130results about How to "Good linear relationship" patented technology

The invention discloses a non-derivatization quantitative detection method for amino acid in a high-concentration protein preparation, which comprises the following steps: separating by using a graphitized carbon chromatographic column, adding an ion pair reagent trifluoroacetic acid into a mobile phase, and detecting by using an electric fog detector (CAD); the method comprises the following steps: preparing a plurality of groups of amino acid standard substance solutions with different concentration groups, wherein the concentration range is 1mmol / L-20mmol / L; drawing a quadratic equation standard curve of the amino acid standard product concentration and the peak area as a detection equation for detecting the amino acid concentration; the method comprises the following steps: diluting a test sample by using ultrapure water for multiple times, carrying out ultrafiltration centrifugal treatment, removing protein, introducing the sample, obtaining a corresponding peak area according to the spectrum integral of the test sample, and substituting the peak area into the detection equation to obtain the concentration of amino acid in the test sample. The method has the advantages of high detection precision, high detection efficiency and simple operation, and greatly reduces the adverse effect of high-concentration protein on the chromatographic column.

The invention discloses an electrochemical detection method of dopamine, and belongs to the technical field of analysis. According to the method, SnS2 quantum dots are loaded on Zn-MOF and modified on an electrode, then gold nanoparticles and a sulfydryl-containing dopamine aptamer single chain are sequentially fixed on the electrode, an Apt / AuNPs / SnS2QDs (at) Zn-MOF / GCE composite electrode (in an open state) is formed, an electrochemical signal of the composite electrode is greatly improved, and based on specific recognition between dopamine and an aptamer chain, the electrochemical performance of the composite electrode is improved. A large cavity of Zn-MOF is used for packaging SnS2 quantum dots with excellent electrochemical properties, electrochemical signal amplification is achieved, when a target object dopamine exists, dopamine can quench an ECL signal, an electric signal is obviously reduced (in a closed state), and therefore a linear relation between the change of the electrochemical signal and the concentration of dopamine is constructed, and the electrochemical property of the electrochemical signal is improved. Therefore, high-sensitivity and high-selectivity detection of dopamine is realized.

The invention relates to the technical field of a preparation method of a determination kit for in-vitro determination of adiponectin in a human body, and particularly discloses a preparation method of a determination kit for in-vitro determination of adiponectin in a human body, which comprises the following steps: a coupling process; an S-shaped bent pipe is installed on the side connector, an upper connector is kept in an open state, a perfusion device adds 0.3-0.4 volume of a sealing buffer solution into the glass bottle, and 0.1-0.2 volume of the sealing buffer solution is injected into the glass bottle through an injection connector; and a storage process: closing the filling interface, opening the cover body, installing the up-down extending pipe, covering the cover body, opening the filling interface, carrying out resuspension ultrasonic treatment by using the storage buffer solution, and carrying out centrifugal washing for three times, thereby finally obtaining the adiponectin latex antibody. The device is easy and convenient to operate, simple in structure, independent of the manual control level and high in preparation efficiency, the situation that materials are left in a small-size glass bottle, consequently, the material ratio is difficult to control, and a small amount of confining liquid is needed is avoided, and the prepared adiponectin latex antibody is good in accuracy.

The invention relates to a fluorine-based three-dimensional covalent organic framework material as well as a preparation method and application thereof, and belongs to the technical field of covalent organic framework materials. Under the action of a catalyst, a first monomer (the first monomer is selected from a regular tetrahedron-containing amino monomer or a regular tetrahedron-containing aldehyde group monomer) and a second monomer (the second monomer is selected from a fluorine-containing aldehyde group monomer or a fluorine-containing amino monomer) are subjected to a condensation reaction in an organic solvent to obtain the fluorine-based three-dimensional covalent organic framework material. The method comprises the following steps of: preparing a solid-phase microextraction probe by taking the perfluorinated compound as an adsorbent through in-situ growth on the surface of a probe matrix, and then combining the solid-phase microextraction probe with an open-type mass spectrum to detect trace perfluorinated compounds in a complex matrix. The fluorine-based three-dimensional covalent organic framework material disclosed by the invention is good in chemical stability and high in hydrophobicity, has a good adsorption effect on perfluorinated compounds, and is high in repeated utilization rate.

