432 results about "Drug analysis" patented technology

The invention relates to the field of pharmaceutical analytical chemistry, in particular to a method for determining tandospirone and salt intermediates thereof, high performance liquid chromatography is adopted, and chromatographic conditions include that a chromatographic column with octadecylsilane chemically bonded silica as a filler is adopted, and gradient elution is performed by a mobile phase A and a mobile phase B; wherein the mobile phase A consists of a first water phase and acetonitrile; the mobile phase B is composed of a second water phase and acetonitrile; the first water phase is a water solution containing monopotassium phosphate and sodium hexanesulfonate, and the pH value of the first water phase is 3.0-3.8; the second water phase is an aqueous solution containing monopotassium phosphate and sodium hexanesulfonate, and the pH value of the second water phase is 1.8-2.6. The testing method disclosed by the invention has good specificity, accuracy and repeatability, and can be used for monitoring the quality of the tandospirone raw material medicine.

The invention relates to the technical field of medicine analysis and traditional Chinese medicine detection, in particular to a construction method, a detection method and application of a specific chromatogram of a szechuan lovage rhizome perfume extract. Wherein the conditions of the liquid chromatographic analysis are as follows: gradient elution is carried out by adopting an aqueous solution of a mobile phase A acetonitrile and a mobile phase B phosphoric acid for 0-5 minutes, the volume percent of the mobile phase A is maintained to be 4% and 5-12 minutes, the volume percent of the mobile phase A is increased to 16% from 4% and 12-19 minutes, the volume percent of the mobile phase A is maintained to be 16% and 19-29 minutes, the volume percent of the mobile phase A is increased to 40% from 16% and 29-31 minutes, and the volume percent of the mobile phase A is maintained to be 4% and 12-19 minutes. The volume percentage of the mobile phase A is increased to 55% from 40%, 31-37 min, and the volume percentage of the mobile phase A is maintained to be 55%. The method can be used for quantitatively determining the water-soluble components of the szechuan lovage rhizome perfume extract, and can also be used for identifying the true and false.

The present invention belongs to the field of pharmaceutical analysis technology, and specifically relates to a method for detecting related substances in 2-aminothiophenol. The present invention provides a method for detecting related substances in 2-aminothiophenol, and provides a reverse-phase HPLC assay method that can detect related substances in 2-aminothiophenol. The method has good specificity, linearity, precision, sensitivity, accuracy, and durability, and is suitable for quality control of 2-aminothiophenol.

The invention discloses a multi-technology combined medication analysis platform of an oligonucleotide drug, which relates to the technical field of drug analysis and is technically characterized by comprising an oligonucleotide drug delivery technology module, a liposome, a polymer nanoparticle or an exosome is selected as a delivery carrier, the surface of the delivery carrier is modified with hydrophilic polyethylene glycol or a targeting ligand, and the oligonucleotide drug delivery technology module is connected with the oligonucleotide drug delivery module. The performance of the material is represented by dynamic light scattering and a transmission electron microscope; the multi-technology platform analysis module integrates RT-qPCR, LC-FL, LC-MS / MS and LC-HRMS technologies, is respectively used for target gene expression quantification, drug distribution tracking, drug principal component quantification and metabolite structure analysis, establishes a cross validation rule, and requires a correlation coefficient R2gt of an LC-MS / MS quantitative result and RT-qPCR expression data; 0.95%, 0.95%; the pharmacokinetic evaluation module constructs a PBPK model to predict human pharmacokinetic parameters, and draws a drug metabolism network diagram in combination with an LC-HRMS metabolite identification result; by integrating various advanced analysis technologies, the problems of low sensitivity, poor specificity and difficult metabolite analysis in the prior art are solved.

The invention provides a method for detecting dichromate radicals based on surface enhanced Raman spectroscopy, and belongs to the technical field of analytical chemistry. Comprising the following steps: mixing a to-be-detected sample with a sulfydryl-containing compound solution, and adjusting to an acidic condition, so that dichromate Cr (VI) and the sulfydryl-containing compound are subjected to an oxidation-reduction reaction; constructing an SERS substrate, namely preparing a ZnO / Ag heterostructure substrate; mixing the suspension of the ZnO / Ag heterostructure substrate with a reaction system to obtain a mixed solution, so that the ZnO / Ag heterostructure substrate adsorbs a sulfydryl-containing compound and enhances a Raman signal; dropwise adding the mixed solution to the surface of a solid carrier, acquiring a Raman spectrum signal by using a Raman spectrometer, and quantifying the Cr (VI) concentration through the change of signal intensity. The method realizes rapid and high-sensitivity detection of Cr (VI), has strong selectivity and anti-interference capability, and is suitable for detection in the fields of environmental monitoring, food safety, pharmaceutical analysis and the like.

