349results about How to "Good linear relationship" patented technology

The invention discloses a non-derivatization quantitative detection method for amino acid in a high-concentration protein preparation, which comprises the following steps: separating by using a graphitized carbon chromatographic column, adding an ion pair reagent trifluoroacetic acid into a mobile phase, and detecting by using an electric fog detector (CAD); the method comprises the following steps: preparing a plurality of groups of amino acid standard substance solutions with different concentration groups, wherein the concentration range is 1mmol / L-20mmol / L; drawing a quadratic equation standard curve of the amino acid standard product concentration and the peak area as a detection equation for detecting the amino acid concentration; the method comprises the following steps: diluting a test sample by using ultrapure water for multiple times, carrying out ultrafiltration centrifugal treatment, removing protein, introducing the sample, obtaining a corresponding peak area according to the spectrum integral of the test sample, and substituting the peak area into the detection equation to obtain the concentration of amino acid in the test sample. The method has the advantages of high detection precision, high detection efficiency and simple operation, and greatly reduces the adverse effect of high-concentration protein on the chromatographic column.

The invention discloses a method for simultaneously detecting residual quantity of 19 plant growth regulators in traditional Chinese medicinal materials, and belongs to the technical field of traditional Chinese medicinal material detection. The 19 plant growth regulators in the detected traditional Chinese medicinal materials are gibberellins, 4-chlorophenoxyacetic acid, 6-benzyladenine, paclobutrazol, 3-indolebutyric acid, thidiazuron, 2, 4-dihydroxyacetophenone and 2, 4-dihydroxyacetophenone respectively. The pesticide is prepared from the following raw materials: sodium 4-dichlorphenoxyacetate, forchlorfenuron, 3-indoleacetic acid, 4-fluorophenoxyacetic acid, isopentenyl adenine, imazalil, N-N '-diphenylurea, mepiquat chloride, diethyl aminoethyl hexanoate, 1-naphthylacetic acid amine, brassin, abscisic acid and 3-indolepropionic acid. According to the method, the QUECHERS traditional Chinese medicinal material sample on-machine pretreatment technology is applied, the ultra-high performance liquid chromatography-tandem mass spectrometry is established to measure the residual quantity of the 19 plant growth regulators in the medicinal materials, and the detection method is simple, clear, few in operation steps, extremely easy to operate, good in linear relation, good in sensitivity and high in accuracy.

The invention discloses an electrochemical detection method of dopamine, and belongs to the technical field of analysis. According to the method, SnS2 quantum dots are loaded on Zn-MOF and modified on an electrode, then gold nanoparticles and a sulfydryl-containing dopamine aptamer single chain are sequentially fixed on the electrode, an Apt / AuNPs / SnS2QDs (at) Zn-MOF / GCE composite electrode (in an open state) is formed, an electrochemical signal of the composite electrode is greatly improved, and based on specific recognition between dopamine and an aptamer chain, the electrochemical performance of the composite electrode is improved. A large cavity of Zn-MOF is used for packaging SnS2 quantum dots with excellent electrochemical properties, electrochemical signal amplification is achieved, when a target object dopamine exists, dopamine can quench an ECL signal, an electric signal is obviously reduced (in a closed state), and therefore a linear relation between the change of the electrochemical signal and the concentration of dopamine is constructed, and the electrochemical property of the electrochemical signal is improved. Therefore, high-sensitivity and high-selectivity detection of dopamine is realized.

The invention discloses a method for measuring the content of rare earth elements in a single rare earth compound by turbidimetry. Comprising the following steps: 1, preparing a fluoride solution and a 50% nitric acid aqueous solution; 2, preparing a rare earth element standard solution; step 3, testing the rare earth compound sample by using turbidimetry; and 4, calculating a detection result: calculating the content of the rare earth elements in the sample according to a formula. The method can be used for testing the content of single rare earth with different content levels in the sample. The method has the advantages of wide linear range, high precision, good accuracy, low cost, simplicity in operation, high detection efficiency and the like.

