112results about How to "High anticoagulant activity" patented technology

The invention provides a derivate of low molecular weight fucosylated glycosaminoglycan (dLFG) and a medical composition containing the dLFG or pharmaceutically acceptable salt and application of the dLFG and the medical composition for preparing medicines for treating thrombotic diseases.

The invention relates to a de-polymerizing sea cucumber glycosamino-glycan compound, comprising 90-99. 89% of de-polymerizing sea cucumber glycosamino-glycan with weight average molecular weight being between 8000Da to 120000Da. The preparation method comprises following steps: (1) extracting sea cucumber polysaccharides from sea cucumber; (2) degrading extracted polysaccharides; (3) collecting de-polymerizing sea cucumber glycosamino-glycan with weight average molecular weight being between 8000Da to 120000Da. The compound can prevent and treat cerebrovascular disease and cardiovascular disease, especially blood coagulation disease and thrombus disease. The raw material is sea creature, so the source is abundant and cost is low.

The invention discloses a preparation method of a hyperbranched polyimide anti-coagulant antibacterial material, and belongs to the field of chemical materials. The surface of amino-terminated hyperbranched polyimide is subjected to graft modification by 2-methacryloyloxyethyl phosphorylcholine to form a hyperbranched polyimide film, and the biocompatibility, anticoagulant property and antibacterial property of the prepared HBPI-MPC film are good.

The invention relates to a medical product for interventional therapy, in particular to a novel intravascular coated stent which comprises a stent body and a coat. The coat comprises a polymer bottom layer formed by coating on the surface of the stent body and multilayer silk peptide layers and chitosan layers which are formed by coating on the surface of the polymer bottom layer, wherein the silk peptide layers and the chitosan layers are arranged alternately. A stent coat is prepared by a method of layer upon layer electrostatic self-assembly, has better compatibility with blood, and can reduce late restenosis rate of stent post-operative heart.

The invention discloses an anticoagulation coating material and a preparation method thereof. The anticoagulation coating material comprises sodium hyaluronate and hirudin, wherein the sodium hyaluronate is formed into a gel network; the hirudin is in covalent binding with the sodium hyaluronate. The preparation method of the anticoagulation coating material comprises the following steps: (1) preparing an MES (Fatty Acid Methyl Ester Sulfonate) buffer solution; (2) preparing a sodium hyaluronate solution with the concentration of 1mg / ml to 20mg / ml; (3) preparing a hirudin solution with the concentration of 1mg / ml to 10mg / ml; (4) uniformly mixing up the two solutions so as to enable the mass ratio of the hirudin to the sodium hyaluronate to be 1:1 to 1:10; (5) adding a crosslinking agent into the mixture solution obtained in the step (4), uniformly mixing up so as to covalently crosslink the hirudin and the sodium hyaluronate. The anticoagulation coating material disclosed by the invention is high in anticoagulation activity, stable in effect, long in lasting time, good in biocompatibility, simple in preparation method, gentle in condition and applicable to large-scale industrial production.

The invention discloses a preparation method of a hyperbranched polyimide anticoagulant and antibacterial material, which belongs to the field of chemical materials. The present invention uses 2-methacryloyloxyethyl phosphorylcholine to graft and modify the surface of amino-terminated hyperbranched polyimide to form a hyperbranched polyimide film, and the prepared HBPI-MPC film biophase Capacitive, anticoagulant and antibacterial properties are better.

The invention provides a pharmaceutical composition containing active procyanidins and prasugrel or the pharmacologically acceptable salt thereof. The procyanidins and the prasugrel are used in a combined manner, a more effective method for curing thrombotic diseases is found, after trail for many times, the prasugrel and the procyanidins (an extract of traditional Chinese medicine) are creatively and effectively combined together, the effect of applying the procyanidins to inhibit the thrombosis in a combined manner is discovered unexpected in the process of applying the prasugrel to cure the thrombotic diseases, and not only the anticoagulant effect of the prasugrel is free from the influence of the procyanidins, but also a good effect is achieved in the aspect of reducing the adverse reaction of bleeding after the use of the prasugrel and the procyanidins in a combined manner, therefore, the risk of bleeding is greatly reduced when the advantages of good anticoagulant activity and fast effect of the prasugrel during the antiplatelet aggregation are fully exerted, the risk of bleeding of the prasugrel during the antiplatelet aggregation is effectively reduced and the adverse reaction of the prasugrel is greatly reduced.

