38results about How to "Good linear relationship" patented technology

The application belongs to the technical field of fluorescent probe detection, and discloses a bimetallic nanocluster fluorescent probe compound and application thereof in preparation of a product for rapidly detecting acetylene. The fluorescent probe compound is a gold-copper bimetallic nanocluster solution modified by adamantane thiol, which is used as a fluorescent probe for acetylene detection. The solution has a clear and controllable preparation process. Through specific proportioning and reaction conditions of cuprous chloride, adamantane thiol, tetrachloroauric acid, sodium borohydride and other reagents, a probe solution with stable fluorescence characteristics can be prepared. The bimetallic nanocluster fluorescent probe compound is applied in preparation of a product for rapidly detecting acetylene. Through specific combination of fluorescent molecules and acetylene gas to trigger the secondary effect of fluorescence quenching, detection is realized, and other substances cannot trigger quenching. At a wavelength of 700 nm, the linear relationship (R 2 >0.99) between the fluorescence intensity and the acetylene concentration realizes accurate quantification of the acetylene concentration, and the minimum detection limit is 0.026 muL / L.

This invention relates to the field of electroplating solution analysis technology, and in particular to a rapid analytical method for polyethylene glycol (PEG) additives in copper electrodeposition solutions. The method involves protonated PEG forming a macromolecular complex colloid with phosphomolybdate under acidic conditions of pH 1.0–2.0. This colloid exhibits maximum absorption at 372 nm, and the absorbance value is directly proportional to the PEG concentration in the formed complex colloid. This allows for quantitative analysis of PEG in the copper electrodeposition solution. Because the phosphomolybdate-PEG complex colloid selected in this invention has excellent selectivity, and no sample pretreatment is required, this invention offers advantages such as ease of operation, speed, high sensitivity, and good selectivity. It is particularly suitable for intermediate control analysis in production environments, such as for electroplated copper interconnects in chips and printed circuit boards, as well as electrolytic copper foil for negative electrode current collectors and copper-clad laminates in energy storage batteries.

The invention discloses a dual-mode detection kit for highly sensitive detection of ERalpha based on HRP-streptavidin. The dual-mode detection kit comprises the following raw materials: a glassy carbon electrode, 1-AP-CNHs, AuNPs, Sh-Apt, MCH, Bio-Ab, and HRP-streptavidin. The invention successfully constructs an electrochemical detection kit for detecting ERalpha with high sensitivity on the basis of the 1-AP-CNHs / AuNPs composite material. According to the invention, due to the redox activity of HRP-streptavidin, the HRP-streptavidin and H2O2 are subjected to redox reaction to generate a current signal; in addition, hydrogen peroxide can be catalyzed to generate. OH free radicals, and the free radicals can oxidize TMB to generate blue products, so that colorimetric reaction is initiated, and ERalpha can be detected more accurately. The electrochemical-colorimetric dual mode of the 3D 1-AP-CNHs / AuNPs composite material and the HRP-streptavidin for high-sensitivity detection of the ERalpha disclosed by the invention greatly improves the detection accuracy. The kit has the advantages of good selectivity, strong stability, high sensitivity, anti-interference performance, environmental protection and the like.

This invention discloses hollow silver-platinum nanoalloy particles, their preparation method, and applications. The silver-platinum nanoalloy particles have a particle size of 30-60 nm, an overall hollow structure, and a porous surface distribution of silver and platinum fused together. The preparation method includes: using epigallocatechin gallate as the sole reducing agent and stabilizer, reducing silver nitrate in ethylene glycol to prepare monodisperse silver nanoparticles; then, at 60-80°C, slowly adding chloroplatinic acid solution in batches to the silver nanoparticle solution to carry out an in-situ exchange reaction; after the reaction, washing and separation yields the final product. This invention provides a green, simple, mild, and highly controllable preparation method. The prepared silver-platinum nanoalloy particles simultaneously possess excellent nanozyme catalytic activity and high-sensitivity surface-enhanced Raman scattering performance, achieving integrated synergistic catalytic and detection functions. It has broad application prospects in food safety testing, environmental monitoring, and biosensing.

