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Method for measuring finger-print of poecilobdella manillensis drug

A technology of fingerprints and determination methods, which is applied in the field of determination of fingerprints of the medicinal material Hirudo phenatus

Active Publication Date: 2013-02-06
HEBEI YILING MEDICINE INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002]The Philippine leech (Poecilobdella manillensis), commonly known as the Phnom Penh leech, belongs to the order Anoscillidae, the family Hirudidae, and the genus Poecilobdella manillensis. It is mainly distributed in Guangxi, Guangdong, Hainan, Fujian and other provinces, and can be artificially cultured, it has significant anticoagulant, antithrombotic, thrombolytic, antiplatelet aggregation activities, the main chemical components are fatty acids, amino acids and proteins, of which the active ingredient reported is leech Hirudin P6, hirudin P18, hirudin A and hirudin B, acute toxicity test shows that hirudin has no obvious side effects, so it has significant medicinal value, so far no pharmacopoeia and local standards and there is no report on fingerprints

Method used

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  • Method for measuring finger-print of poecilobdella manillensis drug

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Experimental program
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Embodiment 1

[0049] The preparation method of the test solution: crush the dry product of Hirudo phenanthae, pass through a 40-mesh sieve, take 0.40g, add 5ml of deionized water for ultrasonication for 15min, centrifuge at 4500r min-1 for 15min, filter with a 0.45μm filter membrane, and the filtrate is used as a high-efficiency Liquid chromatography need testing solution standby;

[0050] Chromatographic conditions: The chromatographic column is a C18 column, the specification is 250mm×4.6mm, 5μm; the mobile phase: the A phase is acetonitrile, the B phase is water, gradient elution; the elution program is 0→5min, A is 0%, 5→ 23min, A is 0%→4%, 23→24min, A is 4%→5%, 24-66min, A is 5%→21.4%; flow rate: 1ml min -1 ; Column temperature: 20°C; detection wavelength is 254nm.

[0051] Take the test solution, inject samples according to the above-mentioned chromatographic conditions, record the chromatogram, and compare it with the standard chromatogram, and it is completely fitted.

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Abstract

The invention provides a method for measuring a finger-print of a poecilobdella manillensis drug. Poecilobdella manillensis (poecilobdella manillensis) is commonly referred to as a phnom penh leech, belongs to arhynchobdellida, hirudinidae and poecilobdella, has the obvious functions of resisting coagulation, resisting thrombus, thrombolysis, and antiplatelet aggregative activity, has significant medical value, has not recorded in pharmacopoeia or other local standards so far, and has no any report about the finger-print. According to the method disclosed by the invention, the poecilobdella manillensis drug is measured by a high-performance liquid chromatography; the finger-print of the poecilobdella manillensis drug is built; a method is provided for entire quality evaluation of the drug, and the method is used for quality control of the poecilobdella manillensis drug material.

Description

Technical field [0001] The present invention involves a measurement method of a fingerprint map of the Philippines, which belongs to the field of drug analysis technology. Background technique [0002] Poecilobdella Manillensis, commonly known as Phnom Penh locust, belongs to the genus without kissy, medical gimmicks, and cattle, mainly distributed in provinces such as Guangxi, Guangdong, Hainan, and Fujian.Anti -coagulation, anti -thrombosis, thrombosis, and anti -platelet gathering activity. The main chemical components are fatty acids, amino acids, and proteins.The acute toxic test shows that the Philippines has no obvious toxic and side effects, so it has significant medicinal value. So far, the pharmacopoeia and local standards are not collected, and there are no reports about the fingerprint map. Invention content [0003] The purpose of the present invention is to provide a method of determining the fingerprint map of the Filipino cattle. [0004] The measurement method o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 孟作环马振嗣乔莉李文烈贾继明宋剑
Owner HEBEI YILING MEDICINE INST
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