The invention relates to the technical field of pharmaceutical analysis, in particular to a thin-layer chromatography identification method of vitality-preserving decoction and a preparation thereof. Comprising the following steps: S1, extracting a sample to be detected by using diethyl ether, and preparing a test solution; s2, preparing a cinnamaldehyde reference substance solution and a 6-gingerol reference substance solution; the method further comprises at least one of the steps S3 and S4; s3, dripping the test solution and the cinnamaldehyde reference solution on the same thin-layer plate, developing with a first developing solvent, and identifying the cinnamon; the first developing solvent comprises petroleum ether and ethyl acetate in a volume ratio of (15-20): 3; s4, dripping the test solution and the 6-gingerol reference substance solution on the same thin-layer plate, developing with a second developing solvent, and identifying the ginger; the second developing solvent is prepared from petroleum ether, trichloromethane and ethyl acetate according to the volume ratio of (2-4): (1-2): (1-3). The method is good in overall separation effect, clear in spots, good in specificity and durability, and capable of identifying the medicine flavors of cinnamon and ginger in the vitality-preserving decoction and the preparation thereof.

The invention discloses a thin-layer chromatography identification method for cinnamon and ginger in vitality-preserving granules, and belongs to the technical field of pharmaceutical analysis. Aiming at the problems of step-by-step detection, serious cinnamic acid tailing and dependence on toxic solvents in the existing method, the method comprises the following steps: dissolving vitality-preserving particles in water, carrying out ultrasonic treatment, extracting filtrate by using absolute ether, merging extract liquor, evaporating to dryness, and dissolving by adding ethyl acetate to obtain a test solution; preparing a cinnamon reference medicinal material solution and a cinnamic acid and 6-gingerol reference substance solution; a silica gel GF254 thin-layer plate is adopted, petroleum ether-normal hexane-ethyl formate-formic acid is used as a developing solvent for developing, and inspection is conducted under the ultraviolet light of 254 nm and the ultraviolet light of 365 nm respectively. Cinnamon and ginger can be synchronously identified, spots are clear and free of trailing, no toxic solvent exists, durability is high, cost is low, and an accurate and reliable basis can be provided for quality control of the vitality-preserving granules.

The invention discloses a heart-protecting granule fingerprint spectrum construction and component quantitative detection method based on HPLC (High Performance Liquid Chromatography), and belongs to the technical field of pharmaceutical analysis. The method comprises the following steps: preparing heart-protecting granules into a test solution by adopting a high performance liquid chromatography (HPLC) technology, affiliating and identifying chromatographic peaks in the test solution by taking chromatograms of a single medicinal material decoction piece solution and a standard solution as a contrast, and constructing an HPLC fingerprint spectrum containing 12 common characteristic peaks. And a foundation is laid for the establishment of a comprehensive quality control and quality evaluation method of the heart-protecting granules. The method disclosed by the invention can also be used for quantitatively detecting important components, namely naringin and salvianolic acid B, in the heart-protecting granules, has the advantages of high precision, good reproducibility, good stability, high recovery rate, good durability and accurate determination result, and provides technical support for high-precision quality monitoring and evaluation of the heart-protecting granules.

The invention relates to the technical field of pharmaceutical analysis, and discloses a method for monitoring a baicalein-betaine eutectic process based on terahertz spectroscopy, and the method comprises the following steps: mixing and ball-milling baicalein and betaine to obtain powder samples, collecting the powder samples with different ball-milling times, drying, mixing with a dispersing agent, tabletting, and drying to obtain the baicalein-betaine eutectic crystal. The method comprises the following steps: measuring spectral data by using terahertz time-domain spectroscopy equipment, selecting a characteristic peak and tracking intensity change to obtain a curve, carrying out nonlinear fitting on the curve, obtaining a molecular vibration mode through a density functional theory, and analyzing the eutectic process by combining the two modes. The terahertz spectrum technology is combined with theoretical calculation, complete analysis of the baicalein-betaine eutectic process from macrodynamics monitoring to microcosmic molecular action mechanism analysis is achieved, and an effective means is provided for eutectic formation research and process optimization.

