55 results about "Anticoagulant effect" patented technology

The invention relates to an anticoagulant medical device and a preparation method thereof. The preparation method of the anticoagulant medical device comprises the following steps: covalently connecting the hydrophilic anticoagulant layer on the surface of the base material in an atom transfer radical polymerization manner; a heparin anticoagulation layer is covalently connected to the surface of a hydrophilic anticoagulation layer to prepare the anticoagulation medical device, and the heparin anticoagulation layer and the hydrophilic anticoagulation layer are connected through one or more covalent bonds of a triazole bond, an amido bond, a carbon-nitrogen single bond and a carbon-sulfur single bond. According to the preparation method of the anticoagulation medical device, the anti-protein adsorption effect of the hydrophilic anticoagulation layer and the anticoagulation effect of the heparin anticoagulation layer can be synergistically exerted, the anticoagulation effect is improved, and meanwhile, the anticoagulation stability is improved.

This invention discloses an arabinogalactan sulfate derived from *Streptococcus filamentosa*, its preparation method, and its applications, belonging to the fields of natural product chemistry and marine biological resource development technology. The technical solution includes an arabinogalactan sulfate derived from *Streptococcus filamentosa*, with a monosaccharide composition comprising arabinose, galactose, and rhamnose, the molar percentage of arabinose being not less than 70%; the main chain backbone is modified with natural sulfate groups, with the sulfate groups replacing at least one hydroxyl site of the arabinose residues. This invention, when applied to anticoagulation, exhibits good safety and a significant anticoagulant effect.

The invention relates to the technical field of medical materials, and particularly discloses a heparinized polycaprolactone coating as well as a preparation method and application thereof. The preparation method of the heparinized polycaprolactone coating comprises the following steps: adding poloxamer 188 into an MES buffer solution, adding heparin, uniformly stirring, then adding EDC and NHS, and stirring for 40-120 minutes to prepare an activated heparin solution; and dropwise adding the polycaprolactone solution into the activated heparin solution, reacting at room temperature for 90-180 minutes, then dissolving the conjugate into chloroform through rotary evaporation, washing with water, drying with anhydrous MgSO4, precipitating with methanol, filtering the precipitate, and drying in vacuum at 30-40 DEG C to obtain the polycaprolactone heparin. According to the prepared heparinized polycaprolactone coating, local release of heparin is remarkably reduced, and the continuous anticoagulation effect is achieved; and the anticoagulant activity of heparin is reserved, and the coating with good stability is obtained.

The invention discloses a traditional Chinese medicine composition for treating ischemic diseases of lower limbs and also provides a preparation method of the traditional Chinese medicine composition, the synergistic combination effect of the two medicines is exerted, the curative effect of a single medicine is obviously improved, and the anticoagulant effect is far greater than that of the independent administration of caltrop; the sum of the action intensity of two independent administration is achieved, and the in-vitro anticoagulation effect and the effect of improving the ischemic blood flow of the lower limbs can be remarkably improved; blood vessel growth factors are remarkably increased, the activity of creatine kinase and lactic dehydrogenase is reduced, and tissue damage is relieved; glutathione peroxidase is promoted, malondialdehyde and active oxygen are inhibited, and the anti-oxidation effect is achieved; hypoxia and mitochondrial functions can be improved by inhibiting hypoxia-inducible factors (hypoxia-inducible factors)-1 alpha and mitochondrial compounds 3; the anti-inflammatory effect can be achieved by inhibiting CRP, IL-1beta and TNF-alpha; the rat lower limb ischemia can be obviously improved and treated.

The application provides a use of a combination of metoclopramide and latanoprost for preparing an anticoagulant drug, and belongs to the technical field of biological medicines. It is accidentally found that the anticoagulant effect of patients with deep vein thrombosis combined with high intraocular pressure is improved after long-term continuous oral administration of metoclopramide. Thanks to this beneficial discovery, the application realizes good anticoagulant effect by applying the latanoprost liniment in a topical manner on the basis of oral administration of metoclopramide. The application is convenient to administer, has definite and sustained curative effect, and is safe and reliable. The application expands the optional range of anticoagulant drugs, and realizes better curative effect at the same time, and has outstanding technical advantages and wide popularization prospect.

