Preparation method of leech whole powder

The technology of leech and leech, which is applied in the field of medicine, can solve the problems of leech odor, poor taste, influence on patient compliance, etc., and achieve the effects of good taste and improvement of anticoagulant activity.

Inactive Publication Date: 2018-03-16
广西鹿帅仁生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Invention patent application 201510314664.X discloses a preparation method of leech powder rich in highly active hirudin. The prepared leech powder is generally taken with water, but the leech has a strong peculiar smell and a very bad taste, which directly affects the healt

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1:

[0024] The preparation method of this whole leech powder includes the following steps:

[0025] A. Cleaning and disinfection: Rinse 100kg of fresh Philippine bull leeches with clean water to remove debris, immerse in 50ppm chlorine dioxide aqueous solution for disinfection for 3 to 5 minutes, and drain for use;

[0026] B. Beating: Add 200kg of sterile water to the Philippine leeches obtained in step A to beat together, and set aside;

[0027] C. Enzymatic hydrolysis: pour the slurry obtained in step B into an enzymatic hydrolysis container, add 1 kg of chitinase and 0.3 kg of collagenase, and hydrolyze it for 1 hour at a pH of 4.0 and a temperature of 35-40°C to reach phenanthrene The body of the bovine leech is dissolved, and the enzyme is inactivated at 90℃ for 10 minutes;

[0028] D. Remove peculiar smell: add 200kg milk powder and stir evenly after cooling;

[0029] E. Drying: Adjust the pH value to 7 with 2mol / L NaOH solution, pre-cool for 240 min at -20, and t...

Example Embodiment

[0031] Example 2:

[0032] The preparation method of this whole leech powder includes the following steps:

[0033] A. Cleaning and disinfection: Rinse 100kg of fresh Philippine bull leeches with clean water to remove debris, immerse in 100ppm chlorine dioxide aqueous solution for disinfection for 3 to 5 minutes, and drain for use;

[0034] B. Beating: Add 200kg of sterile water to the Philippine leeches obtained in step A to beat together, and set aside;

[0035] C. Enzymatic hydrolysis: Pour the slurry obtained in step B into an enzymatic hydrolysis container, add 1 kg of chitinase and 0.6 kg of collagenase, and hydrolyze it for 2 hours at a pH of 5.0 and a temperature of 35 to 40°C to reach phenanthrene The body of the bovine leech is dissolved, and the enzyme is inactivated at 90℃ for 10 minutes;

[0036] D. Remove peculiar smell: After cooling, add 100kg milk powder and 1kg mogroside and stir evenly;

[0037] E. Drying: Adjust the pH value to 7 with 2mol / L NaOH solution, pre-cool f...

Example Embodiment

[0039] Example 3:

[0040] In the preparation method of this whole leech powder, in step D, add 100 kg of milk powder and 1 kg of steviol glycosides and stir evenly, and the other steps are the same as in Example 1.

[0041] The powder's anticoagulant activity was tested to be 573AT-U / g.

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PUM

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Abstract

The invention discloses a preparation method of leech whole powder and relates to the technical field of medicine. The preparation method of the leech whole powder comprises the following steps: cleaning and disinfecting fresh poecilobdella manillensis, pulping, performing enzymolysis, removing peculiar smell, drying and the like. The extraction rate of hirudin of the obtained leech whole powder reaches up to 100 percent; meanwhile, the leech whole powder contains other active components of various tissues and saliva of the leeches and is conducive to improve the anti-coagulant activity of thehirudin; the leech whole powder can have flavors of milk flavor, fruit flavor or flower fragrance, has good taste and does not have peculiar smell.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a preparation method of whole leech powder. Background technique [0002] Phnom Penh leech, Poecilobdellamanillensis, a leech family animal, is a medicinal material used by Zhuang doctors in Guangxi. Its common names are leeches, leeches, horse turtles, and water hemp sticks. (Amenorrhoea), Bainong (sore carbuncle), Mabang (stroke), twist image (sprain and contusion) and other diseases. According to literature records, this product mainly contains natural hirudin and histamine-like compounds, among which hirudin has the functions of anticoagulation, fibrinolysis, inhibition of platelet aggregation, reduction of whole blood viscosity, reduction of blood fat, and reduction of thrombus. [0003] Natural hirudin is a single-chain small-molecule acidic single-chain polypeptide with 65 or 66 amino acid residues and no glycosylation extracted from the secretion of leech salivary gland...

Claims

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Application Information

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IPC IPC(8): A61K35/62A61K9/19A61K47/46A61K47/44A61P15/00A61P17/02A61P9/10A61P29/00
CPCA61K35/62A61K9/0053A61K9/19A61K47/44A61K47/46
Inventor 覃春花覃海南
Owner 广西鹿帅仁生物科技有限公司
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