Method for detecting entrapment rate of protein or polypeptide drugs in lipid vesicles

A technology of lipid vesicles and encapsulation efficiency, which is applied in the direction of measuring devices, biological tests, material inspection products, etc., to achieve the effect of strong light absorption
CN106501525AInactive Publication Date: 2017-03-15JIANGSU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGSU UNIV
Publication Date
2017-03-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a for detecting the entrapment rate of protein or polypeptide drugs in lipid vesicles, and belongs to the technical field of drug analysis. The method comprises the following steps: diluting a prepared protein or polypeptide wrapped lipid vesicle solution, taking the diluted lipid vesicle solution, adding a nonionic surfactant to carry out demulsification, taking the lipid vesicle solution before and after the demulsification, respectively adding a BCA reaction solution, and carrying out color development at a certain temperature and time; and reading the absorbance at 562 nm, and calculating the protein concentration and the entrapment rate according to a standard curve. The method uses a difference between the reaction rate of free proteins outside the lipid vesicles with a color developer and the reaction rate of the wrapped proteins in the lipid vesicles and the color developer to measure the entrapment rate of the lipid vesicle protein drugs without separating the free proteins, so the method has the advantages of convenience in operation, and good repeatability, solves the problems of tediousness and deviation in the measurement process of the entrapment rate of the lipid vesicle protein drugs, and provides an effective method and basis for relevant production application and research fields of the lipid vesicle protein drugs.
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Description

technical field

[0001] The invention relates to a method for measuring the encapsulation rate of protein or polypeptide drugs in lipid vesicles, belonging to the technical field of drug analysis. Background technique

[0002] The research on lipid vesicles of peptide and protein drugs is a very active field at present. Lipid vesicles are closed vesicles composed of lipids with a water phase inside, and hydrophilic and hydrophobic substances can be encapsulated in the water phase and membrane, respectively. According to different compositions, lipid vesicles include liposomes, transfersomes, plastids, ethosomes, etc., but their structures are all spherical closed vesicles. After oral administration, protein will be degraded and inactivated by acid and alkali conditions in the gastrointestinal tract, body fluids and enzyme damage. After oral administration of lipid vesicles as the carrier, due to the protective effect of the lipid bilayer, it can reduce the degree of degrada...

Claims

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