The application relates to the field of polyamide material analysis, and discloses application of an indantrione color developing reagent in determination of polyamide hydrolysis products and a method for determining the content of the polyamide hydrolysis products, wherein the indantrione color developing reagent contains an indantrione-ethylene glycol solution and an acetic acid-sodium acetate buffer solution. The application provided in the application adopts ethylene glycol as a single solvent of the indantrione, effectively inhibits oxidative degradation of the indantrione under high-temperature color developing conditions, and enables uniform color development of oligomers. The application does not add antioxidants, avoids interference with color developing reactions, is accurate and reliable in determination, is simple in preparation due to the single solvent, has a long storage time under light shielding at 4 DEG C, and can not only accurately determine monomers of polyamides and dimers to heptamers of the monomers, but also accurately determine various polyamide hydrolysis products, and has a good linear relationship.

This invention provides a method for detecting tetrachloronaphthalene, a persistent organic pollutant in eel products. The specific steps include: eel sample preparation, target analyte extraction and matrix purification, followed by analysis using ultra-high performance liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry (UPLC-APCI-MS / MS). The measured peak area, fractionation ratio, and other relevant parameters are substituted into a specific formula to calculate the measured value of tetrachloronaphthalene in the test solution. This method fills the gap in my country's technology for detecting tetrachloronaphthalene-like persistent organic pollutants in eel products. It effectively eliminates interference from the complex matrix of eel products, accurately determines four tetrachloronaphthalene isomers in trace amounts, and possesses significant advantages such as high sensitivity, strong anti-interference ability, simple operation, and high detection accuracy.

This invention discloses a peroxynitroso anion fluorescent probe with AIE effect, its synthesis method, and its applications. The near-infrared fluorescent probe TPS was designed and synthesized in this invention. It is nearly colorless under ultraviolet light irradiation but emits bright yellow fluorescence upon reaction with peroxynitroso anions. Detection showed that TPS reacts with ONOO in a HEPES:acetonitrile = 1:1 system. ‑ The response time was 100 min, after adding 120 uL of 0.5×10 ‑3 mol·L ‑1 ONOO ‑ The reaction is complete. TPS exhibits high specificity, reacting only with peroxynitrosoanion among various anions, demonstrating high sensitivity. TPS reacts with ONOO at pH 4-8. ‑ The ion response exhibits good stability, and TPS is effective against ONOO. ‑ The response exhibits good linearity, with a detection limit of 0.08232. µmol·L ‑1 Probe TPS and ONOO ‑ The reaction, after different concentrations of THF, exhibited a typical AIE effect.

The application belongs to the field of chemical detection, and particularly relates to a method for detecting ethylene oxide, 2-chloroethanol and acetaldehyde in frozen drinks by solid phase microextraction gas chromatography mass spectrometry. The method for detecting ethylene oxide, 2-chloroethanol and acetaldehyde in frozen drinks by solid phase microextraction gas chromatography mass spectrometry comprises the following steps: S.1) taking a frozen drink sample to be detected, and uniformly mixing; S.2) weighing a certain amount of the sample, and adding sodium chloride and hydrochloric acid; S.3) determining the sample by solid phase microextraction gas chromatography mass spectrometry; and S.4) quantitatively analyzing the content of ethylene oxide, 2-chloroethanol and acetaldehyde in the frozen drink sample to be detected by an external standard method. The application combines a derivatization reaction and a solid phase microextraction process into one, and overcomes the difficulties in traditional analysis technology, such as complex pretreatment, long time consumption, low accuracy, unstable results, high detection limit and the like. Meanwhile, the detection result has high precision, the influence of a complex matrix is removed, and the reproducibility is good.