The present invention discloses one kind of new compound sodium alginate sulfate and its preparation. The preparation process includes adding ad alginate into sulfatizing reagent, reaction at 40-70 deg.c to obtain reddish brown solution, dialysis and concentration to obtain sodium alginate sulfate. The in vitro blood coagulation with the obtained sodium alginate sulfate shows that it has very high anticoagulant activity and has APTT value reaching the level of haparin in the said concentration, so that sodium alginate sulfate may be used as anticoagulant in preparing blood coagulation resisting medicine.

The invention discloses a method for preparing heparinoid polysaccharide. The heparinoid polysaccharide is prepared by chitin through phosphate treatment, a 6-bit selective oxidation reaction, deacetylation reaction, sulfonation reaction and / or a sulfation reaction, the substitution degree of sulfate groups thereof is between 0.3 and 1.35, and the molecular weight is between 0.40*104 and 1.5*104. The method has good repeatability; and the prepared heparinoid polysaccharide has a structure close to that of heparin, has higher anticoagulant activity, and expects to substitute the heparin to serve as an anticoagulant medicament.

The invention discloses an anticoagulant polypeptide, belonging to the field of biomedicine. The primary structure of the anticoagulant polypeptide is shown as the general formula: (D)-FPRP-X1-X2-X3-X4-X5-QGDFEPIPEDAYDE-NH2. The invention has the advantages that the invention designs and synthesizes one type of anticoagulant polypeptide according to the structure characteristics and action mode of thrombin and Bivalirudin which is anticoagulant drug, and the polypeptide can inhibit the activity of the thrombin efficiently and specifically; furthermore, the number of amino acid in the anticoagulant polypeptide is equal to that of the Bivalirudin, thus synthesis is easy, and the activity of anticoagulation is stronger.

A thrombus target fusion mA5UKB with both anticoagulating and thrombolytic functions is prepared from the mutant mAnxA5 of AnnexinA5 having anti-coagulating function and the urokinase B chain (UKB) having thrombolytic function through fusing. It can be used to prepare the thrombolytic medicine.

The invention relates to polypeptides with anticoagulation activity and a method for screening polypeptides with anticoagulation activity by a phage display technique, belonging to the technical field of development, research and application of anticoagulant drugs. The screening method comprises the following steps: construction of protein expression vector, expression and purification of target protein, verification of target protein, elutriation of bioactive peptide capable of specifically binding target protein by phage display, polypeptide anticoagulation action in-vivo / in-vitro detection, toxicity experimentation and the like, thereby finally obtaining the polypeptides with anticoagulation activity. The polypeptides are prepared by the following steps: by using a heparin-binding epidermal growth factor as a target molecule, carrying out elutriation three times by using a phage display technique, and eluting the specific binding target molecule phage by using heparin sodium as an effective component. The polypeptides can be used for preparing anticoagulant drugs. The polypeptides have shorter sequence, are easy for synthesis, can easily implement large-scale production, and have no obvious short-term and long-term toxicity for mice in vivo, thereby having important application value in the aspect of development and research of anticoagulant drugs.

The invention relates to a preparation method for a medical product for interventional therapy, in particular to a preparation method for a novel intravascular coated stent. The preparation method comprises the following steps: coating on the surface of a stent body through a method of electrostatic self-assembly layer upon layer to form a polymer bottom layer, and alternately coating on the surface of the polymer bottom layer to form multilayer silk peptide layers and chitosan layers. A stent coat obtained by the preparation method has better compatibility with blood, and can reduce late restenosis rate of post-operative heart.

The invention relates to biomedicines, which is a drug used for the treatment and prevention of thrombosis diseases, in particular to an application of sulfated polysaccharide in the preparation of anti-prothrombin activity drugs. The invention has the advantages that: the inulin sources are rich, the preparation conditions of sulfated inulin derivatives are easy to operate, and the invention is applicable to large-scale production; the anti-prothrombin effect is significant, the development prospect is broad and the significance is far-reaching.

The invention discloses applications of a peptides compound in rhizome sparganii in preparing anticoagulant and / or antithrombosis medicaments. The structural formula of the peptides compound is shown in the formula (I). The peptides compound obtained by separating and purifying rhizome sparganii, and applications thereof are provided for the first time, the compound plays a role in prolonging the prothrombin time (PT), activated partial thromboplastin time (APTT) and thrombin time (TT), has good anticoagulation activity, and provides a powerful basis for developing natural antithrombosis medicaments. The formula (I) is as shown in the specification.