The invention relates to the technical field of a preparation method of a determination kit for in-vitro determination of adiponectin in a human body, and particularly discloses a preparation method of a determination kit for in-vitro determination of adiponectin in a human body, which comprises the following steps: a coupling process; an S-shaped bent pipe is installed on the side connector, an upper connector is kept in an open state, a perfusion device adds 0.3-0.4 volume of a sealing buffer solution into the glass bottle, and 0.1-0.2 volume of the sealing buffer solution is injected into the glass bottle through an injection connector; and a storage process: closing the filling interface, opening the cover body, installing the up-down extending pipe, covering the cover body, opening the filling interface, carrying out resuspension ultrasonic treatment by using the storage buffer solution, and carrying out centrifugal washing for three times, thereby finally obtaining the adiponectin latex antibody. The device is easy and convenient to operate, simple in structure, independent of the manual control level and high in preparation efficiency, the situation that materials are left in a small-size glass bottle, consequently, the material ratio is difficult to control, and a small amount of confining liquid is needed is avoided, and the prepared adiponectin latex antibody is good in accuracy.

The invention relates to a flexible pressure sensor and a preparation method thereof. The preparation method of the flexible pressure sensor comprises the steps of providing a flexible conductive substrate, etching one surface of the flexible conductive substrate by adopting first laser to form a first microstructure with the same height, and then etching the side wall of the first microstructure by adopting second laser to form a hierarchical microstructure with at least one annular groove, a first flexible substrate is obtained; providing a second flexible substrate, wherein the second flexible substrate comprises a second flexible substrate and an electrode arranged on one surface of the second flexible substrate; and arranging a second flexible substrate on the first flexible substrate in a laminated manner, and enabling an electrode to be in contact with the hierarchical microstructure, thereby obtaining the flexible pressure sensor. The flexible pressure sensor prepared by the preparation method not only can maintain high sensitivity, but also has a wide detection range, and can maintain a good linear relation of output signals.

The invention relates to a preparation method and application of a novel lactic acid detection fluorescent probe, and belongs to the technical field of fluorescent probes. The preparation method of the fluorescent probe comprises the following steps: synthesizing a core-shell type up-conversion nano material CS-UCNPs; carrying out surface modification on the core-shell type up-conversion nano material CS-UCNPs; coupling of the core-shell type up-conversion nano material CS-UCNPs and an L-lactic acid aptamer is carried out; and synthesizing the magnetic molybdenum disulfide nanosheet MoS2. The L-lactic acid aptamer, the core-shell UCNPs and the magnetic MoS2 are combined for high-sensitivity detection of the lactic acid content, and compared with a traditional blood lactic acid detection method, the method has the advantages of being non-invasive, non-invasive and the like; and the technology has the advantages of simplicity in operation, high specificity, quick response and the like, has a wider detection range and a lower detection limit, and is expected to be applied to actual sample detection work.

The invention relates to the field of photoelectric sensors, in particular to a photoelectric sensor based on a bismuth sulfide composite material and application of the photoelectric sensor in norfloxacin detection, and a preparation method of the photoelectric sensor comprises the following steps: preparing the bismuth composite material; modifying the bismuth composite material with gold nanoparticles; modifying a fluorine-doped tin oxide glass electrode; the photoelectric sensor provided by the invention is combined with the gold nanoparticle sol, and an electric field formed by surface plasmon resonance accelerates the migration of electrons, enhances the optical absorption of the whole material and improves the photoelectric response. The photoelectric signal intensity has a good linear relationship with the concentration of norfloxacin in a certain range, so that the method has good reproducibility and relatively high detection sensitivity, can realize effective detection of norfloxacin, and has good application value and application prospect in the fields of environment, biology, medicine and the like.