The invention discloses a pulsed eddy current thickness evaluation method based on symmetric entropy. Comprising the following steps: establishing a three-layer pulsed eddy current analysis simulation model of air in a pipeline, a pipeline wall and a coating layer; performing differential normalization on the detection signal to obtain a preprocessed signal; equally dividing the pre-processed signal into N discrete event intervals according to a time sequence, calculating local energy of the pre-processed signal and a mirror image signal in each interval, and normalizing the local energy into two groups of probability density functions; constructing a symmetric entropy characteristic quantity based on the Shannon information entropy so as to represent signal energy distribution symmetry change; symmetrical entropies of different known thicknesses are collected, and a quantitative mapping model between the symmetrical entropies and the pipeline thicknesses is established; and substituting the symmetric entropy value of the detection signal into the fitting curve to realize inversion and quantitative evaluation of the pipeline thickness. The pulsed eddy current thickness evaluation method provided by the invention shows good linear correlation in thickness quantification and shows strong robustness under lift-off interference.

The invention relates to the technical field of treatment drug monitoring, in particular to a method for simultaneously determining the concentration of multiple antiepileptic drugs in plasma. Comprising the following steps: S1, preparing a standard working solution; s2, preparing an internal standard solution; s3, mixing the standard working solution with blank plasma, adding an internal standard solution, centrifuging, and detecting to obtain a chromatogram of a standard solution; s4, based on the chromatogram, taking the ratio of the peak area of the anti-epileptic drug to the peak area of the corresponding internal standard substance as a vertical coordinate Y, taking the ratio of the concentration of the anti-epileptic drug to the concentration of the corresponding internal standard substance as a horizontal coordinate X, and fitting to obtain a standard curve equation; s5, preparing a plurality of groups of quality control working solutions, and verifying the standard curve equation obtained in the step S4; and S6, uniformly mixing the blood to be detected with the internal standard solution, centrifuging, detecting the supernate, and calculating the concentration of the antiepileptic drug in the plasma. The method can realize simultaneous, accurate and quantitative detection of multiple antiepileptic drugs in blood.

The invention discloses a direct mass spectrometry device and method for trace uranium in a water body, and relates to the technical field of analys.The method comprises the steps that a gel film is soaked in uranium-containing wastewater and taken out, a tip is cut out, and the uranium-adsorbed gel film is obtained; dropwise adding an extracting agent on the gel film, and then applying high voltage on the gel film, so that uranium adsorbed on the gel film is extracted and moves towards the tip along with the solvent to form spray; uranium is ionized in the form of uranyl acetate and detected by a mass spectrum detection system; according to the invention, accurate analysis of trace uranium can be realized, and mass loss of uranium in a water sample due to links such as sample treatment and preservation is avoided. The device can be applied to various complex water environments, and direct mass spectrometry can be realized only by shearing gel into a triangular shape with a tip and applying voltage and an extracting agent during analysis. And the method has the advantages of high analysis speed, good linear relationship, low detection limit and the like.

The invention discloses a metal ion functionalized NT-proBNP photoelectrochemical sensor and a preparation method, and belongs to the technical field of photoelectrochemical biosensors, the photoelectrochemical sensor comprises a Fe < 3 + >-coated BiVO4 electrode, and the preparation method of the electrode comprises the following steps: dispersing BiVO4 in deionized water, adding excessive Fe < 3 + > for adsorption, removing the excessive Fe < 3 + > after the adsorption is finished, and obtaining the metal ion functionalized NT-proBNP photoelectrochemical sensor. And then dispensing the Fe < 3 + >-coated BiVO4 on a conductive material, so as to obtain the Fe3 +-coated BiVO4 / Fe3 +-coated BiVO4 / Fe3 +-coated BiVO4 composite material. When the photoelectrochemical sensor provided by the invention is used for NT-proBNP detection, no trapping agent or bias voltage needs to be applied, measurable signal output is realized through enzyme-linked immunosorbent assay, and the detection scheme has good selectivity and stability, can realize accurate detection of NT-proBNP, and has good application prospects in clinical diagnosis.