The invention belongs to the technical field of pharmaceutical analysis, and discloses a method for determining two potential genotoxic impurities in carbon [13C]-urea by gas chromatography. Comprising the following steps: (1) preparing a sample solution; (2) preparing a reference solution; (3) taking nitrogen as carrier gas, and detecting by adopting a medium-polarity or weak-polarity chromatographic column separation system; and (4) calculating by a peak area through an external standard method to obtain the accurate contents of methyl carbamate and ethyl carbamate in the carbon [13C]-urea. The method disclosed by the invention is simple to operate, good in specificity, linearity, precision and accuracy and high in sensitivity, and can realize accurate detection of residual potential genotoxic impurities, namely methyl carbamate and ethyl carbamate, in the carbon [13C]-urea.

The invention belongs to the technical field of pharmaceutical analysis, and particularly relates to a method for separating and determining nafamostat mesylate SM2 and related impurities thereof. The method is a high performance liquid chromatography, and comprises the following steps: by taking octadecylsilane chemically bonded silica as a chromatographic column filler, a potassium hexafluorophosphate solution as a mobile phase A and acetonitrile as a mobile phase B, separating nafamostat mesylate SM2 and related impurities thereof through linear gradient elution; and detecting by using detection wavelengths of 200 nm to 240 nm and 250 nm to 280 nm to obtain a chromatogram, so that effective determination of the nafamostat mesylate SM2 and the related impurities thereof is realized. The method provided by the invention adopts common buffer salt as a mobile phase, and has the characteristics of short analysis time, strong specificity, high sensitivity and good reproducibility.

The invention relates to the technical field of drug analysis and detection, in particular to a method for detecting related substances in moxifloxacin hydrochloride bulk drugs. According to the detection method provided by the invention, the separation efficiency of the known impurity (impurity F) can be improved, and the separation degree between the main component and the adjacent impurity can reach 1.5 or above; in addition, a solvent formula is optimized, so that a test sample is more stable, and the detection method is more accurate; the detection accuracy is improved, and the recovery rate is controlled within the range of 93-102%.

The invention relates to the technical field of pharmaceutical analysis, and particularly discloses a high performance liquid chromatography-mass spectrometry tandem method (HPLC-MS / MS) for detecting a genotoxic impurity L-alanine isopropyl ester in an emtricitabine-propofol tenofovir tablet. By optimizing chromatographic column selection, a mobile phase gradient elution procedure and mass spectrometric detection parameters, the method realizes exclusive, sensitive and accurate detection of the L-isopropyl alanine. The methodological verification result shows that the detection limit is 1.00 ng / mL, the quantification limit is 2.00 ng / mL, the linear range is 2.00-100.00 ng / mL (the correlation coefficient r is equal to 0.9994), the recovery rate is stabilized between 92% and 98%, and the precision and repeatability are good. The detection method has the advantages of high sensitivity and strong selectivity, is suitable for quality control of emtricitabine propofol tenofovir tablets, meets the control requirements of ICH M7 guide on genotoxic impurities, and has good practicability and popularization value.

The invention relates to the technical field of pharmaceutical analysis, in particular to a method for detecting the content of guanidine nitrate by precolumn derivatization-high performance liquid chromatography, which comprises the following steps: oscillating and uniformly mixing a guanidine nitrate reference substance or sample and pure water at room temperature, adding sulfuric acid concentrated liquor in cold water bath to perform derivatization reaction, after the reaction is finished, placing at room temperature, and performing column chromatography to obtain the guanidine nitrate content. Adding liquid caustic soda for neutralization, carrying out gradient constant volume by using a mobile phase, and filtering by using a microporous filter membrane to respectively obtain a derivatization reference solution and a derivatization sample solution; and respectively injecting the derivatization reference substance solution and the derivatization sample solution into HPLC, recording peak areas, and calculating the content of guanidine nitrate in the sample by adopting an external standard method. The guanidine nitrate sample is pretreated through the precolumn derivatization reaction, the guanidine nitrate derivative generated through the reaction has the advantages of being high in stability and easy to separate, ammonium nitrate and nitric acid impurities can be effectively prevented from interfering the detection result, the explosive picric acid is not adopted in the detection method, and the detection method is simple, safe and easy to implement.