The invention discloses a hydroxyproline rapid detection molecularly imprinted electrochemical sensor as well as preparation and application thereof, and relates to the field of quality safety monitoring of medicinal plants. The preparation method of the molecularly imprinted electrochemical sensor comprises the following steps: preparing the Fc-MWCNTs composite material, preparing the Fc-MWCNTs / GCE, and preparing the molecularly imprinted electrochemical sensor MIP-Fc-MWCNTs (at) GCE. According to the invention, MWCNTs with good conductivity and Fc which has good stability and oxidation-reduction property and is used as an excellent electron transfer medium are used as electrode substrate materials; and the Fc-MWCNTs and the MWCNTs are covalently bonded through an amide reaction to prepare the composite material Fc-MWCNTs which has excellent electron transfer medium characteristics, low potential reproducibility and strong stability and can remarkably improve the electron transfer rate between the electrode surface and an electrolyte. The modified composite material Fc-MWCNTs is modified on the surface of GCE, so that the molecularly imprinted electrochemical sensor MIP-Fc-MWCNTs (at) GCE with high sensitivity, high selective recognition and fast detection is constructed, and the molecularly imprinted electrochemical sensor is used for detecting an index component Hyp of cornu cervi pantotrichum.

The invention provides a crystal phase impurity analysis method, and belongs to the technical field of analytical chemistry. The method comprises the following steps: acquiring an X-ray diffraction pattern of a sodium zirconium cyclosilicate sample to be detected; according to the standard curve or standard equation of the crystal phase impurities and the X-ray diffraction pattern of the sodium zirconium cyclosilicate sample to be detected, the content of the crystal phase impurities in the sodium zirconium cyclosilicate sample to be detected is obtained; the standard curve is obtained by fitting the ratio of the mass of the crystal phase impurities to the mass of the sodium zirconium cyclosilicate in a standard curve sample and the ratio of the diffraction intensity of characteristic diffraction peaks of the crystal phase impurities to the diffraction intensity of characteristic diffraction peaks of the sodium zirconium cyclosilicate in an X-ray diffraction pattern of the standard curve sample; the standard equation is a linear equation corresponding to the standard curve; the standard curve sample is a mixture of a blank sodium zirconium silicate sample and a crystal phase impurity reference substance. By adopting the method disclosed by the invention, the content of the crystal phase impurities such as CPA and CPB in the sodium zirconium cyclosilicate can be simply, conveniently, quickly and accurately detected.

The invention discloses a method for determining the contents of ester solvents and additives in a sodium-ion battery electrolyte by GC-MS. Through preparation of a diluent, selection of a program method and control of conditions, the types and contents of the solvents and the additives in the sodium-ion battery electrolyte can be rapidly and accurately determined in one step. Therefore, the method can be widely applied to the field of component analysis of the sodium-ion battery electrolyte.

The invention relates to the technical field of cell apoptosis detection, in particular to a kit for cell apoptosis detection and a product thereof. The Annexin V-FITC provided by the invention can well realize the detection of apoptosis, has obvious linear correlation with the apoptosis rate, and can realize the high-sensitivity detection of the occurrence of apoptosis. The method and the kit for detecting cell apoptosis can effectively identify phosphatidylserine exposed on the outer surface of apoptotic cells, and have more detection advantages compared with Annexin V full length. The sensitivity and the cell apoptosis rate of the detection method have a good linear relationship, and the detection method has a relatively great application prospect.

