35 results about "Poecilobdella manillensis" patented technology

The invention discloses an extract method of the effective constituent of the Natural hirudin. The method is that the blood-sucking leech is washed cleanly by water and is added with the ethanol water with content of 15 per cent to 25 per cent to soak for 30h to 50h, and then is filtered to gain the filter liquor and dregs of a decoction; the Poecilobdella manillensis waste residue is broken into pieces and is added with aqueous acetone solution with content of 15 per cent to 25 per cent to soak, and then is filtered to gain the filter liquor; after recovering ethanol and acetone from the two-time filter liquor in the vacuum, the filter liquor is combined and frozen and dried in the vacuum conventionally to gain the product. The invention is simple in process, low in cost, higher in yield, good in product quality and long in retention period. The ''three wastes'' are not discharged in the whole production process, and the product can work as the raw material in such three fields as medicine, hairdressing and health food.

The invention discloses a poecilobdella manillensis indoor breeding device. The poecilobdella manillensis indoor breeding device mainly comprises a breeding box, a mesh bag and a multi-layer breeding rack, wherein the mesh bag and the multi-layer breeding rack are arranged in the breeding box in a matched mode. On the basis, an artificial blood sucking poecilobdella manillensis indoor breeding method is established based on the biological nature of the poecilobdella manillensis, the mature poecilobdella manillensis breeding is carried out through the indoor multi-layer breeding rack/box, the escaping preventing mesh bag, a manual feeding platform and a water storage temperature control system, and the poecilobdella manillensis larva can be directly bred into the mature individual. By means of the device and method, the occupied space is small, the unit area yield/cultivation benefit which is 2-4 times that of existing indoor pool cultivation is large, the breeding survival rate is more than 85 percent and is improved by 15 percent compared with an ordinary method, the breeding period is shortened by one to two months, and the safety quality of the matured poecilobdella manillensis is high.

The invention relates to a hirudin production process that a Hirudinaria manillensis refrigerated fresh body is taken as raw material, which belongs to the pharmaceutical field of the traditional Chinese medicine, and relates to the active constituent extraction of animals. The invention aims at solving the problem that the traditional hirudin extraction process is difficult to be applied to the industrialized production. The process adopts the method that the hirudin is extracted from the whole Hirudinaria manillensis, thus the industrialized production can be operated. The Hirudinaria manillensis is rectified, and the pulp is distilled for three times with normal saline, and filtered. The filtrate is boiled and filtered, and protein is removed from the filtrate. The filtrate is collected, and after freezing, concentration and alcoholization, and the hirudin crude product is obtained. The hirudin crude product is washed with the normal saline, and then washing solution is concentrated, and finally the product is obtained through freezing and drying. The production process method of the hirudin is simple, and the quality of the end product is qualified. The pharmacology experiment result indicates that the hirudin extracted by utilizing the process has good pharmacological action.

The invention discloses a preparation method of whole leech meal rich in high-activity hirudins. The preparation method comprises the following steps: by taking fresh poecilobdella manillensis as a raw material, washing by clear water to remove impurities, soaking in 50-100ppm of chlorine dioxide aqueous solution and disinfecting for 3-5min, adding sterile water which is 2-3 times of the weight of poecilobdella manillensis and homogenizing, then hydrolyzing using chitinase and collagenase for 1-2h under the conditions of a pH value of 4.0-5.0 and a temperature of 35-40 DEG C until poecilobdella manillensis bodies are dissolved, heating to 90 DEG C and carrying out enzyme deactivation for 10min, cooling and then adjusting the pH value to 7, then carrying out vacuum freeze drying to obtain the whole leech meal. By using the double-enzyme method, the whole bodies comprising salivary glands are hydrolyzed, so that all hirudins can become free states, all the hirudins in leech bodies are released, and the absorption and utilization of human body on the hirudins are prompted. Meanwhile, the whole leech meal contains other active ingredients in the various tissues and saliva of leeches, and improvement for the anti-coagulant activity of the hirudins is promoted.

The invention discloses a specific primer for identifying poecilobdella manillensis based on a molecular biological method. An upstream primer is 5'-TCTGGCTCTTAGGCTTCG-3', and a downstream primer is 5'-GTTGTCTTCCTGGCTGTT-3'. A method for quickly identifying poecilobdella manillensis based on amplification of the specific primer comprises the following main steps: (1) extracting total DNA of a sample to be detected to obtain extracted genome DNA; (2) performing PCR (Polymerase Chain Reaction) amplification by taking the genome DNA extracted in the step (1) as a template and adopting the upstream primer and the downstream primer according to claim 1; and (3) performing agarose gel electrophoresis detection and gel imaging on the reaction product obtained in the step (2) to realize specific identification of poecilobdella manillensis, thereby providing a reference for a molecular biological identification method of poecilobdella manillensis.