This invention discloses a method for detecting hexavalent chromium in cement, belonging to the field of cement quality testing technology. The method includes: preparation of standard solution, preparation of colorimetric reagent, plotting of standard curve, cement sample processing, sample determination, and waste liquid treatment. This invention solves the problems of insufficient detection stability, non-standardized operation procedures, and poor environmental performance of existing technologies. It has the advantages of accurate detection, simple operation, and safety and environmental protection, and can be widely applied to cement production quality control and environmental testing scenarios.

The application belongs to the technical field of gene detection, and particularly relates to specific primers, a Taqman probe and application of a bacillus velezensis HMB26553 strain. The specific primers and the probe sequence of the bacillus velezensis HMB26553 strain are respectively SEQ ID NO. 1, SEQ ID NO. 2 and SEQ ID NO. 3. The primers can amplify a single unique target fragment from the bacillus velezensis HMB26553 strain, and realize specific detection of the bacillus velezensis HMB26553 strain. The primers have the characteristics of high specificity, high detection efficiency, low false positive rate, long preservation time and the like. By using the primers and through real-time quantitative PCR technology, the population quantity of the bacillus velezensis HMB26553 strain in the rhizosphere of cotton can be quickly quantified, and the primers have the advantages of high throughput, rapidness and accuracy.

The invention discloses an AFB1 colorimetric-electrochemical dual-mode detection kit based on MIL-88 (at) Pd / Pt nano-enzyme and application of the AFB1 colorimetric-electrochemical dual-mode detection kit. According to the invention, AFB1-Apt is connected to MBs through an amidation reaction. And the MIL-88 (at) Pd / Pt is connected to the cDNA through an amide reaction. And then, through DNA hybridization, the cDNA and AFB1-Apt are complementarily combined. In the presence of AFB1, it specifically binds to AFB1-Apt, resulting in double strand hybridization dissociation. After magnetic separation, NaAc-HAc, TMB and H2O2 are added to precipitate, colorless TMB is catalyzed to be oxidized into blue oxTMB through a reaction system, a colorimetric signal is generated, and the absorbance change of the oxTMB is measured. The supernate is added to the treated GCE, and the electrode is immersed in a PBS buffer solution containing H2O2. The electrochemical reduction of H2O2 generates a significant SWV signal. According to the invention, a high-performance dual-mode sensing platform is constructed, cooperative amplification and cross validation of signals are realized, and the method has the advantages of high selectivity, high sensitivity, low cost and the like.

The application provides a gas chamber type three-dimensional contact force sensor, which is characterized in that a plurality of air pressure sensors are arranged in closed soft air chambers on a bottom plate of the gas chamber type three-dimensional contact force sensor; the soft air chambers are formed by surrounding elastic contact body shells (4) arranged on the bottom plate and located above or beside the air pressure sensors; when the elastic contact body shells are deformed under the action of external three-dimensional force, the soft air chambers of the air pressure sensors at the force receiving positions are deformed and the air pressure in the soft air chambers is changed, the gas chamber type three-dimensional contact force sensor processes the air pressure data of the soft air chambers measured by the air pressure sensors to obtain external three-dimensional force information; the application has three-dimensional force detection capability, simple preparation process, low manufacturing cost, low requirement for the operation environment and is suitable for mass production.

This invention belongs to the field of chemical detection technology, specifically relating to a method for detecting diethylstilbestrol (DES) based on a photothermal probe and lateral chromatography test strip. The method comprises a photothermal material coupled with a DES monoclonal antibody. The detection zone of the lateral chromatography test strip is coated with a PBS solution containing DES antigen, while the control zone is coated with a PBS solution containing goat anti-mouse secondary antibody. This invention combines the photothermal properties of the photothermal material with lateral chromatography test strip technology to achieve sensitive quantitative analysis of DES. In this invention, an 808nm laser is used to irradiate the control and detection zones of the lateral chromatography test strip, and a thermal imager is used to record the temperature changes in each zone. A standard curve is established using the temperature difference and DES concentration to achieve quantitative analysis of DES. This invention can be used for rapid, accurate, intuitive, highly repeatable, and highly stable quantitative detection of samples containing DES.

