.

The present invention relates to supramolecular agents presenting on demand reversibility of activity and related methods. The invention in particular relates to supramolecular agents with anticoagulant activity which can be reversed with the use of an antidote agent.

A leech extract is a salivary gland extract of poecilobdella manillensis, and is obtained through living body cleaning, gland taking and smashing, purification, separation and multi-step purification. The invention further discloses biological polypeptide composite herbal extract shampoo containing the leech extract and a preparation method of the biological polypeptide composite herbal extract shampoo. The anticoagulant activity of the leech extract is about 1500U, the anticoagulant thrombolytic efficacy is doubled compared with that of a common leech extract, the blood coagulation time can be prolonged, thrombosis can be inhibited, and the fibrinolytic activity can be improved; the effects of improving scalp microcirculation, preventing fibrinogen from forming thrombus, dredging blood capillaries, increasing scalp blood flow, promoting hair follicle nutrition supply and reducing alopecia are achieved.

An anticoagulant phenyl-linked heparin oligosaccharide dimer, a preparation method therefor and use thereof are provided. The anticoagulant phenyl-linked heparin oligosaccharide dimer has a structure as represented by general formula I, and the phenyl may be replaced with substituted phenyl, heteroaryl or substituted heteroaryl. The described phenyl-linked heparin oligosaccharide dimer may be synthesized in fewer steps, has a significantly higher total yield, potent and specific antithrombin-dependent anti-Factor Xa activity without significant anti-FactorIIa activity, the anticoagulant activity may be efficiently neutralized by protamine sulfate, has excellent pharmacokinetic profiles, making it suitable for the preparation of novel anticoagulant and antithrombotic drugs that are cost-effective, higher-quality and safer.

The invention discloses an extraction and separation method of anise polysaccharide with anticoagulant activity. The method comprises the following steps: crushing fresh anise, extracting with hot water, concentrating a water extract, and adding ethanol for precipitation to obtain crude polysaccharide; removing protein by adopting a Sevage method; and dialyzing to remove small molecular impurities, and freeze-drying to obtain the purified anise polysaccharide. The molecular weight of the polysaccharide is about 72.053 kDa. In-vitro coagulation experiments show that the polysaccharide can significantly prolong prothrombin time (PT), activated partial thromboplastin time (APTT) and thrombin time (TT) and reduce the content of fibroblast egg (FIB) white, and shows significant anticoagulant activity. The star anise polysaccharide provided by the invention can be used for preparing anticoagulant drugs, and has the advantages of wide raw material source, simple extraction process, definite activity and the like.

The invention relates to a recombinant hirudin fusion protein, an intelligent response type anticoagulant material as well as a preparation method and application of the intelligent response type anticoagulant material. The intelligent response type anticoagulant material is formed by constructing the recombinant hirudin fusion protein with a carbohydrate binding domain (CBM) and combining the recombinant hirudin fusion protein with bacterial nanocellulose or a composite material of the bacterial nanocellulose. The recombinant hirudin fusion protein disclosed by the invention can specifically respond to blood coagulation formation related enzymes so as to recover the anticoagulant activity, and can be well combined with a bacterial nanocellulose material or a composite material thereof; the intelligent response type anticoagulant material is mild in preparation condition and green and environment-friendly in technology, and has great potential in the medical application aspect of membranous anticoagulant biological materials such as heart valves and the like and tubular anticoagulant biological materials such as artificial blood vessels and the like.

The invention discloses an extraction and separation method of atractylodes macrocephala polysaccharide with anticoagulant activity, which comprises the following steps: by taking atractylodes macrocephala rhizome as a raw material, performing ethanol degreasing, hot water extraction and ethanol precipitation to obtain crude polysaccharide, then performing Sevage method deproteinization and dialysis purification, and finally performing ethanol fractional precipitation to obtain a plurality of polysaccharide components. Wherein the 80% ethanol precipitation component (AMP-80) has optimal homogeneity and concentrated molecular weight distribution, the total sugar content is 92.18%, the uronic acid content is 24.65%, and the main monosaccharide composition is glucose (45.13%), galacturonic acid (25.46%) and arabinose (21.60%). In-vitro coagulation experiments show that the AMP-80 can significantly prolong PT, APTT and TT in a concentration range of 2.5-10 mg / mL, reduce the content of FIB and show good anticoagulant activity. The atractylodes macrocephala polysaccharide component provided by the invention can be used for preparing anticoagulant drugs, and has the advantages of easily available raw materials, controllable process, definite activity and the like.

This invention discloses a fibrinolytic enzyme-producing Pseudomonas hygroscopicus originating from mangrove wetlands (… Fictibacillus rigui The strain QMS71 is deposited at the China General Microbiological Culture Collection Center (CGMCC) under accession number CGMCC No. 37018. Under suitable culture conditions, strain QMS71 can produce extracellular enzymes with fibrin-degrading activity. Its fermentation products exhibit significant fibrin degradation activity in vitro and demonstrate thrombolytic and anticoagulant activities in relevant in vitro functional evaluation models. This invention provides a well-identified fibrinolytic enzyme-producing microbial resource and its applications, which can be used for in vitro functional evaluation, model studies, and functional enzymology research related to fibrin degradation.

The invention belongs to the technical field of biological medicine, and particularly relates to earthworm anticoagulant oligopeptide and application thereof. The invention relates to an anticoagulant oligopeptide, which is found by combining the activity-directed separation of pheretima asper (E. Perriver) with the screening of an AI (artificial intelligence) prediction model. The amino acid sequence of the oligopeptide is LMWFSDRNK; the oligopeptide regulates thrombin in an allosteric manner, enhances the combination of thrombin and antithrombin, and can effectively inhibit blood coagulation and thrombus formation at in-vivo and in-vitro levels; the oligopeptide has a remarkable anticoagulant effect and is suitable for research and development of antithrombotic drugs.

The invention provides a sheep milk casein anticoagulant peptide as well as a preparation method and application thereof. The sheep milk casein anticoagulant peptide is VKETMVPK, VLPVPQK or EMPFPK. The invention solves the problems of low safety and high cost of the existing clinical anticoagulant drug; the high-valued utilization rate of sheep milk casein resource development is low; and the blank of the preparation technology of the sheep milk casein anticoagulant peptide is filled. The sheep milk casein inhibitory peptide provided by the invention has high safety, and avoids the defects of existing drug treatment; goat milk resource value is efficiently excavated, and resource waste is reduced; the technical blank is filled, and a systematic preparation system is established; the anticoagulant activity is clear and efficient, and the action mechanism is comprehensive; the application scene is wide and the market potential is great.