The invention belongs to the technical field of pharmaceutical analysis, and particularly relates to a method for separating active components in tobacco based on liquid-phase segmented separation, SPR, affinity chromatography enrichment and mass spectrometry detection, a product and application. According to the method, liquid chromatography separation, SPR detection screening, affinity chromatography enrichment and mass spectrum identification technologies are fused, and active small molecules are screened and enriched from a complex system by virtue of high sensitivity of SPR and accuracy of mass spectrum. The method comprises the following steps: firstly, carrying out liquid chromatography segmented separation on extract components to improve SPR detection precision and affinity enrichment efficiency; by means of specific binding of Ni Sepharose FF (NTA) resin and a His tag, more target proteins are fixed, and the micromolecule enrichment effect is further enhanced; and finally, identifying the molecular structure by using mass spectrum, and locking the bioactive small molecule combined with the target protein. According to the strategy, efficient enrichment of small molecule ligands is achieved, and a new way is provided for early-stage development of small molecules in drug discovery. 6.

The invention discloses an establishment method of an HPLC (high performance liquid chromatography) characteristic chromatogram of granules for treating common cold and clearing heat and a component content test thereof, and belongs to the technical field of pharmaceutical analys.According to the research, the HPLC characteristic chromatogram of the granules for treating common cold and clearing heat is established under the same chromatographic condition by adopting a high performance liquid chromatography, and the overall internal quality in a preparation is comprehensively reflected; the method for simultaneously determining the content of amygdalin, puerarin, cimicifugin and corydalis bungeana is established, methodology verifies that the method is simple, convenient and feasible, has good stability and repeatability, and can be used for detection in production process monitoring and quality evaluation of the cold and heat clearing granules to ensure that the quality of the cold and heat clearing granules meets the standard. The scheme not only scientifically restores the overall pharmacodynamic material basis of the compound preparation, but also provides a new generalizable normal form for quality control of traditional Chinese medicines with the advantages of low cost and high efficiency, and has milestone significance for promoting industry standardization and internationalization.

The invention relates to the technical field of pharmaceutical analysis, and particularly discloses a method for detecting related substances in lodoxamide tromethamine. According to the invention, chromatographic conditions are optimized, a C18 column with hybrid particles charged on the surface is used as a chromatographic column, gradient elution is carried out under a specific mobile phase, and a high performance liquid chromatography capable of effectively separating lodoxamide tromethamine from nine related substances is provided, and the related substances comprise INT1, INT2, LODT-A, LODT-B, LODT-C, LODT-D, LODT-E, LODT-F and LODT-G. Through methodological research and verification of specificity, sensitivity and the like, the detection method for the impurities in lodoxamide tromethamine provided by the invention is high in sensitivity and accuracy, and relatively good in reproducibility and durability, each impurity peak does not interfere with a main peak, each chromatographic peak can be well separated, and the detection method is suitable for large-scale industrial production. And a reliable guarantee is provided for better controlling the quality of the lodoxamide tromethamine product.

The invention relates to the technical field of pharmaceutical analysis, in particular to a method for detecting ziyuglycoside II in an ovateleaf holly bark medicinal material and application of the method. Comprising the following steps: preparation of a reference substance solution, preparation of a test solution, liquid chromatography detection and data analysis, and the data analysis comprises calculation of the content of ziyuglycoside II in the ovateleaf holly bark medicinal material. Wherein the liquid chromatography conditions are as follows: a chromatographic column filler is octadecylsilane chemically bonded silica; the mobile phase is acetonitrile (A) and 0.1% phosphoric acid aqueous solution (B), and 38%-40% A isocratic elution is adopted, or 0-10 min, 35%-40% A; in 10-45 min, 40%-46% A gradient elution is carried out. The method for detecting the content of ziyuglycoside II in the ovateleaf holly bark medicinal material is established and is applied to whole-process detection of ziyuglycoside II in the ovateleaf holly bark medicinal material and a preparation thereof, and an analysis means is provided for quality control of the ovateleaf holly bark medicinal material and the preparation thereof.

The present application relates to the field of pharmaceutical analysis, and particularly relates to a method for determining the content of cetirizine hydrochloride and preservatives in cetirizine hydrochloride oral solution. The method provided by the present application uses octadecyl bonded silica as the filler of the chromatographic column, uses a mixed solution with specific composition and proportion as the mobile phase, adopts isocratic elution, uses a content volumetric pipette to prepare the test sample solution, injects the test sample solution and the mixed control solution into the liquid chromatograph respectively to perform liquid chromatography, thereby obtaining the chromatogram of the test sample solution and the chromatogram of the mixed control solution respectively; and then according to the external standard method, the content of cetirizine hydrochloride, the content of the hydroxy methylbenzoate preservative and the content of the hydroxy propylbenzoate preservative in the cetirizine hydrochloride oral solution are calculated by the peak area. The method has strong specificity, high accuracy, good precision, linear relationship and stability, and is conducive to the evaluation of sample quality.