The invention relates to the field of biotechnologies, and especially relates to a leech-specific nutritional induction solution, and a method for extracting natural hirudin. The leech-specific nutritional induction solution includes sodium chloride, compound amino acids, glucose, trans-4,5-epoxy-(E)-2-decenal and sterile water. A technology for satiating Poecilobdella manillensis Lesson by usingliving body induction is adopted to avoid large-scale killing of leeches and make the leeches reused. Feeding of the specific nutritional induction solution instead of whole bovine blood and other induction substances with poor palatability can induce the full intake of leeches and reduce the mixing of a large amount of blood and other impurities, so the subsequent separation and purification of natural hirudin are benefited.

The invention provides a determination method of fingerprint of Poecilobdella manillensis. Poecilobdella manillensis, commonly known as yellow-stripped leech, belongs to Arhynchobdellida, Hirudinidae and Poecilobdella, has evident activities on anticoagulation, antithrombosis, thrombolysis and platelet aggregation resistance, and has evident medicinal value. Up to now, no pharmacopeia or local standard records Poecilobdella manillensis, and no reports on fingerprint of Poecilobdella manillensis are present. Poecilobdella manillensis material is determined by efficient capillary electrophoresis, a fingerprint of the Poecilobdella manillensis material is established, and a method of overall quality estimation on the Poecilobdella manillensis material is provided and is used to control the quality of the Poecilobdella manillensis material.

The invention discloses a water nutrient for Poecilobdella manillensis and relates to the technical field of Poecilobdella manillensis culture. The water nutrient for Poecilobdella manillensis comprises, by weight, 1 to 3 parts of sodium humate, 1 to 2 parts of a bacillus composite preparation, 1 to 2 parts of a photosynthetic bacterial preparation, 1 to 2 parts of a lactobacillus preparation and1 to 2 parts of composite trace elements. The water nutrient improves Poecilobdella manillensis culture water quality. After applying the water nutrient for Poecilobdella manillensis, growth and breeding of harmful microorganisms, harmful algae and aquatic plants in the culture water are inhibited, water transparency and dissolved oxygen content of the water are increased, water is purified, organic matters in water are degraded and transformed into available inorganic nutrient salts and trace elements are continuously added into water so that nutrients such as abundant trace elements and vitamins are provided for cultured Poecilobdella manillensis, promote the healthy growth of Poecilobdella manillensis and improve Poecilobdella manillensis quality.

The invention discloses a processing method of poecilobdella manillensis and belongs to the technical field of traditional Chinese medicine. The processing method of the poecilobdella manillensis comprises steps as follows: step 1, freezing; step 2, soaking; step 3, placement; step 4, bloodletting; step 5, freeze drying and powdering. According to the processing method of the poecilobdella manillensis, the problem of activity reduction of high-temperature antithrombin is solved, the fishiness removal and effect reservation are realized, active ingredients in the poecilobdella manillensis can be maintained to the greatest extent, and the curative effect of a product is also guaranteed to the greatest extent while acceptability of the product by a patient is improved.

The invention discloses a medical hirudin content improving poecilobdella manillensis industrialized intensive breeding method and a temporary breeding box. The medical hirudin content improving poecilobdella manillensis industrialized intensive breeding method comprises the following steps of cleanly breeding poecilobdella manillensis weighing more than 4 grams, dividing the cleanly bred poecilobdella manillensis by gram weight into five groups, proportionally intensively culturing the poecilobdella manillensis in a container containing 10 kg of purified water for 3-6 months, cleaning and nursing the poecilobdella manillensis every day to increase the content of hirudin in the poecilobdella manillensis to 300-900 ATU/G, and loading the poecilobdella manillensis into the special temporarybreeding box for standby application or transportation. The temporary breeding box is composed of a box body and a box cover, the inside of the box body is separated into 4-8 cells by a grating, the box cover is provided with exhaust holes, and the inside of the box body is provided with a moisturizing layer. The medical hirudin content improving poecilobdella manillensis industrialized intensivebreeding method is novel in process, low in cost, high in industrialization degree, free from limitations such as weather and place and applicable to scale production. The living poecilobdella manillensis can be transported by the special temporary breeding box as the carrier and directly applied to living body therapy in Chinese medical clinics; freeze-dried poecilobdella manillensis products canbetter meet the requirements of modern biological characteristic pharmaceutical factories on quality.