The application belongs to the technical field of fluorescent probe detection, and discloses a bimetallic nanocluster fluorescent probe compound and application thereof in preparation of a product for rapidly detecting acetylene. The fluorescent probe compound is a gold-copper bimetallic nanocluster solution modified by adamantane thiol, which is used as a fluorescent probe for acetylene detection. The solution has a clear and controllable preparation process. Through specific proportioning and reaction conditions of cuprous chloride, adamantane thiol, tetrachloroauric acid, sodium borohydride and other reagents, a probe solution with stable fluorescence characteristics can be prepared. The bimetallic nanocluster fluorescent probe compound is applied in preparation of a product for rapidly detecting acetylene. Through specific combination of fluorescent molecules and acetylene gas to trigger the secondary effect of fluorescence quenching, detection is realized, and other substances cannot trigger quenching. At a wavelength of 700 nm, the linear relationship (R 2 >0.99) between the fluorescence intensity and the acetylene concentration realizes accurate quantification of the acetylene concentration, and the minimum detection limit is 0.026 muL / L.

The present invention discloses a method for analyzing the chemical composition and content determination of Chlorophytum comosum based on liquid chromatography-quadrupole-electrostatic field orbital trap tandem mass spectrometry, including preparing a reference solution, preparing a test solution, and chromatographic analysis, wherein the chemical composition analysis also includes mass spectrometry analysis and data processing. This application is the first to simultaneously quantitatively analyze three types of components in Chlorophytum comosum, namely flavonoids, phenylethanoid glycosides, and organic acids. The results show that the contents of these five components in medicinal materials from different origins vary greatly, which may be due to factors such as different origins, growth environments, climatic conditions, and harvesting times that affect the accumulation of compounds in plants. The specific reasons need further study.

This invention relates to the field of electroplating solution analysis technology, and in particular to a rapid analytical method for polyethylene glycol (PEG) additives in copper electrodeposition solutions. The method involves protonated PEG forming a macromolecular complex colloid with phosphomolybdate under acidic conditions of pH 1.0–2.0. This colloid exhibits maximum absorption at 372 nm, and the absorbance value is directly proportional to the PEG concentration in the formed complex colloid. This allows for quantitative analysis of PEG in the copper electrodeposition solution. Because the phosphomolybdate-PEG complex colloid selected in this invention has excellent selectivity, and no sample pretreatment is required, this invention offers advantages such as ease of operation, speed, high sensitivity, and good selectivity. It is particularly suitable for intermediate control analysis in production environments, such as for electroplated copper interconnects in chips and printed circuit boards, as well as electrolytic copper foil for negative electrode current collectors and copper-clad laminates in energy storage batteries.

The invention provides a method for detecting organic iodine in seaweed, which belongs to the technical field of organic iodine detection, and comprises the following steps: drying, crushing and sieving a seaweed sample to obtain seaweed powder; performing hydrolysis and concentration on the seaweed powder, and fixing the volume to obtain a test solution; preparing various organic iodine standard substance solutions, sucking the standard substance solutions, injecting the standard substance solutions into a liquid chromatograph, and recording the relative retention time and the peak area of each organic iodine in the standard substance solutions; the high performance liquid chromatography conditions are as follows: a C18 chromatographic column is used, a 0.1% TFA aqueous solution is used as a mobile phase A, acetonitrile is used as a mobile phase B, and gradient elution is performed; the detection wavelength is 280 nm; and sucking the test solution, injecting the test solution into a liquid chromatograph, and carrying out qualitative and quantitative analysis on the organic iodine in the test solution. According to the high performance liquid chromatography analysis method established by the invention, the amino acid derivative organic iodine in the sample can be quantitatively determined without being interfered by L-tyrosine, L-tryptophan and L-phenylalanine in the sample.