This invention relates to the field of biomedicine, specifically to the application of banana fruit extract in the preparation of anticoagulant or antithrombotic drugs. The banana fruit extract provided by this invention contains flavonoids, polyphenols, polysaccharides, hydroxyanthraquinones, triterpenoids, and other components. Flavonoids and polyphenols have been widely proven to have anti-inflammatory and antioxidant effects, which can synergistically enhance the anticoagulant effect. The high potassium content of banana fruit can indirectly improve vascular function by regulating sodium-potassium balance. The banana fruit extract provided by this invention can inhibit platelet production or promote platelet metabolism, prolong clotting time, and inhibit platelet aggregation. Its natural plant components interfere with platelet aggregation by inhibiting the production of thromboxane A2, achieving anticoagulant and antithrombotic effects. The mechanism of action of banana fruit extract differs from traditional anticoagulants such as heparin, reducing the risk of bleeding. Its natural component characteristics provide a direction for the development of novel plant-derived anticoagulant drugs.

The invention discloses a blood sampler, and relates to the technical field of blood sampling. Comprising a blood collection piece, a flow guide piece is installed in the blood collection piece, and the flow guide piece is used for guiding blood to the bottom of the blood collection piece; the blood collection piece is sleeved with a lifting adjusting piece; a lifting isolation piece is mounted in the blood collection piece; the lifting isolation piece magnetically attracts the lifting adjusting piece; a puncture sampling piece is mounted on the blood collection piece; an anticoagulant is conveniently drained to the inner side of the drainage tube by utilizing the lifting isolation piece, and the flow guide piece can be used for draining blood, so that the blood can be conveniently and directly discharged from an anticoagulant solvent at the bottom of the blood collection tube during collection, and the anticoagulant effect is ensured; the problems that according to an existing blood sampler, direct anticoagulation drainage to a solvent is not convenient, clear liquid is not convenient to isolate, protect and separate, and butt joint is tedious through manual single-hand operation are solved.

The present invention relates to a cancer-targeting drug delivery system comprising: a protamine-based self-assembling first nanoparticle including a negatively charged substance; and a heparin-based self-assembling second nanoparticle including a positively charged anticancer agent and Fe3+. Specifically, the present invention provides a drug delivery system that forms aggregates at a tumor site by a guidance effect upon administration of the second nanoparticle after administration of the first nanoparticle, and has an anticancer effect through induction of ferroptosis and immunogenic cell death without exhibiting the anticoagulant effect of heparin.

A transdermal patch for delivering lidocaine for pain relief comprises a backing layer, an adhesive layer, and a composition containing lidocaine in an amount ranging from about 1% to about 10% by weight and heparin as a non-anticoagulant permeation enhancer in an amount ranging from about 1% to about 50% by weight. The heparin enhances permeation of lidocaine through the skin without exerting systemic anticoagulant effects by temporarily disrupting lipid bilayers in the stratum corneum to create microchannels for increased diffusion of lidocaine molecules. The patch provides effective relief for multiple pain types including neuropathic pain, inflammatory pain, ischemic pain, post-surgical pain, nociplastic pain, mechanical pain, and compressive pain. The transdermal patch is configured to provide sustained release of lidocaine for periods ranging from about 8 hours to about 24 hours, reducing pain by at least 50% with minimal systemic absorption.

The invention relates to the technical field of anticoagulant coatings, and discloses an ePTFE membrane grafted with a heparin and aminated hyaluronic acid coating and a preparation method. According to the method for preparing the ePTFE membrane grafted with the heparin and aminated hyaluronic acid coating, a primer layer is formed on the surface of the ePTFE membrane in a plasma polymerization mode, and heparin and hyaluronic acid are chemically and covalently grafted on the primer layer, so that the surface of the membrane has long-term stability and an anticoagulation effect; the problems that in the prior art, reaction is complex, solvent residues easily exist, stability is poor, the anticoagulant effect is poor, and gaps are blocked are solved.