The invention discloses a method for breeding Poecilobdella manillensis. The method includes the steps of: constructing a breeding pond, maintaining a stocking amount of 60,000-70,000 Poecilobdella manillensis per mu of water surfaces, placing 20-30 kilograms of snails per mu of water surfaces once so as to enable Poecilobdella manillensis to reproduce naturally, and on the basis of the natural feed, placing animal blood clots once a week for Poecilobdella manillensis feeding; keeping water change, maintaining the water temperature at 10-35 DEG C and a certain amount of dissolved oxygen; pouring a bleach solution with the mass concentration of ten thousandth into a whole pool; and configuring escape-proof harm-preventing facilities, and performing overwintering management and disease prevention well. According to the method, artificial feeding of the fresh animal blood clots is conducted based on the natural feed, the survival rate of Poecilobdella manillensis is high, the growth rateof Poecilobdella manillensis can be improved obviously and quickly, a lowest feed conversion rate is obtained, and the economic benefit is good.

The invention relates to a dried animal casing suitable for being fed to hematophagous medical leeches. The dried animal casing is processed from one of chitterlings, beef intestines or sheep intestines and can be fed to hematophagous leeches such as leeches, Poecilobdella manillensis and the like. The dried animal casing is processed by the steps of taking out casing layers from intestines of animals, soaking thoroughly washed small intestines of the animals into clean water, peeling grease, serous coats and fascia from outsides of intestinal canals, thoroughly flushing, putting the degreased casings into a clean water jar, repeatedly and continuously cleaning by hands so as to thoroughly remove swage and grease, forming by virtue of a mold, and finally carrying out blowing drying. By taking the dried animal casing as a feed material of the hematophagous leeches, the utilization rate of the feed is increased, the ingestion is improved, the growth is promoted, and residual feeds are reduced.

The invention discloses a device and method for feeding poecilobdella manillensis manually. The device comprises a container and a pipe, wherein an opening is formed in the container, a heating and heat insulating device is arranged in the container, one end of the pipe is communicated with the bottom of the side face of the container through a control valve, and the other end of the pipe is detachably connected with a closed casing. The method for feeding the poecilobdella manillensis with the device comprises the steps of (1) placing blood in the container, and starting the heating and heat insulating device to enable the temperature of the blood to be kept between 36 DEG C and 39 DEG C; (2) opening the control valve and a selection valve to enable the casing to be filled with the blood, and feeding the hungry poecilobdella manillensis with the casing filled with the blood after it is ensured that no leakage exists through detection; (3) closing the control valve and the selection valve after the poecilobdella manillensis eats for 10-30 minutes, taking the used casing, cleaning the poecilobdella manillensis, and placing the poecilobdella manillensis back into a culture pond to be bred continuously. The device and method solve the problems of low feeding efficiency and waste of food and increase culture benefits.

The invention provides a composition for medicinal material deodorization. The composition comprises a preparation prepared from, by weight, 5-15 parts of chrysanthemum flowers and 7-21 parts of cyclodextrin. The invention further provides a preparation method and application of the composition. By means of the prepared composition, the fishy smells of poecilobdella manillensis freeze-dried powder can be obviously reduced, the poecilobdella manillensis freeze-dried powder can keep high anticoagulant activity, and the beneficial effects of being efficient, safe, free of toxins, free of pollution, simple, easy to implement and the like are achieved.

The invention provides an improved poecilobdella manillensis medicinal material activity detection method, which comprises the following steps: taking 0.5 g of poecilobdella manillensis medicinal material, precisely weighing, precisely adding 15 ml of 0.9% sodium chloride solution, fully stirring, leaching for 30 minutes, shaking and filtering in real time, precisely measuring 100 microliters of subsequent filtrate, three times, respectively putting into three test tubes, respectively marking the test tubes as A, B and C, and respectively marking the test tubes as B, C and D; the method comprises the following steps: respectively adding 200 [mu] l of a tris (hydroxymethyl) aminomethane hydrochloric acid buffer solution containing 0.5% of fibrinogen, uniformly shaking, placing 3 parts of tubes to be tested in a water bath, carrying out warm soaking at 37 +/-0.5 DEG C for 5 min, and dropwise adding a thrombin solution containing 40 units per 1 ml. According to the method, the anticoagulant activity of the poecilobdella manillensis medicinal material is rapidly and accurately detected by increasing the dosage multiples of the parallel sample and the thrombin solution within the capacity range of a laboratory technician, and a set of relatively complete operation judgment process is designed.