The invention discloses a dual-mode detection kit for highly sensitive detection of ERalpha based on HRP-streptavidin. The dual-mode detection kit comprises the following raw materials: a glassy carbon electrode, 1-AP-CNHs, AuNPs, Sh-Apt, MCH, Bio-Ab, and HRP-streptavidin. The invention successfully constructs an electrochemical detection kit for detecting ERalpha with high sensitivity on the basis of the 1-AP-CNHs / AuNPs composite material. According to the invention, due to the redox activity of HRP-streptavidin, the HRP-streptavidin and H2O2 are subjected to redox reaction to generate a current signal; in addition, hydrogen peroxide can be catalyzed to generate. OH free radicals, and the free radicals can oxidize TMB to generate blue products, so that colorimetric reaction is initiated, and ERalpha can be detected more accurately. The electrochemical-colorimetric dual mode of the 3D 1-AP-CNHs / AuNPs composite material and the HRP-streptavidin for high-sensitivity detection of the ERalpha disclosed by the invention greatly improves the detection accuracy. The kit has the advantages of good selectivity, strong stability, high sensitivity, anti-interference performance, environmental protection and the like.

The invention discloses a HPLC determination method of tyloxapol content, comprising: preparing a tyloxapol inerting column solution, a tyloxapol reference solution, and a sample solution to be tested, respectively, wherein the concentration of the tyloxapol inerting column solution is greater than the concentration of the tyloxapol reference solution; firstly injecting the tyloxapol inerting column solution into a high performance liquid chromatograph for specific adsorption on the chromatographic column; then respectively performing high performance liquid analysis on the tyloxapol reference solution and the sample solution to be tested, and using an external standard method to determine the content of tyloxapol in the sample to be tested. The HPLC determination method of tyloxapol content of the invention is simple, fast, accurate, and highly sensitive, greatly shortening the time for testing, and is particularly suitable for determining the content of tyloxapol in a super-strong moisturizing lubricating eye drop, helping to improve the quality control and medication safety of tyloxapol liquid preparations, and can be used as an effective method for evaluating the quality of tyloxapol-containing preparations.

The invention relates to the technical field of medicine detection, and particularly discloses a method for detecting an antioxidant in butanedisulfonic acid ademetionine for injection. The method comprises the following steps: firstly, dissolving an ademetionine butanedisulfonate sample for injection in a solvent for injection for pretreatment, passing through a C18 solid-phase extraction column, and eluting with methanol, so that the antioxidant in the ademetionine butanedisulfonate sample for injection can be fully extracted, and the recovery rate is relatively high; then a C18 chromatographic column with a specific specification is adopted to carry out liquid chromatographic analysis on the test solution, and accurate detection of the antioxidant and the content of the antioxidant in the methionine butanedisulfonate for injection is realized at the same time; by limiting the conditions of the ultrahigh liquid chromatography, the peak response intensity of the test solution and the reference solution is improved, so that the detection precision is improved. The detection method can provide data support for the effective control of the quality of the ademetionine butanedisulfonate for injection, and ensures the safety of clinical medication.

The invention discloses a preparation method of an ITO / Au (at) CdS (at) ALP photoelectrochemical sensor for rapidly detecting organophosphorus pesticides. The method comprises the following steps: preparing Au (at) CdS NPs, and modifying the surface of the Au (at) CdS NPs with alkaline phosphatase to construct the sensor; different from a traditional photoelectrochemical sensor, a target substance is detected by causing change of system impedance or introducing (removing) a photoelectrochemical material, the intensity of a photoelectric signal is changed by changing the content of an electron donor in a system, and the preparation method is simple to operate, does not need a complex modification process, and is suitable for large-scale production. The method has good reproducibility and stability, and can rapidly, sensitively and accurately detect the organophosphorus pesticide.