The invention discloses a near-infrared fluorescent probe for polystyrene nano-plastic detection and a preparation method and application thereof, the molecular formula of the fluorescent probe is C19H20NO2, and the fluorescent probe is generated by taking 2 '-hydroxyacetophenone and 4-dimethyl amino cinnamyl aldehyde as raw materials through an aldol condensation reaction under base catalysis. Based on a twisted intramolecular charge transfer mechanism, the probe is specifically combined with a polystyrene (PS) nano-plastic surface through hydrophobic interaction, intramolecular rotation is inhibited, a fluorescence signal is recovered, a good linear relation (R = 0.9913) is shown in the concentration range of 0-90 mu g / mL, and the detection limit is as low as 36.4 ng / mL. Meanwhile, the probe has the advantages of excellent anti-interference capability and biocompatibility, multi-scale detection from the environment to a biological system is realized, especially in-situ imaging capability at the levels of cells, plants and animals, and an integrated solution is provided for research on environmental behaviors and health effects of nano-plastics.

The application belongs to the technical field of medicine detection, and particularly relates to a detection method of impurities in 2-(3-formyl-4-hydroxyphenyl)-4-methylthiazole-5-carboxylic acid ethyl ester. The method uses high performance liquid chromatography to detect related substances in 2-(3-formyl-4-hydroxyphenyl)-4-methylthiazole-5-carboxylic acid ethyl ester, and the content of each impurity is calculated by a principal component self-control method with a correction factor. Methodology verification shows that the detection method provided by the application is good in specificity, high in accuracy and sensitivity, and can be used for quality control of related substances in 2-(3-formyl-4-hydroxyphenyl)-4-methylthiazole-5-carboxylic acid ethyl ester, so that the product quality can be effectively controlled, and the medication safety of febuxostat medicine is improved.

The application discloses a flow measuring device and method based on micro-channel groove inner vortex flow, and belongs to the flow measuring device field. In the measuring device, the fluid main channel is of a rectangular structure, the circular micro-groove is of an arc structure formed by the intersection of the circular shape and the fluid main channel, the circular micro-groove is communicated with the fluid main channel at the intersection position, and a particle and a second-phase insoluble liquid supplement channel is arranged to input the second-phase insoluble liquid and single tracer particles into the circular micro-groove; the fluid main channel is communicated with the to-be-measured fluid, the to-be-measured fluid drives the tracer particles to form secondary flow, and a micro light sensing sensor detects the motion frequency of the tracer particles to calculate the flow of the to-be-measured fluid. Through the technical scheme of the application, the detection of different fluid flows is realized, has considerable resolution, is suitable for the particle tracking speed measurement without pollution for the to-be-measured sample which is not suitable for the tracer particles, the device structure is simple, easy to manufacture, compact and portable, and is suitable for large-scale production.

The invention discloses a PEDV (Porcine Epidemic Diarrhea Virus) sIgA detection reagent, a PEDV sIgA detection method and application. The reagent comprises a first probe and a second probe. The first probe comprises a first binding element and a first fluorescent reporter molecule which are coupled with each other, and the first binding element comprises a PEDV S2 protein capable of being combined with a variable region of PEDV sIgA or a stabilizing mutant of a functional fragment of the PEDV S2 protein. The second probe comprises a second binding element and a second fluorescence reporter molecule which are coupled with each other, and the second binding element comprises an anti-porcine IgA antibody capable of being bound with a PEDV sIgA constant region. The first fluorescent reporter molecule and the second fluorescent reporter molecule form a fluorescence resonance energy transfer (FRET) pair. Compared with the prior art, the detection reagent provided by the invention can realize rapid, homogeneous and high-throughput quantitative detection of PEDV sIgA, and the detection sensitivity, the specificity and the signal-to-noise ratio of the detection reagent are greatly improved.

