The invention discloses a preparation method of a sulfonated polypropylene non-woven fabric anticoagulant material, which belongs to the technical field of anticoagulant material preparation. The present invention uses konjac powder as a raw material, which is dispersed and extracted by ultrasonic waves, then precipitated with absolute ethanol, centrifuged, and the filtrate is ultrafiltered, subjected to secondary alcohol precipitation, combined and precipitated, vacuum freeze-dried, and then reacted with sulfuric acid esterification react with reagents, adjust the pH to obtain sulfated konjac glucomannan, and then soak the polypropylene non-woven fabric that has been irradiated and grafted in the sulfated konjac glucomannan solution to obtain sulfonated polypropylene non-woven fabrics. Spun anticoagulant material, the anticoagulant material of the present invention has good compatibility with blood, the life of anticoagulant is prolonged, the storage time of blood can be extended by 2-4 weeks, the incidence of thrombus is reduced by 40-60%, and there is Good application prospects.