The present invention discloses a coating material for an injection needle and its use in the surface treatment of a peripheral blood collection needle, and relates to the field of biomedical materials technology. The coating material for an injection needle is formed by dipping the injection needle into a coating solution to form a coating on the needle surface; wherein the raw material composition of the coating solution includes, by weight, 1 to 4 parts by weight of modified silicone oil, 20 to 30 parts of 4-chlorotrifluorotoluene, 15 to 30 parts of ethyl acetate, 2 to 4 parts of hydroxypropyl methylcellulose, 3 to 8 parts of dimethylformamide, 0.5 to 2 parts of fucoidan sulfate, and 0.1 to 1 part of luteolin-5-O-glucoside. The coating material prepared by the present invention has good adhesion, is not easy to fall off when applied to the needle surface, and can greatly reduce the pain of the patient during puncture; has excellent anticoagulant effect, and can alleviate the problem that the patient's blood coagulation is fast and is not conducive to subsequent testing.

The present application relates to the technical field of cardiac surgery, and in particular to a drainage device with pericardial positioning and anticoagulant efficacy after cardiac surgery. The drainage device with pericardial positioning and anticoagulant efficacy after cardiac surgery comprises a tube body, a positioning portion, and a drug supply portion; one end of the tube body is an insertion end for placement into the pericardium, a drug supply channel and a diversion channel for pericardial liquid to pass through are formed in the tube body, the diversion channel and the drug supply channel are isolated from each other, and the drug supply portion is connected to the drug supply channel so that the drug supply portion can drive the drug into the drug supply channel; the diversion channel forms a diversion port at the insertion end, the drug supply channel forms a drug supply port at the insertion end, the positioning portion is installed on the drug supply port, the positioning portion is used to cooperate with the blood clot in the pericardium to limit the position, and a delivery channel connected to the drug supply channel is formed in the positioning portion. The diversion channel and the drug supply channel are integrated in the tube body, and there is no need to place multiple tube bodies in the pericardium, which reduces the multiple traumas of placing the drainage device into the pericardium, reduces the difficulty of drug administration, and improves the accuracy of drug administration.

The invention discloses a preparation method of Panax stipuleanatus protein, which comprises the following steps: adding 0.01 mol / L NaOH solution with pH of 9 into Panax stipuleanatus powder, uniformly mixing, centrifuging at 4 DEG C, and taking filter residue; adding an ethanol solution with the volume concentration of 70-80% into the filter residues, extracting and centrifuging at 4 DEG C to take the filter residues, adding a NaCl solution with the mass concentration of 2-4% into the filter residues, extracting and centrifuging at 4 DEG C, taking the filter residues again, adding water into the filter residues, extracting and centrifuging at 4 DEG C, and taking supernate; the supernatant is dialyzed for 12-24 h at the temperature of 4 DEG C with a dialysis bag with the molecular weight cut-off of 10 kDa, inner filtrate is collected, freeze drying is conducted, then the panax stipuleanatus protein is prepared, anticoagulant activity detection is conducted on the panax stipuleanatus protein, and the protein has the good anticoagulant effect; the preparation method disclosed by the invention is simple to operate, provides a new way for preparing a novel anticoagulant drug, and has a wide application prospect.

The invention relates to a heparin anticoagulant pentasaccharide capable of neutralizing and adjusting half-life period as well as a preparation method and application of the heparin anticoagulant pentasaccharide. Comprising the following steps: (1) preparing heparin hexasaccharide; (2) preparing heparin anticoagulant hexasaccharide; and (3) preparing the heparin anticoagulant pentasaccharide capable of neutralizing and adjusting the half-life period. The heparin anticoagulant pentasaccharide provided by the invention is structurally similar to the unique ultra-low molecular weight heparin fondaparinux sodium in the current market, a biotin group is only introduced to an E glycosyl group at a non-reducing end, so that rapid neutralization can be effectively realized through avidin, and by introducing PEG (Polyethylene Glycol) connexons with different lengths, the heparin anticoagulant pentasaccharide can be rapidly neutralized. The prepared heparin anticoagulant pentasaccharide can adjust the half-life period of ultra-low molecular weight heparin. Therefore, the lasting time of the medicine effect of the ultra-low molecular weight heparin is prolonged, and the requirement of frequent administration is reduced. Therefore, the anticoagulant effect of the ultra-low molecular weight heparin is more controllable, the treatment risk is reduced, and the treatment effect is improved.

