79results about How to "Realize qualitative analysis" patented technology

The invention belongs to the fields of the state monitoring and the fault diagnosis of vehicles, ships, engineering machinery, locomotive cars, engines, and the like by applying noise signals, in particular to a mechanical fault judgment system and method based on noise test, particularly suitable for complicated mechanical machinery which can not be provided with various sensors. The system comprises a high-frequency microphone, a sound card, a PC (Personal Computer) and a telescopic bracket, wherein the high-frequency microphone is fixed on the telescopic bracket and connected with the sound card, the sampling frequency of the high-frequency microphone can meet the requirement of the Nyquist theorem, and the sound card is connected with the PC. On the basis of combing the extraction andfractal algorithm of an energy band, noise signals in machinery operation are acquired and calculated to test mechanical faults by using the method; the system is simple to set, the economic cost is decreased, and the operation safety and the fault diagnosis accuracy are improved.

The invention discloses a method for evaluating the innovation degree of text information. The method for evaluating the innovation degree of the text information comprises the steps of performing word segmenting pre-processing on acquired text data, and training an LDA model by using preprocessed data to realize an industry-themed classification for distinguishing texts in different industries so as to enable a trained model to contain model files of keywords of each industry category; establishing a knowledge network composed of key knowledge and technical information extracted from the preprocessed data according to a time sequence, and continuously updating the knowledge network at regular time intervals; utilizing the preprocessed data sorted according to the time sequence to extract attribute information of each piece of literature, establishing a literature text structure network, and determining an innovation type of each piece of literature; using the trained LDA model to analyze which industry technology keywords or knowledge keywords in patent data belong to respectively, and combining the attribute information of the literature text structure network so as to constitute multidimensional indexes for the innovation degree evaluation.

This invention relates to Kort inertia force experiment device, which comprises host rack, level rotation rack, plat board, trigger device, controller device, wherein, the rotation rack is fixed with drive plat on the vertical rotation device; the switch gear guide raid is fixed on the platform through single force sensor; the said rail is fixed with electrical trolley. The device can realize dynamic co ordinate's rotation and dynamic direction at any angle to Kort force demonstration.

The invention discloses a method for synchronously determining monosaccharide, uronic acid and saccharic acid in a wood fiber material reaction system. The method comprises the steps as follows: pretreating the wood fiber material reaction system to obtain a solution to be determined; determining standard samples of monosaccharide, uronic acid and saccharic acid by adopting an integrated pulse amperometric detection method and a chromatographic peak integration method and utilizing a high-performance liquid ion exchange chromatograph, thus obtaining a standard equation; determining the solution to be determined by utilizing the high-performance liquid ion exchange chromatograph and calculating the content of each component by utilizing the standard equation; and separating and quantifyingxylose and mannose in the solution to be determined by utilizing a high-performance liquid chromatography, and then amending the result of the high-performance liquid ion exchange chromatography. Themethod for synchronously, correctly and quantitatively determining the monosaccharide, the uronic acid and the saccharic acid is established for the first time, the separation degrees and the detection efficiencies of eight types of materials are improved greatly and the synchronous, correct and quantitative determination on nine types of materials in the wood fiber material reaction system can be realized under the combination of the high-performance liquid ion exchange chromatography; and the method has significance to matter changing, and component analysis and determination on products and intermediate products in bio-refinery process of the wood fiber material.

The invention discloses a method for analyzing short-chain chlorinated paraffin in an environment sample by a comprehensive two-dimensional gas chromatography-flight time mass spectrometry, which belongs to the technical field of analytical testing. The method comprises the following steps: 1) performing enrichment extraction on SCCPs in the environment sample; 2) employing a comprehensive two-dimensional gas chromatography-electron bombardment ionization source-low resolution time-of-flight mass spectrometer for detection; 3) employing library searching and a retention time index for determining the nature of the SCCPs component in the sample; and 4) employing an internal standard curve method for determining total amount of SCCPs. The pre-treatment method has the advantages of high extraction efficiency, good purifying effect, and wide application scope; the used apparatus has the advantages of high analysis efficiency, good separating effect, and strong anti-interference capability;the qualitative method is fast and accurate, and does not depend on a standard substance; the quantification method has the advantages of low detecting limit, low sensitivity, wide linearity range, and good repeatability, and the analysis requirement of SCCPs in the practical environment sample can be satisfied.