The invention relates to a reagent and a method for separation and identification of ofloxacin isomers, and relates to the technical field of analysis and test technologies and drug analysis, and the method comprises the following steps: mixing an isomer R / S ofloxacin sample with the reagent for separation and identification of ofloxacin isomers to prepare a mixed solution of R / S-OFLX-Vanco or R / S-OFLX-GM-metal ions; introducing the mixed solution into an ion source to generate compound ions, wherein the compound ions are monovalent positive ions of R / S-OFLX-Vanco or R / S-OFLX-GM-metal ions; and controlling the experimental device to measure the ion mobility spectrometry of the compound ions so as to obtain the enantiomeric structure information of R / S ofloxacin. The method has the effect of efficiently separating and quantitatively analyzing the enantiomer.

The invention belongs to the technical field of pharmaceutical analysis, and particularly relates to a method for determining a residual solvent in LXH-1211 by adopting headspace gas chromatography. Preparing a reference solution and a test solution of the LXH-1211, respectively carrying out headspace equilibrium on the blank solution, the reference solution and the test solution, injecting the solutions into a gas chromatograph in a headspace sample injection manner, recording a chromatogram, and calculating the content of the residual solvent in the test solution by peak area according to an external standard method; wherein the residual solvent is one or more of methanol, ethanol, dichloromethane, dioxane, N, N-diisopropylethylamine or N, N-dimethylformamide. The method is high in sensitivity, good in repeatability and high in accuracy, and can be used for simultaneously detecting the contents of various residual solvents in the LXH-1211, so that the quality of the LXH-1211 is ensured, and the safety of clinical medication is improved.

This invention belongs to the field of pharmaceutical analysis, specifically relating to a method for constructing a fingerprint spectrum of Shenfushu granules. The method for constructing a fingerprint spectrum of Shenfushu granules provided by this invention features high accuracy, high stability, good repeatability, good specificity, and strong specificity. The fingerprint spectrum analysis method for Shenfushu granules, a traditional Chinese medicine preparation for treating chronic renal failure, established by this invention can not only qualitatively analyze and identify the seven chemical components contained in this preparation, but also simultaneously present the characteristic peaks of five traditional Chinese medicines in the prescription, achieving the goal of overall quality control of multiple medicinal ingredients.

The invention relates to the technical field of pharmaceutical analysis, in particular to a method for determining the content of a sodium picosulfate oral solution. According to the method, high performance liquid chromatography is adopted for detection, a chromatographic column with octadecylsilane chemically bonded silica as a filler is selected, a phosphate buffer solution (10.9 g of monopotassium phosphate is taken, 1000ml of water is added to dissolve the monopotassium phosphate, and the pH value is adjusted to 3.5 with phosphoric acid) is taken as a mobile phase A, methanol is taken as a mobile phase B, and gradient elution is carried out. By adopting the method, the content of the sodium picosulfate oral solution can be effectively detected, the method has the advantages of strong specificity, good reproducibility, simplicity and convenience in operation and the like, and the quality of the sodium picosulfate oral solution can be effectively guaranteed.

The invention relates to the technical field of pharmaceutical analysis, and particularly discloses a method for simultaneously determining the content of benzyl alcohol and ethyl oleate. The method for simultaneously measuring the content of benzyl alcohol and ethyl oleate comprises the following steps: S1, mixing a sample to be measured with a low alcohol aqueous solution, freezing, adding non-polar straight-chain alkane, extracting and layering to obtain a liquid to be measured; s2, an Agilent DB-WAX chromatographic column is adopted and matched with optimized chromatographic conditions such as a heating procedure, gas chromatography detection is conducted on the to-be-detected liquid, and therefore simultaneous quantitative determination of benzyl alcohol and ethyl oleate is achieved. The method provided by the invention has the advantages of simplicity and convenience in operation, high accuracy, good repeatability, strong applicability and the like, and solves the problems of poor separation degree of benzyl alcohol and ethyl oleate and low detection efficiency in the prior art.

The application provides a method for detecting impurities in leboregen starting material LBS5, and belongs to the field of pharmaceutical analysis chemistry. S ,5 R )‑1‑(3‑fluorophenyl)‑3‑oxabicyclo[3.1.0]hexan‑2‑one. The method adopts high performance liquid chromatography, can simultaneously and accurately quantify one degradation impurity and four process impurities in LBS5, and has strong specificity, high sensitivity, good accuracy, good reproducibility and stable and reliable method. The method can realize the control of the impurities, ensures the safety of LBS5 as a pharmaceutical intermediate, further ensures the safety of a preparation of LBS5, reduces the risk of drug use, simultaneously reduces the difficulty of subsequent synthesis steps, realizes rapid, economic and environmental protection production. The method is suitable for laboratory research and development, and is also suitable for quality control in commercial production.