The invention discloses an anticoagulant active site of massa medicata fermentata fujianensis as well as a preparation method and application thereof, and belongs to the field of biological medicines. The massa medicata fermentata fujianensis is used for preparing anticoagulant drugs for the first time. The method comprises the following steps: soaking a fermented mass raw material with methanol, performing reflux extraction, and recovering a solvent under reduced pressure to obtain a total extract; and separating the total extract by adopting macroporous adsorption resin column chromatography to obtain a water part, a 30% methanol part, a 50% methanol part and a pure methanol part. Platelet aggregation detection results show that all the parts can inhibit ADP-induced platelet aggregation, and the anticoagulant effect of the 30% methanol part is most prominent and is similar to that of a positive drug. According to the preparation method of the anticoagulant active part of the massa medicata fermentata fujianensis, the anticoagulant active part in the massa medicata fermentata fujianensis can be effectively separated and enriched, and the obtained anticoagulant active part can be applied to preparation of anticoagulant drugs and has good industrial application prospects and clinical value.

The present invention relates to the technical field of medicine, and to derivatives represented by formula 1 and formula 2 in which a carboxylic acid group is introduced into the structure of Ginkgolide B by means of a hydroxyl group at the 10-position and ester derivatives of carboxylic acid groups, and pharmaceutically acceptable organic or inorganic salts. Ginkgolide B is used as a parent body and is prepared by means of chemical structure modification so as to achieve the goals of improving solubility, increasing bioavailability and enhancing healing efficacy. The prepared compound and carboxylate salts thereof have significant platelet activating factor antagonism, an anticoagulant effect and an anti-acute cerebral ischemia effect, and can be used for preparing a drug for preventing and treating ischemic stroke, thrombosis, angina pectoris, cardiopulmonary infarction, as well as inflammation, asthma and other diseases related to a platelet activating factor.

This invention provides a gene encoding a coral-derived anticoagulant polypeptide and its applications, belonging to the field of bioactive peptide technology. The invention provides an anticoagulant polypeptide GcKuz1, which includes the mature peptide segment shown in SEQ ID No. 1. The polypeptide GcKuz1 of this invention exerts antithrombotic and anticoagulant effects by binding to KLKB1 in the thrombin system. This polypeptide does not cause hemolysis or cytotoxicity, and has no bleeding risk, exhibiting good biocompatibility. The anticoagulant polypeptide of this invention has therapeutic effects on thrombotic diseases, such as arterial thrombotic diseases, venous thrombotic diseases, and capillary thrombotic diseases.

A hollow multilayer MOF material and its preparation method and application relates to the field of nano drug delivery therapy technology. A ZIF-67@ZIF-8@ZIF-67@ZIF-8 hollow multilayer MOF material is applied as a thrombus drug nanocarrier to prepare drugs for thrombus treatment. In vitro experiments indicate that the thrombus drug nanocarrier has an efficient targeting effect and an excellent sustained release effect in combination with streptokinase and warfarin, and the thrombus drug nanocarrier has a good therapeutic effect and the long-term anticoagulant effect.

The invention discloses a base material coated with an anticoagulant coating and a preparation method of the base material. The preparation method comprises the following steps: immersing a base material into a polyhydroxy polymer solution, and carrying out hydroxylation modification on the surface of the base material to prepare a base material 1; immersing the base material 1 into the multi-amino polymer solution, and fully reacting to prepare a base material 2; immersing the base material 2 into a polymer solution containing a coupling agent and carboxyl and / or sodium carboxylate groups, and fully reacting to prepare a base material 3; immersing a base material 3 into the multi-amino polymer solution, and fully reacting to prepare a base material 4; and immersing the base material 4 into a heparin sodium solution, and fully reacting to prepare the heparin-grafted anticoagulant coating. The prepared anticoagulant coating is good in uniformity, high in coverage degree, firm and lasting in anticoagulant effect, and the heparin grafting content on the surface of the material can be regulated and controlled according to actual clinical requirements.

The invention discloses an antibacterial and anticoagulant bioactive coating and a preparation method thereof, and relates to the technical field of biomedical materials. Comprising the following steps: step 1, adding chitosan into a solvent, and uniformly stirring to obtain a chitosan solution; adding a copper ion source, stirring and chelating to obtain a chitosan chelated copper ion compound solution; 2, tannic acid is added into the chitosan chelated copper ion compound solution, stirring and mixing are conducted, and a mixed solution is obtained; 3, a base material is immersed in the mixed solution, the pH of the mixed solution is adjusted to be alkaline, constant-temperature standing reaction is conducted, washing and drying are conducted, and a finished product with the bioactive coating is obtained. The medical instrument prepared by the method has good antibacterial and anticoagulant effects.

