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Determination method of fingerprint of Poecilobdella manillensis

A technology of fingerprint and Hirudo phenatus, which is applied in the field of determination of the fingerprint of Hirudo phenatus medicinal material

Active Publication Date: 2015-04-15
HEBEI YILING MEDICINE INST
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Problems solved by technology

[0002]The Philippine leech (Poecilobdella manillensis), commonly known as the Phnom Penh leech, belongs to the order Anoscillidae, the family Hirudidae, and the genus Poecilobdella manillensis. It is mainly distributed in Guangxi, Guangdong, Hainan, Fujian and other provinces, and can be artificially cultured, it has significant anticoagulant, antithrombotic, thrombolytic, antiplatelet aggregation activities, the main chemical components are fatty acids, amino acids and proteins, and the reported active components are leech Hirudin P6, hirudin P18, hirudin A and hirudin B, the acute toxicity test shows that hirudin has no obvious side effects, so it has significant medicinal value, so far no pharmacopoeia and local standards There is no report about the fingerprint of Hirudo phenanthae

Method used

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  • Determination method of fingerprint of Poecilobdella manillensis
  • Determination method of fingerprint of Poecilobdella manillensis
  • Determination method of fingerprint of Poecilobdella manillensis

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Embodiment 1

[0053] The preparation method of the test solution: the dry product of Hirudo phenanthrene was crushed through the No. 4 pharmacopoeia sieve, 1.0g of the sample was weighed, accurately weighed, and 6ml of deionized water was added accurately, mixed and ultrasonicated for 30min, centrifuged, and the supernatant was passed through 0.22 μm filter membrane, the filtrate is used as the test solution for high performance capillary electrophoresis;

[0054] Electrophoresis conditions: a quartz capillary column with an inner diameter of 75 μm and an effective column length of 50 cm is used as a separation channel, and the buffer is 25 mmol / LNa 2 HPO 4 -120mmol / L Tris-16mmol / L sodium dodecyl sulfate, adjust the pH value to 12.0 with 1 mol / L NaOH, operating voltage 17KV, column temperature 25°C, detection wavelength 254nm, injection parameters 0.5psi ×6sec, running time 27min.

[0055] Get the test solution, according to the above electrophoresis conditions, sample injection, record t...

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Abstract

The invention provides a determination method of fingerprint of Poecilobdella manillensis. Poecilobdella manillensis, commonly known as yellow-stripped leech, belongs to Arhynchobdellida, Hirudinidae and Poecilobdella, has evident activities on anticoagulation, antithrombosis, thrombolysis and platelet aggregation resistance, and has evident medicinal value. Up to now, no pharmacopeia or local standard records Poecilobdella manillensis, and no reports on fingerprint of Poecilobdella manillensis are present. Poecilobdella manillensis material is determined by efficient capillary electrophoresis, a fingerprint of the Poecilobdella manillensis material is established, and a method of overall quality estimation on the Poecilobdella manillensis material is provided and is used to control the quality of the Poecilobdella manillensis material.

Description

technical field [0001] The invention relates to a method for determining the fingerprint of Hirudo phenanthae medicinal material, which belongs to the technical field of drug analysis. Background technique [0002] The Philippine leech (Poecilobdella manillensis), commonly known as the Phnom Penh leech, belongs to the genus Hirudidae, Hirudidae, and the genus Hirudo. Significant anticoagulant, antithrombotic, thrombolytic, and antiplatelet aggregation activities, the main chemical components are fatty acids, amino acids and proteins, of which the active components have been reported as hirudin P6, hirudin P18, hirudin A and phenanthrene Hirudin B, the acute toxicity test shows that Hirudinus phenatus has no obvious toxic and side effects, so it has significant medicinal value. So far, it has not been recorded in the Pharmacopoeia and local standards, and there is no report about the fingerprint of Hirudinium phenatus. Contents of the invention [0003] The purpose of the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N27/447
Inventor 姜新刚王宗权贾继明赵韶华何慧民马静杨超
Owner HEBEI YILING MEDICINE INST
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