The invention relates to a preparation method of multipurpose functional water. The preparation method comprises the following steps of: preparing a leech extracting solution; preparing a polymineral element solution; and mixing the leech extracting solution and the polymineral element solution according to the mass ratio of 5:(200-500) at 10-20 DEG C so as to obtain the multipurpose functional water. The preparation method utilizes ultrasonic-assisted extraction, so that the extraction efficiency of effective constituents of leech can be enhanced, and the waste of the raw material is reduced. According to the prepared multipurpose functional water, Fe, Cu, I and Mn can play a role of protecting the effective constituents in the leech extracting solution, so that the target concentration of medicinal components of the leech extracting solution can increase, and furthermore, the use effect of the solution is improved.

The invention provides a preparation method of high-FXa-resistant low-FIIa-resistant low-molecular heparin sodium. Common heparin is subjected to beta- elimination degradation in an organic solvent to reduce the falling of sulfonic groups from heparin, so that the preparation method has the advantages of mild degradation conditions and high anticoagulating activity of the product. The FXa-resistant titer of the low-molecular heparin sodium is 120-160IU / mg, the FIIa-resistant titer is 10-30IU / mg, and the average molecular weight is 3500-5000Da.

The present invention is concerned with a variant blood coagulation component, which is substantially homologous in amino acid sequence to a wild-type blood coagulation component capable of exhibiting anticoagulant activity in the protein C-anticoagulant system of blood and selected from protein C (PC) and activated protein C (APC), said variant component being capable of exhibiting an anticoagulant activity, that is enhanced in comparison with the anticoagulant activity expressed by the corresponding wild-type blood coagulation component, and said variant component differing from the respective wild-type component, in that it contains in comparison with said wild-type component at least one amino acid residue modification in its N-terminal amino acid residue sequence that constitutes the Gla-domain of protein C and at least one amino acid residue modification in the serine-protease domain of protein C. The present invention is also concerned with methods to produce such variants based on DNA technology; with DNA segments intended for use in the said methods; and with use of said variants for therapeutic and diagnostic purposes.

The invention provides a pharmaceutical composition containing active procyanidins and clopidogrel or the pharmacologically acceptable salt thereof. The procyanidins and the clopidogrel are used in a combined manner, a more effective method for curing thrombotic diseases is found, after trail for many times, the clopidogrel and the procyanidins (an extract of traditional Chinese medicine) are creatively and effectively combined together, the effect of applying the procyanidins to inhibit the thrombosis in a combined manner is discovered unexpected in the process of applying the clopidogrel to cure the thrombotic diseases, and not only the anticoagulant effect of the clopidogrel is free from the influence of the procyanidins, but also a good effect is achieved in the aspect of reducing the adverse reaction of bleeding after the use of the clopidogrel and the procyanidins in a combined manner, therefore, the risk of bleeding is greatly reduced when the advantages of good anticoagulant activity and fast effect of the clopidogrel during the antiplatelet aggregation are fully exerted, the risk of bleeding of the clopidogrel during the antiplatelet aggregation is effectively reduced and the adverse reaction of the clopidogrel is greatly reduced.

This invention relates to a medicine combination used to treat headache, which is made up of Chuanxiong and Tianma in a certain scientific weight proportion. The medicine combination can be made into any commonly used dosage form. The invention also provides other applications of this medicine combination in the production of an anti-oxidative drug, an antihypertensive drug, a platelet antiaggregation drug, an antithrombosis drug and an anticoagulant.

The invention discloses a preparation method of a sulfonated polypropylene non-woven fabric anticoagulant material, which belongs to the technical field of anticoagulant material preparation. The present invention uses konjac powder as a raw material, which is dispersed and extracted by ultrasonic waves, then precipitated with absolute ethanol, centrifuged, and the filtrate is ultrafiltered, subjected to secondary alcohol precipitation, combined and precipitated, vacuum freeze-dried, and then reacted with sulfuric acid esterification react with reagents, adjust the pH to obtain sulfated konjac glucomannan, and then soak the polypropylene non-woven fabric that has been irradiated and grafted in the sulfated konjac glucomannan solution to obtain sulfonated polypropylene non-woven fabrics. Spun anticoagulant material, the anticoagulant material of the present invention has good compatibility with blood, the life of anticoagulant is prolonged, the storage time of blood can be extended by 2-4 weeks, the incidence of thrombus is reduced by 40-60%, and there is Good application prospects.