The present invention provides a leech-derived polypeptide having both analgesic and antithrombotic functions, a precursor protein thereof, and applications thereof, belonging to the technical field of functional peptides. The leech-derived polypeptide provided by the present invention has an amino acid sequence as shown in SEQ ID NO: 1. The leech-derived polypeptide provided by the present invention can inhibit the activity of proteases such as elastase, FXIIa, and kallikrein, and has good anticoagulant effects, while also having good analgesic and antithrombotic effects. The polypeptide has the characteristics of a simple structure, strong analgesic and antithrombotic activities, and can be used as an analgesic and antithrombotic drug.

The application discloses an anti-heparin-antithrombin complex antibody and application thereof in a heparin drug anticoagulation effect detection method, and comprises an antibody molecule capable of combining with a heparin-antithrombin complex, a light chain variable region amino acid sequence of the antibody molecule is shown as SEQ ID NO:1, and a heavy chain variable region amino acid sequence is shown as SEQ ID NO:2. The antibody molecule can be prepared into a kit for detecting the anticoagulation effect of the heparin drug, and has the characteristics of simple operation, high sensitivity, good repeatability, wide linearity and the like, and is suitable for high-throughput testing of the anticoagulation effect of the heparin drug.

The invention belongs to the field of functional medical catheter preparation, and particularly relates to an antibacterial and anticoagulant dialysis catheter with a sodium alginate-graphene oxide coating and a preparation method of the antibacterial and anticoagulant dialysis catheter. The preparation method comprises the following steps: (1) mixing a graphene oxide dispersion liquid with a sodium alginate solution, then adding a water / alcohol mixed solvent, and then carrying out ultrasonic dispersion treatment to obtain a mixed solution; (2) performing plasma pretreatment on the polyurethane dialysis catheter to obtain a pretreated catheter; (3) immersing the pretreated conduit into the mixed solution, and after dip-coating is completed, sequentially standing and drying to obtain a primary gel layer conduit; and (4) immersing the primary gel layer catheter into a cross-linking solution, and then sequentially cleaning, gradient heating and curing. Efficient antibiosis and anticoagulation are achieved through the synergistic effect of materials, the coating is firm in combination and excellent in biocompatibility, the problems of thrombus formation, infection and the like of an existing dialysis catheter can be solved, and the coating is suitable for hemodialysis vascular access.

This application discloses a complex polysaccharide, its preparation method, and its applications. It discloses both a novel use of sulfated kudzu polysaccharide as a raw material for preparing drugs with anticoagulant effects and a complex polysaccharide comprising sulfated polysaccharide and polysaccharide fragments; wherein the sulfated polysaccharide includes sulfated kudzu polysaccharide and / or sulfated Bletilla striata polysaccharide; and the polysaccharide fragments include glycosaminoglycan fragments. This application modifies the structure of natural polysaccharides to improve their structural properties, preparing and screening complex polysaccharides with anticoagulant activity. The components in the complex polysaccharide work synergistically, significantly enhancing the anticoagulant effect and broadening the application fields of polysaccharides, primarily for the development of anticoagulant drugs and anticoagulant medical devices.

The invention discloses a polyurethane solid microsphere with an anticoagulant effect, a preparation method and application thereof, and belongs to the technical field of blood purification. The preparation method comprises the following steps: adding a polyurethane synthetic monomer and an initiator to a solvent to initiate a polymerization reaction; subsequently, adding glycerol and polyethersulfone to the reaction solution for micro-crosslinking and blending; cooling the solution after the reaction is completed, and then obtaining polyurethane anticoagulant microspheres in a coagulation bath through phase inversion; finally, removing impurities and deprotonating the microspheres; the invention also discloses an application of the microspheres obtained by the above method; the polyurethane microspheres of the invention can achieve anticoagulation by reducing the levels of blood calcium ions and coagulation factors, while not affecting the recovery of the coagulation ability in the patient's body.