The invention belongs to the field of analysis and detection, and particularly relates to a biosensor detection platform based on droplet evaporation and application of the biosensor detection platform in liver cancer marker AFP detection. The biosensor detection platform at least comprises a substrate, aptamer DNA, cholinesterase, a choline surfactant and streptavidin-coated magnetic beads, and through specific binding between the aptamer DNA and an object to be detected and hydrolysis reaction between the cholinesterase and the choline surfactant, the surface tension of liquid drops is changed by utilizing the surfactant, so that the detection sensitivity of the biosensor is improved. The dry area of the liquid drops on the surface of the substrate is changed, so that the to-be-detected object is rapidly detected. A biological sensing platform which is good in sensitivity and selectivity, rapid, simple and low in cost is created, and the method is particularly suitable for rapid on-site detection of AFP and can be applied to early screening and diagnosis of HCC.

This invention discloses hollow silver-platinum nanoalloy particles, their preparation method, and applications. The silver-platinum nanoalloy particles have a particle size of 30-60 nm, an overall hollow structure, and a porous surface distribution of silver and platinum fused together. The preparation method includes: using epigallocatechin gallate as the sole reducing agent and stabilizer, reducing silver nitrate in ethylene glycol to prepare monodisperse silver nanoparticles; then, at 60-80°C, slowly adding chloroplatinic acid solution in batches to the silver nanoparticle solution to carry out an in-situ exchange reaction; after the reaction, washing and separation yields the final product. This invention provides a green, simple, mild, and highly controllable preparation method. The prepared silver-platinum nanoalloy particles simultaneously possess excellent nanozyme catalytic activity and high-sensitivity surface-enhanced Raman scattering performance, achieving integrated synergistic catalytic and detection functions. It has broad application prospects in food safety testing, environmental monitoring, and biosensing.

The invention discloses a method for detecting related substances in domperidone tablets, and relates to the field of analytical chemistry. The detection method of the invention is as follows: the domperidone tablets are mixed with a diluent to obtain a test solution; the test solution is injected into a liquid chromatograph, loaded into a chromatographic column, and gradient eluted with mobile phase A and mobile phase B to separate domperidone from impurities in the test solution; mobile phase A and mobile phase B are respectively a mixture of 0.5% acetate buffer, methanol and acetonitrile, and the volume ratio of 0.5% acetate buffer, methanol and acetonitrile in mobile phase A is (59-61):35:(4-6); the volume ratio of 0.5% acetate buffer, methanol and acetonitrile in mobile phase B is (20-30):30:(40-50); a detector is used to test the separated domperidone and impurities to obtain a spectrum. The detection method of the invention realizes the effective separation of domperidone and various impurities, so that the separation between each spectrum peak is high, and the content of each impurity can be accurately detected.

This invention discloses a method for determining the content of Ophiopogonin C and Ophiopogonin D in Shengmai Yin soft capsules. The method involves simultaneous determination of the content of Ophiopogonin C and Ophiopogonin D in Shengmai Yin soft capsules using UPLC-MS / MS. An Agilent C18 column is used, with the mobile phase being a mixture of an aqueous solution containing 0.05% formic acid and 2 mmol / L ammonium acetate: acetonitrile-methanol (40:60, V / V) (30:70), at a flow rate of 0.3 mL / min. ESI-mode is used, with MRM monitoring of ion pairs. The test sample is prepared by reflux of 80% methanol for 15 min. This method is highly specific, sensitive, rapid, and accurate, effectively controlling the quality of Shengmai Yin soft capsules and filling the gap in the original standard for the quantitative determination of Ophiopogonin C and Ophiopogonin D.

The invention discloses a method for determining eight active components in dendrobium or cosmetics, which comprises the following steps: (1) pretreating a sample to obtain a solution for instrumental analysis; (2) detecting the eight active components in the sample by adopting a liquid chromatography-mass spectrometry method; the eight active ingredients are quercetin, naringenin, rutin, apigenin, caffeic acid, ferulic acid, syringic acid and chlorogenic acid. According to the method disclosed by the invention, extraction, chromatographic separation and mass spectrum quantification and qualitation of eight active substances including quercetin, naringenin, rutin, apigenin, caffeic acid, ferulic acid, syringic acid and chlorogenic acid in the dendrobe and the cosmetics are realized, and a technical support and a scientific basis are provided for research on efficacy relevance of active components of the dendrobe in the cosmetics.