The invention discloses a method for determining theabrownin content in Liubao tea, and belongs to the technical field of tea component determination. The method can accurately determine theabrownin content in Liubao tea, and the method has good repeatability, recovery rate, linear relationship and sensitivity, and has high specificity for determining theabrownin in Liubao tea. The method comprises the following preparation steps: (1) taking a plurality of first-grade Liubao teas and mixing them to prepare a theabrownin reference substance, and calibrating the purity of theabrownin, and using the calibrated theabrownin standard substance to prepare a theabrownin concentration-absorbance curve of a standard Liubao tea sample; (2) taking a Liubao tea sample to be determined, soaking and extracting it, adding ethanol, mixing and standing it, and obtaining a precipitate of the sample to be determined after centrifugation; (3) dissolving and diluting the precipitate of the sample to be determined, and putting it into an ultraviolet spectrophotometer to determine the absorbance of the sample to be determined; (4) using the theabrownin concentration-absorbance curve of the standard Liubao tea sample to calculate the theabrownin concentration corresponding to the absorbance of the sample to be determined, and then calculating the theabrownin content.

The invention relates to a fluorine-based three-dimensional covalent organic framework material as well as a preparation method and application thereof, and belongs to the technical field of covalent organic framework materials. Under the action of a catalyst, a first monomer (the first monomer is selected from a regular tetrahedron-containing amino monomer or a regular tetrahedron-containing aldehyde group monomer) and a second monomer (the second monomer is selected from a fluorine-containing aldehyde group monomer or a fluorine-containing amino monomer) are subjected to a condensation reaction in an organic solvent to obtain the fluorine-based three-dimensional covalent organic framework material. The method comprises the following steps of: preparing a solid-phase microextraction probe by taking the perfluorinated compound as an adsorbent through in-situ growth on the surface of a probe matrix, and then combining the solid-phase microextraction probe with an open-type mass spectrum to detect trace perfluorinated compounds in a complex matrix. The fluorine-based three-dimensional covalent organic framework material disclosed by the invention is good in chemical stability and high in hydrophobicity, has a good adsorption effect on perfluorinated compounds, and is high in repeated utilization rate.

The invention relates to the technical field of zearalenone detection, in particular to zearalenone immunomagnetic beads as well as a preparation method and application thereof. The method specifically comprises the following steps: (1) cleaning N-hydroxysuccinimide activated agarose magnetic beads with ethanol, removing ethanol, adding a 2-morpholinoethanesulfonic acid buffer solution and a ZEN monoclonal antibody for mixed incubation, and discarding an upper solution; and (2) adding a glycine-containing buffer solution into the incubated magnetic beads, mixing, sealing, carrying out magnetic separation, and leaching to obtain the ZEN immunomagnetic beads. According to the method, IMBs high-flux full-automatic purification is combined with a high performance liquid chromatograph, the method which is rapid and convenient and can automatically treat ZEN in feed and feed raw material samples in batches is established, and the first feed industry association group standard about the IMBs purification technology is researched and formulated on the basis of the method; and a method reference is provided for prevention and control of fungaltoxin pollution in basic detection laboratories and feed enterprises.

The invention discloses a preparation method of a Mn-AMP metal organic gel nano-enzyme and application of the Mn-AMP metal organic gel nano-enzyme in colorimetric sensing, and the Mn-AMP metal organic gel with peroxidase-like activity is prepared by taking adenosine-5 'monophosphate (AMP) as a ligand. A colorimetric biosensor used for detecting ascorbic acid (AA) is constructed on the basis, a portable detection method is designed in combination with a smart phone application program 'Color Grab', and a new solution is provided for on-site rapid detection of AA. The preparation method disclosed by the invention is mild in condition and simple in synthesis path, has good water solubility and biocompatibility, is an ideal choice for nano-enzyme materials, and shows a good application prospect in the aspects of biosensing, bacteriostasis and the like.