The utility model relates to the technical field of blood collection, in particular to a blood collection anti-coagulation device which comprises an outer frame body, an inner frame body, a working frame body and an anti-coagulation structure, the inner frame body is installed in the outer frame body in a sliding mode, the working frame body is installed in the inner frame body in a sliding mode, and the anti-coagulation structure comprises a driving body, a horizontal anti-coagulation assembly and a longitudinal anti-coagulation assembly. A driving body is installed at the bottom in the outer frame body, a horizontal anti-condensation assembly and a longitudinal anti-condensation assembly are sequentially and fixedly installed on the surface of the driving body, and the driving body synchronously drives the horizontal anti-condensation assembly and the longitudinal anti-condensation assembly to work, so that the inner frame body is driven to horizontally slide in a reciprocating mode, and the working frame body is driven to longitudinally horizontally move in a reciprocating mode; therefore, the anticoagulant can be uniformly distributed through longitudinal and transverse oscillation, the mixing effect of the anticoagulant and blood is improved, and the problem that the anticoagulant effect is affected due to the fact that the blood and the anticoagulant are easily mixed unevenly is solved.

The invention belongs to biomedical materials, and particularly relates to an antibacterial and anticoagulant coating material for a blood contact material and application of the antibacterial and anticoagulant coating material. The antibacterial and anticoagulant coating material comprises copper tetracarboxyl phenyl porphyrin loaded with an anticoagulant and water-soluble polysaccharide. A coating formed on the basis of the antibacterial and anticoagulant coating material can achieve antibacterial and anticoagulant effects at the same time, for example, the bacteriostasis rate on escherichia coli and the bacteriostasis rate on staphylococcus epidermidis can reach 90%; in addition, the activated part thromboplastin time, prothrombin time and thrombin time of the coating are obviously higher than those of an untreated blood contact material; and the hemolysis rate is lower than 5%.

The present invention belongs to the technical field of heparin anticoagulant coatings, and in particular to a long-acting heparin anticoagulant coating and a preparation method thereof. The long-acting heparin anticoagulant coating is formed by curing the long-acting heparin anticoagulant coating, which includes component A and component B; the raw materials of component A include at least one of a monomer, an initiator, a polymer, poly-L-lysine hydrobromide, streptavidin, sodium hyaluronate, and crotonaldehyde; wherein the monomer includes at least one of MPC, PEGMA, and SBMA, and the polymer includes an amino substrate; the raw materials of component B include at least one of a reducing agent and heparin. Preparation method: the long-acting heparin anticoagulant coating is circulated in the ECMO system and cured to obtain a long-acting heparin anticoagulant coating. The long-acting heparin anticoagulant coating constructed in the present application can achieve a long-lasting and stable anticoagulant effect in the ECMO system, and can solve the defects of the prior art.

The invention provides a rare earth anticoagulant material for a venous pipeline and a preparation method and application thereof.The rare earth anticoagulant material is prepared from raw materials including ferulic acid and rare earth nitrate, the ferulic acid is a phenolic acid substance with the anti-oxidation, anti-inflammation and antibacterial effects, the chemical property of rare earth ions is quite similar to that of calcium ions, and the rare earth nitrate has the anti-oxidation, anti-inflammation and antibacterial effects. The rare earth ions are more than calcium ions by one positive charge, the ion charge density is higher than that of the calcium ions, and the rare earth ions have stronger combining capacity with oxygen-containing ligands and can competitively replace calcium to form a more stable complex, so that the protein structure is influenced, the normal blood coagulation process is destroyed, and the anticoagulation effect is achieved; the combination of the two can obtain a material with a better anticoagulant effect.

The invention discloses a nano drug delivery system and a preparation method and application thereof.The nano drug delivery system comprises nano particles, a platelet membrane, a tissue-type plasminogen activator and a thrombin specific aptamer, the nano particles are at least partially coated with the platelet membrane, and the thrombin specific aptamer is at least partially coated with the tissue-type plasminogen activator. The tissue-type plasminogen activator and the thrombin specific aptamer are connected to the platelet membrane. The nano drug delivery system disclosed by the invention has good targeting property, a rapid thrombolysis effect and a local anticoagulation effect, and is beneficial to avoiding secondary coagulation and reembolism risks.