The invention discloses a laser Raman spectrum detection method for quickly analyzing content of basic flavine in soybean hull and relates to the technical field of food detection. The laser Raman spectrum detection method includes the following steps: (1), determining basic flavine characteristic Raman peak: 650cm-1+ / -3cm-1; (2), drawing a basic flavine standard curve; (3), preparing a to-be-detected sample solution; (4), taking the to-be-detected sample solution, adding nano enhanced reagent and inorganic salt flocculant, rocking uniformly, using a laser Raman spectrum instrument to detect, and acquiring a Raman spectrogram of a sample, wherein the nano enhanced reagent is one of gold, silver or copper nano sol; (5), performing qualitative determination; (6), performing quantitative determination. The laser Raman spectrum detection method has the advantages of being simple in operation and accurate, quick, lossless and low-cost in detection, is suitable for on-site emergency detection, enterprise self-control detection, large-scale sample screening detection and the like, can be used for both qualitative analysis and quantitative analysis, problematic food can be effectively recognized, and consumer rights and interests can be protected.

The invention discloses a LC-Q-TOF (liquid chromatography quadrupole time-of-flight) detection method for the fipronil in an egg and an egg product. The method comprises the following steps of: sequentially adding a 0.1% formic acid acetonitrile solution, the sodium chloride, and the anhydrous magnesium sulfate to the egg and the egg product to be detected; vortexing, mixing, and centrifuging to obtain the supernatant liquid; performing supercritical CO<2> extraction on the supernatant liquid; adding the 0.1% formic acid acetonitrile solution for constant volume after the extract liquid is dried at room temperature under nitrogen; and injecting the liquid to be detected after the constant volume into LC-Q-TOF for detection. According to the LC-Q-TOF detection method for the fipronil in theegg and the egg product, the mass spectrum of the liquid to be detected is obtained by LC-Q-TOF, and the qualitative analysis of components in the liquid to be detected is realized, thereby screeningwhether the egg and the egg product to be detected contain the fipronil. At the same time, according to a chromatogram, the concentration of the fipronil-containing in the egg and the egg product tobe detected is calculated. The detection limit of the detection method is 0.4ug / Kg, which is much better than other detection methods. The detection method not only can accurately screen the qualitative fipronil in the egg and the egg product to be detected, but also accurately quantify the fipronil, which is beneficial for the detection of the fipronil in the egg and the egg product.

The invention relates to a computer readable storage medium and a heart rate analyzer using the same and belongs to the heart rate sensing technological field. The heart rate data output process of the heart rate analyzer includes the following steps that: step 1, an RRI sequence is obtained according to data outputted by a PPG heart rate sensor; step 2, de-trending analysis is performed on the RRI sequence; step 3, re-sampling is carried out, so that a uniformly-distributed RRI sequence can be obtained; step 4, Hamming windowing is carried out to divide the uniformly-distributed RRI sequenceinto small overlapped segments; step 5, power spectrum estimation is performed on each sequence segment; step 6, the average values of the power spectrum estimates of each frequency band are calculated; and step 7, integral processing is performed on the average values of the power spectrum estimates of each frequency band, so that the power values of an ultra-low frequency band (ULF), a very-lowfrequency band (VLF), a low frequency band (LF), and a high frequency band (HF) are obtained, and the power values of the four frequency bands are added, so that signal total power TP can be obtained.With a simpler method adopted, the heart rate analyzer can process acquired heart rate data into heart rate data with higher accuracy which can be used by a user.