The invention discloses a hydroxyproline rapid detection molecularly imprinted electrochemical sensor as well as preparation and application thereof, and relates to the field of quality safety monitoring of medicinal plants. The preparation method of the molecularly imprinted electrochemical sensor comprises the following steps: preparing the Fc-MWCNTs composite material, preparing the Fc-MWCNTs / GCE, and preparing the molecularly imprinted electrochemical sensor MIP-Fc-MWCNTs (at) GCE. According to the invention, MWCNTs with good conductivity and Fc which has good stability and oxidation-reduction property and is used as an excellent electron transfer medium are used as electrode substrate materials; and the Fc-MWCNTs and the MWCNTs are covalently bonded through an amide reaction to prepare the composite material Fc-MWCNTs which has excellent electron transfer medium characteristics, low potential reproducibility and strong stability and can remarkably improve the electron transfer rate between the electrode surface and an electrolyte. The modified composite material Fc-MWCNTs is modified on the surface of GCE, so that the molecularly imprinted electrochemical sensor MIP-Fc-MWCNTs (at) GCE with high sensitivity, high selective recognition and fast detection is constructed, and the molecularly imprinted electrochemical sensor is used for detecting an index component Hyp of cornu cervi pantotrichum.

The invention relates to a method for simultaneously detecting 17 organophosphate substances in urine, and belongs to the technical field of detection. The invention relates to a method for simultaneously detecting 17 organophosphorus ester substances in urine, and the 17 organophosphorus ester substances are diethyl phosphate, di (2-chloroethyl) phosphate, bis (1-chloro-2-propyl) phosphate, diphenyl phosphate, dibutyl phosphate, xylene phosphate, di-p-tolyl phosphate, triphenylphosphine oxide, tri-(2-chloroisopropyl) phosphate, 2, 3, 4-trimethyl-1, 3-pentanediol monoisobutyrate, 2, 3, 4-trimethyl-1, 3-pentanediol monoisobutyrate, 2, 3, 4-trimethyl-1, 3-pentanediol monoisobutyrate, 2, 3, 4-trimethyl-1, 3-pentanediol monoisobutyrate and 2, 3, 4-trimethyl-1, 3-pentanediol monoisobutyrate. The invention relates to a flame-retardant and flame-retardant composite material which is prepared from the following components: 1, 2-dichloromethyl-trimethylene-bis [bis (2-chloroethyl) phosphate], bis (2-ethylhexyl) phosphate, tris (1, 3-dichloro-2-propyl) phosphate, triisobutyl phosphate, tributyl phosphate, tris (2-n-butoxyethyl) phosphate, resorcinol tetraphenyl diphosphate and bisphenol A bis diphenyl phosphate. The method has the characteristics of simplicity, convenience, high efficiency, accuracy, sensitivity, economy and the like.

The invention provides a crystal phase impurity analysis method, and belongs to the technical field of analytical chemistry. The method comprises the following steps: acquiring an X-ray diffraction pattern of a sodium zirconium cyclosilicate sample to be detected; according to the standard curve or standard equation of the crystal phase impurities and the X-ray diffraction pattern of the sodium zirconium cyclosilicate sample to be detected, the content of the crystal phase impurities in the sodium zirconium cyclosilicate sample to be detected is obtained; the standard curve is obtained by fitting the ratio of the mass of the crystal phase impurities to the mass of the sodium zirconium cyclosilicate in a standard curve sample and the ratio of the diffraction intensity of characteristic diffraction peaks of the crystal phase impurities to the diffraction intensity of characteristic diffraction peaks of the sodium zirconium cyclosilicate in an X-ray diffraction pattern of the standard curve sample; the standard equation is a linear equation corresponding to the standard curve; the standard curve sample is a mixture of a blank sodium zirconium silicate sample and a crystal phase impurity reference substance. By adopting the method disclosed by the invention, the content of the crystal phase impurities such as CPA and CPB in the sodium zirconium cyclosilicate can be simply, conveniently, quickly and accurately detected.