The invention relates to a method for determining the content of phenolic acid components in salvia miltiorrhiza or salvia miltiorrhiza products by using high performance liquid chromatography. The method comprises the following steps: heating a sample in a 75% ethanol solution in a water bath, extracting to obtain nine phenolic acid components: tanshinol, caffeic acid, salvianolic acid B, rosmarinic acid, salvianolic acid A, 4-hydroxyphenyllactic acid, 4-coumaroyl-4 '-hydroxyphenyllactic acid, 4-coumaroyl-3', 4 '-dihydroxyphenyllactic acid and 3, 4-coumaroyl-4'-hydroxyphenyllactic acid, redissolving the sample to be detected with methanol, and detecting the content of the salvianolic acid in the sample to be detected. High performance liquid chromatography column separation detection is adopted, qualitative treatment is carried out by retention time, and quantification is carried out by an external standard-calibration curve method. The high performance liquid chromatography established by the invention can qualitatively and quantitatively detect the content of natural phenolic acid compounds in roots and leaves of salvia miltiorrhiza, and provides a standardized method for detecting phenolic acid characteristic active components in medicinal plant salvia miltiorrhiza and products thereof in the future; effective component detection guarantee is provided for the development of new salvia miltiorrhiza products, the analysis of a synthetic route of salvianolic acid compounds and the further improvement of the quality of salvia miltiorrhiza.

The application relates to the field of polyamide material analysis, and discloses application of an indantrione color developing reagent in determination of polyamide hydrolysis products and a method for determining the content of the polyamide hydrolysis products, wherein the indantrione color developing reagent contains an indantrione-ethylene glycol solution and an acetic acid-sodium acetate buffer solution. The application provided in the application adopts ethylene glycol as a single solvent of the indantrione, effectively inhibits oxidative degradation of the indantrione under high-temperature color developing conditions, and enables uniform color development of oligomers. The application does not add antioxidants, avoids interference with color developing reactions, is accurate and reliable in determination, is simple in preparation due to the single solvent, has a long storage time under light shielding at 4 DEG C, and can not only accurately determine monomers of polyamides and dimers to heptamers of the monomers, but also accurately determine various polyamide hydrolysis products, and has a good linear relationship.

The invention discloses a concrete saturation degree sensor based on the relative water contact area of completely polarized electrodes and a monitoring method.The concrete saturation degree sensor comprises a completely polarized electrode A and a completely polarized electrode B which are the same, and the completely polarized electrode A and the completely polarized electrode B are arranged in an interdigital mode; therefore, the water contact areas of the two electrodes are kept consistent. According to the method, the saturation degree of the hardened cement paste is calculated through the relative water contact area of the saturation degree sensor, the linear relation is good, the relinearity is good, response curves in the water absorption and dehydration process do not show obvious separation, the hysteresis quality is slight, and the salinity interference is slight. In general, the error of calculating the saturation degree from the relative water contact area by fitting the linear equation is within + / -10%, and the saturation degree sensor and the measurement method are low in cost and short in time consumption.

The invention belongs to the field of medicines, and particularly provides a method for determining the content of formaldehyde and acetaldehyde in polyethylene glycol and a preparation thereof by using a high performance liquid chromatography, and the specific chromatographic conditions are as follows: water and acetonitrile are used as mobile phases, and the flow velocity is 0.8-1.2 ml / min; and the detector is an ultraviolet detector. The method comprises the following steps: deriving trace formaldehyde and acetaldehyde in polyethylene glycol into substances with ultraviolet absorption by adopting a pre-column derivation method, then detecting through an ultraviolet detector, and calculating the content of formaldehyde and acetaldehyde in polyethylene glycol and a preparation thereof through an external standard method. The determination method has the advantages of high specificity, simplicity, convenience and rapidness in operation, high sensitivity and the like.