The invention relates to a quick detection technology of residual sulfur dioxide in extract of natural plant, and specially relates to a detection method adopting Raman spectrum characteristic peak signal intensity, and a residual sulfur dioxide detection method suitable for the extract of the natural plant in the healthcare food. By adopting the Raman spectrum characteristic peak signal intensity, the 618cm-1(+ / -3cm-1) is used as the characteristic Raman peak, the qualitative analysis is accurate, the whole detection process is accomplished in 15 min, and the quick qualitative and quantification, the convenience and the low cost are realized.

A reconfigurable assembly system modeling method based on Agent-based timed colored Petri net in the field of automatic control and artificial intelligence technology, aiming at the complexity, reconfiguration, intelligence and autonomy of reconfigurable assembly system Features: Based on the classification of assembly resources, the internal behavior of assembly resource Agent is encapsulated, the ATCPN model of assembly resource Agent is established, and the reconfigurable assembly unit ATCPN model is synthesized through transition transition. According to the logical relationship between each reconfigurable assembly unit, the message transmission relationship of the whole system is determined, and on this basis, the rapid reconfiguration of the assembly system is realized. Analyze the performance indicators of the ATCPN model of the reconfigurable assembly system, and propose the mapping rules from the AUML model to the ATCPN model and the interactive protocol modeling method based on ATCPN, and the deadlock existing in the model can be solved by introducing the corresponding multi-agent interactive protocol model and conflict.

The invention discloses a disease prediction method and system based on medical big data. The method comprises the following steps: importing mass spectrum data of mainstream mass spectrum instrumentmanufacturers, and completing the conversion from original mass spectrum data of the manufacturers to a general data format; realizing automatic labeling and qualitative analysis of endogenous compounds in the mass spectrum through a metabolite compound database according to the spectrum information of the endogenous metabolites of the individual human body and a compound search matching algorithm; wherein the loading is used for effectively managing huge and complex human body metabonomics samples and carrying out data mining and modeling, and the loaded data types mainly comprise targeted and non-targeted metabonomics mass spectrum original data and labeling results; performing standardized cleaning, compound alignment and qualitative and quantitative analysis on complex multi-sample data, and performing correction by adopting a multi-class algorithm; and adopting a machine learning model training set algorithm with different types of mass spectrum data processing characteristics tocomplete the whole process of training, publishing and maintaining the user disease prediction model.

The invention relates to a constructing method of a zebra fish bacterial infection model and application of the animal model to bacterial infection research and antibiotic screening. The constructing method of the zebra fish bacterial infection model mainly includes the steps of zebra fish selection, bacterium injection, image analysis and / or microwell plate analysis and statistical analysis. The constructing method has the advantages of simplicity, convenience, rapidity, economy, high efficiency, high throughput and the like, and the model can be used for bacterial infection research and antibiotic screening. The constructing method and application of the zebra fish bacterial infection model are of great significance to acceleration of research and development on antibiotics and improvement on treatment of patients suffering from bacterial infection.

The invention discloses a quick extraction and detection method of diethyl yellow in dried bean curd, and relates to the technical field of food detection. The method comprises the following steps: determining characteristic raman peak 990+ / -3 cm<-1> of the diethyl yellow; drawing a diethyl yellow standard curve; weighing the bean curd, mixing with diatomite and then adding in an extraction pond held with a cellulose membrane; filling the spare volume with Ottawa sand; extracting the diethyl yellow by using ASE (Accelerated Solvent Extraction) by using acetonitrile water solution with volume concentration of 75-85 percent as an extracting agent; evaporating, concentrating and extracting the solution until to dry, adding acetonitrile to dissolve, centrifuging, weighing supernatant, adding sodium chloride solution, oscillating and centrifuging, wherein the supernatant is to-be-tested sample solution; adding gold nanosol and an inorganic salt flocculant into the to-be-tested sample solution, uniformly shaking, and detecting the sample raman spectrum chart by using a raman spectrometer; performing qualitative and quantitative determination. The method has the characteristics of high extraction speed, full extraction, easiness in operation, accurate detection, quickness, no damage and low cost, and foods with problems can be effectively distinguished.

