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Method for extracting poecilobdella manillensis anticoagulant crude product

An extraction method and leech technology are applied in the directions of blood diseases, pharmaceutical formulations, medical preparations containing active ingredients, etc., which can solve the problems of increasing the difficulty of removing impurities and purification, multiple raw materials, inconvenient production and use, etc., and achieve easy feeding. The effect of management, cost reduction and difficulty reduction

Pending Publication Date: 2022-07-05
广西蛭郎中生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, due to differences in living environments, feeding conditions and ages of Hirudo phenanthae, the content of natural antithrombin, the main medicinal ingredient contained in Hirudo phenanthae, varies greatly, resulting in the inability to standardize the raw materials , which in turn caused a lot of inconvenience in actual production and use
In addition, the existing method for obtaining natural antithrombin is mainly to kill Hirudo phenanthrene directly as a raw material of medicine, which consumes more raw materials, and the cost remains high; the crude antithrombin in the obtained raw material There will be more impurities, which increases the difficulty of impurity removal and purification

Method used

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  • Method for extracting poecilobdella manillensis anticoagulant crude product
  • Method for extracting poecilobdella manillensis anticoagulant crude product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for extracting an anticoagulant crude product of leech, comprising the following steps:

[0035] Step 1. Select 20 kilograms of 2-year-old healthy, purified and starved leech live bodies, place them in an environment of 38 to 40°C, and use chicken blood at 35 to 37°C to feed them to the live bodies of Philippine burdocks on their own. fall off.

[0036] Step 2: Quickly transfer the fallen leech live body to a plate surface at minus 5°C, and stimulate the leech to spit out the body solution to obtain an anticoagulant solution.

[0037] Step 3: Select the still active leech from the spit, place it in an environment of 38-40°C, and use chicken blood at 35-37°C to continue feeding until the living body of the leech falls off on its own, and then Quickly transfer the exfoliated living leech to the plate at minus 5°C, and stimulate the leech to spit out the body solution to obtain an anticoagulant solution. Repeat the above operation until all leech are dead.

[0...

Embodiment 2

[0041] A method for extracting an anticoagulant crude product of Hirudo philippines, comprising the following steps:

[0042] Step 1. Take 200 liters of physiological saline with a concentration of 0.9%, add 2 kg of glucose, 1 kg of compound amino acids, 1 kg of vitamin C, and 6 kg of chicken serum albumin powder, and stir evenly to obtain an artificial solution. The temperature of the prepared solution is 35-37°C, and the artificially prepared solution is placed in an environment of 38-40°C for use.

[0043]Step 2: Select 20 kilograms of 2-year-old healthy, purified and starved leech live bodies, and place them in an artificially prepared solution for feeding until the live bodies of Philippine burdocks fall off on their own.

[0044] Step 3: Quickly transfer the fallen leech live body to a plate surface at minus 5°C, and stimulate the leech to spit out the solution in the body to obtain an anticoagulant solution.

[0045] Step 4. Select the still active leech from the spit,...

Embodiment 3

[0048] On the basis of Example 2, chicken serum albumin powder was replaced with bovine serum albumin powder.

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Abstract

The invention discloses a poecilobdella manillensis anticoagulant crude product extraction method, which comprises: selecting a poecilobdella manillensis living body in a starved state, placing in an environment of 38-40 DEG C, and feeding until the poecilobdella manillensis living body automatically falls off; quickly transferring the fallen poecilobdella manillensis living body to a plate surface at the temperature of-18 DEG C to-5 DEG C, and stimulating the poecilobdella manillensis to spit out the solution in the body to obtain an anticoagulant solution; and placing the anticoagulant solution in an environment of 0-15 DEG C, and sequentially performing filtration, protein separation and precipitation, concentration treatment and freeze drying to obtain the poecilobdella manillensis anticoagulant crude product. According to the method, the poecilobdella manillensis living body raw material can be efficiently and repeatedly utilized, and the obtained poecilobdella manillensis anticoagulant crude product is convenient for impurity removal and purification.

Description

technical field [0001] The invention belongs to the technical field of leeches processing, in particular to a method for extracting crude anticoagulant products of leech. Background technique [0002] The leech is a kind of blood-sucking medical leech animal, which is rich in natural antithrombin and is used to prepare medicines for the treatment and prevention of cardiovascular and cerebrovascular diseases, and the leech has been successfully included in the "Guangxi Zhuang Autonomous Region Zhuang Medicinal Materials". Quality Standard", which provides a basis for the legal utilization of leech as a medicinal material. [0003] However, due to the difference in living environment, feeding conditions and the age of leech, the content of natural antithrombin, the main medicinal ingredient contained in leech, varies greatly, resulting in the inability to standardize the raw materials. , which leads to a lot of inconvenience in actual production and use. In addition, the exi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/62A61K38/58A61P7/02A01K61/40
CPCA61K35/62A61K38/58A61P7/02A01K61/40Y02A40/81
Inventor 覃壬李洪贺嘉允
Owner 广西蛭郎中生物科技有限公司
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