The present application relates to the field of electrochemical analysis and detection, and discloses a method for detecting mycotoxin based on CRISPR-Cas12a system, comprising: (1) reacting single-stranded DNA with recognition DNA of mycotoxin to obtain single-stranded-recognition DNA complex, wherein the single-stranded DNA is complementary to the recognition DNA; (2) mixing the single-stranded-recognition DNA complex and the sample to be tested which may contain mycotoxin to obtain reaction mixture I after reaction II, and mixing the reaction mixture I with auxiliary DNA containing activated DNA fragments to obtain reaction mixture II after reaction III, wherein the auxiliary DNA is complementary to the single-stranded DNA; (3) mixing the reaction mixture II with CRISPR-Cas12a system solution to obtain reaction solution after activation; (4) adding the reaction solution dropwise to an electrochemical biosensor to perform reaction V, and detecting the electrochemical signal of the electrochemical biosensor after reaction V is completed. The method has high sensitivity and high selectivity, and is simple and rapid to operate.

This invention discloses a method for measuring the activity of radioactive polonium aerosol in a lead-bismuth reactor, belonging to the field of radioactive material measurement technology. 210 Po solution was used to prepare test samples with different activity ranges and different collection carriers, and the activity was measured using an energy dispersive spectrometer. A correlation between the measured activity and the theoretical activity was then established to calibrate the measured activity of actual samples. This invention employs the above-mentioned method for measuring the activity of radioactive polonium aerosol in a lead-bismuth reactor, by flexibly establishing a correlation between the measured activity and the theoretical activity within a certain activity range. 210 The linear correlation between the theoretical activity and the measured activity of the Po standard solution enables immediate measurement without disturbing the original sample, greatly ensuring the accuracy of the measurement. This is of great significance for timely and effective protection in lead-bismuth reactors. At the same time, this method can also be applied to other environments to quickly and accurately obtain the true activity of polonium-containing samples.

The present application relates to the technical field of pharmaceutical analysis, and in particular to a method for detecting active ingredients in a traditional Chinese medicine preparation containing Concha Murex. The present application first establishes a method for simultaneously detecting aurantio-olive yellow saponin, syringidin and ammonium glycyrrhizinate. The method is simple to operate, has good specificity, solution stability, linearity, repeatability and accuracy. The method provided by the present application provides a more reliable quality control method for traditional Chinese medicine preparations containing Concha Murex, and ensures the clinical efficacy of drugs.

The application discloses a kind of graft injury multi-index combined noninvasive detection kit, by optimized multiplex real-time fluorescent quantitative polymerase chain reaction technology, realize the quantitative detection of six categories with closely related DNA virus after transplantation Complications, the present application relates to the field of in vitro detection technology of clinical sample.The graft injury multi-index combined noninvasive detection kit, the present application first realizes the synchronous detection of BK virus, JC virus, B19 virus, CMV virus, adenovirus and six kinds of closely related viruses of circovirus (TTV) with graft injury.Test data see example 7, from the detection results of 100 clinical kidney transplant patients, it can be seen that the kit of the present application can simultaneously accurately detect the load level of six viruses, to provide comprehensive infection risk assessment for clinicians.Compared with the prior art single virus detection method, the present application avoids the cumbersome process of multiple sampling and detection, significantly improves the clinical work efficiency.

The application belongs to the technical field of optical detection, and particularly relates to a spectrum detection method based on differential absorption, which can detect and analyze the absorption spectrum of most non-single-element molecules. The application is a tunable semiconductor laser absorption spectrum technology detection method based on the principle of differential absorption, which optimizes the defect of insufficient tunable ability of traditional absorption spectrum technology, can detect and analyze the absorption spectrum of most non-single-element molecules, and provides a new path for the development of tunable semiconductor laser absorption spectrum technology.