The invention belongs to the technical field of medicine analysis, and particularly relates to a method for detecting an impurity A, an impurity B, an impurity C and an impurity D. High performance liquid chromatography is adopted for detection, and chromatographic conditions are as follows: a Titank Phenyl-Hexyl chromatographic column is adopted as a chromatographic column; acetonitrile and a buffer solution with the ratio of 4: 96 v / v are used as a mobile phase for isocratic elution, and every 1 L of the buffer solution contains 8.00 g of sodium 1-octanesulfonate, 37.00 mg of EDTA-disodium, 10 mL of perchloric acid and 2 mL of phosphoric acid; the flow velocity of the mobile phase is 0.6-1.0 mL / min, the column temperature is 15-25 DEG C, the sample size is 5-15 mL, an ultraviolet detector is used for detection, and the detection wavelength is 210-220 nm; the method can detect impurities A, B, C and D in the zoledronic acid bulk drug, and can be used for quality control of the zoledronic acid bulk drug.

The invention discloses a method for determining the contents of ester solvents and additives in a sodium-ion battery electrolyte by GC-MS. Through preparation of a diluent, selection of a program method and control of conditions, the types and contents of the solvents and the additives in the sodium-ion battery electrolyte can be rapidly and accurately determined in one step. Therefore, the method can be widely applied to the field of component analysis of the sodium-ion battery electrolyte.

The invention relates to the technical field of cell apoptosis detection, in particular to a kit for cell apoptosis detection and a product thereof. The Annexin V-FITC provided by the invention can well realize the detection of apoptosis, has obvious linear correlation with the apoptosis rate, and can realize the high-sensitivity detection of the occurrence of apoptosis. The method and the kit for detecting cell apoptosis can effectively identify phosphatidylserine exposed on the outer surface of apoptotic cells, and have more detection advantages compared with Annexin V full length. The sensitivity and the cell apoptosis rate of the detection method have a good linear relationship, and the detection method has a relatively great application prospect.

The application belongs to the technical field of fluorescent probe detection, and discloses a bimetallic nanocluster fluorescent probe compound and application thereof in preparation of a product for rapidly detecting acetylene. The fluorescent probe compound is a gold-copper bimetallic nanocluster solution modified by adamantane thiol, which is used as a fluorescent probe for acetylene detection. The solution has a clear and controllable preparation process. Through specific proportioning and reaction conditions of cuprous chloride, adamantane thiol, tetrachloroauric acid, sodium borohydride and other reagents, a probe solution with stable fluorescence characteristics can be prepared. The bimetallic nanocluster fluorescent probe compound is applied in preparation of a product for rapidly detecting acetylene. Through specific combination of fluorescent molecules and acetylene gas to trigger the secondary effect of fluorescence quenching, detection is realized, and other substances cannot trigger quenching. At a wavelength of 700 nm, the linear relationship (R 2 >0.99) between the fluorescence intensity and the acetylene concentration realizes accurate quantification of the acetylene concentration, and the minimum detection limit is 0.026 muL / L.

The present invention discloses a method for analyzing the chemical composition and content determination of Chlorophytum comosum based on liquid chromatography-quadrupole-electrostatic field orbital trap tandem mass spectrometry, including preparing a reference solution, preparing a test solution, and chromatographic analysis, wherein the chemical composition analysis also includes mass spectrometry analysis and data processing. This application is the first to simultaneously quantitatively analyze three types of components in Chlorophytum comosum, namely flavonoids, phenylethanoid glycosides, and organic acids. The results show that the contents of these five components in medicinal materials from different origins vary greatly, which may be due to factors such as different origins, growth environments, climatic conditions, and harvesting times that affect the accumulation of compounds in plants. The specific reasons need further study.

This invention relates to the field of electroplating solution analysis technology, and in particular to a rapid analytical method for polyethylene glycol (PEG) additives in copper electrodeposition solutions. The method involves protonated PEG forming a macromolecular complex colloid with phosphomolybdate under acidic conditions of pH 1.0–2.0. This colloid exhibits maximum absorption at 372 nm, and the absorbance value is directly proportional to the PEG concentration in the formed complex colloid. This allows for quantitative analysis of PEG in the copper electrodeposition solution. Because the phosphomolybdate-PEG complex colloid selected in this invention has excellent selectivity, and no sample pretreatment is required, this invention offers advantages such as ease of operation, speed, high sensitivity, and good selectivity. It is particularly suitable for intermediate control analysis in production environments, such as for electroplated copper interconnects in chips and printed circuit boards, as well as electrolytic copper foil for negative electrode current collectors and copper-clad laminates in energy storage batteries.