The invention provides a lithium niobate optical sensor and method based on Bloch surface waves. The lithium niobate optical sensor sequentially comprises an optical prism, a periodic photonic crystaland a lithium niobate light absorption dielectric layer from bottom to top. The periodic photonic crystal is formed by alternately arranging a high-refractive-index material and a low-refractive-index material for N periods. The BSW is excited through the multilayer film structure, and an electric field which is strong enough is formed at the light absorption dielectric layer, so that the lithiumniobate can strongly absorb light waves with the frequency of the BSW, and a resonance peak is formed in a reflection spectrum. A relationship between the formant wavelength or azimuth angle and therefractive index of the to-be-detected sample is established, and dynamic detection and qualitative analysis of the refractive index of the to-be-detected sample are realized by detecting the drift ofthe formant wavelength or angle. The sensor is simple to manufacture, can detect an object to be detected repeatedly in real time, and is a photonic crystal refractive index sensor with high sensitivity and high quality factor.

The invention discloses a visual cascade amplification functional nucleic acid sensor for quantitatively detecting salmonellae. A preparation method of the sensor comprises the following steps: extracting strain DNA; developing and screening a primer for an RPA reaction; optimizing reaction time and a reaction temperature of the RPA reaction and determining primer concentration; and optimizing a TdT reaction condition. By the label-free visual cascade amplification functional nucleic acid sensor for sensitively and selectively detecting the salmonellae, which is designed by the method disclosed by the invention, specificity detection on the salmonellae can be completed in short time; and the visual cascade amplification functional nucleic acid sensor has the advantages of visualization, high sensitivity, high universality and the like.

The invention provides a method for detecting purity of perfluoroisobutyronitrile in a perfluoroisobutyronitrile mixed gas, and the method is as follows: detecting the to-be-tested perfluoroisobutyronitrile mixed gas by using a gas chromatography-mass spectrometer to obtain impurity gas components and estimated volume fractions; configuring standard gases according to the estimated volume fractions; detecting the standard gases by a gas chromatograph or the gas chromatography-mass spectrometer, and using an external standard method to obtain standard curves and fitting Equations of impurity gases; detecting the to-be-tested perfluoroisobutyronitrile mixed gas again, and using the fitting equations to obtain volume fractions of all the impurity gases in the to-be-tested perfluoroisobutyronitrile mixed gas; and based on the volume fractions of the impurity gases, obtaining the purity of the perfluoroisobutyronitrile in the mixed gas to be tested. The new method for detecting the purity of the perfluoroisobutyronitrile in the perfluoroisobutyronitrile mixed gas is accurate and reliable, simple in operation and convenient in calculation.

The invention relates to a construction method for a zebra fish osteoporosis model and application thereof. The construction method for the zebra fish osteoporosis model mainly comprises the steps ofzebra fish selection, CdCl2 solution treatment, cartilage dyeing and hard bone dyeing. Compared with a conventional molding method using prednisolone and dexamethasone, the method has the advantages that the teratogenesis rate and the fatality rate of zebra fishes can be remarkably reduced by using a CdCl2 solution for treating the zebra fishes; when the high-concentration CdCl2 solution is used for treating the zebra fishes, the mineralization area of zebra fish skeletons is remarkably reduced, the density of the zebra fish skeletons is remarkably reduced, skeletal development defects are caused, and expression of genes related to skeletal regulation and control is reduced; the method has the advantages of being simple, convenient, quick, high in success rate, good in repeatability, stable, reliable and the like, and can achieve qualitative analysis and quantitative analysis; and the constructed zebra fish model has more outstanding and obvious osteoporosis characteristics, and can bequickly and efficiently used for widely evaluating and screening different types of models for treating osteoporosis drugs.