A fishy smell removal treatment method for leeches comprises the following steps: work before processing treatment, pretreatment, bloodletting treatment, freeze-drying treatment, crushing, body fishysmell removal and secondary freeze-drying molding. According to the method, the fishy smell of a poecilobdella manillensis product can be effectively removed, meanwhile, the taste of the product can be improved, the influence on the hirudin (anticoagulant component) component contained in the poecilobdella manillensis and the activity of the hirudin is small, and further development of the poecilobdella manillensis product is facilitated.

The invention provides a greenhouse overwintering method for artificially-bred poecilobdella manillensis lesson. The method includes steps: 1) digging a pit in a greenhouse, arranging an overwinteringcontainer matched with the pit in the pit, and laying soil at the bottom of the overwintering container to form a slope at the bottom of the overwintering container; 2) adding water to the bottom ofthe overwintering container to keep the soil wet, and releasing poecilobdella manillensis lesson to the soil surface to allow the poecilobdella manillensis lesson to freely enter the soil; 3) in an overwintering period, controlling the temperature of the greenhouse and the temperature of the soil, and taking out poecilobdella manillensis lesson after overwintering is completed. The method has advantages of easiness in operation, sharp reduction of labor, high overwintering survival rate and the like.

The invention discloses a poecilobdella manillensis anticoagulant crude product extraction method, which comprises: selecting a poecilobdella manillensis living body in a starved state, placing in an environment of 38-40 DEG C, and feeding until the poecilobdella manillensis living body automatically falls off; quickly transferring the fallen poecilobdella manillensis living body to a plate surface at the temperature of-18 DEG C to-5 DEG C, and stimulating the poecilobdella manillensis to spit out the solution in the body to obtain an anticoagulant solution; and placing the anticoagulant solution in an environment of 0-15 DEG C, and sequentially performing filtration, protein separation and precipitation, concentration treatment and freeze drying to obtain the poecilobdella manillensis anticoagulant crude product. According to the method, the poecilobdella manillensis living body raw material can be efficiently and repeatedly utilized, and the obtained poecilobdella manillensis anticoagulant crude product is convenient for impurity removal and purification.

The invention discloses a formula for cardiovascular and cerebrovascular diseases and a preparation method and application thereof.The formula is prepared from 0.6%-12% of poecilobdella manillensis freeze-dried powder, 2%-4% of lipoic acid, 30%-42% of L-arginine, 30%-42% of L-citrulline, 2%-6% of pseudo-ginseng extract and 15%-35% of auxiliary materials, wherein the sum of the content percentages of all the components is equal to 100%. The poecilobdella manillensis freeze-dried powder and the pseudo-ginseng product are combined for administration, so that the poecilobdella manillensis freeze-dried powder has an extremely good treatment effect on cardiovascular and cerebrovascular diseases, can remarkably improve or eliminate uncomfortable symptoms caused by the cardiovascular and cerebrovascular diseases and refractory hypertension, can take effect in about 10 days without an operation, and is stable in effect.

The invention discloses a culture method for Poecilobdella manillensis. The method comprises the following steps: (1) preparing culture cages: placing net cages into glass jars, and carrying out disinfecting, thereby preparing the culture cages; (2) stocking Poecilobdella manillensis offspring into the culture cages, adding water into the culture cages, changing the water once every 2 to 3 days, feeding feed to the culture cages, and carrying out culturing at the ambient temperature of 25 DEG C to 26 DEG C and the relative humidity of 50% to 60%; and (3) carrying out harvesting after carrying out culturing for 4 to 5 months. The culture method disclosed by the invention is high in yield and good in economic benefit.

The invention discloses a method for preparing an active extract hair-growing product by taking freeze-dried poecilobdella manillensis freeze-driedpowder as a raw material. The method comprises the following steps: S1, weighing and homogenizing raw materials to obtain slurry; S2, freezing and drying the slurry to obtain a dried material; S3, crushing the dried material, and screening the crushed material to obtain freeze-dried powder; S4, adding water into the freeze-dried powder, conducting filtering, and collecting filtrate; S5, collecting an active extracting solution through the filtrate;and S6, performing final treatment on the active extracting solution to obtain a final finished product. The method provided by the invention is used for extracting the freeze-dried poecilobdella manillensis freeze-dried powder; the extract has very high activity and can be used for preparing the high-activity hair-growing product; good action efficiency is ensured, generally, the effect can be basically achieved after use for 30-60 days; the product is smeared on a part with less hair amount of a user once a day, the product is continuously used for 30 days, the hair amount is increased to 120-140 pieces/cm<2> from 70-90 pieces/cm<2>, the hair amount is increased to 150-180 pieces/cm<2> after the product is continuously used for 60 days, and the hair hardness is increased and obviously improved.