The invention provides a method for detecting organic iodine in seaweed, which belongs to the technical field of organic iodine detection, and comprises the following steps: drying, crushing and sieving a seaweed sample to obtain seaweed powder; performing hydrolysis and concentration on the seaweed powder, and fixing the volume to obtain a test solution; preparing various organic iodine standard substance solutions, sucking the standard substance solutions, injecting the standard substance solutions into a liquid chromatograph, and recording the relative retention time and the peak area of each organic iodine in the standard substance solutions; the high performance liquid chromatography conditions are as follows: a C18 chromatographic column is used, a 0.1% TFA aqueous solution is used as a mobile phase A, acetonitrile is used as a mobile phase B, and gradient elution is performed; the detection wavelength is 280 nm; and sucking the test solution, injecting the test solution into a liquid chromatograph, and carrying out qualitative and quantitative analysis on the organic iodine in the test solution. According to the high performance liquid chromatography analysis method established by the invention, the amino acid derivative organic iodine in the sample can be quantitatively determined without being interfered by L-tyrosine, L-tryptophan and L-phenylalanine in the sample.

The invention discloses a dual-mode detection kit for highly sensitive detection of ERalpha based on HRP-streptavidin. The dual-mode detection kit comprises the following raw materials: a glassy carbon electrode, 1-AP-CNHs, AuNPs, Sh-Apt, MCH, Bio-Ab, and HRP-streptavidin. The invention successfully constructs an electrochemical detection kit for detecting ERalpha with high sensitivity on the basis of the 1-AP-CNHs / AuNPs composite material. According to the invention, due to the redox activity of HRP-streptavidin, the HRP-streptavidin and H2O2 are subjected to redox reaction to generate a current signal; in addition, hydrogen peroxide can be catalyzed to generate. OH free radicals, and the free radicals can oxidize TMB to generate blue products, so that colorimetric reaction is initiated, and ERalpha can be detected more accurately. The electrochemical-colorimetric dual mode of the 3D 1-AP-CNHs / AuNPs composite material and the HRP-streptavidin for high-sensitivity detection of the ERalpha disclosed by the invention greatly improves the detection accuracy. The kit has the advantages of good selectivity, strong stability, high sensitivity, anti-interference performance, environmental protection and the like.

The invention relates to the technical field of pharmaceutical analysis, in particular to a method for detecting related substances in etamsylate bulk pharmaceutical chemicals. The invention discloses a method for detecting related substances in etamsylate bulk pharmaceutical chemicals, which comprises the following steps: detecting related substances in etamsylate bulk pharmaceutical chemicals by high performance liquid chromatography, the related substances comprising impurities as shown in formula 1 and / or impurities as shown in formula 2; the invention provides a method for detecting related substances in etamsylate bulk drugs, the method is good in separation degree, simple and rapid, strong in specificity, good in accuracy, high in precision and good in linear relation, and can be used for necessary quality control of the bulk drugs, and the impurity determination method is high in detection sensitivity and stable in base line.

The invention discloses a method for simultaneously detecting a plurality of impurities in a rafenasin solution for inhalation, which can simultaneously detect the impurities A, B, C, D, E and F in the rafenasin solution for inhalation, and the method is rapid, simple and convenient, and has good accuracy, specificity and linearity.

The invention belongs to the technical field of fluorescent nano materials and medical examination, and particularly relates to a high-selectivity carbon dot for detecting flavonol compounds in colorimetric and fluorescent dual modes as well as a preparation method and application of the high-selectivity carbon dot. The preparation method of the carbon dots comprises the following steps: preparing a (PEI) solution; the preparation method comprises the following steps: dispersing D-threonine (D-Thr) and a polyethyleneimine (PEI) solution in deionized water, performing ultrasonic treatment, and performing heating reaction; after the reaction, cooling to room temperature, centrifuging, taking supernate, and filtering to obtain a B-CDs solution; the B-CDs solution is subjected to freeze drying, and the yellowish-brown powder B-CDs carbon dots are obtained. The high-selectivity carbon dot based on polyethyleneimine prepared by the invention shows good water solubility, excellent fluorescent property and optical stability, has been successfully applied to quantitative detection of three flavonol compounds including morin, quercetin and kaempferol in complex matrixes such as human serum and urine, and shows a good linear relationship in a range of 0.5-100 [mu] M.