The invention discloses a qualitative and quantitative determination method for active proteins in a pertussis toxin product and a diphtheria-pertussis-tetanus vaccine. A high-performance liquid chromatography-tandem mass spectrometry method is adopted for establishing the high-flux high-selectivity high-sensitivity quantitative method for active proteins including pertussis toxin, filamentous hemagglutinin, adhesin, mycoprotein and adenylate cyclase toxin in the pertussis toxin product and the diphtheria-pertussis-tetanus vaccine. According to the method, characteristic peptide fragments which can be used for qualitative and quantitative analysis of various active vaccine proteins are screened out from a complex vaccine matrix for the first time, and the peptide fragments are different from other reported protein peptide fragments, cannot be obtained through a protein search library and cannot also be simply obtained through amino acid sequences of the vaccine proteins. Simultaneous quantification of five pertussis toxin subunits, filamentous hemagglutinin, adhesin, mycoprotein and adenylate cyclase toxin in pertussis toxin products and diphtheria-pertussis-tetanus vaccines from different manufacturers and of different batches can be realized.

The invention discloses a detecting method for a hydroxyl ester monomer in a polycarboxylic acid slump retaining agent. The detecting method comprises the following steps: (1) performing qualitative detection: adopting a gas chromatographic method to acquire a gas chromatographic data of a hydroxyl ester monomer reference substance, adopting ethyl acetate to extract a polycarboxylic acid slump retaining agent sample, testing an extract liquor under the same gas chromatographic condition, and qualitatively detecting the category of the hydroxyl ester monomer in the polycarboxylic acid slump retaining agent once the retention time component consistent to the hydroxyl ester monomer appears; (2) performing quantitative detection according to the following steps: a) selecting a group of polycarboxylic acid slump retaining agent sample with known content of the hydroxyl ester monomer, performing alkaline hydrolysis and acid neutralization, and then precisely adding a defined amount of internal standard substance, dimethyl sebacate, and diluting with ethyl alcohol, thereby acquiring a group of known sample to-be-detected liquid; b) adopting a gas chromatographic method to detect the content of the alkaline hydrolysis product in the group of known sample to-be-detected liquid and drawing a standard curve; c) adopting the gas chromatographic method for detecting the content of the alkaline hydrolysis product in the to-be-detected polycarboxylic acid slump retaining agent, and seeking the corresponding content of the hydroxyl ester monomer on the standard curve.

The invention relates to the technical field of lithium ion battery detection, and particularly provides a lithium ion battery short-circuit test method. The method comprises the following steps: obtaining a diaphragm from a short-circuited or micro-short-circuited battery cell and carrying out drying treatment to obtain a dried diaphragm; finding a suspicious point on the dry diaphragm; paving the dry diaphragm with the suspicious points on the surface of the conductor to ensure that the dry diaphragm is in good contact with the conductor and the surface with the suspicious points faces upwards; testing the current value of the dry diaphragm on the surface of the conductor by using detection equipment, measuring the current value at a non-suspicious point of the dry diaphragm, and recording the current value as IR; measuring a current value of the dry diaphragm with a suspicious point, and recording the current value as IW; if IW is greater than IR, judging that the suspicious point is a short-circuit point or a micro short-circuit point; and if IW is equal to IR, judging that the suspicious point is a non-short-circuit point or a non-micro-short-circuit point. According to the method, qualitative and quantitative analysis can be effectively carried out on the short-circuit or micro-short-circuit battery cell, so that the root cause of the short circuit or micro-short circuitcan be found.