This invention belongs to the field of pharmaceutical analysis technology and relates to a method and reagent kit for detecting the dissolution rate of zinc acetate solid dosage forms. The method involves dissolving the zinc acetate solid dosage form using a dissolution medium; collecting the dissolution solution at a set time; filtering the dissolution solution to obtain a subsequent filtrate; diluting the subsequent filtrate with a 1.5-2.5% hydrochloric acid solution as a diluent to obtain a diluted solution; and detecting the zinc acetate concentration in the diluted solution using atomic absorption spectrophotometry. This detection method avoids the influence of the matrix on the sample during detection, thus enabling more stable and accurate detection of the dissolution rate of zinc acetate solid dosage forms. This method is beneficial for the development of zinc acetate tablet solid dosage forms and is of great significance for improving the quality and efficacy of zinc acetate tablets.

The invention discloses a cyanide ratio response Raman probe and a preparation method and application thereof.The probe is composed of a gold nanostar substrate and cyanide ion response Raman reporter molecules connected through gold-sulfur bonds, and the reporter molecules are connected with 1, 1, 2, 3, 4-tetracarboxylic acid through ethylene bridges. 2, 2-trimethyl-3-ethyl-1H-benzoindole and N-ethylcarbazole-3-formaldehyde are used as recognition units, and can specifically react with CN <->. The probe adopts a ratio detection strategy, takes a 1507cm <-1 > peak as an internal reference and a 2105cm <-1 > peak as a response signal, is linearly correlated with the CN <-> concentration in a range of 0.1-10 mu M (R2 is equal to 0.9902), the detection limit reaches 0.1 mu M, and the sensitivity is improved by more than 10 times compared with that of a traditional SERS (Surface Enhanced Raman Scattering) method. The probe is high in anti-interference performance, response to common anions, thiol and active oxygen can be ignored, signal change to CN <-> reaches 15.5 times, single detection only needs 3 minutes, and the probe is suitable for rapid and high-selectivity quantitative detection of cyanide in a complex environment.

This invention discloses a method for detecting budesonide content in budesonide nasal spray, relating to the field of chemical analysis technology. The steps are as follows: First, a budesonide nasal spray sample is taken, cellulase is added for enzymatic hydrolysis, then diluted with the mobile phase, shaken well, centrifuged, and filtered to obtain a test solution; then, a budesonide reference standard is taken, dissolved and diluted with the mobile phase to obtain a reference solution; the test solution and the reference solution are injected into a high-performance liquid chromatograph for detection, respectively, to obtain peaks of budesonide isomer A and budesonide isomer B; finally, the content is calculated by the sum of the peak areas of budesonide isomer A and budesonide isomer B using the external standard method. This method, by introducing a cellulase enzymatic hydrolysis step in the sample pretreatment, effectively hydrolyzes the co-treated microcrystalline cellulose and sodium carboxymethyl cellulose, achieving complete separation of the active ingredient budesonide from the excipients.

The application discloses an anti-CK18 M30 fragment recombinant rabbit monoclonal antibody pair and application thereof, relates to the field of biotechnology, and the antibody pair comprises antibody 1 and antibody 2; the heavy chain variable region amino acid sequence of the antibody 1 is shown as SEQ ID NO:1, and the light chain variable region amino acid sequence is shown as SEQ ID NO:2; the heavy chain variable region amino acid sequence of the antibody 2 is shown as SEQ ID NO:3, and the light chain variable region amino acid sequence is shown as SEQ ID NO:4; the antibody pair is obtained by mammalian cell recombinant expression; the anti-CK18 M30 fragment recombinant rabbit monoclonal antibody pair provided by the application can specifically recognize and combine CK18 fragment M30 in human serum, has high specificity and sensitivity, and the antibody pair can accurately detect the level of CK18 fragment M30 in serum.