The invention discloses a HPLC determination method of tyloxapol content, comprising: preparing a tyloxapol inerting column solution, a tyloxapol reference solution, and a sample solution to be tested, respectively, wherein the concentration of the tyloxapol inerting column solution is greater than the concentration of the tyloxapol reference solution; firstly injecting the tyloxapol inerting column solution into a high performance liquid chromatograph for specific adsorption on the chromatographic column; then respectively performing high performance liquid analysis on the tyloxapol reference solution and the sample solution to be tested, and using an external standard method to determine the content of tyloxapol in the sample to be tested. The HPLC determination method of tyloxapol content of the invention is simple, fast, accurate, and highly sensitive, greatly shortening the time for testing, and is particularly suitable for determining the content of tyloxapol in a super-strong moisturizing lubricating eye drop, helping to improve the quality control and medication safety of tyloxapol liquid preparations, and can be used as an effective method for evaluating the quality of tyloxapol-containing preparations.

The invention discloses the creation and application of an aromatic amide biosensor, and belongs to the field of bioengineering technology. The biosensor in the present invention is based on the amino acid motif of the transcriptional regulator AmiC-AmiR derived from Pseudomonas aeruginosa, and the RBS of AmiC and AmiR are optimized respectively, and the tyrosine at position 83 of AmiC is mutated to isoleucine, and the threonine at position 233 is mutated to cysteine. Compared with the wild-type transcriptional regulator AmiC-AmiR, the biosensor provided by the present invention has a good linear relationship in its responsiveness to aromatic amides, and the response range is between 0 and 10 mM after the optimization of RBS. The biosensor that can respond to aromatic amides provided by the present invention can be used for the detection of aromatic amides, screening strains that metabolize aromatic amides, or laying the foundation for subsequent research on aromatic amide production metabolic pathways, and has a wide range of application scenarios.

The invention relates to the technical field of medicine detection, and particularly discloses a method for detecting an antioxidant in butanedisulfonic acid ademetionine for injection. The method comprises the following steps: firstly, dissolving an ademetionine butanedisulfonate sample for injection in a solvent for injection for pretreatment, passing through a C18 solid-phase extraction column, and eluting with methanol, so that the antioxidant in the ademetionine butanedisulfonate sample for injection can be fully extracted, and the recovery rate is relatively high; then a C18 chromatographic column with a specific specification is adopted to carry out liquid chromatographic analysis on the test solution, and accurate detection of the antioxidant and the content of the antioxidant in the methionine butanedisulfonate for injection is realized at the same time; by limiting the conditions of the ultrahigh liquid chromatography, the peak response intensity of the test solution and the reference solution is improved, so that the detection precision is improved. The detection method can provide data support for the effective control of the quality of the ademetionine butanedisulfonate for injection, and ensures the safety of clinical medication.

The invention discloses a preparation method of an ITO / Au (at) CdS (at) ALP photoelectrochemical sensor for rapidly detecting organophosphorus pesticides. The method comprises the following steps: preparing Au (at) CdS NPs, and modifying the surface of the Au (at) CdS NPs with alkaline phosphatase to construct the sensor; different from a traditional photoelectrochemical sensor, a target substance is detected by causing change of system impedance or introducing (removing) a photoelectrochemical material, the intensity of a photoelectric signal is changed by changing the content of an electron donor in a system, and the preparation method is simple to operate, does not need a complex modification process, and is suitable for large-scale production. The method has good reproducibility and stability, and can rapidly, sensitively and accurately detect the organophosphorus pesticide.