The invention provides a quartz crystal microbalance biosensing method based on a mass load reduction method. A biomolecular probe (such as an antibody and a single-stranded DNA) is modified on a quartz crystal, a detection solution (such as pathogenic bacteria, DNA molecules to be detected and the like) is added to the surface of a sensor, a frequency spectrum scanning system is developed by adopting a direct digital synthesis (DDS) technology, and scanning excitation is carried out on a quartz crystal oscillator according to a preset frequency and sine wave amplitude; and a quartz crystal frequency signal acquisition and analysis system is constructed based on a superheterodyne receiving orthogonal phase-sensitive detection technology, and a QCM response signal amplitude-frequency and phase-frequency characteristic curve is drawn. Quartz crystal microbalance biosensing by a mass load reduction method is realized through a QCM response signal spectrum. According to the method, a detection signal is obtained in situ in the detection liquid, and a quartz crystal microbalance is directly adopted for biosensing detection, so that the method has important application value for medicalinstant detection and on-site rapid detection.

The invention relates to an aflatoxin biosynthesis pathway targeted metabolome analysis method, comprising the steps of feeding a sample, performing liquid chromatography-mass spectrometry detection to obtain raw data comprising primary and secondary mass spectrometry information, and performing qualitative analysis referring to a mass spectrometry standard library to obtain qualitative results ofcompounds with standard spectrum; generating a multi-stage mass spectrometric fragmentation tree according to mass spectrum information of the compounds with standard spectrum, generating a multi-stage mass spectrometry tree according to other aflatoxin biosynthesis pathway compounds without standard spectrum in a primary mass spectrometry mass-to-charge ratio sample, and determining the qualityof the compounds without standard spectrum based on the mass spectrometric fragmentation rule according to the discipline of similar structures and similar mass spectrometric behaviors of adjacent compounds in a biosynthesis pathway, thereby completing the qualitative analysis of unknown compounds.

The invention relates to a rapid detection technology of ganoderic acid G in ganoderma lucidum spore oil, in particular to a detection method adopting Raman spectrum characteristic peak signals, and the method is suitable for qualitative detection of ganoderic acid G in ganoderma lucidum spore oil. Raman spectrum characteristic peak information is adopted, 555cm<-1> (+ / -3cm<-1 >) and 1176cm<-1> (+ / -3cm<-1>) are selected as characteristic Raman peaks, qualitative analysis is accurate, the whole detection process is completed within 15min, and rapid qualitative, convenient and low-cost detectionis achieved.

The invention relates to the field of Terahertz devices, and aims at providing a superstructure based adjustable terahertz resonant cavity and method using the resonant cavity for substance analysis.Existing research accomplishments of meta-materials can be used to realize qualitative and quantitative analysis on a substance to be detected. The superstructure based adjustable terahertz resonant cavity is characterized by comprising a fixed plate module, a movable plate module which can move relative to the fixed plate module and a spacing adjusting module which drives the movable plate module; the fixed plate module comprises a static plate support fixed at one end of a linear guide rail and a parallel plate supported by the static plate support and attached with a Terahertz super structure in the surface; the movable plate module comprises a moving plate support which can be located on the linear guide rail in a slidable manner via a displacement bench and a parallel plate supportedby the moving plate support and attached with a Terahertz super structure in the surface; and the spacing adjusting module comprises a screw rod arranged in parallel with the linear guide rail and a bidirectional rotation motor driving the screw rod.

The invention belongs to the technical field of biosensors, and particularly relates to a preparation method of a double-ratio electrochemical aptamer sensor based on amplification linear ranges of alinear aptamer and a hairpin aptamer. The method comprises the steps of forming an AQ-rGO composite material through the non-covalent action of AQ and rGO; and fixing the sulfydryl modified hairpin aptamer and linear aptamer on a sensing interface by using AuNPs; wherein one part of the hairpin aptamer comprises an aptamer sequence and carries an electrochemical molecule methylene blue, and the linear aptamer carries a ferrocene electrochemical signal molecule, so that a novel double-ratio electrochemical aptamer sensor is constructed; compared with the hairpin aptamer, the binding effect of the linear aptamer and a target AFB1 of the linear aptamer is easier to generate, so that the concentration of the target AFB1 causing the hairpin aptamer to carry MB signal molecules to change can beused as a concentration point for the qualitative analysis of the AFB1, the qualitative analysis of the AFB1 in food is realized, the detection sensitivity is high, and the stability is good.