The invention discloses a preparation method of a sea cucumber egg fermentation product with functions of lowering blood pressure and resisting blood coagulation. The preparation method of the sea cucumber egg fermentation product with the functions of lowering blood pressure and resisting blood coagulation comprises the following steps: washing sea cucumber eggs, and freeze-drying the washed sea cucumber eggs, so that freeze-dried sea cucumber egg powder is prepared for later use; mixing the freeze-dried sea cucumber egg powder with water, adding glucose, and adjusting the initial pH, so that a sea cucumber egg basic culture medium is prepared; sterilizing the basic culture medium; adding a bacillus natto bacterial suspension, and carrying out shaking culturing, so that a sea cucumber egg fermentation broth is prepared; centrifuging the sea cucumber egg fermentation broth, and collecting the obtained supernatant; and then, concentrating the supernatant, and freeze-drying the concentrate, so that the sea cucumber egg fermentation product freeze-dried powder with the functions of lowering blood pressure and resisting blood coagulation is prepared. The sea cucumber eggs are fermented with the bacillus natto according to the preparation method, so that fibrinolytic enzymes with the effect of dissolving thrombus are produced; moreover, polypeptides which are easy to absorb and have the functions of resisting blood coagulation and lowering blood pressure are also produced. The sea cucumber egg fermentation product can be directly eaten as a food, or prepared into health-care functional products after further developments, including concentrating, separating and so on.

The invention belongs to the technical field of medicine, and mainly relates to dabigatran cyclic derivatives as shown in a universal formula I or pharmaceutically acceptable salt thereof, a medicinal composition using the compounds as active ingredients, and application of the compounds and the medicinal composition to preparation of a thrombin inhibitorby.

The invention discloses fucosylation chondroitin sulfate oligosaccharide provided with a structure shown in J. The definition of a substituent group is shown in the description in detail. Besides, theinvention further discloses a preparation method of the fucosylation chondroitin sulfate oligosaccharide. According to the preparation method, chondroitin sulfate A salt is used as a raw material andis subjected to enzymolysis, protecting group operation and glycosylation sequentially, the oligosaccharide compound is synthesized, and the certainty of the structure enables the compound to be applied to the medicine field.

The invention discloses a preparation method of leech whole powder and relates to the technical field of medicine. The preparation method of the leech whole powder comprises the following steps: cleaning and disinfecting fresh poecilobdella manillensis, pulping, performing enzymolysis, removing peculiar smell, drying and the like. The extraction rate of hirudin of the obtained leech whole powder reaches up to 100 percent; meanwhile, the leech whole powder contains other active components of various tissues and saliva of the leeches and is conducive to improve the anti-coagulant activity of thehirudin; the leech whole powder can have flavors of milk flavor, fruit flavor or flower fragrance, has good taste and does not have peculiar smell.

The invention discloses a green algae polysaccharide and a preparation method thereof. The green algae polysaccharide takes alpha-L-(1,3)-connection rhamnose and alpha-L-(1,2)-connection rhamnose as a main chain, and beta-D-glucuronic acid is positioned to a marine sulfated polysaccharide with a novel structure of a branch chain. The green algae polysaccharide has obvious anticoagulation, fibrinolysis, thrombolysis and blood sugar and blood lipid reducing activities and can be used for preparing anticoagulation and blood sugar reducing medicines and health food. The green algae polysaccharide is derived from natural seaweeds, does not have toxic and side effects on human bodies, can prepare tablets, powder, granules, capsules and the like serving as anticoagulation medicines for preventing and treating thromboembolic diseases and blood sugar reducing medicines for preventing and treating diabetes mellitus by adding various attached agents, and has good market application prospect.

The invention discloses application of cucurbit[7]uril in a blood anticoagulation medicine.

The invention relates to sulphated compound microalgae polysaccharide. The sulphated compound microalgae polysaccharide is prepared by mixing sulphated chlorella polysaccharide and sulphated spirulinaon the base of the ratio of 1 to 16-16 to 1. The sulphated compound microalgae polysaccharide is capable of effectively inhibiting in-vitro proliferation of tumor cells, and is high in water solubility, so that the sulphated compound microalgae polysaccharide can play a role in vitro and is capable of inhibiting the growth of tumor.

The invention provides fluorine-containing benzimidazole compounds and a preparation method and application thereof. The fluorine-containing benzimidazole compounds have a structure represented in a formula I; the fluorine-containing benzimidazole compounds have a simple structure, good anticoagulant activity, and can prolong the time of blood coagulation, the preparation method of the fluorine-containing benzimidazole compounds is simple and efficient, and industrial production can be achieved easily.

A thrombus target fusion mA5UKB with both anticoagulating and thrombolytic functions is prepared from the mutant mAnxA5 of AnnexinA5 having anti-coagulating function and the urokinase B chain (UKB) having thrombolytic function through fusing. It can be used to prepare the thrombolytic medicine.