The invention belongs to the field of electrochemical analysis methods, and particularly relates to a Py-PB-COF / glassy carbon microsphere-ionic liquid paste electrode, a preparation method of the Py-PB-COF / glassy carbon microsphere-ionic liquid paste electrode and a detection method of uric acid. When being applied to detection of uric acid, the kit has good reproducibility, anti-interference performance and good sensitivity. The relative standard deviation RSD of six times of continuous measurement on the uric acid by using the same electrode is 1.54%; the relative standard deviation RSD value of the six electrodes prepared by the same method for parallel detection of uric acid is 0.92%, which indicates that the electrodes have good reproducibility in the aspect of uric acid detection; the detection limit is 0.5 mu M, which shows that the sensitivity is better. According to the preparation method, the Py-PB-COF / glassy carbon microsphere-ionic liquid paste electrode can be prepared only by mixing and grinding the nano graphite powder, the glassy carbon microspheres, the covalent organic framework material, the ionic liquid and the paraffin oil and then self-assembling, and the method is simple and easy to operate.

This invention provides a method for detecting tetrachloronaphthalene, a persistent organic pollutant in eel products. The specific steps include: eel sample preparation, target analyte extraction and matrix purification, followed by analysis using ultra-high performance liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry (UPLC-APCI-MS / MS). The measured peak area, fractionation ratio, and other relevant parameters are substituted into a specific formula to calculate the measured value of tetrachloronaphthalene in the test solution. This method fills the gap in my country's technology for detecting tetrachloronaphthalene-like persistent organic pollutants in eel products. It effectively eliminates interference from the complex matrix of eel products, accurately determines four tetrachloronaphthalene isomers in trace amounts, and possesses significant advantages such as high sensitivity, strong anti-interference ability, simple operation, and high detection accuracy.

This invention discloses a peroxynitroso anion fluorescent probe with AIE effect, its synthesis method, and its applications. The near-infrared fluorescent probe TPS was designed and synthesized in this invention. It is nearly colorless under ultraviolet light irradiation but emits bright yellow fluorescence upon reaction with peroxynitroso anions. Detection showed that TPS reacts with ONOO in a HEPES:acetonitrile = 1:1 system. ‑ The response time was 100 min, after adding 120 uL of 0.5×10 ‑3 mol·L ‑1 ONOO ‑ The reaction is complete. TPS exhibits high specificity, reacting only with peroxynitrosoanion among various anions, demonstrating high sensitivity. TPS reacts with ONOO at pH 4-8. ‑ The ion response exhibits good stability, and TPS is effective against ONOO. ‑ The response exhibits good linearity, with a detection limit of 0.08232. µmol·L ‑1 Probe TPS and ONOO ‑ The reaction, after different concentrations of THF, exhibited a typical AIE effect.

The invention provides a method for determining m-methylphenol residues in industrial wastewater, which comprises the following steps: S1, preparing a test solution: precisely weighing about 500mg of a test sample, placing the test sample in a 50mL measuring flask, dissolving with a dissolving solvent, diluting to a scale, and uniformly shaking; s2, the test solution is detected through liquid chromatography, the temperature of a chromatographic column of a liquid chromatograph is 20-30 DEG C, and the elution time is 25 minutes. S3, the test solution is detected through liquid chromatography, a mobile phase adopted in the liquid chromatography is composed of a phosphate buffer solution and acetonitrile according to the ratio of 807-827: 310-330, and the pH of the mobile phase is adjusted to 3.8-4.2 through phosphoric acid; according to the method, the theoretical concentration and the actually measured concentration have a good linear relation within the investigated concentration range, the average recovery rate is 99%, the detection limit is 2 ppm, and ICHQ3C data are infinitely required.