The invention relates to a qualitative and quantitative method for detecting active ingredients of root-strengthening and cough-relieving granules through UPLC-MS / MS, and belongs to the technical field of medicine analysis and detection. The four active ingredients of liquiritigenin, isoliquiritigenin, luteolin and bakuchiol in a sample can be detected at the same time. The method comprises the following steps: pretreating a sample by a 70% methanol ultrasonic extraction method to obtain a target sample solution, and detecting by using an SCIEX ExionLC AE high-pressure liquid phase system and an AB SCIEX 4500 mass spectrometer in combination with an MRM mode. Carrying out gradient elution on a liquid phase by using an HSS T3 C18 chromatographic column and 0.1% formic acid water-acetonitrile as a mobile phase; the mass spectrum is electrospray negative ion scanning, and a specific ion source and MRM parameters are set. The method can rapidly separate the four components, is short in detection period, high in sensitivity and strong in matrix interference resistance, can be accurately qualitative and quantitative, and provides support for preparation quality evaluation, pharmacodynamic mechanism research and clinical reasonable medication.

The invention relates to the technical field of pharmaceutical analysis, in particular to a chromatographic method for determining optical isomers in a carbidopa raw material medicine and a preparation thereof by using an HPLC (High Performance Liquid Chromatography) method. The detection method is simple, convenient, rapid and accurate to operate, and specifically comprises the following steps: dissolving a proper amount of a sample with a solvent, diluting to a certain concentration, taking a certain volume of the sample, carrying out isocratic elution at a certain column temperature by adopting a high performance liquid chromatography, using a hand-type chromatographic column, a mobile phase A and a mobile phase B, and carrying out quantitative analysis by adopting a self-control method with correction factors. And calculating the isomer in the test solution. The method is good in specificity, solution stability, linearity, repeatability, intermediate precision and durability, and the detection result is accurate and reliable through verification.

A method for maintaining a gene description dataset associated with a plurality of genes with respect to a given disease, wherein the gene description dataset is a human- comprehensible text-based dataset generated by a Large Language Model (LLM); maintaining a dataset of drug mechanisms of action (MOAs) associated with a plurality of drugs; embedding the gene descriptions and the MOAs into high-dimensional vectors using a text embedding technique to generate gene description vectors and MOA vectors; calculating distances between the gene description vectors and the MOA vectors to generate distance scores; and ranking the drugs for the given disease based on an aggregated distance score between the MOA vectors and the vectors of genes associated with the given disease.

The invention discloses a fingerprint spectrum of Huanglong cough-relieving granules and a construction method, and relates to the technical field of pharmaceutical analysis. The construction method comprises the following steps: (1) preparation of a test solution: taking Huanglong cough-relieving granules, and adding an extraction solvent for extraction; (2) preparing a reference substance solution; (3) preparing a reference medicinal material solution; (4) chromatographic conditions: octadecylsilane chemically bonded silica is used as a filler; the mobile phase A is acetonitrile, the mobile phase B is 0.1-0.2% phosphoric acid, the flow rate is 0.38-0.42 ml / min, the column temperature is 29-31 DEG C, the detection wavelength is 210 nm in 0-17 min, and the detection wavelength is 270 nm in 17-48 min; and (5) absorbing the solution, and adding the solution into a liquid chromatograph for detection. The fingerprint spectrum constructed by the method can systematically reflect the material basis, is relatively good in precision, repeatability and stability, and can be used for quality control of the Huanglong cough-relieving granules.

The present application relates to the technical field of pharmaceutical analysis, and in particular to a combined analysis method for exploring the effect of Huangkui capsules on improving chronic kidney injury of tacrolimus based on metabolomics and proteomics. The combined analysis method of the present application explores how Huangkui capsules improve the mechanism of chronic kidney injury of tacrolimus from multiple dimensions, which is beneficial to in-depth analysis of the specific effects of Huangkui capsules at the molecular, cellular and tissue levels, reveals the key path and regulation mechanism of kidney protection, and can also provide a scientific basis for optimizing clinical application, further promoting the modernization of traditional Chinese medicine and its wide application in the treatment of kidney disease.