The invention provides a multi-target detector based on a multi-type resonance terahertz metasurface. The detector comprises a substrate and a microrod array structure, and the microrod array structure is disposed on the substrate. A first-class resonance unit structure and a second-class resonance unit structure are arranged on the substrate. According to the first-class resonance unit structure, single microrods are taken as unit structures, and weak coupling occurs between the unit structures, so that electromagnetic resonance response excited by each microrod can cover a relatively wide terahertz frequency spectrum range, wherein the terahertz frequency spectrum spectrum range contains fingerprint spectrums of a plurality of to-be-detected objects, so that qualitative analysis can be performed on a mixture. According to the second-class resonance unit structure, composite microrods are taken as unit structures, strong coupling occurs among the microrods in the unit structures, and weak coupling occurs among the unit structures, so that high-quality factor resonance is generated among the microrods with different lengths through strong coupling, and the change of a surrounding medium environment can be responded with high sensitivity, and the mixture can be accurately and quantitatively detected.

The invention discloses a method for determining catalyst coating and ash distribution on an automobile particulate matter catcher, and belongs to the technical field of physical property determination of automobile exhaust purification devices. The determination method comprises the following implementation steps: (1), marking and sampling three-dimensional coordinates; (2), filling a quadrangular pore passage of a sample by a vacuumizing-free cold embedding method; (3), preparing a sample which can be observed by a scanning electron microscope; (4), analyzing the morphology and chemical element surface distribution of the polished surface of the sample; and (5), carrying out image layer superposition processing. The determination method provided by the invention has the advantages of rapidness, intuition, reliability, simplicity, convenience and the like, is mainly applied to qualitative analysis of axial and radial spatial distribution conditions of the catalyst coating and accumulated ash on the automobile particulate filter, and has very important significance for accelerating research, development and application of automobile particulate filter products.

The invention relates to the technical field of African swine fever virus detection and discloses an African swine fever virus fluorescent PCR (Polymerase Chain Reaction) detection reagent and a detection method thereof. The detection reagent comprises a pair of PCR specific primers, a specific probe, an interior label probe and a PCR mixed liquid. The detection reagent adopts an African swine fever virus VP72 gene which is highly conservative as a target gent. The African swine fever virus fluorescent PCR detection reagent and the detection method thereof have the advantages that the Africanswine fever virus fluorescent PCR detection reagent is simple to use, no multiple special instrument are needed, the detection time is short, the detection efficiency is high, multiple operators are not needed, the labor is saved, and the like.

The invention discloses a simulated well experimental device and an experimental method for natural gas hydrate drilling sampling. The device consists of a surface operation system, a drilling fluid circulating system, a pressure controlling and monitoring system and a temperature controlling and monitoring system, a test well can control a pressure field, a temperature field, and a drilling fluid flow field in the well according to the different hosting conditions of a hydrate to set initial conditions for an experiment. The simulated well experimental device is adopted to control the pressure field, the temperature field, the drilling fluid flow field in the well, and a natural gas hydrate drilling sampling method testing experiment is carried out in a drill hole to study the mechanism of influence of temperature and pressure fields in the drill hole on a natural gas hydrate drilling sampling process. The workload of a field experiment is reduced; the influence of uncontrollability of experimental parameters in the field experiment on the experiment is avoided, and a qualitative and quantitative analysis on each datum can be realized; the influence of the temperature and pressure fields in the drill hole on the natural gas hydrate drilling sampling process can be more favorably analyzed.